Plant Molecular Biology最新文献

Chemical application improves stress resilience in plants.

IF 3.9 2区生物学

Plant Molecular Biology Pub Date : 2025-03-19 DOI: 10.1007/s11103-025-01566-w

Khurram Bashir, Daisuke Todaka, Kaori Sako, Minoru Ueda, Farhan Aziz, Motoaki Seki

{"title":"Chemical application improves stress resilience in plants.","authors":"Khurram Bashir, Daisuke Todaka, Kaori Sako, Minoru Ueda, Farhan Aziz, Motoaki Seki","doi":"10.1007/s11103-025-01566-w","DOIUrl":"https://doi.org/10.1007/s11103-025-01566-w","url":null,"abstract":"In recent years, abiotic stresses, including droughts, floods, high temperatures, and salinity, have become increasingly frequent and severe. These stresses significantly hinder crop yields and product quality, posing substantial challenges to sustainable agriculture and global food security. Simultaneously, the rapidly growing global population exacerbates the need to enhance crop production under worsening environmental conditions. Consequently, the development of effective strategies to strengthen the resilience of crop plants against high temperatures, water scarcity, and extreme environmental conditions is critical for mitigating the impacts of abiotic stress. Plants respond to these environmental challenges by reprogramming their transcriptome and metabolome. Common strategies for developing stress-tolerant plants include screening germplasm, generating transgenic crop plants, and employing genome editing techniques. Recently, chemical treatment has emerged as a promising approach to enhance abiotic stress tolerance in crops. This technique involves the application of exogenous chemical compounds that induce molecular and physiological changes, thereby providing a protective shield against abiotic stress. Forward and reverse genetic approaches have facilitated the identification of chemicals capable of modulating plant responses to abiotic stresses. These priming agents function as epigenetic regulators, agonists, or antagonists, playing essential roles in regulating stomatal closure to conserve water, managing cellular signaling through reactive oxygen species and metabolites to sustain plant growth, and activating gluconeogenesis to enhance cellular metabolism. This review summarizes recent advancements in the field of chemical priming and explores strategies to improve stress tolerance and crop productivity, thereby contributing to the enhancement of global food security.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"115 2","pages":"47"},"PeriodicalIF":3.9,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143664139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chemically-induced cellular stress signals are transmitted to alternative splicing via UsnRNA levels to alter gene expression in Arabidopsis thaliana.

IF 3.9 2区生物学

Plant Molecular Biology Pub Date : 2025-03-16 DOI: 10.1007/s11103-025-01575-9

Hirokazu Takahashi, Toshihiro Arae, Kodai Ishibashi, Ryosuke Sano, Taku Demura, Misato Ohtani

{"title":"Chemically-induced cellular stress signals are transmitted to alternative splicing via UsnRNA levels to alter gene expression in Arabidopsis thaliana.","authors":"Hirokazu Takahashi, Toshihiro Arae, Kodai Ishibashi, Ryosuke Sano, Taku Demura, Misato Ohtani","doi":"10.1007/s11103-025-01575-9","DOIUrl":"10.1007/s11103-025-01575-9","url":null,"abstract":"Alternative pre-mRNA splicing (AS) is a crucial regulatory layer of gene expression in eukaryotes. AS patterns can change in response to abiotic and biotic stress, allowing cellular functions to adapt to environmental conditions. Here, we examined the effects of cellular stress-inducing chemicals on AS-mediated gene regulation in Arabidopsis thaliana by investigating the alternatively spliced forms of SERINE-ARGININE PROTEIN30 (SRp30) and U1-70 K, encoding splicing factors, as well as ASCORBATE PEROXIDASE3 (APX3) and FOLYLPOLYGLUTAMATE SYNTHASE3 (FPGS3), encoding enzymes important for stress responses. Disrupting key cellular activities, including nitric oxide metabolism, ATPase activity, plastid function, and genome stability, affected AS patterns in Arabidopsis. Stress treatment altered the abundance of uridine-rich small nuclear RNAs (UsnRNAs), especially U1 snRNAs, which are essential non-coding RNA components of U1 small nuclear ribonucleoproteins (U1 snRNPs), suggesting that abnormalities in AS are partially mediated by changes in U1 snRNA levels. The shoot redifferentiation defectice2-1 (srd2-1) mutant defective for snRNA transcription was hypersensitive for stress treatment, since it showed changes in AS patterns at lower concentrations of stress inducers to compare with the wild type. Together, our data suggest that cellular stress can influence gene expression in plants by regulating AS, which is partially regulated by UsnRNA levels through the SRD2-mediated snRNA transcription.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"115 2","pages":"46"},"PeriodicalIF":3.9,"publicationDate":"2025-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11911268/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143639779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A glycogen synthase kinase-3 gene enhances grain yield heterosis in semi-dwarf rapeseed. 糖原合酶激酶-3基因可提高半矮油菜籽的籽粒产量异质性。

