Parasite最新文献_第6页

Relationship between acaricide resistance and acetylcholinesterase gene polymorphisms in the cattle tick Rhipicephalus microplus. 牛蜱 Rhipicephalus microplus 的杀螨剂抗性与乙酰胆碱酯酶基因多态性之间的关系。

IF 2.9 2区医学

Parasite Pub Date : 2024-01-01 Epub Date: 2024-02-04 DOI: 10.1051/parasite/2024003

Raquel Cossio-Bayugar, Francisco Martinez-Ibañez, Hugo Aguilar-Diaz, Estefan Miranda-Miranda

{"title":"Relationship between acaricide resistance and acetylcholinesterase gene polymorphisms in the cattle tick Rhipicephalus microplus.","authors":"Raquel Cossio-Bayugar, Francisco Martinez-Ibañez, Hugo Aguilar-Diaz, Estefan Miranda-Miranda","doi":"10.1051/parasite/2024003","DOIUrl":"10.1051/parasite/2024003","url":null,"abstract":"In this study, we aimed to develop a comprehensive methodology for identifying amino acid polymorphisms in acetylcholinesterase transcript 2 (AChE2) in acaricide-resistant Rhipicephalus microplus ticks. This included assessing AChE2 expression levels through qPCR and conducting 3D modeling to evaluate the interaction between acaricides and AChE2 using docking techniques. The study produced significant results, demonstrating that acaricide-resistant R. microplus ticks exhibit significantly higher levels of AChE expression than susceptible reference ticks. In terms of amino acid sequence, we identified 9 radical amino acid substitutions in AChE2 from acaricide-resistant ticks, when compared to the gene sequence of the susceptible reference strain. To further understand the implications of these substitutions, we utilized 3D acaricide-AChE2 docking modeling to examine the interaction between the acaricide and the AChE2 catalytic site. Our models suggest that these amino acid polymorphisms alter the configuration of the binding pocket, thereby contributing to differences in acaricide interactions and ultimately providing insights into the acaricide-resistance phenomenon in R. microplus.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10840460/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139692588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Schistosoma antigens: A future clinical magic bullet for autoimmune diseases? 血吸虫抗原：治疗自身免疫性疾病的未来临床灵丹妙药？

IF 2.3 2区医学

Parasite Pub Date : 2024-01-01 Epub Date: 2024-10-31 DOI: 10.1051/parasite/2024067

Mphatso Mayuni Chaponda, Ho Yin Pekkle Lam

{"title":"Schistosoma antigens: A future clinical magic bullet for autoimmune diseases?","authors":"Mphatso Mayuni Chaponda, Ho Yin Pekkle Lam","doi":"10.1051/parasite/2024067","DOIUrl":"10.1051/parasite/2024067","url":null,"abstract":"Autoimmune diseases are characterized by dysregulated immunity against self-antigens. Current treatment of autoimmune diseases largely relies on suppressing host immunity to prevent excessive inflammation. Other immunotherapy options, such as cytokine or cell-targeted therapies, have also been used. However, most patients do not benefit from these therapies as recurrence of the disease usually occurs. Therefore, more effort is needed to find alternative immune therapeutics. Schistosoma infection has been a significant public health problem in most developing countries. Schistosoma parasites produce eggs that continuously secrete soluble egg antigen (SEA), which is a known modulator of host immune responses by enhancing Th2 immunity and alleviating outcomes of Th1 and Th17 responses. Recently, SEA has shown promise in treating autoimmune disorders due to their substantial immune-regulatory effects. Despite this interest, how these antigens modulate human immunity demonstrates only limited pieces of evidence, and whether there is potential for Schistosoma antigens in other diseases in the future remains an unsolved question. This review discusses how SEA modulates human immune responses and its potential for development as a novel immunotherapeutic for autoimmune diseases. We also discuss the immune modulatory effects of other non-SEA schistosome antigens at different stages of the parasite's life cycle.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11527426/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142558430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phlebotomine sand fly distribution and abundance in France: A systematic review. 沙蝇在法国的分布和数量：系统回顾。

