Plant Molecular Biology最新文献

{"title":"Elucidating putative novel functions of the AINTEGUMENTA-LIKE 7 transcription factor in clubroot resistance in Arabidopsis.","authors":"Kethmi Nirmani Jayawardhane, Tharangani K Somarathna, Victor P Manoli, Sheau-Fang Hwang, Stephen E Strelkov, Stacy D Singer, Guanqun Chen","doi":"10.1007/s11103-026-01698-7","DOIUrl":"10.1007/s11103-026-01698-7","url":null,"abstract":"<p><p>Clubroot caused by Plasmodiophora brassicae is a devastating soilborne disease of the Brassicaceae worldwide. Disease management often relies on disease resistant cultivars, but their overuse has led to the break down of resistance. As such, it is important to elucidate novel gene targets for clubroot tolerance. AINTEGUMENTA-LIKE 7 (AIL7), a transcription factor in the AP2/ERF family, has been observed to influence the expression of genes involved in biotic stress response when overexpressed in Arabidopsis seeds in our previous study. Here we evaluated the resistance of Arabidopsis lines that constitutively overexpressed AIL7, as well as an ail7 T-DNA mutant, to P. brassicae, and found that both mutant and overexpression lines displayed reduced clubroot symptoms and pathogen spore load in infected tissues. Targeted gene expression analysis revealed significant alterations in the transcript levels of genes linked to pathogen response, along with notable differences in those associated with salicylic acid (SA) and jasmonic acid (JA) defense pathways. Interestingly, the SA- and JA-related genes had a tendency for up-regulation in AIL7 overexpression and mutant lines in the absence, rather than presence, of P. brassicae, and phytohormone analyses confirmed these results. These findings indicate that phytohormone-mediated defense pathways are constitutively activated in Arabidopsis lines in which AIL7 is either overexpressed or knocked out, which likely contributes to a reduction in disease symptoms. Taken together, these findings suggest that AIL7 could be a potential target for improving plant responses to clubroot. However, more extensive evaluation across a broader range of inoculum levels and pathotypes will be necessary to assess its relevance for breeding resistant cultivars.</p>","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"116 2","pages":""},"PeriodicalIF":3.8,"publicationDate":"2026-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147514267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of the methionine sulfoxide reductase (MSR) gene family in four Triticum species and functional analysis of TaMSRB5 involved in copper stress tolerance in Arabidopsis thaliana.","authors":"Heng Chen, Luzhen Wang, Kexin Zhao, Shuai Yan, Yifei Chen, Lei Guo, Yinying Wu, Renmei Tian, Danping Li, Wenjia Zhang, Jianjun Liu, Haosheng Li, Xiaoyan Duan, Xiukun Liu, Yulong Song, Donghong Min, Xinyou Cao, Xin Gao","doi":"10.1007/s11103-026-01703-z","DOIUrl":"10.1007/s11103-026-01703-z","url":null,"abstract":"<p><p>Methine sulfoxide reductase (MSR) plays a crucial role in protecting plants from oxidative damages. However, their involvement in copper (Cu) detoxification remains largely uncharacterized. In this study, we identified 17 MSR genes in wheat (Triticum aestivum), 12 MSR genes in Triticum dicoccoides (Td), 6 in Triticum urartu (Tu), and 5 in Aegilops tauschii (Aet). Strong collinearity and conservation were found among orthologs. Three constitutively expressed homoeologs of TaMSRB5 were significantly upregulated in leaves and downregulated in roots of wheat seedlings when exposed to excessive Cu. Overexpression of TaMSRB5 in Arabidopsis significantly enhanced Cu tolerance as evidenced by increased fresh weight and root length in transgenic plants, compared to wild-type (WT, Col-0). Under Cu toxicity, Arabidopsis overexpression lines accumulated similar levels of Cu in their leaves and roots compared with WT. However, they exhibited elevated levels of oxidized glutathione (GSSG), total glutathione (T-GSH), along with Glutathione S-transferase (GST), superoxide dismutase (SOD) and catalase (CAT) activities. Simultaneously, they showed reduced levels of reactive oxygen species (ROS) and malondialdehyde (MDA), and a lower GSH/ GSSG ratio, indicating enhanced ROS scavenging ability. Consistent with the moderation of oxidative stress, comparative transcriptome analysis revealed that four GST genes were upregulated in transgenic Arabidopsis plants under Cu stress, suggesting the potential relationship between these genes with TaMSRB5 in Cu detoxification. The findings demonstrate the pivotal role of TaMSRB5 in enhancing Cu tolerance in Arabidopsis and provide novel insights into the molecular mechanisms underlying Cu detoxification.