Parasite最新文献

{"title":"Stomoxys calcitrans as a potential mechanical vector of Anaplasma phagocytophilum: assessment through original ex vivo feeding models.","authors":"Clotilde Rouxel, Guillem Weis-Servat, Emmanuel Liénard, Shukri Sharif, Anne-Claire Lagrée, Pierre Lucien Deshuillers, Henri-Jean Boulouis, Nadia Haddad, Emilie Bouhsira","doi":"10.1051/parasite/2026021","DOIUrl":"https://doi.org/10.1051/parasite/2026021","url":null,"abstract":"<p><p>Granulocytic anaplasmosis is a zoonotic disease that affects various domestic mammals (dogs, horses, and, more rarely, cats). In ruminants, it is better known as tick-borne fever (TBF) and is responsible for significant economic losses on European livestock farms, mainly due to a drop in milk production, abortions, and immunosuppression, which can lead to secondary infections. The disease is caused by the strictly intracellular bacterium Anaplasma phagocytophilum, whose biological vectors are ticks of the genus Ixodes. Other blood-feeding arthropods may be involved in transmitting this bacterium, notably Stomoxys calcitrans, a major ectoparasite of livestock that is implicated in transmitting other pathogens, including bacteria of the genus Anaplasma. This study aimed to evaluate the potential of S. calcitrans to act as a mechanical vector of A. phagocytophilum under laboratory conditions. Two experimental models were employed: one mimicking immediate transmission, and the other delayed transmission. In both models, A. phagocytophilum DNA and RNA were detected in S. calcitrans for the first time, but no traces of the bacterium's DNA or RNA were found in the glass feeder's blood. Further research is needed to confirm these findings through field studies investigating the presence of the bacterium in flies under natural conditions. This study also describes two original infection models of stable flies designed to reproduce their ex vivo blood-feeding, promoting alternative experimental approaches in accordance with animal welfare regulations and 4R principles.</p>","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"33 ","pages":"25"},"PeriodicalIF":2.4,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13105450/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147777961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Module-level recombination drives DBLMSP polymorphism and functional conservation in Plasmodium falciparum.","authors":"Yi-Wen Duan, Shen-Bo Chen, Tian-Yu Wang, Wan-Xuan Yang, Kokouvi Kassegne, Hai-Mo Shen, Jun-Hu Chen","doi":"10.1051/parasite/2026024","DOIUrl":"10.1051/parasite/2026024","url":null,"abstract":"<p><p>The DBLMSP gene family in Plasmodium falciparum encodes surface antigens involved in immune evasion and red blood cell invasion, yet its extensive polymorphism has long defied straightforward classification. While analyzing DBLMSP1 sequences from samples collected along the China-Myanmar border, we found that haplotypes could not be readily explained by standard population genetic models. Instead, comparative alignment and BLAST analysis revealed that DBLMSP1 and DBLMSP2 consist of discrete, recombinable sequence modules, flanked by conserved upstream and downstream regions. This led us to propose a modular framework that redefines allele structure as combinations of well-defined building blocks with consistent boundaries and positional constraints. Through global mining of DBLMSP sequences, we identified nine genotypes each for DBLMSP1 and DBLMSP2, with modules labeled sequentially (e.g., 1M2a, 1M3c, and 2M3b). Some modules were shared across paralogs, notably the identical sequence of DBLMSP1 module 1M3c and DBLMSP2 module 2M3a, suggesting historical inter-locus recombination. In the dominant genotype DBLMSP1-1, nucleotide diversity and Tajima's D peaked within variable modules, whereas conserved structural elements, including the receptor-binding cleft and SPAM domain, were under purifying selection. Patterns of long-range linkage disequilibrium aligned with module junctions, suggesting that modular structure may shape recombination patterns independently of selection. Modular recombination has been widely recognized in viral systems and multigene families such as var, but its relevance in DBLMSPs has been underappreciated. By applying this framework to P. falciparum DBLMSPs, we aim to provide a useful perspective for understanding their structural diversity and evolutionary dynamics, with implications for immunogen design and parasite surveillance.