IF 2.3 2区医学

Parasite Pub Date : 2024-01-01 Epub Date: 2024-08-07 DOI: 10.1051/parasite/2024045

Jorian Prudhomme, Jérôme Depaquit, Florence Robert-Gangneux

{"title":"Phlebotomine sand fly distribution and abundance in France: A systematic review.","authors":"Jorian Prudhomme, Jérôme Depaquit, Florence Robert-Gangneux","doi":"10.1051/parasite/2024045","DOIUrl":"10.1051/parasite/2024045","url":null,"abstract":"Global changes in climate are contributing to modified Phlebotomine sand fly presence and activity, and the distribution of the pathogens they transmit (e.g., Leishmania and Phlebovirus), and are leading to their possible extension toward northern France. To predict the evolution of these pathogens and control their spread, it is essential to identify and characterize the presence and abundance of potential vectors. However, there are no recent publications describing sand fly species distribution in France. Consequently, we carried out a systematic review to provide distribution and abundance maps over time, along with a simplified dichotomous key for species in France. The review adhered to PRISMA guidelines, resulting in 172 relevant capture reports from 168 studies out of the 2646 documents retrieved, of which 552 were read and 228 analyzed. Seven species were recorded and categorized into three groups based on their abundance: low abundance species, abundant but little-studied species, and abundant vector species. Sand flies are certainly present throughout France but there is a greater diversity of species in the Mediterranean region. Phlebotomus perniciosus and Ph. ariasi are the most abundant and widely distributed species, playing a role as vectors of Leishmania. Sergentomyia minuta, though very abundant, remains under-studied, highlighting the need for further research. Phlebotomus papatasi, Ph. perfiliewi, Ph. sergenti, and Ph. mascittii are present in low numbers and are less documented, limiting understanding of their potential role as vectors. This work provides the necessary basis for comparison of field data generated in the future.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11305120/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141897960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphology, complete mitochondrial genome, and molecular phylogeny of Rhabdias macrocephalum n. sp. (Nematoda: Rhabdiasidae) from Diploderma splendidum (Reptilia: Agamidae). 来自Diploderma splendidum (Reptilia: Agamidae) 的Rhabdias macrocephalum n. sp.（线虫纲：Rhabdiasidae）的形态学、完整线粒体基因组和分子系统发育。

IF 2.3 2区医学

Parasite Pub Date : 2024-01-01 Epub Date: 2024-08-14 DOI: 10.1051/parasite/2024046

Jia-Lu Zeng, Hui-Xia Chen, Hong-Ru Xu, Liang Li

{"title":"Morphology, complete mitochondrial genome, and molecular phylogeny of Rhabdias macrocephalum n. sp. (Nematoda: Rhabdiasidae) from Diploderma splendidum (Reptilia: Agamidae).","authors":"Jia-Lu Zeng, Hui-Xia Chen, Hong-Ru Xu, Liang Li","doi":"10.1051/parasite/2024046","DOIUrl":"10.1051/parasite/2024046","url":null,"abstract":"Species of the genus Rhabdias Stiles & Hassall, 1905 are common parasitic nematodes occurring in the lungs of amphibians and reptiles worldwide. In the present study, Rhabdias macrocephalum n. sp. is described using integrated morphological methods (light and scanning electron microscopy) and molecular approaches (sequencing of the nuclear 28S and ITS regions, and mitochondrial cox1, cox2, and 12S genes) based on specimens collected from the green striped tree dragon Diploderma splendidum (Barbour & Dunn) (Reptilia: Agamidae) in China. The complete mitochondrial genome of R. macrocephalum n. sp. was sequenced and annotated: it is 14,819 bp in length, including 12 protein coding genes (missing atp8), 22 tRNA genes, 2 rRNA genes and three non-coding regions. The gene arrangement of R. macrocephalum n. sp. is different from all of the currently available mitogenomes of nematodes and represents a novel type of mitochondrial gene arrangement reported in Nematoda. Molecular phylogenetic results based on the ITS + 28S data support the monophyly of Entomelas, Pneumonema, Serpentirhabdias, and Rhabdias, and showed R. macrocephalum n. sp. forming a most basal lineage in Rhabdias.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11323812/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141976307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficacy of a combination of esafoxolaner, eprinomectin and praziquantel (NexGard^® Combo) against Thelazia callipaeda in naturally infected cats. 伊沙福柳胺、伊普拉菌素和吡喹酮复方制剂（NexGard® Combo）对自然感染猫科动物中的卡氏疟原虫（Thelazia callipaeda）的疗效。

