IF 2.9 2区医学

Parasite Pub Date : 2024-05-17 DOI: 10.1051/parasite/2024027

Liu-Shu Jia, Zhan Liu, Shunhai Zhu, Qiping Zhao, Hong-Yu Han, Huan-Zhi Zhao, Yu Yu, Hui Dong

{"title":"Quantitative phosphoproteomic analysis of chicken DF-1 cells infected with Eimeria tenella, using tandem mass tag (TMT) and parallel reaction monitoring (PRM) mass spectrometry","authors":"Liu-Shu Jia, Zhan Liu, Shunhai Zhu, Qiping Zhao, Hong-Yu Han, Huan-Zhi Zhao, Yu Yu, Hui Dong","doi":"10.1051/parasite/2024027","DOIUrl":"https://doi.org/10.1051/parasite/2024027","url":null,"abstract":"Eimeria tenella is an obligate intracellular parasite which causes great harm to the poultry breeding industry. Protein phosphorylation plays a vital role in host cell–E. tenella interactions. However, no comprehensive phosphoproteomic analyses of host cells at various phases of E. tenella infection have been published. In this study, quantitative phosphoproteomic analysis of chicken embryo DF-1 fibroblasts that were uninfected (UI) or infected with E. tenella for 6 h (PI6, the early invasion phase) or 36 h (PI36, the trophozoite development phase) was conducted. A total of 10,122 phosphopeptides matched to 3,398 host cell phosphoproteins were identified and 13,437 phosphorylation sites were identified. Of these, 491, 1,253, and 275 differentially expressed phosphorylated proteins were identiﬁed in the PI6/UI, PI36/UI, and PI36/PI6 comparisons, respectively. KEGG pathway enrichment analysis showed that E. tenella modulated host cell processes through phosphorylation, including focal adhesion, regulation of the actin cytoskeleton, and FoxO signaling to support its early invasion phase, and modulating adherens junctions and the ErbB signaling pathway to favor its trophozoite development. These results enrich the data on the interaction between E. tenella and host cells and facilitate a better understanding of the molecular mechanisms underlying host–parasite relationships.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140963555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Geoepidemiology, seroprevalence and factors associated with Toxoplasma gondii infection in domicilied cats from Paraíba (Brazil) 巴西帕拉伊巴州家猫的地理分布、血清流行率以及与弓形虫感染相关的因素

IF 2.9 2区医学

Parasite Pub Date : 2024-05-17 DOI: 10.1051/parasite/2024017

Ana Letícia Pereira Fernandes, Mariana de Melo Alves, Jordania Oliveira Silva, Ividy Bison, Ariana de Castro Tavares Silva, R. Parentoni, José Rômulo Soares Dos Santos, T. Feitosa, V. Vilela, Arthur Willian de Lima Brasil

{"title":"Geoepidemiology, seroprevalence and factors associated with Toxoplasma gondii infection in domicilied cats from Paraíba (Brazil)","authors":"Ana Letícia Pereira Fernandes, Mariana de Melo Alves, Jordania Oliveira Silva, Ividy Bison, Ariana de Castro Tavares Silva, R. Parentoni, José Rômulo Soares Dos Santos, T. Feitosa, V. Vilela, Arthur Willian de Lima Brasil","doi":"10.1051/parasite/2024017","DOIUrl":"https://doi.org/10.1051/parasite/2024017","url":null,"abstract":"Toxoplasma gondii is a parasite responsible for toxoplasmosis, an emerging and often neglected zoonosis in South America, particularly Brazil. Felines, the only definitive hosts, excrete oocysts in their feces, potentially infecting all homeotherms. Domestic cats are primarily responsible for contaminating human environments with these oocysts. Monitoring their populations is therefore essential to ensure proper toxoplasmosis prophylaxis. The aim of this study was to estimate the prevalence of T. gondii and exposure factors in a population of owner cats in the city of João Pessoa, Paraíba, Brazil. A total of 267 blood samples were collected from domestic cats aged between 1 and 15 years and tested with an immunofluorescence antibody test. The seroprevalence of antibodies against T. gondii was only 17.22% (46/267 individuals). This result therefore suggests a low contribution of domestic cats to T. gondii contamination of the urban environment. The cats’ age and living environment were identified as risk factors for cat exposure to T. gondii.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140963808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hepatozoon spp. in stray cats from the metropolitan area of Rio de Janeiro, Brazil 巴西里约热内卢大都会地区流浪猫中的肝吸虫属

