Parasite最新文献_第4页

Proliferation of MDSCs may indicate a lower ^CD4+ T cell immune response in schistosomiasis japonica. MDSCs 的增殖可能表明日本血吸虫病的 CD4+ T 细胞免疫反应较低。

IF 2.3 2区医学

Parasite Pub Date : 2024-01-01 Epub Date: 2024-08-29 DOI: 10.1051/parasite/2024050

Bo Peng, Yulin Luo, Shudong Xie, Quan Zhuang, Junhui Li, Pengpeng Zhang, Kai Liu, Yu Zhang, Chen Zhou, Chen Guo, Zhaoqin Zhou, Jie Zhou, Yu Cai, Meng Xia, Ke Cheng, Yingzi Ming

{"title":"Proliferation of MDSCs may indicate a lower CD4+ T cell immune response in schistosomiasis japonica.","authors":"Bo Peng, Yulin Luo, Shudong Xie, Quan Zhuang, Junhui Li, Pengpeng Zhang, Kai Liu, Yu Zhang, Chen Zhou, Chen Guo, Zhaoqin Zhou, Jie Zhou, Yu Cai, Meng Xia, Ke Cheng, Yingzi Ming","doi":"10.1051/parasite/2024050","DOIUrl":"10.1051/parasite/2024050","url":null,"abstract":"Background: Schistosoma japonicum (S. japonicum) is the main species of Schistosoma prevalent in China. Myeloid-derived suppressor cells (MDSCs) are important immunoregulatory cells and generally expand in parasite infection, but there is little research relating to MDSCs in Schistosoma infection.Methods: Fifty-six S. japonicum-infected patients were included in this study. MDSCs and percentages and absolute cell numbers of lymphocyte subsets, including CD3+ T cells, CD4+ T cells, CD8+ T cells, B cells and natural killer (NK) cells were detected using flow cytometry. The degree of liver fibrosis was determined using color Doppler ultrasound.Results: Patients infected with S. japonicum had a much higher percentage of MDSCs among peripheral blood mononuclear cells (PBMCs) than the healthy control. Regarding subpopulations of MDSCs, the percentage of granulocytic myeloid-derived suppressor cells (G-MDSCs) was clearly increased. Correlation analysis showed that the absolute cell counts of T-cell subsets correlated negatively with the percentages of MDSCs and G-MDSCs among PBMCs. The percentage of G-MDSCs in PBMCs was also significantly higher in patients with liver fibrosis diagnosed by color doppler ultrasound (grade > 0), and the percentage of G-MDSCs in PBMCs and liver fibrosis grading based on ultrasound showed a positive correlation.Conclusion: S. japonicum infection contributes to an increase in MDSCs, especially G-MDSCs, whose proliferation may inhibit the number of CD4+ T cells in peripheral blood. Meanwhile, there is a close relationship between proliferation of G-MDSCs and liver fibrosis in S. japonicum-infected patients.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"31 ","pages":"52"},"PeriodicalIF":2.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11363901/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142110554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of an accusation of a possible breach of ethics in an article published in Parasite. 对《寄生虫》杂志发表的一篇文章中可能存在的违反职业道德的指控进行分析。

IF 2.9 2区医学

Parasite Pub Date : 2024-01-01 Epub Date: 2024-05-15 DOI: 10.1051/parasite/2024022

引用次数: 0

Diversity of Babesia spp. in skunks from selected states in the United States of America. 美国部分州臭鼬中巴贝斯菌属的多样性。

IF 2.3 2区医学

Parasite Pub Date : 2024-01-01 Epub Date: 2024-07-24 DOI: 10.1051/parasite/2024043

Kayla B Garrett, Justin Brown, Mourad Gabriel, Robert Dowler, J Clint Perkins, Dianna Krejsa, Michael J Yabsley

