How it all begins: molecular players of the early graviresponse in the non-elongating part of flax stem.

Abstract

Plants have developed two major strategies to adjust their position in response to gravity: differential cell growth on opposing sides of elongating regions and complex processes in non-elongating stem parts, such as the development of reaction wood. Gravistimulation of flax plants induces gravitropic curvature in non-elongating stem parts, largely associated with modifications in phloem and xylem fibers. To gain insight into the key "triggers" and "forward players" that induce negative gravitropic reactions, transcriptome profiling of phloem fibers and xylem tissues from the pulling and opposite stem sides was conducted 1 and 8 h after gravistimulation. The first observed reaction was the activation of processes associated with RNA synthesis and protein folding in both tissues and stem sides, followed by the activation of kinases and transferases. Transcriptomic data revealed rapid and substantial shifts in chloroplast metabolism across all analyzed tissues, including the temporal activation of the branched-chain amino acid pathway, adjustments to light-harvesting complexes, and jasmonic acid biosynthesis. Notably, auxin transporter genes were activated only in the xylem, while other auxin-related genes showed minimal upregulation 1 h after stem inclination in any analyzed sample. Asymmetric changes between stem sides included the sharp activation of ethylene-related genes in the phloem fibers of the opposite stem side, as well as tertiary cell wall deposition in both the phloem and xylem fibers of the pulling stem side during the later stages of the graviresponse. These results provide valuable insights into the mechanisms underlying plant response to gravity.