Parasite最新文献

{"title":"Phenology and parasitism rates of Xenos oxyodontes and Xenos moutoni (Strepsiptera: Xenidae) in Vespa (Hymenoptera: Vespidae) in South Korea.","authors":"Jaehee Kim, Chang-Jun Kim, Moon Bo Choi, Ohseok Kwon","doi":"10.1051/parasite/2026028","DOIUrl":"10.1051/parasite/2026028","url":null,"abstract":"<p><p>The long-term seasonal parasitism patterns, hereafter referred to as stylopization, and within-nest developmental stage distributions of Vespa-associated Xenidae (Strepsiptera) remain poorly characterized in South Korea. We compiled 15 years of data (2008-2023) based on trapped Vespa individuals from multiple regions and quantified stylopization rates for two Xenidae species, Xenos oxyodontes and Xenos moutoni, based on diagnostic characters visible on stylopized hosts. Seasonal occurrence was summarized primarily from female Xenos specimens, which can be identified to species, and host-association patterns were assessed where possible. To complement the trap-based records, we dissected 22 Vespa analis colonies collected in September 2020 and documented strepsipteran developmental stages within the nests. Across 39,610 examined host individuals, stylopization rates and seasonal occurrence differed between the two Xenos species and among host taxa, indicating distinct host-use and temporal activity patterns. Importantly, the nest dissections provided direct observations of late-season developmental stage compositions, enabling us to compare within-nest reproductive/developmental stages to those inferred from trap-detected stylopized hosts. This comparison revealed that key reproductive-stage information may not be captured by trap records alone. Thus, integrating long-term trap data with nest-level observations can clarify species-specific phenology and developmental/reproductive stage distributions in Vespa-associated Xenidae, providing a better empirical context for interpreting stylopization intensity and developmental timing in field populations.</p>","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"33 ","pages":"28"},"PeriodicalIF":2.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13186533/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147974399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel 3R-compliant ex vivo approach to assess ectoparasiticide repellency against Phlebotomus perniciosus in dogs.","authors":"Ambre Sibari, Guillem Weis-Servat, Emmanuel Liénard, Ali Salem, Thomas Blondel, Marie Varloud, Emilie Bouhsira","doi":"10.1051/parasite/2026029","DOIUrl":"10.1051/parasite/2026029","url":null,"abstract":"<p><p>Ectoparasiticides are essential for preventing insect bites and transmission of arthropod-borne pathogens such as Leishmania spp. Traditionally, their anti-feeding efficacy is evaluated in vivo using sedated dogs exposed to sand flies. To comply with 3R principles (replacement, reduction and refinement) and to avoid animal exposure, this study aimed to develop an ex vivo feeding model to assess the efficacy of a dinotefuran, permethrin, and pyriproxyfen (DPP) [Vectra^®3D] combination against Phlebotomus perniciosus as an alternative to in vivo testing. Twelve dogs were assigned to either a DPP-treated group (n = 6) or an untreated control group (n = 6). On days -7, 1, 7, 14, 21, and 28, dogs were sedated and exposed for one hour to 50 (±5) female sand flies. In parallel, hair collected from each dog was used in an ex vivo artificial feeding system. After exposure, sand flies were assessed for feeding and survival rates. Anti-feeding efficacy was the primary criterion, while insecticidal efficacy was evaluated as a secondary outcome. In both models, control groups showed feeding and survival rates above 31% and 90%, confirming a reliable challenge. The treated group showed significantly lower feeding and survival rates than the control group (p < 0.05). In both models, DPP demonstrated a strong anti-feeding effect in dogs for one month, with efficacy above 80%. This first direct comparison of in vivo and ex vivo models confirms DPP's fast and lasting protection against Ph. perniciosus and positions the ex vivo model as a promising 3R-aligned alternative for studying vector-borne disease prevention.</p>","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"33 ","pages":"33"},"PeriodicalIF":2.