Plant Molecular Biology最新文献_第3页

Exogenous ABA regulates stress tolerance of Leymus chinensis via modulation of PYL‑mediated signaling and antioxidant enzymes. 外源ABA通过调节PYL介导的信号和抗氧化酶调控羊草的逆境耐受性。

IF 3.7 2区生物学

Plant Molecular Biology Pub Date : 2026-08-25 DOI: 10.1007/s11103-026-01753-3

Tao Sun, Yue Liu, Jingying Dong, Ruiqi Zhao, Haiyan Li, Bo Lei, Kun Cao, Yingjie Yu

{"title":"Exogenous ABA regulates stress tolerance of Leymus chinensis via modulation of PYL‑mediated signaling and antioxidant enzymes.","authors":"Tao Sun, Yue Liu, Jingying Dong, Ruiqi Zhao, Haiyan Li, Bo Lei, Kun Cao, Yingjie Yu","doi":"10.1007/s11103-026-01753-3","DOIUrl":"10.1007/s11103-026-01753-3","url":null,"abstract":"As an important species for ecological restoration and high-quality forage in northern China, Leymus chinensis has drawn considerable research interest regarding its stress tolerance regulatory mechanisms. Exogenous abscisic acid (ABA), a key stress-signaling hormone, can effectively pre-activate the antioxidant defense system in L. chinensis. To elucidate the ABA response mechanism, this study combined physiological assessments-including antioxidant enzyme activities-with transcriptome sequencing of ABA-treated plants. The results demonstrated that ABA treatment enhanced the activities of peroxidase, superoxide dismutase, and catalase in leaves while reducing malondialdehyde content. Transcriptomic analysis further revealed that ABA induced extensive gene expression reprogramming, with significant enrichment in pathways such as plant hormone signal transduction. Subsequently, 21 PYL gene family members (LcPYLs) were identified for the first time in the L. chinensis genome. Systematic characterization indicated that these genes possess conserved structures, contain promoter regions enriched in stress-responsive elements such as ABRE, and are phylogenetically closer to PYLs in rice. Expression profiling confirmed that multiple LcPYLs were significantly upregulated under both exogenous ABA and low-temperature stress. Collectively, this study reveals that exogenous ABA may improve stress tolerance in L. chinensis, which is potentially associated with the activation of the antioxidant system and PYL-mediated signaling. These findings provide valuable genetic resources and a theoretical reference for future molecular breeding of stress-resilient L. chinensis varieties.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"116 5","pages":""},"PeriodicalIF":3.7,"publicationDate":"2026-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148819188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The AtRNC1-AtWTF1 protein complex is essential for chloroplast group Ⅱ intron splicing, ribosome biogenesis, and embryo development in Arabidopsis. AtRNC1-AtWTF1蛋白复合物对拟南芥叶绿体群Ⅱ内含子剪接、核糖体生物发生和胚胎发育至关重要。

IF 3.7 2区生物学

Plant Molecular Biology Pub Date : 2026-08-24 DOI: 10.1007/s11103-026-01756-0

Yichen Zhang, Xinyun Xu, Yajuan Li, Siwen Wei, Jiayi Shen, Qing-Bo Yu, Yong-Lan Cui

