Mycorrhiza最新文献

{"title":"Mycelial communities associated with Ostrya carpinifolia, Quercus pubescens and Pinus nigra in a patchy Sub-Mediterranean Karst woodland.","authors":"Tanja Mrak, Philip Alan Brailey-Crane, Nataša Šibanc, Tijana Martinović, Jožica Gričar, Hojka Kraigher","doi":"10.1007/s00572-025-01220-9","DOIUrl":"10.1007/s00572-025-01220-9","url":null,"abstract":"<p><p>Common mycorrhizal networks of ectomycorrhizal (EcM) fungi could be of great benefit to trees growing in the shallow soils of Sub-Mediterranean Karst ecosystems, potentially playing a crucial role in the survival of trees in this harsh environment. The first step to confirm the existence of such networks is to assess the extent and nature of symbiont sharing in the mycelial community. To address this question, we incubated in-growth mesh bags under the native Ostrya carpinifolia and Quercus pubescens, and the non-native Pinus nigra, over two consecutive years. In Q. pubescens and P. nigra, but not in O. carpinifolia, mycelium production was significantly higher in the year with higher spring precipitation, indicating the influence of climatic conditions, but also the identity of the host tree. We observed a complex interaction between tree species and sampling year in structuring the composition and diversity of mycelial communities. Local environmental conditions contributed additionally and were responsible for 21.46% of the community variation between samples. Although ~ 70% of fungal operational taxonomic units were shared across the studied tree species, distinct community compositions emerged, emphasizing the role of host tree specificity. Q. pubescens exhibited greater stability in EcM richness between sampling years, whereas P. nigra showed lower EcM richness, likely due to limited availability of compatible fungi and reliance on introduced fungal partners. Additionally, differences in EcM fungal exploration strategies were observed. O. carpinifolia and Q. pubescens mainly hosted non-specific EcM fungi with short distance exploration types. In contrast, EcM fungi of P. nigra had higher spatial spread, and were predominantly conifer specific. Overall, our results emphasize the importance of host specificity, soil parameters, spatial proximity, and climatic variability for the structuring of mycelial communities in fragmented forests.</p>","PeriodicalId":18965,"journal":{"name":"Mycorrhiza","volume":"35 4","pages":"46"},"PeriodicalIF":3.8,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12296906/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144708221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Accumulation of glomalin-related soil protein to soil carbon storage in forest ecosystems along an elevation gradient.","authors":"Nuttaporn Luyprasert, Palingamoorthy Gnanamoorthy, Shangwen Xia, Ashutosh Kumar Singh, Xiaodong Yang","doi":"10.1007/s00572-025-01219-2","DOIUrl":"10.1007/s00572-025-01219-2","url":null,"abstract":"<p><p>Glomalin-related soil protein (GRSP), a glycoprotein produced by arbuscular mycorrhizal fungi (AMF), plays a critical role in soil organic carbon (SOC) storage in forest ecosystems. However, the factors influencing its variability and contribution to SOC along forest elevation gradients remain poorly understood, limiting our ability to predict how soil carbon sequestration through GRSP will respond to future global warming. Therefore, this study explored the relationships among GRSP and SOC with climatic and edaphic factors along elevation gradients (666-3892 m) spanning diverse forest types in tropical rainforest, subtropical forest, and subalpine forest in Yunnan, Southwest China. Our findings revealed that AMF spore abundance declined, whereas AMF root colonization and GRSP concentrations increased with increasing elevation. GRSP showed a stronger positive correlation with AMF root colonization than with spore abundance, particularly in subtropical and subalpine forests where nutrient availability was higher. A significant positive relationship was observed between GRSP and SOC across all three forest sites, with the strongest association in subtropical forests. These results suggest that GRSP accumulation is sensitive to climate and nutrient cycling, emphasizing the role of AMF activity and GRSP as an AMF-derived compound in mediating SOC storage across elevation gradients, consequently contributing to climate change mitigation.