Journal of thermal biology最新文献

{"title":"Transcriptomic profiling revealed the regulatory pathways and key genes associated with cold tolerance in two eel gobies","authors":"Jing Liu ,&nbsp;Yantao Liu ,&nbsp;Tianwei Liu ,&nbsp;Cheng Zhao ,&nbsp;Yuzhen Wang ,&nbsp;Kun Huang ,&nbsp;An Xu ,&nbsp;Liqin Liu ,&nbsp;Li Gong ,&nbsp;Zhenming Lü","doi":"10.1016/j.jtherbio.2025.104136","DOIUrl":"10.1016/j.jtherbio.2025.104136","url":null,"abstract":"<div><div>Closely related species of the eel goby family (Gobiidae) have evolved divergent resistance to low temperatures, but the molecular mechanisms remain poorly understood. This study used a comparative transcriptomic approach to identify key pathways and genes associated with cold tolerance in two eel goby species. Expression profiles of the cold-tolerant <em>O. lacepedii</em> and the cold-sensitive <em>O. rebecca</em> in control (23 °C) and cold stress groups (15 °C and 11 °C) were analyzed. Differentially expressed genes closely linked to interspecific cold tolerance divergence were identified through transcriptome profiling and Venn diagram analysis. GO and KEGG enrichment analyses revealed that processes related to cellular homeostasis, the PPAR signaling pathway, cellular respiration, and oxidative phosphorylation were activated during the cold tolerance response of eel gobies. WGCNA analysis indicated that the hub genes related to thermogenesis and microtubular stability, specifically <em>PPARGC1A</em> and <em>α-tubulin,</em> may contribute to the high cold tolerance in <em>O. lacepedii.</em> These findings provide key clues for dissection of the molecular mechanisms behind the formation of cold tolerance in eel gobies.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"130 ","pages":"Article 104136"},"PeriodicalIF":2.9,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144105896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research on recognition of bedding system coverage rate using infrared thermal imaging","authors":"Haonan Ma ,&nbsp;Guodan Liu ,&nbsp;Yulei Lin ,&nbsp;Guodong Zhu ,&nbsp;Peiqi Zhang ,&nbsp;Mingli Lu ,&nbsp;Songtao Hu","doi":"10.1016/j.jtherbio.2025.104146","DOIUrl":"10.1016/j.jtherbio.2025.104146","url":null,"abstract":"<div><div>This study investigated a new challenging problem: how to recognize bedding system coverage rate (BSCR) in real-time without contact. To overcome this problem, a method combining infrared thermal imaging with image segmentation algorithms was proposed. The infrared thermal images of 30 subjects with common coverage forms were captured under three shooting angles (45°, 60°, 90°) and three ambient temperatures (23 °C, 26 °C, 29 °C) to evaluate the effect of these factors on recognition accuracy. The proposed method was then applied in real-time to recognize BSCR in summer. And the subjective thermal perceptions (thermal sensation votes, thermal comfort votes) and objective sleep quality were collected. Three image segmentation methods were tested. And the K-means clustering excelled in recognizing BSCR, achieving an MAE of 3.70, RMSE of 4.67, and <span><math><mrow><msup><mi>R</mi><mn>2</mn></msup></mrow></math></span> of 0.901. The optimal shooting angle for infrared camera was 60°. Additionally, the summer indoor temperature (23 °C–29 °C) had no significant impact on the recognition accuracy. The average BSCR during sleep was 76.6 % under conditions of thermal comfort and good sleep quality. Notably, females exhibited the BSCR that was 8.9 % higher than that of males (p &lt; 0.01). This study provides reference data and insights for establishing a non-contact sleep thermal comfort prediction model.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"130 ","pages":"Article 104146"},"PeriodicalIF":2.9,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144134507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Head-neck heating and fatigue: Effects on peripheral and central function and motor accuracy in healthy men and those with multiple sclerosis","authors":"Gintare Dauksaite ,&nbsp;Nerijus Eimantas ,&nbsp;Rima Solianik ,&nbsp;Laura Daniuseviciute-Brazaite ,&nbsp;Lina Malciene ,&nbsp;Marius Brazaitis","doi":"10.1016/j.jtherbio.2025.104131","DOIUrl":"10.1016/j.jtherbio.2025.104131","url":null,"abstract":"<div><h3>Objective</h3><div>This study aimed to evaluate whether localized heating of the head and neck, combined with prolonged submaximal exercise at a thermoneutral temperature, would elicit peripheral and central changes in healthy men and those with multiple sclerosis (MS).