Plant Molecular Biology最新文献_第3页

A smart multiplexed microRNA biosensor based on FRET for the prediction of mechanical damage and storage period of strawberry fruits.

IF 3.9 2区生物学

Plant Molecular Biology Pub Date : 2025-02-26 DOI: 10.1007/s11103-025-01564-y

Keyvan Asefpour Vakilian

{"title":"A smart multiplexed microRNA biosensor based on FRET for the prediction of mechanical damage and storage period of strawberry fruits.","authors":"Keyvan Asefpour Vakilian","doi":"10.1007/s11103-025-01564-y","DOIUrl":"https://doi.org/10.1007/s11103-025-01564-y","url":null,"abstract":"Today, measuring the concentration of various microRNAs in fruits has been introduced to model the storage conditions of agricultural products. However, there is a limiting factor in the extensive utilization of such techniques: the existing methods for measuring microRNA sequences, including PCR and microarrays, are time-consuming and expensive and do not allow for simultaneous measurement of several microRNAs. In this study, a biosensor based on the Förster resonance energy transfer (FRET) of fluorescence dyes that can lead to the hybridization of oligonucleotide probes labeled with such dyes by using an excitation wavelength has been used to simultaneously measure microRNAs. Three microRNA compounds, i.e., miRNA-164, miRNA-167, and miRNA-399a, which play significant roles in the postharvest characteristics of strawberry fruits were measured. The simultaneous measurement was performed using three fluorescence dyes which exert various emission wavelengths at 570, 596, and 670 nm. In the following, machine learning methods including artificial neural networks (ANNs) and support vector machines (SVMs), with hyperparameter values optimized with the help of metaheuristic optimization algorithms, were used to predict the amount of mechanical loading on strawberry fruits and their storage period having the microRNA concentrations. The results showed that the SVM with Gaussian kernel, which was optimized by the Harris hawks optimization, is capable of predicting the mechanical stress and storage period of strawberry fruits with a coefficient of determination (R2) of 0.89 and 0.92, respectively. The findings of this study reveal the application of combining FRET-based biosensors and machine learning methods in fruit storage quality assessment.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"115 2","pages":"37"},"PeriodicalIF":3.9,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143516386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Barley young leaf chlorina, a putative pentatricopeptide repeat gene, is essential for chloroplast development in young leaves.

IF 3.9 2区生物学

Plant Molecular Biology Pub Date : 2025-02-25 DOI: 10.1007/s11103-025-01561-1

Biguang Huang, Daiqing Huang, Jianchun Zhang, Jiwei Xiong, Shiyu Wu, Xinrong Zheng, Likun Huang, Jinbin Lin, Yu Li, Zonglie Hong, J Allan Feurtado, Weiren Wu

{"title":"Barley young leaf chlorina, a putative pentatricopeptide repeat gene, is essential for chloroplast development in young leaves.","authors":"Biguang Huang, Daiqing Huang, Jianchun Zhang, Jiwei Xiong, Shiyu Wu, Xinrong Zheng, Likun Huang, Jinbin Lin, Yu Li, Zonglie Hong, J Allan Feurtado, Weiren Wu","doi":"10.1007/s11103-025-01561-1","DOIUrl":"https://doi.org/10.1007/s11103-025-01561-1","url":null,"abstract":"A spontaneous barley mutant, young leaf chlorina (ylc), was identified in this study. Young leaves of ylc exhibited a yellow base and green tip, with reduced chlorophyll content, and altered chlorophyll fluorescence pattern, and underdeveloped grana in chloroplasts. The color of mutant leaves gradually transitioned to pale green and then became green in mature leaves. The ylc phenotype was found to be controlled by a recessive locus mapped to a 2.4 Mb interval on chromosome 7HS using bulked-segregant analysis with deep sequencing and further fine mapped to a 410 kb interval using polymorphic markers. The YLC locus co-segregated with a molecular marker that led to identification of HORVU7Hr1G011570 as the most likely candidate gene. As compared to the barley reference genome, the candidate ylc allele contained nucleotide changes that would lead to functional alterations of its protein product. The candidate YLC gene encodes a DYW-type pentatricopeptide repeat (PPR) protein, implicated in RNA cleavage and RNA editing in chloroplasts. Chlorophyll fluorescence analysis suggests that the PPR protein may regulate chloroplast development through the function of NAD(P)H dehydrogenase (NDH) complex and plays a pivotal role in mediating electron flow in thylakoid membranes during leaf growth in barley.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"115 2","pages":"36"},"PeriodicalIF":3.9,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143503379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tomato miR398 knockout disrupts ROS dynamics during stress conferring heat tolerance but hypersusceptibility to necrotroph infection.

