Doaa Ibrahim , Mohamed Shawky , Asmaa EL- Sayed Kamel , Ahmed Abdelfattah-Hassan , Rahma Taha , Samah S. Khalil , Mohamed Tharwat Elabbasy , Asmaa T.Y. Kishawy
{"title":"饲粮微藻混合物在缓解肉鸡热应激缩回中的重要作用:对肉鸡生产性能、肉类抗氧化剂和脂肪酸含量以及热休克蛋白和sirtuins相关基因表达的影响","authors":"Doaa Ibrahim , Mohamed Shawky , Asmaa EL- Sayed Kamel , Ahmed Abdelfattah-Hassan , Rahma Taha , Samah S. Khalil , Mohamed Tharwat Elabbasy , Asmaa T.Y. Kishawy","doi":"10.1016/j.jtherbio.2025.104222","DOIUrl":null,"url":null,"abstract":"<div><div>Adopting innovative nutritional strategies, such as microalgae blend supplementation, can help reduce the detrimental effects of heat stress (HS) in poultry farming. This study was designed to explore the effects of different dietary levels of microalgae blend containing <em>nannochloropsis oculate</em>, <em>schizochytrium</em> and <em>amphora coffesformis</em> on modulating growth performance, muscle's antioxidant status, lipid profile and the expression of heat shock proteins and sirtuins family related genes in heat-stressed chicks. A total of 250 one-day-old chicks were randomly assigned to five experimental groups, with five replicates of 10 birds each: a control group reared under normal environmental conditions and fed a basal diet; HS group exposed to heat stress and fed a basal diet; and three HS groups supplemented with a microalgae blend at 2.5, 5, and 10 g/kg of diet, respectively. A notable improvement in total growth and FCR especially was recorded in group exposed to HS and supplemented with 10 g/kg diet of microalgae blend compared to HS group. Moreover, the group exposed to HS and fortified with microalgae blend at the concentration of 10 g/kg diet showed reduced cholesterol and triacylglycerol levels, comparable to those of the control group. Notably, all heat-stressed groups supplemented with the microalgae blend showed no significant changes in blood parameters compared to the control group. In the heat-stressed group, dietary supplementation with 10 g/kg of the microalgae blend significantly reduced the levels of ALT, uric acid, AST, creatinine, corticosterone, and thyroid hormones (T3 and T4), bringing them closer to normal physiological ranges. With increasing levels of the microalgae blend, there was a marked enhancement in the activity of muscle antioxidant enzymes, including glutathione peroxidase (GSH-Px), catalase, and superoxide dismutase (SOD), along with an increase in T-AOC content, accompanied by a significant reduction in MDA levels. Notably, increasing the concentration of the microalgae blend resulted in elevated levels of n-3 PUFAs, particularly eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), in the breast muscle. Relative mRNA expression levels of <em>Hsp70, Hsp90, SIRT1, SIRT3</em>, and <em>SIRT7</em> were elevated in the heat-stressed groups; however, the group supplemented with 10 g/kg of the microalgae blend exhibited the most pronounced downregulation of these genes. In conclusion, this study underscores the potential of the microalgae blend to mitigate the adverse effects of heat stress in poultry farming via modulating antioxidant activity, upregulating expression of sirtuins family and attenuating the overexpression of heat shock proteins.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"132 ","pages":"Article 104222"},"PeriodicalIF":2.9000,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Promising role of dietary microalgae blend in alleviating the heat stress draw backs in broiler chickens: Impact on performance, meat antioxidants and fatty acids content and expression of heat shock protein and sirtuins related genes\",\"authors\":\"Doaa Ibrahim , Mohamed Shawky , Asmaa EL- Sayed Kamel , Ahmed Abdelfattah-Hassan , Rahma Taha , Samah S. Khalil , Mohamed Tharwat Elabbasy , Asmaa T.Y. Kishawy\",\"doi\":\"10.1016/j.jtherbio.2025.104222\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Adopting innovative nutritional strategies, such as microalgae blend supplementation, can help reduce the detrimental effects of heat stress (HS) in poultry farming. This study was designed to explore the effects of different dietary levels of microalgae blend containing <em>nannochloropsis oculate</em>, <em>schizochytrium</em> and <em>amphora coffesformis</em> on modulating growth performance, muscle's antioxidant status, lipid profile and the expression of heat shock proteins and sirtuins family related genes in heat-stressed chicks. A total of 250 one-day-old chicks were randomly assigned to five experimental groups, with five replicates of 10 birds each: a control group reared under normal environmental conditions and fed a basal diet; HS group exposed to heat stress and fed a basal diet; and three HS groups supplemented with a microalgae blend at 2.5, 5, and 10 g/kg of diet, respectively. A notable improvement in total growth and FCR especially was recorded in group exposed to HS and supplemented with 10 g/kg diet of microalgae blend compared to HS group. Moreover, the group exposed to HS and fortified with microalgae blend at the concentration of 10 g/kg diet showed reduced cholesterol and triacylglycerol levels, comparable to those of the control group. Notably, all heat-stressed groups supplemented with the microalgae blend showed no significant changes in blood parameters compared to the control group. In the heat-stressed group, dietary supplementation with 10 g/kg of the microalgae blend significantly reduced the levels of ALT, uric acid, AST, creatinine, corticosterone, and thyroid hormones (T3 and T4), bringing them closer to normal physiological ranges. With increasing levels of the microalgae