</div></div><div><h3>Methods</h3><div>We studied the effects of head<strong>-</strong>neck heating (at 44 °C ± 0.5 °C adjacent to the skin) in men with relapsing-remitting MS (n = 16; age: 31.8 ± 7.6 years) and healthy men (n = 18; age: 27.6 ± 4.3 years). Both groups completed local heating and control trials (1-week apart) comprising 100 intermittent isometric knee extensions (5-s contraction, 20-s rest). We assessed electrically induced torque, maximal voluntary contraction (MVC), central activation ratio, muscle temperature (Tmu), perceived effort, blood stress markers, electromyography (EMG), and motor variability and accuracy. Measurements were taken at baseline, after 50 and 100 repetitions, and after 1-h recovery.</div></div><div><h3>Results</h3><div>Local heating inhibits central activation and reduces exercise-induced peripheral fatigue compared with non-heating conditions (<em>p</em> &lt; 0.05) in men with MS. Head-neck heating led to a greater increase in Tmu during exercise in both groups. During high-intensity exercise (75 % MVC), local heating increased perceived effort in men with MS, impaired accuracy in healthy men, but did not affect EMG amplitude in either group (<em>p</em> &lt; 0.05). Local heating during submaximal exercise induced greater changes in central and peripheral fatigability in men with MS than in healthy men (<em>p</em> &lt; 0.05).</div></div><div><h3>Conclusion</h3><div>Head-neck heating during exercise suppressed central muscle activation and reduced peripheral fatigue in men with MS more than exercise performed under non-heating conditions.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"130 ","pages":"Article 104131"},"PeriodicalIF":2.9,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143935228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bayesian neural networks for probabilistic modeling of thermal dynamics in multiscale tissue engineering scaffolds","authors":"Janjhyam Venkata Naga Ramesh ,&nbsp;Abhilash Sonker ,&nbsp;G. Indumathi ,&nbsp;D. Balakrishnan ,&nbsp;Divya Nimma ,&nbsp;Jayavarapu Karthik","doi":"10.1016/j.jtherbio.2025.104134","DOIUrl":"10.1016/j.jtherbio.2025.104134","url":null,"abstract":"<div><div>Multiscale tissue engineering integrated with thermal dynamics exhibits a critical role as scaffolds in maintaining the structural integrity and functionality in fabrication applications. Thermal dynamics influence the properties such as thermal conductivity, the capacity of heat, and thermal expansion of materials to maintain the scaffold stability in various physiological conditions. However, the scaffold's heat distribution in a uniform manner is varied due to variations in pore size and geometrics. Additionally, variation in scales affects the thermal gradient impacts on the cell growth and integrity.</div><div>This paper proposes the 3D Scaffolds Probabilistic Weighted Bayesian Neural Network (3D-SP-WBNN) for tissue engineering with a multiscale scaffold model. It uses the 3D scaffolds for the multiscale design estimation. The weighted Probabilistic Bayesian Neural Network model is employed for estimating features in tissues and evaluating the cell growth and proliferation.</div><div>Thermal gradients measured were in between 1 °C and 4 °C for human bone, skin, and cartilage tissues. For low and moderate temperatures of 1 °C and 2 °C, the cell proliferation rates in cartilage tissues were 15–20 % per day. The scaffold design uses the Hybrid Hydrogel/PCL composite to achieve a higher proliferation rate of 25–35 % per day. The estimated forecasting achieves an accuracy range of 82–96 % for different cell densities and thermal conditions.