</p>","PeriodicalId":18965,"journal":{"name":"Mycorrhiza","volume":"35 4","pages":"45"},"PeriodicalIF":3.8,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144659658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between a liverwort and arbuscular mycorrhizal fungi: a promising strategy for the phytoremediation of polycyclic aromatic hydrocarbons.","authors":"Romina Storb, Maya Svriz, Elisabet Aranda, Sebastián Fracchia, Nahuel Spinedi, José Martin Scervino","doi":"10.1007/s00572-025-01217-4","DOIUrl":"10.1007/s00572-025-01217-4","url":null,"abstract":"<p><p>Soil contamination with polycyclic aromatic hydrocarbons (PAHs) represents a major environmental challenge and requires cost-effective and environmentally friendly remediation technologies. Phytoremediation, enhanced by arbuscular mycorrhizal fungi (AMF), is an effective and extensive technique for PAHs remediation, although, its application with non-vascular plants, is largely unexplored. This study investigates the role of the AMF Rhizophagus irregularis in the uptake and bioaccumulation of anthracene in the liverwort Lunularia cruciata under in vitro conditions. The thallus and the AMF were able to absorb and bioaccumulate anthracene in the cell wall and spores, hyphae and arbuscules respectively. Our results indicate that the liverwort-fungus system employs multiple phytoremediation mechanisms, including phytoaccumulation and phytostabilization. At intermediate contamination levels, the fungal symbiont enhanced contaminant accumulation in the plant, whereas at higher contamination levels, this effect diminished, suggesting a potential limitation in fungal-mediated uptake under extreme conditions. These findings highlight the potential of AMF symbiosis in liverworts for developing biological tools for PAHs remediation, emphasizing the dependence on pollutant concentration for the effectiveness of phytoremediation.</p>","PeriodicalId":18965,"journal":{"name":"Mycorrhiza","volume":"35 4","pages":"44"},"PeriodicalIF":3.8,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144326293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated peloton and fruiting body isotope data shed light on mycoheterotrophic interactions in Gastrodia pubilabiata (Orchidaceae).","authors":"Kenji Suetsugu, Hidehito Okada","doi":"10.1007/s00572-025-01213-8","DOIUrl":"10.1007/s00572-025-01213-8","url":null,"abstract":"<p><p>The mycoheterotrophic nutritional mode, characterized by the acquisition of fungal-derived carbon by plants, has long captivated botanists and mycologists. Recent stable isotope analyses of fungal pelotons isolated from roots have advanced our understanding of this nutritional strategy; however, concerns remain regarding potential isotopic biases, particularly ¹⁵N depletion during lysis or extraction. To address these concerns, we focused on Gastrodia pubilabiata, a fully mycoheterotrophic orchid that associates with saprotrophic fungi. This species offers an ideal system to test whether peloton tissues reliably reflect fungal isotope signatures, as its mycorrhizal roots occasionally occur in direct contact with the fruiting bodies of its fungal partner. We measured δ¹³C and δ¹⁵N values in the aboveground tissues of G. pubilabiata, pelotons extracted from its roots, and fruiting bodies of the associated wood-decaying fungus Cyanotrama gypsea, along with neighboring autotrophic reference plants. The stable isotope analysis revealed that δ¹³C values were nearly identical between pelotons and fruiting bodies, while δ¹⁵N values were slightly higher in pelotons, indicating that peloton-derived isotopic data reliably reflect the fungal source. Moreover, the ¹³C and ¹⁵N enrichment observed in the orchid relative to the fungal fractions was broadly consistent with expected trophic-level fractionation, suggesting a predator-prey-like mode of nutrient transfer. Taken together, these findings support the validity of recently developed isotope-based approaches using extracted pelotons to represent fungal isotopic signatures, at least within this system.</p>","PeriodicalId":18965,"journal":{"name":"Mycorrhiza","volume":"35 3","pages":"43"},"PeriodicalIF":3.3,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12158851/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144266664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ecological specificity of fungi on seedling establishment in Dendrobium huoshanense: a narrow distributed medicinal orchid.","authors":"Yu Zhang, Shi-Wen Wang, Neng-Qi Li, Qian Jin, Rengasamy Anbazhakan, Ya-Feng Dai, Zeng-Xu Xiang, Jiang-Yun Gao","doi":"10.1007/s00572-025-01216-5","DOIUrl":"10.1007/s00572-025-01216-5","url":null,"abstract":"","PeriodicalId":18965,"journal":{"name":"Mycorrhiza","volume":"35 3","pages":"41"},"PeriodicalIF":3.3,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144248863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual-mycorrhizal colonization is determined by plant age and host identity in two species of Populus.","authors":"Jake Nash, Brian Looney, Melissa A Cregger, Christopher Schadt, Rytas Vilgalys","doi":"10.1007/s00572-025-01215-6","DOIUrl":"10.1007/s00572-025-01215-6","url":null,"abstract":"<p><p>Plants have evolved symbioses with mycorrhizal and endophytic fungi that are essential for their growth and survival. While most plants associate with a single guild of mycorrhizal fungi, a select group termed \"dual-mycorrhizal plants\" associate with both arbuscular mycorrhizal and ectomycorrhizal fungi. Although a shift from predominance of arbuscular mycorrhizal to ectomycorrhizal colonization with plant development has been demonstrated on other dual-mycorrhizal hosts, it is not known how mycorrhizal colonization shifts with plant age in Populus species. We performed a controlled growth experiment with natural field-sourced inocula to test for age-dependent shifts in fungal colonization rates and for host-specific patterns of colonization in two species of