IF 2.9 2区医学

Parasite Pub Date : 2024-01-01 Epub Date: 2024-02-28 DOI: 10.1051/parasite/2024008

Angela Di Cesare, Stefania Zanet, Donato Traversa, Mariasole Colombo, Eric Tielemans, Frederic Beugnet, Ezio Ferroglio

{"title":"Efficacy of a combination of esafoxolaner, eprinomectin and praziquantel (NexGard® Combo) against Thelazia callipaeda in naturally infected cats.","authors":"Angela Di Cesare, Stefania Zanet, Donato Traversa, Mariasole Colombo, Eric Tielemans, Frederic Beugnet, Ezio Ferroglio","doi":"10.1051/parasite/2024008","DOIUrl":"10.1051/parasite/2024008","url":null,"abstract":"This clinical study assessed the efficacy of a topical combination of esafoxolaner, eprinomectin and praziquantel (NexGard® Combo) in treating cats naturally infected with the eyeworm Thelazia callipaeda (Nematoda, Thelaziidae). On Study Day (SD) 0, sixteen client-owned cats with eyeworm infection were allocated to an untreated control group (G1, 8 cats) or to a NexGard® Combo treated group (G2, 8 cats) and subjected to ocular examination. Cats in G2 received the treatment as per label recommendations. On SD 7 and 14 (±1), cats were examined for the presence of eyeworms and clinical signs. On SD 14, eyeworms were collected and counted. On SD 7 and 14, all cats in G1 were still infected with eyeworms, while G2 cats were free from eyeworms on SD 7 and 14, demonstrating 100% efficacy (p < 0.0001). All collected eyeworms were morphologically and molecularly confirmed to be T. callipaeda. On SD 0, fifteen out of the sixteen cats (7 in G1 and 8 in G2) displayed inflammatory ocular signs. On SD 7, all eight untreated cats and seven treated cats displayed inflammatory ocular signs. On SD 14, five out of eight G2 treated cats had recovered, while the eight untreated cats still displayed inflammatory ocular signs. The treatment significantly reduced lacrimation and conjunctivitis (p = 0.0001). No adverse reactions occurred. This clinical study provides evidence that NexGard® Combo is highly safe and effective for the treatment of T. callipaeda infection in cats under field conditions.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10901074/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139983459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sterile Insect Technique (SIT) field trial targeting the suppression of Aedes albopictus in Greece. 以抑制希腊白纹伊蚊为目标的昆虫不育技术（SIT）实地试验。

IF 2.9 2区医学

Parasite Pub Date : 2024-01-01 Epub Date: 2024-03-26 DOI: 10.1051/parasite/2024020

Georgios Balatsos, Vasileios Karras, Arianna Puggioli, Fabrizio Balestrino, Romeo Bellini, Dimitrios P Papachristos, Panagiotis G Milonas, Nikos T Papadopoulos, Marco Malfacini, Marco Carrieri, Apostolos Kapranas, Wadaka Mamai, George Mastronikolos, Ioanna Lytra, Jérémy Bouyer, Antonios Michaelakis

