Parasite最新文献_第6页

Leishmaniases: Strategies in treatment development. 利什曼病：治疗发展策略。

IF 2.3 2区医学

Parasite Pub Date : 2025-01-01 Epub Date: 2025-03-05 DOI: 10.1051/parasite/2025009

Alissa Majoor, Grégory Michel, Pierre Marty, Laurent Boyer, Christelle Pomares

{"title":"Leishmaniases: Strategies in treatment development.","authors":"Alissa Majoor, Grégory Michel, Pierre Marty, Laurent Boyer, Christelle Pomares","doi":"10.1051/parasite/2025009","DOIUrl":"10.1051/parasite/2025009","url":null,"abstract":"Leishmaniases are vector-borne parasitic diseases that pose a threat to over 1 billion people worldwide. The parasites target cells of the reticulohistiocytic system, such as macrophages, where they replicate. The disease manifests in various forms, ranging from localized cutaneous leishmaniasis to life-threatening visceral forms, which are fatal in 95% of cases without treatment. Current treatments rely on the invasive administration of toxic and expensive drugs that are increasingly encountering resistance. Therefore, finding alternative treatments for this disease is imperative. This literature review focuses on recent advancements in alternative treatments and aims to present the various strategies designed to address current limitations, including cost, toxicity, off-target effects, administration routes, and the emergence of drug resistance. Starting with an overview of the existing approved treatments and their specific limitations, we categorize treatment development strategies into five key sections: (i) combination therapies using existing approved treatments to enhance efficacy and reduce resistance; (ii) nanoparticle formulations, which enable targeted delivery to infected organs and improved therapeutic efficiency; (iii) drug repositioning, a strategy that has already contributed to the approval of over half of current therapeutic compounds; (iv) immunomodulation, used in conjunction with standard chemotherapies to enhance treatment efficacy and lower relapse rates; and (v) ethnobotanicals, which have demonstrated promising in vitro results by combining low toxicity, immunomodulatory properties, and potent anti-parasitic effects. In summary, this review outlines current strategies in treatment development, emphasizing their advantages over conventional therapies while acknowledging their limitations.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"32 ","pages":"18"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11882135/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143567843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Usefulness of microsatellite loci for differentiating between Dibothriocephalus dendriticus and Dibothriocephalus ditremus (Cestoda: Diphyllobothriidea). 微卫星位点在树状双胸头与双胸头鉴别中的应用。

IF 2.3 2区医学

Parasite Pub Date : 2025-01-01 Epub Date: 2025-07-04 DOI: 10.1051/parasite/2025033

Ivica Králová-Hromadová, Lucia Dinisová, Alžbeta Radačovská, Egil Karlsbakk, Karl Skírnisson, Eva Čisovská Bazsalovicsová

{"title":"Usefulness of microsatellite loci for differentiating between Dibothriocephalus dendriticus and Dibothriocephalus ditremus (Cestoda: Diphyllobothriidea).","authors":"Ivica Králová-Hromadová, Lucia Dinisová, Alžbeta Radačovská, Egil Karlsbakk, Karl Skírnisson, Eva Čisovská Bazsalovicsová","doi":"10.1051/parasite/2025033","DOIUrl":"10.1051/parasite/2025033","url":null,"abstract":"Differentiating between two diphyllobothriid tapeworms Dibothriocephalus dendriticus and Dibothriocephalus ditremus is complicated due to their morphological plasticity, intraspecific variability and a wide range of common hosts. The aim of this study was to develop a species-specific PCR-based method for single-step discrimination between D. dendriticus and D. ditremus. Intraspecific variation and interspecific differences were analysed in subunits/spacers of nuclear rRNA genes and protein-coding genes of mitochondrial DNA. In addition, the specificity of primers designed for the amplification of microsatellite loci in D. dendriticus was tested on D. ditremus DNA. Due to high identity within the rRNA gene in these species, no suitable DNA regions could be identified for the design of the species-specific primers. A higher level of interspecific differences was detected in the mitochondrial cox1 and cob genes, in which regions containing species-specific mutations were chosen for the design of D. dendriticus- and D. ditremus-specific primers. However, their specificity was not confirmed, as the D. dendriticus-specific primers also annealed to D. ditremus DNA and vice versa. Of the 15 primer pairs designed for the amplification of microsatellite loci in D. dendriticus, 13 primer pairs also annealed to D. ditremus DNA. Only two primer pairs, which amplify the Dd_8 and Dd_33 loci have been proven to be D. dendriticus-specific. The effectiveness and high reproducibility of the Dd_8 primers were validated on ~3,500 D. dendriticus and D. ditremus plerocercoids from Iceland and Norway. These primers are recommended for future molecular differentiation between both Dibothriocephalus species.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"32 ","pages":"41"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12232403/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144560738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular characterization and subtype analysis of Blastocystis sp. in captive wildlife in Henan, China. 河南圈养野生动物囊虫的分子特征及亚型分析。

