Parasite最新文献_第7页

No evidence of Alzheimer's disease pathology in mice infected with Toxocara canis. 在感染犬弓形虫的小鼠中没有阿尔茨海默病的病理证据。

IF 2.3 2区医学

Parasite Pub Date : 2025-01-01 Epub Date: 2025-04-09 DOI: 10.1051/parasite/2025019

Ondřej Vosála, Barbora Šmídová, Jan Novák, Jan Svoboda, Tomáš Petrásek, Iveta Vojtěchová, Tomáš Macháček

{"title":"No evidence of Alzheimer's disease pathology in mice infected with Toxocara canis.","authors":"Ondřej Vosála, Barbora Šmídová, Jan Novák, Jan Svoboda, Tomáš Petrásek, Iveta Vojtěchová, Tomáš Macháček","doi":"10.1051/parasite/2025019","DOIUrl":"https://doi.org/10.1051/parasite/2025019","url":null,"abstract":"The potential link between the infections and the development of Alzheimer's disease (AD) has led to speculations about the role of various pathogens in triggering amyloid-β (Aβ) overproduction, possibly leading to AD onset. The globally distributed dog roundworm Toxocara canis was suggested to be a suitable candidate due to neurotropism of the larvae and infection chronicity. This study investigated whether chronic T. canis infection induces AD-like pathology in mice and whether Aβ is toxic to T. canis. BALB/c and APP/PS1 transgenic mice, which overproduce Aβ, were infected with T. canis L3 larvae and monitored for larval burden, Aβ accumulation, and behavioral changes. In vitro tests of recombinant Aβ toxicity against the larvae were also performed. Despite the presence of T. canis larvae in the central nervous system 8 and 16 weeks post-infection, no significant increase in Aβ concentration or AD-related behavioral alterations were observed. Aβ was detected on the surface and within the intestines of T. canis larvae, but in vitro exposure to recombinant Aβ did not affect larval viability or morphology. Our findings suggest that T. canis infection does not trigger AD-like pathology in mice, and Aβ does not act as an antiparasitic agent. This challenges the emerging hypothesis that chronic neurotoxocarosis infections may contribute to AD development.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"32 ","pages":"24"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11987500/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144008598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Apoptotic proteins in Leishmania donovani: in silico screening, modeling, and validation by knock-out and gene expression analysis. 多诺瓦利什曼原虫的凋亡蛋白：通过敲除和基因表达分析筛选，建模和验证。

IF 2.3 2区医学

Parasite Pub Date : 2025-01-01 Epub Date: 2025-02-12 DOI: 10.1051/parasite/2024081

Ketan Kumar, Lucien Crobu, Rokhaya Thiam, Chandi C Mandal, Yvon Sterkers, Vijay Kumar Prajapati

{"title":"Apoptotic proteins in Leishmania donovani: in silico screening, modeling, and validation by knock-out and gene expression analysis.","authors":"Ketan Kumar, Lucien Crobu, Rokhaya Thiam, Chandi C Mandal, Yvon Sterkers, Vijay Kumar Prajapati","doi":"10.1051/parasite/2024081","DOIUrl":"10.1051/parasite/2024081","url":null,"abstract":"Visceral leishmaniasis, a life-threatening vector-borne illness that disproportionately affects children and elderly immunocompromised people, is a primary tropical neglected disease. No apoptotic partner proteins have yet been reported in Leishmania donovani, while their identification could contribute to knowledge on parasite cell death and the establishment of alternative therapeutics. We searched for mammalian Bcl-2 family protein orthologs and found one anti-apoptotic and two pro-apoptotic orthologs in L. donovani. A pro-death aquaporin protein, due to its characteristic BH3 domain known to interact with pro-apoptotic proteins in mammalian Bcl-2 family proteins, was also included in this study. Molecular docking and molecular dynamics simulations were conducted to assess protein-protein interactions between the identified apoptotic proteins and mimic mammalian intrinsic apoptotic pathways. The results showed that both pro-apoptotic proteins interacted with the hydrophobic pocket of the anti-apoptotic ortholog, forming a stable complex. This interaction may represent a critical event in an apoptotic pathway in L. donovani. To further characterise it, we used CRISPR-Cas9 approaches to target the identified proteins. Pure knocked population mutants, and episomal over-expressing mutant cells were exposed to apoptotic stimuli. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay and quantitative expression profiling suggested that these proteins are involved in the parasite's apoptosis and could play a role in its survival.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"32 ","pages":"9"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11825125/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143414805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Toxocara canis infection on liver and lung microbial flora diversity and composition in dogs. 犬弓形虫感染对犬肝脏和肺部微生物菌群多样性和组成的影响。

