Parasite最新文献_第3页

Effects of Trichinella spiralis excretory-secretory antigens on expression of indoleamine 2, 3-dioxygenase on dendritic cells in vitro. 旋毛虫排泄分泌抗原对体外树突状细胞吲哚胺2,3 -双加氧酶表达的影响。

IF 2.3 2区医学

Parasite Pub Date : 2025-01-01 Epub Date: 2025-04-16 DOI: 10.1051/parasite/2025018

Wenhao Yu, Xuhong Yuan, Peng Zhai, Xiaoyun Li, Caixia Han

{"title":"Effects of Trichinella spiralis excretory-secretory antigens on expression of indoleamine 2, 3-dioxygenase on dendritic cells in vitro.","authors":"Wenhao Yu, Xuhong Yuan, Peng Zhai, Xiaoyun Li, Caixia Han","doi":"10.1051/parasite/2025018","DOIUrl":"https://doi.org/10.1051/parasite/2025018","url":null,"abstract":"Indoleamine 2, 3-dioxygenase (IDO) is a potent immunoenzyme found in dendritic cells (DCs). Research has demonstrated that Trichinella spiralis induces IDO expression in the host immune response through its excretory-secretory (ES) antigens. However, the role of IDO in the immune response to T. spiralis remains unclear. To examine the effects of T. spiralis ES antigens on IDO expression in DCs in vitro, assessments were conducted using qRT-PCR, Western blotting (WB), flow cytometry, and siRNA transfer. The findings indicated that ES antigen stimulation upregulated IDO expression in DCs in vitro. Furthermore, ES antigen significantly enhanced the expression of the proinflammatory cytokines TNF-α and IFN-γ, along with the anti-inflammatory cytokine IL-10, downstream of IDO in DCs. Flow cytometry analysis confirmed that surface molecules CD40, MHC-II, CD80, and CD86 on DCs were upregulated following stimulation with ES antigen and lipopolysaccharide (LPS). Compared to the ES antigen alone, siRNA620 effectively inhibited IDO levels, demonstrating a statistically significant reduction. Continuous stimulation of DCs by ES antigens may lead to immune tolerance through the activation of IDO-mediated inflammation-associated factors. These results suggest that IDO expression in DCs plays a crucial role in T. spiralis infection.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"32 ","pages":"26"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12002673/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144027762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correlation between imaging features and rEm18 antibodies in alveolar echinococcosis: results from a multicenter study in France. 肺泡包虫病的影像学特征与rEm18抗体的相关性：来自法国一项多中心研究的结果

IF 2.3 2区医学

Parasite Pub Date : 2025-01-01 Epub Date: 2025-02-07 DOI: 10.1051/parasite/2024076

Gabriel Simon, Frédéric Grenouillet, Carine Richou, Eric Delabrousse, Oleg Blagoskonov, Anne Minello, Gerard Thiefin, Emilia Frentiu, Martine Wallon, Solange Bresson-Hadni, Paul Calame

{"title":"Correlation between imaging features and rEm18 antibodies in alveolar echinococcosis: results from a multicenter study in France.","authors":"Gabriel Simon, Frédéric Grenouillet, Carine Richou, Eric Delabrousse, Oleg Blagoskonov, Anne Minello, Gerard Thiefin, Emilia Frentiu, Martine Wallon, Solange Bresson-Hadni, Paul Calame","doi":"10.1051/parasite/2024076","DOIUrl":"10.1051/parasite/2024076","url":null,"abstract":"Background: To correlate imaging features of alveolar echinococcosis (AE) with the antibodies to recombinant Em18 (rEm18AB) at diagnosis and evaluate initial imaging features associated with serologic response, this retrospective study used data from the prospective multicenter EchinoVISTA study (NCT02876146). Imaging and serology were performed at diagnosis and during follow-up. Univariate and multivariate analyses were used to evaluate imaging features associated with the rEm18AB index. Follow-up analyses evaluated the imaging features associated with serologic response (defined as a 50% reduction in the baseline value within 2 years) in non-operated patients treated with albendazole alone.Results: From June 2012 to July 2016, 45 patients were included, with 8/45 (18%) having an rEm18AB index < 1. Maximum lesion size (76 mm [IQR = 57-93] vs. 36 mm [IQR = 26-51], p = 0.006), microcyst percentage (70% [IQR = 3-8] vs. 20% [IQR = 0.5-3.5], p = 0.004), and maximum standardized uptake value (SUV) on fluorodeoxyglucose positron emission tomography (5.1 [IQR = 4.4-6.2] vs. 2.6 [IQR = 2.4-3.9], p = 0.001) were associated with an rEm18AB index > 1. In patients treated with albendazole, serologic responders at 2 years had smaller lesions (5.3 [IQR = 3.8-72] vs. 3.5 [IQR = 2.7-3.7], p = 0.010) with less pedicle involvement, and lower initial rEm18AB index (2.98 ± 1.63 vs. 7.81 ± 3.95, p = 0.011).Conclusion: Maximum lesion size, percentage of microcysts within the lesion, and maximum lesion SUV are significant imaging features of AE correlated with the rEm18AB index. Serologic response at 2 years occurs primarily in patients with small lesions and a low rEm18AB index.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"32 ","pages":"8"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11804184/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143365559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular detection of parasites and host preference in wild-caught Culicoides biting midges (Diptera: Ceratopogonidae) in Chiang Mai and Nakhon Si Thammarat Provinces, Thailand. 泰国清迈和那空府野生库蠓（双翅目：蠓科）寄生虫分子检测及寄主偏好

