Parasite最新文献_第2页

Molecular analysis of DHFR and DHPS gene mutations in Plasmodium cynomolgi from humans and macaques in Southeast Asia. 东南亚人类和猕猴食蟹疟原虫DHFR和DHPS基因突变的分子分析。

IF 2.4 2区医学

Parasite Pub Date : 2025-01-01 Epub Date: 2025-09-29 DOI: 10.1051/parasite/2025057

Raweewan Sangsri, Nathjanan Jongkon, Kiattawee Choowongkomon, Suchinda Malaivijitnond, Nicholas P J Day, Arjen M Dondorp, Mallika Imwong

{"title":"Molecular analysis of DHFR and DHPS gene mutations in Plasmodium cynomolgi from humans and macaques in Southeast Asia.","authors":"Raweewan Sangsri, Nathjanan Jongkon, Kiattawee Choowongkomon, Suchinda Malaivijitnond, Nicholas P J Day, Arjen M Dondorp, Mallika Imwong","doi":"10.1051/parasite/2025057","DOIUrl":"10.1051/parasite/2025057","url":null,"abstract":"Plasmodium cynomolgi is an emerging zoonotic malaria parasite in Southeast Asia, infecting both humans and macaques. In this study, we investigated mutations in the DHFR and DHPS genes of P. cynomolgi from humans and macaques, comparing them to known resistance mutations in P. falciparum and P. vivax. We also examined how these mutations affect antifolate drug binding, which may influence treatment efficacy and resistance. Nine asymptomatic human blood samples from Cambodia and 29 macaque samples from Thailand were analyzed. Human samples included eight P. cynomolgi monoinfections and one mixed infection with P. vivax, while all macaque samples were monoinfections. The PcyDHFR and PcyDHPS genes were amplified, sequenced, and subjected to haplotype analysis. Human samples from Battambang, Cambodia were 100% identical to the P. cynomolgi RO strain, showing no DHFR mutations and one DHPS mutation (V451I). In contrast, macaque samples from Saraburi, Thailand showed PcyDHFR mutations N44T and C49S, and two haplotypes based on I7 variation - haplotype 1 (72.41%) with wild-type I7 and haplotype 2 (27.59%) with the I7 mutation. PcyDHPS mutations were identical across macaque isolates. Protein structures of PcyDHFR and PcyDHPS were modeled using SWISS-MODEL, focusing on the N- and C-terminals. Mutations occurred near catalytic sites but did not significantly affect binding affinity, based on molecular docking with eight antifolate drugs. These findings suggest that current antifolate drugs remain potentially effective against P. cynomolgi, and highlight the importance of monitoring drug resistance in zoonotic malaria.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"32 ","pages":"64"},"PeriodicalIF":2.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12479033/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145192319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Screening for the ciliate Buxtonella sulcata in free-ranging dairy cattle on Terceira Island, Azores archipelago. 亚速尔群岛特塞拉岛散养奶牛中纤毛虫短孔虫的筛查。

IF 2.3 2区医学

Parasite Pub Date : 2025-01-01 Epub Date: 2025-03-28 DOI: 10.1051/parasite/2025014

Mário Ribeiro, Sara Gomes-Gonçalves, Alexandra Silva, Guilherme Moreira, Eric Viscogliosi, Magali Chabé, João Rodrigo Mesquita