IF 2.3 2区医学

Parasite Pub Date : 2025-01-01 Epub Date: 2025-03-05 DOI: 10.1051/parasite/2025011

Na Wang, Soben Sieng, Tian Liang, Ping Chen, Jingyun Xu, Qian Han

{"title":"Effect of Toxocara canis infection on liver and lung microbial flora diversity and composition in dogs.","authors":"Na Wang, Soben Sieng, Tian Liang, Ping Chen, Jingyun Xu, Qian Han","doi":"10.1051/parasite/2025011","DOIUrl":"10.1051/parasite/2025011","url":null,"abstract":"Toxocariasis is a zoonotic parasitic disease that is widely prevalent in the world. Toxocara canis adults are parasitic in the small intestinal tract of canids, and the larvae migrate to the liver and lungs before reaching the final destination. Our previous experiments have confirmed that T. canis infection could affect the composition of host intestinal microbial flora. In this experiment, we further analyze the potential effects of T. canis infection on host liver and lung microbial flora. Utilizing 16s rRNA high-throughput sequencing, coupled with various bioinformatics analysis techniques, our study revealed that T. canis infection significantly elevated the abundance of certain opportunistic pathogens in the host's liver and lungs. This marked elevation contributes to the establishment of infection. Through cluster analysis, we found that the changes in the microbiota of the liver and lungs were independent of the microbial flora carried by T. canis adults. However, whether the changes are due to the migration of larvae remains to be explored. In short, T. canis infections have a significant impact on the abundance and diversity of flora in the host tissues, and the changes in microbiota abundance and diversity could further influence tissue homeostasis and immune responses, thus regulating the establishment of infection.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"32 ","pages":"17"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11882136/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143567842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A review of Leishmania infections in American Phlebotomine sand flies - Are those that transmit leishmaniasis anthropophilic or anthropportunists?★. 美国白蛉感染利什曼病的综述——传播利什曼病的人是亲人类还是机会主义者？

IF 2.4 2区医学

Parasite Pub Date : 2025-01-01 Epub Date: 2025-09-08 DOI: 10.1051/parasite/2025039

Jeffrey Jon Shaw

{"title":"A review of Leishmania infections in American Phlebotomine sand flies - Are those that transmit leishmaniasis anthropophilic or anthropportunists?★.","authors":"Jeffrey Jon Shaw","doi":"10.1051/parasite/2025039","DOIUrl":"10.1051/parasite/2025039","url":null,"abstract":"Understanding why Diptera, such as mosquitoes and sand flies, feed on humans is crucial in defining them as vectors of diseases such as malaria, dengue fever, Zika virus, and leishmaniasis. Determining their attraction to humans (anthropophily) helps in assessing the risk of disease transmission, designing effective vector control strategies, and monitoring the effectiveness of existing control measures. An important question is whether they are specifically attracted to humans in preference to other mammals or whether there is something else at play. In this paper, I propose that the idea that saying species are \"anthropophilic\" when they are preferentially attracted to humans is misleading and that such species are generalists that will opportunistically feed on a wide range of animals including humans when they are available. Other species are specialists that, under rare circumstances, accidently feed on humans. For these groups, I propose the names anthropportunists and anthroaccidentalists, respectively. The level of contact depends on a range of environmental factors and it is these that must be considered in evaluating potential vector importance and management. In this paper, I propose a review of the Leishmania identified in American sand flies and relate them to these two concepts and how it is linked to taxonomic groups, evolution, and ecology. There are records of Leishmania in 91 species, which is only 16.5% of American sand fly species. Of these infections, 56.7% are in the genera Lutzomyia, Nyssomyia, Pintomyia, and Psychodopygus, which are typically generalist anthropportunists. Of the species considered to be proven vectors, 77.4% belong to these four genera. As infections were detected by a variety of methods, further case-by-case field studies are required to evaluate the vectorial role of many of the sand fly species in which Leishmania have been found.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"32 ","pages":"57"},"PeriodicalIF":2.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12416862/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145023970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prevalence, morphological and molecular characterization of Leucocytozoon macleani (Apicomplexa: Haemosporida) from chickens in Thailand. 泰国鸡maclecytozoon（顶复合体：血孢子虫）的流行、形态和分子特征。

