Prevalence and genetic diversity of Pentatrichomonas hominis in pig populations in Guangdong and Anhui Provinces, China.

IF 2.3 2区医学 Q2 PARASITOLOGY

Parasite Pub Date : 2025-01-01 Epub Date: 2025-06-11 DOI:10.1051/parasite/2025027

Pengyun Lu, Yibin Zhu, Haiming Cai, Hanqin Shen, Siyun Fang, Dingai Wang, Zhuanqiang Yan, Shenquan Liao, Nanshan Qi, Minna Lv, Xuhui Lin, Yongle Song, Xiangjie Chen, Jianfei Zhang, Juan Li, Mingfei Sun

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Abstract

Pentatrichomonas hominis is a protozoan parasite that infects the gastrointestinal tract of humans and mammals, causing abdominal pain and diarrhea. However, its presence in pigs and its potential as a pathogen causing diarrhea in piglets have not been well studied. This study aimed to investigate the prevalence of P. hominis in pigs and its potential for zoonotic transmission. A total of 406 pig fecal samples were collected from four pig farms located in Guangdong and Anhui Provinces. Fecal DNA extraction was carried out using a commercially available kit. A nested PCR methodology was employed to detect the presence of P. hominis infection. Samples that tested positive were subsequently subjected to sequencing, and the genetic characteristics of the 18S ribosomal RNA (rRNA) gene were analyzed. The overall infection rate of P. hominis was 34.98% (142/406), in Guangdong Province 28.47% (80/281), and in Anhui Province 49.60% (62/125). Among different age groups, suckling piglets had the highest infection rate at 40.24% (68/169). Genetic analysis of the P. hominis isolates showed that the PH-1 genotype was predominant and had a high degree of similarity to P. hominis sequences obtained from humans, cats, and pigs, indicating the potential for zoonotic transmission. The high infection rate and genetic diversity highlight the need for effective control measures in pig farming to reduce parasite transmission and zoonotic risk.

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广东和安徽猪群人五毛单胞菌流行率及遗传多样性

人五毛单胞菌是一种感染人类和哺乳动物胃肠道的原生动物寄生虫，引起腹痛和腹泻。然而，它在猪中的存在及其作为引起仔猪腹泻的病原体的潜力尚未得到很好的研究。本研究旨在调查人形疟原虫在猪中的流行情况及其人畜共患传播的可能性。在广东和安徽的4个养猪场共采集了406份猪粪便样本。使用市售试剂盒进行粪便DNA提取。采用巢式PCR方法检测人疟原虫感染的存在。随后对检测呈阳性的样品进行测序，分析18S核糖体RNA （rRNA）基因的遗传特征。总感染率为34.98%(142/406)，广东省28.47%(80/281)，安徽省49.60%（62/125）。不同年龄组中，哺乳仔猪感染率最高，为40.24%（68/169）。对人源疟原虫分离物的遗传分析显示，PH-1基因型占主导地位，与从人、猫和猪中获得的人源疟原虫序列高度相似，表明人畜共患传播的可能性。高感染率和遗传多样性突出了养猪业需要采取有效的控制措施，以减少寄生虫传播和人畜共患风险。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Parasite 医学-寄生虫学

CiteScore

5.50

自引率

6.90%

发文量

审稿时长

3 months

期刊介绍： Parasite is an international open-access, peer-reviewed, online journal publishing high quality papers on all aspects of human and animal parasitology. Reviews, articles and short notes may be submitted. Fields include, but are not limited to: general, medical and veterinary parasitology; morphology, including ultrastructure; parasite systematics, including entomology, acarology, helminthology and protistology, and molecular analyses; molecular biology and biochemistry; immunology of parasitic diseases; host-parasite relationships; ecology and life history of parasites; epidemiology; therapeutics; new diagnostic tools. All papers in Parasite are published in English. Manuscripts should have a broad interest and must not have been published or submitted elsewhere. No limit is imposed on the length of manuscripts, but they should be concisely written. Papers of limited interest such as case reports, epidemiological studies in punctual areas, isolated new geographical records, and systematic descriptions of single species will generally not be accepted, but might be considered if the authors succeed in demonstrating their interest.