Journal of thermal biology最新文献

{"title":"Probiotic effects of Saccharomyces cerevisiae on immune response, testicular function, serum pituitary-gonadal hormones and stress markers following localized scrotal heat exposure","authors":"Edmund C. Mbegbu ,&nbsp;Ikechukwu R. Obidike ,&nbsp;Chinelo G. Ikele ,&nbsp;Patience C. Ugwu ,&nbsp;Chuka Ezema ,&nbsp;Lawrence O. Aka ,&nbsp;Abdussamad M. Abdussamad ,&nbsp;John J. Parrish","doi":"10.1016/j.jtherbio.2025.104261","DOIUrl":"10.1016/j.jtherbio.2025.104261","url":null,"abstract":"<div><div>Owing to the anti-inflammatory and anti-oxidant benefits of <em>Saccharomyces cerevisiae</em> (SC), 20 mature male albino rats, assigned into four groups (A-D; n = 5), were used to investigate its ameliorative effects on heat stress-induced testicular and humoral alterations. Group A rats were neither treated with SC nor exposed to heat [-SC, -HS]. Group B rats were treated with 7 mg/kg of SC<em>,</em> but were not exposed to heat [+SC, -HS]. Group C rats did not receive 7 mg/kg of SC<em>,</em> but were exposed to heat at 43 °C for 15 min [-SC, +HS]. Group D rats were treated with 7 mg/kg of SC<em>,</em> and were exposed to heat at 43 °C for 15 min [+SC, +HS]. Probiotic administration was done orally every other day for five consecutive weeks, when blood and testicular samples were collected for analyses. Probiotic treatment after heat exposure significantly increased (<em>p</em> &lt; 0.05) the total WBC, lymphocyte count, neutrophil count and monocyte count. Cortisol concentration significantly increased (<em>p &lt; 0.05</em>) in Groups B-D, while only Group C had a significantly higher (<em>p &lt; 0.05</em>) total cholesterol. Exposure to heat (in Groups C and D) significantly decreased (p &lt; 0.05) the mean left and right testicular weights, as well as the testicular and epididymal sperm counts. The histology of the testis revealed regeneration of the germinal epithelium of the seminiferous tubules in the group exposed to heat and treated with SC. Findings from this work proved that the probiotic potentials of SC could be harnessed for mitigating heat-induced humoral and spermatogenic alterations in male albino rats.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"132 ","pages":"Article 104261"},"PeriodicalIF":2.9,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145019010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of temperature-humidity index on heat stress behavior and welfare of battery-caged yellow-feathered native broilers during summertime in Hong Kong: An observational study","authors":"Theethawat Uea-Anuwong ,&nbsp;Yi Kei Lam ,&nbsp;Denis Yau ,&nbsp;Cheuk Ming Li ,&nbsp;Chen Xin ,&nbsp;Surya Paudel ,&nbsp;Anne Conan ,&nbsp;Dirk Udo Pfeiffer ,&nbsp;Ákos Kenéz","doi":"10.1016/j.jtherbio.2025.104224","DOIUrl":"10.1016/j.jtherbio.2025.104224","url":null,"abstract":"<div><div>This observational study investigated the effects of the temperature-humidity index (THI) on heat stress behavior, hormonal response, and body temperature of battery-caged yellow-feathered broilers on a Hong Kong poultry farm equipped with ventilation fans and foggers from August to September 2023. Twenty-six units distributed in the sidewall and inner zones of the chicken house were randomly selected for real-time THI measurement using data loggers. Each unit comprised six cages with three birds each. All chickens in the selected cages were scored for heat stress behaviors (n = 468). The body temperature and live weight of one randomly selected chicken per cage were measured on days 72 and 90 of age (n = 156). Two broilers per unit were randomly selected for blood corticosterone (n = 52). Although the sidewall zones were more exposed to sunlight than the inner zones, the data loggers showed no difference in the THI between these zones. Forty-five out of 468 chickens (9.6 %, 95 % CI 44.97–45.03) exhibited high heat stress behavior under high THI conditions. The THI and the zones showed no statistically significant associations with our observations of heat stress behavior and body weight change. The serum corticosterone levels of individual broilers measured in the afternoon were consistently lower than those measured in the morning; however, no significant difference was detected between the different zones of the house. In conclusion, the relatively small proportion of birds exhibiting heat stress behavior suggests that the native yellow-feathered broilers had considerable adaptive capacity to tolerate the hot and humid climate during summer in Hong Kong.