{"title":"The AtRNC1-AtWTF1 protein complex is essential for chloroplast group Ⅱ intron splicing, ribosome biogenesis, and embryo development in Arabidopsis.","authors":"Yichen Zhang, Xinyun Xu, Yajuan Li, Siwen Wei, Jiayi Shen, Qing-Bo Yu, Yong-Lan Cui","doi":"10.1007/s11103-026-01756-0","DOIUrl":"10.1007/s11103-026-01756-0","url":null,"abstract":"The splicing of group Ⅱ introns in chloroplasts is essential for photosynthesis and plant development and relies on a series of nucleus-encoded RNA-binding proteins. Here, we demonstrate that two Arabidopsis proteins, ribonuclease Ⅲ-domain protein AtRNC1 and the Plant organelle RNA recognition (PORR) protein AtWTF1, are essential for chloroplast function. Null mutants of AtRNC1 or AtWTF1 are embryo lethal. We rescued the null mutant by expressing wild-type AtRNC1 under the control of the seed-specific ABSCISIC ACID-INSENSITIVE3 (ABI3) promoter. We also created atrnc1 knockdown plants using artificial-microRNA (amiRNA). Both the rescued and knockdown atrnc1 plants exhibit chlorotic phenotype. Similarly, AtWTF1 hypomorphic mutants display variegated phenotypes. Both proteins are chloroplast-localized, with AtRNC1 residing in the stroma and AtWTF1 in both the stroma and thylakoids. Molecular phenotyping established that AtRNC1 and AtWTF1 are required for the efficient splicing of a specific, overlapping set of chloroplast group Ⅱ introns, including those in petB, petD, rpl2, rps12, and tRNAs. RNA immunoprecipitation confirmed the association of both proteins with their target introns in vivo, indicating direct roles in splicing. The knockdown of AtRNC1 or AtWTF1 in Arabidopsis impairs the chloroplast ribosome accumulation and accordingly reduced the efficiency of mRNA translation. Notably, protein interaction assays, including yeast two-hybrid, luciferase complementation image, and IP-MS indicate that AtRNC1 and AtWTF1 associate with each other, implying their cooperative function in a splicing complex. These findings establish AtRNC1 and AtWTF1 as key components of the chloroplast RNA splicing machinery, essential for the maturation of plastid transcripts and overall chloroplast homeostasis in Arabidopsis.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"116 5","pages":""},"PeriodicalIF":3.7,"publicationDate":"2026-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148813774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The aluminum-activated malate transporter 9 (VaALMT9) promotes malate accumulation in rabbiteye blueberry fruit and is transcriptionally inhibited by VaMYB1R1 and VaWRKY21. 铝激活的苹果酸转运蛋白9 （VaALMT9）促进苹果酸在兔眼蓝莓果实中的积累，并受到VaMYB1R1和VaWRKY21的转录抑制。

IF 3.7 2区生物学

Plant Molecular Biology Pub Date : 2026-08-11 DOI: 10.1007/s11103-026-01755-1

Miao Zhou, Yi Min, Yuxin Leng, Yongfang Dai, Lei Peng, Lin Wang, Xiaoya Zheng, Xiaopeng Wen

{"title":"The aluminum-activated malate transporter 9 (VaALMT9) promotes malate accumulation in rabbiteye blueberry fruit and is transcriptionally inhibited by VaMYB1R1 and VaWRKY21.","authors":"Miao Zhou, Yi Min, Yuxin Leng, Yongfang Dai, Lei Peng, Lin Wang, Xiaoya Zheng, Xiaopeng Wen","doi":"10.1007/s11103-026-01755-1","DOIUrl":"10.1007/s11103-026-01755-1","url":null,"abstract":"Blueberry (Vaccinium spp.) has gained worldwide popularity as a fruit crop of exceptional flavor and high nutritional value. Fruit acidity, a critical determinant of consumer preference in rabbiteye blueberry, is primarily governed by vacuolar malic acid accumulation; however, the underlying regulatory mechanism remains unclear. In the current study, VaALMT9, encoding an aluminum-activated malate transporter, was identified from rabbiteye blueberry transcriptome data. The full-length coding sequence encodes a 574 amino acid protein harboring the characteristic WEP fingerprint motif of the ALMT family. Subcellular localization analysis suggested that VaALMT9 localizes to the endomembrane system, most likely the tonoplast (vacuolar membrane). Expression analysis revealed that VaALMT9 transcript levels paralleled malate accumulation during fruit development, with both peaking at the purple stage and declining at the late ripening stage. Virus-induced gene silencing of VaALMT9 in blueberry fruits significantly reduced its transcript levels (by 96.74%) and malate contents of the two independent silenced lines were remarkably decreased by 0.62 mg/g and 0.50 mg/g, which were reduced by 19.37% and 15.78% in comparison with those of the control. Conversely, VaALMT9-overexpressing tomato markedly increased malate accumulation by 13.79-44.37%. Moreover, yeast one-hybrid and dual-luciferase reporter assays revealed that VaMYB1R1 and VaWRKY21 bind to the VaALMT9 promoter and repress its transcriptional activity. In conclusion, VaALMT9 was identified as a key gene promoting malate accumulation, and is transcriptionally inhibited by VaMYB1R1 and VaWRKY21. The findings provide new insights into the molecular regulation underlying fruit acid accumulation and offer valuable gene targets for genetic improvement of fruit quality in this fruit crop.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"116 5","pages":""},"PeriodicalIF":3.7,"publicationDate":"2026-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148707146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated transcriptome and metabolome analysis of the differential regulation of physiological metabolism in passion fruit by different MeJA concentrations. 不同MeJA浓度对百香果生理代谢差异调控的整合转录组和代谢组分析。

