Parasite最新文献_第5页

Bed bug control with various dusts: Efficacy comparison between silicon dioxide, diatomaceous earth, and Sommières earth. 用各种粉尘控制臭虫：二氧化硅、硅藻土和索米埃尔土的功效比较。

IF 2.3 2区医学

Parasite Pub Date : 2024-01-01 Epub Date: 2024-07-24 DOI: 10.1051/parasite/2024040

Halilou Almou Oumarou, Harouna Tahirou Hima, Jean Michel Berenger, Grégory Michel, Olivier Grauby, Philippe Parola, Christelle Pomares, Pascal Delaunay

{"title":"Bed bug control with various dusts: Efficacy comparison between silicon dioxide, diatomaceous earth, and Sommières earth.","authors":"Halilou Almou Oumarou, Harouna Tahirou Hima, Jean Michel Berenger, Grégory Michel, Olivier Grauby, Philippe Parola, Christelle Pomares, Pascal Delaunay","doi":"10.1051/parasite/2024040","DOIUrl":"10.1051/parasite/2024040","url":null,"abstract":"Bed bugs are considered a major public health problem in industrialized countries. Usually, bed bug infestations are managed using a combination of physical and chemical methods. In recent years, new strategies for bed bug control have emerged, particularly the use of dusts like diatomaceous earth and silicon dioxide. However, in Europe, the use of silicon dioxide is restricted to professional, while diatomaceous earth can be harmful to the lungs. This study aimed to assess bed bug mortality rates associated with Sommières earth, green clay, talc, and sodium bicarbonate compared to silicon dioxide and diatomaceous earth from a pest management company, diatomaceous earth for litter conditioner, and diatomaceous earth from a supermarket. We tested permanent exposure, short exposure, horizontal transfer and repellent effect on two bed bug colonies. Sommières earth demonstrated efficacy ranging from 75% to 100% in permanent and short exposures, similar to the efficacy of diatomaceous earth from the pest management company. On the contrary, diatomaceous earth for litter conditioner and diatomaceous earth from a supermarket, green clay, talc, and sodium bicarbonate were found to be ineffective. This study demonstrates, for the first time, the efficacy of Sommières earth against bed bugs, but also highlights the variability in efficacy of diatomaceous earths on bed bugs depending on their quality.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"31 ","pages":"41"},"PeriodicalIF":2.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11271705/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141760254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cryptosporidium spp. prevalence in the general population in Guinea: first large-scale screening study. 几内亚普通人群的隐孢子虫感染率：首次大规模筛查研究。

IF 2.3 2区医学

Parasite Pub Date : 2024-01-01 Epub Date: 2024-11-13 DOI: 10.1051/parasite/2024070

Timothé Guilavogui, Nausicaa Gantois, Jérémy Desramaut, Fode Ibrahima Cissé, Salif Cherif Touré, Bakary Luther Kourouma, Cristian Preda, Magali Chabé, Eric Viscogliosi, Gabriela Certad

{"title":"Cryptosporidium spp. prevalence in the general population in Guinea: first large-scale screening study.","authors":"Timothé Guilavogui, Nausicaa Gantois, Jérémy Desramaut, Fode Ibrahima Cissé, Salif Cherif Touré, Bakary Luther Kourouma, Cristian Preda, Magali Chabé, Eric Viscogliosi, Gabriela Certad","doi":"10.1051/parasite/2024070","DOIUrl":"10.1051/parasite/2024070","url":null,"abstract":"Cryptosporidium is a leading cause of diarrheal mortality in children in Africa and Asia. Despite the public health significance of this parasite, its molecular epidemiology and circulation in Guinea remain poorly understood. Therefore, this study aimed to determine the prevalence and genotype distribution of Cryptosporidium in the Guinean general population. To achieve this, fecal samples were collected from 834 individuals, both with and without digestive disorders, at two hospitals in Conakry. The presence of the parasite in the stool samples was detected using nested PCR targeting the SSU rDNA gene, followed by sequencing of the PCR products for genotyping of the isolates. The PCR-based prevalence was 0.12% for the whole cohort, and 0.2% among adults. The low frequency of Cryptosporidium observed in the current study is thus consistent with the prevalence of this parasite already reported in certain other African countries. The species identified in the positive samples was Cryptosporidium hominis. This study is the first to report the prevalence of Cryptosporidium in the general population of Guinea. Given the potential of this parasite to cause life-threatening diarrhea, further studies are needed to clarify the epidemiology of Cryptosporidium in this country.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"31 ","pages":"70"},"PeriodicalIF":2.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11560126/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142623504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unraveling the pathogenic potential of the Pentatrichomonas hominis PHGD strain: impact on IPEC-J2 cell growth, adhesion, and gene expression. 揭示人型五联单胞菌 PHGD 菌株的致病潜力：对 IPEC-J2 细胞生长、粘附和基因表达的影响。