IF 2.3 2区医学

Parasite Pub Date : 2025-01-01 Epub Date: 2025-01-22 DOI: 10.1051/parasite/2024082

Baby Kyi Soe, Saowalak Kaewmee, Chonlada Mano, Urassaya Pattanawong, Nopporn Tipparawong, Padet Siriyasatien, Derek Gatherer, Michael D Urbaniak, Paul A Bates, Narissara Jariyapan

{"title":"Molecular detection of parasites and host preference in wild-caught Culicoides biting midges (Diptera: Ceratopogonidae) in Chiang Mai and Nakhon Si Thammarat Provinces, Thailand.","authors":"Baby Kyi Soe, Saowalak Kaewmee, Chonlada Mano, Urassaya Pattanawong, Nopporn Tipparawong, Padet Siriyasatien, Derek Gatherer, Michael D Urbaniak, Paul A Bates, Narissara Jariyapan","doi":"10.1051/parasite/2024082","DOIUrl":"10.1051/parasite/2024082","url":null,"abstract":"Culicoides biting midges (Diptera: Ceratopogonidae) have been reported as potential vectors for haemoparasites. Information about host-vector-parasite specificity is required to confirm their status. Here, molecular detection of haemosporidians, Leishmania, trypanosomatids, and filarial nematodes in biting midges was conducted to understand their potential role as vectors, and their host preference was determined. Wild-caught biting midges were collected from six different localities of Chiang Mai and Nakhon Si Thammarat provinces, Thailand. A total of 6,578 individual Culicoides (170 males, 6,408 females) comprising 15 species of six sub-genera and two groups were collected. Also, 738 parous females and 29 engorged females were examined for parasites and host blood meals, respectively. Culicoides arakawae, C. mahasarakhamense, C. peregrinus and C. innoxius in Chiang Mai province, and C. innoxius and C. peregrinus in Nakhon Si Thammarat province were the most dominant species. Leucocytozoon spp., Leucocytozoon caulleryi and Plasmodium juxtanucleare were identified in five Culicoides species including C. mahasarakhamense, C. arakawae, C. oxystoma, C. fulvus, and C. guttifer. This study is the first record of L. caulleryi in the biting midge C. arakawae in Thailand. Blood meal analysis revealed that Culicoides primarily fed on cattle (17/29, 58.6%), followed by chickens (10/29, 34.5%), and humans (2/29, 6.9%). Our findings confirmed the existence of several Culicoides species in Thailand, which might be potential vectors for transmission of haemosporidians (Leucocytozoon and Plasmodium). Information from host blood meal analyses underlined their preference for large mammals, followed by domestic chickens. More anthropophilic Culicoides species remain to be discovered.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"32 ","pages":"2"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11752737/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143009449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Population structure of Clinostomum complanatum (Trematoda: Digenea) with new data on haplotype diversity of flukes from Slovakia and Italy. 斯洛伐克和意大利吸虫单倍型多样性的新数据及吸虫种群结构。

