Journal of thermal biology最新文献

{"title":"A critical review on magnetic fluid hyperthermia treatment for cancers: Challenges and pathways from computation to clinic","authors":"Pratik Roy ,&nbsp;Ranjan Ganguly ,&nbsp;Nirmalendu Biswas","doi":"10.1016/j.jtherbio.2025.104284","DOIUrl":"10.1016/j.jtherbio.2025.104284","url":null,"abstract":"<div><div>Standard therapeutic practices for malignancies have often been limited by recurrent challenges, particularly in advanced-stage cancers or following prolonged treatment exposure. Hyperthermia treatment, in conjunction with standard therapies, has been found to yield favorable results. With rapid advancement in the research for the development and synthesis of superparamagnetic nanoparticles (SPMNP), Magnetic Fluid Hyperthermia (MFH) has established itself as a promising platform for therapeutic intervention in malignant tumors. However, the development of reliable and effective numerical models is a domain that demands extensive research to accurately evaluate the dosimetric data of SPMNP to be infused, its mass transfer to and distribution within the analyzed domain, its cytotoxic effects on biological systems, generated heat flux under the influence of an Alternating magnetic field (AMF), rise in temperature and heat transfer within the tissue and lastly, the resultant damage caused to both malignant and adjoining healthy tissues. With this backdrop, one cannot overemphasize the role of modelling, and <em>in vitro</em> and <em>in vivo</em> experiments involving MFH. For accurate characterization of MFH and effective treatment outcome estimation, it is important to take into consideration the salient tissue-specific physiological parameters. All these physiological parameters, which in general have non-linear behavior are of prime important in forecasting treatment outcomes and its damage potential to the adjoining healthy tissue, which in turn ensures the acceptability of the treatment as a mainstream clinical procedure. This article presents an in-depth review of the available various biocompatible SPMNPs and their injection strategies, and heating capacity under the influence of an AMF. It also discusses the different classical Fourier law-based bio heat transfer models that are used for numerical study to predict the temperature profile of the ROI, their dependency on various bio-physical parameters of the tissue as well as magneto-physical parameters of the SPMNPs and AMF, and the capability of the models to reliably estimate tissue damage. The hindrances faced by the treatment procedures towards converting MFH into mainstream clinical trials is also discussed with a futuristic vision of integrating artificial intelligence and machine learning approach towards optimizing the MFH parameters.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"133 ","pages":"Article 104284"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145222126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mitochondrial adaptations from heat acclimation – A narrative review","authors":"Marcos S. Keefe ,&nbsp;Danielle E. Levitt ,&nbsp;Heather L. Vellers ,&nbsp;Courteney L. Benjamin ,&nbsp;Yasuki Sekiguchi","doi":"10.1016/j.jtherbio.2025.104283","DOIUrl":"10.1016/j.jtherbio.2025.104283","url":null,"abstract":"<div><div>In recent decades, global temperature and humidity levels have surged. These increases in temperature and humidity are associated with higher risk of heat-related illnesses and impaired exercise performance, thereby prompting investigation into physiological responses to heat stress. Preconditioning strategies, including heat acclimation/acclimatization (HA), elicit physiological adaptations to enhance response to future heat exposures. Within HA research, an area of growing interest is examination of subcellular adaptations that contribute to whole-body acclimation, such as changes to/within mitochondria. External heat stress alters molecular pathways involved in mitochondrial biogenesis and bioenergetic function, but the relationship between these alterations and whole-body HA adaptations remains relatively unknown. Therefore, this review provides a detailed examination of the impact of HA on mitochondria across cell models, rodent models, and humans, linking these changes to exercise performance. Based on the current evidence, we propose a HA protocol aimed at promoting mitochondrial adaptations while maximizing traditional HA benefits. Lastly, we identify key areas for future research to further explore and enhance our understanding of mitochondrial responses to HA.