{"title":"Sterile Insect Technique (SIT) field trial targeting the suppression of Aedes albopictus in Greece.","authors":"Georgios Balatsos, Vasileios Karras, Arianna Puggioli, Fabrizio Balestrino, Romeo Bellini, Dimitrios P Papachristos, Panagiotis G Milonas, Nikos T Papadopoulos, Marco Malfacini, Marco Carrieri, Apostolos Kapranas, Wadaka Mamai, George Mastronikolos, Ioanna Lytra, Jérémy Bouyer, Antonios Michaelakis","doi":"10.1051/parasite/2024020","DOIUrl":"10.1051/parasite/2024020","url":null,"abstract":"The sterile insect technique (SIT) involves releasing large numbers of sterile males to outcompete wild males in mating with females, leading to a decline in pest populations. In the current study, we conducted a suppression trial in Greece against the invasive dengue vector mosquito Aedes albopictus (Skuse) through the weekly release of sterile males for 22 weeks from June to September 2019. Our approach included the long-distance transport of sterile mosquitoes, and their release at a density of 2,547 ± 159 sterile males per hectare per week as part of an area-wide integrated pest management strategy (AW-IPM). The repeated releases of sterile males resulted in a gradual reduction in egg density, reaching 78% from mid-June to early September. This reduction remained between 70% and 78% for four weeks after the end of the releases. Additionally, in the SIT intervention area, the ovitrap index, representing the percentage of traps containing eggs, remained lower throughout the trial than in the control area. This trial represents a significant advance in the field of mosquito control, as it explores the viability and efficacy of producing and transporting sterile males from a distant facility to the release area. Our results provide valuable insights for future SIT programmes targeting Ae. Albopictus, and the methodology we employed can serve as a starting point for developing more refined and effective release protocols, including the transportation of sterile males over long distances from production units to intervention areas.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10964849/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140288715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel phylogenetic clade of avian Haemoproteus parasites (Haemosporida, Haemoproteidae) from Accipitridae raptors, with description of a new Haemoproteus species. 来自翼手目猛禽的新的寄生虫系统发育支系（血孢子虫科，血蛋白虫科），并描述了一个新的血蛋白虫物种。

IF 2.9 2区医学

Parasite Pub Date : 2024-01-01 Epub Date: 2024-02-08 DOI: 10.1051/parasite/2023066

Josef Harl, Anaïs Fauchois, Marie-Pierre Puech, Delphine Gey, Frédéric Ariey, Brigitte Izac, Herbert Weissenböck, Nayden Chakarov, Tatjana Iezhova, Gediminas Valkiūnas, Linda Duval

{"title":"Novel phylogenetic clade of avian Haemoproteus parasites (Haemosporida, Haemoproteidae) from Accipitridae raptors, with description of a new Haemoproteus species.","authors":"Josef Harl, Anaïs Fauchois, Marie-Pierre Puech, Delphine Gey, Frédéric Ariey, Brigitte Izac, Herbert Weissenböck, Nayden Chakarov, Tatjana Iezhova, Gediminas Valkiūnas, Linda Duval","doi":"10.1051/parasite/2023066","DOIUrl":"10.1051/parasite/2023066","url":null,"abstract":"Avian haemosporidian parasites (order Haemosporida, phylum Apicomplexa) are blood and tissue parasites transmitted by blood-sucking dipteran insects. Three genera (Plasmodium, Haemoproteus and Leucocytozoon) have been most often found in birds, with over 270 species described and named in avian hosts based mainly on the morphological characters of blood stages. A broad diversity of Haemoproteus parasites remains to be identified and characterized morphologically and molecularly, especially those infecting birds of prey, an underrepresented bird group in haemosporidian parasite studies. The aim of this study was to investigate and identify Haemoproteus parasites from a large sample comprising accipitriform raptors of 16 species combining morphological and new molecular protocols targeting the cytb genes of this parasite group. This study provides morphological descriptions and molecular characterizations of two Haemoproteus species, H. multivacuolatus n. sp. and H. nisi Peirce and Marquiss, 1983. Haemoproteus parasites of this group were so far found in accipitriform raptors only and might be classified into a separate subgenus or even genus. Cytb sequences of these parasites diverge by more than 15% from those of all others known avian haemosporidian genera and form a unique phylogenetic clade. This study underlines the importance of developing new diagnostic tools to detect molecularly highly divergent parasites that might be undetectable by commonly used conventional tools.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10854483/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139707509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intraspecific variation in Gyrodactylus mediotorus and G. crysoleucas (Gyrodactylidae) from Nearctic shiners (Leuciscidae): evidence for ongoing speciation, host-switching, and parasite translocation. 来自近北极胫鱼（Leuciscidae）的Gyrodactylus mediotorus和G. crysoleucas（Gyrodactylidae）的种内变异：正在进行的物种分化、宿主转换和寄生虫迁移的证据。