IF 3.7 2区生物学

Plant Molecular Biology Pub Date : 2026-07-30 DOI: 10.1007/s11103-026-01748-0

Manjing Lian, Kaizhen Luo, Qing Luo, Guoying Yan, Fengmei He, Chunya Jiang, Xuelian Yang

{"title":"Integrated transcriptome and metabolome analysis of the differential regulation of physiological metabolism in passion fruit by different MeJA concentrations.","authors":"Manjing Lian, Kaizhen Luo, Qing Luo, Guoying Yan, Fengmei He, Chunya Jiang, Xuelian Yang","doi":"10.1007/s11103-026-01748-0","DOIUrl":"10.1007/s11103-026-01748-0","url":null,"abstract":"This study utilized 'Qinmi No.9' passion fruit to explore pre-harvest methyl jasmonate (MeJA) treatment effects on fruit quality and regulatory mechanisms via physiological assays, transcriptomics, and metabolomics. MeJA delayed peel yellowing, elevated total soluble solids, total flavonoid, soluble protein contents, and polygalacturonase, polyphenol oxidase, and lipoxygenase activities, but decreased titratable acidity, pectin content, and electrical conductivity, with MeJA_10 (1.0 mM MeJA) presenting optimal comprehensive performance. Transcriptomic analysis revealed that MeJA_5 (0.5 mM MeJA) mainly enriched plant hormone signal transduction and glycolysis/gluconeogenesis pathways, while MeJA_10 activated tryptophan metabolism and phenylpropanoid biosynthesis significantly; five hub genes (At2g46620, OGT, ENDOV, SAMHD1, and γ-TMT) were identified by WGCNA. Metabolomic data showed MeJA_10 induced more differentially accumulated metabolites (226) than MeJA_5 (146): MeJA_5 vs CK mainly altered organoheterocyclic compounds and phenylpropanoids/polyketides, while MeJA_10 vs CK predominantly regulated lipids/lipid-like molecules and organic acids/derivatives. Integrated analysis demonstrated that MeJA_5 enriched glycolysis/gluconeogenesis and α-linolenic acid metabolism, and MeJA_10 highly enriched starch/sucrose metabolism, tryptophan metabolism, and phenylpropanoid biosynthesis. MeJA_10 triggered more differentially expressed genes and metabolites than MeJA_5, confirming its stronger activation of metabolic and genetic expression for better fruit quality promotion.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"116 4","pages":""},"PeriodicalIF":3.7,"publicationDate":"2026-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148631336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cross-stress memory in plants: mobile RNAs, extracellular vesicles, and local-to-systemic signal integration. 植物的交叉胁迫记忆：移动rna、细胞外囊泡和局部到系统的信号整合。

