Parasite最新文献_第3页

Diversity of biting midges, mosquitoes and sand flies at four dog shelters in rural and peri-urban areas of Central Morocco. 摩洛哥中部农村和城郊地区四个狗收容所中咬螨、蚊子和沙蝇的多样性。

IF 2.3 2区医学

Parasite Pub Date : 2024-01-01 Epub Date: 2024-09-27 DOI: 10.1051/parasite/2024057

Abderrahmane Zahri, Mehdi Ahlamine, Fatima-Zahra Abou-Elaaz, Hasnaa Talimi, Ikhlass El Berbri, Thomas Balenghien, Maria Bourquia

{"title":"Diversity of biting midges, mosquitoes and sand flies at four dog shelters in rural and peri-urban areas of Central Morocco.","authors":"Abderrahmane Zahri, Mehdi Ahlamine, Fatima-Zahra Abou-Elaaz, Hasnaa Talimi, Ikhlass El Berbri, Thomas Balenghien, Maria Bourquia","doi":"10.1051/parasite/2024057","DOIUrl":"https://doi.org/10.1051/parasite/2024057","url":null,"abstract":"Blood-feeding arthropods are involved in the transmission of several pathogens that have a major impact on public health. Entomological investigations highlighted the composition, abundance, and diversity of flying hematophagous arthropods at four dog shelters located in central Morocco during an eight-month study, with the aim of discussing their vectorial roles and assessing the risk of these shelters as foci for zoonotic diseases. Monitoring of the arthropod fauna for 64 catch nights resulted in the collection of 2,321 biting midges (Ceratopogonidae), 570 mosquitoes (Culicidae), and 475 sand flies (Psychodidae). Fourteen Culicoides species were recorded and dominant species were Culicoides imicola (55.96%), C. paolae (16.07%), C. circumscriptus (10.29%), and C. newsteadi (5.77%). Three mosquito species were collected, including Culex pipiens s.l. (96.84%), Culiseta longiareolata (2.80%), and Cx. perexiguus (0.36%). Ten sand fly species were collected, including seven Phlebotomus species (62.70%) and three Sergentomyia species (37.30%); Sergentomyia minuta was the most dominant species (34.31%), followed by Phlebotomus sergenti (32.42%), typical Ph. perniciosus (8.63%), Ph. alexandri (6.94%), and Ph. riouxi (6.52%). The coexistence of several vectors in these study areas indicates the potential circulation of a wide range of pathogens, including zoonotic ones, thus requiring the implementation of surveillance and control programs to prevent the emergence and spread of disease outbreaks.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11433837/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142351526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular prevalence and subtype distribution of Blastocystis spp. among children who have diarrheia or are asymptomatic in Wenzhou, Zhejiang Province, China. 中国浙江省温州市腹泻或无症状儿童中子囊虫的分子流行率和亚型分布。

IF 2.9 2区医学

Parasite Pub Date : 2024-01-01 Epub Date: 2024-03-06 DOI: 10.1051/parasite/2024012

Wei Zhao, Guangxu Ren, Long Wang, Lisha Xie, Jiayang Wang, Jialiang Mao, Yanbin Sun, Gang Lu, Huicong Huang