IF 2.9 2区医学

Parasite Pub Date : 2024-01-01 Epub Date: 2024-06-11 DOI: 10.1051/parasite/2024023

Chahrazed Rahmouni, Mária Seifertová, Megan G Bean, Andrea Šimková

{"title":"Intraspecific variation in Gyrodactylus mediotorus and G. crysoleucas (Gyrodactylidae) from Nearctic shiners (Leuciscidae): evidence for ongoing speciation, host-switching, and parasite translocation.","authors":"Chahrazed Rahmouni, Mária Seifertová, Megan G Bean, Andrea Šimková","doi":"10.1051/parasite/2024023","DOIUrl":"10.1051/parasite/2024023","url":null,"abstract":"A parasitological investigation of Cyprinella venusta and Notropis cf. stramineus sampled in Texas, USA, in the Guadalupe River, revealed the presence of Gyrodactylus crysoleucas Mizelle and Kritsky, 1967 on C. venusta, and Gyrodactylus mediotorus King, Marcogliese, Forest, McLaughlin & Bentzen, 2013 on both fish species. This represents new leuscicid fish hosts and locality records for these two gyrodactylids. Gyrodactylus crysoleucas previously identified from both non-native Californian Notemigonus crysoleucas and from farmed stocks in Minnesota demonstrated intraspecific variability in terms of morphology and genetics as a local adaptation associated with isolation by distance. Results further confirmed G. crysoleucas as alien in the western USA and suggested host-switching involving C. venusta and N. crysoleucas. Conservative morphology and genetics on the part of G. mediotorus from C. venusta and N. cf. stramineus (Guadalupe River) was observed, while higher genetic divergence in the ITS sequences associated with morphological discrepancy was found between the studied G. mediotorus specimens and those of Notropis hudsonius than when considering the parasites of Notropis texanus. The separation of G. mediotorus into geographical subgroups may indicate ongoing speciation linked to the Pleistocene glaciations in North America, and to hydrographic barriers that facilitated separate evolutionary paths leading to speciation. We suggest that deep investigations of Gyrodactylus populations will help to understand the speciation of these parasites and their adaptation to Nearctic fish hosts.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11166112/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141301205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptome changes of liver fluke Opisthorchis viverrini in diabetic hamsters. 糖尿病仓鼠肝吸虫Opisthorchis viverrini的转录组变化

IF 2.3 2区医学

Parasite Pub Date : 2024-01-01 Epub Date: 2024-09-13 DOI: 10.1051/parasite/2024056

Apisit Chaidee, Naruechar Charoenram, Chatchawan Sengthong, Rungtiwa Dangtakot, Porntip Pinlaor, Thatsanapong Pongking, Somchai Pinlaor