IF 3.7 2区生物学

Plant Molecular Biology Pub Date : 2026-07-30 DOI: 10.1007/s11103-026-01746-2

Wajid Zaman, Adnan Amin

{"title":"Cross-stress memory in plants: mobile RNAs, extracellular vesicles, and local-to-systemic signal integration.","authors":"Wajid Zaman, Adnan Amin","doi":"10.1007/s11103-026-01746-2","DOIUrl":"10.1007/s11103-026-01746-2","url":null,"abstract":"Plants rarely experience environmental stresses as isolated events. Instead, they are exposed to recurring, overlapping, or sequential challenges that require not only immediate local responses but also coordinated systemic adaptation and memory. Although plant stress memory has traditionally been discussed in terms of transcriptional, epigenetic, and metabolic reprogramming, accumulating evidence suggests that long-distance communication is equally important in determining how prior stress exposure shapes future responses. In this context, mobile RNAs and extracellular vesicles (EVs) are emerging as key mediators of local-to-systemic information transfer. Mobile RNAs, including small RNAs, mRNAs, long non-coding RNAs, and related RNA species, can move across cells, tissues, organs, and even species boundaries to regulate stress adaptation, whereas plant EVs carry complex molecular cargoes that participate in intercellular signalling, defense, and stress-responsive communication. Recent studies further indicate that EV secretion is stress-inducible and that plant EVs can amplify immune signalling during systemic acquired resistance (SAR), highlighting their potential contribution to plant-wide memory-like states. This review proposes that cross-stress memory should be reconsidered as a systemic signaling phenomenon, in which local stress encounters generate transmissible molecular information that reshapes distal tissue responsiveness and influences later reactions to the same or different stresses. We synthesize current knowledge on plant stress memory, RNA mobility, EV biogenesis and cargo, and systemic signalling networks, with emphasis on how these components may converge to establish, propagate, and recall stress information across the plant body. By linking mobile RNAs and EV-mediated communication with recurrent and cross-stress acclimation, this review provides a new conceptual framework for plant stress tolerance and identifies experimental priorities and translational opportunities for crop resilience under dynamic environments.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"116 4","pages":""},"PeriodicalIF":3.7,"publicationDate":"2026-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148631384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

FLC genes control flowering time to varying degrees in a Brassica napus spring cultivar. FLC基因在不同程度上控制着甘蓝型油菜春季品种的开花时间。

IF 3.7 2区生物学

Plant Molecular Biology Pub Date : 2026-07-29 DOI: 10.1007/s11103-026-01741-7

Sarah Duveneck, Kea Ille, Siegbert Melzer

{"title":"FLC genes control flowering time to varying degrees in a Brassica napus spring cultivar.","authors":"Sarah Duveneck, Kea Ille, Siegbert Melzer","doi":"10.1007/s11103-026-01741-7","DOIUrl":"10.1007/s11103-026-01741-7","url":null,"abstract":"In the crop Brassica napus (oilseed rape), distinct growth types have been established that differ mainly in their vernalization requirement for flowering. The need for vernalization in Arabidopsis is controlled by the expression of the floral repressor FLOWERING LOCUS C (FLC), which also regulates cold-responsive flowering in B. napus. Notably, FLC homologs are also retained in spring oilseed rape despite its lack of vernalization requirement. To elucidate the functions of the nine BnFLC homologs in a spring type, we generated CRISPR/Cas9 knockout mutants of all homologs in the cultivar Westar. We show that the loss of BnFLC genes significantly accelerates flowering, demonstrating that BnFLC genes regulate flowering in spring types independently of vernalization. Transcriptomic analyses in leaves revealed distinct expression patterns among the BnFLC genes, with some remaining active during floral transition. Finally, no epigenetic regulation of the BnFLC homologs associated with flowering time was detected, while BnFLC.A03b carried persistent repressive marks and was constitutively silenced. Unexpectedly, additional flowering time regulators, including genes typically active in the shoot apical meristem, were expressed in leaves, with some showing altered expression and chromatin states in the mutant. These findings reveal that BnFLC genes are developmentally regulated and directly control flowering in spring oilseed rape, while also modulating the expression of other floral regulators in B. napus.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"116 4","pages":""},"PeriodicalIF":3.7,"publicationDate":"2026-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13421269/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148620927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ZmSK1 encodes a sucrose synthase essential for starch synthesis and endosperm development in maize. ZmSK1编码玉米淀粉合成和胚乳发育所必需的蔗糖合成酶。

IF 3.7 2区生物学

Plant Molecular Biology Pub Date : 2026-07-25 DOI: 10.1007/s11103-026-01750-6

Shuai Wang, Tianyi Wang, Ruyang Zhang, Zhiyong Li, Qian Liu, Xiaqing Wang, Chunhui Li, Jinfeng Xing, Ronghuan Wang, Wei Song, Jiuran Zhao