IF 2.4 2区医学

Parasite Pub Date : 2025-01-01 Epub Date: 2025-08-05 DOI: 10.1051/parasite/2025043

Nikom Srikacha, Carolina Romeiro Fernandes Chagas, Surya Paudel, Pornchai Pornpanom

{"title":"Prevalence, morphological and molecular characterization of Leucocytozoon macleani (Apicomplexa: Haemosporida) from chickens in Thailand.","authors":"Nikom Srikacha, Carolina Romeiro Fernandes Chagas, Surya Paudel, Pornchai Pornpanom","doi":"10.1051/parasite/2025043","DOIUrl":"10.1051/parasite/2025043","url":null,"abstract":"Leucocytozoon species are common in countries with warm climates but are an often neglected blood parasite in poultry. Although Leucocytozoon macleani is less virulent than Leucocytozoon caulleryi, it can still negatively impact production performance. In Thailand, the available reports indicate a high prevalence of Leucocytozoon spp., but detailed morphological characteristics of the parasites remain insufficiently known. In this study, Giemsa-stained blood smears and extracted genomic (g) DNA were obtained from 60 domestic chickens (Gallus gallus domesticus). Blood smears were examined for the presence of Leucocytozoon species and their morphological characteristics were examined. A total of 60 gDNA samples were used for nested-PCR amplification of the cytochrome b gene of Leucocytozoon species, followed by sequencing and phylogenetic analysis. The microscopic and molecular examinations revealed prevalence of leucocytozoonosis in chickens of 85% and 90%, respectively. Sequence analysis indicated that several infected chickens harboured multiple Leucocytozoon lineages. Leucocytozoon macleani was morphologically identified in nine samples and could be linked to the lineages GALLUS17, GALLUS34, and the new lineages GALLUS63. The found gametocytes of L. macleani morphologically resembled those reported previously, but exhibited some distinct characteristics. Phylogenetically, the lineages of L. macleani isolated in this study grouped separately from some other L. macleani lineages deposited in GenBank. In conclusion, the prevalence of Leucocytozoon infection in chickens from Northeastern Thailand was high, with frequent co-infections by multiple lineages. Leucocytozoon macleani may exhibit cryptic specification. This study is the first report of L. macleani lineages described using MalAvi database nomenclature, alongside their morphological characteristics.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"32 ","pages":"50"},"PeriodicalIF":2.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12324565/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144789641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Systematic review and meta-analysis of the global prevalence and infection risk factors of Trichomonas vaginalis. 阴道毛滴虫全球流行率及感染危险因素的系统回顾与荟萃分析。

IF 2.4 2区医学

Parasite Pub Date : 2025-01-01 Epub Date: 2025-08-27 DOI: 10.1051/parasite/2025051

Wenjie Tian, Yuhua Li, Yani Zhang, Yiming Zhang, Yiran Qin, Yalin Han, Dongxian Li, Shuai Wang, Zhenke Yang, Xiaowei Tian, Xuefang Mei, Zhenchao Zhang

