Parasite最新文献_第5页

Anisakid parasite diversity in a pygmy sperm whale, Kogia breviceps (Cetacea: Kogiidae) stranded at the edge of its distribution range in the Northeast Atlantic Ocean. 搁浅在大西洋东北部分布区边缘的侏儒抹香鲸 Kogia breviceps（鲸目动物：Kogiidae）体内的 Anisakid 寄生虫多样性。

IF 2.3 2区医学

Parasite Pub Date : 2024-01-01 Epub Date: 2024-07-30 DOI: 10.1051/parasite/2024042

Paolo Cipriani, Marialetizia Palomba, Lucilla Giulietti, Renato Aco-Alburqueque, Roberta Andolfi, Mariel Ten Doeschate, Andrew Brownlow, Nicholas J Davison, Simonetta Mattiucci

{"title":"Anisakid parasite diversity in a pygmy sperm whale, Kogia breviceps (Cetacea: Kogiidae) stranded at the edge of its distribution range in the Northeast Atlantic Ocean.","authors":"Paolo Cipriani, Marialetizia Palomba, Lucilla Giulietti, Renato Aco-Alburqueque, Roberta Andolfi, Mariel Ten Doeschate, Andrew Brownlow, Nicholas J Davison, Simonetta Mattiucci","doi":"10.1051/parasite/2024042","DOIUrl":"10.1051/parasite/2024042","url":null,"abstract":"Anisakid nematodes are a globally distributed group of marine mammal parasites. Kogiid whales, including the pygmy sperm whale Kogia breviceps, host an assemblage of specific anisakid species. Currently, three species are known to be specific to kogiid hosts, i.e., Skrjabinisakis paggiae, S. brevispiculata, and the less studied Pseudoterranova ceticola. The aim of this study was to investigate the species diversity of anisakid nematodes sampled from a pygmy sperm whale stranded in 2013 at the edge of its distribution range in the Northeast Atlantic, specifically in the North of Scotland. Nematodes were assigned to genus level based on morphology and identified by sequence analysis of the mtDNA cox2 gene and the rDNA ITS region. The present finding represents the first observation of syntopic occurrence of adult stages of S. brevispiculata, S. paggiae, and P. ceticola in a pygmy sperm whale in the Northeast Atlantic, and represent the northernmost record of these species in this area. Skrjabinisakis brevispiculata was the most abundant species, accounting for 55% of the identified nematodes, predominantly in the adult stage. Anisakis simplex (s.s.) was also abundant, with most specimens in the preadult stage, followed by S. paggiae and P. ceticola. The pygmy sperm whale is rarely documented in Scottish waters, and its occurrence in the area could suggest expansion of its geographic range. The presence of S. brevispiculata, S. paggiae, and P. ceticola in this whale species in this region may indicate a shift in the whole host community involved in the life cycle of these parasites in northern waters. However, it is also plausible that these parasites were acquired while the whale was feeding in more southern regions, before migrating northbound.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"31 ","pages":"43"},"PeriodicalIF":2.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11290333/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141856150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantitative phosphoproteomic analysis of chicken DF-1 cells infected with Eimeria tenella, using tandem mass tag (TMT) and parallel reaction monitoring (PRM) mass spectrometry. 利用串联质量标记（TMT）和平行反应监测（PRM）质谱对感染了天牛埃默氏菌的鸡 DF-1 细胞进行定量磷蛋白组学分析。

IF 2.9 2区医学

Parasite Pub Date : 2024-01-01 Epub Date: 2024-05-17 DOI: 10.1051/parasite/2024027

Liu-Shu Jia, Zhan Liu, Shun-Hai Zhu, Qi-Ping Zhao, Hong-Yu Han, Huan-Zhi Zhao, Yu Yu, Hui Dong

