Parasite最新文献_第6页

Biological characteristics and functions of a novel glutamate dehydrogenase from Trichinella spiralis. 螺旋毛癣菌新型谷氨酸脱氢酶的生物学特征和功能

IF 2.3 2区医学

Parasite Pub Date : 2024-01-01 Epub Date: 2024-10-28 DOI: 10.1051/parasite/2024065

Yong Kang Cheng, Yao Zhang, Zhao Yu Zhang, Pei Kun Cong, Ji Yu Feng, Ru Zhang, Shao Rong Long, Xi Zhang, Zhong Quan Wang, Jing Cui

{"title":"Biological characteristics and functions of a novel glutamate dehydrogenase from Trichinella spiralis.","authors":"Yong Kang Cheng, Yao Zhang, Zhao Yu Zhang, Pei Kun Cong, Ji Yu Feng, Ru Zhang, Shao Rong Long, Xi Zhang, Zhong Quan Wang, Jing Cui","doi":"10.1051/parasite/2024065","DOIUrl":"10.1051/parasite/2024065","url":null,"abstract":"Glutamate dehydrogenase (GDH) plays an important role in the metabolism of organisms. Its high abundance in mitochondria in particular highlights its core role in cellular physiological processes. GDH catalyzes the mutual conversion between L-glutamic acid and α-ketoglutaric acids. At the same time, this transformation is accompanied by the oxidation-reduction of NAD(H) or NADP(H). This process not only helps to link amino acid metabolism with sugar metabolism, but also helps maintain the balance of intracellular pH and nitrogen homeostasis. In this study, a novel Trichinella spiralis glutamate dehydrogenase (TsGDH) was cloned, expressed and identified. The results revealed that TsGDH was expressed at various stages of development of the nematode T. spiralis, with higher expression levels in the adult worm stage, and was mainly localized in the cuticle, muscular layer, stichosome and female intrauterine embryos. After RNAi treatment, larval natural TsGDH enzyme activity was obviously reduced, and metabolism, molting, growth and reproduction were also significantly inhibited. The results indicate that TsGDH plays an important role in the development and survival of T. spiralis, and it may be a potential molecular target of anti-Trichinella vaccines and drugs.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"31 ","pages":"65"},"PeriodicalIF":2.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11514599/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142505373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Culicoides and midge-associated arboviruses on cattle farms in Yunnan Province, China. 中国云南省养牛场的螨类和蠓类相关虫媒病毒。

IF 2.3 2区医学

Parasite Pub Date : 2024-01-01 Epub Date: 2024-11-19 DOI: 10.1051/parasite/2024072

Ying-Liang Duan, Zhan-Hong Li, Glenn A Bellis, Le Li, Bing-Gang Liu, Jian-Ping Wang, Jian-Mei Liu, De-Fang Liao, Jian-Bo Zhu

{"title":"Culicoides and midge-associated arboviruses on cattle farms in Yunnan Province, China.","authors":"Ying-Liang Duan, Zhan-Hong Li, Glenn A Bellis, Le Li, Bing-Gang Liu, Jian-Ping Wang, Jian-Mei Liu, De-Fang Liao, Jian-Bo Zhu","doi":"10.1051/parasite/2024072","DOIUrl":"10.1051/parasite/2024072","url":null,"abstract":"Culicoides spp. (Diptera: Ceratopogonidae) are small biting midges, some of which are the vectors of arboviruses affecting livestock, i.e., African horse sickness virus (AHSV), bluetongue virus (BTV), and epizootic hemorrhagic disease virus (EHDV). Yunnan Province, located in southwestern China, has a history of high prevalence of arboviruses. The diversity and abundance of Culicoides was observed between March 2022 and March 2023 on two cattle farms in Lufeng County of Yunnan Province, China and virus isolation and PCR detection were attempted from cattle blood and Culicoides spp. collected from the farms. Light trap collections contained 19 species of Culicoides belonging to 8 subgenera and one unplaced species group and were dominated by C. oxystoma (63.4%), C. imicola (16.2%), C. arakawae (13.4%), C. sp. near newsteadi (2.3%), and C. orientalis (1.7%). A total of 8,343 Culicoides were used for statistical analysis; from these collections 997 Culicoides specimens belonging to 10 species were screened for the presence of BTV, EHDV, Yunnan Orbivirus (YUOV), and Yongshan totivirus (YSToV) using reverse transcription quantitative PCR (RT-qPCR). One strain of YUOV was isolated from cattle blood and 7 isolates of YSToV were isolated from 5 different species of Culicoides. BTV and YSToV were detected from 2 and 3 pools of parous C. imicola specimens by RT-qPCR, respectively, which is the first report of a totivirus to be associated with Culicoides. Culicoides imicola is likely to be the major vector of Culicoides-borne arboviruses in Lufeng County, which is a relatively dry locality, and adult C. imicola may play a role of BTV overwintering.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"31 ","pages":"72"},"PeriodicalIF":2.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11578047/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142676425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Alobophora sandrae n. gen. n. sp. (Digenea: Caballerotrematidae) infecting Arapaima gigas sensu lato (Osteoglossiformes: Arapaimidae) with a revision of Caballerotrema, key to Caballerotrematidae, and updated phylogeny. Alobophora sandrae n. gen. n. sp. (Digenea: Caballerotrematidae) infecting Arapaima gigas sensu lato (Osteoglossiformes: Arapaimidae) with a revision of Caballerotrema, key to Caballerotrematidae, and updated phylogeny.