IF 2.3 2区医学

Parasite Pub Date : 2025-01-01 Epub Date: 2025-01-22 DOI: 10.1051/parasite/2024080

Ľudmila Juhásová, Eva Čisovská Bazsalovicsová, Monica Caffara, Alžbeta Radačovská, Andrea Gustinelli, Lucia Dinisová, Yaroslav Syrota, Ivica Králová-Hromadová

{"title":"Population structure of Clinostomum complanatum (Trematoda: Digenea) with new data on haplotype diversity of flukes from Slovakia and Italy.","authors":"Ľudmila Juhásová, Eva Čisovská Bazsalovicsová, Monica Caffara, Alžbeta Radačovská, Andrea Gustinelli, Lucia Dinisová, Yaroslav Syrota, Ivica Králová-Hromadová","doi":"10.1051/parasite/2024080","DOIUrl":"10.1051/parasite/2024080","url":null,"abstract":"The fluke Clinostomum complanatum, a parasite of piscivorous birds, but also reptiles and rarely mammals, has established several foci in the western Palaearctic regions. Previous studies pointed out the complicated taxonomy of the genus, but broader population genetic analysis of C. complanatum has not yet been carried out. The aim of this study was to determine the structure, intraspecific variability, and diversity of mitochondrial cox1 haplotypes of C. complanatum from different localities in Slovakia (Danube floodplain forests) and Italy (Emilia-Romagna and Tuscany), as well as to evaluate the interrelationships among populations from Europe, the Middle East, and North Africa. The genetic structure of C. complanatum from Slovakia and Italy was represented by a great number of haplotypes, showing stable populations with high intraspecific diversity. The haplotypes of samples from other localities (Romania, Turkey, Egypt, and Iran) showed possible gene flow among the populations from Central Europe down to the Mediterranean region, North Africa, and the Middle East. The genetic homogeneity of these samples can be linked to the distribution and migratory routes of the definitive hosts, aquatic piscivorous birds, mainly herons and cormorants, that spread parasite eggs among the continents.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"32 ","pages":"3"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11752738/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143009450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Challenges and lessons from a vector control campaign targeting Glossina palpalis palpalis in an isolated protected forest area in Abidjan, Côte d'Ivoire. 在阿比让（Côte科特迪瓦）一个孤立的森林保护区开展针对palpalis glssina palpalis palpalis病媒控制运动的挑战和经验教训。

IF 2.3 2区医学

Parasite Pub Date : 2025-01-01 Epub Date: 2025-04-15 DOI: 10.1051/parasite/2025017

Yao Jean Rodrigue Konan, Bi Tra Dieudonné Ta, Djakaridja Berté, Bamoro Coulibaly, Kinifo Donatien Coulibaly, Nick Steven Egnankon, Foungniguée Diarrassouba, Kouassi Albert Djabo, Stéphanie Watier-Grillot, Jean-Paul Demoncheaux, Koffi Alain De Marie Kouadio, Louis N'Dri, Philippe Solano, Sophie Ravel, Guy Pacôme Adingra, Antoine Barreaux, Adeline Ségard, Dramane Kaba, Vincent Jamonneau, Thierry De Meeûs, Vincent Djohan

{"title":"Challenges and lessons from a vector control campaign targeting Glossina palpalis palpalis in an isolated protected forest area in Abidjan, Côte d'Ivoire.","authors":"Yao Jean Rodrigue Konan, Bi Tra Dieudonné Ta, Djakaridja Berté, Bamoro Coulibaly, Kinifo Donatien Coulibaly, Nick Steven Egnankon, Foungniguée Diarrassouba, Kouassi Albert Djabo, Stéphanie Watier-Grillot, Jean-Paul Demoncheaux, Koffi Alain De Marie Kouadio, Louis N'Dri, Philippe Solano, Sophie Ravel, Guy Pacôme Adingra, Antoine Barreaux, Adeline Ségard, Dramane Kaba, Vincent Jamonneau, Thierry De Meeûs, Vincent Djohan","doi":"10.1051/parasite/2025017","DOIUrl":"https://doi.org/10.1051/parasite/2025017","url":null,"abstract":"Vector control (VC) is one of the strategies employed to manage African trypanosomoses. This study aimed at assessing the effectiveness of a VC campaign against Glossina palpalis palpalis using tiny targets (TTs) impregnated with insecticide in an isolated, protected forest in Abidjan, Côte d'Ivoire, while considering ecological, genetic, and operational factors. Between January 2020 and September 2022, 2,712 TTs were deployed at 684 sites, covering a total area of 1.7 km2. VC monitoring was conducted using Vavoua traps during 12 evaluation surveys, between June 2020 and March 2023. Five months after the initial TT deployment, tsetse fly density had decreased by 98.53%. Although tsetse density remained low due to TT redeployment and reinforcement, there was a significant increase a few months after the last redeployment. VC appeared to have minimal impact on the genetic structuring of G. p. palpalis. This suggested recruitment of local surviving tsetse flies all along the VC campaign due to a low probability of tsetse coming into contact with TTs, or to the evolution of behavioral or physiological resistance to control efforts. The genetic study revealed that one of the microsatellite markers used, the GPCAG locus, exhibited a selection signature possibly in response to VC. This could partly explain the challenges encountered in eliminating a seemingly isolated tsetse population thriving in a particularly favorable habitat.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"32 ","pages":"25"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12002674/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144019597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

