Journal of thermal biology最新文献

{"title":"RSK2 facilitates beige fat formation through thermogenic and glycolytic pathways","authors":"Yan Cai ,&nbsp;Jun-jun Chen ,&nbsp;Lin-fei He ,&nbsp;Yao Xu ,&nbsp;Rui-qi Yang ,&nbsp;Zhi-chun Yang","doi":"10.1016/j.jtherbio.2026.104389","DOIUrl":"10.1016/j.jtherbio.2026.104389","url":null,"abstract":"<div><h3>Objectives</h3><div>Beige adipose tissue offers therapeutic potential for metabolic disorders. Notably, glycolytic beige adipocytes, which can be activated independently of β-adrenergic signaling, may provide unique advantages. This study aimed to investigate the role of p90 ribosomal S6 kinase-2 (RSK2) in both cold-induced and cold-independent glycolytic beige adipocyte formation.</div></div><div><h3>Methods</h3><div>We employed C57BL/6J mice subjected to cold exposure (4 °C) or high-fat diet (HFD) and analyzed adipose tissue for thermogenic and glycolytic markers. In vitro, subcutaneous adipose-derived stem cells (primary preadipocytes) were cultured at 28 °C to mimic cold exposure. RSK2 expression was manipulated via knockdown or overexpression to evaluate its functional role.</div></div><div><h3>Results</h3><div>Cold exposure upregulated uncoupling protein-1 (UCP1) and RSK2 in vivo, while HFD suppressed RSK2, UCP1, enolase 1 (ENO1), and pyruvate kinase isoform M2 (PKM2). In primary preadipocytes, cold exposure induced UCP1 and RSK2 expression and reduced triglyceride accumulation. RSK2 knockdown suppressed UCP1, acetyl-CoA carboxylase (ACC), and hormone-sensitive lipase (HSL) but increased carnitine palmitoyl transferase expression. It also reduced PKM2, ENO1, and lactate production under normothermic conditions, whereas RSK2 overexpression had opposite effects. High-glucose and high-insulin treatment decreased RSK2, glycolytic and thermogenic markers, and insulin signaling, which were reversed by RSK2 overexpression. <strong>Conclusions</strong>: RSK2 plays a dual role in mediating cold-induced and cold-independent glycolytic beige adipocyte formation. It may serve as a promising target for treating obesity and related metabolic disorders.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"136 ","pages":"Article 104389"},"PeriodicalIF":2.9,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146078903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic component of sensitivity to heat stress for fertility traits of Jersey crossbred cattle","authors":"Indrajit Gayari ,&nbsp;Sylvia Lalhmingmawii ,&nbsp;Lalmalsawmi Colney ,&nbsp;Hasan Baneh ,&nbsp;Ajoy Mandal","doi":"10.1016/j.jtherbio.2026.104376","DOIUrl":"10.1016/j.jtherbio.2026.104376","url":null,"abstract":"<div><div>Heat stress (HS) attributed by climate change, is a significant challenge to livestock production globally, particularly affecting dairy cows' fertility. This not only leads to decreased reproductive performance but threatens the herd profitability. The aim of this study was to analyze the effect of heat stress on non-return rate (NR56) and conception rate (CR) in Jersey × <em>Bos indicus</em> crossbred cows, to identify the threshold at which these traits are affected, and to estimate genetic parameters and variance components under HS conditions. The study analyzed insemination records of 1174 crossbreds over a period of 26 years, with the use of temperature-humidity index (THI) as an indicator to measure HS. Logistic regression models and Bayesian statistics were employed to identify THI breakpoints (BP) for NR56 and CR. Genetic parameters were estimated using reaction norm models and Gibbs sampling approach for evaluation of variance components. THI breakpoints were identified at 77 for NR56 and 68 for CR. The heritability of NR56 decreased as THI increased, while CR heritability exhibited a peak at higher THI levels. Genetic correlations indicated antagonistic relationships between general additive and HS-specific additive effects for both traits, with increasing additive and environmental variance as THI rose. Thus, this study highlights the genetic diversity associated with heat tolerance in crossbred cows and suggests that selecting for genetically heat-tolerant animals may mitigate HS related fertility declines. Further, these findings provide insight into breeding strategies that can be implemented to enhance reproductive performance of crossbred dairy cow in tropical climatic conditions.