IF 3.9 2区生物学

Plant Molecular Biology Pub Date : 2025-02-24 DOI: 10.1007/s11103-025-01563-z

Shreya Chowdhury, Ananya Mukherjee, Raghuvir Singh, Sushmita Talukdar, Shrabani Basak, Rohit Das, Sayan Mal, Pallob Kundu

{"title":"Tomato miR398 knockout disrupts ROS dynamics during stress conferring heat tolerance but hypersusceptibility to necrotroph infection.","authors":"Shreya Chowdhury, Ananya Mukherjee, Raghuvir Singh, Sushmita Talukdar, Shrabani Basak, Rohit Das, Sayan Mal, Pallob Kundu","doi":"10.1007/s11103-025-01563-z","DOIUrl":"https://doi.org/10.1007/s11103-025-01563-z","url":null,"abstract":"An imbalance between ROS production and scavenging during stress results in oxidative bursts, which causes cellular damage. miR398 is a regulator of ROS scavenging since it targets crucial Cu/Zn superoxide dismutases (CSDs). Established functional studies aligned miR398 with plants' heat and heavy metal stress fitness. However, a knowledge gap in the dynamics of miR398-CSD interaction for redox regulation during pathogenic development impeded their use in crop improvement programmes. We use tomato, Solanum lycopersicum, plants, and necrotrophic and biotrophic pathogens to show that a complex transcriptional and post-transcriptional regulatory circuit maintains SlmiR398 and its target SlCSD genes' level. The interaction is indispensable for ROS regulation in either the pathogenic outcome, thermal stress, or a combination of both stresses, as observed in the cultivation field. The SlmiR398 knockout plants display feeble O2∙- accumulation but enhanced levels of H2O2, several defense-related genes, metabolites, and vital HSFs and HSPs, which were heightened upon stress. Depletion of SlmiR398, although it renders thermotolerance and resilience to biotrophic pathogens likely due to the augmented hypersensitive response, facilitates necrotrophy. Thus, SlmiR398-mediated ROS regulation seemingly works at the interface of abiotic and biotic stress response for a sustainable reaction of tomato plants.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"115 2","pages":"35"},"PeriodicalIF":3.9,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143483907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dwarfs standing tall: breeding towards the 'Yellow revolution' through insights into plant height regulation.

IF 3.9 2区生物学

Plant Molecular Biology Pub Date : 2025-02-19 DOI: 10.1007/s11103-025-01565-x

Shankar K Bhujbal, Archana N Rai, Archana Joshi-Saha

{"title":"Dwarfs standing tall: breeding towards the 'Yellow revolution' through insights into plant height regulation.","authors":"Shankar K Bhujbal, Archana N Rai, Archana Joshi-Saha","doi":"10.1007/s11103-025-01565-x","DOIUrl":"10.1007/s11103-025-01565-x","url":null,"abstract":"High oilseed production is an exigency due to the increasing edible oil consumption of the growing population. Rapeseed and mustard are cultivated worldwide and contribute significantly to the world's total oilseed production. Already a plateau is reached in terms of area and yield in most of the existing cultivars. Most of the commercially cultivated high yielding rapeseed and mustard varieties are tall, mainly due to a wider use of heterosis. However, they are susceptible to lodging and consequent yield losses. Plant yield is strongly dependent upon its architecture; therefore, 'ideotype breeding' is the key approach adopted to develop new varieties with enhanced yield potential, which is less explored in these crops. Dwarf/ semi dwarf plant type varieties has shown its improved yield potential over tall plant type in cereals which further leads to 'Green revolution' in Asian countries. Although, many induced dwarf mutants in rapeseed and mustard were isolated, unlike dwarf green-revolution varieties of cereals, most of them had undesirable plant types with defects including extreme dwarfism and sterility, leading to poor yield potential. Understanding the genetic and molecular mechanisms governing plant height and its correlation with yield and yield contributing characters is crucial. In this review, recent insights into genetic, molecular, and anatomical regulation of plant height have been discussed. The role of hormones, their crosstalk, and hormonal control for cell division and expansion have been delineated with respect to plant architecture. Many dwarfing genes are identified as being part of various phytohormone pathways. Parallelly, molecular links between plant height and flowering time have been explored. The overall synthesis of the review points out some key target pathways and genes that will be useful for plant breeders as well as biotechnologists for targeted genome editing for improving plant architecture without a yield penalty.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"115 2","pages":"34"},"PeriodicalIF":3.9,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11839727/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143458528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

WRKY transcription factor MdWRKY71 regulates flowering time in apple.