{"title":"Screening for the ciliate Buxtonella sulcata in free-ranging dairy cattle on Terceira Island, Azores archipelago.","authors":"Mário Ribeiro, Sara Gomes-Gonçalves, Alexandra Silva, Guilherme Moreira, Eric Viscogliosi, Magali Chabé, João Rodrigo Mesquita","doi":"10.1051/parasite/2025014","DOIUrl":"10.1051/parasite/2025014","url":null,"abstract":"Buxtonella sulcata is an alveolate ciliate protist, historically considered a commensal of the gastrointestinal tract of cattle. Despite the fact that its cysts are morphologically identical to those of Balantioides coli, molecular identification techniques have shed new light on its role as a pathogen. This work aimed to assess the presence of this ciliate in the population of dairy cattle on the Azorean island of Terceira by means of molecular analyses (ITS1-5.8S-ITS2 rRNA) of stool samples. A total of 116 samples were collected from adult Holstein-Friesian dairy cows, with no signs of gastrointestinal disease. A proportion of 49.1% of the samples were PCR-positive for Bu. sulcata, and 12 different genetic sequences were identified. These findings highlight the need for future research concerning the factors that influence the presence of Bu. sulcata in the gastrointestinal tract of dairy cows, the role of bovines as possible sources of infection, and the impact this ciliate may have on the health, welfare, and productivity of these animals.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"32 ","pages":"21"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11951862/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143731172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spatial distribution of Ixodes ricinus in forest habitats: a comparative study of the northern and southern slopes of Mount Slavnik, Slovenia. 森林生境中蓖麻伊蚊的空间分布：斯洛文尼亚斯拉夫尼克山南北坡的比较研究。

IF 2.4 2区医学

Parasite Pub Date : 2025-01-01 Epub Date: 2025-07-25 DOI: 10.1051/parasite/2025044

Jure Jugovic, Alenka Babič, Anka Kuhelj, Vladimir Ivović

{"title":"Spatial distribution of Ixodes ricinus in forest habitats: a comparative study of the northern and southern slopes of Mount Slavnik, Slovenia.","authors":"Jure Jugovic, Alenka Babič, Anka Kuhelj, Vladimir Ivović","doi":"10.1051/parasite/2025044","DOIUrl":"10.1051/parasite/2025044","url":null,"abstract":"While previous studies have examined the elevational limits of Ixodes ricinus in Central Europe, this study is the first to investigate the influence of slope orientation on tick distribution in Slovenia. Our results provide new insights into how two important microclimatic factors, temperature and humidity, vary between the northern and southern slopes, and how these differences affect the abundance of I. ricinus, a factor that has not been studied in this region before. We found that nymph density was highest on the southern slope at intermediate elevations (720 m) and increased with temperature. In contrast, fewer adult ticks were found on the southern slope compared to the northern slope, most of them at 720 m elevations. The significantly higher abundance of adult ticks on the northern slope is probably related to the cooler temperatures, higher relative humidity, denser ground vegetation and greater availability of hosts such as roe deer. Although these results are regionally specific, they contribute to a more comprehensive understanding of the ecological factors influencing the distribution of I. ricinus in Central Europe.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"32 ","pages":"46"},"PeriodicalIF":2.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12291548/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144708335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prevalence and subtype distribution of Blastocystis infections among community participants in Thailand: a systematic review and meta-analysis. 泰国社区参与者囊虫感染的患病率和亚型分布：一项系统综述和荟萃分析。

IF 2.4 2区医学

Parasite Pub Date : 2025-01-01 Epub Date: 2025-08-19 DOI: 10.1051/parasite/2025042

Manas Kotepui, Supaluk Popruk, Kwuntida Uthaisar Kotepui, Frederick Ramirez Masangkay, Kinley Wangdi, Aongart Mahittikorn, Christen Rune Stensvold