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"136 ","pages":"Article 104376"},"PeriodicalIF":2.9,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145980961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sex-dependent calcium dynamics in mouse skeletal muscle: Responses to cooling and caffeine","authors":"Kosei Hayakawa ,&nbsp;Ryo Takagi ,&nbsp;Ayaka Tabuchi ,&nbsp;Haruka Ugawa ,&nbsp;Daiki Watanabe ,&nbsp;Daisuke Hoshino ,&nbsp;David C. Poole ,&nbsp;Yutaka Kano","doi":"10.1016/j.jtherbio.2026.104412","DOIUrl":"10.1016/j.jtherbio.2026.104412","url":null,"abstract":"<div><div>Intracellular calcium ion concentration ([Ca²⁺]i) regulation in skeletal muscle may vary with sex and muscle fiber type, but the precise nature of its response to temperature changes and pharmacological caffeine stimulation is not fully understood. This study aimed to elucidate sex-dependent and muscle fiber type-specific characteristics of muscle cooling, caffeine stimulation, and their combined effects. We investigated the effects of cooling (30 °C to 0 °C) and caffeine stimulation (1.25–80 mM) separately and in combination (cooling + 1.25 mM caffeine) in fast-twitch (plantaris, PLA) and slow-twitch (soleus, SOL) muscles of male and female C57BL/6J mice. [Ca²⁺]i dynamics were analyzed using <em>in vivo</em> Fura-2 bioimaging under isoflurane anesthesia. The temperature threshold for the onset of [Ca²⁺]i accumulation was significantly higher in SOL than in PLA, with no significant difference between sexes (males: PLA 2.3 ± 0.9 °C, SOL 4.5 ± 2.2 °C; females: PLA 2.3 ± 0.8 °C, SOL 4.3 ± 1.3 °C). Conversely, the [Ca²⁺]i response to caffeine was significantly higher in females than in males at high concentrations (80 mM). Furthermore, the combined stimulation of cooling and caffeine had a greater effect on females than on males. Our findings also indicate that the phosphorylation response of ryanodine receptors to caffeine was significantly higher in females than in males. In conclusion, while no sex differences were observed in the [Ca²⁺]i response to cooling, clear sex-dependent differences (males &lt; females) were observed in the response to caffeine.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"136 ","pages":"Article 104412"},"PeriodicalIF":2.9,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146170257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heat waves and health crises: The unseen threat of heat stress on multiple organ systems","authors":"Priya Jaswal ,&nbsp;Dhrubalochan Rana ,&nbsp;Rishabh Chaudhary ,&nbsp;Jhilli Basu ,&nbsp;Nitin Bansal ,&nbsp;Sumeet Gupta ,&nbsp;Seema Bansal","doi":"10.1016/j.jtherbio.2026.104375","DOIUrl":"10.1016/j.jtherbio.2026.104375","url":null,"abstract":"<div><div>Climatological variations, triggered by global warming and rising temperatures, have become a growing concern, posing challenges to communities across the United States, Europe, and Asia. Earth heats, climate patterns shift, and the resulting climate instability triggers more persistent and powerful heatwaves, leading to significant ecological and health-related consequences. Disturbances in thermoregulation can lead to elevated core body temperature (CBT&gt;39 °C); this typically occurs during heat stress (HS), a state wherein the body's capacity to cool itself is challenged by multiple external (environmental conditions, pathogens) or internal factors (inflammatory, metabolic, hormonal, and neurological), often precipitating systemic inflammation and multiple organ failure. HS pathological cascade involves different interconnected processes like oxidative stress, inflammation, compromised circulation, disrupted blood-brain barrier (BBB), coagulation irregularities, organ-specific responses, electrolyte imbalances, heat shock proteins (HSPs), and interactions with pre-existing conditions. To effectively address this emerging public health issue, a combined approach is needed, like incorporating pharmacological treatments such as non-steroidal anti-inflammatory drugs (NSAIDs), diuretics, muscle relaxants, vasodilators, beta-blockers, and anti-anxiety agents with essential non-pharmacological supports like public health education, cooling centres, early detection systems, and individualized plans specifically designed for high-risk groups. This review provides insight into the concept of heat-induced injury on the cellular level, the worldwide prevalence of HS, the pathogenic mechanisms behind Heat Stress-induced Multiple Organ Dysfunction (HS-MOD), and the various therapeutic strategies available.