</p>","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"33 ","pages":"24"},"PeriodicalIF":2.4,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13088805/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147699446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Glossina from the Republic of the Congo: species identification by MALDI-TOF MS and research of associated micro-organisms.","authors":"Irina Babakana Bemba, Zaina Amirat, Philippe Parola, Christophe Antonio Nkondjio, Arsene Lenga, Lionel Almeras, Adama Zan Diarra","doi":"10.1051/parasite/2026007","DOIUrl":"10.1051/parasite/2026007","url":null,"abstract":"<p><p>Human African trypanosomiasis (HAT) and Animal African trypanosomiasis (AAT), transmitted by Glossina species, remain major health and economic burdens in Africa. Accurate vector identification is essential for effective control strategies. However, current identification methods of Glossina species based on morphological and/or molecular techniques have several limitations that often hinder reliable species-level classification. This study assessed matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) as an alternative or complementary approach to morphological and molecular methods for Glossina species identification and explored its ability to detect infection status. A total of 265 tsetse flies were collected and morphologically classified into the Glossina palpalis group (n = 200) and the Glossina fuscipes group (n = 65), later confirmed by molecular analysis as Glossina palpalis palpalis and Glossina fuscipes quanzensis, respectively. Spectra were generated from wings, legs, and thoraxes to identify the most suitable body parts. For G. p. palpalis, high-quality spectra were obtained from wings (98.0%), legs (96.5%), and thoraxes (93.5%); for G. f. quanzensis, corresponding values were 89.2%, 87.7%, and 72.3%. Blind testing showed that 89.5% of spectra for G. p. palpalis and 95.2% for G. f. quanzensis matched morphological identification, with 87.0% and 94.6%, respectively, reaching relevant score thresholds. Molecular screening detected Trypanosoma congolense DNA in nine specimens, but MALDI-TOF MS spectra could not distinguish infected from uninfected flies. These findings demonstrate that MALDI-TOF MS is a rapid, reliable tool for Glossina species identification, particularly using wings and legs, but is unsuitable for infection status determination.</p>","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"33 ","pages":"5"},"PeriodicalIF":2.4,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12875062/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146126038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fasciolosis, a foodborne zoonotic trematode infection in cattle in Bangladesh: multifaceted validation of parthenogenecity and anthelmintic efficacy.","authors":"Haydar Ali, Shahadat Hossain, Sharmin Shahid Labony, Aminul Islam, Mohammad Mehedi Hasan, Anita Rani Dey, Mahmudul Alam, Abu Hadi Noor Ali Khan, Abdul Alim, Anisuzzaman","doi":"10.1051/parasite/2026004","DOIUrl":"10.1051/parasite/2026004","url":null,"abstract":"<p><p>Parthenogenic Fasciola (Trematoda: Fasciolidae) flukes have been developed by the hybridization of Fasciola hepatica and Fasciola gigantica. They are aspermic (asF) but capable of clonal expansion through parthenogenesis and are spreading rapidly throughout the globe. Here, we unambiguously prove the occurrence of parthenogenic Fasciola in cattle in Bangladesh, along with their ex vivo culture protocol and anthelmintic efficacy. By employing multiple conventional and molecular tools, we confirmed the presence of both the spermic F. gigantica (sFg) (31.7%; 814/2575) and asF (68.3%; 1761/2575) in Bangladesh. Both the adult sFg and asF survived well in DMEM supplemented with 20% bovine serum and 20% bovine bile. Using a DMEM-based ex vivo culture protocol, we found that nitroxynil (NTX) and oxyclozanide (OCZ) efficiently killed both sFg and asFg in a concentration and time-dependent manner. Surprisingly, triclabendazole (TCBZ) and clorsulon (CRL) killed neither sFg nor asF. Also, praziquantel, albendazole, and levamisole did not affect the viability of the flukes. We found that all TCBZ survivors had more than one mutation, both in nucleotides (G440A, G643A, and G788A) and amino acids (R147K, E215K, and R263K) of the binding pocket of carboxylesterase B (CestB), providing molecular evidence of TCBZ resistance in Fasciola. Taken together, asF constitutes more than two-thirds of the Fasciola population in Bangladesh. This study unambiguously proved the ineffectiveness of TCBZ against both asF and sFg circulating in Bangladesh. Therefore, only OCZ and NTX remain effective against fasciolosis, which thus poses ongoing public health risks of infection in humans with TCBZ-tolerant strains of fasciolosis.