{"title":"Systematic review and meta-analysis of the global prevalence and infection risk factors of Trichomonas vaginalis.","authors":"Wenjie Tian, Yuhua Li, Yani Zhang, Yiming Zhang, Yiran Qin, Yalin Han, Dongxian Li, Shuai Wang, Zhenke Yang, Xiaowei Tian, Xuefang Mei, Zhenchao Zhang","doi":"10.1051/parasite/2025051","DOIUrl":"https://doi.org/10.1051/parasite/2025051","url":null,"abstract":"Trichomoniasis is a globally prevalent sexually transmitted disease; however, comprehensive data on its global prevalence and incidence are relatively limited. In this study, we systematically retrieved relevant articles from PubMed, Google Scholar, Scopus, Ovid-Medline, and Web of Science to analyze the prevalence of Trichomonas vaginalis and its association with various infection risk factors. Among 5,437 publications released between 1992 and 2023, 425 articles focusing on the epidemiology of T. vaginalis were identified. The results revealed a global prevalence rate of T. vaginalis of 8% (95% CI: 7%-10%), with country-specific rates ranging from 1% to 35%. The prevalence of T. vaginalis was significantly higher in the behavioral subgroups, including smoking, drug use, and not using condoms, compared to the non-infection group, with a pooled odds ratio (OR) of 1.67 (95% CI: 1.39-2.0). Furthermore, the prevalence of T. vaginalis was significantly higher in the group with other sexually transmitted infections (STIs), including HIV, HSV, and Chlamydia infection compared to the non-infection group, with a pooled OR of 2.01 (95% CI: 1.48-2.72). Finally, socioeconomic factors such as being unmarried, having a low income, and unstable employment were associated with an increased risk of T. vaginalis infection, with a pooled OR of 1.36 (95% CI: 1.10-1.66). This study has significant public health relevance for the prevention and control of trichomoniasis.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"32 ","pages":"56"},"PeriodicalIF":2.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12386857/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144964202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Suppression of Aedes albopictus in Sri Lanka using the Sterile Insect Technique (SIT) with a sustained effect. 在斯里兰卡使用昆虫不育技术（SIT）抑制白纹伊蚊并取得持续效果。

IF 2.4 2区医学

Parasite Pub Date : 2025-01-01 Epub Date: 2025-09-17 DOI: 10.1051/parasite/2025050

Menaka Hapugoda, Nilmini Silva Gunawardene, Tharaka Ranathunge, Sudath Samaraweera, K Karunathilake, Bazoumana B D Sow, Gayan Parakrama Withanage, Indika Weerasinghe, Hamidou Maiga, Jeremy Bouyer

{"title":"Suppression of Aedes albopictus in Sri Lanka using the Sterile Insect Technique (SIT) with a sustained effect.","authors":"Menaka Hapugoda, Nilmini Silva Gunawardene, Tharaka Ranathunge, Sudath Samaraweera, K Karunathilake, Bazoumana B D Sow, Gayan Parakrama Withanage, Indika Weerasinghe, Hamidou Maiga, Jeremy Bouyer","doi":"10.1051/parasite/2025050","DOIUrl":"10.1051/parasite/2025050","url":null,"abstract":"Dengue fever remains a significant public health concern in Sri Lanka, leading to recurrent epidemics and imposing substantial socio-economic burdens. This study aimed to assess the efficacy of the Sterile Insect Technique (SIT) against Aedes albopictus (Skuse), the predominant dengue vector in the country, through a pilot field trial of an Integrated Vector Management (IVM) strategy including the SIT. The pilot trial was conducted in the Gampaha district, which reports the second-highest number of dengue cases in the country. A total of 3,300,000 sterile males, exposed to a 50 Gy radiation dose, were released over 33 weeks (100,000/week) within a 30-hectare release area. Entomological assessments were conducted at 115 trapping stations over a period of 71 weeks (October 2020-August 2022). Induced sterility of 98.16% in mosquito eggs was reached within the release area as compared to the control area (binomial generalized linear mixed model, deviance 2.408, df = 2, p = 0.016), indicating a notable impact of the SIT. The trial achieved nearly 98% suppression of adult vector mosquitoes, with a sustained suppression effect for 13 weeks post cessation of releases. These findings suggest that SIT can be effectively integrated as a potential additional tool into the future IVM strategy in Sri Lanka.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"32 ","pages":"59"},"PeriodicalIF":2.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12443424/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145081164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular characterization of common zoonotic protozoan parasites and bacteria causing diarrhea in dairy calves in Ningxia Hui Autonomous Region, China. 导致中国宁夏回族自治区奶牛犊牛腹泻的常见人畜共患病原生动物寄生虫和细菌的分子特征。