No evidence of Alzheimer's disease pathology in mice infected with Toxocara canis. 在感染犬弓形虫的小鼠中没有阿尔茨海默病的病理证据。

IF 2.3 2区医学

Parasite Pub Date : 2025-01-01 Epub Date: 2025-04-09 DOI: 10.1051/parasite/2025019

Ondřej Vosála, Barbora Šmídová, Jan Novák, Jan Svoboda, Tomáš Petrásek, Iveta Vojtěchová, Tomáš Macháček

{"title":"No evidence of Alzheimer's disease pathology in mice infected with Toxocara canis.","authors":"Ondřej Vosála, Barbora Šmídová, Jan Novák, Jan Svoboda, Tomáš Petrásek, Iveta Vojtěchová, Tomáš Macháček","doi":"10.1051/parasite/2025019","DOIUrl":"https://doi.org/10.1051/parasite/2025019","url":null,"abstract":"The potential link between the infections and the development of Alzheimer's disease (AD) has led to speculations about the role of various pathogens in triggering amyloid-β (Aβ) overproduction, possibly leading to AD onset. The globally distributed dog roundworm Toxocara canis was suggested to be a suitable candidate due to neurotropism of the larvae and infection chronicity. This study investigated whether chronic T. canis infection induces AD-like pathology in mice and whether Aβ is toxic to T. canis. BALB/c and APP/PS1 transgenic mice, which overproduce Aβ, were infected with T. canis L3 larvae and monitored for larval burden, Aβ accumulation, and behavioral changes. In vitro tests of recombinant Aβ toxicity against the larvae were also performed. Despite the presence of T. canis larvae in the central nervous system 8 and 16 weeks post-infection, no significant increase in Aβ concentration or AD-related behavioral alterations were observed. Aβ was detected on the surface and within the intestines of T. canis larvae, but in vitro exposure to recombinant Aβ did not affect larval viability or morphology. Our findings suggest that T. canis infection does not trigger AD-like pathology in mice, and Aβ does not act as an antiparasitic agent. This challenges the emerging hypothesis that chronic neurotoxocarosis infections may contribute to AD development.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"32 ","pages":"24"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11987500/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144008598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Apoptotic proteins in Leishmania donovani: in silico screening, modeling, and validation by knock-out and gene expression analysis. 多诺瓦利什曼原虫的凋亡蛋白：通过敲除和基因表达分析筛选，建模和验证。

IF 2.3 2区医学

Parasite Pub Date : 2025-01-01 Epub Date: 2025-02-12 DOI: 10.1051/parasite/2024081

Ketan Kumar, Lucien Crobu, Rokhaya Thiam, Chandi C Mandal, Yvon Sterkers, Vijay Kumar Prajapati