IF 2.3 2区医学

Parasite Pub Date : 2024-01-01 Epub Date: 2024-09-23 DOI: 10.1051/parasite/2024054

Kamila Cajiao-Mora, John H Brule, Micah B Warren, Steven P Ksepka, Haley R Dutton, Stephen A Bullard

{"title":"Alobophora sandrae n. gen. n. sp. (Digenea: Caballerotrematidae) infecting Arapaima gigas sensu lato (Osteoglossiformes: Arapaimidae) with a revision of Caballerotrema, key to Caballerotrematidae, and updated phylogeny.","authors":"Kamila Cajiao-Mora, John H Brule, Micah B Warren, Steven P Ksepka, Haley R Dutton, Stephen A Bullard","doi":"10.1051/parasite/2024054","DOIUrl":"10.1051/parasite/2024054","url":null,"abstract":"We propose and describe Alobophora sandrae Cajiao-Mora & Bullard n. gen., n. sp. (Digenea: Caballerotrematidae) for specimens we collected from arapaima, Arapaima gigas sensu lato (Osteoglossiformes: Arapaimidae) in the Amazon River near Leticia, Colombia. Alobophora differs from Caballerotrema Prudhoe, 1960 by lacking head collar projections and by having clustered corner spines and a narrow head collar (4-5× wider than pharynx), whereas Caballerotrema has head collar projections, lacks clustered corner spines, and has a broad head collar (7-8× wider than pharynx). We reassign Caballerotrema annulatum (Diesing, 1850) Ostrowski de Núñez & Sattmann, 2002 to the new genus, as Alobophora annulata (Diesing, 1850) Cajiao-Mora and Bullard n. comb., and provide a supplemental description of Caballerotrema brasiliense Prudhoe, 1960 based on specimens we collected from arapaima. We also examined the holotype and a paratype of Caballerotrema piscicola (Stunkard, 1960) Kostadinova & Gibson, 2001 and concluded that C. piscicola is a junior subjective synonym of C. brasiliense. Our 28S phylogeny recovered A. sandrae sister to A. annulata, with that clade sister to a clade comprising C. brasiliense and an innominate species of Caballerotrema. Caballerotrematidae was recovered sister to Echinostomatidae. We also provide a dichotomous key to caballerotrematids based on head collar projections, corner spine arrangement, proportional pharynx and head collar breadth, testes shape and arrangement, body surface spine shape and distribution, vitellarium distribution, and abundance of prostatic cells.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"31 ","pages":"55"},"PeriodicalIF":2.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11418392/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142292837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular prevalence and subtype distribution of Blastocystis spp. among children who have diarrheia or are asymptomatic in Wenzhou, Zhejiang Province, China. 中国浙江省温州市腹泻或无症状儿童中子囊虫的分子流行率和亚型分布。

IF 2.9 2区医学

Parasite Pub Date : 2024-01-01 Epub Date: 2024-03-06 DOI: 10.1051/parasite/2024012

Wei Zhao, Guangxu Ren, Long Wang, Lisha Xie, Jiayang Wang, Jialiang Mao, Yanbin Sun, Gang Lu, Huicong Huang