{"title":"Quantitative phosphoproteomic analysis of chicken DF-1 cells infected with Eimeria tenella, using tandem mass tag (TMT) and parallel reaction monitoring (PRM) mass spectrometry.","authors":"Liu-Shu Jia, Zhan Liu, Shun-Hai Zhu, Qi-Ping Zhao, Hong-Yu Han, Huan-Zhi Zhao, Yu Yu, Hui Dong","doi":"10.1051/parasite/2024027","DOIUrl":"https://doi.org/10.1051/parasite/2024027","url":null,"abstract":"Eimeria tenella is an obligate intracellular parasite which causes great harm to the poultry breeding industry. Protein phosphorylation plays a vital role in host cell-E. tenella interactions. However, no comprehensive phosphoproteomic analyses of host cells at various phases of E. tenella infection have been published. In this study, quantitative phosphoproteomic analysis of chicken embryo DF-1 fibroblasts that were uninfected (UI) or infected with E. tenella for 6 h (PI6, the early invasion phase) or 36 h (PI36, the trophozoite development phase) was conducted. A total of 10,122 phosphopeptides matched to 3,398 host cell phosphoproteins were identified and 13,437 phosphorylation sites were identified. Of these, 491, 1,253, and 275 differentially expressed phosphorylated proteins were identiﬁed in the PI6/UI, PI36/UI, and PI36/PI6 comparisons, respectively. KEGG pathway enrichment analysis showed that E. tenella modulated host cell processes through phosphorylation, including focal adhesion, regulation of the actin cytoskeleton, and FoxO signaling to support its early invasion phase, and modulating adherens junctions and the ErbB signaling pathway to favor its trophozoite development. These results enrich the data on the interaction between E. tenella and host cells and facilitate a better understanding of the molecular mechanisms underlying host-parasite relationships.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"31 ","pages":"23"},"PeriodicalIF":2.9,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11101204/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140958703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Annexin gene family in Spirometra mansoni (Cestoda: Diphyllobothriidae) and its phylogenetic pattern among Platyhelminthes of medical interest. 曼氏螺旋绦虫（绦虫纲：Diphyllobothriidae）的 Annexin 基因家族及其在具有医学意义的扁形动物中的系统发育模式。

IF 2.3 2区医学

Parasite Pub Date : 2024-01-01 Epub Date: 2024-06-21 DOI: 10.1051/parasite/2024034

Xiao Yi Su, Fei Gao, Si Yao Wang, Jing Li, Zhong Quan Wang, Xi Zhang

{"title":"Annexin gene family in Spirometra mansoni (Cestoda: Diphyllobothriidae) and its phylogenetic pattern among Platyhelminthes of medical interest.","authors":"Xiao Yi Su, Fei Gao, Si Yao Wang, Jing Li, Zhong Quan Wang, Xi Zhang","doi":"10.1051/parasite/2024034","DOIUrl":"10.1051/parasite/2024034","url":null,"abstract":"The plerocercoid larvae of Spirometra mansoni are etiological agents of human and animal sparganosis. Annexins are proteins with important roles in parasites. However, our knowledge of annexins in S. mansoni is still inadequate. In this study, 18 new members of the Annexin (ANX) family were characterized in S. mansoni. The clustering analysis demonstrated that all the SmANXs were divided into two main classes, consistent with the patterns of conserved motif organization. The 18 SmANXs were detected at all developmental stages (plerocercoid, adult, and egg) and displayed ubiquitous but highly variable expression patterns in all tissues/organs studied. The representative member rSmANX18 was successfully cloned and expressed. The protein was immunolocalized in the tegument and parenchyma of the plerocercoid and in the tegument, parenchyma, uterus and egg shell of adult worms. The recombinant protein can bind phospholipids with high affinity in a Ca2+-dependent manner, shows high anticoagulant activity and combines with FITC to recognize apoptotic cells. Annexin gene polymorphism and conservative core motif permutation were found in both cestodes and trematodes. SmANXs also revealed high genetic diversity among Platyhelminthes of medical interest. Our findings lay a foundation for further studies on the biological functions of ANXs in S. mansoni as well as other taxa in which ANXs occur.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"31 ","pages":"32"},"PeriodicalIF":2.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11195529/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141443171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cross-species transmission of Cryptosporidium in wild rodents from the southern region of Zhejiang Province of China and its possible impact on public health. 中国浙江省南部地区野生啮齿动物隐孢子虫的跨物种传播及其对公共卫生的可能影响。