</p>","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"116 2","pages":""},"PeriodicalIF":3.8,"publicationDate":"2026-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147514328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The prospects of targeting messenger RNA surveillance factor PELOTA for developing virus resistance in plants.","authors":"Anik Majumdar, Yeluru Mohan Babu, Firoz Mondal, Mehulee Sarkar, Prantik Mazumder, Priyanka Pradhan, Ravinder Kumar, Sota Koeda, Anirban Roy","doi":"10.1007/s11103-026-01700-2","DOIUrl":"10.1007/s11103-026-01700-2","url":null,"abstract":"","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"116 2","pages":""},"PeriodicalIF":3.8,"publicationDate":"2026-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12999637/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147481416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Changes in Alcohol dehydrogenase gene expression throughout strawberry fruit ripening process.","authors":"Daniel Bustos, David Alarcón-Muñoz, Francisca Arriaza-Rodríguez, Felipe Moraga, Gabriela Urra, Carolina Parra-Palma, Francisca Hormazabal-Abarza, Patricio Ramos, Luis Morales-Quintana","doi":"10.1007/s11103-026-01691-0","DOIUrl":"10.1007/s11103-026-01691-0","url":null,"abstract":"","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"116 2","pages":""},"PeriodicalIF":3.8,"publicationDate":"2026-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147481467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"γ-Valerolactone promotes Solanum lycopersicum seedling growth through transcriptomic and plant hormone changes.","authors":"Fangfang Ren, Hui Shen, Weiye Chen, Siyue Sha, Shanshan Li, Xin Tao, Zhuorui Li, Weishou Shen, Nan Gao","doi":"10.1007/s11103-026-01699-6","DOIUrl":"10.1007/s11103-026-01699-6","url":null,"abstract":"","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"116 2","pages":""},"PeriodicalIF":3.8,"publicationDate":"2026-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147459348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"OsFPN1 functions as an essential metal transporter for Fe homeostasis and Co/Ni tolerance in rice.","authors":"Jindong Wu, Hiromi Nakanishi","doi":"10.1007/s11103-026-01701-1","DOIUrl":"10.1007/s11103-026-01701-1","url":null,"abstract":"","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"116 2","pages":""},"PeriodicalIF":3.8,"publicationDate":"2026-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12987785/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147459294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome wide analysis of RLK-Pelle-L-LEC family in Rosa chinensis and negative regulation of black spot disease by RcPLLEC45.","authors":"Xingyu Liu, Xiaowen Zheng, Haijiao Wang, Tingliang Xu","doi":"10.1007/s11103-026-01694-x","DOIUrl":"10.1007/s11103-026-01694-x","url":null,"abstract":"","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"116 2","pages":""},"PeriodicalIF":3.8,"publicationDate":"2026-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147444565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid selection of early-maturing and low-ear position maize hybrids suitable for China using genomic prediction.","authors":"Ping Luo, Wenzhe Li, Lin Zhang, Jie Yang, Houwen Wang, Fei Wang, Ruisi Yang, Xinlong Xu, Ke Yang, Hongjun Yong, Mingshun Li, Jianfeng Weng, Degui Zhang, Zhiqiang Zhou, Jienan Han, Wenwei Gao, Junjie Fu, Xinhai Li, Zhiyong Ni, Zhuanfang Hao","doi":"10.1007/s11103-026-01693-y","DOIUrl":"10.1007/s11103-026-01693-y","url":null,"abstract":"","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"116 2","pages":""},"PeriodicalIF":3.8,"publicationDate":"2026-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147355971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differential activity of CYTOKININ OXIDASE/DEHYDROGENASE3 (CKX3) and CKX5 genes in regulating yield components in Brassica napus L.","authors":"Ireen Schwarz, Christian Möllers, Thomas Schmülling","doi":"10.1007/s11103-025-01678-3","DOIUrl":"10.1007/s11103-025-01678-3","url":null,"abstract":"<p><p>Cytokinin