{"title":"Molecular prevalence and subtype distribution of Blastocystis spp. among children who have diarrheia or are asymptomatic in Wenzhou, Zhejiang Province, China.","authors":"Wei Zhao, Guangxu Ren, Long Wang, Lisha Xie, Jiayang Wang, Jialiang Mao, Yanbin Sun, Gang Lu, Huicong Huang","doi":"10.1051/parasite/2024012","DOIUrl":"10.1051/parasite/2024012","url":null,"abstract":"Blastocystis sp., a significant zoonotic parasite with a global distribution, was the focus of this study, which aimed to investigate its prevalence and genetic diversity among diarrheic and asymptomatic children in Wenzhou, China. We collected 1,032 fecal samples from Yuying Children's Hospital, Wenzhou, China, comprising 684 from children with diarrhea and 348 from asymptomatic children. Genomic DNA extracted from these samples was used to detect Blastocystis spp. by PCR, targeting the small subunit ribosomal RNA gene. Subsequently, a phylogenetic tree was constructed, applying the maximum likelihood method. Blastocystis spp. were detected in 67 (6.5%) of the fecal samples. The prevalence rate of Blastocystis spp. in diarrheic children (8.8%; 60/684) was significantly higher than that in asymptomatic children (2.0%; 7/348) (χ 2 = 17.3, p < 0.001). Sequence analysis of the SSU rRNA gene identified five known Blastocystis spp. subtypes, ST1 (n = 12), ST2 (n = 5), ST3 (n = 35), ST4 (n = 12), and ST7 (n = 3). ST1 and ST3 were present in both diarrheic and asymptomatic children, while ST2, ST4, and ST7 were exclusive to diarrheic children. Intra-subtype genetic polymorphisms were identified, comprising four variations in ST1 (ST1-1 to ST1-4), five in ST3 (ST3-1 to ST3-5), two in ST4 (ST4-1 and ST4-2), and two in ST7 (ST7-1 and ST7-2). Notably, ST1-2 to ST1-4, ST3-3 to ST3-5, and ST7-1 and ST7-2 represent newly identified variations. The composition and genetic characteristics of subtypes among children in this region suggest various sources of infection, including human-to-human and animal-to-human transmission.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10918642/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140050108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Small intestinal flukes of the genus Metagonimus (Digenea: Heterophyidae) in Europe and the Middle East: A review of parasites with zoonotic potential. 欧洲和中东的Metagonimus属小肠吸虫（Digenea: Heterophyidae）：具有人畜共患病潜能的寄生虫综述。

IF 2.9 2区医学

Parasite Pub Date : 2024-01-01 Epub Date: 2024-03-29 DOI: 10.1051/parasite/2024016

Tomáš Scholz, Roman Kuchta, Daniel Barčák, Gábor Cech, Mikuláš Oros

{"title":"Small intestinal flukes of the genus Metagonimus (Digenea: Heterophyidae) in Europe and the Middle East: A review of parasites with zoonotic potential.","authors":"Tomáš Scholz, Roman Kuchta, Daniel Barčák, Gábor Cech, Mikuláš Oros","doi":"10.1051/parasite/2024016","DOIUrl":"10.1051/parasite/2024016","url":null,"abstract":"The heterophyid trematode Metagonimus romanicus (Ciurea, 1915) (Digenea) is redescribed on the basis of type material from domestic dogs (Canis familiaris) in Romania, vouchers from experimentally infected cats (Felis catus) and adults recovered from golden hamsters (Mesocricetus auratus) infected with metacercariae from scales of chub (Squalius cephalus) and common nase (Chondrostoma nasus) (Cypriniformes: Leuciscidae) in Hungary. This trematode, endemic to Europe and neighbouring regions (northwestern Türkiye), was previously misidentified as M. yokogawai (Katsurada, 1912), a zoonotic parasite of humans in East Asia. However, the two species differ considerably both genetically and morphologically, e.g., in the position of the ventral sucker, the presence of the prepharynx, the anterior extent of the vitelline follicles and the posterior extent of the uterus. Metagonimus ciureanus (Witenberg, 1929) (syn. Dexiogonimus ciureanus Witenberg, 1929), described from domestic cats and dogs in Israel, is a valid species distributed in the Middle East and Transcaucasia, which is also confirmed by molecular data. It differs from all Metagonimus species, including M. romanicus, in having symmetrical testes instead of the oblique testes of the other congeners. The zoonotic significance of M. romanicus and M. ciureanus is unclear, but appears to be low in Europe, mainly because raw or undercooked, whole fish with scales are generally not consumed. Accidental infection of fishermen by metacercariae in the scales when cleaning fish is more likely, but has never been reported. Remains of cyprinoids with scales infected with metacercariae of Metagonimus spp. can be an important natural source of infection for dogs, cats, and other carnivores, which can serve as a reservoir for these parasites.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10979786/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140318850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Asymptomatic Leishmania infantum infection in dogs and dog owners in an endemic area in southeast France. 法国东南部一个流行地区的狗和狗主人无症状幼年利什曼原虫感染。

IF 2.9 2区医学

Parasite Pub Date : 2024-01-01 Epub Date: 2024-03-26 DOI: 10.1051/parasite/2024019

Mallorie Hide, Gregory Michel, Kevin Legueult, Raphaelle Pin, Susana Leonard, Loïc Simon, Anne-Laure Bañuls, Pascal Delaunay, Pierre Marty, Christelle Pomares