IF 2.9 2区医学

Parasite Pub Date : 2024-01-01 Epub Date: 2024-03-26 DOI: 10.1051/parasite/2024014

Yibin Zhu, Haiming Cai, Siyun Fang, Hanqin Shen, Zhuanqiang Yan, Dingai Wang, Nanshan Qi, Juan Li, Minna Lv, Xuhui Lin, Junjing Hu, Yongle Song, Xiangjie Chen, Lijun Yin, Jianfei Zhang, Shenquan Liao, Mingfei Sun

{"title":"Unraveling the pathogenic potential of the Pentatrichomonas hominis PHGD strain: impact on IPEC-J2 cell growth, adhesion, and gene expression.","authors":"Yibin Zhu, Haiming Cai, Siyun Fang, Hanqin Shen, Zhuanqiang Yan, Dingai Wang, Nanshan Qi, Juan Li, Minna Lv, Xuhui Lin, Junjing Hu, Yongle Song, Xiangjie Chen, Lijun Yin, Jianfei Zhang, Shenquan Liao, Mingfei Sun","doi":"10.1051/parasite/2024014","DOIUrl":"10.1051/parasite/2024014","url":null,"abstract":"Pentatrichomonas hominis, a flagellated parasitic protozoan, predominantly infects the mammalian digestive tract, often causing symptoms such as abdominal pain and diarrhea. However, studies investigating its pathogenicity are limited, and the mechanisms underlying P. hominis-induced diarrhea remain unclear. Establishing an in vitro cell model for P. hominis infection is imperative. This study investigated the interaction between P. hominis and IPEC-J2 cells and its impact on parasite growth, adhesion, morphology, and cell viability. Co-cultivation of P. hominis with IPEC-J2 cells resulted in exponential growth of the parasite, with peak densities reaching approximately 4.8 × 105 cells/mL and 1.2 × 106 cells/mL at 48 h for initial inoculation concentrations of 104 cells/mL and 105 cells/mL, respectively. The adhesion rate of P. hominis to IPEC-J2 cells reached a maximum of 93.82% and 86.57% at 24 h for initial inoculation concentrations of 104 cells/mL and 105 cells/mL, respectively. Morphological changes in IPEC-J2 cells co-cultivated with P. hominis were observed, manifesting as elongated and irregular shapes. The viability of IPEC-J2 cells exhibited a decreasing trend with increasing P. hominis concentration and co-cultivation time. Additionally, the mRNA expression levels of IL-6, IL-8, and TNF-α were upregulated, whereas those of CAT and CuZn-SOD were downregulated. These findings provide quantitative evidence that P. hominis can promote its growth by adhering to IPEC-J2 cells, inducing morphological changes, reducing cell viability, and triggering inflammatory responses. Further in vivo studies are warranted to confirm these results and enhance our understanding of P. hominis infection.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"31 ","pages":"18"},"PeriodicalIF":2.9,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10964850/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140288716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chemotherapy for the treatment of alveolar echinococcosis: Where are we? 治疗肺泡棘球蚴病的化疗：进展如何？

IF 2.3 2区医学

Parasite Pub Date : 2024-01-01 Epub Date: 2024-09-23 DOI: 10.1051/parasite/2024055

