Antoine Sportès, Mathilde Hériché, Damien Inès, Valérie Monfort-Pimet, Claire Rosnoblet, Sophie Trouvelot, Daniel Wipf, Pierre Emmanuel Courty
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引用次数: 0
摘要
营养交换是丛枝菌根(AM)共生的一个关键特征。葡萄藤(Vitis vinifera)是世界上最重要的经济作物之一,其生长和发育严重依赖AM共生。自从根瘤蚜危机以来,栽培葡萄藤是通过将葡萄接穗嫁接到砧木上获得的。在三种不同磷水平下,研究了蒙彼利埃绿伞(Riparia Gloire de Montpellier)砧木对菌根定植的响应。我们通过比较AM真菌Rhizophagus irregularis DAOM197198定殖和非定殖根的转录组分析,探讨了植物磷营养的调控方面。我们已经表明,磷有效性显著影响AM真菌和葡萄的基因表达。我们的转录组学研究揭示了多年生木本植物AM共生过程中普遍存在的分子机制,其中可用P影响了几种功能类别的蛋白质。在不规则龙骨基因组中,编码Pht1转运体的9个基因在高p处理(RiPT8和RiPT11)下下调(RiPT1和RiPT2)或上调(RiPT8和RiPT11),在低p处理(RiPT5)下上调(RiPT9和RiPT10),并以p剂量依赖性方式调节(RiPT9和RiPT10)。am诱导的3种Pht1中有2种(VvPT4和VvPT8)的表达在菌根条件下增强,但在P处理下略有调节。为了免疫VvPT4和VvPT8,我们开发了一种专门为木本植物设计的创新的清根方案。这一技术进步使得仅观察成熟丛枝周围膜上的VvPT4成为可能,其表达受P有效性差异的强烈影响。
A transcriptomic perspective of P trade in mycorrhizal grapevine.
Nutrient exchanges are a key feature of arbuscular mycorrhizal (AM) symbiosis. Grapevine (Vitis vinifera), one of the most economically important crops worldwide, relies heavily on AM symbiosis for its growth and development. Since the phylloxera crisis, cultivated grapevines are obtained by grafting a Vitis vinifera scion onto a rootstock. In this study, we investigated the responses of the rootstock "Riparia Gloire de Montpellier" to mycorrhizal root colonization under three distinct phosphate (P) levels. We explored regulatory aspects of plant P nutrition by comparing the transcriptome profiling of non-colonized roots and roots colonized by the AM fungus Rhizophagus irregularis DAOM197198. We have shown that P availability significantly influences gene expression in both the AM fungus and the grapevine. Our transcriptomic study shed light on the molecular mechanisms that prevail during the AM symbiosis of a perennial woody plant species, with available P affecting several functional classes of proteins. The nine genes coding for Pht1 transporters in the R. irregularis genome were either down-regulated (RiPT1 and RiPT2) or up-regulated by the high-P treatment (RiPT8 and RiPT11), up-regulated by the low-P treatment (RiPT5), and regulated in a P-dose-dependent manner (RiPT9 and RiPT10). Expression of two of the three identified AM-induced Pht1, VvPT4 and VvPT8, was enhanced under mycorrhizal conditions, but finely tuned by the P treatment. To immunolocalize VvPT4 and VvPT8, we developed an innovative root-clearing protocol specifically designed for woody plants. This technological advancement has made it possible to visualize only VvPT4 at the periarbuscular membrane of mature arbuscules, its expression being strongly influenced by differences in P availability.
期刊介绍:
Mycorrhiza is an international journal devoted to research into mycorrhizas - the widest symbioses in nature, involving plants and a range of soil fungi world-wide. The scope of Mycorrhiza covers all aspects of research into mycorrhizas, including molecular biology of the plants and fungi, fungal systematics, development and structure of mycorrhizas, and effects on plant physiology, productivity, reproduction and disease resistance. The scope also includes interactions between mycorrhizal fungi and other soil organisms and effects of mycorrhizas on plant biodiversity and ecosystem structure.
Mycorrhiza contains original papers, short notes and review articles, along with commentaries and news items. It forms a platform for new concepts and discussions, and is a basis for a truly international forum of mycorrhizologists from all over the world.