{"title":"Apoptotic proteins in Leishmania donovani: in silico screening, modeling, and validation by knock-out and gene expression analysis.","authors":"Ketan Kumar, Lucien Crobu, Rokhaya Thiam, Chandi C Mandal, Yvon Sterkers, Vijay Kumar Prajapati","doi":"10.1051/parasite/2024081","DOIUrl":"10.1051/parasite/2024081","url":null,"abstract":"Visceral leishmaniasis, a life-threatening vector-borne illness that disproportionately affects children and elderly immunocompromised people, is a primary tropical neglected disease. No apoptotic partner proteins have yet been reported in Leishmania donovani, while their identification could contribute to knowledge on parasite cell death and the establishment of alternative therapeutics. We searched for mammalian Bcl-2 family protein orthologs and found one anti-apoptotic and two pro-apoptotic orthologs in L. donovani. A pro-death aquaporin protein, due to its characteristic BH3 domain known to interact with pro-apoptotic proteins in mammalian Bcl-2 family proteins, was also included in this study. Molecular docking and molecular dynamics simulations were conducted to assess protein-protein interactions between the identified apoptotic proteins and mimic mammalian intrinsic apoptotic pathways. The results showed that both pro-apoptotic proteins interacted with the hydrophobic pocket of the anti-apoptotic ortholog, forming a stable complex. This interaction may represent a critical event in an apoptotic pathway in L. donovani. To further characterise it, we used CRISPR-Cas9 approaches to target the identified proteins. Pure knocked population mutants, and episomal over-expressing mutant cells were exposed to apoptotic stimuli. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay and quantitative expression profiling suggested that these proteins are involved in the parasite's apoptosis and could play a role in its survival.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"32 ","pages":"9"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11825125/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143414805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Toxocara canis infection on liver and lung microbial flora diversity and composition in dogs. 犬弓形虫感染对犬肝脏和肺部微生物菌群多样性和组成的影响。

IF 2.3 2区医学

Parasite Pub Date : 2025-01-01 Epub Date: 2025-03-05 DOI: 10.1051/parasite/2025011

Na Wang, Soben Sieng, Tian Liang, Ping Chen, Jingyun Xu, Qian Han

{"title":"Effect of Toxocara canis infection on liver and lung microbial flora diversity and composition in dogs.","authors":"Na Wang, Soben Sieng, Tian Liang, Ping Chen, Jingyun Xu, Qian Han","doi":"10.1051/parasite/2025011","DOIUrl":"10.1051/parasite/2025011","url":null,"abstract":"Toxocariasis is a zoonotic parasitic disease that is widely prevalent in the world. Toxocara canis adults are parasitic in the small intestinal tract of canids, and the larvae migrate to the liver and lungs before reaching the final destination. Our previous experiments have confirmed that T. canis infection could affect the composition of host intestinal microbial flora. In this experiment, we further analyze the potential effects of T. canis infection on host liver and lung microbial flora. Utilizing 16s rRNA high-throughput sequencing, coupled with various bioinformatics analysis techniques, our study revealed that T. canis infection significantly elevated the abundance of certain opportunistic pathogens in the host's liver and lungs. This marked elevation contributes to the establishment of infection. Through cluster analysis, we found that the changes in the microbiota of the liver and lungs were independent of the microbial flora carried by T. canis adults. However, whether the changes are due to the migration of larvae remains to be explored. In short, T. canis infections have a significant impact on the abundance and diversity of flora in the host tissues, and the changes in microbiota abundance and diversity could further influence tissue homeostasis and immune responses, thus regulating the establishment of infection.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"32 ","pages":"17"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11882136/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143567842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular characterization of common zoonotic protozoan parasites and bacteria causing diarrhea in dairy calves in Ningxia Hui Autonomous Region, China. 导致中国宁夏回族自治区奶牛犊牛腹泻的常见人畜共患病原生动物寄生虫和细菌的分子特征。

IF 2.3 2区医学

Parasite Pub Date : 2024-01-01 Epub Date: 2024-10-01 DOI: 10.1051/parasite/2024059

Jia-Qi Zhao, Ying-Ying Fan, Yun-Duan Lei, Ding Liu, Jun-Wei Wang, Xin Yang, Jun-Ke Song, Guang-Hui Zhao