{"title":"Diversity of Babesia spp. in skunks from selected states in the United States of America.","authors":"Kayla B Garrett, Justin Brown, Mourad Gabriel, Robert Dowler, J Clint Perkins, Dianna Krejsa, Michael J Yabsley","doi":"10.1051/parasite/2024043","DOIUrl":"10.1051/parasite/2024043","url":null,"abstract":"Babesia species are intraerythrocytic protozoan parasites that infect a variety of hosts. The goal of this study was to evaluate the piroplasm species present in skunks in various states in the United States and determine whether there was any geographic variation. Spleen, whole blood, or blood on filter paper were received from Pennsylvania, Kentucky, North Carolina, South Carolina, Georgia, Missouri, Louisiana, Texas, Kansas, and California, and were tested for Babesia sp. We tested four species of skunks including striped skunk (Mephitis mephitis, n = 72), eastern spotted skunk (Spilogale putorius, n = 28), western spotted skunk (Spilogale gracilis, n = 15), and hog-nosed skunk (Conepatus leuconotus, n = 11). A PCR assay targeting the 18S rRNA region and cox1 region were used to determine if skunks were infected with piroplasms and for phylogenetic analyses. A total of 48.4% (61/126) of skunks tested positive for a Babesia species. Both the 18S and cox1 analysis supported a skunk-specific Babesia microti-like sp. of carnivores as well as a species in the B. microti complex that is phylogenetically unique from both B. microti of humans and the B. microti-like sp. of carnivores. In the 18S analysis, there was a third species of Babesia in hog-nosed skunks in the western piroplasm group. This study shows that at least three species of piroplasms occur in skunk species in the United States and further highlights the importance of phylogenetic analyses and the use of multiple gene targets when studying piroplasms.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"31 ","pages":"42"},"PeriodicalIF":2.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11271706/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141760255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Host-specific monogeneans parasitizing freshwater fish: The ecology and evolution of host-parasite associations. 寄生于淡水鱼的宿主特异性单基因虫：宿主与寄生虫关系的生态学与进化。

IF 2.3 2区医学

Parasite Pub Date : 2024-01-01 Epub Date: 2024-10-03 DOI: 10.1051/parasite/2024058

Andrea Šimková

{"title":"Host-specific monogeneans parasitizing freshwater fish: The ecology and evolution of host-parasite associations.","authors":"Andrea Šimková","doi":"10.1051/parasite/2024058","DOIUrl":"10.1051/parasite/2024058","url":null,"abstract":"Monogenea (Platyhelminthes), mainly gill and fin ectoparasites of fish, are often recognized as host specific and morphologically and ecologically diverse. These parasites exhibit high species diversity at the level of host species or individual fish specimens. Using case studies, especially those widely performed in Dactylogyrus parasitizing cyprinoid fish, this article presents current knowledge on the ecology and evolution of congeneric gill monogeneans. The important aspects of the ecology of congeneric monogeneans are highlighted, in particular: host specificity expressed at several host levels (from strict specificity to phylogenetic specificity), microhabitat specificity expressed by restricted positions on fish gills to facilitate intraspecific mating, and the link between microhabitat preference and morphological adaptation (i.e., sclerotized structures of the haptor) or reproductive isolation. From the evolutionary perspective, this study focused on the processes of the speciation and diversification of congeneric monogeneans, highlighting the role of host switch as the most prominent coevolutionary event, accompanied in some cases mostly by intrahost speciation or cospeciation, as revealed by cophylogenetic studies. Here, important knowledge on evolutionary patterns of host specificity, microhabitat specificity, and morphological adaptation is presented. Host-specific monogeneans may represent an important tool for studying the historical biogeography of their hosts. Specifically, in the case of freshwater fish hosts exhibiting disjunctive distribution, they reflect both historical and contemporary contacts. The role of host-specific congeneric monogeneans in revealing historical intercontinental and intracontinental contacts between freshwater fish is highlighted. Finally, the importance of the role of genetic coadaptation, limiting the presence of host-specific monogeneans in hybrid fish, is emphasized.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"31 ","pages":"61"},"PeriodicalIF":2.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11449429/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142372534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficacy of a topical combination of esafoxolaner, eprinomectin and praziquantel against Amblyomma maculatum infestations in cats. 伊沙福柳胺、伊普拉菌素和吡喹酮外用复方制剂对猫斑疹伤寒的疗效。

IF 2.3 2区医学

Parasite Pub Date : 2024-01-01 Epub Date: 2024-08-07 DOI: 10.1051/parasite/2024044