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"136 ","pages":"Article 104375"},"PeriodicalIF":2.9,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146202072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heat shock response-driven signature and DNAJB11 in HCC: A multifaceted role in prognosis, immune microenvironment, ferroptosis and therapeutic sensitivity","authors":"Wenhan Geng ,&nbsp;Tiantian Li ,&nbsp;Jinglin Zhang ,&nbsp;Muzheng Shen ,&nbsp;Jinrui Zhang ,&nbsp;Jinghan Li ,&nbsp;Xiaojie Ma ,&nbsp;Jian Chen ,&nbsp;Haojin Sun ,&nbsp;Xiaopeng Liu ,&nbsp;Pengxiu Cao ,&nbsp;Yumei Fan ,&nbsp;Ke Tan","doi":"10.1016/j.jtherbio.2026.104415","DOIUrl":"10.1016/j.jtherbio.2026.104415","url":null,"abstract":"<div><div>The heat shock response (HSR) is a conserved cellular mechanism that safeguards cells against various stressors by inducing heat shock proteins (HSPs). However, to date, no study has employed the HSR to develop a prognostic risk model aimed at predicting the outcomes and directing the treatment strategies for hepatocellular carcinoma (HCC) patients. In this study, we identified two distinct molecular subgroups of HCC patients based on the expression of 37 key HSR-related genes (HRGs). These subgroups exhibited significant differences in immune infiltration, drug responsiveness, and immunotherapy efficacy. Notably, cluster 1 (C1) patients showed greater sensitivity to chemotherapy, while C2 patients were more responsive to immunotherapy. Six core HRGs (CD4, CDK5, CDKN2A, DNAJB11, HBB and TRPV4) were identified as potential biomarkers through machine learning algorithms. A risk score model incorporating these HRGs was developed to predict HCC prognosis. The expression of these HRGs was validated using immunohistochemistry (IHC), single-cell RNA sequencing, and spatial transcriptomics. Importantly, core HRGs were significantly correlated with ferroptosis, and RSL3 treatment markedly affected the expression levels of these HRGs in HCC cells. Moreover, knockdown of DNAJB11 significantly suppressed cell growth, inhibited migratory and invasive capacities, and enhanced RSL3-induced ferroptosis. This study provides the first comprehensive analysis of HSR in HCC, offering a novel molecular classification and prognostic tool to guide personalized treatment strategies.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"136 ","pages":"Article 104415"},"PeriodicalIF":2.9,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146227521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Thermal sensitivity of metabolic rate in a hemimetabolous insect: variation across life stages","authors":"Carmen Rolandi ,&nbsp;Silvina A. Belliard ,&nbsp;Pablo E. Schilman","doi":"10.1016/j.jtherbio.2026.104411","DOIUrl":"10.1016/j.jtherbio.2026.104411","url":null,"abstract":"<div><div>Environmental temperature strongly influences insect metabolic rate (MR), yet the thermal sensitivity of MR across developmental stages remains understudied in hemimetabolous species. Here we quantified resting metabolic rate (RMR) thermal sensitivity in <em>Rhodnius prolixus</em>, a hemimetabolous insect and an important Chagas disease vector. For that, we measured real-time CO₂ production by open flow-respirometry as a proxy for MR at six temperatures (10, 15, 20, 25, 30, and 35 °C) across all life stages. We found a stage-specific thermal sensitivity of the RMR. Instantaneous Q₁₀ estimates revealed ontogenetic variation: early nymphs exhibited higher thermal sensitivity at cooler temperatures and lower sensitivity at warmer temperatures, while adults maintained constant Q₁₀ values across the thermal range. These findings provide novel insights into thermal biology of hemimetabolous insects and show that developmental stage influences metabolic responses to temperature. Understanding these patterns is crucial for predicting how climate warming may affect the physiology, population dynamics and disease transmission potential of this vector species.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"136 ","pages":"Article 104411"},"PeriodicalIF":2.9,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146257527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Thermal and photic modulation of human sleep architecture and autonomic adaptation during an Antarctic summer expedition","authors":"Marek Sokol ,&nbsp;Petr Volf ,&nbsp;Jakub Holuša ,&nbsp;Michael Matějka ,&nbsp;Jan Hejda ,&nbsp;Patrik Kutílek","doi":"10.1016/j.jtherbio.2026.104403","DOIUrl":"10.1016/j.jtherbio.2026.104403","url":null,"abstract":"<div><div>Human sleep is strongly influenced by thermoregulatory and circadian processes, both of which are challenged in polar environments characterized by continuous daylight, low ambient temperatures, and high variability in solar radiation. This study examined how thermal and photic conditions modulate sleep architecture and autonomic function during the 2025 Czech Antarctic Expedition at James Ross Island. Ten expedition members were continuously monitored using validated wearable sensors that recorded sleep stages, heart rate, and respiratory rate across pre-expedition, Antarctic, and post-expedition phases. Environmental variables, including air temperature, relative humidity, and global radiation, were concurrently logged indoors and outdoors. Linear mixed-effects models revealed that the Antarctic phase was associated with a significant increase in slow-wave sleep (17.8 <span><math><mo>±</mo></math></span> 4.1% pre-expedition vs. 20.2 <span><math><mo>±</mo></math></span> 4.3% during expedition, <span><math><mrow><mi>p</mi><mo>&lt;</mo><mn>0</mn><mo>.