IF 2.9 2区医学

Parasite Pub Date : 2024-05-17 DOI: 10.1051/parasite/2024026

D. Traversa, A. di Cesare, S. Morelli, B. Paoletti, Marika Grillini, A. F. di Regalbono, Aline da Silva de Mattos Queiroz, F. Beugnet, L. Brustenga, P. Milillo, Luciano Antunes Barros

{"title":"Hepatozoon spp. in stray cats from the metropolitan area of Rio de Janeiro, Brazil","authors":"D. Traversa, A. di Cesare, S. Morelli, B. Paoletti, Marika Grillini, A. F. di Regalbono, Aline da Silva de Mattos Queiroz, F. Beugnet, L. Brustenga, P. Milillo, Luciano Antunes Barros","doi":"10.1051/parasite/2024026","DOIUrl":"https://doi.org/10.1051/parasite/2024026","url":null,"abstract":"In the last few years, the number of studies on feline hepatozoonosis has increased, but our knowledge on the actual species of Hepatozoon and/or different genotypes affecting felines is still incipient. At least three species, namely Hepatozoon felis, H. canis, and H. silvestris, have been isolated from domestic cats in various countries. Additionally, there are indications that other species and genotypes may affect felines in given geographic areas. This study was carried out to investigate the occurrence of Hepatozoon spp. in cats from Niterói, a municipality within the metropolitan area of Rio de Janeiro, Brazil. Individual blood samples were collected from 28 cats enrolled in a spaying/castration program. DNA was extracted from all samples and subjected to sequencing specific for Hepatozoon spp. DNA of H. felis was found in 21/28 cats (75%), and four genetic polymorphisms never described thus far were detected. This is the first report of H. felis in cats living in the State of Rio de Janeiro, and the present data confirm that H. felis is a species complex encompassing different genotypes circulating within cat populations. Further studies are warranted to investigate whether different genotypes have different biology or pathogenicity for felids.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140965750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prevalence of tick-borne pathogens in ticks collected from the wild mountain ungulates mouflon and chamois in 4 regions of France 从法国 4 个地区的野生山地羚羊和驼鹿身上采集的蜱虫中蜱传病原体的流行情况

IF 2.9 2区医学

Parasite Pub Date : 2024-04-10 DOI: 10.1051/parasite/2024011

Jad Habib, Lionel Zenner, Mathieu Garel, Antoine Mercier, Marie-Thérèse Poirel, C. Itty, Joël Appolinaire, Thibaut Amblard, Pierre Benedetti, Frédéric Sanchis, Slimania Benabed, Georges Abi Rizk, Philippe Gibert, G. Bourgoin