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"133 ","pages":"Article 104283"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145191896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fractional-order bioheat modeling for enhanced prediction of tissue response in thermal therapies","authors":"Mohamed Hisham Fouad Aref ,&nbsp;Abdallah Abdelkader Hussein ,&nbsp;Yasser H. El-Sharkawy","doi":"10.1016/j.jtherbio.2025.104287","DOIUrl":"10.1016/j.jtherbio.2025.104287","url":null,"abstract":"<div><h3>Introduction</h3><div>Thermal ablation planning requires models that capture non-Fourier, memory-dependent heat transport in heterogeneous soft tissues. Classical Pennes’ formulations often misestimate temperature rise and damage extent under clinical heating protocols.</div></div><div><h3>Objective</h3><div>To experimentally validate a fractional-order extension of Pennes’ bioheat equation against <em>ex-vivo</em> thermographic data and quantify its predictive advantage over the classical model.</div></div><div><h3>Materials and methods</h3><div>We (i) measured thermal diffusivity (D), conductivity (k), and volumetric specific heat capacity (C_h) for 30 <em>ex-vivo</em> tissue samples (kidney, heart, liver) at room temperature (20–25 °C); (ii) performed controlled surface laser heating on liver samples with infrared thermography; and (iii) simulated temperature evolution with the classical (α = 1) and fractional (0 &lt; α &lt; 1) bioheat models. Agreement between measurements and simulations was assessed via mean absolute error (MAE), root-mean-square error (RMSE), residual analysis, and Bland–Altman plots.</div></div><div><h3>Results</h3><div>Across experiments, the fractional model reproduced the measured temperature trajectories with consistently lower MAE/RMSE than the classical model and reduced bias in Bland–Altman analysis. A 2D benchmark confirmed expected spatial gradients under fixed boundary conditions, while sensitivity analyses showed α controls the pace of thermal penetration and the extent of predicted thermal zones. Experimental results showed consistent thermal parameters across tissue types (<em>ρ</em> ≈ 1050 kg/m3, D ≈ 0.15 mm²/s and k ≈ 0.5 W/m °C), blood properties were characterized by <span><math><mrow><msub><mi>Ω</mi><mi>p</mi></msub></mrow></math></span> = 0.005 l/s, <span><math><mrow><msub><mi>ρ</mi><mi>b</mi></msub></mrow></math></span> = 1060 kg/m³, <span><math><mrow><msub><mi>T</mi><mi>a</mi></msub></mrow></math></span> = 37 °C, with metabolic heat generation estimated as <span><math><mrow><msub><mi>Q</mi><mi>m</mi></msub></mrow></math></span> = 33800 W/m³. Minor, statistically insignificant variations were observed in specific heat capacity (C_h ≈ 3.71–3.43 MJ/m³. K), which aligned well with numerical predictions.</div></div><div><h3>Conclusions</h3><div>Integrating experimentally measured tissue properties with fractional bioheat modeling improves quantitative prediction of temperature evolution during ablative heating. This hybrid framework strengthens treatment planning by better delineating thermal spread and offers a practical path to patient- and tissue-specific calibration.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"133 ","pages":"Article 104287"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145258563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of dopaminergic signaling in insect response to repeated acute heat and cold stress","authors":"Mariam Hakim ,&nbsp;Samar El-Kholy ,&nbsp;Amira El-Keredy ,&nbsp;Yahya Al Naggar","doi":"10.1016/j.jtherbio.2025.104285","DOIUrl":"10.1016/j.jtherbio.2025.104285","url":null,"abstract":"<div><div>As climate change accelerates, organisms face increasing exposure to unpredictable heat and cold extremes, threatening survival, reproduction, and long-term population stability. Understanding the physiological mechanisms underlying thermal resilience is essential to predict species responses and mitigate biodiversity loss. This study investigates the role of dopamine (DA) and its related receptors in mediating thermal tolerance and regulating life history traits in <em>Drosophila melanogaster</em>. Using wild-type Canton-S flies, we exposed adults to repeated acute thermal stress—daily 1-h cold (15 °C) or heat (35 °C) shocks for five to seven days—alongside a control group maintained at 25 °C. We assessed survival, fecundity, developmental time, adult emergence, and sex ratio. DA levels, measured by HPLC, significantly increased under heat stress and declined under cold exposure, suggesting its involvement in thermal response signaling. To