{"title":"Molecular characterization of common zoonotic protozoan parasites and bacteria causing diarrhea in dairy calves in Ningxia Hui Autonomous Region, China.","authors":"Jia-Qi Zhao, Ying-Ying Fan, Yun-Duan Lei, Ding Liu, Jun-Wei Wang, Xin Yang, Jun-Ke Song, Guang-Hui Zhao","doi":"10.1051/parasite/2024059","DOIUrl":"10.1051/parasite/2024059","url":null,"abstract":"Diarrhea caused by zoonotic pathogens is one of the most common diseases in dairy calves, threatening the health of young animals. Humans are also at risk, in particular children. To explore the pathogens causing diarrhea in dairy calves, the present study applied PCR-based sequencing tools to investigate the occurrence and molecular characteristics of three parasites (Cryptosporidium spp., Giardia duodenalis, and Enterocytozoon bieneusi) and three bacterial pathogens (Escherichia coli, Clostridium perfringens, and Salmonella spp.) in 343 fecal samples of diarrheic dairy calves from five farms in Lingwu County, Ningxia Hui Autonomous Region, China. The total positive rate of these pathogens in diarrheic dairy calves was 91.0% (312/343; 95% CI, 87.9-94.0), with C. perfringens (61.5%, 211/343; 95% CI, 56.3-66.7) being the dominant one. Co-infection with two to five pathogens was found in 67.3% (231/343; 95% CI, 62.4-72.3) of investigated samples. There were significant differences (p < 0.05) in the positive rates of Cryptosporidium spp. and diarrheagenic E. coli among farms, age groups, and seasons. Two Cryptosporidium species (C. parvum and C. bovis) and five gp60 subtypes of C. parvum (IIdA15G1, IIdA20G1, IIdA19G1, IIdA14G1, and a novel IIdA13G1) were identified. Two assemblages (assemblage E and zoonotic assemblage A) of G. duodenalis and six ITS genotypes of E. bieneusi (J, Henan-IV, EbpC, I, EbpA, and ESH-01) were observed. Four virulence genes (eaeA, stx1, stx2, and st) of diarrheagenic E. coli and one toxin type (type A) of C. perfringens were detected. Our study enriches our knowledge on the characteristics and zoonotic potential of diarrhea-related pathogens in dairy calves.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"31 ","pages":"60"},"PeriodicalIF":2.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11444552/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142366105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel gene arrangement in the mitochondrial genome of Aspersentis megarhynchus (Acanthocephala, Echinorhynchida, Heteracanthocephalidae), and its phylogenetic implications. Aspersentis megarhynchus（棘鱼科、棘鱼属、异棘鱼科）线粒体基因组中的新基因排列及其系统发育意义。

IF 2.3 2区医学

Parasite Pub Date : 2024-01-01 Epub Date: 2024-10-08 DOI: 10.1051/parasite/2024064

Yuan-Yuan Xie, Hui-Xia Chen, Tetiana A Kuzmina, Olga Lisitsyna, Liang Li

{"title":"Novel gene arrangement in the mitochondrial genome of Aspersentis megarhynchus (Acanthocephala, Echinorhynchida, Heteracanthocephalidae), and its phylogenetic implications.","authors":"Yuan-Yuan Xie, Hui-Xia Chen, Tetiana A Kuzmina, Olga Lisitsyna, Liang Li","doi":"10.1051/parasite/2024064","DOIUrl":"10.1051/parasite/2024064","url":null,"abstract":"The Heteracanthocephalidae Petrochenko, 1956 is a rare family of acanthocephalans mainly parasitic in fishes. The pattern of mitogenomic evolution of the Heteracanthocephalidae is still unknown, and the phylogenetic relationships of the Heteracanthocephalidae with the other 14 families within the order Echinorhynchida remain unclear. In the present study, the complete mitochondrial genome of Aspersentis megarhynchus (von Linstow, 1892) Golvan, 1960 was sequenced and annotated for the first time, which represents the first mitogenomic data for the genus Aspersentis and also for the family Heteracanthocephalidae. The mitogenome of A. megarhynchus has 14,661 bp and includes 36 genes, containing 12 protein-coding genes (PCGs) (missing atp8), 22 tRNA genes, and 2 ribosomal RNAs (rrnS and rrnL), plus two non-coding regions. Comparative mitochondrial genomic analysis revealed that the presence of translocations of several tRNA genes (trnV, trnE, and trnT) and the gene arrangement in the mitogenome of A. megarhynchus represents a new type in Acanthocephala. Moreover, the mitogenomic phylogenetic results based on concatenated amino acid sequences of 12 protein-coding genes strongly supported the validity of the Heteracanthocephalidae and suggested close affinity between the Heteracanthocephalidae and Echinorhynchidae in the order Echinorhynchida.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"31 ","pages":"63"},"PeriodicalIF":2.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11460826/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142392265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0