{"title":"Molecular prevalence and subtype distribution of Blastocystis spp. among children who have diarrheia or are asymptomatic in Wenzhou, Zhejiang Province, China.","authors":"Wei Zhao, Guangxu Ren, Long Wang, Lisha Xie, Jiayang Wang, Jialiang Mao, Yanbin Sun, Gang Lu, Huicong Huang","doi":"10.1051/parasite/2024012","DOIUrl":"10.1051/parasite/2024012","url":null,"abstract":"Blastocystis sp., a significant zoonotic parasite with a global distribution, was the focus of this study, which aimed to investigate its prevalence and genetic diversity among diarrheic and asymptomatic children in Wenzhou, China. We collected 1,032 fecal samples from Yuying Children's Hospital, Wenzhou, China, comprising 684 from children with diarrhea and 348 from asymptomatic children. Genomic DNA extracted from these samples was used to detect Blastocystis spp. by PCR, targeting the small subunit ribosomal RNA gene. Subsequently, a phylogenetic tree was constructed, applying the maximum likelihood method. Blastocystis spp. were detected in 67 (6.5%) of the fecal samples. The prevalence rate of Blastocystis spp. in diarrheic children (8.8%; 60/684) was significantly higher than that in asymptomatic children (2.0%; 7/348) (χ 2 = 17.3, p < 0.001). Sequence analysis of the SSU rRNA gene identified five known Blastocystis spp. subtypes, ST1 (n = 12), ST2 (n = 5), ST3 (n = 35), ST4 (n = 12), and ST7 (n = 3). ST1 and ST3 were present in both diarrheic and asymptomatic children, while ST2, ST4, and ST7 were exclusive to diarrheic children. Intra-subtype genetic polymorphisms were identified, comprising four variations in ST1 (ST1-1 to ST1-4), five in ST3 (ST3-1 to ST3-5), two in ST4 (ST4-1 and ST4-2), and two in ST7 (ST7-1 and ST7-2). Notably, ST1-2 to ST1-4, ST3-3 to ST3-5, and ST7-1 and ST7-2 represent newly identified variations. The composition and genetic characteristics of subtypes among children in this region suggest various sources of infection, including human-to-human and animal-to-human transmission.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"31 ","pages":"12"},"PeriodicalIF":2.9,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10918642/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140050108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Population genetic structure of Schistosoma bovis and S. curassoni collected from cattle in Mali. 从马里牛身上采集的牛血吸虫和库拉索尼血吸虫的种群遗传结构。

IF 2.3 2区医学

Parasite Pub Date : 2024-01-01 Epub Date: 2024-07-02 DOI: 10.1051/parasite/2024035

Assitan Diakité, Privat Agniwo, Abdoulaye Dabo, Bakary Sidibé, Boris A E S Savassi, Ahristode Akplogan, Hassim Guindo, Laurent Dembélé, Moudachirou Ibikounlé, Safiatou Doumbo Niaré, Saidou Tembely, Jérôme Boissier

{"title":"Population genetic structure of Schistosoma bovis and S. curassoni collected from cattle in Mali.","authors":"Assitan Diakité, Privat Agniwo, Abdoulaye Dabo, Bakary Sidibé, Boris A E S Savassi, Ahristode Akplogan, Hassim Guindo, Laurent Dembélé, Moudachirou Ibikounlé, Safiatou Doumbo Niaré, Saidou Tembely, Jérôme Boissier","doi":"10.1051/parasite/2024035","DOIUrl":"10.1051/parasite/2024035","url":null,"abstract":"Schistosomiasis is of medical and veterinary importance. Despite the critical situation of schistosomiasis in sub-Saharan Africa, few molecular epidemiological studies have been carried out to determine the role of animals in its transmission. In Mali, it has been over three decades since the last molecular study of animal schistosomes was carried out. It is now urgent to identify circulating strains of the parasite because of potential interactions with other schistosome species, which could complicate disease control. The aim of our work was to study the composition and genetic structure of schistosome populations collected from cattle. The prevalence of schistosome was 23.9%, with the prevalences of Schistosoma bovis (Sb) and S. curassoni (Sc) estimated at 12.6% and 9.8%, respectively. No hybrid strains or S. haematobium were found. The parasites displayed distinct geographical distribution with Sb dominant in Bamako (78.8% and 98% in Central Bamako Slaughterhouse and Sabalibougou Slaughterhouses, respectively) and Sc dominant in Kayes (95.3%). Of the 476 parasites with a complete genetic profile, 60.4% were pure Sc, and were mainly from Kayes. We identified two clusters at the site level (Fst of 0.057 and 0.042 for Sb and Sc, respectively). Cluster 1 was predominantly composed of pure Sb parasites and cluster 2 was mainly composed of pure Sc parasites, from Bamako and Kayes, respectively. Our study shows that cattle schistosomiasis remains endemic in Mali with S. bovis and S. curassoni. A robust genetic structure between the different schistosome populations was identified, which included two clusters based on the geographical distribution of the parasites.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"31 ","pages":"36"},"PeriodicalIF":2.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11218738/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141492976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prevalence and assemblage identified of Giardia duodenalis in zoo and farmed Asiatic black bears (Ursus thibetanus) from the Heilongjiang and Fujian Provinces of China. 中国黑龙江省和福建省动物园和养殖的亚洲黑熊（Ursus thibetanus）中十二指肠贾第鞭毛虫的流行率和聚集鉴定。

