Parasite最新文献

Identification of two new genetic loci for high-resolution genotyping of Enterocytozoon bieneusi.

IF 2.3 2区医学

Parasite Pub Date : 2025-01-01 Epub Date: 2025-01-31 DOI: 10.1051/parasite/2025002

Xinan Meng, Yonglin Ou, Wen Jiang, Yaqiong Guo, Lihua Xiao, Yaoyu Feng, Na Li

{"title":"Identification of two new genetic loci for high-resolution genotyping of Enterocytozoon bieneusi.","authors":"Xinan Meng, Yonglin Ou, Wen Jiang, Yaqiong Guo, Lihua Xiao, Yaoyu Feng, Na Li","doi":"10.1051/parasite/2025002","DOIUrl":"10.1051/parasite/2025002","url":null,"abstract":"In addition to the ribosomal internal transcribed spacer (ITS) locus, four loci (MS1, MS3, MS4, and MS7) have been identified to develop multilocus sequence typing tools for high-resolution genotyping of Enterocytozoon bieneusi in previous studies. However, the use of only five loci was insufficient for population genetic analysis of E. bieneusi from diverse hosts. In this study, comparison of a clinical genome sequence (C44566) with the whole genome sequence of an E. bieneusi isolate (H348) in GenBank led to the selection of the hypothetical protein 1 (hp1) and tubulin 1 (tub1) loci. Further analysis of the two loci with 156 E. bieneusi-positive samples showed high sequence polymorphisms in ITS Groups 1-6 and 10. Altogether, 30 and 23 sequence types were identified at hp1 and tub1, respectively. Genotyping based on the two loci confirmed the lack of genetic differentiation between Group 1 and Group 2 genotypes, as previously reported. Moreover, the genotypes in Groups 4 and 5 are more divergent from other genotypes within Groups 1-10. However, isolates in Group 11 and 12 could not be amplified at the hp1 and tub1 loci, supporting the previous conclusion of genetic uniqueness of the two genotype groups. The identified genetic markers and generated data could be used to develop a multilocus sequence typing tool for high-resolution genotyping of E. bieneusi, which would also have implications for understanding the taxonomy of Enterocytozoon spp., the public health significance of E. bieneusi in animals, and sources of E. bieneusi infections in humans.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"32 ","pages":"6"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11784105/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143067122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multifaceted taxonomy of two Dactylogyrus species on Enteromius paludinosus: Integrating light microscopy, scanning electron microscopy and molecular approaches.

IF 2.3 2区医学

Parasite Pub Date : 2025-01-01 Epub Date: 2025-01-30 DOI: 10.1051/parasite/2024077

Mpho Maduenyane, Quinton Marco Dos Santos, Annemariè Avenant-Oldewage

{"title":"Multifaceted taxonomy of two Dactylogyrus species on Enteromius paludinosus: Integrating light microscopy, scanning electron microscopy and molecular approaches.","authors":"Mpho Maduenyane, Quinton Marco Dos Santos, Annemariè Avenant-Oldewage","doi":"10.1051/parasite/2024077","DOIUrl":"10.1051/parasite/2024077","url":null,"abstract":"Dactylogyrus Diesing, 1850 is the most speciose genus of platyhelminths with more than 900 species, and over a hundred species recorded from Africa. Of the latter, six are from the straightfin barb, Enteromius paludinosus (Peters). Dactylogyrus teresae Mashego, 1983 and Dactylogyrus dominici Mashego, 1983 were collected from E. paludinosus in the Vaal River system, Gauteng, South Africa and their taxonomic data revised using standard protocols and modern approaches, alongside the type material. Whole worms were mounted on glass slides with glycerine ammonium picrate (GAP) and studied using light microscopy (LM). For scanning electron microscopy (SEM), whole worms were placed on concavity slides and the soft tissue digested to release the sclerotised copulatory organs and haptoral sclerites. A combination of these approaches (LM and SEM) was employed for the first time to study the sclerotised structures of GAP-mounted material. Soft tissues of SEM analysed specimens were genetically characterised using CO1 mtDNA, 18S-ITS1-5.8S rDNA and partial 28S rDNA fragments. Phylogenetic topologies were constructed using Bayesian inference. Results confirmed the morphologic and genetic distinctness of D. dominici and D. teresae, highlighting the importance of studying the varying orientations of specifically the vagina and transverse bar. This study presents a new locality record, the first SEM study of isolated sclerotised structures, as well as the first molecular data for the Dactylogyrus afrobarbae-like species. The multifaceted approaches applied to the same specimen in this study enabled improved resolution of individual specimens, showing promise for studies where limited specimens are available.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"32 ","pages":"5"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11784097/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143067125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expansion of the known host range of Giardia duodenalis and Cryptosporidium spp. in captive wildlife at Beijing Zoo.

