Parasite最新文献

{"title":"Wild sympatric rodents inhabiting pig farm environments may facilitate the spillover of Enterocytozoon bieneusi from pig farms.","authors":"Fa Shan, Qingda Meng, Fang Wang, Jinfeng Zhao, Huiyan Xu, Nanhao Wang, Yufeng Liu, Sumei Zhang, Guanghui Zhao, Longxian Zhang","doi":"10.1051/parasite/2024061","DOIUrl":"https://doi.org/10.1051/parasite/2024061","url":null,"abstract":"<p><p>Enterocytozoon bieneusi is a zoonotic pathogen prevalent in mammalian and avian hosts across the globe. Wild small mammals, being abundant worldwide, serve as important sources of zoonotic disease transmission to humans. Here, 227 fecal samples were collected from five rodent and shrew species on 34 pig farms in China to investigate the prevalence and molecular characterization of E. bieneusi. The overall prevalence of E. bieneusi was 17.18% (39/227), with a distribution of 23.53% (32/136) in Rattus tanezumi, 8.62% (5/58) in Rattus norvegicus, and 8.00% (2/25) in Mus musculus. Eight E. bieneusi genotypes were identified, comprising four known genotypes: D (n = 8), EbpC (n = 8), PigEBITS7 (n = 9), and EbpA (n = 2), and four novel genotypes: CHPR1 (n = 7), CHPR2 (n = 1), CHPR3 (n = 2), and CHPR4 (n = 2). This study is the first to report E. bieneusi in rodents from pig farms in Henan, Shaanxi, and Shanxi Provinces in China. The host range of genotype EbpC was expanded with its first detection in M. musculus and R. tanezumi. All identified E. bieneusi genotypes belong to group 1, raising concerns about these sympatric rodents being reservoirs of zoonotic transmission. Moreover, the widespread distribution of genotype EbpC suggests potential cross-species transmission between sympatric rodents and domestic pigs. Our findings highlight the potential role of sympatric rodents in facilitating the spillover of E. bieneusi from pig farms, which could pose a potential public health threat.</p>","PeriodicalId":19796,"journal":{"name":"Parasite","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11433834/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142351528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spatio-temporal patterns of stomoxyine flies (Diptera: Muscidae) in a forested area of Thailand.","authors":"Watcharadol Yeohsakul, Tanasak Changbunjong, Suppada Kananub, Saree Nakbun, Jumnongjit Phasuk, Supaphen Sripiboon","doi":"10.1051/parasite/2024062","DOIUrl":"10.1051/parasite/2024062","url":null,"abstract":"<p><p>Understanding the distribution patterns of vector populations is crucial for comprehending the dynamics of vector-borne diseases. However, data on vector composition and abundance in areas of forest and wildlife-human interface in Thailand remain limited. This research aimed to investigate the spatio-temporal distribution and species diversity of stomoxyine flies (Diptera: Muscidae) in Salakpra Wildlife Sanctuary, Thailand's first wildlife sanctuary. A longitudinal entomological survey was conducted monthly from May 2022 to April 2023 in four habitats: core forest, grassland forest, a wildlife breeding center, and a local cattle farm. A total of 11,256 stomoxyine flies from four genera were captured. Based on morphological keys, nine species of stomoxyine flies were identified: Stomoxys pullus (29.63%), Stomoxys calcitrans (19.65%), Stomoxys indicus (16.09%), Haematostoma austeni (14.23%), Haematobia irritans exigua (8.22%), Haematobosca sanguinolenta (7.96%), Stomoxys uruma (1.98%), Stomoxys sitiens (1.75%), and Stomoxys bengalensis (0.49%). Heterogeneous variations in abundance across months and habitats were observed, in which abundance increased in the rainy season (June-October), exhibiting bimodal peaks at seasonal transitions. Human-disturbed areas, such as the cattle farm, exhibited the highest density and species diversity of stomoxyine flies. In contrast, areas with minimal human disturbance, like core forest, had low diversity and density but supported unique species, like the abundant Haematostoma austeni, which had minor populations in other types of habitats. The results of this study can be integrated into epidemiological models and lay the groundwork for more comprehensive research on vector-borne diseases at the wildlife-livestock interface to mitigate transmission risks and preserve biodiversity.</p>","PeriodicalId":19796,"journal":{"name":"Parasite","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11451186/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142372535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular investigation of Blastocystis sp. infections in wild rodents from the Inner Mongolian Autonomous Region and Liaoning province, China: High prevalence and dominance of ST4.","authors":"Li Liu, Long Wang, Feng Tan, Wei Zhao, Fansheng Zeng","doi":"10.1051/parasite/2024031","DOIUrl":"10.1051/parasite/2024031","url":null,"abstract":"<p><p>Wild rodents are key carriers of various human pathogens, including Blastocystis spp. Our study aimed to assess the prevalence and genetic characteristics of Blastocystis among wild rodents in the Inner Mongolian Autonomous Region and Liaoning Province of China. From November 2023 to February 2024, 486 rodents were captured in these regions. Fresh feces were collected from the intestines of each