半乳甘露聚糖可抑制旋毛虫侵入肠上皮细胞,并通过驱动巨噬细胞极化增强抗体依赖性细胞毒性,从而杀死幼虫。

IF 2.3 2区 医学 Q2 PARASITOLOGY
Parasite Pub Date : 2024-01-01 Epub Date: 2024-02-08 DOI:10.1051/parasite/2024002
Ru Zhang, Yao Zhang, Shu Wei Yan, Yong Kang Cheng, Wen Wen Zheng, Shao Rong Long, Zhong Quan Wang, Jing Cui
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引用次数: 0

摘要

先前的研究表明,重组螺旋毛虫半凝集素(rTsgal)的特点是碳水化合物识别域序列基序与 beta-半乳糖苷结合,并且 rTsgal 能促进幼虫侵入肠上皮细胞。半乳甘露聚糖是一种免疫刺激多糖,由带有半乳糖残基的甘露聚糖骨架组成。本研究旨在探讨半乳甘露聚糖是否能抑制幼虫侵入肠上皮细胞并增强抗体依赖性细胞毒性(ADCC),通过将巨噬细胞极化为 M1 表型来杀死新生幼虫。结果表明,半乳甘露聚糖能与 rTsgal 特异性结合,并能减弱 rTsgal 对幼虫侵入肠上皮细胞的促进作用。qPCR、Western印迹和流式细胞术的结果表明,半乳甘露聚糖和rTsgal激活了巨噬细胞的M1极化,表现为iNOS(M1标志物)和M1相关基因(IL-1β、IL-6和TNF-α)的高表达,以及CD86+巨噬细胞的增加。半乳甘露聚糖和 rTsgal 还能增加 NO 的产生。半乳甘露聚糖和 rTsgal 处理的巨噬细胞介导的 ADCC 对幼虫的杀伤能力也显著增强。研究结果表明,Tsgal可被视为一种潜在的抗螺旋体侵袭的疫苗靶分子,半乳甘露聚糖可作为一种新型佐剂治疗剂和抗螺旋体感染的潜在疫苗佐剂。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Galactomannan inhibits Trichinella spiralis invasion of intestinal epithelium cells and enhances antibody-dependent cellular cytotoxicity related killing of larvae by driving macrophage polarization.

Previous studies have shown that recombinant Trichinella spiralis galectin (rTsgal) is characterized by a carbohydrate recognition domain sequence motif binding to beta-galactoside, and that rTsgal promotes larval invasion of intestinal epithelial cells. Galactomannan is an immunostimulatory polysaccharide composed of a mannan backbone with galactose residues. The aim of this study was to investigate whether galactomannan inhibits larval intrusion of intestinal epithelial cells and enhances antibody-dependent cellular cytotoxicity (ADCC), killing newborn larvae by polarizing macrophages to the M1 phenotype. The results showed that galactomannan specially binds to rTsgal, and abrogated rTsgal facilitation of larval invasion of intestinal epithelial cells. The results of qPCR, Western blotting, and flow cytometry showed that galactomannan and rTsgal activated macrophage M1 polarization, as demonstrated by high expression of iNOS (M1 marker) and M1 related genes (IL-1β, IL-6, and TNF-α), and increased CD86+ macrophages. Galactomannan and rTsgal also increased NO production. The killing ability of macrophage-mediated ADCC on larvae was also significantly enhanced in galactomannan- and rTsgal-treated macrophages. The results demonstrated that Tsgal may be considered a potential vaccine target molecule against T. spiralis invasion, and galactomannan may be a novel adjuvant therapeutic agent and potential vaccine adjuvant against T. spiralis infection.

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来源期刊
Parasite
Parasite 医学-寄生虫学
CiteScore
5.50
自引率
6.90%
发文量
49
审稿时长
3 months
期刊介绍: Parasite is an international open-access, peer-reviewed, online journal publishing high quality papers on all aspects of human and animal parasitology. Reviews, articles and short notes may be submitted. Fields include, but are not limited to: general, medical and veterinary parasitology; morphology, including ultrastructure; parasite systematics, including entomology, acarology, helminthology and protistology, and molecular analyses; molecular biology and biochemistry; immunology of parasitic diseases; host-parasite relationships; ecology and life history of parasites; epidemiology; therapeutics; new diagnostic tools. All papers in Parasite are published in English. Manuscripts should have a broad interest and must not have been published or submitted elsewhere. No limit is imposed on the length of manuscripts, but they should be concisely written. Papers of limited interest such as case reports, epidemiological studies in punctual areas, isolated new geographical records, and systematic descriptions of single species will generally not be accepted, but might be considered if the authors succeed in demonstrating their interest.
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