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"130 ","pages":"Article 104134"},"PeriodicalIF":2.9,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144071080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of exercise intensity on skin temperature and muscle oxygen saturation in the thigh during and after strength resistance training","authors":"Aldo A. Vasquez-Bonilla ,&nbsp;Federico Boschetti ,&nbsp;Rodrigo Yañez Sepulveda ,&nbsp;Manuel Sillero-Quintana","doi":"10.1016/j.jtherbio.2025.104132","DOIUrl":"10.1016/j.jtherbio.2025.104132","url":null,"abstract":"<div><div>This study aimed to describe and correlate the skin temperature (Tsk) and muscle oxygen saturation (SmO₂) during leg extension exercises with higher load (HL) and lower-load (LL) demands. Seventeen active men (age: 25.6 ± 3.4 years) participated by performed 3 x 15 repetitions of leg extension at 70 % of 1RM (HL) and 35 % of 1RM (LL). The Tsk of the thigh, knees, and legs were recorded with a thermal camera. SmO₂ was assessed using non-invasive near-infrared spectroscopy (NIRS) technology. The percentage of muscle oxygen extraction (∇%SmO₂) and the hyperaemic response (Δ%SmO2) in the vastus lateralis (VL) was calculated. Also, the rating of perceived exertion (RPE) scale data was obtained. Data were collected after warm-up (Baseline), after the first set (Set 1), second set (Set 2), third set (Set 3), and twice during recovery (7 min and 15 min). The results indicate an increase in Tsk thigh from baseline to recovery, which was more differenced in the HL group (Tsk = 30.44 ± 1.24 °C to 31.38 ± 1.32 °C) than in the LL group (Tsk = 30.86 ± 0.96 °C to 31.32 ± 1.06 °C). Similarly, SmO₂ decreased during exercise, more in the HL group than in the LL group (SmO₂ = 18 ± 15 % vs. 23 ± 15 %), and ∇%SmO₂ increased more in the HL group than in the LL group (SmO₂ = 75 ± 20 % vs. 65 ± 20 %). Additionally, during recovery, the hyperaemic response was higher in HL group than in LL group (SmO₂ = 12 ± 14 % vs. 3.1 ± 9.1 %). The RPE was also higher in HL group (9.5 ± 0.6) than in LL group (4.8 ± 1.3). Moreover, the increase in Tsk thigh was associated with ∇%SmO₂ during exercise (r² = 0.43 to 0.55) and recovery (r² = 0.31 to 0.52). Tsk and SmO₂ metrics reflect metabolic changes occurring during and after resistance training, which can be useful for monitoring the internal response to the workout.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"130 ","pages":"Article 104132"},"PeriodicalIF":2.9,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144116860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Using curcumin (Curcuma longa) as a dietary supplement modulates performance, behavior, blood metabolites, antioxidant status, and histomorphological changes in heat-stressed broiler chickens","authors":"Heba S.A. Gharib ,&nbsp;Asmaa I. Abdelaty ,&nbsp;Walaa M.S. Gomaa ,&nbsp;Ahmed Gouda ,&nbsp;Hala Y. Amer ,&nbsp;Sally A.M. Mohamed ,&nbsp;Rasha I.M. Hassan","doi":"10.1016/j.jtherbio.2025.104144","DOIUrl":"10.1016/j.jtherbio.2025.104144","url":null,"abstract":"<div><div>This study aimed to evaluate the effect of curcumin supplementation on growth performance, carcass parameters, behavior characteristics, blood metabolites, antioxidant status, and tissue histomorphology in heat-stressed broiler chickens. 120 one-day-old chicks were reared over 35 days in two separate rooms; one designated to induce heat stress and the other to maintain a normal temperature. After a completely randomized design, birds were divided into four treatments, each with 6 replicates of 5 chicks. Treatments were thermoneutral control group fed basal diet, heat-stressed group fed basal diet, curcumin-supplemented group fed basal diet with 1g/kg curcumin, and curcumin-supplemented heat-stressed group fed basal diet with 1g/kg curcumin. Broilers supplemented with curcumin and reared under heat stress conditions grew significantly (P &lt; 0.05) more than other groups with improved carcass parameters. The proportion of birds exhibiting standard behavior was enhanced (P &lt; 0.05), while the duration of Tonic immobility test was significantly decreased (P &lt; 0.05) in curcumin-supplemented groups. Total protein, albumin, globulin, HDL, growth hormone, T3, T4, TAC, CAT, SOD, IL -10, C3, and Lysosomes were significantly increased (P &lt; 0.05). At the same time, cholesterol, LDL, triglycerides and MDA, IL-1β, and IFN<strong>-γ</strong> levels were significantly reduced (P &lt; 0.05) by adding curcumin. Curcumin effectively reversed the heat stress-induced histopathological changes in the liver, spleen, bursa, intestine, and breast muscles. These results indicate that curcumin can mitigate the negative effects of heat stress on health and stimulate immunity and antioxidant status in broiler chickens.