IF 2.3 2区医学

Parasite Pub Date : 2025-01-01 Epub Date: 2025-01-23 DOI: 10.1051/parasite/2024079

Qianming Zhao, Zhiyang Pei, Yongqiang He, Ting Jia, Yanzhen Zhang, Mengjun Zheng, Zhenjie Zhang, Meng Qi

{"title":"Expansion of the known host range of Giardia duodenalis and Cryptosporidium spp. in captive wildlife at Beijing Zoo.","authors":"Qianming Zhao, Zhiyang Pei, Yongqiang He, Ting Jia, Yanzhen Zhang, Mengjun Zheng, Zhenjie Zhang, Meng Qi","doi":"10.1051/parasite/2024079","DOIUrl":"10.1051/parasite/2024079","url":null,"abstract":"Giardia duodenalis and Cryptosporidium spp. are significant zoonotic parasites that cause diarrhea and affect a diverse range of hosts. This study aimed to investigate the infection rates of these parasites in captive wildlife at Beijing Zoo. A total of 445 fecal samples were collected and analyzed using PCR. The infection rate of G. duodenalis was 3.82% (17/445), with assemblage A (n = 1), assemblage B (n = 13), and assemblage E (n = 3) identified. The infection rate of Cryptosporidium spp. was 0.22% (1/445), with only one instance of C. ryanae identified from cattle (Bos taurus). To the best of our knowledge, this study enhances the understanding of the host range of G. duodenalis in captive wildlife within China, highlighting infections in Corsac Fox (Vulpes corsac), Indian Rhinoceros (Rhinoceros unicornis), and Giraffe (Giraffa camelopardalis). These findings indicate that the infection rates of G. duodenalis and Cryptosporidium spp. in captive wildlife at Beijing Zoo are low, while showing that the known host range of G. duodenalis is expanding.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"32 ","pages":"4"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11756238/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143024300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of a new fish trypanosome from the large yellow croaker (Larimichthys crocea) and description of its impact on host pathology, blood biochemical parameters and immune responses. 大黄鱼一种新锥虫的鉴定及其对宿主病理、血液生化指标和免疫反应的影响。

IF 2.3 2区医学

Parasite Pub Date : 2025-01-01 Epub Date: 2025-01-22 DOI: 10.1051/parasite/2024078

Xiaoao Yang, Pengzhi Qi, Zhen Tao, Qingwei Zhang, Yanjie Wang, Denghui Zhu, Xiaojun Yan, Peipei Fu, Baoying Guo