{"title":"Asymptomatic Leishmania infantum infection in dogs and dog owners in an endemic area in southeast France.","authors":"Mallorie Hide, Gregory Michel, Kevin Legueult, Raphaelle Pin, Susana Leonard, Loïc Simon, Anne-Laure Bañuls, Pascal Delaunay, Pierre Marty, Christelle Pomares","doi":"10.1051/parasite/2024019","DOIUrl":"10.1051/parasite/2024019","url":null,"abstract":"The prevalence of asymptomatic leishmaniasis in dogs and their owners in the main endemic areas of France has not been studied to date. The objective of this study was to quantify asymptomatic Leishmania infantum infection in southeast France in healthy people and their dogs using molecular and serological screening techniques. We examined the presence of parasitic DNA using specific PCR targeting kinetoplast DNA (kDNA) and specific antibodies by serology (ELISA for dogs and Western blot for humans) among immunocompetent residents and their dogs in the Alpes-Maritimes. Results from 343 humans and 607 dogs were included. 46.9% (n = 161/343) of humans and 18.3% (n = 111/607) of dogs were PCR positive; 40.2% of humans (n = 138/343) and 9.9% of dogs (n = 60/607) were serology positive. Altogether, 66.2% of humans (n = 227) and 25.7% of dogs (n = 156) had positive serologies and/or positive PCR test results. Short-haired dogs were more frequently infected (71.8%, n = 112) than long-haired dogs (12.2%, n = 19) (p = 0.043). Dogs seemed to be more susceptible to asymptomatic infection according to their breed types (higher infection rates in scenthounds, gun dogs and herding dogs) (p = 0.04). The highest proportion of dogs and human asymptomatic infections was found in the Vence Region, corresponding to 28.2% (n = 20/71) of dogs and 70.5% (n = 31/44) of humans (4.5/100,000 people). In conclusion, the percentage of infections in asymptomatic humans is higher than in asymptomatic dogs in the studied endemic area. It is questionable whether asymptomatic infection in humans constitutes a risk factor for dogs.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10964848/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140288713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Geoepidemiology, seroprevalence and factors associated with Toxoplasma gondii infection in domicilied cats from Paraíba (Brazil). 帕拉伊巴州（巴西）家猫的地质流行病学、血清流行率以及与弓形虫感染相关的因素。

IF 2.9 2区医学

Parasite Pub Date : 2024-01-01 Epub Date: 2024-05-17 DOI: 10.1051/parasite/2024017

Ana Letícia Pereira Fernandes, Mariana de Melo Alves, Jordania Oliveira Silva, Ividy Bison, Ariana de Castro Tavares Silva, Roberta Nunes Parentoni, Jose Romulo Soares Dos Santos, Thais Ferreira Feitosa, Vinícius Longo Ribeiro Vilela, Arthur Willian de Lima Brasil

{"title":"Geoepidemiology, seroprevalence and factors associated with Toxoplasma gondii infection in domicilied cats from Paraíba (Brazil).","authors":"Ana Letícia Pereira Fernandes, Mariana de Melo Alves, Jordania Oliveira Silva, Ividy Bison, Ariana de Castro Tavares Silva, Roberta Nunes Parentoni, Jose Romulo Soares Dos Santos, Thais Ferreira Feitosa, Vinícius Longo Ribeiro Vilela, Arthur Willian de Lima Brasil","doi":"10.1051/parasite/2024017","DOIUrl":"https://doi.org/10.1051/parasite/2024017","url":null,"abstract":"Toxoplasma gondii is a parasite responsible for toxoplasmosis, an emerging and often neglected zoonosis in South America, particularly Brazil. Felines, the only definitive hosts, excrete oocysts in their feces, potentially infecting all homeotherms. Domestic cats are primarily responsible for contaminating human environments with these oocysts. Monitoring their populations is therefore essential to ensure proper toxoplasmosis prophylaxis. The aim of this study was to estimate the prevalence of T. gondii and exposure factors in a population of owner cats in the city of João Pessoa, Paraíba, Brazil. A total of 267 blood samples were collected from domestic cats aged between 1 and 15 years and tested with an immunofluorescence antibody test. The seroprevalence of antibodies against T. gondii was only 17.22% (46/267 individuals). This result therefore suggests a low contribution of domestic cats to T. gondii contamination of the urban environment. The cats' age and living environment were identified as risk factors for cat exposure to T. gondii.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11101201/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140958701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Population genetic structure of Schistosoma bovis and S. curassoni collected from cattle in Mali. 从马里牛身上采集的牛血吸虫和库拉索尼血吸虫的种群遗传结构。