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13233030/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148157499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular surveillance of intestinal parasites and Acanthamoeba species in soils from outdoor built environments in rural northwestern Argentina.","authors":"María Cristina Almazán, Melisa Evangelina Díaz-Fernández, Elvia Ester Nieves, Marisa Juárez, Lucia Estela Mejia, Emilio Rey Mejia, Alejandro Javier Krolewiecki, Philip J Cooper, Rubén Oscar Cimino, Rojelio Mejia","doi":"10.1051/parasite/2026039","DOIUrl":"10.1051/parasite/2026039","url":null,"abstract":"<p><strong>Background: </strong>Soil-transmitted helminths (STHs) and other enteric parasites remain endemic in rural communities of northwestern Argentina. Environmental surveillance may provide additional information on transmission dynamics and community exposure.</p><p><strong>Results: </strong>We analyzed 116 soil samples collected from 28 sites, including 27 households in Solazuti and one rural school in El Cedral (Orán Department, Salta Province, Argentina), using multi-parallel real-time quantitative PCR targeting intestinal protozoa, STHs, zoonotic nematodes, and Acanthamoeba spp. Parasites were detected in outdoor built environments, with 44% of Solazuti households showing at least one positive sample. Ascaris lumbricoides and Toxocara canis were the most frequently detected helminths and showed distinct spatial patterns. Detection of A. lumbricoides was strongly associated with the presence of other parasites (OR 18.11, 95% CI 8.30-38.6; p < 0.0001). Additionally, A. lumbricoides-positive households harbored significantly more parasite species than A. lumbricoides-negative households (p = 0.0343), and A. lumbricoides frequency correlated with parasite richness (Spearman r = 0.4145, p = 0.0316). Acanthamoeba spp. was nearly ubiquitous (96.4% of sites), including the school environment, with a 75% contamination rate.</p><p><strong>Conclusions: </strong>Molecular analysis of soil samples revealed spatially heterogeneous but sustained exposure to multiple pathogens in rural outdoor environments. Soil-based qPCR may complement traditional surveillance by providing community-level data independent of human sampling.</p>","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"33 ","pages":"41"},"PeriodicalIF":2.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13427259/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148649264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A fast and reliable molecular method to detect Anisakidae in zooplanktonic Euphausiacea.","authors":"Anaïs Delhomme, Mélanie Gay, Alice Delegrange, Odile Bourgau, Maureen Duflot, Pierre Cresson","doi":"10.1051/parasite/2026038","DOIUrl":"10.1051/parasite/2026038","url":null,"abstract":"<p><p>Anisakidae is a family of marine nematodes, largely monitored in fish and cephalopods due to their zoonotic risk for human consumers. However, there is a need to further study the ecology of this family to better predict infection dynamics, particularly as global change affects marine ecosystem functioning generally, and specifically Anisakidae behaviour and infection levels, in line with the principles of the One Health approach. However, in-depth studies are hampered by technical difficulties in collecting data on members of the cycle that are not consumed by humans, namely zooplankton. This results in a glaring imbalance in terms of available data between fish and cephalopods on the one hand, and zooplankton on the other. Development of robust, rapid, and efficient methods for the detection of Anisakidae in zooplankton is therefore needed. Euphausiacea is considered to be the major zooplanktonic group hosting Anisakidae larvae. We therefore calibrated a PCR technique followed by Sanger sequencing to determine the presence of Anisakidae larvae in zooplanktonic Euphausiacea from the North Sea. This calibration was based in particular on the creation of artificially infested zooplanktonic samples, on which several molecular methods have been tested. When calibrated, we then applied this method to a small set of zooplanktonic samples and performed a preliminary parasitological survey. Prevalence values were higher (2.5% to 4.5%) than those reported in the literature, either due to the increased sensitivity of the molecular method and/or to changes in ecosystem functioning. This method has clear potential to push forward an integrated understanding of Anisakidae infection in zooplankton.</p>","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"33 ","pages":"39"},"PeriodicalIF":2.