Plant Molecular Biology最新文献

Author Correction: Glycine rich proline rich protein from Sorghum bicolor serves as an antimicrobial protein implicated in plant defense response. 作者更正：富含甘氨酸的富含脯氨酸的高粱双色蛋白是一种抗菌蛋白，与植物防御反应有关。

IF 3.9 2区生物学

Plant Molecular Biology Pub Date : 2025-04-08 DOI: 10.1007/s11103-025-01585-7

Tanmoy Halder, Gouranga Upadhyaya, Shuddhanjali Roy, Ria Biswas, Arup Das, Angshuman Bagchi, Tanushree Agarwal, Sudipta Ray

引用次数: 0

Cutting-edge computational approaches to plant phenotyping. 植物表型的尖端计算方法。

IF 3.9 2区生物学

Plant Molecular Biology Pub Date : 2025-04-07 DOI: 10.1007/s11103-025-01582-w

Venkatesha Kurumayya

{"title":"Cutting-edge computational approaches to plant phenotyping.","authors":"Venkatesha Kurumayya","doi":"10.1007/s11103-025-01582-w","DOIUrl":"https://doi.org/10.1007/s11103-025-01582-w","url":null,"abstract":"Precision agriculture methods can achieve the highest yield by applying the optimum amount of water, selecting appropriate pesticides, and managing crops in a way that minimises environmental impact. A rapidly emerging advanced research area, computer vision and deep learning, plays a significant role in effective crop management, such as superior genotype selection, plant classification, weed and pest detection, root localization, fruit counting and ripeness detection, and yield prediction. Also, phenotyping of plants involves analysing characteristics of plants such as chlorophyll content, leaf size, growth rate, leaf surface temperature, photosynthesis efficiency, leaf count, emergence time, shoot biomass, and germination time. This article presents an exhaustive study of recent techniques in computer vision and deep learning in plant science, with examples. The study provides the frequently used imaging parameters for plant image analysis with formulae, the most popular deep neural networks for plant classification and detection, object counting, and various applications. Furthermore, we discuss the publicly available plant image datasets for disease detection, weed control, and fruit detection with the evaluation metrics, tools and frameworks, future advancements and challenges in machine learning and deep learning models.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"115 2","pages":"56"},"PeriodicalIF":3.9,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143796149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome-wide exploration of soybean domestication traits: integrating association mapping and SNP × SNP interaction analyses. 大豆驯化性状的全基因组探索：整合关联图谱和SNP × SNP互作分析。

IF 3.9 2区生物学

Plant Molecular Biology Pub Date : 2025-04-03 DOI: 10.1007/s11103-025-01583-9

Abhinandan S Patil, Manoj D Oak, Shreyash Gijare, Aditya Gobade, Santosh Jaybhay, Vilas D Surve, Suresha P G, Dattatraya Salunkhe, Balasaheb N Waghmare, Bhanudas Idhol, Ravindra M Patil, Deepak Pawar

{"title":"Genome-wide exploration of soybean domestication traits: integrating association mapping and SNP × SNP interaction analyses.","authors":"Abhinandan S Patil, Manoj D Oak, Shreyash Gijare, Aditya Gobade, Santosh Jaybhay, Vilas D Surve, Suresha P G, Dattatraya Salunkhe, Balasaheb N Waghmare, Bhanudas Idhol, Ravindra M Patil, Deepak Pawar","doi":"10.1007/s11103-025-01583-9","DOIUrl":"10.1007/s11103-025-01583-9","url":null,"abstract":"Soybean domestication has been essential for crop evolution, adaptation, and modern breeding. Despite advancements in understanding soybean genetics, the genetic basis of DRTs has yet to be fully explored, particularly in the context of genome-wide association studies (GWASs) and gene interaction analyses (epistasis). This study evaluated 198 diverse soybean accessions using 23,574 high-quality SNPs obtained via ddRAD-seq. Nine key DRTs-including those related to seed size (length, width, and thickness), seed coat color, cotyledon color, hypocotyl color, stem growth habit, flower color, pod color, pubescence, and pod-shattering-were phenotyped in two environments. A GWASs conducted via the FarmCPU and BLINK models identified 78 significant SNPs, 14 consistently detected across both environments and models, demonstrating stability. Notably, the SNP rs.Gm16.29778273 linked to pod-shattering resistance. The functional annotation linked three known quantitative trait loci /genes and revealed 11 novel candidate genes associated with DRTs, providing insights into their roles via Gene Ontology (GO) terms. The main effect SNP × SNP interaction analysis revealed that the significant SNP rs.Gm13.16695800 exhibits a pleiotropic effect, controlling both hypocotyl and flower color. Furthermore, 324 epistatic interactions were identified, influencing the expression of DRTs, thereby highlighting the complex genetic architecture underlying these traits. These findings offer valuable insights into domestication and the traits linked to higher yield. They provide a solid foundation for developing marker-assisted selection (MAS) strategies and functional studies to improve soybean breeding for resilient, high-yielding varieties.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"115 2","pages":"55"},"PeriodicalIF":3.9,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143772804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A large-scale gene co-expression network analysis reveals Glutamate Dehydrogenase 2 (GhGDH2_D03) as a hub regulator of salt and salt-alkali tolerance in cotton. 大规模基因共表达网络分析表明，谷氨酸脱氢酶2 （GhGDH2_D03）是棉花耐盐性和盐碱性的枢纽调节因子。

