Prevalence and characterization of Cryptosporidium in sika deer from Northern China.

IF 2.3 2区医学 Q2 PARASITOLOGY

Parasite Pub Date : 2025-01-01 Epub Date: 2025-06-11 DOI:10.1051/parasite/2025029

Yan Tang, Nian-Yu Xue, Yang Gao, Zhen-Qiu Gao, Hong-Di Zhuang, Guang-Rong Bao, Hong-Wei Cao, Jing Liu, Jian-Ming Li, Shuo Liu

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Abstract

Cryptosporidium spp. are important zoonotic parasites that can cause moderate to severe diarrhea in humans and animals. However, the epidemiological data of Cryptosporidium in sika deer in China need to be updated. In this study, a total of 466 fecal samples were collected from sika deer in Shandong, Jilin, Liaoning, and Heilongjiang provinces. Nested PCR was used to amplify the SSU rRNA gene to detect Cryptosporidium spp. The results showed that the overall infection rate of Cryptosporidium spp. was 14.81%, with no significant differences among regions (p = 0.05). The highest infection rate was found in Heilongjiang Province (23.60%) and the lowest in Jilin Province (10.71%). The infection rate in summer (23.61%) seemed higher than that in autumn (13.20%), but the difference was not statistically significant (p = 0.30). Notably, young sika deer showed a significantly higher infection rate (28.21%) compared to adults (10.32%) (p < 0.0001). Sequence analysis identified two Cryptosporidium species/genotypes: Cryptosporidium deer genotype (98.55%) and Cryptosporidium ubiquitum (1.45%). Subtyping revealed that the C. ubiquitum isolate belonged to the zoonotic XIIa subtype. These findings provide new insights into the prevalence and genetic diversity of Cryptosporidium in sika deer and suggest that sika deer may act as a potential reservoir for zoonotic Cryptosporidium transmission.

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中国北方梅花鹿隐孢子虫的流行及特征。

隐孢子虫是一种重要的人畜共患寄生虫，可引起人类和动物中度至重度腹泻。然而，中国梅花鹿隐孢子虫的流行病学资料有待更新。本研究在山东、吉林、辽宁和黑龙江四省采集梅花鹿粪便样本466份。采用巢式PCR扩增SSU rRNA基因检测隐孢子虫，结果显示隐孢子虫总感染率为14.81%，区域间差异无统计学意义（p = 0.05）。感染率最高的是黑龙江省（23.60%），最低的是吉林省（10.71%）。夏季感染率（23.61%）明显高于秋季感染率（13.20%），但差异无统计学意义（p = 0.30）。值得注意的是，幼梅花鹿的感染率（28.21%）明显高于成年梅花鹿（10.32%）

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来源期刊

Parasite 医学-寄生虫学

CiteScore

5.50

自引率

6.90%

发文量

审稿时长

3 months

期刊介绍： Parasite is an international open-access, peer-reviewed, online journal publishing high quality papers on all aspects of human and animal parasitology. Reviews, articles and short notes may be submitted. Fields include, but are not limited to: general, medical and veterinary parasitology; morphology, including ultrastructure; parasite systematics, including entomology, acarology, helminthology and protistology, and molecular analyses; molecular biology and biochemistry; immunology of parasitic diseases; host-parasite relationships; ecology and life history of parasites; epidemiology; therapeutics; new diagnostic tools. All papers in Parasite are published in English. Manuscripts should have a broad interest and must not have been published or submitted elsewhere. No limit is imposed on the length of manuscripts, but they should be concisely written. Papers of limited interest such as case reports, epidemiological studies in punctual areas, isolated new geographical records, and systematic descriptions of single species will generally not be accepted, but might be considered if the authors succeed in demonstrating their interest.