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13412147/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148606125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic characterisation of Cryptosporidium spp., Encephalitozoon spp., and Enterocytozoon bieneusi in European brown hare (Lepus europaeus) in Central Europe.","authors":"Alena Konvalinová, Marie Křesťanová, Nikola Holubová, Jana Fenclová, Marta Kicia, Matúš Rajský, Pavel Beran, Bohumil Sak, Martin Kváč","doi":"10.1051/parasite/2026042","DOIUrl":"10.1051/parasite/2026042","url":null,"abstract":"<p><p>The European brown hare (Lepus europaeus) is an ecologically important small game mammal whose populations have declined in many European countries. A total of 370 faecal samples collected between 2022 and 2024 from hares in the Czechia and Slovakia were molecularly screened for the presence of Cryptosporidium spp. (18S rRNA, gp60), Giardia intestinalis (TPI), Encephalitozoon spp., and Enterocytozoon bieneusi (ITS). qPCR analysis confirmed the presence of Cryptosporidium spp. (n = 15; 4.1%; CI: 2.5-6.6%), Encephalitozoon spp. (n = 34; 9.2%; CI: 6.7-12.6%), and E. bieneusi (n = 16; 4.3%; CI: 2.6-6.9%). Sequence analyses revealed the presence of C. parvum (n = 2), C. sciurinum (n = 3), C. ubiquitum (n = 5), and Cryptosporidium sp. deer mouse genotype IV (n = 5). At the gp60 locus, C. parvum belonged to subtypes IIaA15G1R1 and IIaA23G1R1, C. sciurinum to subtypes VIIIcA10G2R1 (n = 2) and VIIIbA11G1R1 (n = 1), and C. ubiquitum to subtypes XIIa (n = 3) and XIId (n = 2). Five E. bieneusi genotypes were identified: D (n = 8), CHALT1 (n = 1), WildBoar3 (n = 1), CHN-F1 (n = 2), and C (n = 4). Encephalitozoon sequencing revealed E. cuniculi genotypes I (n = 6), II (n = 26), and III (n = 1), and E. hellem (n = 1). Giardia intestinalis was not detected. Cryptosporidium sp. deer mouse genotype IV is reported here for the first time in Europe.</p>","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"33 ","pages":"43"},"PeriodicalIF":2.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13446113/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148679434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bothriocestus claviceps (Cestoda: Bothriocephalidae) in pike in southwestern France (Teleostei: Esocidae): molecular evidence.","authors":"Laura Jamet, Vincent Haÿ, Mélyne Hautecœur, Marion Escarpit, Vincent Renard, Clarice Salaun, Louis Guyon, Agathe Lindemann, Aude Jabbari, Aurélia L'Hostis, Gaël P J Denys, Fabienne Audebert","doi":"10.1051/parasite/2026046","DOIUrl":"10.1051/parasite/2026046","url":null,"abstract":"<p><p>Bothriocestus claviceps (Goeze, 1782) is a cestode parasite infecting several fish species. One of its principal definitive hosts is the European eel Anguilla anguilla. The parasite is widely distributed across Europe and has been reported from numerous countries, such as Poland, Czechia, and France. In southern France, the European eel co-occurs with the Aquitanian pike, Esox aquitanicus, an endemic species. This pike is threatened by human activities and hybridization with the introduced Northern pike Esox lucius and is therefore classified as \"vulnerable\" on the French Red List of the International Union for Conservation of Nature (IUCN). Despite its conservation status, little information is available regarding its biology, particularly its parasitofauna, which remains undocumented. Over a three-year period, Aquitanian pike were collected and examined for parasites. Cestodes recovered from the intestinal tract were analyzed using a molecular approach. Molecular identification of the species was based on partial sequences of 28S ribosomal DNA and cytochrome oxidase subunit I (COI). Morphometric data were also obtained to provide additional data on the identified cestode species. Our results provide the first evidence of the presence of B. claviceps in the intestine of E. aquitanicus, as well as in one hybrid (E. aquitanicus × E. lucius), and suggest that these fish can act as definitive or post-cyclic hosts for this cestode species. This study contributes new insights into the parasitofauna of the Aquitanian pike and expands current knowledge of B. claviceps.</p>","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"33 ","pages":"46"},"PeriodicalIF":2.