IF 3.9 2区生物学

Plant Molecular Biology Pub Date : 2025-03-14 DOI: 10.1007/s11103-025-01555-z

Li Bao, Liu Xinhong, Yang Qian, Zhang Hui, Tan Wenqing, Yan Mingli, Deng Lichao, Li Mei, Qu Liang, Guo Yiming

{"title":"A glycogen synthase kinase-3 gene enhances grain yield heterosis in semi-dwarf rapeseed.","authors":"Li Bao, Liu Xinhong, Yang Qian, Zhang Hui, Tan Wenqing, Yan Mingli, Deng Lichao, Li Mei, Qu Liang, Guo Yiming","doi":"10.1007/s11103-025-01555-z","DOIUrl":"https://doi.org/10.1007/s11103-025-01555-z","url":null,"abstract":"Optimizing plant height is a key breeding objective in Brassica napus to enhance lodging resistance and increase yield potential. In the present study, we identified a semi-dwarf gene in rapeseed, BnDWARF5 (BnDF5), which encodes a glycogen synthase kinase 3, BRASSINOSTEROID-INSENSITIVE 2 (BnaC03.BIN2), primarily controlling the elongation of basal internodes by inhibiting the elongation of internode cells. Genetic mapping and cloning revealed that BnDF5 is governed by a semi-dominant/dominant gene located on chromosome C03. Sequencing uncovered an SNP in BnaC03.BIN2 due to an amino acid substitution, which was confirmed via kompetitive allele-specific polymerase chain reaction marker analysis, and expressing the mutated BnaC03.BIN2 in the wild type resulted in decreased plant height. Practical breeding applications showed that heterozygous BnDF5 plants exhibited optimal intermediate height and strong yield heterosis, making the semi-dwarf mutant a valuable genetic resource for developing semi-dwarf rapeseed varieties with improved lodging resistance and yield.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"115 2","pages":"45"},"PeriodicalIF":3.9,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143630876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Light regulates seed dormancy through FHY3-mediated activation of ACC OXIDASE 1 in Arabidopsis.

IF 3.9 2区生物学

Plant Molecular Biology Pub Date : 2025-03-13 DOI: 10.1007/s11103-025-01559-9

Yitong Liu, Shuangrong Liu, Yanjun Jing, Jialong Li, Rongcheng Lin

{"title":"Light regulates seed dormancy through FHY3-mediated activation of ACC OXIDASE 1 in Arabidopsis.","authors":"Yitong Liu, Shuangrong Liu, Yanjun Jing, Jialong Li, Rongcheng Lin","doi":"10.1007/s11103-025-01559-9","DOIUrl":"https://doi.org/10.1007/s11103-025-01559-9","url":null,"abstract":"Seed dormancy enables plants to delay germination until conditions are favorable for the survival of the next generation. Seed dormancy and germination are controlled by a combination of external and internal signals, in which light and ethylene act as critical regulators. However, how light and ethylene are interlinked to control these two processes remains to be investigated. Here, we show that ethylene and its precursor, 1-aminocyclopropane-1-carboxylic acid (ACC), promote seed germination under light. Light facilitates the conversion of ACC to ethylene, in which phytochrome B (phyB) and FAR-RED ELONGATED HYPOCOTYL3 (FHY3) are functionally required. ACC oxidases (ACOs) catalyze the conversion of ACC to ethylene, among which ACO1 is specifically and predominantly expressed in imbibed seeds. Ethylene induces FHY3 protein accumulation in imbibed seeds, whereby FHY3 directly binds to ACO1 promoter and specifically mediates light-promoted ACO1 expression. Light promotes ACO1 protein accumulation. Overexpression of ACO1 significantly promotes seed germination, and almost completely restores the dormant defect of fhy3 loss-of-function mutants. In summary, this study reveals an ethylene-responsive regulatory cascade of phyB-FHY3-ACO1 that integrates external light input with internal factors to regulate seed dormancy and germination.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"115 2","pages":"44"},"PeriodicalIF":3.9,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143625620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the drought-responsive miRNAs and their corresponding target genes in chickpea root tissue.

