Plant Molecular Biology最新文献_第4页

Genomics assisted breeding for mildew resistance in cucumber: from gene discovery to future innovations. 基因组学辅助黄瓜抗霉育种：从基因发现到未来创新。

IF 3.7 2区生物学

Plant Molecular Biology Pub Date : 2026-07-22 DOI: 10.1007/s11103-026-01738-2

Rajinder Kumar Dhall, Neha Rana, Gurpreet Kaur, Sahil Singh Mandyal

{"title":"Genomics assisted breeding for mildew resistance in cucumber: from gene discovery to future innovations.","authors":"Rajinder Kumar Dhall, Neha Rana, Gurpreet Kaur, Sahil Singh Mandyal","doi":"10.1007/s11103-026-01738-2","DOIUrl":"10.1007/s11103-026-01738-2","url":null,"abstract":"Cucumber is an economically important vegetable crop cultivated worldwide, but its productivity is severely affected by destructive foliar diseases particularly powdery mildew and downy mildew. These pathogens cause significant yield and quality losses and the continuous emergence of new races makes disease management increasingly challenging. Conventional approaches including cultural, biological and chemical control often provide limited and short-term effectiveness. Therefore, the development of host plant resistance remains the most sustainable and environmentally sound strategy for long-term disease control. Recent advances in cucumber genomics and molecular breeding have enabled the identification of resistance-associated loci through SNP genotyping, QTL mapping, genome-wide association studies and marker-assisted selection. Furthermore, multi-omics approaches such as transcriptomics, proteomics and metabolomics combined with innovative technologies like CRISPR/Cas-mediated genome editing, genomic selection and speed breeding are transforming resistance breeding. Therefore, by integrating advanced molecular tools with omics-driven insights, this review aims to accelerate genetic gains and facilitate the development of durable, broad-spectrum mildew-resistant cucumber cultivars for sustainable and resilient production systems.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"116 4","pages":""},"PeriodicalIF":3.7,"publicationDate":"2026-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148550180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

QTL mapping for root traits during post-tillering stage under moisture-deficit stress in wheat. 水分亏缺胁迫下小麦分蘖后根系性状的QTL定位

IF 3.7 2区生物学

Plant Molecular Biology Pub Date : 2026-07-21 DOI: 10.1007/s11103-026-01739-1

Sreehari V Santhosh, Sukumar Taria, Sudhir Kumar, Hari Krishna, Karthik Kumar Manjunath, Seetha Laxmi, Arumugam Tamilselvan, Raman Rajasekar, Ezhumalai Sivapragasam, Allimuthu Elangovan, Biswabiplab Singh, Dhandapani Raju, Renu Pandey, Pradeep Kumar Singh, Viswanathan Chinnusamy

{"title":"QTL mapping for root traits during post-tillering stage under moisture-deficit stress in wheat.","authors":"Sreehari V Santhosh, Sukumar Taria, Sudhir Kumar, Hari Krishna, Karthik Kumar Manjunath, Seetha Laxmi, Arumugam Tamilselvan, Raman Rajasekar, Ezhumalai Sivapragasam, Allimuthu Elangovan, Biswabiplab Singh, Dhandapani Raju, Renu Pandey, Pradeep Kumar Singh, Viswanathan Chinnusamy","doi":"10.1007/s11103-026-01739-1","DOIUrl":"10.1007/s11103-026-01739-1","url":null,"abstract":"Root traits during post-tillering stages in wheat are critical for adapting to moisture-deficit stress. This study mapped 24 traditional quantitative trait loci (QTLs) of root and yield traits in wheat across 14 chromosomes using 198 recombinant inbred lines (RILs), developed by crossing two contrasting parents, HD 3086 and HI 1500. Genotyping was done with the 35 K Axiom Wheat Breeder's Array, followed by QTL mapping through Inclusive Composite Interval Mapping (ICIM) software. From the 5 conditional QTLs (Y75|Y45) mapped, QARFC.iari-2 A was mapped as the major stable QTL for root fresh weight, explaining 11.23% of the phenotypic variance, indicating its direct genetic effects during 75 days after sowing. Additionally, the chromosomal region of 6 A (flanked by the markers AX-94646424 and AX-94974293) was linked to six traits indicating its pleiotropy. Candidate gene TraesCS5A03G0005300, an ethylene receptor, was identified in QRSL.iari-5 A, affecting root-shoot length ratio. Multi-trait indices MGIDI (Multi-Trait Genotype-Ideotype Distance Index), FAI-BLUP (Factor Analysis and Ideotype-design with Best Linear Unbiased Prediction), and SHI (Smith-Hazel index) identified three common RILs (RIL-53, RIL-56 and RIL-123) suitable for breeding programmes. These identified QTLs can be utilised for marker-assisted selection (MAS) in future breeding programmes after successful validation.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"116 4","pages":""},"PeriodicalIF":3.7,"publicationDate":"2026-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148536655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A comprehensive exploration of molecular aspects of rice-Ustilaginoidea virens interactions. 水稻与野田葵相互作用的分子方面的综合探索。