IF 2.3 2区医学

Parasite Pub Date : 2025-01-01 Epub Date: 2025-02-17 DOI: 10.1051/parasite/2025006

Zhaohui Cui, Xiyao Huang, Sulan Zhang, Kaifang Li, Aili Zhang, Qichen Li, Yutong Zhang, Junqiang Li, Meng Qi

{"title":"Molecular characterization and subtype analysis of Blastocystis sp. in captive wildlife in Henan, China.","authors":"Zhaohui Cui, Xiyao Huang, Sulan Zhang, Kaifang Li, Aili Zhang, Qichen Li, Yutong Zhang, Junqiang Li, Meng Qi","doi":"10.1051/parasite/2025006","DOIUrl":"10.1051/parasite/2025006","url":null,"abstract":"Blastocystis sp. is a zoonotic intestinal protozoan that is ubiquitous globally, residing in the gastrointestinal tracts of both humans and various animals. In the present study, a PCR-sequencing tool based on the SSU rRNA gene was employed to investigate the prevalence and subtypes of Blastocystis spp. in 204 fresh fecal samples collected from 20 captive wildlife species from a bird park in Henan Province, Central China. Overall, Blastocystis was present in 13.73% (28 out of 204) of the samples and 25% (5 out of 20) of the species. A total of four zoonotic subtypes of Blastocystis sp. were found: ST1, ST3, ST5, and ST27, with the latter being the most prevalent, accounting for 35.71% (10 out of 28) of the 5 species positive for Blastocystis sp. To the best of our knowledge, this is the first report of Blastocystis ST27 in birds in China, namely bar-headed goose (Anser indicus) and peafowl (Pavo muticus). The data suggest that captive wildlife, particularly those in bird parks, may frequently be infected with this zoonotic pathogen. Consequently, these animals may serve as potential reservoirs for zoonotic infections in humans.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"32 ","pages":"11"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11832164/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143441250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphological and molecular characterization of Henneguya cystigena n. sp. (Cnidaria, Myxosporea) parasitizing the alimentary tract of yellowfin seabream, Acanthopagrus latus, in the East China Sea. 东海黄鳍海棘鱼（Acanthopagrus latus）消化道寄生线虫的形态与分子特征。

IF 2.4 2区医学

Parasite Pub Date : 2025-01-01 Epub Date: 2025-08-15 DOI: 10.1051/parasite/2025048

Bo Zhang, Fei Yin

{"title":"Morphological and molecular characterization of Henneguya cystigena n. sp. (Cnidaria, Myxosporea) parasitizing the alimentary tract of yellowfin seabream, Acanthopagrus latus, in the East China Sea.","authors":"Bo Zhang, Fei Yin","doi":"10.1051/parasite/2025048","DOIUrl":"10.1051/parasite/2025048","url":null,"abstract":"A novel myxosporean species was identified. The species formed spherical to ellipsoidal pseudocysts within the alimentary tract wall of a yellowfin seabream Acanthopagrus latus fished in the East China Sea. Histological examination confirmed that pseudocysts were localized within the submucosal layer of the stomach wall. Round to ellipsoidal myxospores exhibited two posterior caudal appendages, consistent with the morphological characteristics of the genus Henneguya. The myxospore body measured 9.6 ± 0.5 (8.6-10.6) μm in length, 7.3 ± 0.4 (6.8-7.9) μm in width, and 6.0 ± 0.2 (5.5-6.4) μm in thickness. Two equal pyriform polar capsules were observed, measuring 3.5 ± 0.3 (2.9-4.4) μm × 1.9 ± 0.2 (1.4-2.2) μm. Pairwise comparison referring to small subunit ribosomal DNA sequence revealed a highest identity of 94.19% with Henneguya yokoyamai Li et al., 2012, supporting the classification of the specimens as a new species, Henneguya cystigena n. sp. Phylogenetic analyses demonstrated intermixed groupings of myxobolid species, highlighting persistent discrepancies between traditional morphological taxonomy and increasingly refined molecular phylogeny. To the best of our knowledge, this study represents the first description of a Henneguya species parasitizing a marine fish in the East China Sea near mainland China.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"32 ","pages":"51"},"PeriodicalIF":2.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12355913/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144855982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Trichinella spiralis excretory-secretory antigens on expression of indoleamine 2, 3-dioxygenase on dendritic cells in vitro. 旋毛虫排泄分泌抗原对体外树突状细胞吲哚胺2,3 -双加氧酶表达的影响。

