Yung-I Lee, Franziska E Zahn, Qiao-Yi Xie, Shu-Hui Wu, Gerhard Gebauer
{"title":"双花兰根菌根的多样性及其营养。","authors":"Yung-I Lee, Franziska E Zahn, Qiao-Yi Xie, Shu-Hui Wu, Gerhard Gebauer","doi":"10.1007/s00572-025-01208-5","DOIUrl":null,"url":null,"abstract":"<p><p>Fully mycoheterotrophic (FMH) orchids rely entirely on mycorrhizal fungi for carbon and nutrients, with tropical Asian FMH orchids typically associating with saprotrophic fungi, though some known relationships also with ectomycorrhizal fungi, leaving much to learn about their fungal partners. Didymoplexis belongs to tribe Gastrodieae, which represents one of the largest fully mycoheterotrophic orchid lineages. Although mycorrhizal associations of its sister genus Gastrodia have been relatively well-studied, those of Didymoplexis remain largely unexplored. Here, we used molecular barcoding to analyze fungal associations and stable isotope analysis to elucidate the nutritional strategies of Didymoplexis micradenia, Didymoplexis pallens, and Didymoplexis siamensis in subtropical and tropical forests across Taiwan. In Didymoplexis pallens and Didymoplexis micradenia, most fungal partners were litter-decaying fungi (Mycena, Clitocybula, Marasmius, Gymnopus) with smaller contributions from ectomycorrhizal and rhizoctonia fungi. In Didymoplexis siamensis, ectomycorrhizal fungi dominated, particularly Sebacinales, however, with additional associations with wood-decaying Delicatula. The pattern of carbon and nitrogen isotope enrichments found for the three Didymoplexis species was in the typical range known for fully mycoheterotrophic orchids associated with litter- or wood-decaying fungi. <sup>15</sup>N enrichments of all investigated Didymoplexis species distinguished from fully mycoheterotrophic orchids associated with ectomycorrhizal fungi. Despite its ectomycorrhizal association, Didymoplexis siamensis was weakly enriched in <sup>15</sup>N and more enriched in <sup>13</sup>C than found for exclusively ectomycorrhizal fully mycoheterotrophic orchids. Thus, Didymoplexis siamensis covered its carbon and nitrogen demand obviously through the additional association with wood-decaying Delicatula. These findings enhance our understanding of the diverse fungal associations and physiological ecology of Didymoplexis species in subtropical and tropical ecosystems.</p>","PeriodicalId":18965,"journal":{"name":"Mycorrhiza","volume":"35 3","pages":"34"},"PeriodicalIF":3.8000,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Diverse mycorrhizal associations and nutrition in Didymoplexis orchids.\",\"authors\":\"Yung-I Lee, Franziska E Zahn, Qiao-Yi Xie, Shu-Hui Wu, Gerhard Gebauer\",\"doi\":\"10.1007/s00572-025-01208-5\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Fully mycoheterotrophic (FMH) orchids rely entirely on mycorrhizal fungi for carbon and nutrients, with tropical Asian FMH orchids typically associating with saprotrophic fungi, though some known relationships also with ectomycorrhizal fungi, leaving much to learn about their fungal partners. Didymoplexis belongs to tribe Gastrodieae, which represents one of the largest fully mycoheterotrophic orchid lineages. Although mycorrhizal associations of its sister genus Gastrodia have been relatively well-studied, those of Didymoplexis remain largely unexplored. Here, we used molecular barcoding to analyze fungal associations and stable isotope analysis to elucidate the nutritional strategies of Didymoplexis micradenia, Didymoplexis pallens, and Didymoplexis siamensis in subtropical and tropical forests across Taiwan. In Didymoplexis pallens and Didymoplexis micradenia, most fungal partners were litter-decaying fungi (Mycena, Clitocybula, Marasmius, Gymnopus) with smaller contributions from ectomycorrhizal and rhizoctonia fungi. In Didymoplexis siamensis, ectomycorrhizal fungi dominated, particularly Sebacinales, however, with additional associations with wood-decaying Delicatula. The pattern of carbon and nitrogen isotope enrichments found for the three Didymoplexis species was in the typical range known for fully mycoheterotrophic orchids associated with litter- or wood-decaying fungi. <sup>15</sup>N enrichments of all investigated