IF 2.3 2区医学

Parasite Pub Date : 2024-01-01 Epub Date: 2024-07-01 DOI: 10.1051/parasite/2024033

Yanyan Jiang, Aiying Jiang, Guangxu Ren, Long Wang, Xianming Xin, Zhongying Yuan, Jiani Liu, Zhen Li, Yanbin Sun, Shanshan Zhou, Gang Lu, Huicong Huang, Wei Zhao

{"title":"Cross-species transmission of Cryptosporidium in wild rodents from the southern region of Zhejiang Province of China and its possible impact on public health.","authors":"Yanyan Jiang, Aiying Jiang, Guangxu Ren, Long Wang, Xianming Xin, Zhongying Yuan, Jiani Liu, Zhen Li, Yanbin Sun, Shanshan Zhou, Gang Lu, Huicong Huang, Wei Zhao","doi":"10.1051/parasite/2024033","DOIUrl":"10.1051/parasite/2024033","url":null,"abstract":"Wild rodents serve as reservoirs for Cryptosporidium and are overpopulated globally. However, genetic data regarding Cryptosporidium in these animals from China are limited. Here, we have determined the prevalence and genetic characteristics of Cryptosporidium among 370 wild rodents captured from three distinct locations in the southern region of Zhejiang Province, China. Fresh feces were collected from the rectum of each rodent, and DNA was extracted from them. The rodent species was identified by PCR amplifying the vertebrate cytochrome b gene. Cryptosporidium was detected by PCR amplification and amplicon sequencing the small subunit of ribosomal RNA gene. Positive samples of C. viatorum and C. parvum were further subtyped by analyzing the 60-kDa glycoprotein gene. A positive Cryptosporidium result was found in 7% (26/370) of samples, involving five rodent species: Apodemus agrarius (36), Niviventer niviventer (75), Rattus losea (18), R. norvegicus (155), and R. tanezumi (86). Their respective Cryptosporidium positive rates were 8.3%, 5.3%, 11.1%, 7.1%, and 7.0%. Sequence analysis confirmed the presence of three Cryptosporidium species: C. parvum (4), C. viatorum (1), and C. muris (1), and two genotypes: Cryptosporidium rat genotype IV (16) and C. mortiferum-like (4). Additionally, two subtypes of C. parvum (IIdA15G1 and IIpA19) and one subtype of C. viatorum (XVdA3) were detected. These results demonstrate that various wild rodent species in Zhejiang were concurrently infected with rodent-adapted and zoonotic species/genotypes of Cryptosporidium, indicating that these rodents can play a role in maintaining and dispersing this parasite into the environment and other hosts, including humans.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"31 ","pages":"34"},"PeriodicalIF":2.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11216159/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141469992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cryptosporidium spp. in captive snakes from 26 provinces in China: Prevalence, molecular characterization, and symptoms. 中国 26 个省份人工饲养蛇类中的隐孢子虫属：流行率、分子特征和症状。

IF 2.3 2区医学

Parasite Pub Date : 2024-01-01 Epub Date: 2024-08-07 DOI: 10.1051/parasite/2024047

Yilei Zhang, Zhenxiao Lu, Lingru He, Guodong Xiao, Lijie Tian, Jiawei Zhu, Tian Liu, Qiangxin Ou, Haibo Chen, Yew Hwong, Yangjun Kang, Qianming Xu, Qingxun Zhang, Congshan Yang