Eric Tielemans, Carin Rautenbach, Zamantungwa Khumalo, Frederic Beugnet

{"title":"Efficacy of a topical combination of esafoxolaner, eprinomectin and praziquantel against Amblyomma maculatum infestations in cats.","authors":"Eric Tielemans, Carin Rautenbach, Zamantungwa Khumalo, Frederic Beugnet","doi":"10.1051/parasite/2024044","DOIUrl":"10.1051/parasite/2024044","url":null,"abstract":"Amblyomma maculatum, the Gulf Coast tick, infests a wide range of vertebrate species including livestock, dogs, cats, and humans. It is a species of significant veterinary and public health importance, especially as a vector of diseases, for instance American canine hepatozoonosis or tidewater spotted fever. An experimental study was conducted to evaluate the efficacy of NexGard® Combo, a topical endectoparasiticide product for cats combining eprinomectin, praziquantel and esafoxolaner, against induced infestations of A. maculatum in cats. This Good Clinical Practice (GCP) study used a randomized, negative controlled, masked design. Ten cats were allocated to an untreated group and ten to a treated group, dosed once on Day 0 at the minimum label dose. On Days -2, 7, 14, 21, 28, 35, and 42, cats were infested with ~50 unfed adult A. maculatum. On Days 3, 10, 17, 24, 31, 38, and 45, i.e., 72 h after treatment and subsequent infestations, ticks were removed, counted and the numbers of live attached tick in each group were used for efficacy calculations. At each time-point, all untreated cats were adequately infested, demonstrating a vigorous tick population and an adequate study model. The curative efficacy after a single application against existing tick infestation, 72 h after treatment, was 98.7%. The preventive efficacy, 72 h after weekly infestations, over the following five weeks ranged from 93.8% to 99.4%.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"31 ","pages":"44"},"PeriodicalIF":2.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11305115/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141897958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Radiation dose fractionation and its potential hormetic effects on male Glossina palpalis gambiensis (Diptera: Glossinidae): a comparative study of reproductive and flight quality parameters. 辐射剂量分馏及其对雄性Glossina palpalis gambiensis (Diptera: Glossinidae)的潜在激素效应：生殖和飞行质量参数的比较研究。

IF 2.9 2区医学

Parasite Pub Date : 2024-01-01 Epub Date: 2024-02-08 DOI: 10.1051/parasite/2024001

Bénéwendé Aristide Kaboré, Syeda Dua Taqi, Athumani Mkinga, Anibal E Morales Zambrana, Robert L Mach, Marc Jb Vreysen, Chantel J de Beer

{"title":"Radiation dose fractionation and its potential hormetic effects on male Glossina palpalis gambiensis (Diptera: Glossinidae): a comparative study of reproductive and flight quality parameters.","authors":"Bénéwendé Aristide Kaboré, Syeda Dua Taqi, Athumani Mkinga, Anibal E Morales Zambrana, Robert L Mach, Marc Jb Vreysen, Chantel J de Beer","doi":"10.1051/parasite/2024001","DOIUrl":"10.1051/parasite/2024001","url":null,"abstract":"One of the most critical factors for implementing the sterile insect technique for the management of tsetse is the production of large quantities of highly competitive sterile males in the field. Several factors may influence the biological quality of sterile males, but optimizing the irradiation protocols to limit unwanted somatic cell damage could improve male performance. This study evaluated the effect of fractionation of gamma radiation doses on the fertility and flight quality of male Glossina palpalis gambiensis. Induced sterility was assessed by mating irradiated males with virgin fertile females. Flight quality was assessed using a standard protocol. The male flies were irradiated as pupae on day 23-27 post larviposition with 110 Gy, either in a single dose or in fractionations of 10 + 100 Gy and 50 + 60 Gy separated by 1-, 2- and 3-day intervals or 55 + 55 Gy separated by 4-, 8-, and 24-hour intervals. All treatments induced more than 90% sterility in females mated with irradiated males, as compared with untreated males. No significant differences were found in emergence rate or flight propensity between fractionated and single radiation doses, nor between the types of fractionations. Overall, the 50(D0) + 60(D1) Gy dose showed slightly higher induced sterility, flight propensity, and survival of males under feeding regime. Dose fractionation resulted in only small improvements with respect to flight propensity and survival, and this should be traded off with the required increase in labor that dose fractionation entails, especially in larger control programs.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"31 ","pages":"4"},"PeriodicalIF":2.9,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10854482/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139707510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Species of Annulotrema (Monopisthocotylea, Dactylogyridae) parasitising African tetras (Characiformes, Alestidae) in the Phongolo River, South Africa with the description of four new species. 南非 Phongolo 河中寄生于非洲哲罗鱼（Characiformes, Alestidae）的 Annulotrema（Monopisthocotylea, Dactylogyridae）物种，并描述了四个新物种。

IF 2.3 2区医学

Parasite Pub Date : 2024-01-01 Epub Date: 2024-10-31 DOI: 10.1051/parasite/2024066

Maria Lujza Kičinjaová, Iva Přikrylová, Mária Seifertová, Eva Řehulková, Milan Gelnar, Nico J Smit