</p>","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"33 ","pages":"7"},"PeriodicalIF":2.4,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12892867/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146158049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel RFLP-PCR method for the rapid diagnosis of Echinococcus multilocularis and different Echinococcus granulosus sensu lato species from tissue samples.","authors":"Gérald Umhang, Jean-Marc Boucher, Lisa Laboutière, Grégory Karadjian","doi":"10.1051/parasite/2026017","DOIUrl":"10.1051/parasite/2026017","url":null,"abstract":"<p><p>Both alveolar echinococcosis and cystic echinococcosis are zoonotic diseases affecting thousands of humans in Europe annually. Five Echinococcus species are known to be endemic in Europe, but with varying relative importance in humans and animals. In order to identify these Echinococcus species from tissue samples, an RFLP-PCR method was developed and validated in accordance with the French quality standards for molecular diagnostics (NF U47-600-2). Amplification by PCR of about 846 bp from the 12S ribosomal RNA gene was followed by a single digestion step with three restriction enzymes (AccI, DdeI and HinfI). The RFLP-PCR method distinguishes Echinococcus multilocularis, E. granulosus sensu stricto, E. equinus, E. ortleppi and E. canadensis in six specific profiles composed of two or three bands. Each profile is specific for the species, except an additional specific profile from genotype G8 of E. canadensis. Species from other genera of the Taeniidae family correspond to two other types of profiles. Additionally, in silico analyses predicted three specific additional profiles for the other four Echinococcus species. The limit of detection of the method including DNA extraction is estimated to be a minimum of 1,000 copies. Total specificity and sensitivity were obtained by testing a panel of 73 and 79 DNA samples previously identified by sequencing. This RFLP-PCR technique is cost-effective, simple and rapid to implement, making it suitable for use in large epidemiological studies, as well as for single diagnostic purposes targeting tissue samples, such as cysts or worms, without requiring sequencing.</p>","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"33 ","pages":"17"},"PeriodicalIF":2.4,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13041236/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147593418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First analysis of Spirometra mansoni excretory-secretory proteins by the 4D-DIA method.","authors":"Fei Gao, Xuan Xuan Song, Jie Hao, Cheng Yue Cao, Shuang Li, Xi Zhang","doi":"10.1051/parasite/2026025","DOIUrl":"https://doi.org/10.1051/parasite/2026025","url":null,"abstract":"<p><p>In this study, adults and plerocercoids of S. mansoni were cultivated in vitro to systematically analyze the components of the excretory-secretory proteins (ESPs) of Spirometra mansoni. Afterwards, the differentially expressed proteins (DEPs) were identified and protein components were examined using the Data Independent Acquisition (DIA) mode. A total of 944 proteins were identified, including 580 plerocercoid-specific proteins, whereas no specific proteins were found in adults. Quantitative analysis revealed that 607 proteins were significantly differentially expressed, with 390 upregulated in the plerocercoid group, and 217 upregulated in the adult group. Gene Ontology functional annotation revealed that the upregulated proteins in the plerocercoid group were significantly enriched in functions such as nitrogen compound metabolism, proteasome core complexes, and ion binding. Kyoto Encyclopedia of Genes and Genomes pathway enrichment revealed that the DEPs were strongly correlated with signal transduction, signal transportation, and catabolism pathways. Moreover, metabolic network analysis revealed that key pathways included the pentose phosphate pathway and glycolysis/gluconeogenesis. In addition, indirect ELISA revealed that immunization of mice with ESPs induced a Th1/Th2 mixed immune response, dominated by a Th1 response. Cytokine detection further verified that ESPs had good immunogenicity, and could activate both humoral and cellular immune responses. This study revealed, for the first time, the differential expression profile of ESPs between adults and plerocercoids of S. mansoni. These findings offer a potential reference for the diagnosis and prevention of sparganosis.