Diverse mycorrhizal associations and nutrition in Didymoplexis orchids.

IF 3.8 2区 生物学 Q2 MYCOLOGY
Yung-I Lee, Franziska E Zahn, Qiao-Yi Xie, Shu-Hui Wu, Gerhard Gebauer
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引用次数: 0

Abstract

Fully mycoheterotrophic (FMH) orchids rely entirely on mycorrhizal fungi for carbon and nutrients, with tropical Asian FMH orchids typically associating with saprotrophic fungi, though some known relationships also with ectomycorrhizal fungi, leaving much to learn about their fungal partners. Didymoplexis belongs to tribe Gastrodieae, which represents one of the largest fully mycoheterotrophic orchid lineages. Although mycorrhizal associations of its sister genus Gastrodia have been relatively well-studied, those of Didymoplexis remain largely unexplored. Here, we used molecular barcoding to analyze fungal associations and stable isotope analysis to elucidate the nutritional strategies of Didymoplexis micradenia, Didymoplexis pallens, and Didymoplexis siamensis in subtropical and tropical forests across Taiwan. In Didymoplexis pallens and Didymoplexis micradenia, most fungal partners were litter-decaying fungi (Mycena, Clitocybula, Marasmius, Gymnopus) with smaller contributions from ectomycorrhizal and rhizoctonia fungi. In Didymoplexis siamensis, ectomycorrhizal fungi dominated, particularly Sebacinales, however, with additional associations with wood-decaying Delicatula. The pattern of carbon and nitrogen isotope enrichments found for the three Didymoplexis species was in the typical range known for fully mycoheterotrophic orchids associated with litter- or wood-decaying fungi. 15N enrichments of all investigated Didymoplexis species distinguished from fully mycoheterotrophic orchids associated with ectomycorrhizal fungi. Despite its ectomycorrhizal association, Didymoplexis siamensis was weakly enriched in 15N and more enriched in 13C than found for exclusively ectomycorrhizal fully mycoheterotrophic orchids. Thus, Didymoplexis siamensis covered its carbon and nitrogen demand obviously through the additional association with wood-decaying Delicatula. These findings enhance our understanding of the diverse fungal associations and physiological ecology of Didymoplexis species in subtropical and tropical ecosystems.

双花兰根菌根的多样性及其营养。
完全分枝异养(FMH)兰花完全依赖菌根真菌提供碳和营养,热带亚洲的FMH兰花通常与腐养真菌有关,尽管一些已知的关系也与外生菌根真菌有关,但对它们的真菌伙伴还有很多需要了解的地方。Didymoplexis属天麻科(Gastrodieae),是最大的完全分枝异养兰花系之一。虽然其姐妹属天麻的菌根关联已经被研究得相对较好,但Didymoplexis的菌根关联在很大程度上仍未被探索。本研究利用分子条形码分析真菌关联,并利用稳定同位素分析阐明台湾亚热带和热带森林中微radenia Didymoplexis、pallens Didymoplexis和siamensis Didymoplexis的营养策略。在淡色双胞丛和微核双胞丛中,大多数真菌伴侣是凋落物腐殖真菌(Mycena、Clitocybula、Marasmius、Gymnopus),外生菌根真菌和根丝胞菌贡献较小。在siamensis中,外生菌根真菌占主导地位,特别是Sebacinales,然而,与木材腐烂的Delicatula有额外的联系。三种双藓属植物的碳和氮同位素富集模式处于与凋落物或木材腐烂真菌相关的完全分枝异养兰花的典型范围内。所有被调查的双藓属植物的15N富集都与与外生菌根真菌有关的完全分枝异养兰花不同。尽管与外生菌根有关联,但siamensis在15N下的富集程度较弱,而在13C上的富集程度高于完全外生菌根的完全分枝异养兰花。因此,siamensis通过与木材腐烂的deliatula的额外关联,明显地掩盖了其碳氮需求。这些发现增加了我们对亚热带和热带生态系统中双藓属真菌多样性和生理生态学的认识。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Mycorrhiza
Mycorrhiza 生物-真菌学
CiteScore
8.20
自引率
2.60%
发文量
40
审稿时长
6-12 weeks
期刊介绍: Mycorrhiza is an international journal devoted to research into mycorrhizas - the widest symbioses in nature, involving plants and a range of soil fungi world-wide. The scope of Mycorrhiza covers all aspects of research into mycorrhizas, including molecular biology of the plants and fungi, fungal systematics, development and structure of mycorrhizas, and effects on plant physiology, productivity, reproduction and disease resistance. The scope also includes interactions between mycorrhizal fungi and other soil organisms and effects of mycorrhizas on plant biodiversity and ecosystem structure. Mycorrhiza contains original papers, short notes and review articles, along with commentaries and news items. It forms a platform for new concepts and discussions, and is a basis for a truly international forum of mycorrhizologists from all over the world.
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