IF 2.3 2区医学

Parasite Pub Date : 2024-01-01 Epub Date: 2024-10-01 DOI: 10.1051/parasite/2024059

Jia-Qi Zhao, Ying-Ying Fan, Yun-Duan Lei, Ding Liu, Jun-Wei Wang, Xin Yang, Jun-Ke Song, Guang-Hui Zhao

{"title":"Molecular characterization of common zoonotic protozoan parasites and bacteria causing diarrhea in dairy calves in Ningxia Hui Autonomous Region, China.","authors":"Jia-Qi Zhao, Ying-Ying Fan, Yun-Duan Lei, Ding Liu, Jun-Wei Wang, Xin Yang, Jun-Ke Song, Guang-Hui Zhao","doi":"10.1051/parasite/2024059","DOIUrl":"10.1051/parasite/2024059","url":null,"abstract":"Diarrhea caused by zoonotic pathogens is one of the most common diseases in dairy calves, threatening the health of young animals. Humans are also at risk, in particular children. To explore the pathogens causing diarrhea in dairy calves, the present study applied PCR-based sequencing tools to investigate the occurrence and molecular characteristics of three parasites (Cryptosporidium spp., Giardia duodenalis, and Enterocytozoon bieneusi) and three bacterial pathogens (Escherichia coli, Clostridium perfringens, and Salmonella spp.) in 343 fecal samples of diarrheic dairy calves from five farms in Lingwu County, Ningxia Hui Autonomous Region, China. The total positive rate of these pathogens in diarrheic dairy calves was 91.0% (312/343; 95% CI, 87.9-94.0), with C. perfringens (61.5%, 211/343; 95% CI, 56.3-66.7) being the dominant one. Co-infection with two to five pathogens was found in 67.3% (231/343; 95% CI, 62.4-72.3) of investigated samples. There were significant differences (p < 0.05) in the positive rates of Cryptosporidium spp. and diarrheagenic E. coli among farms, age groups, and seasons. Two Cryptosporidium species (C. parvum and C. bovis) and five gp60 subtypes of C. parvum (IIdA15G1, IIdA20G1, IIdA19G1, IIdA14G1, and a novel IIdA13G1) were identified. Two assemblages (assemblage E and zoonotic assemblage A) of G. duodenalis and six ITS genotypes of E. bieneusi (J, Henan-IV, EbpC, I, EbpA, and ESH-01) were observed. Four virulence genes (eaeA, stx1, stx2, and st) of diarrheagenic E. coli and one toxin type (type A) of C. perfringens were detected. Our study enriches our knowledge on the characteristics and zoonotic potential of diarrhea-related pathogens in dairy calves.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"31 ","pages":"60"},"PeriodicalIF":2.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11444552/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142366105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel gene arrangement in the mitochondrial genome of Aspersentis megarhynchus (Acanthocephala, Echinorhynchida, Heteracanthocephalidae), and its phylogenetic implications. Aspersentis megarhynchus（棘鱼科、棘鱼属、异棘鱼科）线粒体基因组中的新基因排列及其系统发育意义。

IF 2.3 2区医学

Parasite Pub Date : 2024-01-01 Epub Date: 2024-10-08 DOI: 10.1051/parasite/2024064