IF 2.3 2区医学

Parasite Pub Date : 2024-01-01 Epub Date: 2024-08-29 DOI: 10.1051/parasite/2024048

Jiani Chen, Liyuting Zhou, Wenjie Cao, Junchen Xu, Kuai Yu, Ting Zhang, Yiqing Wang, Jiayan Wang, Huicong Huang, Wei Zhao

{"title":"Prevalence and assemblage identified of Giardia duodenalis in zoo and farmed Asiatic black bears (Ursus thibetanus) from the Heilongjiang and Fujian Provinces of China.","authors":"Jiani Chen, Liyuting Zhou, Wenjie Cao, Junchen Xu, Kuai Yu, Ting Zhang, Yiqing Wang, Jiayan Wang, Huicong Huang, Wei Zhao","doi":"10.1051/parasite/2024048","DOIUrl":"10.1051/parasite/2024048","url":null,"abstract":"Captive and free-living wildlife serve as significant hosts for Giardia duodenalis. Asiatic black bears, valued for their economic and medicinal importance, are extensively farmed in China and also prevalent in zoos. However, studies on G. duodenalis in these animals in China are limited. Here, 218 feces samples of Asiatic black bears were collected: 36 from a zoo in Heilongjiang Province, and 182 from a farm in Fujian Province. Nested PCR of the SSU rRNA gene, followed by sequencing, was employed to determine the frequency and assemblage distribution of G. duodenalis. Positive samples underwent further analysis through multilocus genotyping (MLG) by amplifying the genes for glutamate dehydrogenase (gdh), β-giardin (bg), and triosephosphate isomerase (tpi). Of the 218 samples, G. duodenalis was detected in 22 cases at the SSU rRNA gene locus, including three from Heilongjiang and 19 from Fujian. Three assemblages were identified: A (n = 1), B (n = 16), and E (n = 2) in Fujian; and B (n = 3) in Heilongjiang. Out of the 22 positive samples, 20, 19, and 9 were effectively amplified and sequenced across the tpi, gdh, and bg loci, respectively. Seven samples were genotyped successfully at all three loci, identifying MLG-B1 (n = 1), MLG-B2 (n = 1), and MLG-B3 (n = 1), MLG-B4 (n = 1), MLG-B5 (n = 2), and MLG-B6 (n = 1) as the six assemblage B MLGs. This study marks the first documentation of G. duodenalis in Asiatic black bears in captivity in Fujian and Heilongjiang. The identification of zoonotic assemblages A and B, along with E, underscores potential public health concerns.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"31 ","pages":"50"},"PeriodicalIF":2.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11363899/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142110553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Occurrence and genetic characterization of Enterocytozoon bieneusi in pet dogs in Yunnan Province, China. 中国云南省宠物狗肠虫病的发生和遗传特征。

IF 2.9 2区医学

Parasite Pub Date : 2024-01-01 Epub Date: 2024-05-24 DOI: 10.1051/parasite/2024025

Jinhua Jian, Jinrong Zi, Yaxue Wang, Yaming Yang, Yaxing Su, Lan Yao, Benfu Li, Xiaoxue Peng, Jianping Cao, Yujuan Shen, Aiqin Liu

