综合核素和子实体同位素数据揭示了天麻(兰科)真菌异养相互作用。

IF 3.8 2区 生物学 Q2 MYCOLOGY
Kenji Suetsugu, Hidehito Okada
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引用次数: 0

摘要

真菌异养营养模式,以植物获取真菌衍生碳为特征,长期以来一直吸引着植物学家和真菌学家。最近从根中分离的真菌peloton的稳定同位素分析提高了我们对这种营养策略的理解;然而,人们仍然担心潜在的同位素偏差,特别是在裂解或提取过程中15N的损耗。为了解决这些问题,我们专注于天麻,一种与腐养真菌相关的完全分枝异养兰花。该物种提供了一个理想的系统来测试peloton组织是否可靠地反映真菌同位素特征,因为它的菌根偶尔会与真菌伴侣的子实体直接接触。我们测量了G. pubilabiata地上组织的δ13C和δ15N值,从其根中提取的peloton,以及伴生木材腐烂真菌Cyanotrama gypsea的子实体,以及邻近的自养参考植物。稳定同位素分析表明,岩心与子实体的δ13C值基本一致,而岩心的δ15N值略高,说明岩心同位素数据可靠地反映了真菌来源。此外,在兰花中观察到的相对于真菌组分的13C和15N富集与预期的营养水平分异大致一致,表明存在一种类似捕食者-猎物的营养转移模式。综上所述,这些发现支持了最近开发的基于同位素的方法的有效性,该方法使用提取的peloton来代表真菌同位素特征,至少在该系统中是这样。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Integrated peloton and fruiting body isotope data shed light on mycoheterotrophic interactions in Gastrodia pubilabiata (Orchidaceae).

Integrated peloton and fruiting body isotope data shed light on mycoheterotrophic interactions in Gastrodia pubilabiata (Orchidaceae).

Integrated peloton and fruiting body isotope data shed light on mycoheterotrophic interactions in Gastrodia pubilabiata (Orchidaceae).

The mycoheterotrophic nutritional mode, characterized by the acquisition of fungal-derived carbon by plants, has long captivated botanists and mycologists. Recent stable isotope analyses of fungal pelotons isolated from roots have advanced our understanding of this nutritional strategy; however, concerns remain regarding potential isotopic biases, particularly 15N depletion during lysis or extraction. To address these concerns, we focused on Gastrodia pubilabiata, a fully mycoheterotrophic orchid that associates with saprotrophic fungi. This species offers an ideal system to test whether peloton tissues reliably reflect fungal isotope signatures, as its mycorrhizal roots occasionally occur in direct contact with the fruiting bodies of its fungal partner. We measured δ13C and δ15N values in the aboveground tissues of G. pubilabiata, pelotons extracted from its roots, and fruiting bodies of the associated wood-decaying fungus Cyanotrama gypsea, along with neighboring autotrophic reference plants. The stable isotope analysis revealed that δ13C values were nearly identical between pelotons and fruiting bodies, while δ15N values were slightly higher in pelotons, indicating that peloton-derived isotopic data reliably reflect the fungal source. Moreover, the 13C and 15N enrichment observed in the orchid relative to the fungal fractions was broadly consistent with expected trophic-level fractionation, suggesting a predator-prey-like mode of nutrient transfer. Taken together, these findings support the validity of recently developed isotope-based approaches using extracted pelotons to represent fungal isotopic signatures, at least within this system.

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来源期刊
Mycorrhiza
Mycorrhiza 生物-真菌学
CiteScore
8.20
自引率
2.60%
发文量
40
审稿时长
6-12 weeks
期刊介绍: Mycorrhiza is an international journal devoted to research into mycorrhizas - the widest symbioses in nature, involving plants and a range of soil fungi world-wide. The scope of Mycorrhiza covers all aspects of research into mycorrhizas, including molecular biology of the plants and fungi, fungal systematics, development and structure of mycorrhizas, and effects on plant physiology, productivity, reproduction and disease resistance. The scope also includes interactions between mycorrhizal fungi and other soil organisms and effects of mycorrhizas on plant biodiversity and ecosystem structure. Mycorrhiza contains original papers, short notes and review articles, along with commentaries and news items. It forms a platform for new concepts and discussions, and is a basis for a truly international forum of mycorrhizologists from all over the world.
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