Populus (P. tremuloides and P. trichocarpa). We found that only P. trichocarpa displayed dual-mycorrhizal colonization, while P. tremuloides associated with ectomycorrhizal fungi, but not arbuscular mycorrhizal fungi. Both guilds of mycorrhizal fungi increased in abundance with plant age, while root endophytic fungal colonization decreased. Many of the early-colonizing endophytic fungi that we documented have strong saprotrophic capabilities, which may be an important trait for fast colonization. Dark septate endophytes were more abundant than either guild of mycorrhizal fungi, and are likely to be functionally important members of the Populus root fungal community. Our findings represent a novel pattern in the development of dual-mycorrhizal colonization and illustrate that Populus species vary in their association with arbuscular mycorrhizal fungi. Our results also highlight the importance of dark septate endophyte colonization dynamics on dual-mycorrhizal plants.</p>","PeriodicalId":18965,"journal":{"name":"Mycorrhiza","volume":"35 3","pages":"42"},"PeriodicalIF":3.3,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144258612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional traits of Asteraceae species vary with arbuscular mycorrhizal fungal identity and phylogeny.","authors":"Cem Turanoglu, Héloïse Ancel, Cécile Le Lann, Joan Van Baaren, Ophélie Bazin, Stéphane Declerck, Florian Fort, Thierry Fontaine Breton, Philippe Vandenkoornhuyse, Cendrine Mony","doi":"10.1007/s00572-025-01214-7","DOIUrl":"10.1007/s00572-025-01214-7","url":null,"abstract":"<p><p>Plants interact closely with arbuscular mycorrhizal (AM) fungi. They allocate photosynthates to AM fungi in exchange for nutrients, thereby influencing plant fitness. Although plant phenotypes result from multiple traits constrained by trade-offs, the effects of AM fungi on plants are often studied using one trait. Plant response to AM fungi therefore needs to be analysed using multiple traits. Four Asteraceae species were inoculated with ten AM fungal strains using a factorial design in the greenhouse. The effect of AM fungal inoculation and their taxonomic identity on plants vegetative and reproductive traits were assessed. The predictability of the effects was evaluated based on AM fungal phylogenetic relatedness. The effect of the inoculation depended on the trait considered. Compared with the non-inoculated control, biomass allocation to roots decreased, while allocations to shoots or reproduction increased depending on the AM fungi or the plant. Significant differences were observed among AM fungi inoculates used, whether looking at separate traits, trade-offs between vegetative or reproductive growth, and the trait syndrome. In Centaurea cyanus, changes in plant phenotype were associated with the phylogenetic distance between AM fungi. These results underline the importance of using multi-trait approaches to understand AM fungal effects on plant phenotype. In accordance with the holobiont concept, the outcomes of this interaction depended on both the host plant and the AM fungus involved. It contributes to a better understanding of the biological effectors shaping the reaction norm (i.e., the range of phenotypic variation of a given host genotype) within the plant holobiont.</p>","PeriodicalId":18965,"journal":{"name":"Mycorrhiza","volume":"35 3","pages":"40"},"PeriodicalIF":3.3,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144226015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A transcriptomic perspective of P trade in mycorrhizal grapevine.","authors":"Antoine Sportès, Mathilde Hériché, Damien Inès, Valérie Monfort-Pimet, Claire Rosnoblet, Sophie Trouvelot, Daniel Wipf, Pierre Emmanuel Courty","doi":"10.1007/s00572-025-01200-z","DOIUrl":"10.1007/s00572-025-01200-z","url":null,"abstract":"<p><p>Nutrient exchanges are a key feature of arbuscular mycorrhizal (AM) symbiosis. Grapevine (Vitis vinifera), one of the most economically important crops worldwide, relies heavily on AM symbiosis for its growth and development. Since the phylloxera crisis, cultivated grapevines are obtained by grafting a Vitis vinifera scion onto a rootstock. In this study, we investigated the responses of the rootstock \"Riparia Gloire de Montpellier\" to mycorrhizal root colonization under three distinct phosphate (P) levels. We explored regulatory aspects of plant P nutrition by comparing the transcriptome profiling of non-colonized roots and roots colonized by the AM fungus Rhizophagus irregularis DAOM197198. We have shown that P availability significantly influences gene expression in both the AM fungus and the grapevine. Our transcriptomic study shed light on the molecular mechanisms that prevail during the AM symbiosis of a perennial woody plant species, with available P affecting several functional classes of proteins. The nine genes coding for Pht1 transporters in the R. irregularis genome were either down-regulated (RiPT1 and RiPT2) or up-regulated by the high-P treatment (RiPT8 and RiPT11), up-regulated by the low-P treatment (RiPT5), and regulated in a P-dose-dependent manner (RiPT9 and RiPT10). Expression of two of the three identified AM-induced Pht1, VvPT4 and VvPT8, was enhanced under mycorrhizal conditions, but finely tuned by the P treatment. To immunolocalize VvPT4 and VvPT8, we developed an innovative root-clearing protocol specifically designed for woody plants. This technological advancement has made it possible to visualize only VvPT4 at the periarbuscular membrane of mature arbuscules, its expression being strongly influenced by differences in P availability.