rodent for the isolation of DNA and PCR amplification of the vertebrate cytochrome b (cytb) gene to identify rodent species. Subsequently, PCR analysis and sequencing of the partial small subunit of the ribosomal RNA (rRNA) gene were utilized to detect Blastocystis in all fecal samples. Of the total samples, 27.4% (133/486) were found to be Blastocystis positive. The results revealed the presence of four species of rodents infected with Blastocystis, 32.3% (63/195) in Rattus norvegicus, 15.1% (16/106) in Mus musculus, 20.2% (18/89) in Apodemus agrarius, and 37.5% (36/96) in Cricetulus barabensis. Sequence analysis confirmed the existence of five Blastocystis subtypes: ST1 (n = 4), ST2 (n = 2), the ST4 (n = 125, the dominant subtype), ST10 (n = 1), and a novel ST (n = 1). The identified zoonotic subtypes (ST1, ST2, ST4, and ST10) highlight the possible role played by wild rodents in the transmission of Blastocystis to humans, thereby elevating the chances of human infection. Meanwhile, the discovery of novel sequences also provides new insights into the genetic diversity of this parasite.</p>","PeriodicalId":19796,"journal":{"name":"Parasite","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11195528/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141443172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cystic echinococcosis (Echinococcus granulosus sensu lato infection) in Tunisia, a One Health perspective for a future control programme.","authors":"Mohamed Gharbi, Patrick Giraudoux","doi":"10.1051/parasite/2024029","DOIUrl":"10.1051/parasite/2024029","url":null,"abstract":"<p><p>The emergence of pandemics with dramatic consequences for human health has obscured endemic diseases that continue to pose a problem for human and animal health in several regions of the world. Among these diseases, cystic echinococcosis, a zoonotic parasitic infection caused by a group of cestodes, Echinococcus granulosus sensu lato, remains a real human and animal health problem in several regions of the world, including the Mediterranean Basin. Despite the implementation of a number of governmental control programmes using several tools (dog treatment, meat inspection, etc.), this infection is still highly prevalent in North Africa. Here we present a review of the epidemiology of cystic echinococcosis in Tunisia, an analysis of the constraints limiting the effectiveness of the control programmes implemented, and finally argue for the use of the One Health framework to improve the effectiveness of future programmes.</p>","PeriodicalId":19796,"journal":{"name":"Parasite","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11177845/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141317959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative performance of ISAGA IgM and ELISA assays for the diagnosis of maternal and congenital Toxoplasma infections: which technique could replace ISAGA IgM?","authors":"Anne-Sophie Deleplancque, Hélène Fricker-Hidalgo, Christelle Pomares, Coralie L'Ollivier, Jean-Philippe Lemoine, Bernard Cimon, Luc Paris, Sandrine Houzé, Isabelle Villena, Hervé Pelloux, Odile Villard","doi":"10.1051/parasite/2024004","DOIUrl":"10.1051/parasite/2024004","url":null,"abstract":"<p><p>The ISAGA immunocapture test for the detection of anti-Toxoplasma immunoglobulin M is a manual technique known for its excellent sensitivity and specificity. The purpose of this retrospective, multicenter study was to compare the performances and agreement between ISAGA and other IgM detection techniques before cessation of ISAGA production. The analytic performance of the different tests was evaluated using 1,341 serum samples from adults with positive IgM and negative IgG to Toxoplasma gondii, and 1,206 sera from neonates born to mothers with seroconversion. The agreement between the tests was evaluated on 13,506 adult and 5,795 child serum samples. The sensitivity of Toxo-ISAGA IgM^® (adults 98.7%, neonates 63.1%) was similar to that of Platelia Toxo IgM^® (adults 94.4%, neonates 64.6%), and significantly higher than Liaison Toxo IgM^® (adults 90.6%), Architect/Alinity Toxo IgM^® (adults 95.7%, neonates 48.6%), and Vidas Toxo IgM^® (adults 81.8%, neonates 17.5%). However, the specificities varied between 24.4% (Platelia Toxo IgM^®) and 95.2% (Liaison Toxo IgM^®) in adults and were >95% for all tests in neonates. An analysis of the kappa coefficients showed better agreement between ISAGA IgM^® and the other tests in children (0.75-0.83%) than in adults (0.11-0.53%). We conclude that, in the absence of Toxo-ISAGA IgM^®, the association of a very sensitive technique (Platelia Toxo IgM^® or Architect/Alinity Toxo IgM^®) and a very specific technique (Vidas Toxo IgM^® or Liaison Toxo IgM^®) is recommended for IgM detection in adult sera. For neonates, Platelia Toxo IgM^® appeared to be the best alternative to replace Toxo-ISAGA IgM^®.