IF 3.7 2区生物学

Plant Molecular Biology Pub Date : 2026-07-21 DOI: 10.1007/s11103-026-01736-4

Pooja Parmar, Bishnu Maya Bashyal

{"title":"A comprehensive exploration of molecular aspects of rice-Ustilaginoidea virens interactions.","authors":"Pooja Parmar, Bishnu Maya Bashyal","doi":"10.1007/s11103-026-01736-4","DOIUrl":"10.1007/s11103-026-01736-4","url":null,"abstract":"Rice false smut (RFS) caused by Ustilaginoidea (U.) virens has become an emerging threat to global rice production, driven by complex host-pathogen interactions and stage-specific infection processes. We present a comprehensive review of the omics approach to delve into the interplay of genes and their functional validation in decoding the pathway of infection by U. virens and the resistance mechanism exhibited by rice. Firstly, the pathogenesis genes are discussed, which are triggered at spore germination, progressing to hyphal colonization of floral tissues and smut ball formation. Furthermore, these processes are a consequence of tightly regulated expression of genes of different pathways such as cAMP-MAPK signaling, transcriptional regulators, cell wall integrity factors, virulence determinants, secondary metabolites, and carbohydrate-active enzymes. The rice plants also respond by activating a plethora of defense genes such as phytoalexin biosynthesis, pathogenesis-related proteins, receptor-like kinases, transcription factors, anti-oxidation, etc. The mapping of these genes to related quantitative trait loci and short sequence repeat sequences may aid in gene pyramiding strategies for molecular-assisted breeding programs. The molecular manipulation of resistance genes using CRISPR-Cas9 may further facilitate targeted development of improved rice varieties. Overall, molecular insights of host-pathogen interactions offer decoding of sustainable strategies for developing resilient cultivars and effective management of false smut disease.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"116 4","pages":""},"PeriodicalIF":3.7,"publicationDate":"2026-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148536697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Use of translational fusions to express functional Klebsiella oxytoca dinitrogenase reductase in plant mitochondria. 利用翻译融合体在植物线粒体中表达功能性氧化克雷伯菌二氮酶还原酶。

IF 3.7 2区生物学

Plant Molecular Biology Pub Date : 2026-07-20 DOI: 10.1007/s11103-026-01735-5

Shoko Okada, Xueqin Wang, Christina M Gregg, Robert S Allen, Timothy Rhodes, Vanessa Gillespie, Ingrid Venables, Anu Mathew, Jessica K Bilyj, Keren Byrne, Robert C DeFeyter, Trevor D Rapson, Craig C Wood