{"title":"Prevalence of tick-borne pathogens in ticks collected from the wild mountain ungulates mouflon and chamois in 4 regions of France","authors":"Jad Habib, Lionel Zenner, Mathieu Garel, Antoine Mercier, Marie-Thérèse Poirel, C. Itty, Joël Appolinaire, Thibaut Amblard, Pierre Benedetti, Frédéric Sanchis, Slimania Benabed, Georges Abi Rizk, Philippe Gibert, G. Bourgoin","doi":"10.1051/parasite/2024011","DOIUrl":"https://doi.org/10.1051/parasite/2024011","url":null,"abstract":"Ticks are major vectors of various pathogens of health importance, such as bacteria, viruses and parasites. The problems associated with ticks and vector-borne pathogens are increasing in mountain areas, particularly in connection with global climate change. We collected ticks (n = 2,081) from chamois and mouflon in 4 mountainous areas of France. We identified 6 tick species: Ixodes ricinus, Rhipicephalus bursa, Rh. sanguineus s.l., Haemaphysalis sulcata, H. punctata and Dermacentor marginatus. We observed a strong variation in tick species composition among the study sites, linked in particular to the climate of the sites. We then analysed 791 ticks for DNA of vector-borne pathogens: Babesia/Theileria spp., Borrelia burgdorferi s.l., Anaplasma phagocytophilum, A. marginale, A. ovis, and Rickettsia of the spotted fever group (SFG). Theileria ovis was detected only in Corsica in Rh. bursa. Babesia venatorum (2 sites), Borrelia burgdorferi s.l. (B. afzelii and B. garinii; 2 sites) and Anaplasma phagocytophilum (3 sites) were detected in I. ricinus. Anaplasma ovis was detected at one site in I. ricinus and Rh. sanguineus s.l. SFG Rickettsia were detected at all the study sites: R. monacensis and R. helvetica in I. ricinus at the 3 sites where this tick is present; R. massiliae in Rh. sanguineus s.l. (1 site); and R. hoogstraalii and Candidatus R. barbariae in Rh. bursa in Corsica. These results show that there is a risk of tick-borne diseases for humans and domestic and wild animals frequenting these mountain areas.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140719649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Parasites (Monogenea) of tilapias Oreochromis niloticus and Coptodon rendalli (Cichlidae) in a river spring in Brazil 巴西一条河泉中罗非鱼（Oreochromis niloticus）和罗非鱼（Coptodon rendalli）（Cichlidae）的寄生虫（Monogenea）。

IF 2.9 2区医学

Parasite Pub Date : 2024-04-10 DOI: 10.1051/parasite/2024021

M. Ebert, R. B. Narciso, Diego Henrique Miranda Vieira Dias, M. M. Osaki-Pereira, Maurício Jorge, G. P. de León, R. D. da Silva

{"title":"Parasites (Monogenea) of tilapias Oreochromis niloticus and Coptodon rendalli (Cichlidae) in a river spring in Brazil","authors":"M. Ebert, R. B. Narciso, Diego Henrique Miranda Vieira Dias, M. M. Osaki-Pereira, Maurício Jorge, G. P. de León, R. D. da Silva","doi":"10.1051/parasite/2024021","DOIUrl":"https://doi.org/10.1051/parasite/2024021","url":null,"abstract":"In the present study, we examined 30 individuals of introduced African cichlids, Oreochromis niloticus and Coptodon rendalli, collected in a river spring of the Pardo River, Paranapanema River basin, southeastern Brazil. Based on morphological and molecular analyses of the partial LSU rDNA gene, we identified four species of monogeneans, Cichlidogyrus tilapiae, C. thurstonae, C. mbirizei, and Scutogyrus longicornis on the gills of O. niloticus, whereas individuals of C. rendalli were infested only with C. papernastrema. This is the first record of C. mbirizei and C. papernastrema in tilapias from Brazil. The ecological consequences of the introduction of exotic species of tilapia such as O. niloticus and C. rendalli along with their monogenean parasites in a wild environment represented by a river spring are discussed. Our new molecular data on Cichlidogyrus and Scutogyrus contribute to the investigation of the phylogenetic interrelationships of these widely distributed genera of monogeneans since their species composition is still unsettled.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140717169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cross-species transmission of Cryptosporidium in wild rodents from the southern region of Zhejiang Province of China and its possible impact on public health. 中国浙江省南部地区野生啮齿动物隐孢子虫的跨物种传播及其对公共卫生的可能影响。

IF 2.3 2区医学

Parasite Pub Date : 2024-01-01 Epub Date: 2024-07-01 DOI: 10.1051/parasite/2024033

Yanyan Jiang, Aiying Jiang, Guangxu Ren, Long Wang, Xianming Xin, Zhongying Yuan, Jiani Liu, Zhen Li, Yanbin Sun, Shanshan Zhou, Gang Lu, Huicong Huang, Wei Zhao