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"132 ","pages":"Article 104224"},"PeriodicalIF":2.9,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144858183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The impact of climate change on the distribution of serpulid polychaetes: an ecophysiological approach","authors":"J. Pablo Sánchez-Ovando ,&nbsp;Fernando Díaz ,&nbsp;Luis Enrique Angeles-Gonzalez ,&nbsp;Fabiola Lafarga-De la Cruz ,&nbsp;Francisco Benítez-Villalobos ,&nbsp;Denise Re-Araujo","doi":"10.1016/j.jtherbio.2025.104228","DOIUrl":"10.1016/j.jtherbio.2025.104228","url":null,"abstract":"<div><div>Temperature influences the physiological performance of marine ectotherms—affecting survival, growth, and reproduction—and ultimately shaping their distribution. However, knowledge of how ocean warming will impact sessile ectotherms, such as serpulid polychaetes, remains limited. We hypothesize that rising ocean temperatures will affect serpulid thermal performance, potentially altering their distribution patterns. By combining laboratory-derived ecophysiological data with satellite sea-surface temperature (SST) data, we evaluated the effects of ocean warming on the distribution of two serpulid species from distinct regions: the temperate northern Pacific (<em>Spirobranchus spinosus</em>) and the tropical eastern Pacific (<em>Spirobranchus</em> cf. <em>corniculatus</em>). Adult worms were collected from the wild and acclimated for 30 days at different temperature ranges (11–20 °C for <em>S. spinosus</em>; 24–33 °C for <em>S.</em> cf. <em>corniculatus</em>) to assess thermal tolerance limits (CTmax, CTmin), thermal tolerance window, optimal temperature, and thermal safety margins. These physiological data were integrated with SST data to model potential distributions for both present (2010–2020) and future (2090–2100, SSP5-8.5) climate change scenario. Thermal tolerance limits increased with acclimation temperatures in both species. However, <em>S.</em> cf. <em>corniculatus</em> exhibited similar CTmax (35.1 °C) values at 30 and 33 °C, suggesting it is approaching its upper thermal limits. Both species exhibited narrow thermal windows (<em>S. spinosus</em>: 37 °C²; <em>S.</em> cf. <em>corniculatus</em>: 71 °C²), characteristic of stenothermic species. The optimal temperatures were 14 °C for <em>S. spinosus</em> and 30 °C for <em>S.</em> cf. <em>corniculatus</em>. By century's end, thermal safety margins are predicted to decrease, leading to range retractions in <em>S. spinosus</em> and potential poleward expansions in <em>S.</em> cf. <em>corniculatus</em>. These findings underscore the importance of integrating physiological and environmental data to assess the vulnerability of sessile ectotherms to climate change. Although other factors such as pH, currents, and oxygen may influence future distributions, this study provides an important first step toward understanding climate change impacts on sessile marine ectotherms.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"132 ","pages":"Article 104228"},"PeriodicalIF":2.9,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144864010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel insights into heat tolerance via metabolomic analysis in Tibetan sheep","authors":"Qiang-Jun Wang ,&nbsp;Zhu Meng ,&nbsp;Jia-Hong Chen,&nbsp;Chun-Huan Ren,&nbsp;Zi-Jun Zhang","doi":"10.1016/j.jtherbio.2025.104230","DOIUrl":"10.1016/j.jtherbio.2025.104230","url":null,"abstract":"<div><div>Tibetan sheep are highly adaptable to cold and hypoxic environments, allowing them to thrive on the Qinghai‒Tibet Plateau. Although many studies have investigated the mechanisms underlying their cold tolerance and hypoxia adaptation, it is still unclear how Tibetan sheep cope with the challenges of heat stress (HS) when raised in southern China. In this study, Tibetan sheep and heat-tolerant breeds (Hu sheep and Huanghuai sheep) were selected, and we conducted physiological observations, biochemical measurements, and metabolomic analysis on serum from a nonheat stress (NH) group, where the temperature–humidity index (THI) was 59.13, and a heat-stressed group (THI = 82.78). The results revealed that HS significantly increased the rectal temperature, respiratory rate, and heart rate of Tibetan sheep, and these values were significantly greater than those in the heat-tolerant breeds. Serum antioxidant indices revealed that catalase activity, total antioxidant capacity, and total superoxide dismutase activity were significantly lower in Tibetan sheep than in Hu sheep under HS. Metabolomic analysis revealed that HS reduced the levels of serum antioxidant metabolites, such as creatine, ornithine, l-arginine, and guanosine, and increased the levels of the oxidative stress marker metabolite 3-nitrotyrosine in Tibetan sheep. Additionally, the ratio of serum antioxidant metabolites to 3-nitrotyrosine in Tibetan sheep was significantly lower than that in heat-tolerant breeds, and these ratios were correlated with physiological parameters. Therefore, these results suggest that Tibetan sheep are relatively sensitive to HS.