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"130 ","pages":"Article 104144"},"PeriodicalIF":2.9,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144138177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Thermal acclimatization mechanisms in the cold-water Ophiuroid Ophiura sarsii vadicola: Regulation of protein homeostasis and metabolic pathways","authors":"Xiaomei Liao ,&nbsp;Xinlong Li ,&nbsp;Anning Mou ,&nbsp;Qian Zhang ,&nbsp;Yue Dong ,&nbsp;Yixuan Li ,&nbsp;Xuelei Zhang ,&nbsp;Qinzeng Xu","doi":"10.1016/j.jtherbio.2025.104137","DOIUrl":"10.1016/j.jtherbio.2025.104137","url":null,"abstract":"<div><div>Global climate change is driving rapid warming in the Pacific Arctic cold-water regions. As dominant species in these areas, the <em>Ophiura sarsii</em> complex suffer from ocean warming stress, resulting in altered distribution patterns, physiological behaviors, and thermotolerance. However, their molecular response mechanisms to ocean warming remain unclear. We conducted transcriptomic analyses of <em>O. sarsii vadicola</em> under various temperature treatments: a control group at in-situ temperature (7 °C), two warming groups at the sub-lethal temperature 19 °C for one week (short-term) and two months (long-term), and a low-temperature reference group of <em>O. sarsii</em> at 0 °C. The results showed that a total of 66 core differentially expressed genes (58 up-regulated, 8 downregulated) were identified, with expression patterns varying by warming duration. <em>Ophiura sarsii vadicola</em> exhibited a more pronounced response to long-term warming than short-term ones. Three transcription factors <em>HMGB3</em>, <em>METTL19</em>, and <em>HEAT</em> were identified as key regulators within the co-expression networks. Furthermore, changes in folding enzymes activity and an upregulation of protein processing and chaperone genes were observed, indicating a potential activation of the unfolded protein response in brittle stars. Elevated temperatures also induced changes in multiple energy metabolism-related pathways. These findings suggest that <em>O. sarsii vadicola</em> may mitigate the negative effects of ocean warming by regulating key gene expression, enhancing protein homeostasis control and energy redistribution. This study provides valuable insights into the evolution and adaptability mechanisms of cold-water species under global climate change.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"130 ","pages":"Article 104137"},"PeriodicalIF":2.9,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144130874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reduced thermoregulatory ability contributes to increased mortality in urban box turtles (Terrapene spp.)","authors":"Stella F. Uiterwaal ,&nbsp;Jamie L. Palmer ,&nbsp;Saima Farook ,&nbsp;Sharon L. Deem ,&nbsp;Catherine Taylor ,&nbsp;Stephen Blake","doi":"10.1016/j.jtherbio.2025.104135","DOIUrl":"10.1016/j.jtherbio.2025.104135","url":null,"abstract":"<div><div>Urban wildlife often shows pronounced differences in ecology, behavior, and survival compared to rural individuals. Given the broad impacts of temperature in ecology, thermal discrepancies between cities and their rural surroundings are likely to play a major role in explaining these differences between urban and rural animals. Although experimental work has demonstrated the impacts of urban temperatures on wildlife, we lack field data on the body temperatures of free-living urban animals and the ecological impacts of these temperatures. In addition, while urban heat islands have directed research towards the impacts of warmer temperatures, relatively little focus has been given to the potential for urban organisms to experience colder temperatures. Here, we use 10 years of data to show that box turtles (<em>Terrapene</em> spp.) in an urban park are less effective thermoregulators than their rural counterparts at both hot and cold temperature extremes. In contrast to the heat island effect, the body temperatures of brumating urban box turtles are colder and more variable in the winter compared to rural turtles, despite similar environmental temperatures. Furthermore, during the hottest months we