IF 3.9 2区生物学

Plant Molecular Biology Pub Date : 2025-03-12 DOI: 10.1007/s11103-025-01572-y

Lalbahadur Singh, Deshika Kohli, Yashwant K Yadava, Sheel Yadav, Kishor Gaikwad, Chellapilla Bharadwaj, Pradeep Kumar Jain

{"title":"Exploring the drought-responsive miRNAs and their corresponding target genes in chickpea root tissue.","authors":"Lalbahadur Singh, Deshika Kohli, Yashwant K Yadava, Sheel Yadav, Kishor Gaikwad, Chellapilla Bharadwaj, Pradeep Kumar Jain","doi":"10.1007/s11103-025-01572-y","DOIUrl":"https://doi.org/10.1007/s11103-025-01572-y","url":null,"abstract":"Chickpea is an important pulse crop globally, with major production in Southeast Asia. However, the production of chickpea is hampered due to various biotic and abiotic stressors. In response to such stressors, microRNAs which are small non-coding regulatory RNA molecules have been observed as key players. The present study evaluates the role of drought-responsive microRNAs in the root tissues of chickpea genotypes contrasting for drought tolerance. This study led to the generation of 146.7 million short-read sequences from small RNA libraries constructed from the root tissues of the two genotypes. Upon analysis, 224 conserved and 155 novel miRNA sequences were identified. The miR156 family was found to be the most abundant among the 51 families identified for the conserved miRNAs. Quantitative real-time PCR (qRT-PCR) was used to validate eleven conserved and six novel miRNAs. The identification of drought-induced expression of specific miRNAs and their related target genes suggests miRNA-mediated response mechanisms in chickpea. Furthermore, this research investigated the role of drought-responsive miRNAs, specifically miR171 and miR166 and their target genes, SCL27 (scarecrow-like protein 27) and ATHB15 (Homeobox-leucine zipper family protein), respectively. The study validated the miR171 and miR166 directed cleavage of SCL27 and ATHB15, respectively, in drought-stressed root tissues using 5´RLM-RACE (5' RNA Ligase-Mediated Rapid Amplification of cDNA Ends) analysis. The study highlights the role of diverse miRNAs in chickpea for mitigating drought.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"115 2","pages":"43"},"PeriodicalIF":3.9,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143616780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inherited endurance: deciphering genetic associations of transgenerational and intergenerational heat stress memory in barley.

IF 3.9 2区生物学

Plant Molecular Biology Pub Date : 2025-03-10 DOI: 10.1007/s11103-025-01571-z

Amr Elkelish, Ahmad M Alqudah, Abdulrahman M Alhudhaibi, Hussain Alqahtani, Andreas Börner, Samar G Thabet

{"title":"Inherited endurance: deciphering genetic associations of transgenerational and intergenerational heat stress memory in barley.","authors":"Amr Elkelish, Ahmad M Alqudah, Abdulrahman M Alhudhaibi, Hussain Alqahtani, Andreas Börner, Samar G Thabet","doi":"10.1007/s11103-025-01571-z","DOIUrl":"https://doi.org/10.1007/s11103-025-01571-z","url":null,"abstract":"Barley (Hordeum vulgare L.), a cornerstone of global cereal crops, is increasingly vulnerable to concurrent heat stress, a critical abiotic factor that is intensified by climate change. This study employed genome-wide association studies (GWAS) to investigate \"stress memory,\" a phenomenon where prior stress exposure enhances a plant's response to subsequent stress events. In this study, we analyzed essential agronomic traits, including plant height, spike length, grain number, and thousand-kernel weight, in conjunction with biochemical markers such as chlorophyll content, proline, and soluble proteins. These assessments spanned three successive generations under heat stress, capturing transgenerational and intergenerational effects and uncovering the cumulative impacts of prolonged stress in the third generation. Markedly, our findings highlight the critical influence of heat stress on plant physiology across generational scales, showcasing significant reductions in chlorophyll content, which reflect stress-induced limitations on photosynthetic capacity. In contrast, the observed consistent and substantial increases in proline and soluble protein content across transgenerational, intergenerational, and third-generation stress memory stages underscore their vital roles in stress mitigation and cellular homeostasis. These results provide compelling evidence of generational stress memory, suggesting potential adaptive strategies that plants employ to cope with harsh environmental conditions. Interestingly, identifying significant SNP markers within key genomic regions using GWAS analysis further highlights the potential for harnessing these loci in breeding programs. These results shed light on the intricate mechanisms of barley's stress tolerance and underscore the potential of integrating genomic, epigenomic, and advanced phenotyping tools into breeding programs to develop heat-resilient cultivars.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"115 2","pages":"42"},"PeriodicalIF":3.9,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143597560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing wheat resilience: biotechnological advances in combating heat stress and environmental challenges.