{"title":"Cross-species transmission of Cryptosporidium in wild rodents from the southern region of Zhejiang Province of China and its possible impact on public health.","authors":"Yanyan Jiang, Aiying Jiang, Guangxu Ren, Long Wang, Xianming Xin, Zhongying Yuan, Jiani Liu, Zhen Li, Yanbin Sun, Shanshan Zhou, Gang Lu, Huicong Huang, Wei Zhao","doi":"10.1051/parasite/2024033","DOIUrl":"10.1051/parasite/2024033","url":null,"abstract":"Wild rodents serve as reservoirs for Cryptosporidium and are overpopulated globally. However, genetic data regarding Cryptosporidium in these animals from China are limited. Here, we have determined the prevalence and genetic characteristics of Cryptosporidium among 370 wild rodents captured from three distinct locations in the southern region of Zhejiang Province, China. Fresh feces were collected from the rectum of each rodent, and DNA was extracted from them. The rodent species was identified by PCR amplifying the vertebrate cytochrome b gene. Cryptosporidium was detected by PCR amplification and amplicon sequencing the small subunit of ribosomal RNA gene. Positive samples of C. viatorum and C. parvum were further subtyped by analyzing the 60-kDa glycoprotein gene. A positive Cryptosporidium result was found in 7% (26/370) of samples, involving five rodent species: Apodemus agrarius (36), Niviventer niviventer (75), Rattus losea (18), R. norvegicus (155), and R. tanezumi (86). Their respective Cryptosporidium positive rates were 8.3%, 5.3%, 11.1%, 7.1%, and 7.0%. Sequence analysis confirmed the presence of three Cryptosporidium species: C. parvum (4), C. viatorum (1), and C. muris (1), and two genotypes: Cryptosporidium rat genotype IV (16) and C. mortiferum-like (4). Additionally, two subtypes of C. parvum (IIdA15G1 and IIpA19) and one subtype of C. viatorum (XVdA3) were detected. These results demonstrate that various wild rodent species in Zhejiang were concurrently infected with rodent-adapted and zoonotic species/genotypes of Cryptosporidium, indicating that these rodents can play a role in maintaining and dispersing this parasite into the environment and other hosts, including humans.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11216159/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141469992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cryptosporidium spp. in captive snakes from 26 provinces in China: Prevalence, molecular characterization, and symptoms. 中国 26 个省份人工饲养蛇类中的隐孢子虫属：流行率、分子特征和症状。

IF 2.3 2区医学

Parasite Pub Date : 2024-01-01 Epub Date: 2024-08-07 DOI: 10.1051/parasite/2024047

Yilei Zhang, Zhenxiao Lu, Lingru He, Guodong Xiao, Lijie Tian, Jiawei Zhu, Tian Liu, Qiangxin Ou, Haibo Chen, Yew Hwong, Yangjun Kang, Qianming Xu, Qingxun Zhang, Congshan Yang

{"title":"Cryptosporidium spp. in captive snakes from 26 provinces in China: Prevalence, molecular characterization, and symptoms.","authors":"Yilei Zhang, Zhenxiao Lu, Lingru He, Guodong Xiao, Lijie Tian, Jiawei Zhu, Tian Liu, Qiangxin Ou, Haibo Chen, Yew Hwong, Yangjun Kang, Qianming Xu, Qingxun Zhang, Congshan Yang","doi":"10.1051/parasite/2024047","DOIUrl":"10.1051/parasite/2024047","url":null,"abstract":"Snakes are sometimes regarded as pets and are used in traditional Chinese medicine. Cryptosporidium spp. are frequently identified in snakes, representing an important pathogen and causing gastrointestinal diseases. Current data indicate that risk factors for infection and patterns of clinical symptom presentation may differ among Cryptosporidium spp. To better understand the infection status by Cryptosporidium spp., fecal samples were collected from 603 asymptomatic and 147 symptomatic snakes in 26 provinces of China. These samples came from Elaphe guttata, Elaphe obsoleta, Pituophis melanoleucus, Thamnophis sirtalis, Lampropeltis getulus, and Heterodon nasicus. The partial small subunit (SSU) rRNA gene was amplified using nested polymerase chain reaction (PCR) to investigate the infection rate of Cryptosporidium spp., and to assess evolutionary relationships and genetic characterization. A prevalence of 20% was recorded in asymptomatic snakes, with age identified as a significant risk factor. In contrast, 70% of symptomatic snakes were positive for Cryptosporidium spp., with Cryptosporidium serpentis and Cryptosporidium varanii (syn. C. saurophilum). Further analysis revealed a potential association between C. serpentis and regurgitation, and C. varanii and diarrhea, while neither species was linked to flatulence. To our knowledge, this is the first study to report Cryptosporidium spp. and associated clinical signs in symptomatic snakes in China. This study aims to enhance the understanding of Cryptosporidium infections, risk factors, and clinical manifestations in snakes, providing data crucial for the control and prevention of cryptosporidiosis.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11305116/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141897957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantitative phosphoproteomic analysis of chicken DF-1 cells infected with Eimeria tenella, using tandem mass tag (TMT) and parallel reaction monitoring (PRM) mass spectrometry. 利用串联质量标记（TMT）和平行反应监测（PRM）质谱对感染了天牛埃默氏菌的鸡 DF-1 细胞进行定量磷蛋白组学分析。