further explore receptor-specific roles, we examined dopaminergic receptor mutants in Dop1R1, Dop1R2, Dop2R, DopEcR under the same thermal conditions described above. Our findings reveal that deficiencies in specific DA receptors markedly influenced survival and reproduction under thermal stress. For instance, Dop1R2 mutants exhibited high fecundity but poor survival under heat shock, while DopEcR mutants showed reduced fecundity under both cold and heat stress without affecting survival. Dop1R1 and Dop2R mutants exhibited developmental delays under thermal stress, indicating their contribution to life cycle regulation. These findings highlight dopaminergic signaling as a critical modulator of thermal adaptation, with implications for understanding how neurophysiological plasticity may buffer or limit insect resilience to future climate variability.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"133 ","pages":"Article 104285"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145199406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Thermal analysis of biological tissue under pulsed laser irradiation accounting for temperature-dependent properties","authors":"Yingze Wang,&nbsp;Zhaowei He,&nbsp;Sheng Zhang,&nbsp;Shuang Zeng","doi":"10.1016/j.jtherbio.2025.104279","DOIUrl":"10.1016/j.jtherbio.2025.104279","url":null,"abstract":"<div><div>This study proposes an analytical approach to examine the thermal response of biological tissues with temperature-dependent properties subjected to pulsed laser irradiation. A bio-heat conduction model incorporating variable thermo-physical parameters was developed based on the dual-phase lag (DPL) heat conduction theory. Using the Laplace transform technique, an exact solution of the governing equation was derived in the transform domain, while numerical solutions in the time domain were obtained through the Stehfest inversion algorithm. The temperature distributions in typical biological tissues under pulsed laser irradiation were systematically computed, and the influences of temperature-dependent thermal parameters, blood perfusion rate, and metabolic heat generation were quantitatively evaluated under distinct heating mechanisms governed by phase lag times. The results reveal significant variations in thermal response due to temperature dependence, offering valuable insights for optimizing thermal therapy protocols.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"133 ","pages":"Article 104279"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145186145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dilation of the distal convoluted tubule induced by repeated heat exposure in rats model","authors":"Zhongyou Li ,&nbsp;Taoping Bai ,&nbsp;Wentao Jiang ,&nbsp;Fei Yan ,&nbsp;Lingjun Liu ,&nbsp;Chong Chen","doi":"10.1016/j.jtherbio.2025.104277","DOIUrl":"10.1016/j.jtherbio.2025.104277","url":null,"abstract":"<div><div>Thermal therapy (TT) has developed as a nonpharmacological strategy for various diseases, but little is known about its impact on the kidneys. This study conducted an animal experiment to elucidate this impact. We randomized 12 rats to control and TT groups and subjected them to 24 °C and 41 °C, respectively, once a day for four weeks. We monitored blood component changes weekly and examined rat kidneys pathologically after the trial. Cutaneous flux and renal blood flow were assessed before and during the intervention. The cross-sectional area of the distal convoluted tubule (DCT) significantly increased in the TT group compared to the control group (P &lt; 0.001). The TT group's DCT showed an average perimeter elongation of 75.9 %, which is explained by mechanical strain and the proliferation of epithelial cells. Significant increases at 14 days (P &lt; 0.05) in blood urea nitrogen, creatinine, and uric acid levels indicated that renal damage was the cause of these occurrences. Furthermore, this damage is caused by thermally induced renal ischemia, as indicated by a 21.3 % decrease in mean blood flow in the renal artery, which was also evidenced by a 65.2 % increase in mean cutaneous flux and a significant rise in red blood cell count (P &lt; 0.05). Sirius red staining showed that there was no difference in fiber expression between the two groups. Although no progression of renal fibrosis was observed, repeated heat exposure could induce the dilation of DCT. These findings may guide the redesign of TT protocols for patients and recreational users.