{"title":"Identification of a new fish trypanosome from the large yellow croaker (Larimichthys crocea) and description of its impact on host pathology, blood biochemical parameters and immune responses.","authors":"Xiaoao Yang, Pengzhi Qi, Zhen Tao, Qingwei Zhang, Yanjie Wang, Denghui Zhu, Xiaojun Yan, Peipei Fu, Baoying Guo","doi":"10.1051/parasite/2024078","DOIUrl":"10.1051/parasite/2024078","url":null,"abstract":"The aim of this study was to clarify the taxonomic identification of a hemoflagellate and assess the effect of trypanosome infection on Larimichthys crocea. Giemsa staining showed the presence of three morphotypes of trypomastigotes. The trypanosomes had the following morphological characteristics: a slender body with a long flagellum at the front; body size 12.30-30.90 × 1.13-2.33 μm; elongated oval nucleus situated in the median region; kinetoplast small, oval, located at the posterior end. The parasite had significant morphological differences from Trypanosoma epinepheli Su, Feng, Jiang, Guo, Liu & Xu, 2014 and Trypanosoma carassii (Mitrofanov) Doflein, 1901. The 18S rDNA sequences of the trypanosome from L. crocea had the highest homology (98.4%) with T. carassii. Phylogenetic analysis indicated that the parasite clustered with freshwater fish trypanosomes. Based on the differences in morphological characteristics and molecular data, it is considered a new species, Trypanosoma larimichthysi n. sp. Trypanosome infection had no effect on the growth of L. crocea, but significantly increased the concentration of blood urea nitrogen (BUN), and induced pathological changes in the gills, liver, spleen and kidney. The pro-inflammatory immune genes, including TNF-α, IFN-γ, IL-1β, CXCL8 and iNOS, were significantly upregulated in the L. crocea infected with trypanosomes. These results suggest that the trypanosome has negative impacts on host health.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"32 ","pages":"1"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11752739/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143009491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular pathogenesis of Cryptosporidium and advancements in therapeutic interventions. 隐孢子虫的分子致病机理和治疗干预措施的进展。

IF 2.3 2区医学

Parasite Pub Date : 2025-01-01 Epub Date: 2025-02-04 DOI: 10.1051/parasite/2025001

Yilong Lu, Xiaoning Zhang, Zhiyu Guan, Rui Ji, Fujun Peng, Chunzhen Zhao, Wei Gao, Feng Gao

引用次数: 0

Molecular detection of parasites and host preference in wild-caught Culicoides biting midges (Diptera: Ceratopogonidae) in Chiang Mai and Nakhon Si Thammarat Provinces, Thailand. 泰国清迈和那空府野生库蠓（双翅目：蠓科）寄生虫分子检测及寄主偏好

IF 2.3 2区医学

Parasite Pub Date : 2025-01-01 Epub Date: 2025-01-22 DOI: 10.1051/parasite/2024082

Baby Kyi Soe, Saowalak Kaewmee, Chonlada Mano, Urassaya Pattanawong, Nopporn Tipparawong, Padet Siriyasatien, Derek Gatherer, Michael D Urbaniak, Paul A Bates, Narissara Jariyapan