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13521508/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148831429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Another host, another mystery: Infection of polychaete Pista palmata (Annelida: Terebellidae) with a new blood fluke (Digenea: Aporocotylidae) in the coastal Northwest Atlantic Ocean.","authors":"Anna N Chowansky, Kristina M Hill-Spanik, Dennis E Kyle, Isaure de Buron","doi":"10.1051/parasite/2026048","DOIUrl":"10.1051/parasite/2026048","url":null,"abstract":"<p><p>Terebelliform annelids can serve as intermediate hosts for blood flukes of marine fishes and sea turtles. The scarcity of global reports on the life cycles of these parasites reflects the need for further exploration. Our objective was to further our knowledge of the diversity and life cycles of blood flukes in coastal South Carolina (SC), United States. Four species of terebellids (Enoplobranchus sanguineus, Amphitrite ornata, Pista palmata, and Streblosoma hartmanae) were collected (total N = 201) at low tide from September 2025 to February 2026 at two localities in SC. Twelve specimens of three terebellid species were infected, and parasites were identified by sequencing the LSU and ITS2 regions of ribosomal RNA and the COI mitochondrial DNA gene. Enoplobranchus sanguineus (12.9% prevalence, 4/31) and A. ornata (3.6% prevalence, 2/55) were infected with the previously reported blood flukes Neospirorchis sp. Neogen14 (Unicaecidae) and Cardicola parvus (Aporocotylidae), respectively. No specimens of S. hartmanae were infected (0/86). One new aporocotylid with mature sporocysts containing microcercous cercariae was discovered in P. palmata (20.7% prevalence, 6/29). Phylogenetic analysis revealed that sequences of the new aporocotylid were in a well-supported clade with sequences of Braya spp. and Cardicola abu. The fish host of this parasite has yet to be discovered to fully elucidate the life cycle of this new aporocotylid.</p>","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"33 ","pages":"49"},"PeriodicalIF":2.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13552996/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148912985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative assessment of immunochromatographic test kits using low-molecular-weight antigens from cyst fluids of two different genotypes of Taenia solium for serodiagnosis of human cysticercosis.","authors":"Lakkhana Sadaow, Penchom Janwan, Patcharaporn Boonroumkaew, Rutchanee Rodpai, Oranuch Sanpool, Tongjit Thanchomnang, Marcello Otake Sato, Pewpan M Intapan, Hiroshi Yamasaki, Yasuhito Sako, Toni Wandra, Kadek Swastika, Wanchai Maleewong","doi":"10.1051/parasite/2026003","DOIUrl":"10.1051/parasite/2026003","url":null,"abstract":"<p><p>Human cysticercosis is a serious zoonosis caused by infection with larvae (cysticerci) of the pork tapeworm, Taenia solium. Infection can involve the nervous system, causing chronic headache and intracranial hypertension, focal neurological deficits, epileptic seizures, and paralysis. The disease is found in developing countries, where porcine cysticercosis is prevalent and undercooked pork is habitually consumed. This study aimed to develop immunochromatography-based test (ICT) kits, using low-molecular-weight antigens purified from cyst fluids of Latin American and Asian genotypes of T. solium. To evaluate the kits, we used 164 serum samples, including 24 from proven/confirmed cysticercosis cases, 110 from cases with other parasitoses, and 30 from healthy individuals. Diagnostic performances were calculated. The sensitivity, specificity, and accuracy were 83.3% (95% CI [62.6-95.3]), 93.6% (95% CI [88.1-97.0]), and 92.1% (95% CI [86.8-95.7]), respectively for the American genotype-based ICT kit, while for the Asian genotype-based ICT kit, they were 87.5% (95% CI [67.6-97.3]), 98.6% (95% CI [94.9-99.8]), and 97.0% (95% CI [93.0-99.0]), respectively. The sensitivity and specificity did not significantly differ between the two ICT kits (exact McNemar's test; p > 0.05), with a concordance of 93.9%, represented by a Cohen's kappa of 0.77 (p < 0.001), indicating substantial agreement. These results indicate that affinity-purified antigens from different geographical isolates can be used for the diagnosis of human cysticercosis. The diagnostic specificities were better than for a previously reported ICT kit that used crude antigen.</p>","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"33 ","pages":"3"},"PeriodicalIF":2.