Brice Autier, Florence Robert-Gangneux, Sarah Dion

{"title":"Chemotherapy for the treatment of alveolar echinococcosis: Where are we?","authors":"Brice Autier, Florence Robert-Gangneux, Sarah Dion","doi":"10.1051/parasite/2024055","DOIUrl":"10.1051/parasite/2024055","url":null,"abstract":"Alveolar echinococcosis (AE) is a severe liver disease due to infection with the Echinococcus multilocularis larval stage, called the metacestode. Management of AE is based on benzimidazole chemotherapy (albendazole or mebendazole), associated with surgery when possible. Benzimidazoles are the only compounds recommended for the treatment of AE; however, these are parasitostatic, which means that the parasite can resume growth when treatment is interrupted. Also, benzimidazoles can cause liver dysfunction which may prevent their use. Numerous drugs have been reported to have in vitro activity against E. multilocularis, but few had satisfactory in vivo activity, and none were clearly more effective than benzimidazoles. These drugs belong to various therapeutic categories including anti-infective agents (e.g. amphotericin B, mefloquine, pentamidine derivatives), anti-neoplastic compounds (e.g. imatinib, nilotinib, bortezomib), plant-extracted compounds (e.g. thymol, crocin, carvacrol) and others (e.g. metformin, verapamil, thiaclopride). These treatments are generally of limited interest due to their toxicity, their unfavorable pharmacokinetics, or the scarcity of studies involving humans. Apart from benzimidazoles, only amphotericin B, mefloquine and nitazoxanide have been reported to be used for human AE treatment, with unsatisfactory results. Few studies have aimed at developing innovative strategies for AE drug therapy, such as vectorization of drugs using nanoparticles. Altogether, this review emphasizes the urgent need for new therapeutic strategies in AE management, for which there is currently no curative chemotherapy.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"31 ","pages":"56"},"PeriodicalIF":2.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11418394/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142292913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Limited impact of vector control on the population genetic structure of Glossina fuscipes fuscipes from the sleeping sickness focus of Maro, Chad. 病媒控制对乍得马罗昏睡病重点地区 Glossina fuscipes fuscipes 种群遗传结构的有限影响。

IF 2.9 2区医学

Parasite Pub Date : 2024-01-01 Epub Date: 2024-03-06 DOI: 10.1051/parasite/2024013

Sophie Ravel, Adeline Ségard, Brahim Guihini Mollo, Mahamat Hissène Mahamat, Rafael Argiles-Herrero, Jérémy Bouyer, Jean-Baptiste Rayaisse, Philippe Solano, Mallaye Pèka, Justin Darnas, Adrien Marie Gaston Belem, Wilfrid Yoni, Camille Noûs, Thierry de Meeûs

{"title":"Limited impact of vector control on the population genetic structure of Glossina fuscipes fuscipes from the sleeping sickness focus of Maro, Chad.","authors":"Sophie Ravel, Adeline Ségard, Brahim Guihini Mollo, Mahamat Hissène Mahamat, Rafael Argiles-Herrero, Jérémy Bouyer, Jean-Baptiste Rayaisse, Philippe Solano, Mallaye Pèka, Justin Darnas, Adrien Marie Gaston Belem, Wilfrid Yoni, Camille Noûs, Thierry de Meeûs","doi":"10.1051/parasite/2024013","DOIUrl":"10.1051/parasite/2024013","url":null,"abstract":"Tsetse flies (genus Glossina) transmit deadly trypanosomes to human populations and domestic animals in sub-Saharan Africa. Some foci of Human African Trypanosomiasis due to Trypanosoma brucei gambiense (g-HAT) persist in southern Chad, where a program of tsetse control was implemented against the local vector Glossina fuscipes fuscipes in 2018 in Maro. We analyzed the population genetics of G. f. fuscipes from the Maro focus before control (T0), one year (T1), and 18 months (T2) after the beginning of control efforts. Most flies captured displayed a local genetic profile (local survivors), but a few flies displayed outlier genotypes. Moreover, disturbance of isolation by distance signature (increase of genetic distance with geographic distance) and effective population size estimates, absence of any genetic signature of a bottleneck, and an increase of genetic diversity between T0 and T2 strongly suggest gene flows from various origins, and a limited impact of the vector control efforts on this tsetse population. Continuous control and surveillance of g-HAT transmission is thus recommended in Maro. Particular attention will need to be paid to the border with the Central African Republic, a country where the entomological and epidemiological status of g-HAT is unknown.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"31 ","pages":"13"},"PeriodicalIF":2.9,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10918643/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140050030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