{"title":"Prevalence and subtype distribution of Blastocystis infections among community participants in Thailand: a systematic review and meta-analysis.","authors":"Manas Kotepui, Supaluk Popruk, Kwuntida Uthaisar Kotepui, Frederick Ramirez Masangkay, Kinley Wangdi, Aongart Mahittikorn, Christen Rune Stensvold","doi":"10.1051/parasite/2025042","DOIUrl":"10.1051/parasite/2025042","url":null,"abstract":"A comprehensive understanding of the prevalence and subtype distribution of Blastocystis infections among community participants in Thailand is essential to inform targeted public health interventions. This systematic review and meta-analysis aimed to estimate the overall prevalence of Blastocystis infections and to determine the distribution of subtypes among community participants in Thailand. Relevant studies on Blastocystis infections in community participants in Thailand were searched in PubMed, Embase, Scopus, Ovid, ProQuest, and the Thai-Journal Citation Index. The methodological quality of the included studies was assessed using the Joanna Briggs Institute critical appraisal tools. Prevalence estimates and subtype distributions were calculated using random-effects models. A total of 947 articles were identified, with 60 studies included in the systematic review and meta-analysis. The meta-analysis led to an estimated overall prevalence of Blastocystis infections in community participants in Thailand at 8.34% (95% CI: 5.48%-12.51%; I2: 98.2%; number of studies: 60; number of participants: 33,101). Meta-regression analysis showed no significant temporal trends in infection prevalence. The highest prevalence rates were observed in Eastern Thailand (13.54%) and Western Thailand (10.09%). Subtype analysis identified ST3 and ST1 as the most common subtypes, accounting for 50.05% and 23.50% of positive samples, respectively. The highest prevalence was reported in military personnel (29.87%), followed by orphans (29.01%). Improved use of molecular and culture-based diagnostic methods is recommended to enhance detection accuracy. Public health interventions should prioritize high-risk groups, such as military personnel and orphans, and address regional disparities to reduce the burden of Blastocystis infections.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"32 ","pages":"53"},"PeriodicalIF":2.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12364436/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144883410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Repositioning of moxidectin: a promising approach in cutaneous leishmaniasis therapy. 莫西丁的重新定位：皮肤利什曼病治疗的一个有前途的方法。

IF 2.3 2区医学

Parasite Pub Date : 2025-01-01 Epub Date: 2025-07-04 DOI: 10.1051/parasite/2025035

Lynn Al Samra, Mohamad El Nahas, Ilham Mneimneh, Sima Tokajian, Georges Nemer, Aia Sinno, Kelven Rahy, Sergio Thoumi, Zahraa Zibara, Ahmad El Khatib, Dalal Sabbagh, Jacques Guillot, Louna Karam, Lazo Ali, Ruqaya Baghdadi, Charbel Al Khoury

{"title":"Repositioning of moxidectin: a promising approach in cutaneous leishmaniasis therapy.","authors":"Lynn Al Samra, Mohamad El Nahas, Ilham Mneimneh, Sima Tokajian, Georges Nemer, Aia Sinno, Kelven Rahy, Sergio Thoumi, Zahraa Zibara, Ahmad El Khatib, Dalal Sabbagh, Jacques Guillot, Louna Karam, Lazo Ali, Ruqaya Baghdadi, Charbel Al Khoury","doi":"10.1051/parasite/2025035","DOIUrl":"10.1051/parasite/2025035","url":null,"abstract":"Cutaneous leishmaniasis presents a significant challenge to public health due to its diverse clinical manifestations, resistance development, and treatment-related adverse effects. Here, we examined the efficacy of ivermectin, moxidectin (MOX), afoxolaner, and permethrin against all stages of Leishmania tropica and THP-1 cells. We also assessed the potential for resistance acquisition after 15 rounds of artificial selection. To elucidate the mode of action of MOX, we employed RNA sequencing, molecular dynamics simulation, and chloride flux assays. Additionally, we evaluated the therapeutic index of MOX using the Galleria mellonella infection model. MOX demonstrated the highest selectivity index against leishmaniasis (promastigotes: 0.58 μM; amastigotes: 0.96 μM; host cells: 60.29 μM). Moreover, MOX exhibited the lowest resistance acquisition in both promastigotes and intracellular amastigotes after 15 rounds of artificial selection, with resistance ratios of 17.23 and 4.59, respectively. Post-exposure to MOX, differential gene expression profiles showed both stage-specific and stage-unspecific enrichment of gene families involved in crucial biological processes. Moreover, molecular dynamics simulations revealed a potential neutralizing effect of MOX on the chloride channel of L. tropica. Specifically, MOX binds to the selectivity filter, potentially disrupting the osmotic equilibrium and thereby killing the parasite. The in vivo introduction of MOX significantly inhibited the growth of L. tropica in G. mellonella larvae, resulting in decreased rates of mortality and melanization. These findings indicate that MOX is a promising candidate for the treatment of L. tropica infections, warranting further investigation and potential consideration for clinical use.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"32 ","pages":"42"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12232414/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144560737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expansion of the known host range of Giardia duodenalis and Cryptosporidium spp. in captive wildlife at Beijing Zoo. 北京动物园圈养野生动物十二指肠贾第鞭毛虫和隐孢子虫已知宿主范围的扩大。