{"title":"Species of Annulotrema (Monopisthocotylea, Dactylogyridae) parasitising African tetras (Characiformes, Alestidae) in the Phongolo River, South Africa with the description of four new species.","authors":"Maria Lujza Kičinjaová, Iva Přikrylová, Mária Seifertová, Eva Řehulková, Milan Gelnar, Nico J Smit","doi":"10.1051/parasite/2024066","DOIUrl":"10.1051/parasite/2024066","url":null,"abstract":"Species of Alestidae are known to be parasitised by dactylogyrid monogeneans representing three genera, Afrocleidodiscus Paperna, 1969, Annulotrema Paperna & Thurston, 1969, and Characidotrema Paperna & Thurston, 1968. The objective of the present study was to investigate the species diversity of Monopisthocotylea of African tetras from the Lower Phongolo River and floodplain in South Africa. Four new and two previously described species of Annulotrema were identified from the gills of three species of African tetras, Brycinus imberi, Hydrocynus vittatus, and Micralestes acutidens. The collected parasites were studied using two complementary approaches: morphology of hard sclerotised structures, and molecular markers using rDNA sequence data (28S rDNA, 18S rDNA, and ITS1). Three new species, Annulotrema arcum n. sp., Annulotrema caputfemoris n. sp., and Annulotrema strepsiceros n. sp., were described from B. imberi and one species, Annulotrema retortum n. sp., from M. acuditens. Two previously described species, Annulotrema pikoides Guégan, Lambert and Birgi, 1988 and A. pseudonili Kičinjaová and Řehulková, 2017, were newly recorded from H. vittatus in South Africa. Annulotrema arcum n. sp. and A. caputfemoris n. sp. share similar male copulatory organ morphologies, suggesting a close phylogenetic relationship as sister taxa. Despite weak nodal support, A. strepsiceros n. sp. shows morphological congruence with the former two species, reinforcing their molecular linkage. The present study shows a critical need for the exploration of monopisthocotylean diversity and the paucity of available molecular data of representatives from this group.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"31 ","pages":"67"},"PeriodicalIF":2.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11527425/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142558431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Untangling the Derogenes varicus species complex in Scandinavian waters and the Arctic: description of Derogenes abba n. sp. (Trematoda, Derogenidae) from Hippoglossoides platessoides and new host records for D. varicus (Müller, 1784) sensu stricto. 在斯堪的纳维亚水域和北极地区解开 Derogenes varicus 的物种复合体：描述来自 Hippoglossoides platessoides 的 Derogenes abba n. sp.（吸虫纲，Derogenidae）和 D. varicus (Müller, 1784) sensu stricto 的新宿主记录。

IF 2.9 2区医学

Parasite Pub Date : 2024-01-01 Epub Date: 2024-05-22 DOI: 10.1051/parasite/2024024

Chahinez Bouguerche, Daniel C Huston, Egil Karlsbakk, Mohammed Ahmed, Oleksandr Holovachov

{"title":"Untangling the Derogenes varicus species complex in Scandinavian waters and the Arctic: description of Derogenes abba n. sp. (Trematoda, Derogenidae) from Hippoglossoides platessoides and new host records for D. varicus (Müller, 1784) sensu stricto.","authors":"Chahinez Bouguerche, Daniel C Huston, Egil Karlsbakk, Mohammed Ahmed, Oleksandr Holovachov","doi":"10.1051/parasite/2024024","DOIUrl":"10.1051/parasite/2024024","url":null,"abstract":"Several studies have shown that the euryxenic trematode Derogenes varicus (Müller, 1784) represents a species complex. Four lineages have been designated (DV1-4) with the DV1 clade corresponding to D. varicus sensu stricto. Herein, we investigate newly collected specimens of D. varicus sensu lato from Scandinavian and Arctic waters using integrative taxonomy. The trematodes were collected from Melanogrammus aeglefinus, Eutrigla gurnardus, Trachinus draco, and Merluccius merluccius off the Atlantic coast of Sweden and from Hippoglossoides platessoides from Arctic Svalbard. 28S sequences of derogenids from Sweden were identical to D. varicus sensu stricto, confirming its euryxeny. The 28S sequences of Derogenes sp. from H. platessoides were identical to Derogenes DV2 and differed from D. varicus sensu stricto by 3% and from Derogenes DV3 by 2%. The 28S sequence divergences of Derogenes sp. from H. platessoides with D. ruber and D. lacustris were 3 and 10%, respectively. ITS2 and cox1 divergences between Derogenes sp. from H. platessoides and other Derogenes species/lineages were at levels of interspecific differences. The species from H. platessoides is described here as D. abba n. sp. We also examined the type material of Progonus muelleri (Levinsen, 1881), the type and only species of the genus Progonus, with redescription and designations of paralectotypes. Based on specimens from Theodor Odhner's collections at the Swedish Museum of Natural History, SMNH, Stockholm, we provide novel morphological and anatomical data for D. varicus sensu lato species complex. Lastly, we investigated Arthur Looss's \"lost collection\" of Trematodes at the SMNH and characterised a putative species Derogenes sp. \"limula\".","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"31 ","pages":"26"},"PeriodicalIF":2.9,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11110757/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141076604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular characterization of EcCLP1, a new putative cathepsin L protease from Echinococcus canadensis. EcCLP1 的分子特征，ECLP1 是一种来自犬棘球蚴的新的推定 cathepsin L 蛋白酶。