{"title":"Molecular detection of parasites and host preference in wild-caught Culicoides biting midges (Diptera: Ceratopogonidae) in Chiang Mai and Nakhon Si Thammarat Provinces, Thailand.","authors":"Baby Kyi Soe, Saowalak Kaewmee, Chonlada Mano, Urassaya Pattanawong, Nopporn Tipparawong, Padet Siriyasatien, Derek Gatherer, Michael D Urbaniak, Paul A Bates, Narissara Jariyapan","doi":"10.1051/parasite/2024082","DOIUrl":"10.1051/parasite/2024082","url":null,"abstract":"Culicoides biting midges (Diptera: Ceratopogonidae) have been reported as potential vectors for haemoparasites. Information about host-vector-parasite specificity is required to confirm their status. Here, molecular detection of haemosporidians, Leishmania, trypanosomatids, and filarial nematodes in biting midges was conducted to understand their potential role as vectors, and their host preference was determined. Wild-caught biting midges were collected from six different localities of Chiang Mai and Nakhon Si Thammarat provinces, Thailand. A total of 6,578 individual Culicoides (170 males, 6,408 females) comprising 15 species of six sub-genera and two groups were collected. Also, 738 parous females and 29 engorged females were examined for parasites and host blood meals, respectively. Culicoides arakawae, C. mahasarakhamense, C. peregrinus and C. innoxius in Chiang Mai province, and C. innoxius and C. peregrinus in Nakhon Si Thammarat province were the most dominant species. Leucocytozoon spp., Leucocytozoon caulleryi and Plasmodium juxtanucleare were identified in five Culicoides species including C. mahasarakhamense, C. arakawae, C. oxystoma, C. fulvus, and C. guttifer. This study is the first record of L. caulleryi in the biting midge C. arakawae in Thailand. Blood meal analysis revealed that Culicoides primarily fed on cattle (17/29, 58.6%), followed by chickens (10/29, 34.5%), and humans (2/29, 6.9%). Our findings confirmed the existence of several Culicoides species in Thailand, which might be potential vectors for transmission of haemosporidians (Leucocytozoon and Plasmodium). Information from host blood meal analyses underlined their preference for large mammals, followed by domestic chickens. More anthropophilic Culicoides species remain to be discovered.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"32 ","pages":"2"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11752737/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143009449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Population structure of Clinostomum complanatum (Trematoda: Digenea) with new data on haplotype diversity of flukes from Slovakia and Italy. 斯洛伐克和意大利吸虫单倍型多样性的新数据及吸虫种群结构。

IF 2.3 2区医学

Parasite Pub Date : 2025-01-01 Epub Date: 2025-01-22 DOI: 10.1051/parasite/2024080

Ľudmila Juhásová, Eva Čisovská Bazsalovicsová, Monica Caffara, Alžbeta Radačovská, Andrea Gustinelli, Lucia Dinisová, Yaroslav Syrota, Ivica Králová-Hromadová

{"title":"Population structure of Clinostomum complanatum (Trematoda: Digenea) with new data on haplotype diversity of flukes from Slovakia and Italy.","authors":"Ľudmila Juhásová, Eva Čisovská Bazsalovicsová, Monica Caffara, Alžbeta Radačovská, Andrea Gustinelli, Lucia Dinisová, Yaroslav Syrota, Ivica Králová-Hromadová","doi":"10.1051/parasite/2024080","DOIUrl":"10.1051/parasite/2024080","url":null,"abstract":"The fluke Clinostomum complanatum, a parasite of piscivorous birds, but also reptiles and rarely mammals, has established several foci in the western Palaearctic regions. Previous studies pointed out the complicated taxonomy of the genus, but broader population genetic analysis of C. complanatum has not yet been carried out. The aim of this study was to determine the structure, intraspecific variability, and diversity of mitochondrial cox1 haplotypes of C. complanatum from different localities in Slovakia (Danube floodplain forests) and Italy (Emilia-Romagna and Tuscany), as well as to evaluate the interrelationships among populations from Europe, the Middle East, and North Africa. The genetic structure of C. complanatum from Slovakia and Italy was represented by a great number of haplotypes, showing stable populations with high intraspecific diversity. The haplotypes of samples from other localities (Romania, Turkey, Egypt, and Iran) showed possible gene flow among the populations from Central Europe down to the Mediterranean region, North Africa, and the Middle East. The genetic homogeneity of these samples can be linked to the distribution and migratory routes of the definitive hosts, aquatic piscivorous birds, mainly herons and cormorants, that spread parasite eggs among the continents.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"32 ","pages":"3"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11752738/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143009450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correlation between imaging features and rEm18 antibodies in alveolar echinococcosis: results from a multicenter study in France.