{"title":"Transcriptome changes of liver fluke Opisthorchis viverrini in diabetic hamsters.","authors":"Apisit Chaidee, Naruechar Charoenram, Chatchawan Sengthong, Rungtiwa Dangtakot, Porntip Pinlaor, Thatsanapong Pongking, Somchai Pinlaor","doi":"10.1051/parasite/2024056","DOIUrl":"https://doi.org/10.1051/parasite/2024056","url":null,"abstract":"A recent study in hamsters showed that infection with the liver fluke Opisthorchis viverrini in diabetic hosts worsens the severity of hepatobiliary disease. However, the effects of diabetes on the worm's phenotype and gene expression pattern remain unknown. This study investigated the impact of diabetes on the global gene expression and development of O. viverrini in diabetic hamsters. Parasitological parameters were assessed, and mRNA sequencing with bioinformatic analysis was performed. The study revealed that worm establishment rates in diabetic hamsters were directly correlated with fasting plasma glucose levels. Interestingly, worms collected from diabetic hosts exhibited stunted growth and reduced egg production. Transcriptomic analysis revealed significant alterations in gene expression, with 4314 and 567 differentially expressed genes at 21- and 35-days post-infection, respectively. Gene ontology enrichment analysis highlighted changes in biological processes related to stress response, metabolism, and cellular organization. Notably, genes associated with parasite virulence, including granulin, tetraspanins, and thioredoxins, showed significant upregulation in diabetic hosts. These findings demonstrate the profound impact of host diabetic status on O. viverrini development and gene expression, providing insights into the complex interplay between host metabolism and parasite biology, including molecular adaptations of O. viverrini in hosts. This study contributes to our understanding of opisthorchiasis in the context of metabolic disorders and may inform future strategies for disease management in diabetic human populations.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11396942/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142292914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gasterophilus intestinalis infestation in lion (Panthera leo) and plains zebra (Equus quagga) in the Serengeti ecosystem: Morphological and molecular profiling. 塞伦盖蒂生态系统中狮子（Panthera leo）和平原斑马（Equus quagga）的肠道Gasterophilus虫害：形态学和分子分析。

IF 2.3 2区医学

Parasite Pub Date : 2024-01-01 Epub Date: 2024-09-27 DOI: 10.1051/parasite/2024060

Barakaeli Abdieli Ndossi, Eblate Ernest Mjingo, Maulid Mzinga Mdaki, Marry Wokusima Zebedayo, Seongjun Choe, Mohammed Mebarek Bia, Heejae Yang, Sungbo Seo, Keeseon S Eom

{"title":"Gasterophilus intestinalis infestation in lion (Panthera leo) and plains zebra (Equus quagga) in the Serengeti ecosystem: Morphological and molecular profiling.","authors":"Barakaeli Abdieli Ndossi, Eblate Ernest Mjingo, Maulid Mzinga Mdaki, Marry Wokusima Zebedayo, Seongjun Choe, Mohammed Mebarek Bia, Heejae Yang, Sungbo Seo, Keeseon S Eom","doi":"10.1051/parasite/2024060","DOIUrl":"https://doi.org/10.1051/parasite/2024060","url":null,"abstract":"This study was conducted to clarify the host specificity and the geographical distribution of Gasterophilus species (Diptera, Oestridae) in the Serengeti ecosystem. A total of 317 larvae were recovered from two common zebras (Equus quagga, formerly Equus burchellii) in Maswa Game Reserve, and 58 larvae were recovered from an African lion (Panthera leo) in the Serengeti National Park. The study emphasizes the rare occurrence of Gasterophilus sp. in lions, shedding light on the broader life cycle and physiological implications for hosts. Genetic analysis of cox2 genes from Gasterophilus species, sourced from a single geographic location, reveals significant genetic distinctions and host specificity. This study reports the first case of G. intestinalis infestation in an African lion in the Serengeti ecosystem, extending its known range from zebras and other equids, and highlighting ecological and veterinary implications. This unusual prey-predator transmission highlights the value of molecular taxonomic tools in clarifying host-parasite dynamics and guiding targeted conservation strategies.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11433831/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142351527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0