{"title":"豌豆抗枯萎病的遗传与分子研究进展及展望。","authors":"Neha Verma, Saurabh Yadav, Neha Rana, Rishabh Maheshwari, Manpreet Kaur, Priyanka Kumari, Parteek Kumar, Rajinder Kumar Dhall, Hira Singh, Priti Sharma, Parveen Chunneja","doi":"10.1007/s11103-025-01624-3","DOIUrl":null,"url":null,"abstract":"<p><p>Garden pea is an important leguminous crop valued for its protein-rich food source for human nutrition and enhancing agricultural sustainability by fixing nitrogen biologically. However, its cultivation faces significant challenges from pests, diseases, and environmental stresses. Among these, Fusarium wilt (FW) caused by pathogen Fusarium oxysporum f. sp. pisi poses a severe threat, resulting in substantial yield losses globally. Four pathogenic races (1, 2, 5, and 6) of this fungus have been primarily identified, and its broad host range further complicates effective management. Traditional control methods including cultural practices, physical control, biological interventions, and chemical treatments have shown limited efficacy. Consequently, host-plant resistance has emerged as a sustainable and practical solution for managing FW. In this review, the advancements in genetics with modern molecular techniques such as SNP genotyping, QTL mapping, and marker-assisted selection for the development of FW resistant pea varieties were highlighted. Furthermore, we also discussed the omics techniques viz., transcriptomics, metabolomics and proteomics and innovative breeding techniques like CRISPR-mediated genome editing, speed breeding, and genomic selection for revolutionize FW resistance breeding programs in pea. Therefore, this review focuses on integrating cutting-edge molecular techniques with omics approaches to unravel Fusarium wilt defense mechanisms in garden pea, aiming to accelerate genetic gains and develop superior disease-resistant varieties for improved productivity and quality.</p>","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"115 4","pages":"89"},"PeriodicalIF":3.8000,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Genetic and molecular approaches for Fusarium wilt resistance in garden pea: advances and future outlook.\",\"authors\":\"Neha Verma, Saurabh Yadav, Neha Rana, Rishabh Maheshwari, Manpreet Kaur, Priyanka Kumari, Parteek Kumar, Rajinder Kumar Dhall, Hira Singh, Priti Sharma, Parveen Chunneja\",\"doi\":\"10.1007/s11103-025-01624-3\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Garden pea is an important leguminous crop valued for its protein-rich food source for human nutrition and enhancing agricultural sustainability by fixing nitrogen biologically. However, its cultivation faces significant challenges from pests, diseases, and environmental stresses. Among these, Fusarium wilt (FW) caused by pathogen Fusarium oxysporum f. sp. pisi poses a severe threat, resulting in substantial yield losses globally. Four pathogenic races (1, 2, 5, and 6) of this fungus have been primarily identified, and its broad host range further complicates effective management. Traditional control methods including cultural practices, physical control, biological interventions, and chemical treatments have shown limited efficacy. Consequently, host-plant resistance has emerged as a sustainable and practical solution for managing FW. In this review, the advancements in genetics with modern molecular techniques such as SNP genotyping, QTL mapping, and marker-assisted selection for the development of FW resistant pea varieties were highlighted. Furthermore, we also discussed the omics techniques viz., transcriptomics, metabolomics and proteomics and innovative breeding techniques like CRISPR-mediated genome editing, speed breeding, and genomic selection for revolutionize FW resistance breeding programs in pea. 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引用次数: 0
摘要
豌豆是一种重要的豆科作物,其富含蛋白质的食物来源对人类营养和通过生物固氮提高农业可持续性具有重要价值。然而,它的种植面临着病虫害和环境压力的重大挑战。其中,由尖孢镰刀菌(Fusarium oxysporum f. sp. pisi)引起的枯萎病(Fusarium wilt, FW)在全球范围内造成了严重的产量损失。该真菌的四个致病小种(1、2、5和6)已被初步鉴定,其广泛的寄主范围进一步使有效管理复杂化。传统的控制方法,包括文化实践、物理控制、生物干预和化学处理,显示出有限的效果。因此,寄主植物抗性已成为管理FW的可持续和实用的解决方案。本文综述了利用现代分子技术,如SNP基因分型、QTL定位、标记辅助选择等在豌豆抗FW品种开发中的研究进展。此外,我们还讨论了组学技术,即转录组学、代谢组学和蛋白质组学,以及crispr介导的基因组编辑、快速育种和基因组选择等创新育种技术,以彻底改变豌豆的FW抗性育种计划。因此,本文将结合前沿分子技术和组学方法,揭示豌豆枯萎病的防御机制,旨在加快遗传增益,开发出优质抗病品种,提高产量和品质。
Genetic and molecular approaches for Fusarium wilt resistance in garden pea: advances and future outlook.
Garden pea is an important leguminous crop valued for its protein-rich food source for human nutrition and enhancing agricultural sustainability by fixing nitrogen biologically. However, its cultivation faces significant challenges from pests, diseases, and environmental stresses. Among these, Fusarium wilt (FW) caused by pathogen Fusarium oxysporum f. sp. pisi poses a severe threat, resulting in substantial yield losses globally. Four pathogenic races (1, 2, 5, and 6) of this fungus have been primarily identified, and its broad host range further complicates effective management. Traditional control methods including cultural practices, physical control, biological interventions, and chemical treatments have shown limited efficacy. Consequently, host-plant resistance has emerged as a sustainable and practical solution for managing FW. In this review, the advancements in genetics with modern molecular techniques such as SNP genotyping, QTL mapping, and marker-assisted selection for the development of FW resistant pea varieties were highlighted. Furthermore, we also discussed the omics techniques viz., transcriptomics, metabolomics and proteomics and innovative breeding techniques like CRISPR-mediated genome editing, speed breeding, and genomic selection for revolutionize FW resistance breeding programs in pea. Therefore, this review focuses on integrating cutting-edge molecular techniques with omics approaches to unravel Fusarium wilt defense mechanisms in garden pea, aiming to accelerate genetic gains and develop superior disease-resistant varieties for improved productivity and quality.
期刊介绍:
Plant Molecular Biology is an international journal dedicated to rapid publication of original research articles in all areas of plant biology.The Editorial Board welcomes full-length manuscripts that address important biological problems of broad interest, including research in comparative genomics, functional genomics, proteomics, bioinformatics, computational biology, biochemical and regulatory networks, and biotechnology. Because space in the journal is limited, however, preference is given to publication of results that provide significant new insights into biological problems and that advance the understanding of structure, function, mechanisms, or regulation. Authors must ensure that results are of high quality and that manuscripts are written for a broad plant science audience.