IF 2.3 2区医学

Parasite Pub Date : 2025-01-01 Epub Date: 2025-01-23 DOI: 10.1051/parasite/2024079

Qianming Zhao, Zhiyang Pei, Yongqiang He, Ting Jia, Yanzhen Zhang, Mengjun Zheng, Zhenjie Zhang, Meng Qi

{"title":"Expansion of the known host range of Giardia duodenalis and Cryptosporidium spp. in captive wildlife at Beijing Zoo.","authors":"Qianming Zhao, Zhiyang Pei, Yongqiang He, Ting Jia, Yanzhen Zhang, Mengjun Zheng, Zhenjie Zhang, Meng Qi","doi":"10.1051/parasite/2024079","DOIUrl":"10.1051/parasite/2024079","url":null,"abstract":"Giardia duodenalis and Cryptosporidium spp. are significant zoonotic parasites that cause diarrhea and affect a diverse range of hosts. This study aimed to investigate the infection rates of these parasites in captive wildlife at Beijing Zoo. A total of 445 fecal samples were collected and analyzed using PCR. The infection rate of G. duodenalis was 3.82% (17/445), with assemblage A (n = 1), assemblage B (n = 13), and assemblage E (n = 3) identified. The infection rate of Cryptosporidium spp. was 0.22% (1/445), with only one instance of C. ryanae identified from cattle (Bos taurus). To the best of our knowledge, this study enhances the understanding of the host range of G. duodenalis in captive wildlife within China, highlighting infections in Corsac Fox (Vulpes corsac), Indian Rhinoceros (Rhinoceros unicornis), and Giraffe (Giraffa camelopardalis). These findings indicate that the infection rates of G. duodenalis and Cryptosporidium spp. in captive wildlife at Beijing Zoo are low, while showing that the known host range of G. duodenalis is expanding.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"32 ","pages":"4"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11756238/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143024300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prevalence and genetic diversity of Pentatrichomonas hominis in pig populations in Guangdong and Anhui Provinces, China. 广东和安徽猪群人五毛单胞菌流行率及遗传多样性

IF 2.3 2区医学

Parasite Pub Date : 2025-01-01 Epub Date: 2025-06-11 DOI: 10.1051/parasite/2025027

Pengyun Lu, Yibin Zhu, Haiming Cai, Hanqin Shen, Siyun Fang, Dingai Wang, Zhuanqiang Yan, Shenquan Liao, Nanshan Qi, Minna Lv, Xuhui Lin, Yongle Song, Xiangjie Chen, Jianfei Zhang, Juan Li, Mingfei Sun

{"title":"Prevalence and genetic diversity of Pentatrichomonas hominis in pig populations in Guangdong and Anhui Provinces, China.","authors":"Pengyun Lu, Yibin Zhu, Haiming Cai, Hanqin Shen, Siyun Fang, Dingai Wang, Zhuanqiang Yan, Shenquan Liao, Nanshan Qi, Minna Lv, Xuhui Lin, Yongle Song, Xiangjie Chen, Jianfei Zhang, Juan Li, Mingfei Sun","doi":"10.1051/parasite/2025027","DOIUrl":"10.1051/parasite/2025027","url":null,"abstract":"Pentatrichomonas hominis is a protozoan parasite that infects the gastrointestinal tract of humans and mammals, causing abdominal pain and diarrhea. However, its presence in pigs and its potential as a pathogen causing diarrhea in piglets have not been well studied. This study aimed to investigate the prevalence of P. hominis in pigs and its potential for zoonotic transmission. A total of 406 pig fecal samples were collected from four pig farms located in Guangdong and Anhui Provinces. Fecal DNA extraction was carried out using a commercially available kit. A nested PCR methodology was employed to detect the presence of P. hominis infection. Samples that tested positive were subsequently subjected to sequencing, and the genetic characteristics of the 18S ribosomal RNA (rRNA) gene were analyzed. The overall infection rate of P. hominis was 34.98% (142/406), in Guangdong Province 28.47% (80/281), and in Anhui Province 49.60% (62/125). Among different age groups, suckling piglets had the highest infection rate at 40.24% (68/169). Genetic analysis of the P. hominis isolates showed that the PH-1 genotype was predominant and had a high degree of similarity to P. hominis sequences obtained from humans, cats, and pigs, indicating the potential for zoonotic transmission. The high infection rate and genetic diversity highlight the need for effective control measures in pig farming to reduce parasite transmission and zoonotic risk.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"32 ","pages":"33"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12158214/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pathology of fatal Baylisascaris schroederi infection in a wild giant panda. 野生大熊猫施氏贝利蛔虫致死性感染的病理分析。