{"title":"Occurrence and genetic characterization of Enterocytozoon bieneusi in pet dogs in Yunnan Province, China.","authors":"Jinhua Jian, Jinrong Zi, Yaxue Wang, Yaming Yang, Yaxing Su, Lan Yao, Benfu Li, Xiaoxue Peng, Jianping Cao, Yujuan Shen, Aiqin Liu","doi":"10.1051/parasite/2024025","DOIUrl":"10.1051/parasite/2024025","url":null,"abstract":"Enterocytozoon bieneusi is the most common microsporidian species in humans and can affect over 200 animal species. Considering possible increasing risk of human E. bieneusi infection due to close contact with pet dogs and identification of zoonotic E. bieneusi genotypes, 589 fresh fecal specimens of pet dogs were collected from Yunnan Province, China to determine the occurrence of E. bieneusi, characterize dog-derived E. bieneusi isolates, and assess their zoonotic potential at the genotype level. Enterocytozoon bieneusi was identified and genotyped by PCR and sequencing of the internal transcribed spacer (ITS) region of the ribosomal RNA (rRNA) gene. Twenty-nine specimens (4.9%) were positive. A statistical difference was observed in occurrence rates of E. bieneusi in pet dogs among 11 sampling sites by Fisher's exact test. Fifteen genotypes were identified and all of them phylogenetically belonged to zoonotic group 1, including four known genotypes (EbpC, D, Peru 8, and Henan-III) and 11 novel genotypes. Genotype Henan-III was reported in dogs for the first time. The finding of known genotypes found previously in humans and novel genotypes falling into zoonotic group 1 indicates that dogs may play a role in the transmission of E. bieneusi to humans in the investigated areas.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"31 ","pages":"27"},"PeriodicalIF":2.9,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11120511/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141088281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diversity of Babesia spp. in skunks from selected states in the United States of America. 美国部分州臭鼬中巴贝斯菌属的多样性。

IF 2.3 2区医学

Parasite Pub Date : 2024-01-01 Epub Date: 2024-07-24 DOI: 10.1051/parasite/2024043

Kayla B Garrett, Justin Brown, Mourad Gabriel, Robert Dowler, J Clint Perkins, Dianna Krejsa, Michael J Yabsley

{"title":"Diversity of Babesia spp. in skunks from selected states in the United States of America.","authors":"Kayla B Garrett, Justin Brown, Mourad Gabriel, Robert Dowler, J Clint Perkins, Dianna Krejsa, Michael J Yabsley","doi":"10.1051/parasite/2024043","DOIUrl":"10.1051/parasite/2024043","url":null,"abstract":"Babesia species are intraerythrocytic protozoan parasites that infect a variety of hosts. The goal of this study was to evaluate the piroplasm species present in skunks in various states in the United States and determine whether there was any geographic variation. Spleen, whole blood, or blood on filter paper were received from Pennsylvania, Kentucky, North Carolina, South Carolina, Georgia, Missouri, Louisiana, Texas, Kansas, and California, and were tested for Babesia sp. We tested four species of skunks including striped skunk (Mephitis mephitis, n = 72), eastern spotted skunk (Spilogale putorius, n = 28), western spotted skunk (Spilogale gracilis, n = 15), and hog-nosed skunk (Conepatus leuconotus, n = 11). A PCR assay targeting the 18S rRNA region and cox1 region were used to determine if skunks were infected with piroplasms and for phylogenetic analyses. A total of 48.4% (61/126) of skunks tested positive for a Babesia species. Both the 18S and cox1 analysis supported a skunk-specific Babesia microti-like sp. of carnivores as well as a species in the B. microti complex that is phylogenetically unique from both B. microti of humans and the B. microti-like sp. of carnivores. In the 18S analysis, there was a third species of Babesia in hog-nosed skunks in the western piroplasm group. This study shows that at least three species of piroplasms occur in skunk species in the United States and further highlights the importance of phylogenetic analyses and the use of multiple gene targets when studying piroplasms.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"31 ","pages":"42"},"PeriodicalIF":2.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11271706/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141760255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prevalence of intestinal trichomonads in captive non-human primates in China. 中国圈养非人灵长类动物肠道滴虫的流行情况。

IF 2.9 2区医学

Parasite Pub Date : 2024-01-01 Epub Date: 2024-03-26 DOI: 10.1051/parasite/2024018

Ping-Ping Ma, Yang Zou, Wen-Jie Mu, Yue-Yue Zhang, Ya-Qi Li, Zhong-Li Liu, Long Zhang, Li-Xian Chen, Guo-Hua Liu, Shuai Wang