No evidence of pathogenicity of Dientamoeba fragilis following detection in stools: A case-control study. 在粪便中检测到脆弱片阿米巴后，没有证据表明其具有致病性：病例对照研究。

IF 2.3 2区医学

Parasite Pub Date : 2024-01-01 Epub Date: 2024-07-24 DOI: 10.1051/parasite/2024041

Germain Tchamwa Bamini, Eléna Charpentier, Emilie Guemas, Pamela Chauvin, Judith Fillaux, Alexis Valentin, Sophie Cassaing, Sandie Ménard, Antoine Berry, Xavier Iriart

{"title":"No evidence of pathogenicity of Dientamoeba fragilis following detection in stools: A case-control study.","authors":"Germain Tchamwa Bamini, Eléna Charpentier, Emilie Guemas, Pamela Chauvin, Judith Fillaux, Alexis Valentin, Sophie Cassaing, Sandie Ménard, Antoine Berry, Xavier Iriart","doi":"10.1051/parasite/2024041","DOIUrl":"10.1051/parasite/2024041","url":null,"abstract":"Dientamoeba fragilis is a ubiquitous intestinal parasite with detection in the stools that has become increasingly frequent following the advent of PCR as a routine screening tool. However, the pathogenicity of this parasite is still much debated. In order to assess the potentially pathogenic nature of this protozoan, a retrospective case-control study was carried out between January and December 2020 on patients from Toulouse University Hospital, with the aim of evaluating the potential clinical effects and changes in laboratory parameters linked to the presence and load of D. fragilis in stools. After matching age, sex and mode of care (consultation or hospitalisation), no significant difference was observed in the frequency of clinical signs between the 36 patients who tested positive for Dientamoeba fragilis PCR in their stools and the 72 control patients who were PCR negative for this protozoan. The presence of D. fragilis in the faeces was not associated with changes in laboratory parameters. Furthermore, a high digestive load of D. fragilis had no identifiable impact on clinical and laboratory parameters. Only the concomitant presence of Blastocystis sp. in stools was significantly more frequent in the D. fragilis group (uni- and multivariate analysis). Finally, this study showed no significant difference in clinical or laboratory signs between patients carrying Dientamoeba fragilis and the control group, regardless of the intestinal parasite load, suggesting that D. fragilis could be considered a commensal of the digestive tract.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"31 ","pages":"40"},"PeriodicalIF":2.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11271704/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141760280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Four new species of Cichlidogyrus (Platyhelminthes, Monopisthocotyla, Dactylogyridae) from Lake Victoria haplochromine cichlid fishes, with the redescription of C. bifurcatus and C. longipenis. 维多利亚湖单色鳉鱼类中的四个新种，并重新描述了 C. bifurcatus 和 C. longipenis。

IF 2.3 2区医学

Parasite Pub Date : 2024-01-01 Epub Date: 2024-08-07 DOI: 10.1051/parasite/2024039

Tiziana P Gobbin, Maarten P M Vanhove, Ole Seehausen, Martine E Maan, Antoine Pariselle