IF 2.3 2区医学

Parasite Pub Date : 2025-01-01 Epub Date: 2025-04-16 DOI: 10.1051/parasite/2025018

Wenhao Yu, Xuhong Yuan, Peng Zhai, Xiaoyun Li, Caixia Han

{"title":"Effects of Trichinella spiralis excretory-secretory antigens on expression of indoleamine 2, 3-dioxygenase on dendritic cells in vitro.","authors":"Wenhao Yu, Xuhong Yuan, Peng Zhai, Xiaoyun Li, Caixia Han","doi":"10.1051/parasite/2025018","DOIUrl":"https://doi.org/10.1051/parasite/2025018","url":null,"abstract":"Indoleamine 2, 3-dioxygenase (IDO) is a potent immunoenzyme found in dendritic cells (DCs). Research has demonstrated that Trichinella spiralis induces IDO expression in the host immune response through its excretory-secretory (ES) antigens. However, the role of IDO in the immune response to T. spiralis remains unclear. To examine the effects of T. spiralis ES antigens on IDO expression in DCs in vitro, assessments were conducted using qRT-PCR, Western blotting (WB), flow cytometry, and siRNA transfer. The findings indicated that ES antigen stimulation upregulated IDO expression in DCs in vitro. Furthermore, ES antigen significantly enhanced the expression of the proinflammatory cytokines TNF-α and IFN-γ, along with the anti-inflammatory cytokine IL-10, downstream of IDO in DCs. Flow cytometry analysis confirmed that surface molecules CD40, MHC-II, CD80, and CD86 on DCs were upregulated following stimulation with ES antigen and lipopolysaccharide (LPS). Compared to the ES antigen alone, siRNA620 effectively inhibited IDO levels, demonstrating a statistically significant reduction. Continuous stimulation of DCs by ES antigens may lead to immune tolerance through the activation of IDO-mediated inflammation-associated factors. These results suggest that IDO expression in DCs plays a crucial role in T. spiralis infection.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"32 ","pages":"26"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12002673/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144027762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Population structure of Clinostomum complanatum (Trematoda: Digenea) with new data on haplotype diversity of flukes from Slovakia and Italy. 斯洛伐克和意大利吸虫单倍型多样性的新数据及吸虫种群结构。

IF 2.3 2区医学

Parasite Pub Date : 2025-01-01 Epub Date: 2025-01-22 DOI: 10.1051/parasite/2024080

Ľudmila Juhásová, Eva Čisovská Bazsalovicsová, Monica Caffara, Alžbeta Radačovská, Andrea Gustinelli, Lucia Dinisová, Yaroslav Syrota, Ivica Králová-Hromadová