{"title":"Use of translational fusions to express functional Klebsiella oxytoca dinitrogenase reductase in plant mitochondria.","authors":"Shoko Okada, Xueqin Wang, Christina M Gregg, Robert S Allen, Timothy Rhodes, Vanessa Gillespie, Ingrid Venables, Anu Mathew, Jessica K Bilyj, Keren Byrne, Robert C DeFeyter, Trevor D Rapson, Craig C Wood","doi":"10.1007/s11103-026-01735-5","DOIUrl":"10.1007/s11103-026-01735-5","url":null,"abstract":"Engineering crop plants with the biological nitrogen fixation pathway is a longstanding goal of modern agriculture. Dinitrogenase reductase (NifH) is a critical component of the biological nitrogen fixation pathway, with multiple roles in metal cofactor assembly and catalysis. This enzyme must be folded correctly as a soluble homodimer and loaded with the [4Fe-4S] metallocluster for function. Previous studies have found that Klebsiella oxytoca (Ko) and Azotobacter vinelandii (Av) NifHs were mostly insoluble when targeted to plant mitochondria. Here we found that a translational fusion of two KoNifH or AvNifH monomers, forming KoNifHH or AvNifHH synthetic dimers, produced a soluble protein when targeted to plant mitochondria and co-expressed with the putative peptidyl-prolyl cis-trans isomerase NifM. KoNifHH isolated after expression in leaf mitochondria at ambient oxygen showed some acetylene reduction activity, which did not require co-expression of the nitrogenase-specific metallocluster machinery NifS and NifU. This activity increased after iron-sulfur cluster reconstitution in vitro with recombinant NifU. In a parallel study, we tested a translational fusion of a variant iron-only dinitrogenase reductase (AvAnfHv6) monomer that was soluble but not active as-isolated from plant mitochondria (AvAnfHHv6) (Gregg et al. 2025a). AvAnfHHv6 was abundant and fully soluble when isolated from plant mitochondria, like its monomer. AvAnfHHv6 was not active as-isolated but could be largely activated by iron-sulfur cluster reconstitution in vitro. This study demonstrates how translational fusions help improve solubility and have the potential to generate an active NifH enzyme within plant mitochondria.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"116 4","pages":""},"PeriodicalIF":3.7,"publicationDate":"2026-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13385145/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148520804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanistic and omics based integrative approaches reveal genetic and molecular basis of drought resilience in sesame (Sesamum indicum L.). 基于机械和组学的综合方法揭示了芝麻抗旱性的遗传和分子基础。

IF 3.7 2区生物学

Plant Molecular Biology Pub Date : 2026-07-13 DOI: 10.1007/s11103-026-01737-3

Sucharita Panda, Gyana Ranjan Rout, Janila Pasupuleti, Lipsa Leena Panigrahi

{"title":"Mechanistic and omics based integrative approaches reveal genetic and molecular basis of drought resilience in sesame (Sesamum indicum L.).","authors":"Sucharita Panda, Gyana Ranjan Rout, Janila Pasupuleti, Lipsa Leena Panigrahi","doi":"10.1007/s11103-026-01737-3","DOIUrl":"10.1007/s11103-026-01737-3","url":null,"abstract":"Sesame (Sesamum indicum L.), is one of the earliest domesticated oilseed crops. It is valued for its exceptional oil content, bioactive compounds, and adaptability to diverse agroclimatic conditions. However, its production remains highly susceptible to water scarcity particularly terminal drought and intermittent moisture deficits that in turn severely compromise the yield and oil quality. Recent advances in genomics, transcriptomics, proteomics, metabolomics, epigenomics, and phenomics have placed sesame as an emerging model for multi-omics-driven stress research. These approaches have uncovered key regulators of drought (NAC, MYB, WRKY), protective proteins (late embryogenesis abundant proteins, heat shock proteins, antioxidant enzymes), osmolyte- and redox-related metabolites, and hormonal signalling modules such as PYL-SnRK2-ABF (ABA), LOX/AOS/OPR (jasmonate), and EIN/ERF (ethylene). Addressing these gaps will require investments in precision phenotyping, robust pan-genomic databases, functional validation using CRISPR/Cas9 tools, and global data-sharing networks. This review highlights the current advances in sesame drought research across omics platforms, critically evaluates their relevance to breeding programs, and offers the first comprehensive multi-omics perspective on moisture-stress adaptation in sesame. Additionally, KEGG pathway-guided multi-omics integration connects ABA signaling, phenylpropanoid metabolism, and antioxidant pathways to drought adaptation in sesame. By bridging mechanistic insights with applied strategies, it highlights pathways to accelerate the development of climate-resilient sesame cultivars.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"116 4","pages":""},"PeriodicalIF":3.7,"publicationDate":"2026-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148431204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tissue specific RISC-loading reassesses small RNA functionality in developing pepper fruit. 组织特异性risc加载重新评估小RNA在发育辣椒果实中的功能。