IF 3.9 2区生物学

Plant Molecular Biology Pub Date : 2025-04-02 DOI: 10.1007/s11103-025-01586-6

Rui Hao, Zhan Gao, Xianliang Zhang, Xingxing Wang, Wuwei Ye, Xiugui Chen, Xiongfeng Ma, Xianpeng Xiong, Guanjing Hu

{"title":"A large-scale gene co-expression network analysis reveals Glutamate Dehydrogenase 2 (GhGDH2_D03) as a hub regulator of salt and salt-alkali tolerance in cotton.","authors":"Rui Hao, Zhan Gao, Xianliang Zhang, Xingxing Wang, Wuwei Ye, Xiugui Chen, Xiongfeng Ma, Xianpeng Xiong, Guanjing Hu","doi":"10.1007/s11103-025-01586-6","DOIUrl":"10.1007/s11103-025-01586-6","url":null,"abstract":"Salt stress and salt-alkali stress significantly inhibit the normal growth and development of plants. Understanding the molecular mechanisms of cotton responses to these stresses is crucial for improve yield and fiber quality. In this study, we conducted a comprehensive analysis of the transcriptome dynamics under salt and salt-alkali stress conditions, utilizing 234 RNA-seq datasets compiled from 11 previous studies. After systematic evaluation and correction for batch effects, we observed that root transcriptomes clustered more consistently than leaf transcriptomes across stress treatment and time points. Weighted gene co-expression network analysis (WGCNA) on 123 root transcriptomes identified three key modules, with their hub genes significantly associated with salt and salt-alkali tolerance. Virus-induced gene silencing assay and RNA-seq analysis indicated that GhGDH2_D03 (Gohir.D03G104800), a module hub gene encoding Glutamate Dehydrogenase 2, positively regulates salt and salt-alkali tolerance in cotton by modulating multiple signaling pathways and metabolic processes, including the ethylene signaling pathway. This study underscores the pivotal role of GhGDH2_D03 in conferring tolerance to salt and salt-alkali stress, in addition to its previous reported involvement in biotic stress defense, providing valuable insights and genetic resources for cotton breeding.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"115 2","pages":"54"},"PeriodicalIF":3.9,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143772797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative transcriptomic analysis of heterotic maize development during kernel filling. 杂种玉米灌浆期发育的比较转录组学分析。

IF 3.9 2区生物学

Plant Molecular Biology Pub Date : 2025-04-02 DOI: 10.1007/s11103-025-01584-8

Wenyu Li, Xiangkun Guo, Wen Yao, Keke Li, Qi Zheng, Yongbiao Yu, Zhiwei Zhang, Yan Wang, Weigang Yao, Ju Wu, Huan Hu, Lingwei Hu, Long Zhang, Xinyu Li, Yongbin Dong, Yuling Li