{"title":"Population structure of Clinostomum complanatum (Trematoda: Digenea) with new data on haplotype diversity of flukes from Slovakia and Italy.","authors":"Ľudmila Juhásová, Eva Čisovská Bazsalovicsová, Monica Caffara, Alžbeta Radačovská, Andrea Gustinelli, Lucia Dinisová, Yaroslav Syrota, Ivica Králová-Hromadová","doi":"10.1051/parasite/2024080","DOIUrl":"10.1051/parasite/2024080","url":null,"abstract":"The fluke Clinostomum complanatum, a parasite of piscivorous birds, but also reptiles and rarely mammals, has established several foci in the western Palaearctic regions. Previous studies pointed out the complicated taxonomy of the genus, but broader population genetic analysis of C. complanatum has not yet been carried out. The aim of this study was to determine the structure, intraspecific variability, and diversity of mitochondrial cox1 haplotypes of C. complanatum from different localities in Slovakia (Danube floodplain forests) and Italy (Emilia-Romagna and Tuscany), as well as to evaluate the interrelationships among populations from Europe, the Middle East, and North Africa. The genetic structure of C. complanatum from Slovakia and Italy was represented by a great number of haplotypes, showing stable populations with high intraspecific diversity. The haplotypes of samples from other localities (Romania, Turkey, Egypt, and Iran) showed possible gene flow among the populations from Central Europe down to the Mediterranean region, North Africa, and the Middle East. The genetic homogeneity of these samples can be linked to the distribution and migratory routes of the definitive hosts, aquatic piscivorous birds, mainly herons and cormorants, that spread parasite eggs among the continents.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"32 ","pages":"3"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11752738/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143009450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Discovery of new species of mesoparasitic pennellid (Copepoda: Siphonostomatoida) from the endemic mesopelagic lightfish Vinciguerria mabahiss in the Red Sea. 红海特有中远洋轻鱼中寄生盘尾虫新种的发现（桡足目：虹口虫）。

IF 2.3 2区医学

Parasite Pub Date : 2025-01-01 Epub Date: 2025-07-16 DOI: 10.1051/parasite/2025038

Kah Kheng Lim, Carlos Angulo-Preckler, Lotfi J Rabaoui, Mohammad A Qurban, Vincent A Pieribone, Carlos M Duarte, Daisuke Uyeno

{"title":"Discovery of new species of mesoparasitic pennellid (Copepoda: Siphonostomatoida) from the endemic mesopelagic lightfish Vinciguerria mabahiss in the Red Sea.","authors":"Kah Kheng Lim, Carlos Angulo-Preckler, Lotfi J Rabaoui, Mohammad A Qurban, Vincent A Pieribone, Carlos M Duarte, Daisuke Uyeno","doi":"10.1051/parasite/2025038","DOIUrl":"10.1051/parasite/2025038","url":null,"abstract":"A new species of the genus Cardiodectes Wilson, 1917 (Siphonostomatoida: Pennellidae), Cardiodectes tofaili n. sp., is described based on 13 adult females from ten specimens of the endemic lightfish Vinciguerria mabahiss (Stomiiformes: Phosichthyidae). These hosts were inadvertently captured by a remotely operated vehicle at depths of 454-645 m in the pelagic waters of the Saudi Arabian Red Sea. The new species is placed under the \"rubosus\" group, characterized by possession of a trunk without a discrete abdomen. It is distinguished from its 12 congeners within this group by having a short neck region with a distinct fourth pedigerous somite, and a trunk that is ca. 5 times longer than wide. Phylogenetic analysis based on concatenated 18S + 28S rDNA sequences supports the distinctiveness of the new species. This species is endemic to the Red Sea, representing the first recorded mesoparasite from the mesopelagic environment of the region. This discovery highlights the unique biodiversity of the Red Sea and underscores the importance of exploring mesopelagic ecosystems.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"32 ","pages":"43"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12266664/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144650055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correlation between imaging features and rEm18 antibodies in alveolar echinococcosis: results from a multicenter study in France. 肺泡包虫病的影像学特征与rEm18抗体的相关性：来自法国一项多中心研究的结果

IF 2.3 2区医学

Parasite Pub Date : 2025-01-01 Epub Date: 2025-02-07 DOI: 10.1051/parasite/2024076

Gabriel Simon, Frédéric Grenouillet, Carine Richou, Eric Delabrousse, Oleg Blagoskonov, Anne Minello, Gerard Thiefin, Emilia Frentiu, Martine Wallon, Solange Bresson-Hadni, Paul Calame