IF 2.3 2区医学

Parasite Pub Date : 2025-01-01 Epub Date: 2025-06-11 DOI: 10.1051/parasite/2025026

Lingling Chang, Danhui Zhang, Yashi Wang, Zun Ren, Yaping Wu, Qiang Zhang, Guanghui Zhao, Guanglin Pan, Xinglong Wang, Xiaomin Zhao, Dewen Tong

{"title":"Pathology of fatal Baylisascaris schroederi infection in a wild giant panda.","authors":"Lingling Chang, Danhui Zhang, Yashi Wang, Zun Ren, Yaping Wu, Qiang Zhang, Guanghui Zhao, Guanglin Pan, Xinglong Wang, Xiaomin Zhao, Dewen Tong","doi":"10.1051/parasite/2025026","DOIUrl":"10.1051/parasite/2025026","url":null,"abstract":"Baylisascaris schroederi McIntosh, 1939 (Ascarididae), a nematode specific to giant pandas (Ailuropoda melanoleuca), is a major health threat, particularly to wild populations. A 20-year-old wild adult female giant panda rescued from a Chinese nature reserve died with a 2-month history of emaciation and weakness. Necropsy was performed. Grossly, the giant panda was very thin with minimal fat stores throughout, and marked serous atrophy of fat around the kidneys. Mesenteric edema was very pronounced in the posterior intestine. The abdominal cavity contained approximately 5 L of orange-yellow, translucent, low-viscosity fluid. There were ca. 1,660 robust ascarids occupying the lumen of the esophagus, stomach, and intestines. Microscopically, the intestine showed moderate necrotizing and eosinophilic enteritis with adult nematodes, consistent with an ascarid. PCR and sequencing confirmed that the ascarid species was B. schroederi. This case highlights a fatal B. schroederi infection in a wild giant panda, with malnutrition and possible multiple organ failure identified as the primary causes of death.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"32 ","pages":"34"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12158236/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prevalence and characterization of Cryptosporidium in sika deer from Northern China. 中国北方梅花鹿隐孢子虫的流行及特征。

IF 2.3 2区医学

Parasite Pub Date : 2025-01-01 Epub Date: 2025-06-11 DOI: 10.1051/parasite/2025029

Yan Tang, Nian-Yu Xue, Yang Gao, Zhen-Qiu Gao, Hong-Di Zhuang, Guang-Rong Bao, Hong-Wei Cao, Jing Liu, Jian-Ming Li, Shuo Liu

