Integrative transcriptomic and physiological analyses uncover mechanisms by which arbuscular mycorrhizal fungi mitigate salt stress in sugar beet.

IF 3.8 2区 生物学 Q2 MYCOLOGY
Zeyuan Cui, Xiaodong Li, Pingan Han, Rui Chen, Yinzhuang Dong, Gui Geng, Lihua Yu, Jiahui Liu, Yao Xu, Yuguang Wang
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Abstract

Sugar beet (Beta vulgaris L.) is cultivated extensively worldwide as an important cash crop, and soil salinity is a critical factor influencing both its yield and sugar content. Consequently, enhancing the salt tolerance of sugar beet is of paramount importance. Arbuscular mycorrhizal (AM) fungi form symbiotic associations with approximately 80% of vascular plants, thereby improving the adaptability of host plants to adverse conditions. However, the mechanisms by which the AM symbiosis assists sugar beet in coping with salt stress remain poorly understood. To investigate the adaptation strategies employed by AM symbiotic sugar beet under salt stress, we examined physiological and transcriptomic changes in sugar beet seedlings subjected to various treatments, using the KWS1176 variety as the experimental material. The results indicated that AM symbiotic sugar beet demonstrated superior performance under salt stress, characterized by improved seedling growth, alterations in antioxidant enzyme activities, modifications in osmoregulatory substance levels, reduced Na+ uptake, and enhanced K+ influx within the root system. Notably, most of the differentially expressed genes were implicated in pathways related to reactive oxygen species scavenging, phenylpropanoid biosynthesis, and phytohormone signal transduction. Furthermore, pivotal genes identified through weighted gene co-expression network analysis were validated via reverse transcription-quantitative PCR, revealing that the salt tolerance of AM symbiotic sugar beet may be associated with its ionic homeostasis, antioxidant enzyme activities, and regulation of photosynthesis at both transcriptional and physiological levels.

综合转录组学和生理学分析揭示了丛枝菌根真菌减轻甜菜盐胁迫的机制。
甜菜(Beta vulgaris L.)作为一种重要的经济作物在世界范围内广泛种植,土壤盐分是影响其产量和糖含量的关键因素。因此,提高甜菜的耐盐性是至关重要的。丛枝菌根(AM)真菌与大约80%的维管植物形成共生关系,从而提高寄主植物对不利条件的适应性。然而,AM共生帮助甜菜应对盐胁迫的机制仍然知之甚少。为了研究AM共生甜菜在盐胁迫下的适应策略,我们以KWS1176品种为实验材料,研究了不同处理下甜菜幼苗的生理和转录组变化。结果表明,AM共生甜菜在盐胁迫下表现出优异的生长性能,表现为幼苗生长改善,抗氧化酶活性改变,渗透调节物质水平改变,根系Na+吸收减少,K+内流增加。值得注意的是,大多数差异表达基因与活性氧清除、苯丙素生物合成和植物激素信号转导相关的途径有关。此外,通过加权基因共表达网络分析鉴定的关键基因通过逆转录-定量PCR验证,揭示AM共生甜菜的耐盐性可能在转录和生理水平上与其离子稳态、抗氧化酶活性和光合作用调控有关。
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来源期刊
Mycorrhiza
Mycorrhiza 生物-真菌学
CiteScore
8.20
自引率
2.60%
发文量
40
审稿时长
6-12 weeks
期刊介绍: Mycorrhiza is an international journal devoted to research into mycorrhizas - the widest symbioses in nature, involving plants and a range of soil fungi world-wide. The scope of Mycorrhiza covers all aspects of research into mycorrhizas, including molecular biology of the plants and fungi, fungal systematics, development and structure of mycorrhizas, and effects on plant physiology, productivity, reproduction and disease resistance. The scope also includes interactions between mycorrhizal fungi and other soil organisms and effects of mycorrhizas on plant biodiversity and ecosystem structure. Mycorrhiza contains original papers, short notes and review articles, along with commentaries and news items. It forms a platform for new concepts and discussions, and is a basis for a truly international forum of mycorrhizologists from all over the world.
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