</p>","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"33 ","pages":"26"},"PeriodicalIF":2.4,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13104597/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147777957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trichinella spiralis serine proteinase enhances antibody-dependent cell-mediated (ADCC) killing of newborn larvae by driving M1 polarization via the NF-κB pathway.","authors":"Jin Yi Wu, Ru Zhang, Yao Zhang, Xin Zhuo Zhang, Dong Mei Xu, Ruo Dan Liu, Shao Rong Long, Zhong Quan Wang, Jing Cui","doi":"10.1051/parasite/2026020","DOIUrl":"10.1051/parasite/2026020","url":null,"abstract":"<p><p>Previous studies have shown that recombinant Trichinella spiralis serine proteinase (rTsSPc) promoted larval invasion of the gut epithelium, but its regulatory role on macrophage polarization is not clear. Immunofluorescence assay (IFA) confirmed specific rTsSPc's binding to RAW264.7 macrophages. The results of qPCR, Western blot, ELISA, and flow cytometry showed that rTsSPc significantly upregulated M1 macrophage markers (iNOS and CD86) and pro-inflammatory cytokines (TNF-α and IL-6), but not M2 markers (Arg1, CD206) and anti-inflammatory cytokines (IL-10, TGF-β). However, intestinal infective larvae (IIL) excretory-secretory antigens (ESAs) induced M2 polarization. Western blot revealed that rTsSPc activated the classical NF-κB pathway, as evidenced by increased phosphorylation levels of IKKβ, IκB-α, and NF-κB p65. Pretreatment of macrophages with NF-κB inhibitor pyrrolidine dithiocarbamate (PDTC) effectively suppressed rTsSPc-induced M1 polarization, decreased pro-inflammatory cytokine secretion, and reduced nitric oxide (NO) production. Functionally, rTsSPc-induced M1 polarization significantly enhanced antibody-dependent cell-mediated cytotoxicity (ADCC) of macrophages killing newborn larvae, but PDTC pretreatment resulted in a 41.62% reduction in cytotoxicity. Our results showed that rTsSPc bound specifically to macrophages and induced M1 polarization by activating the classical NF-κB pathway, thereby enhancing macrophage-mediated ADCC killing of newborn larvae. The findings indicated that TsSPc might strengthen host protective immunity via ADCC killing of larvae, and TsSPc may be considered a potential candidate antigen for developing anti-Trichinella vaccines.</p>","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"33 ","pages":"21"},"PeriodicalIF":2.4,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13088810/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147699370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling the tick-borne pathogens from domestic ruminant ticks in Malawi and the emergence of the brown ear tick in the southern region: implications for East Coast fever control.","authors":"Boniface Chikufenji, Uday Kumar Mohanta, Elisha Chatanga, Dallion Stopher, Onur Ceylan, Rika Umemiya-Shirafuji, Oriel Thekisoe, Madalitso Nkhata, Tatsuki Sugi, Junya Yamagishi, Xuenan Xuan, Kyoko Hayashida","doi":"10.1051/parasite/2026022","DOIUrl":"10.1051/parasite/2026022","url":null,"abstract":"<p><p>Ticks transmit a wide range of protozoan, bacterial, and viral pathogens to humans and animals globally. However, data on ticks infesting domestic ruminants and the pathogens they carry are scarce in Malawi. In this study, we examined ticks collected from domestic ruminants and screened them for selected veterinary and medically important protozoan and bacterial pathogens. A total of 964 ticks were collected from 202 cattle, 63 goats, and 16 sheep across eleven districts in Malawi. Ticks were morphologically identified to species level using taxonomical keys, with molecular confirmation by PCR amplification and sequencing of the 12S ribosomal RNA (12S rDNA) and cytochrome c oxidase subunit I (COI) genes. Tick DNA was further screened for tick-borne pathogens using species-specific PCR assays. Identified tick species included Rhipicephalus microplus (30.5%), Rhipicephalus appendiculatus (23.3%), Rhipicephalus decoloratus (13.2%), Rhipicephalus evertsi (9.8%), Hyalomma rufipes (7.5%), Amblyomma variegatum (6.3%), Rhipicephalus sanguineus sensu lato (tropical lineage) (3.6%), Hyalomma truncatum (2.8%), Rhipicephalus simus (2.0%), Rhipicephalus pravus (0.6%), and Rhipicephalus annulatus (0.4%). Overall, 37.0% of ticks carried at least one tick-borne pathogen, with Theileria parva being the most prevalent (34.7%), followed by Anaplasma marginale (17.4%), Babesia bigemina (14.9%), Anaplasma ovis (11.2%), Ehrlichia ruminantium (9.2%), Theileria mutans (8.4%), Babesia bovis (2.2%), and Anaplasma bovis (2.0%). This study provides the first molecular identification of ticks infesting domestic ruminants in Malawi and documents associated tick-borne pathogens. Notably, Rhipicephalus appendiculatus was identified for the first time in southern Malawi, refining current understanding of East Coast fever epidemiology and highlighting the need for updated surveillance approaches.