is a plant hormone that regulates several yield-related traits in plants. Previously, it was demonstrated that in tetraploid oilseed rape (Brassica napus L.), mutation of all four cytokinin-degrading BnCKX3 and both BnCKX5 genes resulted in increased cytokinin concentration, larger and more active inflorescence meristems, and a higher number of ovules per gynoecium. This resulted in the formation of more flowers and pods on the main stem, thereby increasing seed yield from the main stem of the plants. Here, we investigated the relative contributions of distinct combinations of BnCKX3 and BnCKX5 genes of the A and C genomes to these yield components. Our analysis revealed an unexpectedly strong role for BnCKX5 in regulating these traits and identified distinct supportive BnCKX3 gene mutant combinations. These findings facilitate the selection of relevant alleles for breeding. Furthermore, seeds from BnCKX gene mutant plants showed oil content and concentrations of unsaturated fatty acids similar to those of the wild type. Taken together, this study provides further insight into the role of cytokinin and BnCKX genes in regulating yield components in oilseed rape and provides novel information on functionally relevant alleles.</p>","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"116 2","pages":""},"PeriodicalIF":3.8,"publicationDate":"2026-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12963132/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147355939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide association mapping, phylogenetic study, and multivariate analysis of component traits of grain yield in wheat under heat stress conditions.","authors":"Ezhumalai Sivapragasam, Sukumar Taria, Sudhir Kumar, Hari Krishna, Narayana Bhat Devate, Biswabiplab Singh, Elangovan Allimuthu, Dhandapani Raju, Ranjit Ranjan Kumar, Rabi Narayan Sahoo, Monika Dalal, Renu Pandey, Viswanathan Chinnusamy","doi":"10.1007/s11103-026-01695-w","DOIUrl":"10.1007/s11103-026-01695-w","url":null,"abstract":"<p><p>Heat stress negatively impacts key yield-contributing physiological traits in wheat, leading to a decrease in grain yield. Scanning of genomic regions linked to these traits, along with the identification of the most relevant candidate genes (CGs), is an effective strategy for developing heat-tolerant wheat cultivars in the near future. In this context, a genome-wide association mapping approach has been employed to identify chromosomal regions associated with these traits, along with to identify the putative CGs for heat tolerance in wheat. Genotyping was performed using the 35 K Axiom Wheat Breeder Array. From our study, principal component analysis (PCA) revealed that biomass (BM), canopy temperature (CT), and seed weight per pot (SWPP) explained a higher cumulative variance. Population structure and diversity analysis filtered 13,947 markers and revealed three subpopulations with sufficient diversity. A large whole-genome LD block size of 7.15 MB was obtained at a half LD decay value. We have mapped 14 significant MTAs linked to these traits with - log10(p) value > 5.44 after Bonferroni correction and also identified 14 high-confidence CGs. Our study also identified four haplotype groups, suggesting the potential for a haplotype-based breeding program under heat stress. Promoter analysis revealed 174 cis-regulatory elements (CREs). Phylogenetic analysis of the pleiotropic gene TraesCS7A02G200200 revealed three major clades of closely related species. We have also reported several orthologous genes related to our 14 major CGs. Untranslated regions (UTRs) analysis found several upstream Open Reading Frames (uORFs) in few identified genes, which can be employed to understand the stringent mechanism of gene regulation under heat stress. By using the Multitrait-genotype ideotype index (MGIDI), we have selected 13 high-performance genotypes for their use as donor parent for heat tolerance. Henceforth, after successful validation, these SNPs can be utilized for marker-assisted transfer of genes/QTLs to develop heat-tolerant wheat cultivars.</p>","PeriodicalId":20064,"journal":{"name":"Plant Molecular Biology","volume":"116 2","pages":""},"PeriodicalIF":3.8,"publicationDate":"2026-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147355967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}