{"title":"Prevalence and characterization of Cryptosporidium in sika deer from Northern China.","authors":"Yan Tang, Nian-Yu Xue, Yang Gao, Zhen-Qiu Gao, Hong-Di Zhuang, Guang-Rong Bao, Hong-Wei Cao, Jing Liu, Jian-Ming Li, Shuo Liu","doi":"10.1051/parasite/2025029","DOIUrl":"10.1051/parasite/2025029","url":null,"abstract":"Cryptosporidium spp. are important zoonotic parasites that can cause moderate to severe diarrhea in humans and animals. However, the epidemiological data of Cryptosporidium in sika deer in China need to be updated. In this study, a total of 466 fecal samples were collected from sika deer in Shandong, Jilin, Liaoning, and Heilongjiang provinces. Nested PCR was used to amplify the SSU rRNA gene to detect Cryptosporidium spp. The results showed that the overall infection rate of Cryptosporidium spp. was 14.81%, with no significant differences among regions (p = 0.05). The highest infection rate was found in Heilongjiang Province (23.60%) and the lowest in Jilin Province (10.71%). The infection rate in summer (23.61%) seemed higher than that in autumn (13.20%), but the difference was not statistically significant (p = 0.30). Notably, young sika deer showed a significantly higher infection rate (28.21%) compared to adults (10.32%) (p < 0.0001). Sequence analysis identified two Cryptosporidium species/genotypes: Cryptosporidium deer genotype (98.55%) and Cryptosporidium ubiquitum (1.45%). Subtyping revealed that the C. ubiquitum isolate belonged to the zoonotic XIIa subtype. These findings provide new insights into the prevalence and genetic diversity of Cryptosporidium in sika deer and suggest that sika deer may act as a potential reservoir for zoonotic Cryptosporidium transmission.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"32 ","pages":"35"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12158220/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioassay tests reveal for the first time pyrethroid resistance in Aedes mosquitoes from Franceville, southeast Gabon, Central Africa. 生物测定试验首次显示中非加蓬东南部Franceville的伊蚊对拟除虫菊酯类杀虫剂产生耐药性。

IF 2.3 2区医学

Parasite Pub Date : 2025-01-01 Epub Date: 2025-07-01 DOI: 10.1051/parasite/2025036

Judicaël Obame-Nkoghe, Faël Moudoumi Kondji, El Hadji Diouf, Omar Thiaw, Brad Ghaven Niangui, Arnauld Ondo-Oyono, Yasmine Okomo-Nguema, Neil Michel Longo-Pendy, Franck Mounioko, Boris Makanga, Basile Kamgang, Christophe Paupy, Pierre Kengne, Patricks Voua Otomo, El Hadji Amadou Niang

{"title":"Bioassay tests reveal for the first time pyrethroid resistance in Aedes mosquitoes from Franceville, southeast Gabon, Central Africa.","authors":"Judicaël Obame-Nkoghe, Faël Moudoumi Kondji, El Hadji Diouf, Omar Thiaw, Brad Ghaven Niangui, Arnauld Ondo-Oyono, Yasmine Okomo-Nguema, Neil Michel Longo-Pendy, Franck Mounioko, Boris Makanga, Basile Kamgang, Christophe Paupy, Pierre Kengne, Patricks Voua Otomo, El Hadji Amadou Niang","doi":"10.1051/parasite/2025036","DOIUrl":"10.1051/parasite/2025036","url":null,"abstract":"The spread of resistance to insecticides, such as pyrethroids, in Aedes vectors increases the risk of spread of arboviral diseases. In Gabon, the insecticide resistance profiles of Ae. aegypti and Ae. albopictus species remain poorly known. During a study to monitor the dynamics of Aedes populations in Franceville, in south-east Gabon, the resistance profiles of these two species to pyrethroids, organophosphates and carbamates were assessed. Susceptibility tests on adults and synergist tests with piperonyl butoxide (PBO) were carried out as per the World Health Organization protocol. The results showed that Ae. aegypti and Ae. albopictus were susceptible to permethrin, pirimiphos-methyl and bendiocarb. However, both species were resistant to deltamethrin (mortality: 67% for Ae. aegypti; 33% for Ae. albopictus). Exposure to a 5-fold dose of deltamethrin increased mortality to 100% and 91% for Ae. aegypti and Ae. albopictus, respectively. Resistance to alpha-cypermethrin was also recorded (mortality: 82% for Ae. aegypti; 64.6% for Ae. albopictus). Pre-exposure to PBO resulted in the restoration of susceptibility to deltamethrin and alpha-cypermethrin for Ae. aegypti, and a significant increase in mortality for Ae. albopictus. These data provide the first evidence of pyrethroid resistance in Aedes in Gabon and could help to establish more effective control measures against arbovirus vectors.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"32 ","pages":"40"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12212768/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144541807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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