{"title":"Comparative transcriptomic analysis of heterotic maize development during kernel filling.","authors":"Wenyu Li, Xiangkun Guo, Wen Yao, Keke Li, Qi Zheng, Yongbiao Yu, Zhiwei Zhang, Yan Wang, Weigang Yao, Ju Wu, Huan Hu, Lingwei Hu, Long Zhang, Xinyu Li, Yongbin Dong, Yuling Li","doi":"10.1007/s11103-025-01584-8","DOIUrl":"https://doi.org/10.1007/s11103-025-01584-8","url":null,"abstract":"Heterosis, characterized by enhanced performance of a hybrid relative to its parental lines, has been a fundament of plant breeding strategies. Despite the application of heterosis, its molecular mechanisms remain elusive. Here, we focused on the maize heterotic hybrid Yudan132, which showed enhanced agronomic traits compared to its parental lines, including ear and kernel size, kernel weight, and overall yield. Notably, Yudan132 showed increased accumulation of storage substances, characterized by starch, protein contents and grain-filling rates, all of which collectively contribute to the augmented kernel weight. Through gene expression profiling, we identified differentially expressed genes (DEGs) in Yudan132 and its parental lines across four distinct kernel developmental stages (12, 20, 28, and 40 days after pollination). These DEGs displayed both additive and non-additive expression patterns, each contributing to heterosis in maize kernels. The Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis highlighted their involvement in metabolic pathways, biosynthesis of secondary metabolites, carbon metabolism, starch and sucrose metabolism processes. Within these pathways, the enriched DEGs predominantly associated with the gene categories of peroxidase, cytochrome P450, ketoacyl-CoA synthase, and phospholipase D. Furthermore, we identified the transcription factor bZIP88 among the DEGs, which was involved in the regulation of seed size and weight in transgenic Arabidopsis. These results suggested a potential role for bZIP88 in modulating kernel development, thereby further implicating the involvement of the identified DEGs in the molecular mechanisms of heterosis. These findings provide the genetic role of heterosis in kernel and the molecular mechanism regulating kernel development.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"115 2","pages":"53"},"PeriodicalIF":3.9,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143764557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simulation study of factors affecting the accuracy of transcriptome models under complex environments. 复杂环境下影响转录组模型准确性因素的模拟研究。

IF 3.9 2区生物学

Plant Molecular Biology Pub Date : 2025-03-28 DOI: 10.1007/s11103-025-01578-6

Dan Eiju, Yoichi Hashida, Taro Maeda, Koji Iwayama, Atsushi J Nagano

{"title":"Simulation study of factors affecting the accuracy of transcriptome models under complex environments.","authors":"Dan Eiju, Yoichi Hashida, Taro Maeda, Koji Iwayama, Atsushi J Nagano","doi":"10.1007/s11103-025-01578-6","DOIUrl":"https://doi.org/10.1007/s11103-025-01578-6","url":null,"abstract":"Characterization of molecular responses in real and complex field environments is essential for understanding the environmental response of plants. Field transcriptomics prediction consists of modeling of transcriptomes in outdoor fields with various environmental variables: Meteorological parameters, atmospheric gases, soil conditions, herbivores, management, etc. It is the most comprehensive method of studying gene expression dynamics in complex environments. However, it is not clear what factors influence the accuracy of field transcriptome models. In this study, a novel simulation system was developed. Using the system, we performed a large-scale simulation to reveal the factors affecting the accuracy of the models. We found that the factors that had the greatest impact on the accuracy are, in order of importance, the expression pattern of the gene, the number of samples in the training data, the diurnal coverage of the training data, and the temperature coverage of the training data. Validation using actually measured transcriptome data showed similar results to the simulations. Our simulation system and the analysis results will be helpful for developing efficient sampling strategies for training data and for generating simulated data for benchmarking new modelling methods. It will also be valuable to dissect the relative importance of various factors behind transcriptome dynamics in the real environment.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"115 2","pages":"52"},"PeriodicalIF":3.9,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143736203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Physiological and compensatory roles of three starch-branching enzymes in different rice organs. 三种淀粉分支酶在水稻不同器官中的生理和代偿作用。

IF 3.9 2区生物学

Plant Molecular Biology Pub Date : 2025-03-26 DOI: 10.1007/s11103-025-01573-x

Satoko Miura, Naoko Crofts, Nana Koyama, Yuko Hosaka, Ryutaro Morita, Misato Abe, Yasunori Nakamura, Naoko Fujita