IF 3.9 2区生物学

Plant Molecular Biology Pub Date : 2025-02-13 DOI: 10.1007/s11103-024-01544-8

Mengyu Su, Yi Yang, Caicai Lin, Wenjun Liu, Xuesen Chen

{"title":"WRKY transcription factor MdWRKY71 regulates flowering time in apple.","authors":"Mengyu Su, Yi Yang, Caicai Lin, Wenjun Liu, Xuesen Chen","doi":"10.1007/s11103-024-01544-8","DOIUrl":"10.1007/s11103-024-01544-8","url":null,"abstract":"In plants, flowering is crucial to reproductive success. Receiving limited attention in apple research is the function of WRKY transcription factors in regulating flowering time. We characterized a WRKY transcription factor, MdWRKY71, from red-fleshed apple in this study, and ectopically expressed it in Arabidopsis thaliana, which revealed its role in flowering. The sequence of MdWRKY71 exhibited similarity to that of AtWRKY71, and its protein comprised a WRKY domain and a C2H2 zinc finger-like motif, placing it within subgroup IIc of the WRKY family. The similar changing trends demonstrated a significant positive correlation between the expression level of MdWRKY71 and the key flower transition genes in apical buds of apple in flower transition stage. Overexpression of MdWRKY71 promoted the upregulation of certain flower transition genes in apple calli. The ectopic expression of MdWRKY71 in A. thaliana was observed to induce early flowering. Additionally, MdWRKY71 could bind to the promoters of several floral pathway integrators directly and interact with them to enhance their expression levels. These results contribute to our understanding of the molecular mechanism through which MdWRKY71 regulates the flowering process in fruit trees, such as red-fleshed apple.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"115 2","pages":"32"},"PeriodicalIF":3.9,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143409853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and expression pattern analysis of BpGRAS gene family in Bergenia purpurascens and functional characterization of BpGRAS9 in salt tolerance.

IF 3.9 2区生物学

Plant Molecular Biology Pub Date : 2025-02-13 DOI: 10.1007/s11103-025-01562-0

Xin Lyu, Nuomei Xu, Jie Chen, Wenqing Wang, Feiyang Yan, Zongxiang Jiang, Qiankun Zhu

{"title":"Identification and expression pattern analysis of BpGRAS gene family in Bergenia purpurascens and functional characterization of BpGRAS9 in salt tolerance.","authors":"Xin Lyu, Nuomei Xu, Jie Chen, Wenqing Wang, Feiyang Yan, Zongxiang Jiang, Qiankun Zhu","doi":"10.1007/s11103-025-01562-0","DOIUrl":"10.1007/s11103-025-01562-0","url":null,"abstract":"Bergenia purpurascens is an important medicinal, edible, and ornamental plant. It generally grows in extreme environments with complex stresses. The GRAS transcription factors play a crucial role in regulating plant stress tolerance and growth-development. There is no research on GRAS transcription factors in B. purpurascens. In this study, 29 B. purpurascens GRAS (BpGRAS) genes were identified based on B. purpurascens transcriptome data. These BpGRAS genes were classified into seven subfamilies according to phylogenetic analysis, while BpGRAS1 was not classified into any other subfamilies. The motif analysis showed that the protein motifs in the same subfamily were relatively conserved. The expression pattern analysis of BpGRAS genes in different tissues and under salt stress showed that eight BpGRAS genes were differentially expressed under salt stress. The expression profiles showed that BpGRAS9 might play an important role in salt response and the transgenic Arabidopsis thaliana lines with overexpressed BpGRAS9 showed the enhanced salt tolerance. Root length and fresh weight were significantly increased in transgenic lines under salt conditions. The studies enhanced our comprehension of the function of BpGRAS and established a more foundation for exploring the molecular mechanisms underlying plant salt tolerance.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"115 2","pages":"33"},"PeriodicalIF":3.9,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143409677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Folate depletion impact on the cell cycle results in restricted primary root growth in Arabidopsis.