find no evidence of an urban heat island effect, yet body temperatures of urban turtles are hotter than rural turtles. These body temperature differences suggest that urban turtles have a reduced ability for behavioral thermoregulation, likely due to lower habitat quality and reduced movement capacity in the urban environment. We further show that turtles experiencing extremely cold body temperatures in the winter are less likely to survive the subsequent year, indicating that over-winter thermoregulatory ability could play an under-appreciated role in the fitness and abundance of urban ectotherms. As urbanization continues to degrade landscapes and as climate change increases the occurrence of temperature extremes, we highlight the need to understand the impacts of cold temperatures on wildlife in human-altered environments.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"130 ","pages":"Article 104135"},"PeriodicalIF":2.9,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144105898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biothermomechanical interactions in two-dimensional living tissue under Atangana–Baleanu fractional derivatives","authors":"Areej Almuneef ,&nbsp;Ibrahim Abbas ,&nbsp;Alaa A. El-Bary ,&nbsp;Zuhur Alqahtani","doi":"10.1016/j.jtherbio.2025.104140","DOIUrl":"10.1016/j.jtherbio.2025.104140","url":null,"abstract":"<div><div>This study investigates the impact of the Atangana-Baleanu (AB) fractional derivative in two-dimensional living tissue under pulse heat flux. Atangana-Baleanu (AB) derivatives with local and non-singular kernels are accounted for in the fractional-order formulation. Unlike classical models such as Pennes' equation, which assumes infinite thermal propagation speed, the proposed framework captures finite thermal and mechanical wavefronts. The half-space surface's border experiences a pulse-shaped heat flux, yet the edge is fixed in displacement and free of shear stress. The analytical solutions for the variables are derived using Laplace and Fourier transforms combined with the eigenvalue approach methodology. The numerical calculations have been done, and the finding are graphically displayed to estimate the impacts of fractional order parameter and heat flux pulse time in temperature distribution and mechanical responses. Numerical results indicate that thermal and mechanical waves propagate through living tissues over a finite distance, effectively addressing the unrealistic predictions of Pennes' model.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"130 ","pages":"Article 104140"},"PeriodicalIF":2.9,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144106013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive transcriptomic and proteomic analysis reveals biological changes and potential regulatory mechanisms of endothelial cells under heat stress conditions","authors":"Keran Jia ,&nbsp;Jie Zheng ,&nbsp;Saicong Hu ,&nbsp;Daxin Lei ,&nbsp;Mengyu Jiang ,&nbsp;Jiajia Zhang ,&nbsp;Mengge Cui ,&nbsp;Jia Wang ,&nbsp;Yan Cui ,&nbsp;Fukun Wang","doi":"10.1016/j.jtherbio.2025.104138","DOIUrl":"10.1016/j.jtherbio.2025.104138","url":null,"abstract":"<div><div>Endothelial cells are crucial for vascular function, and they respond to heat stress by altering gene and protein expression. This study investigated the impact of heat stress on endothelial cells using transcriptomic and proteomic analyses. Human umbilical vein endothelial cells (HUVECs) were exposed to heat stress at 43 °C for 6 h, resulting in significant changes in gene and protein expression patterns. In particular, ORAI1, a key regulator of intracellular Ca²⁺ levels, was significantly upregulated. The integration of transcriptomic and proteomic data revealed genes and proteins associated with endothelial cell damage and repair processes. Validation experiments confirmed the upregulation of ORAI1 and increased intracellular Ca²⁺ levels under heat stress. These findings enhance our understanding of endothelial cell responses to heat stress and suggest potential therapeutic targets for heat-related diseases.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"130 ","pages":"Article 104138"},"PeriodicalIF":2.9,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144116859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}