IF 3.7 2区生物学

Plant Molecular Biology Pub Date : 2026-07-11 DOI: 10.1007/s11103-026-01742-6

Ágnes Dalmadi, Péter Gyula, Auwalu Abdu, András Kis, Fabio Miloro, Jeannette Bálint, György Szittya, Zoltán Havelda

{"title":"Tissue specific RISC-loading reassesses small RNA functionality in developing pepper fruit.","authors":"Ágnes Dalmadi, Péter Gyula, Auwalu Abdu, András Kis, Fabio Miloro, Jeannette Bálint, György Szittya, Zoltán Havelda","doi":"10.1007/s11103-026-01742-6","DOIUrl":"10.1007/s11103-026-01742-6","url":null,"abstract":"Small regulatory RNAs (sRNAs), including small interfering (si) and micro (mi) RNAs, are components of the RNA interference (RNAi) pathways regulating vital processes. These various sRNAs act via specialized executor ARGONAUTE (AGO) proteins constituting RNA induced silencing complexes (RISCs). In Arabidopsis, miRNAs load mainly into AGO1, while 24-nucleotide (nt) long siRNAs load into AGO4, typically. However, a large subset of sRNAs is present in the cytoplasm in AGO-unbound form. To uncover the potential importance of tissue specific AGO-loading efficiency of sRNAs in an economically important plant we used pepper fruits, dissected into pericarp, placenta, and seed and the ratio between AGO-loaded and -unloaded sRNA pools was investigated with high throughput sequencing. In agreement with the previous studies, variable loading capacity of sRNAs was discovered with an abundant AGO-unbound pool. The investigated pools showed a surprisingly high prevalence of 21-22-24-nt long siRNAs even in the high molecular weight RISC. The dynamically expanding pericarp exhibited extremely high 21-nt long miRNA content. Moreover, comparing the different tissues, a drastic redistribution or elimination of 21- and 24-nt sRNAs in various sRNA-protein complexes and changes in unbound sRNA pools were observed demonstrating the highly dynamic alteration in AGO-loading properties of the sRNAs. These data imply that during fruit development sRNAs are dynamically sorted into various executor complexes in a controlled manner. This finding also implies that analysis of the content of AGO-sRNA complexes together with the AGO-unbound population of these sRNAs can be a useful tool to identify potentially functional sRNAs.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"116 4","pages":""},"PeriodicalIF":3.7,"publicationDate":"2026-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13356050/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148423185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heavy fruit load inhibits axillary bud outgrowth initially through weakening its sink strength and auxin efflux in citrus. 重果负荷最初通过削弱腋芽的汇强度和生长素外排抑制腋芽的生长。

IF 3.7 2区生物学

Plant Molecular Biology Pub Date : 2026-07-10 DOI: 10.1007/s11103-026-01743-5

Yin Luo, Ling-Li Yang, Ying-Qi Xiong, Yong-Zhong Liu, Shariq Mahmood Alam, Abdelmonem Elshahat, Sami Ur Rehman, Guo-Guo Li

{"title":"Heavy fruit load inhibits axillary bud outgrowth initially through weakening its sink strength and auxin efflux in citrus.","authors":"Yin Luo, Ling-Li Yang, Ying-Qi Xiong, Yong-Zhong Liu, Shariq Mahmood Alam, Abdelmonem Elshahat, Sami Ur Rehman, Guo-Guo Li","doi":"10.1007/s11103-026-01743-5","DOIUrl":"10.1007/s11103-026-01743-5","url":null,"abstract":"Excessive and random outgrowth of axillary buds (AxBs) produce a mass of summer shoots, resulting in overly tall and dense canopies that complicate disease and pest control. Heavy fruit load reduces the number of summer shoots. However, the underlying molecular mechanism for this suppression remains unclear. In this study, we found that transcript levels of two well-characterized dormancy marker genes (CsBRC1 and CsDRM1) were significantly higher in AxBs from fruit-bearing branchlet (FB) than from vegetative branchlet (VB). However, de-fruiting significantly decreased them within 9 days. Transcriptome analysis identified 5638 differentially expressed genes (DEGs) after de-fruiting, many of which are associated with sugar and phytohormone homeostasis. Interestingly, transcript levels of sink strength-related genes (CsCwINV6 and CsSUS6) and auxin-efflux carrier gene CsPIN1a were significantly increased within 2 h after de-fruiting. Furthermore, their expression was markedly lower in FB AxBs than in VB AxBs and was significantly induced by sucrose supplementation. Collectively, these results suggested that a heavy fruit load suppresses the expression of sink strength-associated genes and CsPIN1a, thereby reducing sugar availability and impairing auxin export from axillary buds, ultimately inhibiting the outgrowth of AxBs. These findings provide valuable insights into the molecular basis of poor vegetative growth under heavy fruit load and contribute to simplifying canopy management through rationalizing fruit load.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"116 4","pages":""},"PeriodicalIF":3.7,"publicationDate":"2026-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148423125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic basis of alkaloid divergence in the Solanaceae. 茄科生物碱分化的遗传基础。