</mo><mn>001</mn></mrow></math></span>) and a decrease in light sleep (<span><math><mrow><mi>p</mi><mo>=</mo><mn>0</mn><mo>.</mo><mn>002</mn></mrow></math></span>). Higher outdoor temperatures predicted a greater proportion of deep sleep (<span><math><mrow><mi>β</mi><mo>=</mo><mn>2</mn><mo>.</mo><mn>00</mn></mrow></math></span>, <span><math><mrow><mi>p</mi><mo>&lt;</mo><mn>0</mn><mo>.</mo><mn>001</mn></mrow></math></span>), and lower humidity was associated with increased deep sleep (<span><math><mrow><mi>β</mi><mo>=</mo><mo>−</mo><mn>1</mn><mo>.</mo><mn>04</mn></mrow></math></span>, <span><math><mrow><mi>p</mi><mo>=</mo><mn>0</mn><mo>.</mo><mn>046</mn></mrow></math></span>). The resting heart rate rose during the expedition and then declined significantly afterward, indicating autonomic recovery. These findings suggest that human sleep exhibits adaptive reorganization under combined thermal and photic stress, with enhanced slow-wave sleep supporting physiological restoration in cold, high-variability environments. The results provide novel evidence of thermoregulatory coupling between environmental conditions, sleep architecture, and autonomic balance <em>in situ</em>.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"136 ","pages":"Article 104403"},"PeriodicalIF":2.9,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146125446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Circadian modulation of core temperature and thermoregulatory strain during live-fire compartment exposure in firefighters","authors":"Benoit Mauvieux ,&nbsp;Adrian Markov ,&nbsp;Stéphane Besnard ,&nbsp;Yvan Touitou ,&nbsp;Ben J. Edwards","doi":"10.1016/j.jtherbio.2026.104383","DOIUrl":"10.1016/j.jtherbio.2026.104383","url":null,"abstract":"<div><div>Firefighters are repeatedly exposed to extreme radiant and convective heat during live-fire training, yet the potential influence of circadian timing on their thermoregulatory tolerance remains unexplored. This study tested whether time-of-day modulates physiological strain during standardized container fire exposures. Twenty-one professional male firefighters completed two identical 40-min live-fire sessions on the same day: late-morning (09:00 h, heat-gain phase) and late-evening (21:30 h, heat-loss phase). Core temperature (ingestible sensor), heart rate, skin temperature, under-PPE temperature and humidity, body mass, total body water, and ratings of perceived exertion were recorded. Environmental conditions were strictly matched between sessions. Core temperature rose faster and higher in the morning (ΔTcore +1.10 ± 0.25 °C; slope 0.028 °C·min⁻¹) than in the evening (+0.49 ± 0.21 °C; 0.012 °C·min⁻¹), despite similar peak values. Post-exposure cooling was slower in the morning (−0.37 vs −0.63 °C·h⁻¹), with a delayed hypothermic rebound. Morning sessions also elicited higher heart rates, greater perceived exertion, larger body-mass and water losses, and higher sub-garment humidity. These findings demonstrate that circadian phase significantly influences heat storage and recovery, with late-morning exposures imposing greater thermophysiological strain under identical workloads. Incorporating chronobiological principles into firefighter training schedules may reduce heat-related risk and optimize recovery strategies in extreme environments.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"136 ","pages":"Article 104383"},"PeriodicalIF":2.9,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146024132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated transcriptomic and metabolomic analyses reveal the molecular adaptive mechanisms of Schizopygopsis chengi baoxingensis under acute heat stress","authors":"Hangyu Lin ,&nbsp;Xiaoyun Wu ,&nbsp;Ya Liu ,&nbsp;Jun Ou ,&nbsp;Luyun Ni ,&nbsp;Feiyang Li ,&nbsp;Jiansheng Lai ,&nbsp;Quan Gong ,&nbsp;Jue Lin ,&nbsp;MingJiang Song","doi":"10.1016/j.jtherbio.2026.104398","DOIUrl":"10.1016/j.jtherbio.2026.104398","url":null,"abstract":"<div><div>Global warming alters aquatic habitats, challenging cold-water fish survival. <em>Schizopygopsis chengi baoxingensis</em>, a cold-water fish from the Qingyijiang River in China, is vulnerable to temperature