{"title":"Four new species of Cichlidogyrus (Platyhelminthes, Monopisthocotyla, Dactylogyridae) from Lake Victoria haplochromine cichlid fishes, with the redescription of C. bifurcatus and C. longipenis.","authors":"Tiziana P Gobbin, Maarten P M Vanhove, Ole Seehausen, Martine E Maan, Antoine Pariselle","doi":"10.1051/parasite/2024039","DOIUrl":"10.1051/parasite/2024039","url":null,"abstract":"African cichlids are model systems for evolutionary studies and host-parasite interactions, because of their adaptive radiations and because they harbour many species of monogenean parasites with high host-specificity. Five locations were sampled in southern Lake Victoria: gill-infecting monogeneans were surveyed from 18 cichlid species belonging to this radiation superflock and two others representing two older and distantly related lineages. We found one species of Gyrodactylidae, Gyrodactylus sturmbaueri Vanhove, Snoeks, Volckaert & Huyse, 2011, and seven species of Dactylogyridae. Four are described herein: Cichlidogyrus pseudodossoui n. sp., Cichlidogyrus nyanza n. sp., Cichlidogyrus furu n. sp., and Cichlidogyrus vetusmolendarius n. sp. Another Cichlidogyrus species is reported but not formally described (low number of specimens, morphological similarity with C. furu n. sp.). Two other species are redescribed: C. bifurcatus Paperna, 1960 and C. longipenis Paperna & Thurston, 1969. Our results confirm that the monogenean fauna of Victorian littoral cichlids displays lower species richness and lower host-specificity than that of Lake Tanganyika littoral cichlids. In C. furu n. sp., hooks V are clearly longer than the others, highlighting the need to re-evaluate the current classification system that considers hook pairs III-VII as rather uniform. Some morphological features of C. bifurcatus, C. longipenis, and C. nyanza n. sp. suggest that these are closely related to congeners that infect other haplochromines. Morphological traits indicate that representatives of Cichlidogyrus colonised Lake Victoria haplochromines or their ancestors at least twice, which is in line with the Lake Victoria superflock being colonised by two cichlid tribes (Haplochromini and Oreochromini).","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"31 ","pages":"46"},"PeriodicalIF":2.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11305117/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141897959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rearing of Glossina morsitans morsitans tsetse flies for the sterile insect technique: evaluating the impact of irradiation and transportation during early and late-stage pupal development on the quality of emerging adults. 为昆虫不育技术饲养采采蝇：评估蛹早期和晚期发育过程中辐照和运输对新成虫质量的影响。

IF 2.3 2区医学

Parasite Pub Date : 2024-01-01 Epub Date: 2024-11-21 DOI: 10.1051/parasite/2024068

Caroline K Mirieri, Güler Demirbas Uzel, Andrew G Parker, Jérémy Bouyer, Linda De Vooght, Vera I D Ros, Monique M van Oers, Adly M M Abd-Alla

{"title":"Rearing of Glossina morsitans morsitans tsetse flies for the sterile insect technique: evaluating the impact of irradiation and transportation during early and late-stage pupal development on the quality of emerging adults.","authors":"Caroline K Mirieri, Güler Demirbas Uzel, Andrew G Parker, Jérémy Bouyer, Linda De Vooght, Vera I D Ros, Monique M van Oers, Adly M M Abd-Alla","doi":"10.1051/parasite/2024068","DOIUrl":"10.1051/parasite/2024068","url":null,"abstract":"Human African trypanosomiasis (HAT) and African animal trypanosomosis (AAT) are devastating diseases spread by tsetse flies (Glossina spp.), affecting humans and livestock, respectively. Current efforts to manage these diseases by eliminating the vector through the sterile insect technique (SIT) require transportation of irradiated late-stage tsetse pupae under chilling, which has been reported to reduce the biological quality of emerged flies. We therefore evaluated the impact of irradiation and transportation (including vibration and shock) on pupae at early-stage development (22 days of age) under ambient temperature and compared it to that on pupae at the late-stage development (29 days of age) under chilling, the current practice for tsetse in SIT programs. The quality of flies emerging from these transported pupae was assessed by their emergence rates, flight propensity, mating ability, insemination rates and survival rates (over ca. 100 days, and after specified shorter periods). Generally, flies emerging from the 22-day-old pupae had significantly (p < 0.05) higher values for the tested quality parameters, as compared to those emerging from 29-day-old pupae. Irradiation, transportation and the combination thereof significantly (p < 0.05) reduced all the tested quality parameters as compared with the untreated control within the 22-day-old pupae group. Further, vibration had a significant negative effect on the quality of flies, notwithstanding the age of the pupae. Irradiation and transportation of pupae at 22 days of age resulted in a higher proportion of flies of good biological quality as compared to those of 29 days of age, and hence may be considered for future SIT programs.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"31 ","pages":"73"},"PeriodicalIF":2.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11583636/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142688421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Relationship between acaricide resistance and acetylcholinesterase gene polymorphisms in the cattle tick Rhipicephalus microplus. 牛蜱 Rhipicephalus microplus 的杀螨剂抗性与乙酰胆碱酯酶基因多态性之间的关系。