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12829318/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146030610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phenotypic changes in natural populations of Anopheles gambiae s.l. at the onset of the long dry season in tropical savannahs of Burkina Faso, West Africa.","authors":"Wadaka Mamai, Karine Mouline, David Renault, Kevin Hidalgo, Kounbobr Roch Dabiré, Frédéric Simard","doi":"10.1051/parasite/2026010","DOIUrl":"10.1051/parasite/2026010","url":null,"abstract":"<p><p>In the tropical savannahs with long dry seasons, malaria mosquito populations virtually disappear after the drying up of breeding sites to reappear in large numbers at the onset of next rainy season. While aestivation and long-distance migration are proposed as key strategies enabling these vectors to persist through the dry-season, the physiological, biochemical, and morphological traits underpinning these mechanisms remain insufficiently explored, particularly under natural field conditions. This study explored seasonal changes in Anopheles coluzzii, An. gambiae, and An. arabiensis at the onset of the dry season in the harsh savannahs of Burkina Faso, West Africa. Late-instar immature specimens were collected from two ecologically distinct sites, one with permanent and the other with only temporary breeding habitats, during the rainy season and the transitional period into the dry season. Larvae were reared to adulthood under natural conditions and several traits were analysed including ovarian development, sub-cuticular fat body hypertrophy, body size, and energy reserves. Gonotrophic dissociation was significantly more frequent in An. coluzzii at the onset of the dry season, indicating a shift toward reproductive arrest. All three species exhibited increased body size and cuticular fat deposits during the transitional period, though with species-specific differences. Notably, only An. coluzzii showed significant increases in energy reserves (proteins, lipids, and carbohydrates) during the transition period. These adaptive responses differed between the study sites, suggesting the influence of breeding habitats. The findings highlight that species within the An. gambiae complex engage in distinct phenotypic trajectories at the onset of the dry season, suggesting divergent adaptations and trade-offs in energy acquisition and allocation to survive during the dry season.</p>","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"33 ","pages":"9"},"PeriodicalIF":2.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12934212/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147284719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of OvaCyte for the detection of gastrointestinal parasites in ovine and bovine animals: comparison with traditional flotation techniques.","authors":"Nagwa Elghryani, Geetika Lahan, Jayanta Bor Gohain, Eileen Collins, Trish McOwan, Theo de Waal","doi":"10.1051/parasite/2026002","DOIUrl":"10.1051/parasite/2026002","url":null,"abstract":"<p><p>This study aimed to evaluate the diagnostic and analytical performance of OvaCyte, an automated image-based system for the detection of gastrointestinal parasites in ruminants, against traditional flotation techniques. OvaCyte is available in two versions: OvaCyte Plus, which automates egg detection and quantification, and OvaCyte Premium, which incorporates enhanced analysis to differentiate parasite families, genera, and species within strongyles (e.g., Trichostrongylidae, Haemonchus contortus, and Nematodirus spp.). Coccidia are also classified as standard or type B, with the latter specifically including Eimeria weybridgensis, Eimeria crandallis and Eimeria ovinoidalis, based on distinct morphometrical features. The identification of Haemonchus contortus was validated using peanut agglutinin (PNA) fluorescence staining as the gold standard. Sensitivity and specificity for OvaCyte Plus, Mini-FLOTAC, and McMaster were calculated based on a consensus \"true status\". Limits of detection and quantification were calculated using regression analysis. OvaCyte Plus demonstrated the highest sensitivity, especially for Nematodirus (95%), coccidia (93%), strongyles (92%), and Strongyloides papillosus (90%). Mini-FLOTAC showed moderate sensitivity (63-79%), while McMaster had the lowest value (30-76%). For Moniezia spp., sensitivity was similar for OvaCyte Plus and Mini-FLOTAC (79%), but lower for McMaster (59%). Specificity was high across all techniques (92-100%). Differences in performance were attributed to varying multiplication factors: OvaCyte Plus (3 EPG), Mini-FLOTAC (7.5 EPG), and McMaster (50 EPG). Strong correlations were observed between OvaCyte Plus and manual techniques for strongyle egg counts. OvaCyte Premium exhibited the highest sensitivity across all parasites. OvaCyte Plus and Premium demonstrated performance comparable to or exceeding traditional techniques for the detection of gastrointestinal parasites in ruminants.</p>","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"33 ","pages":"8"},"PeriodicalIF":2.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12934211/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147284727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}