Didymoplexis species distinguished from fully mycoheterotrophic orchids associated with ectomycorrhizal fungi. Despite its ectomycorrhizal association, Didymoplexis siamensis was weakly enriched in <sup>15</sup>N and more enriched in <sup>13</sup>C than found for exclusively ectomycorrhizal fully mycoheterotrophic orchids. Thus, Didymoplexis siamensis covered its carbon and nitrogen demand obviously through the additional association with wood-decaying Delicatula. These findings enhance our understanding of the diverse fungal associations and physiological ecology of Didymoplexis species in subtropical and tropical ecosystems.</p>\",\"PeriodicalId\":18965,\"journal\":{\"name\":\"Mycorrhiza\",\"volume\":\"35 3\",\"pages\":\"34\"},\"PeriodicalIF\":3.8000,\"publicationDate\":\"2025-04-26\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Mycorrhiza\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1007/s00572-025-01208-5\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"MYCOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Mycorrhiza","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s00572-025-01208-5","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MYCOLOGY","Score":null,"Total":0}
Diverse mycorrhizal associations and nutrition in Didymoplexis orchids.
Fully mycoheterotrophic (FMH) orchids rely entirely on mycorrhizal fungi for carbon and nutrients, with tropical Asian FMH orchids typically associating with saprotrophic fungi, though some known relationships also with ectomycorrhizal fungi, leaving much to learn about their fungal partners. Didymoplexis belongs to tribe Gastrodieae, which represents one of the largest fully mycoheterotrophic orchid lineages. Although mycorrhizal associations of its sister genus Gastrodia have been relatively well-studied, those of Didymoplexis remain largely unexplored. Here, we used molecular barcoding to analyze fungal associations and stable isotope analysis to elucidate the nutritional strategies of Didymoplexis micradenia, Didymoplexis pallens, and Didymoplexis siamensis in subtropical and tropical forests across Taiwan. In Didymoplexis pallens and Didymoplexis micradenia, most fungal partners were litter-decaying fungi (Mycena, Clitocybula, Marasmius, Gymnopus) with smaller contributions from ectomycorrhizal and rhizoctonia fungi. In Didymoplexis siamensis, ectomycorrhizal fungi dominated, particularly Sebacinales, however, with additional associations with wood-decaying Delicatula. The pattern of carbon and nitrogen isotope enrichments found for the three Didymoplexis species was in the typical range known for fully mycoheterotrophic orchids associated with litter- or wood-decaying fungi. 15N enrichments of all investigated Didymoplexis species distinguished from fully mycoheterotrophic orchids associated with ectomycorrhizal fungi. Despite its ectomycorrhizal association, Didymoplexis siamensis was weakly enriched in 15N and more enriched in 13C than found for exclusively ectomycorrhizal fully mycoheterotrophic orchids. Thus, Didymoplexis siamensis covered its carbon and nitrogen demand obviously through the additional association with wood-decaying Delicatula. These findings enhance our understanding of the diverse fungal associations and physiological ecology of Didymoplexis species in subtropical and tropical ecosystems.
期刊介绍:
Mycorrhiza is an international journal devoted to research into mycorrhizas - the widest symbioses in nature, involving plants and a range of soil fungi world-wide. The scope of Mycorrhiza covers all aspects of research into mycorrhizas, including molecular biology of the plants and fungi, fungal systematics, development and structure of mycorrhizas, and effects on plant physiology, productivity, reproduction and disease resistance. The scope also includes interactions between mycorrhizal fungi and other soil organisms and effects of mycorrhizas on plant biodiversity and ecosystem structure.
Mycorrhiza contains original papers, short notes and review articles, along with commentaries and news items. It forms a platform for new concepts and discussions, and is a basis for a truly international forum of mycorrhizologists from all over the world.