changes. This study aimed to elucidate the molecular mechanisms underlying the response of <em>S. c. baoxingensis</em> to acute heat stress through an integrated approach of histopathological, transcriptomic, and metabolomic analyses. Histopathological results revealed significant tissue damage in both the liver and gills, with the liver showing pronounced hepatocellular vacuolization and nuclear displacement, and the gills exhibiting hyperplasia and partial lamellar necrosis. Transcriptomic analysis identified numerous differentially expressed genes (DEGs) in both tissues. In the liver, DEGs were enriched in pathways related to antioxidant stress, detoxification, and immune response. Notably, genes encoding glutathione S-transferase (GST) and UDP-glucuronosyltransferase (UGT) were upregulated, suggesting enhanced antioxidant and detoxification capabilities. Additionally, immune-related genes such as C2, C3, C4, C6, C9, CFB, CFH, and MASP2 were predominantly upregulated in the complement and coagulation cascades pathway. In the gills, DEGs including NPNT and NOS2 were significantly upregulated, suggesting enhanced respiratory function. Metabolomic profiling showed significant alterations in energy metabolism, with increased levels of NAD and NADH in the liver, indicating heightened energy demands under thermal stress. Our findings provide valuable insights into the molecular mechanisms underlying the heat stress response of <em>S. c. baoxingensis</em> and highlight potential targets for protecting this species under climate change.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"136 ","pages":"Article 104398"},"PeriodicalIF":2.9,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146024147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prewarming and thermal manipulation of long stored eggs: effect on embryonic development, hatching traits, body weight, rectal temperature, carcass, and organ traits at 3 days of age in broiler chickens","authors":"Nasir Abdallah ,&nbsp;Mikail Baylan ,&nbsp;Kadriye Kursun ,&nbsp;Gulsen Copur Akpinar","doi":"10.1016/j.jtherbio.2026.104407","DOIUrl":"10.1016/j.jtherbio.2026.104407","url":null,"abstract":"<div><div>This study investigated the effect of prewarming (PD) and thermal manipulation (TM) of long-stored eggs on embryonic development, hatching traits, body weight (BW), rectal temperature (RT), carcass, and organ traits in broilers at 3 days of age (DOA). Hatching eggs were exposed 2 PD treatments (6 h and 9-h) after storage. Each PD treatment was further divided into 2 groups and either incubated with a standard temperature (SI, 37.5 °C) or exposed to high temperature (TM, 39 °C between ED 5 and 17). Therefore, four experimental treatments in a completely randomized design (2 × 2 factorial experimental design) were created as follows: Eggs exposed to 6-h prewarming duration + standard incubation temperature (6-PDSI), eggs exposed to 9-h prewarming duration + standard incubation temperature (9-PDSI), eggs exposed to 6-h prewarming + high incubation temperature (6-PDTM) and eggs exposed to 9-h prewarming + high incubation temperature (9-PDTM). The incubation temperature (INC-T) had no significant effect on egg weight (EW) and egg weight loss (EW-L) (<em>P&gt;0.05</em>). The PD only influenced the Ew-L at embryonic day (ED) 10, which was higher in eggs preheated for 6 h (6-PD) than in eggs preheated for 9 h (9-PD) (<em>P&lt;0.05</em>). The weight of yolk-free body mass (YFBM-w), embryo length (Em-L), and beak length (BL) at ED 15 and the embryo + yolk weight (W-EY), YFBM-w, Em-L, tibia length (TL), and BL at ED 18 were higher in the 6-PD than in the 9-PD (<em>P&lt;0.05</em>). The Embryo + yolk yield (EY-Y), YFBM-w, yield of the yolk-free body mass (YFBM-Y), and Em-L at ED 15 and the W-EY, YFBM-w, and Em-L at ED 18 were significantly higher in TM than in SI embryos (<em>P&lt;0.05</em>). However, the eye width (Ey-w) at ED 15 was higher in the SI than in the TM embryos (<em>P&lt;0.05</em>). The INC-T and PD had no significant effect on hatchability, embryonic mortality, chick weight, and chick yield (<em>P&gt;0.05</em>)<strong>.</strong> However, the chick length was higher in the SI chicks (<em>P&lt;0.05</em>). The body weight, heart, liver, thigh, and intestinal traits were higher in 9-PD chicks, while rectal temperature was lowest in 6-PD chicks (<em>P&lt;0.05</em>). The thigh yield (TH-y) was higher in the TM than in the SI chicks (<em>P&lt;0.05</em>).</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"136 ","pages":"Article 104407"},"PeriodicalIF":2.9,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146170256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}