IF 2.9 2区医学

Parasite Pub Date : 2024-01-01 Epub Date: 2024-05-17 DOI: 10.1051/parasite/2024027

Liu-Shu Jia, Zhan Liu, Shun-Hai Zhu, Qi-Ping Zhao, Hong-Yu Han, Huan-Zhi Zhao, Yu Yu, Hui Dong

{"title":"Quantitative phosphoproteomic analysis of chicken DF-1 cells infected with Eimeria tenella, using tandem mass tag (TMT) and parallel reaction monitoring (PRM) mass spectrometry.","authors":"Liu-Shu Jia, Zhan Liu, Shun-Hai Zhu, Qi-Ping Zhao, Hong-Yu Han, Huan-Zhi Zhao, Yu Yu, Hui Dong","doi":"10.1051/parasite/2024027","DOIUrl":"https://doi.org/10.1051/parasite/2024027","url":null,"abstract":"Eimeria tenella is an obligate intracellular parasite which causes great harm to the poultry breeding industry. Protein phosphorylation plays a vital role in host cell-E. tenella interactions. However, no comprehensive phosphoproteomic analyses of host cells at various phases of E. tenella infection have been published. In this study, quantitative phosphoproteomic analysis of chicken embryo DF-1 fibroblasts that were uninfected (UI) or infected with E. tenella for 6 h (PI6, the early invasion phase) or 36 h (PI36, the trophozoite development phase) was conducted. A total of 10,122 phosphopeptides matched to 3,398 host cell phosphoproteins were identified and 13,437 phosphorylation sites were identified. Of these, 491, 1,253, and 275 differentially expressed phosphorylated proteins were identiﬁed in the PI6/UI, PI36/UI, and PI36/PI6 comparisons, respectively. KEGG pathway enrichment analysis showed that E. tenella modulated host cell processes through phosphorylation, including focal adhesion, regulation of the actin cytoskeleton, and FoxO signaling to support its early invasion phase, and modulating adherens junctions and the ErbB signaling pathway to favor its trophozoite development. These results enrich the data on the interaction between E. tenella and host cells and facilitate a better understanding of the molecular mechanisms underlying host-parasite relationships.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11101204/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140958703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficacy of a topical formulation containing eprinomectin, esafoxolaner and praziquantel (NexGard^® Combo) in the treatment of natural respiratory capillariosis of cats. 含伊普菌素、埃沙福朗和吡喹酮的外用制剂（NexGard® Combo）治疗猫自然呼吸道毛滴虫病的疗效。

IF 2.9 2区医学

Parasite Pub Date : 2024-01-01 Epub Date: 2024-02-04 DOI: 10.1051/parasite/2024005

Angela Di Cesare, Giulia Morganti, Massimo Vignoli, Mariasole Colombo, Fabrizia Veronesi, Antonello Bufalari, Eric Tielemans, Frederic Beugnet, Donato Traversa