IF 2.3 2区医学

Parasite Pub Date : 2025-01-01 Epub Date: 2025-02-07 DOI: 10.1051/parasite/2024076

Gabriel Simon, Frédéric Grenouillet, Carine Richou, Eric Delabrousse, Oleg Blagoskonov, Anne Minello, Gerard Thiefin, Emilia Frentiu, Martine Wallon, Solange Bresson-Hadni, Paul Calame

{"title":"Correlation between imaging features and rEm18 antibodies in alveolar echinococcosis: results from a multicenter study in France.","authors":"Gabriel Simon, Frédéric Grenouillet, Carine Richou, Eric Delabrousse, Oleg Blagoskonov, Anne Minello, Gerard Thiefin, Emilia Frentiu, Martine Wallon, Solange Bresson-Hadni, Paul Calame","doi":"10.1051/parasite/2024076","DOIUrl":"https://doi.org/10.1051/parasite/2024076","url":null,"abstract":"Background: To correlate imaging features of alveolar echinococcosis (AE) with the antibodies to recombinant Em18 (rEm18AB) at diagnosis and evaluate initial imaging features associated with serologic response, this retrospective study used data from the prospective multicenter EchinoVISTA study (NCT02876146). Imaging and serology were performed at diagnosis and during follow-up. Univariate and multivariate analyses were used to evaluate imaging features associated with the rEm18AB index. Follow-up analyses evaluated the imaging features associated with serologic response (defined as a 50% reduction in the baseline value within 2 years) in non-operated patients treated with albendazole alone.Results: From June 2012 to July 2016, 45 patients were included, with 8/45 (18%) having an rEm18AB index < 1. Maximum lesion size (76 mm [IQR = 57-93] vs. 36 mm [IQR = 26-51], p = 0.006), microcyst percentage (70% [IQR = 3-8] vs. 20% [IQR = 0.5-3.5], p = 0.004), and maximum standardized uptake value (SUV) on fluorodeoxyglucose positron emission tomography (5.1 [IQR = 4.4-6.2] vs. 2.6 [IQR = 2.4-3.9], p = 0.001) were associated with an rEm18AB index > 1. In patients treated with albendazole, serologic responders at 2 years had smaller lesions (5.3 [IQR = 3.8-72] vs. 3.5 [IQR = 2.7-3.7], p = 0.010) with less pedicle involvement, and lower initial rEm18AB index (2.98 ± 1.63 vs. 7.81 ± 3.95, p = 0.011).Conclusion: Maximum lesion size, percentage of microcysts within the lesion, and maximum lesion SUV are significant imaging features of AE correlated with the rEm18AB index. Serologic response at 2 years occurs primarily in patients with small lesions and a low rEm18AB index.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"32 ","pages":"8"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143365559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular characterization of common zoonotic protozoan parasites and bacteria causing diarrhea in dairy calves in Ningxia Hui Autonomous Region, China. 导致中国宁夏回族自治区奶牛犊牛腹泻的常见人畜共患病原生动物寄生虫和细菌的分子特征。

IF 2.3 2区医学

Parasite Pub Date : 2024-01-01 Epub Date: 2024-10-01 DOI: 10.1051/parasite/2024059

Jia-Qi Zhao, Ying-Ying Fan, Yun-Duan Lei, Ding Liu, Jun-Wei Wang, Xin Yang, Jun-Ke Song, Guang-Hui Zhao