IF 3.9 2区生物学

Plant Molecular Biology Pub Date : 2025-03-09 DOI: 10.1007/s11103-025-01569-7

Muhammad Arif, Muhammad Haroon, Ayesha Fazal Nawaz, Hina Abbas, Ruhong Xu, Luhua Li

{"title":"Enhancing wheat resilience: biotechnological advances in combating heat stress and environmental challenges.","authors":"Muhammad Arif, Muhammad Haroon, Ayesha Fazal Nawaz, Hina Abbas, Ruhong Xu, Luhua Li","doi":"10.1007/s11103-025-01569-7","DOIUrl":"https://doi.org/10.1007/s11103-025-01569-7","url":null,"abstract":"Climate change, with its increasing temperatures, is significantly disrupting global agricultural systems, and wheat, a key cereal crop faces severe challenges. Heat stress has emerged as a critical threat, accelerating wheat growth, leading to premature maturation, reduced grain filling, and ultimately lower yields. The situation is exacerbated by more frequent and intense heat waves, particularly in regions already struggling with water scarcity. Maintaining the delicate balance of temperature and water necessary for optimal wheat production is becoming challenging, posing a serious risk to global food security. Therefore, there is an urgent need to develop adaptive strategies with innovations in breeding and transgenic technologies crucial to improving wheat resilience to environmental stresses, especially to combat the growing impacts of heat stress. Modern tools like CRISPR/Cas9, Transcription Activator-Like Effector Nucleases, and Zinc Finger Nucleases have been instrumental in developing wheat varieties with improved traits. However, the future of wheat cultivation requires more than just resistance to a single stressor. As climate change intensifies, there is an urgent need for wheat varieties that can withstand multiple stresses, including heat, drought, and pests. Developing these multi-stress-tolerant cultivars is crucial for ensuring food security in a rapidly changing climate.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"115 2","pages":"41"},"PeriodicalIF":3.9,"publicationDate":"2025-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143586684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Disruption of the endoplasmic reticulum-localized fatty acyl-ACP thioesterase IPF1 caused partial male sterility in rice.

IF 3.9 2区生物学

Plant Molecular Biology Pub Date : 2025-03-08 DOI: 10.1007/s11103-025-01574-w

Wenye Tan, Jingfei Tian, Wenfeng Zhao, Jianxin Wei, Yibo Xu, Shixu Zhou, Zihan Wei, Zejun Shen, Minghang Wu, Lianguang Shang, Rongbai Li, Yongfei Wang, Baoxiang Qin

{"title":"Disruption of the endoplasmic reticulum-localized fatty acyl-ACP thioesterase IPF1 caused partial male sterility in rice.","authors":"Wenye Tan, Jingfei Tian, Wenfeng Zhao, Jianxin Wei, Yibo Xu, Shixu Zhou, Zihan Wei, Zejun Shen, Minghang Wu, Lianguang Shang, Rongbai Li, Yongfei Wang, Baoxiang Qin","doi":"10.1007/s11103-025-01574-w","DOIUrl":"10.1007/s11103-025-01574-w","url":null,"abstract":"The fatty acyl ACP thioesterases, catalyzing the final step of fatty acid synthesis in the plastid, regulate various critical processes in plants, including seed oil accumulation, seed development, plant growth, and drought tolerance. However, their roles in male fertility have seldom been demonstrated. In this study, the function of a newly FAT, Impaired Pollen Fertility 1 (IPF1) in male fertility was investigated. IPF1 expressed prominently in microspores and tapetum. IPF1 specifically located in the endoplasmic reticulum. IPF1 knock-out mutants produced by the CRISPR/Cas9 system displayed significant reduction in seed-setting rate compared to WT. The decreased seed-setting rate in the ipf1 mutants was found to be attributed to the defects of pollen viability, not the female gamete fertility. The aborted pollen in the ipf1 mutants showed impaired pollen wall formation and diminished lipid deposition. Consistently, the expression levels of six genes critical to pollen wall formation and lipid metabolism (GPAT3, OsC6, DPW2, OsPKS1, OsPKS2, and OsSTRL2) were significantly decreased in the ipf1 mutant. Taken together, these results demonstrate that IPF1 regulates rice pollen fertility through the modulation of lipid synthesis.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"115 2","pages":"40"},"PeriodicalIF":3.9,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143582348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phenylpropanoids for the control of fungal diseases of postharvest fruit. 用于控制采后水果真菌病害的苯基丙酮。