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"132 ","pages":"Article 104230"},"PeriodicalIF":2.9,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144890265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exposure of an intertidal fish to simulated heatwaves and suspended sediment – a multistressor approach","authors":"Anna Carolina Resende ,&nbsp;Catarina Vinagre ,&nbsp;Alice Rogers","doi":"10.1016/j.jtherbio.2025.104260","DOIUrl":"10.1016/j.jtherbio.2025.104260","url":null,"abstract":"<div><div>Extreme climatic events, such as marine heatwaves (MHW) and increased suspended sediment concentration (SSC), are increasing in frequency and intensity, resulting in sudden changes to coastal environments, especially intertidal zones. Intertidal animals experience conditions that substantially fluctuate over temporal and spatial scales and therefore require the ability to physiologically tolerate these fluctuations. Since multiple stressors often co-occur and natural populations tend to respond to local environmental fluctuations, we aimed to investigate individual and combined effects of MHW and increased suspended sedimentation in <em>Forsterygion lapillum</em> from two neighbouring coastal areas with distinct water temperatures and wave current regimes by assessing fish oxygen consumption rate, mortality and weight loss. Results showed that in both <em>F. lapillum</em> populations, oxygen consumption rate and survival probability were unaffected by any treatment. However, fish from both populations lost weight during heatwave and multistressor treatments (i.e. heatwave and sedimentation), while fish from the sedimentation treatment alone did not lose weight. Although a direct effect on fish respiration was not found, our results indicate that <em>F. lapillum</em> performance is reduced when exposed to heatwaves individually and in combination with increased sediment suspension. Weight loss indicates that fish experiencing these stressors were unable to meet their metabolic demands.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"132 ","pages":"Article 104260"},"PeriodicalIF":2.9,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145003784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diel effects on the expression of heat shock protein genes (hsp70 and hsp90) in early developmental stages of the Black Southern cod Patagonotothen tessellata (Richardson, 1845).","authors":"Daniel Osvaldo Bruno, Cristina Fernanda Nardi, Canela Chaparro-Godoy, Mariela Fernanda Victorio","doi":"10.1016/j.jtherbio.2025.104220","DOIUrl":"10.1016/j.jtherbio.2025.104220","url":null,"abstract":"<p><p>As larvae, fish are more sensitive to stress factors. Therefore, understanding the cellular defence mechanisms during the early stage of life can help infer variations in the type of response depending on their development and the influence of rhythmic cycles in their activity. We aimed to analyse the influence of diel variation on the expression of stress response-related genes (hsp70 and hsp90) in Patagonothen tessellata's embryos and yolk sac larvae under an experimental heat shock treatment. Significantly higher expression levels of the hsp70 and hsp90 genes in P. tessellata yolk sac larvae were observed when the heat shock treatment was performed at nighttime than at daytime, indicating the species' diurnal behaviour. In addition, higher expression levels of the hsp70 and hsp90 genes were observed in yolk sac larvae compared to embryos, highlighting ontogenetic differences in the species' response to thermal stress. This variation may be attributed to differences in the allocation of endogenous energy reserves during early development. Furthermore, increased gene expression levels were detected 1 h after the heat shock, suggesting a rapid activation of this gene system early in life, once P. tessellata hatched. In order to elucidate the capability of early life stages of fish to cope with future scenarios related to climate change, daily rhythms must also be considered to ensure accurate physiological responses.</p>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"132 ","pages":"104220"},"PeriodicalIF":2.9,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144812200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Population-specific patterns of longevity and thermal induced lifespan extension in Drosophila suzukii","authors":"A.C. Scannapieco ,&nbsp;D.F. Segura ,&nbsp;S.B. Lanzavecchia ,&nbsp;M.C. Liendo ,&nbsp;F.M. Norry ,&nbsp;P. Sambucetti","doi":"10.1016/j.jtherbio.2025.104229","DOIUrl":"10.1016/j.jtherbio.2025.104229","url":null,"abstract":"<div><div><em>Drosophila suzukii</em> is an invasive pest that damages fruits of soft skin, provoking economic losses for commercial fruit producers. Understanding its biology is key for developing pest