</p>","PeriodicalId":18965,"journal":{"name":"Mycorrhiza","volume":"35 3","pages":"39"},"PeriodicalIF":3.3,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144160157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a high-throughput spore germination test to assess the toxicity of pesticides on arbuscular mycorrhizal fungi.","authors":"Marjan Roshanfekrrad, Christos Papadopoulos, Maryline Calonne-Salmon, Carolin Schneider, Kunyang Zhang, Dimitrios Karpouzas, Stephan Declerck","doi":"10.1007/s00572-025-01211-w","DOIUrl":"10.1007/s00572-025-01211-w","url":null,"abstract":"<p><p>Pesticides are essential agricultural inputs that help securing crop yields. However, they can affect non-target soil microorganisms, including arbuscular mycorrhizal (AM) fungi, that are potential indicators of the toxicity of pesticides on the soil microbiota. Here, we developed a fast-track high-throughput spore germination test, for AM fungi produced in vitro. This test allows the determination of EC₅₀ values and the nature of the effects of pesticides on AM fungal spores (fungicidal or fungistatic). First, 19 active ingredients were tested on Rhizophagus intraradices MUCL 49410. Secondly, five of these compounds, varying in their toxicity to R. intraradices, were tested on three additional AM fungi (Rhizophagus irregularis MUCL 41833, Rhizophagus clarus MUCL 46238 and Rhizophagus aggregatus MUCL 49408). Our results showed that the toxicity of pesticides varied according to their chemical nature, concentration and AM fungal species tested. With the exception of 3,5,6-trichloro-2-pyridinol (TCP, a transformation product of chlorpyrifos), insecticides and herbicides had no detrimental effect on spore germination at the concentration expected in soil upon application of the recommended dose, unlike most fungicides, which had an impact on one or more AM fungi. Fludioxonil and pyraclostrobin were by far the most problematic fungicide and R. aggregatus the most sensitive strain to pesticides. This AM fungus could thus be a good indicator to be used in standard ecotoxicity testing. In conclusion, we present a fast-track, high-throughput testing system for assessing the toxicity of pesticides on AM fungi, using spore germination as a relevant endpoint, that could be used as a first-tier screening tool in pesticide risk assessment.</p>","PeriodicalId":18965,"journal":{"name":"Mycorrhiza","volume":"35 3","pages":"38"},"PeriodicalIF":3.3,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144079231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The impact of land use change on mycorrhizal fungi and their associations with rodents: insights from a temperate forest in Mexico.","authors":"Margarita Gil-Fernández, Alexandra J R Carthey, Eduardo Mendoza, Oscar Godínez-Gómez, M Cristina MacSwiney G, Arnulfo Blanco-García, Christian A Delfín-Alfonso, Johannes J Le Roux","doi":"10.1007/s00572-025-01210-x","DOIUrl":"10.1007/s00572-025-01210-x","url":null,"abstract":"<p><p>Ecosystem functioning is influenced by biological diversity, ecological interactions, and abiotic conditions. Human interactions with ecosystems can cause major changes in how they function when involving changes in the vegetation cover and structure (i.e., land use change). This study examines how land use change affects the diversity of arbuscular mycorrhizal fungi (AMF) and ectomycorrhizal fungi (EMF) in soil and rodent scats in temperate forest sites. We collected soil and rodent scat samples at five paired sites (i.e., disturbed vs. undisturbed) in Michoacan, Mexico. We identified 112 putative mycorrhizal fungi species using DNA barcoding based on partial internal transcribed region 1 (ITS) sequences. We found a higher richness of EMF in undisturbed soil samples compared to disturbed soil samples and a higher AMF diversity in rodent scat samples from disturbed than undisturbed sites. Scat samples had a high incidence of both AMF (75%) and EMF (100%). We found significant differences in the diversity of both AMF and EMF depending on the rodent species associated with them. We also found a higher diversity of EMF in scats in the wet season than in the dry season. We also report, for the first time, associations between Sigmodon hispidus and numerous AMF and EMF species. Overall, our study highlights the role of rodents as important dispersal vectors of mycorrhizal fungi, particularly for EMF that could be essential to build up mycorrhizal fungi spore banks in disturbed forests.</p>","PeriodicalId":18965,"journal":{"name":"Mycorrhiza","volume":"35 3","pages":"36"},"PeriodicalIF":3.3,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12062193/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144003440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}