{"title":"Cryptosporidium spp. in captive snakes from 26 provinces in China: Prevalence, molecular characterization, and symptoms.","authors":"Yilei Zhang, Zhenxiao Lu, Lingru He, Guodong Xiao, Lijie Tian, Jiawei Zhu, Tian Liu, Qiangxin Ou, Haibo Chen, Yew Hwong, Yangjun Kang, Qianming Xu, Qingxun Zhang, Congshan Yang","doi":"10.1051/parasite/2024047","DOIUrl":"10.1051/parasite/2024047","url":null,"abstract":"Snakes are sometimes regarded as pets and are used in traditional Chinese medicine. Cryptosporidium spp. are frequently identified in snakes, representing an important pathogen and causing gastrointestinal diseases. Current data indicate that risk factors for infection and patterns of clinical symptom presentation may differ among Cryptosporidium spp. To better understand the infection status by Cryptosporidium spp., fecal samples were collected from 603 asymptomatic and 147 symptomatic snakes in 26 provinces of China. These samples came from Elaphe guttata, Elaphe obsoleta, Pituophis melanoleucus, Thamnophis sirtalis, Lampropeltis getulus, and Heterodon nasicus. The partial small subunit (SSU) rRNA gene was amplified using nested polymerase chain reaction (PCR) to investigate the infection rate of Cryptosporidium spp., and to assess evolutionary relationships and genetic characterization. A prevalence of 20% was recorded in asymptomatic snakes, with age identified as a significant risk factor. In contrast, 70% of symptomatic snakes were positive for Cryptosporidium spp., with Cryptosporidium serpentis and Cryptosporidium varanii (syn. C. saurophilum). Further analysis revealed a potential association between C. serpentis and regurgitation, and C. varanii and diarrhea, while neither species was linked to flatulence. To our knowledge, this is the first study to report Cryptosporidium spp. and associated clinical signs in symptomatic snakes in China. This study aims to enhance the understanding of Cryptosporidium infections, risk factors, and clinical manifestations in snakes, providing data crucial for the control and prevention of cryptosporidiosis.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"31 ","pages":"47"},"PeriodicalIF":2.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11305116/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141897957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A comparative study of the microbiomes of the ticks Rhipicephalus microplus and Hyalomma anatolicum. 微头蜱和鸭眼蜱微生物组的比较研究。

IF 2.3 2区医学

Parasite Pub Date : 2024-01-01 Epub Date: 2024-11-28 DOI: 10.1051/parasite/2024074

Adeel Mumtaz Abbasi, Shiza Nasir, Amna Arshad Bajwa, Haroon Akbar, Muhammad Muddassir Ali, Muhammad Imran Rashid

{"title":"A comparative study of the microbiomes of the ticks Rhipicephalus microplus and Hyalomma anatolicum.","authors":"Adeel Mumtaz Abbasi, Shiza Nasir, Amna Arshad Bajwa, Haroon Akbar, Muhammad Muddassir Ali, Muhammad Imran Rashid","doi":"10.1051/parasite/2024074","DOIUrl":"10.1051/parasite/2024074","url":null,"abstract":"Hyalomma anatolicum and Rhipicephalus microplus are tick species that are important vectors of numerous pathogens affecting both humans and livestock. Endosymbionts, such as Coxiella-like endosymbionts (CLE), Francisella-like endosymbionts (FLE), and Candidatus Midichloria, play a crucial role in the physiology and vector competence of these ticks. In this study, we investigated the microbial composition of H. anatolicum and R. microplus from four geographically distinct regions of Pakistan to assess whether environmental differences influence their microbiomes. We analyzed the ticks' gut microbiome targeting the V3-V4 hypervariable region of 16S rRNA for Illumina 16S metagenome NGS sequencing and processed overall 144 ticks. Analysis of gut bacterial composition resulted in observation of 1200 R. microplus and 968 H. anatolicum unique amplicon sequencing variants (ASVs). Relative abundance, Alpha diversity (Shannon, Faith's phylogenetic distance) and beta diversity metrics (Bray-Curtis, Jaccard and UniFrac) were analyzed and revealed that H. anatolicum ticks have significantly unique and diverse microbial communities with Acinetobacter indicus and Francisella-like endosymbionts dominating as opposed to Candidatus Midichloria. Rhipicephalus microplus exhibited results consistent with the previous studies with no major changes in microbiome including Coxiella-like endosymbionts as the major contributor. These findings suggest that geographical and environmental factors play a significant role in shaping the tick microbiome, with potential consequences for disease transmission and tick survivability. Further research is needed to elucidate the functional roles of these microbial shifts and their impact on public health and livestock in affected regions.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"31 ","pages":"74"},"PeriodicalIF":2.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11604214/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142751374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High genetic diversity of Enterocytozoon bieneusi in minks and raccoon dogs in northern China. 中国北方水貂和貉子肠虫的高度遗传多样性。