</p>","PeriodicalId":19796,"journal":{"name":"Parasite","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10854481/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139707507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clonorchis sinensis infection induces pathological changes in feline bile duct epithelium and alters biliary microbiota composition.","authors":"Feng Li, Yanli Zhang, Chunfu Li, Fenqi Li, Baojiang Gan, Hong Yu, Jian Li, Xinyu Feng, Wei Hu","doi":"10.1051/parasite/2024053","DOIUrl":"10.1051/parasite/2024053","url":null,"abstract":"<p><strong>Background: </strong>Clonorchis sinensis is a zoonotic liver fluke that inhabits the bile ducts of the human liver for prolonged periods, leading to cholangiocarcinoma. Recent research indicates associations between altered biliary microbiota and bile duct disorders. However, the impacts of C. sinensis infection on bile duct epithelium and subsequent effects on biliary microbiota remain unknown.</p><p><strong>Methods: </strong>Feline bile duct samples were collected from both uninfected and C. sinensis-infected cats. Histopathological examination was performed to assess epithelial changes, fibrosis, mucin and cell proliferation using hematoxylin-eosin staining and immunohistochemistry. Additionally, biliary microbiota composition was analyzed through 16S rRNA gene sequencing. Statistical analyses were conducted to compare the microbial diversity and relative abundance between infected and uninfected samples.</p><p><strong>Results: </strong>Histopathological analysis of infected feline bile ducts revealed prominent epithelial hyperplasia characterized by increased cell proliferation. Moreover, periductal fibrosis and collagen fibrosis were observed in infected samples compared to uninfected controls. Biliary microbial richness decreased with disease progression compared to uninfected controls. Streptococcus abundance positively correlated with disease severity, dominating communities in cancer samples. Predictive functional analysis suggested that C. sinensis may promote bile duct lesions by increasing microbial genes for carbohydrate metabolism, replication, and repair.</p><p><strong>Conclusions: </strong>This study provides comprehensive insights into the pathological effects of C. sinensis infection on feline bile duct epithelium and its influence on biliary microbiota composition. These novel findings provide insight into C. sinensis pathogenesis and could inform therapeutic development against human clonorchiasis. Further research is warranted to elucidate the underlying mechanisms driving these changes and their implications for host-parasite interactions.</p>","PeriodicalId":19796,"journal":{"name":"Parasite","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11378715/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142140750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hemolivia species infecting Central American wood turtles (Rhinoclemmys pulcherrima manni) and problems with differential diagnosis within the genus Hemolivia.","authors":"Žaneta Živčicová, Jana Kvičerová, Pavel Široký","doi":"10.1051/parasite/2023067","DOIUrl":"10.1051/parasite/2023067","url":null,"abstract":"<p><p>Blood parasites of the genus Hemolivia Petit, Landau, Baccam and Lainson, 1990 (Adeleorina: Karyolysidae) are hemogregarines of ectothermic vertebrates, such as lizards, chelonians, and toads. Only five species of Hemolivia from vertebrate hosts and one from their tick vector have been described so far. In the present study, Central American wood turtles (Rhinoclemmys pulcherrima manni) originating from Southern Nicaragua were screened for the presence of hemogregarines. Ten out of 30 specimens (33.3%) were positive for Hemolivia using both approaches - microscopy and PCR-based analyses. Phylogenetic analyses based on the 18S rRNA gene revealed the presence of two haplotypes, both placed as sister taxa in the Hemolivia clade. Their phylogenetic position was supported by high bootstrap values and high posterior probabilities, suggesting that there are at least two new distinct haplotypes corresponding to two distinct species. However, the specimens of each haplotype were microscopically indistinguishable from each other based on the gamont morphology, therefore, only a single species could be described and named, as Hemolivia pulcherrima n. sp. We consider that the uniform morphology of the most common blood stages of species of the genus Hemolivia complicates their differential diagnosis. Sequence divergence and different host spectra, therefore, remain the only differentiating tools.</p>","PeriodicalId":19796,"journal":{"name":"Parasite","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10865994/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139730226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Animal trypanosomosis eliminated in a major livestock production region in Senegal following the eradication of a tsetse population.","authors":"Momar Talla Seck, Assane Guèye Fall, Mamadou Ciss, Mame Thierno Bakhoum, Baba Sall, Adji Marème Gaye, Geoffrey Gimonneau, Mireille Djimangali Bassène, Renaud Lancelot, Marc J B Vreysen, Jérémy Bouyer","doi":"10.1051/parasite/2024010","DOIUrl":"10.1051/parasite/2024010","url":null,"abstract":"<p><p>African animal trypanosomosis (AAT) was one of the main disease-related constraints to the development of intensive livestock production systems in the Niayes region of Senegal, a 30 km wide strip of land along the coast between Dakar and Saint-Louis. To overcome this constraint, the Government of Senegal initiated an area-wide integrated pest