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"133 ","pages":"Article 104277"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145199441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Artificial intelligence-based multimodal framework for non-invasive detection of digital eye strain using thermal imaging and behavioral metrics","authors":"J. Persiya ,&nbsp;A. Sasithradevi","doi":"10.1016/j.jtherbio.2025.104280","DOIUrl":"10.1016/j.jtherbio.2025.104280","url":null,"abstract":"<div><div>Digital Eye Strain is an emerging occupational health concern with significant implications for digital well-being. To address the need for scalable and objective monitoring, EyeStrainNet is proposed. It is a multimodal and explainable health informatics framework that integrates thermal imaging and behavioral metrics for the non-invasive detection of Digital Eye Strain. Thermal images were captured pre- and post-screen exposure using a FLIR Edge Pro camera, and ocular temperature features were extracted from the inner and outer canthus and central cornea. Behavioral data, such as screen exposure duration and distraction levels, were recorded in parallel. A total of 197 samples (34 with significant strain, 163 without) were analyzed. Feature engineering and statistical analysis revealed strong correlations between ocular temperature changes and behavioral factors. The proposed EyeStrainNet, based on a one-dimensional convolutional neural network, was evaluated using 5-fold cross-validation. It achieved 97.5 % accuracy, 92.5 % precision, 94.3 % recall, 92.7 % F1-score, 99.7 % ROC-AUC, and 98.9 % PR-AUC, demonstrating strong performance with tight confidence intervals. EyeStrainNet outperformed baseline models such as One-Class SVM and XGBoost-SVM by 2–3 % in accuracy and 5–10 % in F1-score. SHAP-based explainability analysis identified temperature variation and distraction as dominant predictive features. This multimodal, explainable, and data-driven framework enables early-stage, non-clinical DES detection, promoting proactive digital wellness.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"133 ","pages":"Article 104280"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145222052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A preliminary investigation for the dietary inclusion of calcareous marine algae for grain-fed beef steers during a simulated heat wave","authors":"Stephanie L. Sammes ,&nbsp;John B. Gaughan ,&nbsp;Shane O'Connell ,&nbsp;Angela M. Lees","doi":"10.1016/j.jtherbio.2025.104291","DOIUrl":"10.1016/j.jtherbio.2025.104291","url":null,"abstract":"<div><div>This study investigated nutritional management strategies to reduce the impact of heat stress on feedlot steers. Twelve Angus steers (582 ± 8.92 kg LW) were housed in climate control rooms (<strong>CCR</strong>) for 21-days. Steers were randomly allocated to three treatments: 1) Control, fed a finisher diet (Control); 2) finisher diet to a heat load diet with 5 % additional roughage, from d4 until d16 (HL Ration); 3) finisher diet supplemented with calcareous marine algae (CMA; 70 g/steer/day Acid Buf, Celtic Sea Minerals), between d4 until d16. The 21-days consisted of three phases i) d0 to d6, Phase I, Temperature Humidity Index, THI 65 to 78; ii) d7 to d11 Phase II, THI 83 to 87); and iii) d12 to d20, Phase III, THI 65 to 78, for all steers. Cattle exhibited increased respiration rate (RR), panting score (PS) and rumen temperature (T_RUM) during Phase II (<em>P</em> &lt; 0.0001). During Phase II, dry matter intake (DMI) was approximately 5 kg lower when compared with Phase I. The diurnal rhythm in rumen pH and range in rumen pH differed during Phase II for both Control (<em>P</em> &lt; 0.0001) and CMA (<em>P</em> &lt; 0.0001), when compared with Phase I. Mean rumen pH was lower during Phase III compared with Phase I (<em>P</em> ≤ 0.002). During Phase II, Lipopolysaccharide binding protein (LBP) and Serum amyloid A (SAA) differed across the treatments, with CMA cattle exhibiting the lowest concentrations (<em>P</em> ≤ 0.04). Both Aspartate aminotransferase (AST) (P ≤ 0.009) and Glutamate dehydrogenase (GLDH) (P ≤ 0.004) were at a maximum during Phase III, with Control having greater AST (P ≤ 0.01) and GLDH (P ≤ 0.04). These results suggest that CMA supports DMI and markers of gut integrity, whilst potentially mediating liver damage, during heat wave events.