management strategies, with particular attention to life-history traits such as longevity and thermal tolerance. While previous studies mainly analyzed temperature effects on longevity, response to sub-lethal thermal treatments and its potential beneficial effects on longevity have not been explored. We examined thermal induced changes in longevity and explored heat and cold tolerance in two <em>D. suzukii</em> stocks derived from climatically different regions of Argentina. Longevity was assessed at two constant temperatures (25 and 30 °C) and at 25 °C with either a heat or a cold-stress treatment. Differences in mean longevity between populations were dependent on temperature regimen and thermal-stress treatment, with differences being significant at 25 °C both with and without a heat-stress. A reduced longevity was observed for flies of both stocks exposed at constant 30 °C. Longevity extension induced by heat was found in flies from the temperate/subtropical region which were also the shortest-lived flies at 25 °C. No effects of the cold-stress treatment on longevity were observed. Regarding thermal tolerance, flies from the cold region were more heat-susceptible and recovered faster after cold-stress than flies from the temperate/subtropical region. Our results evidence heat-induced lifespan extension in <em>D. suzukii</em> and suggest that this phenomenon could be an important aspect of population phenotypic plasticity for this species.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"132 ","pages":"Article 104229"},"PeriodicalIF":2.9,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144880219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integration of multi-omics resources reveals genetic features associated with environmental adaptation in the Wuzhishan pig genome","authors":"Ziqi Zhong ,&nbsp;Feng Wang ,&nbsp;Xinfeng Xie ,&nbsp;Ziyi Wang ,&nbsp;Deyou Pan ,&nbsp;Zhen Wang ,&nbsp;Qian Xiao","doi":"10.1016/j.jtherbio.2025.104264","DOIUrl":"10.1016/j.jtherbio.2025.104264","url":null,"abstract":"<div><div>In light of the challenges posed by global climate change, the environmental adaptability of organisms is becoming increasingly important. The Wuzhishan (WZS) pig, tolerant to high heat and humidity, is an ideal model for genomic study. By characterizing its genome and assessing its genetic diversity and runs of homozygosity (ROH), we can gain insights into its current conservation status and genomic architecture. To this end, we analyzed whole-genome resequencing data from WZS pigs, identifying a comprehensive set of genetic variations: 12,844,858 single nucleotide polymorphisms (SNPs), 4,723,303 insertions and deletions (InDels), 39,311 copy number variations (CNVs), and 70,392 structural variations (SVs). ROH analysis revealed mostly short segments, indicating limited recent admixture and relatively low inbreeding, reflecting high genetic diversity. Linkage disequilibrium decay and effective population size analyses supported these findings, consistent with previous studies. Population genetic analyses of pig populations from Asia, Europe, and the Americas were conducted using SNPs, InDels, and SVs. The results obtained from different variant types were largely concordant, suggesting that the constructed genomic variation database is robust. Notably, the WZS pig displayed relatively unique genetic characteristics compared to other breeds. By intersecting the top 5 % of genes under selection signatures, we identified key pathways and candidate genes associated with important traits in WZS pigs, including reproduction (<em>ABCC9, APC2, CABS1, CSNK1G1</em>), immunity (<em>ARPIN, CMAS, ITPR1, SLC25A13</em>), meat quality (<em>GANC, OBSCN, SLC5A4</em>), environmental adaptability (<em>MAGI1, PHF3</em>), and heat tolerance (<em>GABPB1, LRRC25</em>). Further integrative analyses based on transcriptomic, single-cell transcriptomic, and epigenomic data were conducted to explore the regulatory mechanisms of key genes such as <em>LRRC25</em> and <em>ITPR1</em>. We identified relevant expression quantitative trait loci (eQTLs), associated tissues, and distal regulatory elements that potentially influence gene expression. These findings suggest that epigenetic modifications may contribute to the enhanced heat tolerance and immune function observed in WZS pigs. In conclusion, WZS pigs represent a valuable genetic resource. Further research on this breed will deepen our understanding of mechanisms underlying environmental adaptability, and provide important insights into evolutionary biology and breeding strategies for improved resilience in livestock.