IF 2.3 2区医学

Parasite Pub Date : 2024-01-01 Epub Date: 2024-07-02 DOI: 10.1051/parasite/2024035

Assitan Diakité, Privat Agniwo, Abdoulaye Dabo, Bakary Sidibé, Boris A E S Savassi, Ahristode Akplogan, Hassim Guindo, Laurent Dembélé, Moudachirou Ibikounlé, Safiatou Doumbo Niaré, Saidou Tembely, Jérôme Boissier

{"title":"Population genetic structure of Schistosoma bovis and S. curassoni collected from cattle in Mali.","authors":"Assitan Diakité, Privat Agniwo, Abdoulaye Dabo, Bakary Sidibé, Boris A E S Savassi, Ahristode Akplogan, Hassim Guindo, Laurent Dembélé, Moudachirou Ibikounlé, Safiatou Doumbo Niaré, Saidou Tembely, Jérôme Boissier","doi":"10.1051/parasite/2024035","DOIUrl":"10.1051/parasite/2024035","url":null,"abstract":"Schistosomiasis is of medical and veterinary importance. Despite the critical situation of schistosomiasis in sub-Saharan Africa, few molecular epidemiological studies have been carried out to determine the role of animals in its transmission. In Mali, it has been over three decades since the last molecular study of animal schistosomes was carried out. It is now urgent to identify circulating strains of the parasite because of potential interactions with other schistosome species, which could complicate disease control. The aim of our work was to study the composition and genetic structure of schistosome populations collected from cattle. The prevalence of schistosome was 23.9%, with the prevalences of Schistosoma bovis (Sb) and S. curassoni (Sc) estimated at 12.6% and 9.8%, respectively. No hybrid strains or S. haematobium were found. The parasites displayed distinct geographical distribution with Sb dominant in Bamako (78.8% and 98% in Central Bamako Slaughterhouse and Sabalibougou Slaughterhouses, respectively) and Sc dominant in Kayes (95.3%). Of the 476 parasites with a complete genetic profile, 60.4% were pure Sc, and were mainly from Kayes. We identified two clusters at the site level (Fst of 0.057 and 0.042 for Sb and Sc, respectively). Cluster 1 was predominantly composed of pure Sb parasites and cluster 2 was mainly composed of pure Sc parasites, from Bamako and Kayes, respectively. Our study shows that cattle schistosomiasis remains endemic in Mali with S. bovis and S. curassoni. A robust genetic structure between the different schistosome populations was identified, which included two clusters based on the geographical distribution of the parasites.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11218738/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141492976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prevalence and assemblage identified of Giardia duodenalis in zoo and farmed Asiatic black bears (Ursus thibetanus) from the Heilongjiang and Fujian Provinces of China. 中国黑龙江省和福建省动物园和养殖的亚洲黑熊（Ursus thibetanus）中十二指肠贾第鞭毛虫的流行率和聚集鉴定。

IF 2.3 2区医学

Parasite Pub Date : 2024-01-01 Epub Date: 2024-08-29 DOI: 10.1051/parasite/2024048

Jiani Chen, Liyuting Zhou, Wenjie Cao, Junchen Xu, Kuai Yu, Ting Zhang, Yiqing Wang, Jiayan Wang, Huicong Huang, Wei Zhao