{"title":"Correlation between imaging features and rEm18 antibodies in alveolar echinococcosis: results from a multicenter study in France.","authors":"Gabriel Simon, Frédéric Grenouillet, Carine Richou, Eric Delabrousse, Oleg Blagoskonov, Anne Minello, Gerard Thiefin, Emilia Frentiu, Martine Wallon, Solange Bresson-Hadni, Paul Calame","doi":"10.1051/parasite/2024076","DOIUrl":"10.1051/parasite/2024076","url":null,"abstract":"Background: To correlate imaging features of alveolar echinococcosis (AE) with the antibodies to recombinant Em18 (rEm18AB) at diagnosis and evaluate initial imaging features associated with serologic response, this retrospective study used data from the prospective multicenter EchinoVISTA study (NCT02876146). Imaging and serology were performed at diagnosis and during follow-up. Univariate and multivariate analyses were used to evaluate imaging features associated with the rEm18AB index. Follow-up analyses evaluated the imaging features associated with serologic response (defined as a 50% reduction in the baseline value within 2 years) in non-operated patients treated with albendazole alone.Results: From June 2012 to July 2016, 45 patients were included, with 8/45 (18%) having an rEm18AB index < 1. Maximum lesion size (76 mm [IQR = 57-93] vs. 36 mm [IQR = 26-51], p = 0.006), microcyst percentage (70% [IQR = 3-8] vs. 20% [IQR = 0.5-3.5], p = 0.004), and maximum standardized uptake value (SUV) on fluorodeoxyglucose positron emission tomography (5.1 [IQR = 4.4-6.2] vs. 2.6 [IQR = 2.4-3.9], p = 0.001) were associated with an rEm18AB index > 1. In patients treated with albendazole, serologic responders at 2 years had smaller lesions (5.3 [IQR = 3.8-72] vs. 3.5 [IQR = 2.7-3.7], p = 0.010) with less pedicle involvement, and lower initial rEm18AB index (2.98 ± 1.63 vs. 7.81 ± 3.95, p = 0.011).Conclusion: Maximum lesion size, percentage of microcysts within the lesion, and maximum lesion SUV are significant imaging features of AE correlated with the rEm18AB index. Serologic response at 2 years occurs primarily in patients with small lesions and a low rEm18AB index.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"32 ","pages":"8"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11804184/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143365559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular detection of parasites and host preference in wild-caught Culicoides biting midges (Diptera: Ceratopogonidae) in Chiang Mai and Nakhon Si Thammarat Provinces, Thailand. 泰国清迈和那空府野生库蠓（双翅目：蠓科）寄生虫分子检测及寄主偏好

IF 2.3 2区医学

Parasite Pub Date : 2025-01-01 Epub Date: 2025-01-22 DOI: 10.1051/parasite/2024082

Baby Kyi Soe, Saowalak Kaewmee, Chonlada Mano, Urassaya Pattanawong, Nopporn Tipparawong, Padet Siriyasatien, Derek Gatherer, Michael D Urbaniak, Paul A Bates, Narissara Jariyapan

{"title":"Molecular detection of parasites and host preference in wild-caught Culicoides biting midges (Diptera: Ceratopogonidae) in Chiang Mai and Nakhon Si Thammarat Provinces, Thailand.","authors":"Baby Kyi Soe, Saowalak Kaewmee, Chonlada Mano, Urassaya Pattanawong, Nopporn Tipparawong, Padet Siriyasatien, Derek Gatherer, Michael D Urbaniak, Paul A Bates, Narissara Jariyapan","doi":"10.1051/parasite/2024082","DOIUrl":"10.1051/parasite/2024082","url":null,"abstract":"Culicoides biting midges (Diptera: Ceratopogonidae) have been reported as potential vectors for haemoparasites. Information about host-vector-parasite specificity is required to confirm their status. Here, molecular detection of haemosporidians, Leishmania, trypanosomatids, and filarial nematodes in biting midges was conducted to understand their potential role as vectors, and their host preference was determined. Wild-caught biting midges were collected from six different localities of Chiang Mai and Nakhon Si Thammarat provinces, Thailand. A total of 6,578 individual Culicoides (170 males, 6,408 females) comprising 15 species of six sub-genera and two groups were collected. Also, 738 parous females and 29 engorged females were examined for parasites and host blood meals, respectively. Culicoides arakawae, C. mahasarakhamense, C. peregrinus and C. innoxius in Chiang Mai province, and C. innoxius and C. peregrinus in Nakhon Si Thammarat province were the most dominant species. Leucocytozoon spp., Leucocytozoon caulleryi and Plasmodium juxtanucleare were identified in five Culicoides species including C. mahasarakhamense, C. arakawae, C. oxystoma, C. fulvus, and C. guttifer. This study is the first record of L. caulleryi in the biting midge C. arakawae in Thailand. Blood meal analysis revealed that Culicoides primarily fed on cattle (17/29, 58.6%), followed by chickens (10/29, 34.5%), and humans (2/29, 6.9%). Our findings confirmed the existence of several Culicoides species in Thailand, which might be potential vectors for transmission of haemosporidians (Leucocytozoon and Plasmodium). Information from host blood meal analyses underlined their preference for large mammals, followed by domestic chickens. More anthropophilic Culicoides species remain to be discovered.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"32 ","pages":"2"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11752737/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143009449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Challenges and lessons from a vector control campaign targeting Glossina palpalis palpalis in an isolated protected forest area in Abidjan, Côte d'Ivoire. 在阿比让（Côte科特迪瓦）一个孤立的森林保护区开展针对palpalis glssina palpalis palpalis病媒控制运动的挑战和经验教训。