Yuan-Yuan Xie, Hui-Xia Chen, Tetiana A Kuzmina, Olga Lisitsyna, Liang Li

{"title":"Novel gene arrangement in the mitochondrial genome of Aspersentis megarhynchus (Acanthocephala, Echinorhynchida, Heteracanthocephalidae), and its phylogenetic implications.","authors":"Yuan-Yuan Xie, Hui-Xia Chen, Tetiana A Kuzmina, Olga Lisitsyna, Liang Li","doi":"10.1051/parasite/2024064","DOIUrl":"10.1051/parasite/2024064","url":null,"abstract":"The Heteracanthocephalidae Petrochenko, 1956 is a rare family of acanthocephalans mainly parasitic in fishes. The pattern of mitogenomic evolution of the Heteracanthocephalidae is still unknown, and the phylogenetic relationships of the Heteracanthocephalidae with the other 14 families within the order Echinorhynchida remain unclear. In the present study, the complete mitochondrial genome of Aspersentis megarhynchus (von Linstow, 1892) Golvan, 1960 was sequenced and annotated for the first time, which represents the first mitogenomic data for the genus Aspersentis and also for the family Heteracanthocephalidae. The mitogenome of A. megarhynchus has 14,661 bp and includes 36 genes, containing 12 protein-coding genes (PCGs) (missing atp8), 22 tRNA genes, and 2 ribosomal RNAs (rrnS and rrnL), plus two non-coding regions. Comparative mitochondrial genomic analysis revealed that the presence of translocations of several tRNA genes (trnV, trnE, and trnT) and the gene arrangement in the mitogenome of A. megarhynchus represents a new type in Acanthocephala. Moreover, the mitogenomic phylogenetic results based on concatenated amino acid sequences of 12 protein-coding genes strongly supported the validity of the Heteracanthocephalidae and suggested close affinity between the Heteracanthocephalidae and Echinorhynchidae in the order Echinorhynchida.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"31 ","pages":"63"},"PeriodicalIF":2.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11460826/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142392265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of the epidemiological situation of intestinal schistosomiasis using the POC-CCA parasite antigen test and the Kato-Katz egg count test in school-age children in endemic villages in western Côte d'Ivoire. 使用 POC-CCA 寄生虫抗原检测法和 Kato-Katz 卵数检测法对科特迪瓦西部流行村落学龄儿童的肠血吸虫病流行情况进行评估。

IF 2.3 2区医学

Parasite Pub Date : 2024-01-01 Epub Date: 2024-10-29 DOI: 10.1051/parasite/2024049

Edwige A Sokouri, Bernardin Ahouty, Innocent A Abé, Flora G D Yao, Thomas K Konan, Oscar A Nyangiri, Annette MacLeod, Enock Matovu, Harry Noyes, Mathurin Koffi

{"title":"Evaluation of the epidemiological situation of intestinal schistosomiasis using the POC-CCA parasite antigen test and the Kato-Katz egg count test in school-age children in endemic villages in western Côte d'Ivoire.","authors":"Edwige A Sokouri, Bernardin Ahouty, Innocent A Abé, Flora G D Yao, Thomas K Konan, Oscar A Nyangiri, Annette MacLeod, Enock Matovu, Harry Noyes, Mathurin Koffi","doi":"10.1051/parasite/2024049","DOIUrl":"10.1051/parasite/2024049","url":null,"abstract":"Schistosomiasis is an endemic disease in Côte d'Ivoire. We compared the conventional Kato Katz (KK) test and a more sensitive but rarely used method, the point-of-care circulating cathodic antigen (POC-CCA), in order to contribute to the development of a more appropriate strategy for the control and elimination of intestinal schistosomiasis in western Côte d'Ivoire. A cross-sectional epidemiological survey was conducted in eight elementary schools in the Guémon and Cavally regions from February to December 2020. Selected schoolchildren provided stool and urine samples to detect the presence of Schistosoma mansoni eggs and parasite antigen using the KK and POC-CCA tests, respectively. A total of 554 schoolchildren were included in the study. The overall prevalence of intestinal schistosomiasis was 10% and 67% for KK and POC-CCA, respectively. The POC-CCA detected an infection rate of 100%, while the KK yielded a rate of 42%. In schools, prevalence ranged from 27 to 100% with POC-CCA and from 0 to 42% with KK. Swimming, fishing, washing clothes, and dishwashing were significantly associated with the onset of infection and high intensities. The epidemiological risk factors for intestinal schistosomiasis updated here using KK and POC-CCA diagnostic methods showed that prevalence was much higher than previously estimated using the KK. The POC-CCA is more sensitive and ways should be considered to improve its specificity in order to improve the diagnosis.","PeriodicalId":19796,"journal":{"name":"Parasite","volume":"31 ","pages":"66"},"PeriodicalIF":2.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11520599/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142522640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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