blend, there was a marked enhancement in the activity of muscle antioxidant enzymes, including glutathione peroxidase (GSH-Px), catalase, and superoxide dismutase (SOD), along with an increase in T-AOC content, accompanied by a significant reduction in MDA levels. Notably, increasing the concentration of the microalgae blend resulted in elevated levels of n-3 PUFAs, particularly eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), in the breast muscle. Relative mRNA expression levels of <em>Hsp70, Hsp90, SIRT1, SIRT3</em>, and <em>SIRT7</em> were elevated in the heat-stressed groups; however, the group supplemented with 10 g/kg of the microalgae blend exhibited the most pronounced downregulation of these genes. In conclusion, this study underscores the potential of the microalgae blend to mitigate the adverse effects of heat stress in poultry farming via modulating antioxidant activity, upregulating expression of sirtuins family and attenuating the overexpression of heat shock proteins.</div></div>\",\"PeriodicalId\":17428,\"journal\":{\"name\":\"Journal of thermal biology\",\"volume\":\"132 \",\"pages\":\"Article 104222\"},\"PeriodicalIF\":2.9000,\"publicationDate\":\"2025-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of thermal biology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0306456525001792\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of thermal biology","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0306456525001792","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOLOGY","Score":null,"Total":0}
Promising role of dietary microalgae blend in alleviating the heat stress draw backs in broiler chickens: Impact on performance, meat antioxidants and fatty acids content and expression of heat shock protein and sirtuins related genes
Adopting innovative nutritional strategies, such as microalgae blend supplementation, can help reduce the detrimental effects of heat stress (HS) in poultry farming. This study was designed to explore the effects of different dietary levels of microalgae blend containing nannochloropsis oculate, schizochytrium and amphora coffesformis on modulating growth performance, muscle's antioxidant status, lipid profile and the expression of heat shock proteins and sirtuins family related genes in heat-stressed chicks. A total of 250 one-day-old chicks were randomly assigned to five experimental groups, with five replicates of 10 birds each: a control group reared under normal environmental conditions and fed a basal diet; HS group exposed to heat stress and fed a basal diet; and three HS groups supplemented with a microalgae blend at 2.5, 5, and 10 g/kg of diet, respectively. A notable improvement in total growth and FCR especially was recorded in group exposed to HS and supplemented with 10 g/kg diet of microalgae blend compared to HS group. Moreover, the group exposed to HS and fortified with microalgae blend at the concentration of 10 g/kg diet showed reduced cholesterol and triacylglycerol levels, comparable to those of the control group. Notably, all heat-stressed groups supplemented with the microalgae blend showed no significant changes in blood parameters compared to the control group. In the heat-stressed group, dietary supplementation with 10 g/kg of the microalgae blend significantly reduced the levels of ALT, uric acid, AST, creatinine, corticosterone, and thyroid hormones (T3 and T4), bringing them closer to normal physiological ranges. With increasing levels of the microalgae blend, there was a marked enhancement in the activity of muscle antioxidant enzymes, including glutathione peroxidase (GSH-Px), catalase, and superoxide dismutase (SOD), along with an increase in T-AOC content, accompanied by a significant reduction in MDA levels. Notably, increasing the concentration of the microalgae blend resulted in elevated levels of n-3 PUFAs, particularly eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), in the breast muscle. Relative mRNA expression levels of Hsp70, Hsp90, SIRT1, SIRT3, and SIRT7 were elevated in the heat-stressed groups; however, the group supplemented with 10 g/kg of the microalgae blend exhibited the most pronounced downregulation of these genes. In conclusion, this study underscores the potential of the microalgae blend to mitigate the adverse effects of heat stress in poultry farming via modulating antioxidant activity, upregulating expression of sirtuins family and attenuating the overexpression of heat shock proteins.
期刊介绍:
The Journal of Thermal Biology publishes articles that advance our knowledge on the ways and mechanisms through which temperature affects man and animals. This includes studies of their responses to these effects and on the ecological consequences. Directly relevant to this theme are:
• The mechanisms of thermal limitation, heat and cold injury, and the resistance of organisms to extremes of temperature
• The mechanisms involved in acclimation, acclimatization and evolutionary adaptation to temperature
• Mechanisms underlying the patterns of hibernation, torpor, dormancy, aestivation and diapause
• Effects of temperature on reproduction and development, growth, ageing and life-span
• Studies on modelling heat transfer between organisms and their environment
• The contributions of temperature to effects of climate change on animal species and man
• Studies of conservation biology and physiology related to temperature
• Behavioural and physiological regulation of body temperature including its pathophysiology and fever
• Medical applications of hypo- and hyperthermia
Article types:
• Original articles
• Review articles