Non-tissue culture genetic modifications for plant improvement.

IF 3.9 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Molecular Biology Pub Date : 2025-05-16 DOI:10.1007/s11103-025-01594-6

Xin-Xin Li, Yue-Hao Gao, Hao-Wen Ma, Yu-Qiong Wang, Tian Bu, Weilun Yin, Xinli Xia, Hou-Ling Wang

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引用次数: 0

Abstract

Gene delivery systems are essential for investigating gene regulation mechanisms and enhancing the genetic improvement of functional traits in plants. However, fewer than 0.1% of higher plant species on Earth can be genetically modified. Even for these species, the genetic modification process relies on complex tissue culture methods, which are time-consuming, costly, and often require specialized technical skills. Additionally, the efficiency of genetic modification is extremely low in some species. Notably, over the past five years, significant progress has been made in establishing non-tissue culture genetic modification systems. This advancement effectively resolved a series of previously mentioned challenges and innovated in biotechnology for the improvement of many valuable plant species. This review summarizes the research advancements in non-tissue culture genetic modification technologies and presents examples of successful species modified using various methods, including fast-treated Agrobacterium co-culture (Fast-TrACC), cut-dip-budding (CDB), particle bombardment, and nano-mediated delivery systems. Additionally, we propose a working guideline to classify, analyze, evaluate, and select non-tissue culture genetic modification systems for plant species of interest. Our review also discusses the potential for enhancing plant regeneration capacity, improving genetic modification efficiency, and the future application prospects for plant improvement.

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植物改良的非组织培养遗传修饰。

基因传递系统是研究植物基因调控机制和促进植物功能性状遗传改良的重要手段。然而，地球上只有不到0.1%的高等植物物种可以进行基因改造。即使对这些物种来说，基因改造过程也依赖于复杂的组织培养方法，这既耗时又昂贵，而且往往需要专门的技术技能。此外，某些物种的基因改造效率极低。值得注意的是，近五年来，在建立非组织培养转基因系统方面取得了重大进展。这一进展有效地解决了前面提到的一系列挑战，并为许多有价值的植物物种的改良提供了生物技术方面的创新。本文综述了非组织培养转基因技术的研究进展，并介绍了采用快速处理农杆菌共培养（Fast-TrACC）、切浸出芽（CDB）、粒子轰击和纳米介导传递系统等多种方法对植物进行转基因的成功案例。此外，我们还提出了一个分类、分析、评估和选择感兴趣的植物物种的非组织培养转基因系统的工作指南。本文还讨论了转基因技术在提高植物再生能力、提高转基因效率方面的潜力，以及未来在植物改良方面的应用前景。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plant Molecular Biology 生物-生化与分子生物学

自引率

2.00%

发文量

审稿时长

1.4 months

期刊介绍： Plant Molecular Biology is an international journal dedicated to rapid publication of original research articles in all areas of plant biology.The Editorial Board welcomes full-length manuscripts that address important biological problems of broad interest, including research in comparative genomics, functional genomics, proteomics, bioinformatics, computational biology, biochemical and regulatory networks, and biotechnology. Because space in the journal is limited, however, preference is given to publication of results that provide significant new insights into biological problems and that advance the understanding of structure, function, mechanisms, or regulation. Authors must ensure that results are of high quality and that manuscripts are written for a broad plant science audience.