{"title":"Efficacy of a topical formulation containing eprinomectin, esafoxolaner and praziquantel (NexGard® Combo) in the treatment of natural respiratory capillariosis of cats.","authors":"Angela Di Cesare, Giulia Morganti, Massimo Vignoli, Mariasole Colombo, Fabrizia Veronesi, Antonello Bufalari, Eric Tielemans, Frederic Beugnet, Donato Traversa","doi":"10.1051/parasite/2024005","DOIUrl":"10.1051/parasite/2024005","url":null,"abstract":"Feline pulmonary capillariosis is a significant disorder due to its distribution and clinical impact. This study evaluated the safety and efficacy of two administrations 28 days apart of a topical solution containing esafoxolaner, eprinomectin and praziquantel (NexGard® Combo) in treating Eucoleus aerophilus (syn. Capillaria aerophila) infection in naturally infected cats. Cats were allocated to two groups: G1 cats (n = 23) received two treatments at study days (SDs) 0 and 28 (±2) and were evaluated for 6 weeks, and G2 cats (n = 17) served as a negative control for 6 weeks and were then treated twice on SDs 42 (±2) and 70 (±2), allowing for an additional 6-week assessment of efficacy. Each cat was subjected to McMaster coproscopy at SDs -7/0, 28 (±2) and 42 (±2) for both groups, 70 (±2) and 84 (±2) only for G2. Clinical examination and chest radiographic images were performed at SDs 0, 28 (±2) and 42 (±2) for G1 and G2, 70 (±2) and 84 (±2) only for G2. The comparison of EPG (eggs per gram of feces), clinical (CS), and radiographic scores (RS) at each time-point was used as a criterion. The efficacy based on the EPG reduction was 99.5% (G1) and 100% (G2) after two administrations of NexGard® Combo 2 weeks apart. At SD 0, no significant differences for CS and RS were recorded between G1 and G2, while a significant reduction (p < 0.05) was observed post-treatment for CS, RS, oculo-nasal discharge, auscultation noises, and cough. Two doses of NexGard® Combo 28 days apart stopped egg shedding and significantly improved clinical alterations in cats infected by E. aerophilus.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10840459/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139692587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Annexin gene family in Spirometra mansoni (Cestoda: Diphyllobothriidae) and its phylogenetic pattern among Platyhelminthes of medical interest. 曼氏螺旋绦虫（绦虫纲：Diphyllobothriidae）的 Annexin 基因家族及其在具有医学意义的扁形动物中的系统发育模式。

IF 2.3 2区医学

Parasite Pub Date : 2024-01-01 Epub Date: 2024-06-21 DOI: 10.1051/parasite/2024034

Xiao Yi Su, Fei Gao, Si Yao Wang, Jing Li, Zhong Quan Wang, Xi Zhang

{"title":"Annexin gene family in Spirometra mansoni (Cestoda: Diphyllobothriidae) and its phylogenetic pattern among Platyhelminthes of medical interest.","authors":"Xiao Yi Su, Fei Gao, Si Yao Wang, Jing Li, Zhong Quan Wang, Xi Zhang","doi":"10.1051/parasite/2024034","DOIUrl":"10.1051/parasite/2024034","url":null,"abstract":"The plerocercoid larvae of Spirometra mansoni are etiological agents of human and animal sparganosis. Annexins are proteins with important roles in parasites. However, our knowledge of annexins in S. mansoni is still inadequate. In this study, 18 new members of the Annexin (ANX) family were characterized in S. mansoni. The clustering analysis demonstrated that all the SmANXs were divided into two main classes, consistent with the patterns of conserved motif organization. The 18 SmANXs were detected at all developmental stages (plerocercoid, adult, and egg) and displayed ubiquitous but highly variable expression patterns in all tissues/organs studied. The representative member rSmANX18 was successfully cloned and expressed. The protein was immunolocalized in the tegument and parenchyma of the plerocercoid and in the tegument, parenchyma, uterus and egg shell of adult worms. The recombinant protein can bind phospholipids with high affinity in a Ca2+-dependent manner, shows high anticoagulant activity and combines with FITC to recognize apoptotic cells. Annexin gene polymorphism and conservative core motif permutation were found in both cestodes and trematodes. SmANXs also revealed high genetic diversity among Platyhelminthes of medical interest. Our findings lay a foundation for further studies on the biological functions of ANXs in S. mansoni as well as other taxa in which ANXs occur.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11195529/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141443171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Parasite最新文献