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"132 ","pages":"Article 104264"},"PeriodicalIF":2.9,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145010343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ventilatory and perceptual habituation achieved with twice-daily, six total brief 12 °C head-out water immersions","authors":"Afton D. Seeley ,&nbsp;Thomas A. Mayer ,&nbsp;Shaun C. Brazelton ,&nbsp;David H. Gonzalez Rojas ,&nbsp;Kathryn G. McCarthy ,&nbsp;Phillip O. Bodurtha ,&nbsp;Molly E. Heikkinen ,&nbsp;Gabrielle E.W. Giersch ,&nbsp;MariaLena A. Shaw ,&nbsp;Andrew M. Greenfield ,&nbsp;Karleigh E. Bradbury ,&nbsp;Koby C. Conz ,&nbsp;Benjamin J. Ryan ,&nbsp;Aaron R. Caldwell ,&nbsp;K. Riley Connor ,&nbsp;Billie K. Alba ,&nbsp;John W. Castellani","doi":"10.1016/j.jtherbio.2025.104216","DOIUrl":"10.1016/j.jtherbio.2025.104216","url":null,"abstract":"<div><div>Repetitive cold exposure can evoke habituation capable of reducing physiological stress, energy expenditure and improving comfort in the cold. This study sought to evaluate physiological responses to repeated 10-min 12 °C head-out cold immersions to determine whether metabolic habituation with lower temporal burden could be achieved. Fourteen males (25 ± 7 years, 23.8 ± 5.8% body fat) completed two water immersion sequences consisting of twice daily, six total 10-min head-out immersions, one in cold water (CWI, 12.0 ± 0.1 °C) and the other in thermoneutral water (TN, 35.0 ± 0.2 °C). Water immersion was completed twice per day, separated by at least 4 h, for three consecutive days wearing only compression shorts. CWI was unable to induce metabolic habituation evidenced by unchanged oxygen consumption (<em>p</em> = 0.281) across immersions. Six 12 °C CWIs were sufficient to reduce average 10-min V_E after just three immersions (IMM4: 22.12 ± 4.68 vs. IMM1: 30.90 ± 14.15 L min⁻¹, <em>p</em> &lt; 0.001), via reduced tidal volume (−0.252 [-0.446 to −0.058] L). Perceptual habituation was achieved, with cold exposure inducing shivering sensation habituation after 2 immersions (<em>p</em> = 0.002), thermal comfort improvement after 4 (<em>p</em> = 0.017), and thermal sensation elevation (<em>p</em> = 0.029) after the completion of 5 head-out immersions. Heart rate, mean arterial blood pressure, change in core temperature, and mean weighted skin temperature did not demonstrate evidence of cold-water habituation. In summary, a total of six 12 °C water head-out immersions provided an adaptive stimulus capable of inducing both ventilatory and perceptual habituation but unable to habituate cardiovascular, metabolic, or thermoregulatory cold response.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"132 ","pages":"Article 104216"},"PeriodicalIF":2.9,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144828702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of transcriptomic and proteomic changes in bovine myocytes subject to temporal heat stress","authors":"Erika Eckhardt ,&nbsp;Andrea Luttman ,&nbsp;Jayasimha R. Daddam ,&nbsp;Boon Hong Keng ,&nbsp;Wonseob Kim ,&nbsp;Cedric Gondro ,&nbsp;Jongkyoo Kim","doi":"10.1016/j.jtherbio.2025.104246","DOIUrl":"10.1016/j.jtherbio.2025.104246","url":null,"abstract":"<div><div>This study elucidated the degree of molecular response to heat stress (HS) in bovine myocytes by measuring changes in the transcriptome and proteome. Bovine satellite cells (BSCs) were extracted from holstein bull calves (n = 3, BW: 77.10 ± 2.02 kg). Following myogenic differentiation, myocytes were exposed to one of three treatment groups for 3 h: 38 °C (control; <strong>CON</strong>; n = 3), 39.5 °C (moderate heat stress; <strong>MHS</strong>; n = 3), and 41 °C (extreme heat stress; <strong>EHS</strong>; n = 3). RNA and protein were extracted for transcriptomic and proteomic sequencing analyses, respectively. Differentially expressed genes (DEGs) for the contrasts MHS vs. CON and EHS vs. CON were selected using an FDR adjusted significance threshold of <em>P</em> &lt; 0.05, regardless of Log₂ fold change, with differentially abundant proteins (DAPs) selected at an FDR adjusted significance threshold of <em>P</em> &lt; 0.05 and absolute fold change ± 1.2. DEGs were then subjected to gene set enrichment analysis. A total of 888 DEGs were detected for MHS and 2590 for EHS, with 590 DEGs shared between MHS and EHS. EHS resulted in elevated expression of multiple heat shock protein (HSP) isoforms especially those related to <em>HSP70</em>, in addition to increased expression of <em>FOXO6</em> and <em>PPARGC1A</em>. Alteration of key anabolic pathways associated with protein synthesis, modulation of myogenic regulatory factors, and the large number of DEGs detected in HS-myocytes demonstrates the speculation of phenotypic alterations to myotube size and protein synthesis.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"132 ","pages":"Article 104246"},"PeriodicalIF":2.9,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144913541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}