{"title":"Physiological and compensatory roles of three starch-branching enzymes in different rice organs.","authors":"Satoko Miura, Naoko Crofts, Nana Koyama, Yuko Hosaka, Ryutaro Morita, Misato Abe, Yasunori Nakamura, Naoko Fujita","doi":"10.1007/s11103-025-01573-x","DOIUrl":"https://doi.org/10.1007/s11103-025-01573-x","url":null,"abstract":"Starch-branching enzymes (BEs) generate amylopectin branches in starch, and three isoforms are found in the rice genome. BEI and BEIIa are expressed ubiquitously, on the other hand BEIIb is exclusively expressed in the endosperm. The loss of BEIIb generates opaque seeds with drastically reduced short amylopectin chains, whereas the loss of BEI slightly reduces long amylopectin chains. However, the loss of BEIIa has no obvious effect on amylopectin structure in the endosperm. Little is known about these isozymes' specific and compensatory roles in other tissues. In this study, all three combinations of double mutants were generated, and their starch properties were analyzed to reveal the function of the solely remaining isozyme, deduce the compensatory function of the missing isozymes, and clarify their roles in different organs. be2a be2b displayed severe sterility and hindered starch synthesis in the endosperm; be1 be2a displayed only minor alterations in endosperm starch; but be1 be2b displayed a drastic increase in amylose content in the endosperm. These results indicate that BEIIa has some compensatory function for BEIIb, and BEI alone is insufficient. Additionally, BEIIa or BEIIb is necessary for endosperm formation. Unlike the endosperm, short amylopectin chains were drastically reduced in the leaf sheath of be1 be2a and be2a be2b, and amylose content was drastically increased in the leaf sheath of be1 be2a and be2a be2b but not be1 be2b. These results indicate that BEIIa has a major role in the leaf sheath, and the generation of short amylopectin chains by BEII can be partially compensated by BEI.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"115 2","pages":"51"},"PeriodicalIF":3.9,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143721003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Elucidating the antioxidant potential of some flavanones as MAO-B inhibitors through DAM, in silico molecular docking and computational analysis. 通过DAM、硅学分子对接和计算分析，阐明一些黄酮类化合物作为MAO-B抑制剂的抗氧化潜力。

IF 3.9 2区生物学

Plant Molecular Biology Pub Date : 2025-03-24 DOI: 10.1007/s11103-025-01567-9

E Athira, S Akhila Darsan, Shinta Davis, Vijisha K Rajan

{"title":"Elucidating the antioxidant potential of some flavanones as MAO-B inhibitors through DAM, in silico molecular docking and computational analysis.","authors":"E Athira, S Akhila Darsan, Shinta Davis, Vijisha K Rajan","doi":"10.1007/s11103-025-01567-9","DOIUrl":"https://doi.org/10.1007/s11103-025-01567-9","url":null,"abstract":"Seven flavanones underwent computational evaluation to determine their effectiveness in filtering UV radiation and scavenging free radicals. The investigated flavanones exhibited enhanced radical scavenging capabilities relative to the parent flavanone, with Hesperidin demonstrating the highest EA and Qmax values, consistent with its antireductant activity. The remaining flavanones displayed lower IE values, suggesting their antioxidant efficacy. Spectroscopic analysis revealed that the HOMO-LUMO and HOMO-1-LUMO transitions are the primary electronic transitions in the UV-Visible spectra of the studied flavanones. Their absorption within the UV-A and UV-B range (260-345 nm) indicates potential utility as UV filters. Theoretical calculations demonstrate that the reactivity of flavanones is concentrated in ring [B], with a reactivity order of 3' > 4' > 2' > 6 > 7 > 5. The BDE values reveal that the 3'-OH group has the lowest value, followed by the 4' position, while hydrogen bonding is responsible for the increased BDE value at position 5. The values of ΔBDE and ΔAIP, relative to phenol, provide a framework for elucidating the preferred mechanism, HAT or SET, underlying the antioxidant behavior. Molecular docking simulations identified hesperetin, 2'-Hydroxyflavanone, 4'-Hydroxyflavanone, Eriodictyol, and Naringenin as potential MAO-B inhibitors, outperforming their synthetic counterparts in this regard.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"115 2","pages":"50"},"PeriodicalIF":3.9,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143701094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of genes associated with soybean protein content using a genome-wide association study. 利用全基因组关联研究检测大豆蛋白含量相关基因。