IF 3.9 2区生物学

Plant Molecular Biology Pub Date : 2025-02-13 DOI: 10.1007/s11103-025-01554-0

Jolien De Lepeleire, Ratnesh Chandra Mishra, Jana Verstraete, Jose Antonio Pedroza Garcia, Christophe Stove, Lieven De Veylder, Dominique Van Der Straeten

{"title":"Folate depletion impact on the cell cycle results in restricted primary root growth in Arabidopsis.","authors":"Jolien De Lepeleire, Ratnesh Chandra Mishra, Jana Verstraete, Jose Antonio Pedroza Garcia, Christophe Stove, Lieven De Veylder, Dominique Van Der Straeten","doi":"10.1007/s11103-025-01554-0","DOIUrl":"10.1007/s11103-025-01554-0","url":null,"abstract":"Folates are vital one carbon donors and acceptors for a whole range of key biochemical reactions, including the biosynthesis of DNA building blocks. Plants use one carbon metabolism as a jack of all trades in their growth and development. Depletion of folates impedes root growth in Arabidopsis thaliana, but the mechanistic basis behind this function is still obscure. A global transcriptomic study hinted that folate depletion may cause misregulation of cell cycle progression. However, investigations on a direct connection thereof are scarce. We confirmed the effect of methotrexate (MTX), a folate biosynthesis inhibitor, on the expression of cell cycle genes. Subsequently, we determined the effect of MTX on root morphology and cell cycle progression through phase-specific cell cycle reporter analyses. Our study reveals that folate depletion affects the expression of cell cycle regulatory genes in roots, thereby suppressing cell cycle progression. We confirmed, through DNA labelling by EdU, that MTX treatment leads to arrest in the S phase of meristematic cells, likely due to the lack of DNA precursors. Further, we noted an accumulation of the A-type CYCA3;1 cyclin at the root tip, suggesting a possible link with the observed loss of apical dominance. Overall, our study shows that the restricted cell division and cell cycle progression is one of the reasons behind the loss of primary root growth upon folate depletion.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"115 2","pages":"31"},"PeriodicalIF":3.9,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11825618/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143409702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of the sucrose synthase gene in promoting thorn occurrence and vegetative growth in Lycium ruthenicum.

IF 3.9 2区生物学

Plant Molecular Biology Pub Date : 2025-02-07 DOI: 10.1007/s11103-025-01560-2

Wenhui Liu, Weiman Xu, Yue Gao, Xinyu Qi, Fuqiang Liu, Jiawen Wang, Lujia Li, Yuliang Zhou, Wenxin Chen, Yingyue Jiang, Jianguo Cui, Yucheng Wang, Qin-Mei Wang

{"title":"The role of the sucrose synthase gene in promoting thorn occurrence and vegetative growth in Lycium ruthenicum.","authors":"Wenhui Liu, Weiman Xu, Yue Gao, Xinyu Qi, Fuqiang Liu, Jiawen Wang, Lujia Li, Yuliang Zhou, Wenxin Chen, Yingyue Jiang, Jianguo Cui, Yucheng Wang, Qin-Mei Wang","doi":"10.1007/s11103-025-01560-2","DOIUrl":"10.1007/s11103-025-01560-2","url":null,"abstract":"Lycium ruthenicum is a highly valued ecological and economic shrub, but its abundant thorns disrupt production processes. Previous studies suggested that the sucrose synthase gene (LrSUS) in L. ruthenicum may influence thorn occurrence, presenting potential for breeding thornless varieties suited for cultivation. To explore this, the full-length CDS of LrSUS was cloned, and a novel stable genetic transformation system mediated by Agrobacterium tumefaciens was developed. Through this system, both LrSUS overexpression and suppression lines were generated. While suppression lines exhibited slow growth and failed to survive post-transplant, overexpression lines demonstrated accelerated growth, with significant increases in adventitious root number and length. Upon transplanting, the overexpression lines also showed enhanced thorn occurrence, alongside notable increases in thorn length, leaf size, stem diameter, photosynthetic rate, and sugar content. Subcellular localization analysis using a transient expression method based on the injection of L. ruthenicum indicated that the LrSUS gene product is localized in the chloroplasts. Key genes involved in LrSUS/ sucrose affecting thorn occurrence event were identified through high throughput transcriptome analysis and a hypothetical mechanistic model was established. This study provides valuable insights into the function of LrSUS and establishes a foundation for manipulating thorn phenotypes in L. ruthenicum and related species.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"115 1","pages":"30"},"PeriodicalIF":3.9,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143365351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Virtual staining from bright-field microscopy for label-free quantitative analysis of plant cell structures.