IF 3.9 2区生物学

Plant Molecular Biology Pub Date : 2025-02-28 DOI: 10.1007/s11103-025-01568-8

Yijie Sun, Xiaohan Wang, Zhengyu Huang, Xiaoyang Zhao, Linxiang Qiao, Caie Wu, Zhaohui Xue, Xiaohong Kou

{"title":"Phenylpropanoids for the control of fungal diseases of postharvest fruit.","authors":"Yijie Sun, Xiaohan Wang, Zhengyu Huang, Xiaoyang Zhao, Linxiang Qiao, Caie Wu, Zhaohui Xue, Xiaohong Kou","doi":"10.1007/s11103-025-01568-8","DOIUrl":"https://doi.org/10.1007/s11103-025-01568-8","url":null,"abstract":"In recent years, there has been a growing interest in developing greener and safer substances for the control of postharvest fungal diseases of fruit. Secondary metabolic pathways play an important role in plant defense responses, and the phenylpropanoid metabolic pathway is one of the most important secondary metabolic pathways in plant defense. More and more studies have shown that exogenous phenylpropanoids treatments can inhibit postharvest fungal diseases. On the one hand, these biologically active phenylpropanoids are fungistatic and can act directly on the fungal cells infesting the postharvest fruit to inhibit spore germination and mycelial growth. On the other hand, phenylpropanoids treatment can improve plant resistance. In this review, we summarize recent achievements in the mechanisms and applications of phenylpropanoids, including cinnamic acid, p-coumaric acid and esters, caffeic acid, ferulic acid, and chlorogenic acid, in the inhibition of fungal pathogens and the reduction of postharvest losses. In addition, we propose further research hotspots and development directions based on combining nanomaterials and biotechnology.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"115 2","pages":"39"},"PeriodicalIF":3.9,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143531116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The involvement of auxin response factor OsARF7 in positively regulating root development by mediating the expression of OsCRL1 in rice (Oryza sativa L.).

IF 3.9 2区生物学

Plant Molecular Biology Pub Date : 2025-02-26 DOI: 10.1007/s11103-025-01570-0

Congying Sun, Kai Fan, Xin Wang, Honghai Liu, Nuoping Guo, Wanyu Liu, Guixiang Ye, Weiwei Lin, Wenxiong Lin, Zhaowei Li

{"title":"The involvement of auxin response factor OsARF7 in positively regulating root development by mediating the expression of OsCRL1 in rice (Oryza sativa L.).","authors":"Congying Sun, Kai Fan, Xin Wang, Honghai Liu, Nuoping Guo, Wanyu Liu, Guixiang Ye, Weiwei Lin, Wenxiong Lin, Zhaowei Li","doi":"10.1007/s11103-025-01570-0","DOIUrl":"https://doi.org/10.1007/s11103-025-01570-0","url":null,"abstract":"The root is one of the most important organs that determines the final grain yield in rice. Auxin is essential for root development in plants. Rice auxin response factor7 (OsARF7), belonging to the ARF family, is a key regulator of root development. Here, we show that OsARF7 positively regulates root development via auxin signaling. The osarf7 mutants display a significant decrease in the root number, adventitious root (AR) number and length, and primary root (PR) length, compared with the wild-type. Exogenous NAA treatment significantly suppresses PR length in osarf7 mutants, OsARF7-OE lines, and its wild-type, does not affect the root number of osarf7 mutants, but suppresses the biomass of osarf7 mutants. At the molecular level, OsARF7 is preferentially expressed in the culm, root, and leaf, especially highly expressed in the tips of the PR, AR, root pericycle, and lateral root (LR) primordia; meanwhile, OsARF7 expression is significantly enhanced by exogenous NAA treatment, suggesting that the positive regulatory role of OsARF7 on root development is based on auxin signaling. A series of biochemical and genetic analyses demonstrate that OsARF7 functions upstream of OsCRL1 and acts downstream of OsMADS25 to regulate root development via auxin signaling. To conclude, OsARF7 is a key positive regulatory factor that regulates root development by activating the expression of OsCRL1 via auxin signaling, by which, OsMADS25 positively mediates OsARF7 expression in rice. This work provides valuable insight into the regulatory mechanism controlling root development and a genetic resource for the molecular improvement of root architecture.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"115 2","pages":"38"},"PeriodicalIF":3.9,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143516388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0