{"title":"Prevalence and assemblage identified of Giardia duodenalis in zoo and farmed Asiatic black bears (Ursus thibetanus) from the Heilongjiang and Fujian Provinces of China.","authors":"Jiani Chen, Liyuting Zhou, Wenjie Cao, Junchen Xu, Kuai Yu, Ting Zhang, Yiqing Wang, Jiayan Wang, Huicong Huang, Wei Zhao","doi":"10.1051/parasite/2024048","DOIUrl":"10.1051/parasite/2024048","url":null,"abstract":"Captive and free-living wildlife serve as significant hosts for Giardia duodenalis. Asiatic black bears, valued for their economic and medicinal importance, are extensively farmed in China and also prevalent in zoos. However, studies on G. duodenalis in these animals in China are limited. Here, 218 feces samples of Asiatic black bears were collected: 36 from a zoo in Heilongjiang Province, and 182 from a farm in Fujian Province. Nested PCR of the SSU rRNA gene, followed by sequencing, was employed to determine the frequency and assemblage distribution of G. duodenalis. Positive samples underwent further analysis through multilocus genotyping (MLG) by amplifying the genes for glutamate dehydrogenase (gdh), β-giardin (bg), and triosephosphate isomerase (tpi). Of the 218 samples, G. duodenalis was detected in 22 cases at the SSU rRNA gene locus, including three from Heilongjiang and 19 from Fujian. Three assemblages were identified: A (n = 1), B (n = 16), and E (n = 2) in Fujian; and B (n = 3) in Heilongjiang. Out of the 22 positive samples, 20, 19, and 9 were effectively amplified and sequenced across the tpi, gdh, and bg loci, respectively. Seven samples were genotyped successfully at all three loci, identifying MLG-B1 (n = 1), MLG-B2 (n = 1), and MLG-B3 (n = 1), MLG-B4 (n = 1), MLG-B5 (n = 2), and MLG-B6 (n = 1) as the six assemblage B MLGs. This study marks the first documentation of G. duodenalis in Asiatic black bears in captivity in Fujian and Heilongjiang. The identification of zoonotic assemblages A and B, along with E, underscores potential public health concerns.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11363899/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142110553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Alobophora sandrae n. gen. n. sp. (Digenea: Caballerotrematidae) infecting Arapaima gigas sensu lato (Osteoglossiformes: Arapaimidae) with a revision of Caballerotrema, key to Caballerotrematidae, and updated phylogeny. Alobophora sandrae n. gen. n. sp. (Digenea: Caballerotrematidae) infecting Arapaima gigas sensu lato (Osteoglossiformes: Arapaimidae) with a revision of Caballerotrema, key to Caballerotrematidae, and updated phylogeny.

IF 2.3 2区医学

Parasite Pub Date : 2024-01-01 Epub Date: 2024-09-23 DOI: 10.1051/parasite/2024054

Kamila Cajiao-Mora, John H Brule, Micah B Warren, Steven P Ksepka, Haley R Dutton, Stephen A Bullard

{"title":"Alobophora sandrae n. gen. n. sp. (Digenea: Caballerotrematidae) infecting Arapaima gigas sensu lato (Osteoglossiformes: Arapaimidae) with a revision of Caballerotrema, key to Caballerotrematidae, and updated phylogeny.","authors":"Kamila Cajiao-Mora, John H Brule, Micah B Warren, Steven P Ksepka, Haley R Dutton, Stephen A Bullard","doi":"10.1051/parasite/2024054","DOIUrl":"10.1051/parasite/2024054","url":null,"abstract":"We propose and describe Alobophora sandrae Cajiao-Mora & Bullard n. gen., n. sp. (Digenea: Caballerotrematidae) for specimens we collected from arapaima, Arapaima gigas sensu lato (Osteoglossiformes: Arapaimidae) in the Amazon River near Leticia, Colombia. Alobophora differs from Caballerotrema Prudhoe, 1960 by lacking head collar projections and by having clustered corner spines and a narrow head collar (4-5× wider than pharynx), whereas Caballerotrema has head collar projections, lacks clustered corner spines, and has a broad head collar (7-8× wider than pharynx). We reassign Caballerotrema annulatum (Diesing, 1850) Ostrowski de Núñez & Sattmann, 2002 to the new genus, as Alobophora annulata (Diesing, 1850) Cajiao-Mora and Bullard n. comb., and provide a supplemental description of Caballerotrema brasiliense Prudhoe, 1960 based on specimens we collected from arapaima. We also examined the holotype and a paratype of Caballerotrema piscicola (Stunkard, 1960) Kostadinova & Gibson, 2001 and concluded that C. piscicola is a junior subjective synonym of C. brasiliense. Our 28S phylogeny recovered A. sandrae sister to A. annulata, with that clade sister to a clade comprising C. brasiliense and an innominate species of Caballerotrema. Caballerotrematidae was recovered sister to Echinostomatidae. We also provide a dichotomous key to caballerotrematids based on head collar projections, corner spine arrangement, proportional pharynx and head collar breadth, testes shape and arrangement, body surface spine shape and distribution, vitellarium distribution, and abundance of prostatic cells.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11418392/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142292837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biological characteristics and functions of a novel glutamate dehydrogenase from Trichinella spiralis. 螺旋毛癣菌新型谷氨酸脱氢酶的生物学特征和功能