IF 2.3 2区医学

Parasite Pub Date : 2024-01-01 Epub Date: 2024-11-19 DOI: 10.1051/parasite/2024071

Nian-Yu Xue, Zhong-Yuan Li, Hai-Tao Wang, Ya Qin, Xue-Min Li, Qing-Yu Hou, Jing Jiang, Xing Yang, Hong-Bo Ni

{"title":"High genetic diversity of Enterocytozoon bieneusi in minks and raccoon dogs in northern China.","authors":"Nian-Yu Xue, Zhong-Yuan Li, Hai-Tao Wang, Ya Qin, Xue-Min Li, Qing-Yu Hou, Jing Jiang, Xing Yang, Hong-Bo Ni","doi":"10.1051/parasite/2024071","DOIUrl":"10.1051/parasite/2024071","url":null,"abstract":"Enterocytozoon bieneusi, a zoonotic pathogen prevalent in both humans and animals, is the most frequently diagnosed microsporidian species in humans and presents significant public health risks. However, data on the prevalence and genotypes of E. bieneusi in farmed minks (Neovison vison) and raccoon dogs (Nyctereutes procyonoides) in China are limited. Therefore, 275 minks (89 from Hebei Province, 57 from Heilongjiang Province, 109 from Liaoning Province, 20 from Shandong Province) and 235 raccoon dogs (114 from Hebei Province, 27 from Heilongjiang Province, 61 from Liaoning Province, 33 from Jilin Province) were examined for the prevalence and genotypes of E. bieneusi through sequence analysis of the internal transcribed spacer (ITS) region of the rRNA gene. The overall prevalence of E. bieneusi was 18.6% (95/510), with 10.5% (29/275) in farmed minks and 28.1% (66/235) in raccoon dogs. Ten genotypes (CHN-F1, genotype D, Type IV, EbpC, NCF2, NCF5, NCF6, Peru8, Henan V, and MJ5) were identified in minks and raccoon dogs. This study is the first to detect the CHN-F1, NCF2, NCF6, Peru8, and Henan V genotypes in minks and the NCF5, NCF6, and MJ5 genotypes in raccoon dogs. Additionally, the D, Type IV, and Peru8 genotypes, previously identified in humans, were also found in minks and raccoon dogs, suggesting that these animals could be potential sources of human microsporidiosis. These findings expand the understanding of E. bieneusi's host distribution in China and contribute to the prevention of zoonotic E. bieneusi infections among farmed animals.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"31 ","pages":"71"},"PeriodicalIF":2.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11575893/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142676429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tick-borne Apicomplexa in wildlife and ticks of French Guiana. 法属圭亚那野生动物和蜱虫中的蜱传Apicomplexa。

IF 2.3 2区医学

Parasite Pub Date : 2024-01-01 Epub Date: 2024-08-15 DOI: 10.1051/parasite/2024052

Rachid Koual, Benoit de Thoisy, Xavier Baudrimont, Stéphane Garnier, Frédéric Delsuc, Olivier Duron

引用次数: 0

Diversity of biting midges, mosquitoes and sand flies at four dog shelters in rural and peri-urban areas of Central Morocco. 摩洛哥中部农村和城郊地区四个狗收容所中咬螨、蚊子和沙蝇的多样性。

IF 2.3 2区医学

Parasite Pub Date : 2024-01-01 Epub Date: 2024-09-27 DOI: 10.1051/parasite/2024057

Abderrahmane Zahri, Mehdi Ahlamine, Fatima-Zahra Abou-Elaaz, Hasnaa Talimi, Ikhlass El Berbri, Thomas Balenghien, Maria Bourquia