management programme combining chemical control tactics with the sterile insect technique to eradicate a population of the tsetse fly Glossina palpalis gambiensis Vanderplank, 1949 (Diptera, Glossinidae) in this area. The project was implemented following a phased conditional approach, and the target area was divided into three blocks treated sequentially. This study aims to assess the temporal dynamics of the prevalence of Trypanosoma spp. during the implementation of this programme. Between 2009 and 2022, 4,359 blood samples were collected from cattle and screened for trypanosomes using both the buffy coat and ELISA techniques, and PCR tests since 2020. The seroprevalence decreased from 18.9% (95%CI: 11.2-26.5) in 2009 to 0% in 2017-2022 in block 1, and from 92.9% (95%CI: 88.2-97) in 2010 to 0% in 2021 in block 2. The parasitological and serological data confirm the entomological monitoring results, i.e., that there is a high probability that the population of G. p. gambiensis has been eradicated from the Niayes and that the transmission of AAT has been interrupted in the treated area. These results indicate the effectiveness of the adopted approach and show that AAT can be sustainably removed through the creation of a zone free of G. p. gambiensis.</p>","PeriodicalId":19796,"journal":{"name":"Parasite","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10918644/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140050029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-locus sequence analysis unveils a novel genus of filarial nematodes associated with ticks in French Guiana.","authors":"Marjorie Bruley, Olivier Duron","doi":"10.1051/parasite/2024015","DOIUrl":"10.1051/parasite/2024015","url":null,"abstract":"<p><p>Filarial nematodes of the Dipetalonema lineage include tick-borne filarioids that infect both domestic and wild vertebrate hosts, but they remain understudied in many cases. In this study, we conducted a molecular characterization of a Dipetalonema-like filarioid (DLF) recently identified in two tick species in French Guiana, South America. While the cox1 mitochondrial gene was the sole marker initially sequenced for describing DLF, its classification and phylogenetic relationship with other members of the Dipetalonema lineage were unclear. Therefore, we better characterized DLF through the sequencing of six additional gene markers and conducted phylogenetic analyses. Based on this multi-locus typing scheme, DLF exhibited significant divergence from known genera and species of filarioids, or other sequences available in public databases, suggesting its potential classification as a novel genus within the Dipetalonema lineage. Phylogenetic analyses further unveiled a close evolutionary relationship between DLF and all other filarioids associated with Acari (ticks and mites) within a robust monophyletic subclade in the Dipetalonema lineage. Overall, these findings confirm the existence of a specialized, Acari-borne group of filarioids and underscore the need for comprehensive investigations into their epidemiology and potential impact on animal health.</p>","PeriodicalId":19796,"journal":{"name":"Parasite","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10941835/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140132172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Colonization of Anopheles coustani, a neglected malaria vector in Madagascar.","authors":"Tsarasoa M Andrianinarivomanana, Fenomiaranjara T Randrianaivo, Mandaniaina R Andriamiarimanana, Mihary R Razafimamonjy, Haja J S Velonirina, Nicolas Puchot, Romain Girod, Catherine Bourgouin","doi":"10.1051/parasite/2024032","DOIUrl":"10.1051/parasite/2024032","url":null,"abstract":"<p><p>Anopheles coustani has long been recognized as a secondary malaria vector in Africa. It has recently been involved in the transmission of both Plasmodium falciparum and P. vivax in Madagascar. As most secondary malaria vectors, An. coustani mainly bites outdoors, which renders the control of this mosquito species difficult using classical malaria control measures, such as the use of bed nets or indoor residual spraying of insecticides. For a better understanding of the biology and vector competence of a vector species, it is useful to rear the species in the laboratory. The absence of a colony hinders the assessment of the bionomics of a species and the development of adapted control strategies. Here, we report the first successful establishment of an An. coustani colony from mosquitoes collected in Madagascar. We used a forced copulation procedure as this mosquito species will not mate in cages. We describe our mosquito colonization procedure with detailed biological features concerning larval to adult development and survival, recorded over the first six critical generations. The procedure should be easily applicable to An. coustani from different African countries, facilitating local investigation of An. coustani vector competence and insecticide resistance using the colony as a reference.</p>","PeriodicalId":19796,"journal":{"name":"Parasite","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11186460/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141420274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}