IF 2.3 2区医学

Parasite Pub Date : 2024-01-01 Epub Date: 2024-10-28 DOI: 10.1051/parasite/2024065

Yong Kang Cheng, Yao Zhang, Zhao Yu Zhang, Pei Kun Cong, Ji Yu Feng, Ru Zhang, Shao Rong Long, Xi Zhang, Zhong Quan Wang, Jing Cui

{"title":"Biological characteristics and functions of a novel glutamate dehydrogenase from Trichinella spiralis.","authors":"Yong Kang Cheng, Yao Zhang, Zhao Yu Zhang, Pei Kun Cong, Ji Yu Feng, Ru Zhang, Shao Rong Long, Xi Zhang, Zhong Quan Wang, Jing Cui","doi":"10.1051/parasite/2024065","DOIUrl":"10.1051/parasite/2024065","url":null,"abstract":"Glutamate dehydrogenase (GDH) plays an important role in the metabolism of organisms. Its high abundance in mitochondria in particular highlights its core role in cellular physiological processes. GDH catalyzes the mutual conversion between L-glutamic acid and α-ketoglutaric acids. At the same time, this transformation is accompanied by the oxidation-reduction of NAD(H) or NADP(H). This process not only helps to link amino acid metabolism with sugar metabolism, but also helps maintain the balance of intracellular pH and nitrogen homeostasis. In this study, a novel Trichinella spiralis glutamate dehydrogenase (TsGDH) was cloned, expressed and identified. The results revealed that TsGDH was expressed at various stages of development of the nematode T. spiralis, with higher expression levels in the adult worm stage, and was mainly localized in the cuticle, muscular layer, stichosome and female intrauterine embryos. After RNAi treatment, larval natural TsGDH enzyme activity was obviously reduced, and metabolism, molting, growth and reproduction were also significantly inhibited. The results indicate that TsGDH plays an important role in the development and survival of T. spiralis, and it may be a potential molecular target of anti-Trichinella vaccines and drugs.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11514599/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142505373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Occurrence and genetic characterization of Enterocytozoon bieneusi in pet dogs in Yunnan Province, China. 中国云南省宠物狗肠虫病的发生和遗传特征。

IF 2.9 2区医学

Parasite Pub Date : 2024-01-01 Epub Date: 2024-05-24 DOI: 10.1051/parasite/2024025

Jinhua Jian, Jinrong Zi, Yaxue Wang, Yaming Yang, Yaxing Su, Lan Yao, Benfu Li, Xiaoxue Peng, Jianping Cao, Yujuan Shen, Aiqin Liu

{"title":"Occurrence and genetic characterization of Enterocytozoon bieneusi in pet dogs in Yunnan Province, China.","authors":"Jinhua Jian, Jinrong Zi, Yaxue Wang, Yaming Yang, Yaxing Su, Lan Yao, Benfu Li, Xiaoxue Peng, Jianping Cao, Yujuan Shen, Aiqin Liu","doi":"10.1051/parasite/2024025","DOIUrl":"10.1051/parasite/2024025","url":null,"abstract":"Enterocytozoon bieneusi is the most common microsporidian species in humans and can affect over 200 animal species. Considering possible increasing risk of human E. bieneusi infection due to close contact with pet dogs and identification of zoonotic E. bieneusi genotypes, 589 fresh fecal specimens of pet dogs were collected from Yunnan Province, China to determine the occurrence of E. bieneusi, characterize dog-derived E. bieneusi isolates, and assess their zoonotic potential at the genotype level. Enterocytozoon bieneusi was identified and genotyped by PCR and sequencing of the internal transcribed spacer (ITS) region of the ribosomal RNA (rRNA) gene. Twenty-nine specimens (4.9%) were positive. A statistical difference was observed in occurrence rates of E. bieneusi in pet dogs among 11 sampling sites by Fisher's exact test. Fifteen genotypes were identified and all of them phylogenetically belonged to zoonotic group 1, including four known genotypes (EbpC, D, Peru 8, and Henan-III) and 11 novel genotypes. Genotype Henan-III was reported in dogs for the first time. The finding of known genotypes found previously in humans and novel genotypes falling into zoonotic group 1 indicates that dogs may play a role in the transmission of E. bieneusi to humans in the investigated areas.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11120511/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141088281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0