IF 3.7 2区生物学

Plant Molecular Biology Pub Date : 2026-06-12 DOI: 10.1007/s11103-026-01718-6

Pablo A Pérez-Mesa, Ronald A Pardo, Saleh Alseekh, Sofía Rojas-Contreras, Gina P Sierra, D Estevan García, Andrés Orejuela, Liliana Lopez-Kleine, Alisdair R Fernie, Federico Roda

{"title":"Genetic basis of alkaloid divergence in the Solanaceae.","authors":"Pablo A Pérez-Mesa, Ronald A Pardo, Saleh Alseekh, Sofía Rojas-Contreras, Gina P Sierra, D Estevan García, Andrés Orejuela, Liliana Lopez-Kleine, Alisdair R Fernie, Federico Roda","doi":"10.1007/s11103-026-01718-6","DOIUrl":"10.1007/s11103-026-01718-6","url":null,"abstract":"Plant lineages produce distinct alkaloids, indicating the presence of evolutionary mechanisms that prevent the simultaneous metabolism of multiple alkaloid types within the same species. In the Solanaceae family, species produce either tropane alkaloids (TAs) or steroidal glycoalkaloids (SGAs). To investigate the genetic causes underlying this mutually exclusive distribution, we integrated genomic, transcriptomic, and metabolomic data across tens of species representing most Solanaceae clades, focusing on three potential mechanisms: (1) loss of biosynthetic genes, (2) genomic clustering, and (3) regulatory changes. Ancestral trait reconstruction suggests that both pathways were likely present in early Solanaceae lineages but were differentially lost across clades, resulting in transitions between SGA and TA biosynthesis. Notably, SGA and TA genes exhibit contrasting patterns of genomic distribution: SGA genes are organized in large, dynamic biosynthetic clusters, while TA genes are dispersed across the genome. More importantly, SGA genes are ancient and phylogenetically widespread, but are not expressed in clades that produce TAs, implying that a regulatory switch was responsible for the simultaneous silencing of SGA genes in these clades. Finally, we observed the loss and genetic erosion of late-acting SGA and TA genes in clades where they are not expressed. Overall, our study suggests that genomic clustering, regulatory changes, and gene duplication/loss underlie the mutually exclusive distribution of alkaloids in the Solanaceae.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"116 4","pages":""},"PeriodicalIF":3.7,"publicationDate":"2026-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148227446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrative approach to identify robust pattern recognition receptors in Eucalyptus grandis: novel candidates for disease resistance. 鉴定大桉树稳健模式识别受体的综合方法：抗病的新候选者。