IF 2.3 2区医学

Parasite Pub Date : 2025-01-01 Epub Date: 2025-04-15 DOI: 10.1051/parasite/2025017

Yao Jean Rodrigue Konan, Bi Tra Dieudonné Ta, Djakaridja Berté, Bamoro Coulibaly, Kinifo Donatien Coulibaly, Nick Steven Egnankon, Foungniguée Diarrassouba, Kouassi Albert Djabo, Stéphanie Watier-Grillot, Jean-Paul Demoncheaux, Koffi Alain De Marie Kouadio, Louis N'Dri, Philippe Solano, Sophie Ravel, Guy Pacôme Adingra, Antoine Barreaux, Adeline Ségard, Dramane Kaba, Vincent Jamonneau, Thierry De Meeûs, Vincent Djohan

{"title":"Challenges and lessons from a vector control campaign targeting Glossina palpalis palpalis in an isolated protected forest area in Abidjan, Côte d'Ivoire.","authors":"Yao Jean Rodrigue Konan, Bi Tra Dieudonné Ta, Djakaridja Berté, Bamoro Coulibaly, Kinifo Donatien Coulibaly, Nick Steven Egnankon, Foungniguée Diarrassouba, Kouassi Albert Djabo, Stéphanie Watier-Grillot, Jean-Paul Demoncheaux, Koffi Alain De Marie Kouadio, Louis N'Dri, Philippe Solano, Sophie Ravel, Guy Pacôme Adingra, Antoine Barreaux, Adeline Ségard, Dramane Kaba, Vincent Jamonneau, Thierry De Meeûs, Vincent Djohan","doi":"10.1051/parasite/2025017","DOIUrl":"https://doi.org/10.1051/parasite/2025017","url":null,"abstract":"Vector control (VC) is one of the strategies employed to manage African trypanosomoses. This study aimed at assessing the effectiveness of a VC campaign against Glossina palpalis palpalis using tiny targets (TTs) impregnated with insecticide in an isolated, protected forest in Abidjan, Côte d'Ivoire, while considering ecological, genetic, and operational factors. Between January 2020 and September 2022, 2,712 TTs were deployed at 684 sites, covering a total area of 1.7 km2. VC monitoring was conducted using Vavoua traps during 12 evaluation surveys, between June 2020 and March 2023. Five months after the initial TT deployment, tsetse fly density had decreased by 98.53%. Although tsetse density remained low due to TT redeployment and reinforcement, there was a significant increase a few months after the last redeployment. VC appeared to have minimal impact on the genetic structuring of G. p. palpalis. This suggested recruitment of local surviving tsetse flies all along the VC campaign due to a low probability of tsetse coming into contact with TTs, or to the evolution of behavioral or physiological resistance to control efforts. The genetic study revealed that one of the microsatellite markers used, the GPCAG locus, exhibited a selection signature possibly in response to VC. This could partly explain the challenges encountered in eliminating a seemingly isolated tsetse population thriving in a particularly favorable habitat.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"32 ","pages":"25"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12002674/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144019597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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