{"title":"Diversity of biting midges, mosquitoes and sand flies at four dog shelters in rural and peri-urban areas of Central Morocco.","authors":"Abderrahmane Zahri, Mehdi Ahlamine, Fatima-Zahra Abou-Elaaz, Hasnaa Talimi, Ikhlass El Berbri, Thomas Balenghien, Maria Bourquia","doi":"10.1051/parasite/2024057","DOIUrl":"https://doi.org/10.1051/parasite/2024057","url":null,"abstract":"Blood-feeding arthropods are involved in the transmission of several pathogens that have a major impact on public health. Entomological investigations highlighted the composition, abundance, and diversity of flying hematophagous arthropods at four dog shelters located in central Morocco during an eight-month study, with the aim of discussing their vectorial roles and assessing the risk of these shelters as foci for zoonotic diseases. Monitoring of the arthropod fauna for 64 catch nights resulted in the collection of 2,321 biting midges (Ceratopogonidae), 570 mosquitoes (Culicidae), and 475 sand flies (Psychodidae). Fourteen Culicoides species were recorded and dominant species were Culicoides imicola (55.96%), C. paolae (16.07%), C. circumscriptus (10.29%), and C. newsteadi (5.77%). Three mosquito species were collected, including Culex pipiens s.l. (96.84%), Culiseta longiareolata (2.80%), and Cx. perexiguus (0.36%). Ten sand fly species were collected, including seven Phlebotomus species (62.70%) and three Sergentomyia species (37.30%); Sergentomyia minuta was the most dominant species (34.31%), followed by Phlebotomus sergenti (32.42%), typical Ph. perniciosus (8.63%), Ph. alexandri (6.94%), and Ph. riouxi (6.52%). The coexistence of several vectors in these study areas indicates the potential circulation of a wide range of pathogens, including zoonotic ones, thus requiring the implementation of surveillance and control programs to prevent the emergence and spread of disease outbreaks.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"31 ","pages":"57"},"PeriodicalIF":2.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11433837/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142351526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabarcoding of protozoa and helminth in black-necked cranes: a high prevalence of parasites and free-living amoebae. 黑颈鹤体内原生动物和蠕虫的代谢编码：寄生虫和自由生活变形虫的高流行率。

IF 2.3 2区医学

Parasite Pub Date : 2024-01-01 Epub Date: 2024-05-30 DOI: 10.1051/parasite/2024028

Mengshi Yu, Wenhao Li, Xin He, Guiwen He, Yonfang Yao, Yuanjian Wang, Mingcui Shao, Tingsong Xiong, Huailiang Xu, Junsong Zhao

{"title":"Metabarcoding of protozoa and helminth in black-necked cranes: a high prevalence of parasites and free-living amoebae.","authors":"Mengshi Yu, Wenhao Li, Xin He, Guiwen He, Yonfang Yao, Yuanjian Wang, Mingcui Shao, Tingsong Xiong, Huailiang Xu, Junsong Zhao","doi":"10.1051/parasite/2024028","DOIUrl":"10.1051/parasite/2024028","url":null,"abstract":"Parasites and free-living amoebae (FLA) are common pathogens that pose threats to wildlife and humans. The black-necked crane (Grus nigricollis) is a near-threatened species and there is a shortage of research on its parasite diversity. Our study aimed to use noninvasive methods to detect intestinal parasites and pathogenic FLA in G. nigricollis using high-throughput sequencing (HTS) based on the 18S rDNA V9 region. A total of 38 fresh fecal samples were collected in Dashanbao, China, during the overwintering period (early-, middle I-, middle II-, and late-winter). Based on the 18S data, eight genera of parasites were identified, including three protozoan parasites: Eimeria sp. (92.1%) was the dominant parasite, followed by Tetratrichomonas sp. (36.8%) and Theileria sp. (2.6%). Five genera of helminths were found: Echinostoma sp. (100%), Posthodiplostomum sp. (50.0%), Euryhelmis sp. (26.3%), Eucoleus sp. (50.0%), and Halomonhystera sp. (2.6%). Additionally, eight genera of FLA were detected, including the known pathogens Acanthamoeba spp. (n = 13) and Allovahlkampfia spp. (n = 3). Specific PCRs were used to further identify the species of some parasites and FLA. Furthermore, the 18S data indicated significant changes in the relative abundance and genus diversity of the protozoan parasites and FLA among the four periods. These results underscore the importance of long-term monitoring of pathogens in black-necked cranes to protect this near-endangered species.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"31 ","pages":"28"},"PeriodicalIF":2.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11141520/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141180360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0