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"133 ","pages":"Article 104291"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145318442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"METTL14 mediates SOCS1 mRNA m6A modification to regulate macrophage polarization to attenuate the mechanism of severe heat stroke lung injury","authors":"Peng Zhang ,&nbsp;Minghao He ,&nbsp;Wenfang Jiang ,&nbsp;Ling Ouyang ,&nbsp;Yang Gao ,&nbsp;Yonghua Li","doi":"10.1016/j.jtherbio.2025.104294","DOIUrl":"10.1016/j.jtherbio.2025.104294","url":null,"abstract":"<div><div>Severe heat stroke is an acute pathological state triggered by high temperature, in which lung injury is a major complication and an important cause of high mortality. RNA N6-methyladenosine modification can affect macrophage polarization by regulating the stability and translation of mRNAs, which plays an important role in inflammation and tissue repair.</div></div><div><h3>Methods</h3><div>Establishment of severe heatstroke (HS) mice. Assessment of lung tissue changes using hematoxylin and eosin staining and immunohistochemical methods. CD206/iNOS/DAPI staining was used to detect the M1/M2 ratio. Real-time PCR was used to determine gene expression. Protein expression was determined by ELISA, Western blotting. Establishment of a heatstroke model in MH-S alveolar macrophages. Transcriptome sequencing analysis was performed to screen for differential genes, as well as expression of related signaling pathways.</div></div><div><h3>Result</h3><div><em>In vivo</em> experiments have shown that severe pulmonary heatstroke caused an inflammatory response in lung tissue. RNA methylation modulators reduce lung tissue injury by decreasing macrophage expression while attenuating the expression of inflammatory factors. <em>In vitro</em> experiments showed that thermal shock significantly activated M1-type polarization in alveolar macrophages, induced activation of the NF-κB signaling pathway, and up-regulated the expression of the METTL14 gene and related inflammatory genes.</div></div><div><h3>Conclusion</h3><div>METTL14 significantly attenuates lung injury induced by severe heatstroke. This process is associated with its role in modulating the m6A modification of SOCS1 mRNA, which in turn affects the polarization state of macrophages and promotes the conversion from M1-to M2-type. This provides a new molecular target and theoretical basis for the treatment of severe heatstroke.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"133 ","pages":"Article 104294"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145308616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative acclimation capacity and thermal tolerance of a narrow endemic and a broadly distributed madtom catfish from the Ozark–Ouachita Mountain region, USA","authors":"Brittany L. McCall ,&nbsp;Orithea Z. Regn ,&nbsp;Brook L. Fluker","doi":"10.1016/j.jtherbio.2025.104295","DOIUrl":"10.1016/j.jtherbio.2025.104295","url":null,"abstract":"<div><div>Predicting the resilience of freshwater fishes to climate change requires understanding how species respond physiologically to elevated temperatures. We quantified upper thermal tolerance, as critical thermal maxima (CT_max), and acclimation capacity for two ecologically similar madtom catfishes with contrasting geographic distributions - the narrowly endemic and imperiled Caddo Madtom (<em>Noturus taylori</em>) and the broader distributed Black River Madtom (<em>Noturus maydeni</em>). Individuals of each species were acclimated to five temperatures (16, 19, 22, 25, and 28 °C) before CT_max determination. Both species exhibited significant increases in CT_max with higher acclimation temperatures, but <em>N. taylori</em> consistently maintained higher CT_max values (<em>F</em>_<em>4</em> = 11.14, <em>p</em> &lt; 0.001) and a greater acclimation response ratio (ARR = 0.50) compared to <em>N. maydeni</em> (ARR = 0.36). The thermal safety margin (TSM) declined with increasing acclimation temperature in both species (<em>F</em>_<em>4</em> = 408.60, <em>p</em> &lt; 0.001), though <em>N. taylori</em> retained a larger thermal buffer (<em>F</em>_<em>1</em> = 7.21, <em>p</em> = 0.01). Body size and condition changed during captivity, but these factors did not influence their thermal tolerance. Results indicate <em>N. taylori</em> may be more resilient to acute warming events than <em>N. maydeni</em>, though its restricted range and habitat specialization could limit behavioral mitigation options under sustained warming. These findings underscore the importance of integrating physiological and ecological data when assessing climate vulnerability in imperiled freshwater fishes.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"133 ","pages":"Article 104295"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145318489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}