{"title":"ZmSK1 encodes a sucrose synthase essential for starch synthesis and endosperm development in maize.","authors":"Shuai Wang, Tianyi Wang, Ruyang Zhang, Zhiyong Li, Qian Liu, Xiaqing Wang, Chunhui Li, Jinfeng Xing, Ronghuan Wang, Wei Song, Jiuran Zhao","doi":"10.1007/s11103-026-01750-6","DOIUrl":"10.1007/s11103-026-01750-6","url":null,"abstract":"Maize (Zea mays) endosperm is the primary reservoir of nutrients that serve as energy and carbon sources for seed germination and early seedling growth. The characterization of endosperm mutants has provided insights into the mechanism underlying endosperm development and resources for maize breeding. In this study, we identified shriveled kernel1 (zmsk1) as a new maize mutant with shriveled kernels containing an opaque and entirely floury endosperm. ZmSK1 was identified as the gene at the Zm00001d045042 locus using a map-based cloning strategy; a specific nucleotide change resulting in aberrant splice sites is responsible for the phenotype of zmsk1 kernels. Allelism tests verified that ZmSK1 is a novel allele of a previously reported gene (Sh1). ZmSK1 was predicted to encode a sucrose synthase localized in the plasma membrane and cytoplasm. An expression pattern analysis indicated that ZmSK1 is ubiquitously expressed in all examined tissues, but especially in kernels and actively growing tissues. Transcriptome analyses indicated that ABC transporter, ribosome and cell expansion-related pathways were significantly enriched, in the zmsk1 mutant, suggesting that a loss-of-function mutation in ZmSK1 may affect protein synthesis, transmembrane transport and early endosperm cell development and morphogenesis, while also inhibiting normal carbohydrate metabolism. Thus, our study provides insights into the molecular basis of maize kernel endosperm development.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"116 4","pages":""},"PeriodicalIF":3.7,"publicationDate":"2026-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148593106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functional characterization of the MdMYB73L gene in drought and salt stress tolerance in apple. MdMYB73L基因在苹果抗旱、耐盐胁迫中的功能研究。

IF 3.7 2区生物学

Plant Molecular Biology Pub Date : 2026-07-22 DOI: 10.1007/s11103-026-01732-8

Xiao-Wen Li, Han-Yu Dong, Ya-Qi Liu, Jing-Yi Su, Xue Wang, Chang Li, Qi-Fei Wu, Wen-Xian Zhang, Xin-Rong Shi, Hong-Jian Cao, Qiangbo Liu, Xiao-Fei Wang, Ting-Ting Zhang

{"title":"Functional characterization of the MdMYB73L gene in drought and salt stress tolerance in apple.","authors":"Xiao-Wen Li, Han-Yu Dong, Ya-Qi Liu, Jing-Yi Su, Xue Wang, Chang Li, Qi-Fei Wu, Wen-Xian Zhang, Xin-Rong Shi, Hong-Jian Cao, Qiangbo Liu, Xiao-Fei Wang, Ting-Ting Zhang","doi":"10.1007/s11103-026-01732-8","DOIUrl":"10.1007/s11103-026-01732-8","url":null,"abstract":"Drought and salt stresses impose serious effects on plant proliferation and development, with multiple categories of transcription factors (TFs) playing important regulatory effects in modulation stress reaction. In this study, the apple R2R3 MYB TF, MdMYB73L, was cloned and characterized its role in conferring abiotic stress tolerance. Firstly, expression assays demonstrated that the transcript levels of MdMYB TFs changed substantially under salt and drought stresses. Subsequently, MdMYB73L-overexpressing transgenic Arabidopsis, apple calli and tomato plants all showed increased sensitivity to salt and drought treatments, manifesting as short roots and severe growth inhibition compared to controls. These results demonstrate that MdMYB73L functions as a key negative regulator in the response to drought and salt stresses. Overall, these research not only demonstrate the specific function of a MYB TF in stress responses but also provide a crucial framework for elucidating the broader effects of the apple MYB family in abiotic stress tolerance.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"116 4","pages":""},"PeriodicalIF":3.7,"publicationDate":"2026-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148550141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phenolamide-driven α-glucosidase inhibitory activity in immature fruits of the Korean landrace-derived chili pepper Sumihyang. 苯酚驱动的α-葡萄糖苷酶抑制活性在韩国地方衍生辣椒sumi香的未成熟果实中。