IF 2.3 2区医学

Parasite Pub Date : 2024-01-01 Epub Date: 2024-07-09 DOI: 10.1051/parasite/2024036

Ariel Naidich, Ariana M Gutierrez, Federico Camicia

{"title":"Molecular characterization of EcCLP1, a new putative cathepsin L protease from Echinococcus canadensis.","authors":"Ariel Naidich, Ariana M Gutierrez, Federico Camicia","doi":"10.1051/parasite/2024036","DOIUrl":"10.1051/parasite/2024036","url":null,"abstract":"Echinococcus granulosus sensu lato is a platyhelminth parasite and the etiological cause of cystic echinococcosis (CE), a zoonotic and neglected disease that infects animals and humans worldwide. As a part of the biological arsenal of the parasite, cathepsin L proteases are a group of proteins that are believed to be essential for parasite penetration, immune evasion, and establishment in the tissues of the host. In this work, we have cloned and sequenced a new putative cathepsin L protease from Echinococcus canadensis (EcCLP1). The bioinformatic analysis suggests that EcCLP1 could be synthesized as a zymogen and activated after proteolytic cleavage. The multiple sequence alignment with other cathepsin proteases reveals important functional conserved features like a conserved active site, an N-linked glycosylation residue, a catalytic triad, an oxyanion hole, and three putative disulfide bonds. The phylogenetic analysis suggests that EcCLP1 could indeed be a cathepsin L cysteine protease from clade 1 as it grouped with cathepsins from other species in this clade. Modeling studies suggest that EcCLP1 has two domains forming a cleft where the active site is located and an occluding role for the propeptide. The transcriptomic analysis reveals different levels of cathepsin transcript expression along the different stages of the parasite life cycle. The whole-mount immunohistochemistry shows an interesting superficial punctate pattern of staining which suggests a secretory pattern of expression. The putative cathepsin L protease characterized here may represent an interesting tool for diagnostic purposes, vaccine design, or a new pharmacological target for antiparasitic intervention.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"31 ","pages":"39"},"PeriodicalIF":2.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11242924/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141590987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prevalence and risk factors of Toxoplasma gondii infection in dairy cattle from the Western Region of Thailand. 泰国西部地区奶牛弓形虫感染的流行率和风险因素。

IF 2.3 2区医学

Parasite Pub Date : 2024-01-01 Epub Date: 2024-07-09 DOI: 10.1051/parasite/2024038

Napasaporn Wannapong, Preeda Lertwatcharasarakul, Theera Rukkwamsuk

{"title":"Prevalence and risk factors of Toxoplasma gondii infection in dairy cattle from the Western Region of Thailand.","authors":"Napasaporn Wannapong, Preeda Lertwatcharasarakul, Theera Rukkwamsuk","doi":"10.1051/parasite/2024038","DOIUrl":"10.1051/parasite/2024038","url":null,"abstract":"In total, 901 dairy cow sera and data were collected from 51 farms in Nakhon Pathom, Ratchaburi and Kanchanaburi provinces (Western Region of Thailand). Serum samples were processed via the multispecies ELISA method to detect IgG antibodies against Toxoplasma gondii infection. The results demonstrated that the calculated true prevalence was 1.48% (95% CI, 0.64-2.75%) for the individual-level and 29.41% (95% CI, 18.71-43%) for the farm-level. The univariate risk factor analysis showed that the number of total owned cats, the presence of stray cats, and the frequency of cleaning per day were significant factors (p < 0.2). These three factors were subjected to logistic regression analysis, and the results revealed that the frequency of cleaning farms per day was a potential risk factor for T. gondii-seropositive farms (OR = 2.745, 95% CI, 1.15-8.69, p = 0.02). The frequency of cleaning might increase the T. gondii oocyst distribution within the barn area, thus increasing the possibility of infection. Our findings show that T. gondii continues to circulate in the dairy cow population in the western part of Thailand. The presence of cats on farms was not found to be associated with T. gondii infection, but the high frequency of cleaning the floor was, and contributed to the potential risk of infection.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"31 ","pages":"38"},"PeriodicalIF":2.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11242923/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141590988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0