Mycorrhiza最新文献

{"title":"Transcriptomic and metabolomic analyses reveal the role of flavonoids in ectomycorrhizal symbiosis.","authors":"Jinhua Zhang, Junfeng Liang, Yanliu Chen, Mengya An, Xing Li","doi":"10.1007/s00572-026-01248-5","DOIUrl":"10.1007/s00572-026-01248-5","url":null,"abstract":"","PeriodicalId":18965,"journal":{"name":"Mycorrhiza","volume":"36 1","pages":"3"},"PeriodicalIF":3.8,"publicationDate":"2026-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146041467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Arbuscular mycorrhizal fungi mitigate cadmium toxicity in plants: A global meta-analysis.","authors":"Zahraddeen Kabir Sani, Tariq Shah, Sulaimon Basiru, Maeline Athenaise Salmon, Nabil Radouane, Jean Legeay, Mohamed Hijri","doi":"10.1007/s00572-025-01246-z","DOIUrl":"10.1007/s00572-025-01246-z","url":null,"abstract":"<p><p>Arbuscular mycorrhizal fungi (AMF) are known to alleviate cadmium (Cd) toxicity in plants; however, the conditions that maximize their efficiency remain poorly understood. While previous meta-analyses have documented general benefits of AMF in Cd-contaminated soils, none has systematically examined the interactive roles of soil pH, inoculant type, and plant biomass on Cd dynamics within the soil-plant system. Here, we present a comprehensive global meta-analysis (97 studies; >500 observations) using advanced statistical approaches, random-effects modeling, meta-regression, and structural equation modelling, to identify these key boundary conditions. AMF inoculation significantly (p < 0.0001) enhanced plant biomass, root and shoot length, and chlorophyll content, while markedly reducing shoot Cd concentration. Effects on antioxidant enzymes were variable and generally non-significant. Notably, AMF efficiency was strongly context-dependent: benefits were greater in acidic soils, and microbial consortia outperformed single-species inoculants in high-biomass plants by promoting root Cd immobilization. In contrast, total soil Cd concentration was a weak predictor of AMF effectiveness (meta-regression R² ≤ 2.03%), indicating that Cd bioavailability, largely determined by pH, is more critical than total metal load. Overall, our findings provide robust evidence that AMF symbiosis is a key bio-based strategy for mitigating Cd stress in plants. This study highlights soil pH, inoculant composition, and plant biomass as critical determinants of AMF efficiency and offers practical guidance for optimizing AMF-based phytostabilization and remediation in Cd-contaminated agroecosystems.</p>","PeriodicalId":18965,"journal":{"name":"Mycorrhiza","volume":"36 1","pages":"2"},"PeriodicalIF":3.8,"publicationDate":"2025-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145846255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Drink quickly. Mycorrhizal roots deplete water faster from wet soil.","authors":"David Püschel, Jana Rydlová, Radka Sudová, Jan Jansa, Michael Bitterlich","doi":"10.1007/s00572-025-01247-y","DOIUrl":"10.1007/s00572-025-01247-y","url":null,"abstract":"","PeriodicalId":18965,"journal":{"name":"Mycorrhiza","volume":"36 1","pages":"1"},"PeriodicalIF":3.8,"publicationDate":"2025-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12727761/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145809812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Laccaria bicolor adapts to phosphate deficiency at the developmental, transcriptional and metabolic levels.","authors":"Anita Loha, Sami Bouziri, Maria V Aparicio Chacon, Giovanna Ambrosini, Katharina Gutbrod, Peter Dörmann, Minna Kemppainen, Francis Martin, Yves Poirier","doi":"10.1007/s00572-025-01236-1","DOIUrl":"10.1007/s00572-025-01236-1","url":null,"abstract":"<p><p>The amount of soluble inorganic phosphate (Pi) in soils is typically low and limiting plant growth. Roots of trees in several forest ecosystems form association with ectomycorrhizal (ECM) fungi, where fungi forage and supply inorganic nutrients, such as Pi, in exchange for fixed carbon. While adaptations of model fungi, such as Saccharomyces cerevisiae, to Pi deficiency has been extensively studied, much less is known about how mycorrhizal fungi adapt to Pi deficiency. This study aimed to decipher how the free-living ECM Laccaria bicolor mycelium adapts to Pi deficiency. L. bicolor grown for 7 days in medium without Pi showed very low Pi and polyphosphate reserves and displayed less compact colonies with spreading hyphae. Pi deficiency resulted in approximately 1500-2000 genes being up- and down-regulated more than 2-fold compared to mycelium grown with abundant Pi, with most genes partially reverting their expression pattern in cultures spiked with Pi for 24 hours. Numerous genes involved in Pi mobilization from organic sources, such as phosphatases and ribonucleases, were induced by Pi deficiency, as well as genes involved in Pi transport, and such expression patterns correlated with increased enzymatic activities. Pi deficiency also induced the synthesis of the betaine lipid diacylglyceryl-N,N,N-trimethylhomoserine (DGTS). Several genes induced by mycorrhization, such as those encoding protease inhibitors belonging to the mycocypin family and Mycorrhizae-Induced Small Secreted Peptides (MiSSP), were also induced by Pi deficiency. Altogether, this study shows that L. bicolor can robustly respond to Pi deficiency and identifies parallels between these adaptations and those involved in mycorrhization.