IF 3.7 2区生物学

Plant Molecular Biology Pub Date : 2026-07-22 DOI: 10.1007/s11103-026-01733-7

Seong-Im Park, Sang Seok Lee, Dae Hong Lee, Hyo Jin Lee, Kil Su Jang

{"title":"Phenolamide-driven α-glucosidase inhibitory activity in immature fruits of the Korean landrace-derived chili pepper Sumihyang.","authors":"Seong-Im Park, Sang Seok Lee, Dae Hong Lee, Hyo Jin Lee, Kil Su Jang","doi":"10.1007/s11103-026-01733-7","DOIUrl":"10.1007/s11103-026-01733-7","url":null,"abstract":"Understanding the biochemical basis of α-glucosidase inhibitory (AGI) activity in chili peppers (Capsicum annuum L.) is essential for their development as functional food ingredients. This study evaluated AGI activity and phytochemical profiles across four ripening stages in three C. annuum cultivars: Sumihyang (a cultivar derived from the Korean landrace Subicho), Dangjo (a functional antidiabetic cultivar), and Olbokhap (a disease-resistant commercial cultivar). Enzyme assays revealed that AGI activity peaked at the immature stage in all cultivars, with Sumihyang consistently exhibiting the highest activity across two harvest periods. Untargeted metabolomic profiling at the immature and turning stages identified two phenolamides, caffeoylputrescine and feruloylputrescine, that were selectively enriched in immature Sumihyang and strongly correlated with AGI activity (r > 0.92). While the AGI effect of caffeoylputrescine has been previously reported, feruloylputrescine was evaluated here for the first time under this assay system. In vitro validation confirmed their inhibitory activities, with half maximal inhibitory concentration (IC50) values of 193 µM for caffeoylputrescine and 291 µM for feruloylputrescine. These findings suggest that phenolamide composition, rather than total flavonoid content, is a key determinant of AGI activity in chili peppers. The selective accumulation of these phenolamides in immature Sumihyang highlights its potential as a genetic resource for developing antidiabetic functional foods.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"116 4","pages":""},"PeriodicalIF":3.7,"publicationDate":"2026-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148550092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Non-coding RNAs as central regulators of phytohormone signaling in plant abiotic stress responses. 非编码rna在植物非生物胁迫反应中作为植物激素信号的中心调控因子。

IF 3.7 2区生物学

Plant Molecular Biology Pub Date : 2026-07-22 DOI: 10.1007/s11103-026-01740-8

Sajid Ali Shah, Alisa Chowhan, Saima Aslam

{"title":"Non-coding RNAs as central regulators of phytohormone signaling in plant abiotic stress responses.","authors":"Sajid Ali Shah, Alisa Chowhan, Saima Aslam","doi":"10.1007/s11103-026-01740-8","DOIUrl":"10.1007/s11103-026-01740-8","url":null,"abstract":"Plants are continuously exposed to a wide range of abiotic stresses, including drought, salinity, temperature extremes, nutrient deficiency, and heavy metal toxicity, which severely constrain growth and productivity. To cope with these challenges, plants have evolved sophisticated regulatory networks involving phytohormones and non-coding RNAs (ncRNAs). This review provides a comprehensive overview of the biogenesis and functional roles of major ncRNA classes' microRNAs (miRNAs), long non-coding RNAs (lncRNAs), and circular RNAs (circRNAs) and their dynamic interplay with phytohormonal signaling pathways under stress conditions. We highlight how ncRNAs modulate key hormonal pathways, including abscisic acid, auxin, jasmonic acid, ethylene, and gibberellins, to fine-tune stress-responsive gene expression and maintain cellular homeostasis. Special emphasis is placed on nutrient stress and heavy metal toxicity, where ncRNA-mediated regulation influences ion transport, autophagy, reactive oxygen species detoxification, and metabolic reprogramming. Emerging evidence on circRNAs as miRNA sponges further reveals an additional regulatory layer linking developmental processes with stress adaptation. The review also integrates recent advances in high-throughput sequencing, multi-omics approaches, and bioinformatics tools that have enabled large-scale identification and functional annotation of stress-responsive ncRNAs. Furthermore, the application of artificial intelligence and machine learning models in predicting ncRNA target interactions and regulatory networks is discussed as a transformative approach in plant stress biology. Collectively, this synthesis provides a systems-level understanding of ncRNA-phytohormone cross-talk and underscores its potential in developing climate-resilient crops through advanced molecular and computational strategies.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"116 4","pages":""},"PeriodicalIF":3.7,"publicationDate":"2026-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148550169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0