IF 3.7 2区生物学

Plant Molecular Biology Pub Date : 2026-06-11 DOI: 10.1007/s11103-026-01734-6

Y E Gonzalez, N C Aguirre, C V Filippi

{"title":"Integrative approach to identify robust pattern recognition receptors in Eucalyptus grandis: novel candidates for disease resistance.","authors":"Y E Gonzalez, N C Aguirre, C V Filippi","doi":"10.1007/s11103-026-01734-6","DOIUrl":"10.1007/s11103-026-01734-6","url":null,"abstract":"Pattern-recognition receptors (PRRs) initiate plant pattern-triggered immunity (PTI), encompassing receptor-like kinases (RLKs) and receptor-like proteins (RLPs). Eucalyptus grandis, an economically important species worldwide, is a long-lived organism that faces multiple disease pressures. The deployment of PRR-based breeding tools offers a route to broad, non-race-specific resistance that can remain effective across outbreaks. The objective of this work was to identify and characterise the PRR repertoire of E. grandis using public multi-omics data. Two state-of-the-art predictors were compared with a multi-species curation and a meta-analysis was conducted compiling transcriptomic data under biotic stress. A set of 730 RLKs/RLPs (PRR candidates) were consistently identified across three sources (~ 1.6% of protein-coding genes), of which 283 were differentially expressed (RLKs/RLPs-DEG). A PFAM domain-based scheme was applied to standardise classification across tools. RLKs (TM-K-LRR) predominated over RLPs (TM-LRR), while many carried malectin or malectin-like ectodomains. RLKs/RLPs genes were unevenly distributed across the genome: chromosome 6 had the highest count and the densest clustering. Gene-family expansion appeared mainly driven by duplication, with extensive tandem arrays supported by segmental, proximal and dispersed events. Gene Ontology and cis-elements annotations in RLKs/RLPs-DEG/no-DEG showed significant enrichment in terms related to cell cycle development, hormonal regulation and stress response. We proposed a catalogue of 16 RLKs which resulted in DEGs against at least three pathogens, suggesting their broad spectrum and robustness. Most of them presented orthologues with cross-taxon evidence of defence roles. This study delineates novel multi-pathogen candidate PRR genes, providing valuable information to assist Eucalyptus breeding programs.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"116 4","pages":""},"PeriodicalIF":3.7,"publicationDate":"2026-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148219168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Splicing regulation by RS2Z36 controls ovary patterning and fruit growth in tomato. RS2Z36的剪接调控控制着番茄子房形态和果实生长。

IF 3.7 2区生物学

Plant Molecular Biology Pub Date : 2026-06-09 DOI: 10.1007/s11103-026-01730-w

Stavros Vraggalas, Remus R E Rosenkranz, Mario Keller, Yolanda Pérez-Pérez, Samia Bachiri, Kerstin J Zehl, Jessica Bold, Stefan Simm, Arindam Ghatak, Wolfram Weckwerth, Leila Afjehi-Sadat, Palak Chaturvedi, Pilar S Testillano, Michaela Müller-McNicoll, Kathi Zarnack, Sotirios Fragkostefanakis

{"title":"Splicing regulation by RS2Z36 controls ovary patterning and fruit growth in tomato.","authors":"Stavros Vraggalas, Remus R E Rosenkranz, Mario Keller, Yolanda Pérez-Pérez, Samia Bachiri, Kerstin J Zehl, Jessica Bold, Stefan Simm, Arindam Ghatak, Wolfram Weckwerth, Leila Afjehi-Sadat, Palak Chaturvedi, Pilar S Testillano, Michaela Müller-McNicoll, Kathi Zarnack, Sotirios Fragkostefanakis","doi":"10.1007/s11103-026-01730-w","DOIUrl":"10.1007/s11103-026-01730-w","url":null,"abstract":"Fruit growth is mediated by cell division and expansion. In tomato, the model for fleshy fruit development, both processes are tightly linked to changes in gene expression, including transcriptional regulation and RNA processing. While several transcription factors are implicated in fruit developmental programs, the role of splicing regulators remains largely unexplored. Expression profiling of splicing-related genes revealed expression patterns. The serine/arginine-rich splicing factor RS2Z36 is expressed in ovaries and during early fruit development. Loss-of-function mutations in RS2Z36 result in ovaries with altered patterning and in smaller, ellipsoid fruits. rs2z36 mutants display elongated pericarp cells along the longitudinal axis of pre-anthesis ovaries, indicating that RS2Z36-dependent expansion patterns are established before anthesis. Based on RNA-seq analysis we identified 230 genes with altered splicing profile in ovaries of rs2z36.1 compared to WT and 235 differentially expressed genes. Proteome analysis further revealed several differentially abundant isoforms, including several cell wall proteins and modifiers that might be involved in ovary patterning and fruit growth. In addition, rs2z36-1 pericarps show increased deposition of LM6-recognized arabinan and AGP epitopes. Together, these findings identify RS2Z36 as a regulator of ovary and fruit development and highlight a previously underappreciated role for splicing control in shaping early fruit morphology.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"116 4","pages":""},"PeriodicalIF":3.7,"publicationDate":"2026-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13249928/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148212342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0