IF 3.9 2区生物学

Plant Molecular Biology Pub Date : 2025-03-22 DOI: 10.1007/s11103-025-01576-8

Zhiyuan Yu, Bo Hu, Hailong Ning, Wen-Xia Li

{"title":"Detection of genes associated with soybean protein content using a genome-wide association study.","authors":"Zhiyuan Yu, Bo Hu, Hailong Ning, Wen-Xia Li","doi":"10.1007/s11103-025-01576-8","DOIUrl":"https://doi.org/10.1007/s11103-025-01576-8","url":null,"abstract":"The protein content in soybean seeds serves as a crucial measure of soybean quality. Breeding high-protein varieties remains the most cost-effective and efficient approach to increasing soybean protein levels. Nevertheless, limited research has focused on identifying the genes responsible for high protein content among the diverse soybean cultivars. To address this gap, a genome-wide association study (GWAS) was conducted on 455 soybean varieties with varying protein content to predict and validate novel genes involved in regulating protein levels in soybean seeds. Protein content data were obtained from three distinct environments, along with three environmental variables derived from oil content, which is closely related to protein levels. Genotyping was performed using the SoySNP180k BeadChip, yielding genotype data for 63,306 non-redundant single nucleotide polymorphisms (SNPs). Five multi-locus GWAS methods were employed, resulting in the identification of 81 significant quantitative trait nucleotides (QTNs), of which 37 QTNs detected across different methods and environments were further analyzed. Moreover, the simulation platform Blib was used to conduct single-crossing simulation breeding on 81 QTN loci for actual breeding prediction. Haplotype analysis based on re-sequencing data confirmed 2 genes closely linked to protein synthesis, providing a theoretical basis for breeding high-protein soybean varieties and developing molecular breeding strategies.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"115 2","pages":"49"},"PeriodicalIF":3.9,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143692924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Arabidopsis F-box protein FBS associated with the helix-loop-helix transcription factor FAMA involved in stomatal immunity. 拟南芥F-box蛋白FBS与螺旋-环-螺旋转录因子FAMA相关，参与气孔免疫。

IF 3.9 2区生物学

Plant Molecular Biology Pub Date : 2025-03-20 DOI: 10.1007/s11103-025-01577-7

Chunxia Zhang, Junling Yue, Shi Li, Chaoran Zuo, Yi Li, Qixiumei He, Jie Le

{"title":"The Arabidopsis F-box protein FBS associated with the helix-loop-helix transcription factor FAMA involved in stomatal immunity.","authors":"Chunxia Zhang, Junling Yue, Shi Li, Chaoran Zuo, Yi Li, Qixiumei He, Jie Le","doi":"10.1007/s11103-025-01577-7","DOIUrl":"https://doi.org/10.1007/s11103-025-01577-7","url":null,"abstract":"Stomatal pores serve as primary entry points for pathogen invasion. Stomatal closure is a crucial strategy that plants employ to counter pathogen attack. Here, we report that F-BOX STRESS-INDUCED (FBS) is essential for modulating stomatal closure, thereby enhancing resistance to bacteria in Arabidopsis thaliana. The fbs2-1 fbs3-1 fbs4-2 triple mutant displayed increased susceptibility to Pseudomonas syringae pv. tomato (PstDC3000) due to impaired stomatal closure. Additionally, FBS4 interacts with and degrades the basic helix-loop-helix (bHLH) transcription factor FAMA. Both the fama-1 single mutant plants and the fama-1 fbs2-1 fbs3-1 fbs4-2 quadruple mutant plants exhibited resistance to PstDC3000 inoculation. Furthermore, the expression levels of abscisic acid (ABA)-responsive genes RD29A, RD29B, ABI2, and CIPK25 were altered in the fbs2-1 fbs3-1 fbs4-2 and fama-1 mutant plants. Collectively, our data demonstrate that FBS, in association with FAMA, plays an important role in pathogen invasion by influencing ABA signaling-related stomatal closure.","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"115 2","pages":"48"},"PeriodicalIF":3.9,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143670659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0