</p>","PeriodicalId":18965,"journal":{"name":"Mycorrhiza","volume":"35 6","pages":"71"},"PeriodicalIF":3.8,"publicationDate":"2025-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12698804/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145724866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proteomic insight into the ectomycorrhizal fungus Laccaria bicolor illuminates the central role of glutathione-mediated resistance to cadmium toxicity.","authors":"Shikha Gupta, M Sudhakara Reddy","doi":"10.1007/s00572-025-01244-1","DOIUrl":"10.1007/s00572-025-01244-1","url":null,"abstract":"<p><p>Ectomycorrhizal (ECM) fungi play a vital role in the bioremediation of heavy metal contaminated soil and protecting the host plants from metal stress. In this study, we employed a comparative proteomic approach to investigate the molecular response of ECM fungus Laccaria bicolor to cadmium (Cd) stress. Out of total 997 proteins identified, 154 proteins with a fold change ≥ 1.5 and p < 0.05 were classified as differentially abundant proteins (DAPs) and selected for analysis. KEGG-based functional annotation revealed that Cd exposure disrupted key metabolic pathways including carbohydrate, nucleotide and energy metabolism, thereby inducing cellular energy stress. Proteins involved in genetic information processing, such as DNA replication, repair, transcription, translation, and protein folding, were significantly downregulated, indicating genomic instability and impaired protein quality control. Furthermore, Cd stress affected cellular homeostasis by altering membrane transport and vesicular trafficking systems. In response, L. bicolor activated multiple defense mechanisms to counteract the Cd toxicity, notably upregulating the proteins involved in oxidative stress mitigation, particularly those associated with glutathione metabolism, as well as MAPK and calcium signaling pathways. The consistent upregulation of glutathione and many other related enzymes highlight their central role in Cd detoxification. Overall, this study provides comprehensive insights into the molecular strategies deployed by L. bicolor for Cd tolerance, identifying potential biomarkers and target genes for future biotechnological applications in phytoremediation and stress resilience. Also, this study highlights the active role of glutathione biosynthesis and metabolism proteins in Cd stress mitigation.</p>","PeriodicalId":18965,"journal":{"name":"Mycorrhiza","volume":"35 6","pages":"70"},"PeriodicalIF":3.8,"publicationDate":"2025-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145708607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Boosting coffee seedling performance through arbuscular mycorrhizal association.","authors":"Carlos C Gomes Júnior, Raylla P B de Souza, Karen M S Menezes, Antônio H de Souza, Angélica Tomazeli-Silva, Samuel V Valadares, Samuel C V Martins, Amanda A Cardoso, Marliane C S Da Silva, Fábio M DaMatta","doi":"10.1007/s00572-025-01245-0","DOIUrl":"10.1007/s00572-025-01245-0","url":null,"abstract":"<p><p>We evaluated the effects of arbuscular mycorrhizal fungi (AMF) inoculation on growth, root system architecture, and photosynthetic performance of Coffea arabica seedlings. In a greenhouse experiment using unsterilized soil, seedlings were grown either with (+ M) or without (-M) the addition of AMF inoculum. +M plants exhibited higher net CO₂ assimilation rates and maximum carboxylation capacity of RuBisCO despite reduced stomatal conductance (and transpiration rates), resulting in improved water-use efficiency. These physiological adjustments were associated with greater photochemical utilization of incident light. In addition, +M plants showed increased foliar phosphorus concentration and shifts in leaf metabolic profiles, characterized by higher starch and total free amino acids, reduced hexose sugars, and unchanged sucrose and protein concentrations. Compared with -M plants, +M seedlings displayed pronounced modifications in root system architecture, including greater total root length, surface area, and volume, with a higher proportion of fine roots, while biomass partitioning remained unchanged. Collectively, these morphological and physiological responses resulted in superior vegetative growth in + M plants. AMF inoculation thus represents a promising approach to produce more vigorous and stress-resilient coffee seedlings, potentially facilitating field establishment and reducing production costs.