{"title":"Molecular characterization of common zoonotic protozoan parasites and bacteria causing diarrhea in dairy calves in Ningxia Hui Autonomous Region, China.","authors":"Jia-Qi Zhao, Ying-Ying Fan, Yun-Duan Lei, Ding Liu, Jun-Wei Wang, Xin Yang, Jun-Ke Song, Guang-Hui Zhao","doi":"10.1051/parasite/2024059","DOIUrl":"10.1051/parasite/2024059","url":null,"abstract":"Diarrhea caused by zoonotic pathogens is one of the most common diseases in dairy calves, threatening the health of young animals. Humans are also at risk, in particular children. To explore the pathogens causing diarrhea in dairy calves, the present study applied PCR-based sequencing tools to investigate the occurrence and molecular characteristics of three parasites (Cryptosporidium spp., Giardia duodenalis, and Enterocytozoon bieneusi) and three bacterial pathogens (Escherichia coli, Clostridium perfringens, and Salmonella spp.) in 343 fecal samples of diarrheic dairy calves from five farms in Lingwu County, Ningxia Hui Autonomous Region, China. The total positive rate of these pathogens in diarrheic dairy calves was 91.0% (312/343; 95% CI, 87.9-94.0), with C. perfringens (61.5%, 211/343; 95% CI, 56.3-66.7) being the dominant one. Co-infection with two to five pathogens was found in 67.3% (231/343; 95% CI, 62.4-72.3) of investigated samples. There were significant differences (p < 0.05) in the positive rates of Cryptosporidium spp. and diarrheagenic E. coli among farms, age groups, and seasons. Two Cryptosporidium species (C. parvum and C. bovis) and five gp60 subtypes of C. parvum (IIdA15G1, IIdA20G1, IIdA19G1, IIdA14G1, and a novel IIdA13G1) were identified. Two assemblages (assemblage E and zoonotic assemblage A) of G. duodenalis and six ITS genotypes of E. bieneusi (J, Henan-IV, EbpC, I, EbpA, and ESH-01) were observed. Four virulence genes (eaeA, stx1, stx2, and st) of diarrheagenic E. coli and one toxin type (type A) of C. perfringens were detected. Our study enriches our knowledge on the characteristics and zoonotic potential of diarrhea-related pathogens in dairy calves.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"31 ","pages":"60"},"PeriodicalIF":2.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11444552/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142366105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel gene arrangement in the mitochondrial genome of Aspersentis megarhynchus (Acanthocephala, Echinorhynchida, Heteracanthocephalidae), and its phylogenetic implications. Aspersentis megarhynchus（棘鱼科、棘鱼属、异棘鱼科）线粒体基因组中的新基因排列及其系统发育意义。

IF 2.3 2区医学

Parasite Pub Date : 2024-01-01 Epub Date: 2024-10-08 DOI: 10.1051/parasite/2024064

Yuan-Yuan Xie, Hui-Xia Chen, Tetiana A Kuzmina, Olga Lisitsyna, Liang Li

{"title":"Novel gene arrangement in the mitochondrial genome of Aspersentis megarhynchus (Acanthocephala, Echinorhynchida, Heteracanthocephalidae), and its phylogenetic implications.","authors":"Yuan-Yuan Xie, Hui-Xia Chen, Tetiana A Kuzmina, Olga Lisitsyna, Liang Li","doi":"10.1051/parasite/2024064","DOIUrl":"10.1051/parasite/2024064","url":null,"abstract":"The Heteracanthocephalidae Petrochenko, 1956 is a rare family of acanthocephalans mainly parasitic in fishes. The pattern of mitogenomic evolution of the Heteracanthocephalidae is still unknown, and the phylogenetic relationships of the Heteracanthocephalidae with the other 14 families within the order Echinorhynchida remain unclear. In the present study, the complete mitochondrial genome of Aspersentis megarhynchus (von Linstow, 1892) Golvan, 1960 was sequenced and annotated for the first time, which represents the first mitogenomic data for the genus Aspersentis and also for the family Heteracanthocephalidae. The mitogenome of A. megarhynchus has 14,661 bp and includes 36 genes, containing 12 protein-coding genes (PCGs) (missing atp8), 22 tRNA genes, and 2 ribosomal RNAs (rrnS and rrnL), plus two non-coding regions. Comparative mitochondrial genomic analysis revealed that the presence of translocations of several tRNA genes (trnV, trnE, and trnT) and the gene arrangement in the mitogenome of A. megarhynchus represents a new type in Acanthocephala. Moreover, the mitogenomic phylogenetic results based on concatenated amino acid sequences of 12 protein-coding genes strongly supported the validity of the Heteracanthocephalidae and suggested close affinity between the Heteracanthocephalidae and Echinorhynchidae in the order Echinorhynchida.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"31 ","pages":"63"},"PeriodicalIF":2.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11460826/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142392265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0