IF 3.9 2区生物学

Plant Molecular Biology Pub Date : 2025-01-31 DOI: 10.1007/s11103-025-01558-w

Manami Ichita, Haruna Yamamichi, Takumi Higaki

{"title":"Virtual staining from bright-field microscopy for label-free quantitative analysis of plant cell structures.","authors":"Manami Ichita, Haruna Yamamichi, Takumi Higaki","doi":"10.1007/s11103-025-01558-w","DOIUrl":"10.1007/s11103-025-01558-w","url":null,"abstract":"The applicability of a deep learning model for the virtual staining of plant cell structures using bright-field microscopy was investigated. The training dataset consisted of microscopy images of tobacco BY-2 cells with the plasma membrane stained with the fluorescent dye PlasMem Bright Green and the cell nucleus labeled with Histone-red fluorescent protein. The trained models successfully detected the expansion of cell nuclei upon aphidicolin treatment and a decrease in the cell aspect ratio upon propyzamide treatment, demonstrating its utility in cell morphometry. The model also accurately documented the shape of Arabidopsis pavement cells in both wild type and the bpp125 triple mutant, which has an altered pavement cell phenotype. Metrics such as cell area, circularity, and solidity obtained from virtual staining analyses were highly correlated with those obtained by manual measurements of cell features from microscopy images. Furthermore, the versatility of virtual staining was highlighted by its application to track chloroplast movement in Egeria densa. The method was also effective for classifying live and dead BY-2 cells using texture-based machine learning, suggesting that virtual staining can be applied beyond typical segmentation tasks. Although this method still has some limitations, its non-invasive nature and efficiency make it highly suitable for label-free, dynamic, and high-throughput analyses in quantitative plant cell biology.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"115 1","pages":"29"},"PeriodicalIF":3.9,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11782351/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143067221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Directed evolution of diacylglycerol acyltransferases 2 promotes lipids and triglyceride accumulation.

IF 3.9 2区生物学

Plant Molecular Biology Pub Date : 2025-01-23 DOI: 10.1007/s11103-025-01552-2

Tinghui Feng, Qiang Zhang, Tong Gao, Jiacong Gao, Jiahao Fang, Xiaodan Zhang, Juane Dong, Zongsuo Liang

{"title":"Directed evolution of diacylglycerol acyltransferases 2 promotes lipids and triglyceride accumulation.","authors":"Tinghui Feng, Qiang Zhang, Tong Gao, Jiacong Gao, Jiahao Fang, Xiaodan Zhang, Juane Dong, Zongsuo Liang","doi":"10.1007/s11103-025-01552-2","DOIUrl":"10.1007/s11103-025-01552-2","url":null,"abstract":"Triacylglycerol (TAG) is a major component of plant-neutral lipids. Diacylglycerol acyltransferase 2 (DGAT2) plays an important role in plant oil accumulation by catalyzing the final step of the Kennedy pathway. In this study, ten DGAT2 sequences were originating from different oil crops into the TAG-deficient yeast strain H1246, to compare their enzyme activity of oil synthesis and filter out potential amino acid residue sites for directed evolution. Based on the synthesis efficiency of total lipids, TAGs, and the topology models of these DGAT2s, five possible amino acid sites were identified that may affect the synthesis of total lipids and TAGs. In the H1246 yeast expression system, HaDGAT2 significantly increased the total oil and TAG content; however, ClDGAT2 was weak in synthesizing both oil and TAG. Thus, building on HaDGAT2 and ClDGAT2, these amino acid substitutions were created by point-to-point mutating and substantially affected the oil or TAG synthesis ability of DGAT2s. Among the five amino acid substitutions, mutations at residue (3) successfully make HaDGAT2 less capable of synthesizing lipids and TAG, and ClDGAT2 more capable of synthesizing total lipids and TAG. Except mutations at residue (2), all residue mutations contributed to a weaker ability of fatty acid synthesis. In addition, ten mutant DGAT2s and two parental DGAT2s were overexpressed in tobacco leaves to reveal their lipid synthesis function. This approach helped us to authenticate the significance of these loci. In varying degrees, those mutations enhanced the ability of ClDGAT2 to synthesize lipids, attenuated the ability of HaDGAT2 to synthesize lipids, and altered preference for fatty acids in tobacco.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"115 1","pages":"28"},"PeriodicalIF":3.9,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143029258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0