</p>","PeriodicalId":18965,"journal":{"name":"Mycorrhiza","volume":"35 6","pages":"69"},"PeriodicalIF":3.8,"publicationDate":"2025-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145687779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phylogenetic clustering and ecological interactions of arbuscular mycorrhizal fungi and their associated microbiome of a spontaneous plant across Moroccan drylands.","authors":"Sulaimon Basiru, Jean Legeay, Soon-Jae Lee, Zahraddeen Kabir Sani, Abdelhadi Ziami, Safaa Machraoui, Khaoula Errafii, Mohamed Hijri","doi":"10.1007/s00572-025-01243-2","DOIUrl":"10.1007/s00572-025-01243-2","url":null,"abstract":"<p><p>Arbuscular mycorrhizal fungi (AMF) are ubiquitous in arid ecosystems, yet their distribution and community structure along spatial and ecological gradients remains insufficiently explored at regional scales. Here, we employed Malva sylvestris L., a native spontaneous plant species, to investigate the distribution patterns, phylogenetic structure, and community interactions of AMF and the associated root microbiome in dryland ecosystems. Sampling was conducted along a 700 km transect extending from the Atlantic coast to inland Morocco, encompassing predominantly semi-arid ecosystems. Amplicon sequencing of the LSU rDNA region of roots and soil samples revealed a highly diverse AMF assemblage spanning ten families, including Domikaceae, Diversisporaceae, Entrophosporaceae, Sclerocystaceae, and Septoglomeraceae, while the most frequent taxa belonged to the genera Dominikia, Entrophospora, Funneliformis, and Rhizophagus. Phylogenetic alpha diversity declined with increasing soil phosphorus (P) and nitrogen (N) but increased with soil potassium, precipitation, and distance from the coastline. AMF community dissimilarity in the rhizosphere was primarily explained by distance from the coastline, MAT, and precipitation together with soil P, N, whereas AMF communities in roots were mainly structured by soil P, N, and carbon. Community assembly processes among root-associated AMF were mainly shaped by total soil N and P: total N drove local AMF community structure (positive Nearest Taxon Index) while variation in soil P increased community turnover among locations (positive beta Nearest Taxon Index). Consequently, network topology was negatively correlated with soil P, and temperature, but positively with precipitation. Specialized AMF taxa, particularly Septoglomus and Funneliformis, acted as hubs in the root fungal network, whereas generalists such as Rhizophagus and Entrophospora drove cross-kingdom associations, interacting strongly with Rhizobium, Sphingomonas, and Caulobacter. Overall, this study advances our understanding of AMF ecology in dryland ecosystems and introduces an innovative bioinformatic workflow that provides new opportunities for exploring mycorrhizal diversity and functions.</p>","PeriodicalId":18965,"journal":{"name":"Mycorrhiza","volume":"35 6","pages":"68"},"PeriodicalIF":3.8,"publicationDate":"2025-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145605079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of spent mushroom compost enhances wheat yield but reduces mycorrhizal associations and grain nutrient concentration.","authors":"Joseph E Carrara, Andrew H Smith, Wade P Heller","doi":"10.1007/s00572-025-01235-2","DOIUrl":"10.1007/s00572-025-01235-2","url":null,"abstract":"<p><p>Developing management practices that enhance crop yield while maintaining soil health is the foremost objective of the regenerative agriculture movement. One avenue to achieving this goal is using biofertilizers and alternative soil amendments to supplement or replace agrochemicals. Here we report the results of a pairwise field trial of spring wheat (Triticum aestivum) wherein we investigated individual and combined impacts of inoculation with arbuscular mycorrhizal fungi (AMF) and a spent mushroom compost amendment (herein mushroom compost). The symbiotic relationship between AMF and plants has been demonstrated to benefit the yield and nutritional quality of many crops by enhancing access to mineral nutrients and water. Mushroom compost, consisting of the devitalized residual substrate following harvest of edible mushrooms, is a byproduct of the mushroom industry and is comprised of a variety of nutrient-rich organic material inputs. Therefore, the objective of this study was to (1) determine the effect to which AMF and mushroom compost individually impact wheat yield and nutritional quality, and (2) examine if these effects are synergistic or antagonistic when both amendments are applied together. We found that mushroom compost addition, regardless of AMF inoculation, enhanced grain yield by ~ 40%, but reduced AMF root colonization level by ~ 25-40%. Additionally, despite yield increases, mushroom compost addition reduced grain phosphorus (P), potassium (K), and magnesium (Mg) concentrations by ~ 10% and boron concentration by ~ 45%. In fact, grain P, K, and Mg concentrations were all correlated with mycorrhizal colonization level. These results suggest that while spent mushroom compost additions enhanced grain yield, this may have led to a mineral nutrient 'dilution effect' exacerbated by negative impacts on AMF colonization and community composition.