Comparison of the standard and boosted sterile insect techniques for the suppression of Aedes albopictus populations under semi-field conditions.

Abstract

Innovative control tools are needed against Aedes mosquitoes. The boosted sterile insect technique (bSIT) consists of treating sterile males with a biocide prior to their release to contaminate larval habitats. We compared the efficacy of SIT and boosted SIT to prevent the emergence of adult Aedes albopictus in large cages. We tested two sterile-to-fertile male ratios: 5:1 (SIT5) and 1:1 (SIT1), with and without pyriproxyfen enhancement (bSIT or SIT). The eggs were collected in ovitraps and the immature stages were monitored until adult emergence or up to 15 days after hatching to estimate the relative risk (RR) of adult emergence compared to the control category. The concentration of pyriproxyfen in the ovitrap water did not change when sterile males were released with females or alone (χ² = 0.99, df = 1, p = 0.547). This concentration was higher when the sterile-to-fertile male ratio was increased from 1:1 to 5:1: χ² = 18.8, df = 1, p = 0.006. All four treatment categories were effective in suppressing mosquito populations. With a relative risk RR = 0.194 95% CI [0.128; 0.275], SIT5 was the most effective. Boosted SIT was not as effective as SIT. However, bSIT1 (RR = 0.418 [0.351; 0.492]) and bSIT5 (RR = 0.512 [0.431; 0.596]) were equally effective. Boosted males directly vectored pyriproxyfen to breeding sites. Boosted SIT was more effective than SIT alone with a low sterile-to-fertile male ratio. Under operational conditions, it could be initially deployed to suppress the target population and then switched to standard SIT.