</p>","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"33 ","pages":"22"},"PeriodicalIF":2.4,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13088809/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147699460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of historic and novel data reveals higher contemporary diversity of trematode metacercariae in freshwater fish.","authors":"Olena Kudlai, Rasa Binkienė, Vytautas Rakauskas, Nathan Jay Baker","doi":"10.1051/parasite/2025067","DOIUrl":"10.1051/parasite/2025067","url":null,"abstract":"<p><p>Trematode metacercariae are the most abundant and frequently encountered helminths in freshwater fish. Yet, accurate species identification remains challenging, potentially leading to an underestimation of trematode diversity. Using data from parasitological examinations of 1,030 fish (47 species) collected from diverse freshwater habitats in Lithuania (2022-2024), we assessed the contemporary diversity of trematode metacercariae, host associations, microhabitat preferences, and changes in diversity patterns and transmission dynamics. Through integrated morphological and molecular techniques, we identified metacercariae belonging to 51 species from eight families, more than doubling previously reported diversity (25 species). While trematode family composition remained largely unchanged - the Diplostomidae and Strigeidae remained the most diverse families - notable differences were observed at the species level. Metacercariae of the Echinochasmidae and Echinostomatidae were detected for the first time, while previously reported Clinostomidae were absent. Fish of the Leuciscidae hosted the highest trematode diversity. Host specificity of metacercariae was generally low, with most species being euryxenous. At the microhabitat level, eyes harboured the highest number of species, while muscles showed the highest metacercarial density. Notably, we detected species first genetically characterised in North America (Echinoparyphium sp. 2 and Ichthyocotylurus sp. 2) and species potentially belonging to the genus Neogogatea, previously known only from Asia and North America, highlighting potential invasion risks and suggesting that European trematode diversity remains substantially underestimated. Future efforts should obtain molecular data from correctly identified adult specimens to resolve the identity of species currently identified only to the genus or family level, thereby enabling assessment of their geographical distributions and ecological roles.</p>","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"33 ","pages":"1"},"PeriodicalIF":2.4,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12779263/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145918161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diversity and abundance of Culicoides on goat and cattle farms in the southern part of the Republic of Korea.","authors":"Seung Bak An, Jiseung Jeon, Jihun Ryu, Jong-Uk Jeong, In-Soon Roh, Kwang Shik Choi","doi":"10.1051/parasite/2026005","DOIUrl":"10.1051/parasite/2026005","url":null,"abstract":"<p><p>Biting midges of the genus Culicoides Latreille (Ceratopogonidae) pose a significant threat to veterinary health as vectors of over 60 viruses, most of which affect livestock. In this study, we used light traps to sample Culicoides populations on cattle and goat farms from May to October 2023 at 15 sites in Gyeongsangnam-do, Jeollanam-do, and Jeju Island, South Korea. Diversity and abundance were analysed based on the collection date, environmental conditions, and host species. A total of 124,055 individuals were collected, comprising 14 previously recorded and two newly recorded species: C. asiana and C. palawanensis. The dominant species was C. arakawae, which accounted for 80.60% of the total collected individuals, followed by C. punctatus (10.25%), and C. tainanus (3.36%), while the remaining 13 species constituted 5.80% of the collection. Total Culicoides abundance peaked in August (40.15%), driven largely by fluctuations in C. arakawae abundance, but the seasonal abundances of individual species varied. Culicoides arakawae and C. punctatus were dominant on the mainland, while C. matsuzawai, C. lungchiensis, and C. tainanus were dominant on Jeju Island. The dominant species on cattle farms were C. arakawae and C. punctatus, while C. arakawae dominated in collections from goat farms. The detection of two new species records suggests that the fauna of South Korea is still incompletely understood.</p>","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"33 ","pages":"6"},"PeriodicalIF":2.4,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12888750/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146150212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}