{"title":"Prevalence of intestinal trichomonads in captive non-human primates in China.","authors":"Ping-Ping Ma, Yang Zou, Wen-Jie Mu, Yue-Yue Zhang, Ya-Qi Li, Zhong-Li Liu, Long Zhang, Li-Xian Chen, Guo-Hua Liu, Shuai Wang","doi":"10.1051/parasite/2024018","DOIUrl":"10.1051/parasite/2024018","url":null,"abstract":"Trichomonads are protozoan symbionts with the capacity to infect vertebrates including humans and non-human primates (NHPs), sometimes with pathogenic effects. However, their diversity and prevalence in NHPs in China are poorly understood. A total of 533 fecal samples were collected from captive NHPs in Yunnan Province, China, of which 461 samples from Macaca fascicularis and 72 from Macaca mulatta. Trichomonadidae species were identified using PCR amplification of the ITS-1/5.8S/ITS-2 sequences. The overall prevalence of trichomonads in NHPs was determined to be 11.4% (61/533), with gender, diarrhea, and region identified as potential risk factors for the infections. Sequence alignment and phylogenetic analysis identified three species of trichomonads, i.e., Trichomitopsis minor (n = 45), Pentatrichomonas hominis (n = 11), and Tetratrichomonas sp. (n = 5). To the best of our knowledge, this is the first study to report Trichomitopsis minor infection in NHPs in China. Of note, Pentatrichomonas hominis is generally recognized as a parasitic organism affecting humans. Collectively, our results suggest that NHPs are potential sources of zoonotic trichomonad infections, highlighting the importance of surveillance and control measures to protect human and animal populations.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"31 ","pages":"19"},"PeriodicalIF":2.9,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10964852/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140288714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proliferation of MDSCs may indicate a lower ^CD4+ T cell immune response in schistosomiasis japonica. MDSCs 的增殖可能表明日本血吸虫病的 CD4+ T 细胞免疫反应较低。

IF 2.3 2区医学

Parasite Pub Date : 2024-01-01 Epub Date: 2024-08-29 DOI: 10.1051/parasite/2024050

Bo Peng, Yulin Luo, Shudong Xie, Quan Zhuang, Junhui Li, Pengpeng Zhang, Kai Liu, Yu Zhang, Chen Zhou, Chen Guo, Zhaoqin Zhou, Jie Zhou, Yu Cai, Meng Xia, Ke Cheng, Yingzi Ming

{"title":"Proliferation of MDSCs may indicate a lower CD4+ T cell immune response in schistosomiasis japonica.","authors":"Bo Peng, Yulin Luo, Shudong Xie, Quan Zhuang, Junhui Li, Pengpeng Zhang, Kai Liu, Yu Zhang, Chen Zhou, Chen Guo, Zhaoqin Zhou, Jie Zhou, Yu Cai, Meng Xia, Ke Cheng, Yingzi Ming","doi":"10.1051/parasite/2024050","DOIUrl":"10.1051/parasite/2024050","url":null,"abstract":"Background: Schistosoma japonicum (S. japonicum) is the main species of Schistosoma prevalent in China. Myeloid-derived suppressor cells (MDSCs) are important immunoregulatory cells and generally expand in parasite infection, but there is little research relating to MDSCs in Schistosoma infection.Methods: Fifty-six S. japonicum-infected patients were included in this study. MDSCs and percentages and absolute cell numbers of lymphocyte subsets, including CD3+ T cells, CD4+ T cells, CD8+ T cells, B cells and natural killer (NK) cells were detected using flow cytometry. The degree of liver fibrosis was determined using color Doppler ultrasound.Results: Patients infected with S. japonicum had a much higher percentage of MDSCs among peripheral blood mononuclear cells (PBMCs) than the healthy control. Regarding subpopulations of MDSCs, the percentage of granulocytic myeloid-derived suppressor cells (G-MDSCs) was clearly increased. Correlation analysis showed that the absolute cell counts of T-cell subsets correlated negatively with the percentages of MDSCs and G-MDSCs among PBMCs. The percentage of G-MDSCs in PBMCs was also significantly higher in patients with liver fibrosis diagnosed by color doppler ultrasound (grade > 0), and the percentage of G-MDSCs in PBMCs and liver fibrosis grading based on ultrasound showed a positive correlation.Conclusion: S. japonicum infection contributes to an increase in MDSCs, especially G-MDSCs, whose proliferation may inhibit the number of CD4+ T cells in peripheral blood. Meanwhile, there is a close relationship between proliferation of G-MDSCs and liver fibrosis in S. japonicum-infected patients.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"31 ","pages":"52"},"PeriodicalIF":2.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11363901/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142110554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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