</p>","PeriodicalId":18965,"journal":{"name":"Mycorrhiza","volume":"35 6","pages":"67"},"PeriodicalIF":3.8,"publicationDate":"2025-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145605068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hyphosphere interactions: P-solubilizing fungi modulate AMF phosphatase activity and mycorrhizal symbiosis via exudate-mediated communication.","authors":"Ivana F Della Mónica, Alicia M Godeas, J Martín Scervino","doi":"10.1007/s00572-025-01242-3","DOIUrl":"10.1007/s00572-025-01242-3","url":null,"abstract":"<p><p>Arbuscular mycorrhizal fungi (AMF) form symbiotic associations with plant roots, enhancing water and nutrient absorption. Phosphate-solubilizing fungi (PSF) can solubilize and mineralize phosphorus, an essential nutrient with low bioavailability, and eventually interact with AMF. However, the understanding of how they interact in the hyphosphere, where root influence is absent, remains limited. Furthermore, the effect of PSF on the phosphatase activity of AMF, related to the P efficiency in acquisition and utilization, within the hyphosphere and mycorrhizosphere zones, remains unclear. Therefore, this study aimed to assess the effect of three different PSF (Talaromyces flavus, T. helicus, and T. diversus) exudates on extracellular acid phosphatases and alkaline phosphatases associated with intra- and extraradical AMF structures in the hyphosphere and mycorrhizosphere, in vitro. To achieve this aim, the AMF Rhizophagus intraradices was cultured with Ri T-DNA transformed carrot roots in a system using Petri dishes that mimicked the hyphosphere (with 2 sections: (a) with roots and AMF, and (b) with only AMF) and the mycorrhizosphere (with roots and AMF in the same place). Different concentrations of PSF exudates were placed in either the hyphosphere or the mycorrhizosphere, and at the end of the experiment (8 weeks), the phosphatase activity of the AMF was measured. This research highlights that the enzymatic activity of AMF is modulated by PSF exudates, depending on whether these exudates are present in the hyphosphere or the mycorrhizosphere. Exudates in the hyphosphere, where PSF are directly associated with AMF hyphae, have a more pronounced effect on AMF extraradical alkaline phosphatases than acid phosphatases, and promote symbiosis efficiency. In contrast, PSF exudates in the mycorrhizosphere had a neutral or negative effect on symbiosis efficiency, improving the extraradical alkaline phosphatases of AMF and the acid phosphatases of the roots. Also, the effect depends on the fungal identity. AMF act as mediators in this context, improving communication between the roots and the hyphosphere microbiome. When exploring the soil, the hyphae encounter compounds produced by microorganisms, thus establishing a complex network of interactions. These interactions enhance the symbiotic efficiency of AMF, modulating the host plant without direct contact. These results show that microbial interactions not only influence the efficiency of phosphorus transfer to plants but also have broader implications for soil health and fertility management.</p>","PeriodicalId":18965,"journal":{"name":"Mycorrhiza","volume":"35 6","pages":"66"},"PeriodicalIF":3.8,"publicationDate":"2025-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145541485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High diversity and variability of root-associated fungi in Aucoumea klaineana, a monodominant Central African timber species.","authors":"Quentin Guidosse, Mélanie Roy, Sébastien Massart, Jean-Louis Doucet, Ludivine Lassois, Caroline De Clerck","doi":"10.1007/s00572-025-01239-y","DOIUrl":"10.1007/s00572-025-01239-y","url":null,"abstract":"<p><p>Aucoumea klaineana is the most important timber species in Central Africa, naturally forming monodominant stands. While soil fungi are crucial for plant growth, their role in promoting monodominance or supporting suppressed, light-demanding trees remains underexplored. This study, the first to analyze the root mycobiota of A. klaineana, investigates fungal communities in monodominant stands and old-growth mixed forests in Gabon, and plantations in the DRC, sampling both canopy-reaching vs. suppressed individuals, using ITS2 rDNA and 18S rDNA high-throughput sequencing. We identified high fungal diversity in both regions but found no \"core mycobiota\" across stand types or tree social status (canopy-reaching vs. suppressed). Fungal communities varied significantly between stand types, emphasizing a context-dependent nature. Moreover, no distinct fungal communities characterize the mycobiota of suppressed trees. Our findings indicate that fungal associations, including mycorrhizal ones, are unlikely to be a driver of monodominance in A. klaineana. These results highlight the need to consider alternative processes, not related to fungal interactions, such as root grafting, in explaining the persistence of suppressed trees and the dynamics of monodominant stands of A. klaineana. Finally, this study illustrates the highly variable and diverse belowground communities associated with A. klaineana, whose functions and interactions could contribute to the sustainable management of this major timber tree species.</p>","PeriodicalId":18965,"journal":{"name":"Mycorrhiza","volume":"35 6","pages":"65"},"PeriodicalIF":3.8,"publicationDate":"2025-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145505709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}