IF 2.9 2区医学

Parasite Pub Date : 2024-01-01 Epub Date: 2024-02-04 DOI: 10.1051/parasite/2024003

Raquel Cossio-Bayugar, Francisco Martinez-Ibañez, Hugo Aguilar-Diaz, Estefan Miranda-Miranda

{"title":"Relationship between acaricide resistance and acetylcholinesterase gene polymorphisms in the cattle tick Rhipicephalus microplus.","authors":"Raquel Cossio-Bayugar, Francisco Martinez-Ibañez, Hugo Aguilar-Diaz, Estefan Miranda-Miranda","doi":"10.1051/parasite/2024003","DOIUrl":"10.1051/parasite/2024003","url":null,"abstract":"In this study, we aimed to develop a comprehensive methodology for identifying amino acid polymorphisms in acetylcholinesterase transcript 2 (AChE2) in acaricide-resistant Rhipicephalus microplus ticks. This included assessing AChE2 expression levels through qPCR and conducting 3D modeling to evaluate the interaction between acaricides and AChE2 using docking techniques. The study produced significant results, demonstrating that acaricide-resistant R. microplus ticks exhibit significantly higher levels of AChE expression than susceptible reference ticks. In terms of amino acid sequence, we identified 9 radical amino acid substitutions in AChE2 from acaricide-resistant ticks, when compared to the gene sequence of the susceptible reference strain. To further understand the implications of these substitutions, we utilized 3D acaricide-AChE2 docking modeling to examine the interaction between the acaricide and the AChE2 catalytic site. Our models suggest that these amino acid polymorphisms alter the configuration of the binding pocket, thereby contributing to differences in acaricide interactions and ultimately providing insights into the acaricide-resistance phenomenon in R. microplus.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"31 ","pages":"3"},"PeriodicalIF":2.9,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10840460/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139692588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Occurrence and genotyping of Enterocytozoon bieneusi in flying squirrels (Trogopterus xanthipes) from China. 中国鼯鼠（Trogopterus xanthipes）肠道酵母菌（Enterocytozoon bieneusi）的出现和基因分型。

IF 2.3 2区医学

Parasite Pub Date : 2024-01-01 Epub Date: 2024-07-04 DOI: 10.1051/parasite/2024037

Xuehan Liu, Chi Zhang, Tiantian Li, Xiaojing Xia, Yanzhao Xu, Jianhe Hu, Longxian Zhang, Lei Wang, Meng Qi

{"title":"Occurrence and genotyping of Enterocytozoon bieneusi in flying squirrels (Trogopterus xanthipes) from China.","authors":"Xuehan Liu, Chi Zhang, Tiantian Li, Xiaojing Xia, Yanzhao Xu, Jianhe Hu, Longxian Zhang, Lei Wang, Meng Qi","doi":"10.1051/parasite/2024037","DOIUrl":"10.1051/parasite/2024037","url":null,"abstract":"Enterocytozoon bieneusi is an obligate intracellular microsporidian parasite with a worldwide distribution. As a zoonotic pathogen, E. bieneusi can infect a wide range of wildlife hosts through the fecal-oral route. Although the feces of flying squirrels (Trogopterus xanthipes) are considered a traditional Chinese medicine (as \"faeces trogopterori\"), no literature is available on E. bieneusi infection in flying squirrels to date. In this study, a total of 340 fresh flying squirrel fecal specimens from two captive populations were collected in Pingdingshan city, China, to detect the prevalence of E. bieneusi and assess their zoonotic potential. By nested PCR amplification of the ITS gene, six specimens tested positive, with positive samples from each farm, with an overall low infection rate of 1.8%. The ITS sequences revealed three genotypes, including known genotype D and two novel genotypes, HNFS01 and HNFS02. Genotype HNFS01 was the most prevalent (4/6, 66.7%). Phylogenetic analysis showed that all genotypes clustered into zoonotic Group 1, with the novel genotypes clustering into different subgroups. To our knowledge, this is the first report of E. bieneusi infection in flying squirrels, suggesting that flying squirrels could act as a potential reservoir and zoonotic threat for E. bieneusi transmission to humans in China.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"31 ","pages":"37"},"PeriodicalIF":2.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11223590/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141498644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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