{"title":"反复热暴露致大鼠远曲小管扩张模型。","authors":"Zhongyou Li, Taoping Bai, Wentao Jiang, Fei Yan, Lingjun Liu, Chong Chen","doi":"10.1016/j.jtherbio.2025.104277","DOIUrl":null,"url":null,"abstract":"<p><p>Thermal therapy (TT) has developed as a nonpharmacological strategy for various diseases, but little is known about its impact on the kidneys. This study conducted an animal experiment to elucidate this impact. We randomized 12 rats to control and TT groups and subjected them to 24 °C and 41 °C, respectively, once a day for four weeks. We monitored blood component changes weekly and examined rat kidneys pathologically after the trial. Cutaneous flux and renal blood flow were assessed before and during the intervention. The cross-sectional area of the distal convoluted tubule (DCT) significantly increased in the TT group compared to the control group (P < 0.001). The TT group's DCT showed an average perimeter elongation of 75.9 %, which is explained by mechanical strain and the proliferation of epithelial cells. Significant increases at 14 days (P < 0.05) in blood urea nitrogen, creatinine, and uric acid levels indicated that renal damage was the cause of these occurrences. Furthermore, this damage is caused by thermally induced renal ischemia, as indicated by a 21.3 % decrease in mean blood flow in the renal artery, which was also evidenced by a 65.2 % increase in mean cutaneous flux and a significant rise in red blood cell count (P < 0.05). Sirius red staining showed that there was no difference in fiber expression between the two groups. Although no progression of renal fibrosis was observed, repeated heat exposure could induce the dilation of DCT. These findings may guide the redesign of TT protocols for patients and recreational users.</p>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"133 ","pages":"104277"},"PeriodicalIF":2.9000,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Dilation of the distal convoluted tubule induced by repeated heat exposure in rats model.\",\"authors\":\"Zhongyou Li, Taoping Bai, Wentao Jiang, Fei Yan, Lingjun Liu, Chong Chen\",\"doi\":\"10.1016/j.jtherbio.2025.104277\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Thermal therapy (TT) has developed as a nonpharmacological strategy for various diseases, but little is known about its impact on the kidneys. This study conducted an animal experiment to elucidate this impact. We randomized 12 rats to control and TT groups and subjected them to 24 °C and 41 °C, respectively, once a day for four weeks. We monitored blood component changes weekly and examined rat kidneys pathologically after the trial. Cutaneous flux and renal blood flow were assessed before and during the intervention. The cross-sectional area of the distal convoluted tubule (DCT) significantly increased in the TT group compared to the control group (P < 0.001). The TT group's DCT showed an average perimeter elongation of 75.9 %, which is explained by mechanical strain and the proliferation of epithelial cells. Significant increases at 14 days (P < 0.05) in blood urea nitrogen, creatinine, and uric acid levels indicated that renal damage was the cause of these occurrences. Furthermore, this damage is caused by thermally induced renal ischemia, as indicated by a 21.3 % decrease in mean blood flow in the renal artery, which was also evidenced by a 65.2 % increase in mean cutaneous flux and a significant rise in red blood cell count (P < 0.05). Sirius red staining showed that there was no difference in fiber expression between the two groups. Although no progression of renal fibrosis was observed, repeated heat exposure could induce the dilation of DCT. These findings may guide the redesign of TT protocols for patients and recreational users.</p>\",\"PeriodicalId\":17428,\"journal\":{\"name\":\"Journal of thermal biology\",\"volume\":\"133 \",\"pages\":\"104277\"},\"PeriodicalIF\":2.9000,\"publicationDate\":\"2025-09-24\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of thermal biology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1016/j.jtherbio.2025.104277\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of thermal biology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1016/j.jtherbio.2025.104277","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOLOGY","Score":null,"Total":0}
Dilation of the distal convoluted tubule induced by repeated heat exposure in rats model.
Thermal therapy (TT) has developed as a nonpharmacological strategy for various diseases, but little is known about its impact on the kidneys. This study conducted an animal experiment to elucidate this impact. We randomized 12 rats to control and TT groups and subjected them to 24 °C and 41 °C, respectively, once a day for four weeks. We monitored blood component changes weekly and examined rat kidneys pathologically after the trial. Cutaneous flux and renal blood flow were assessed before and during the intervention. The cross-sectional area of the distal convoluted tubule (DCT) significantly increased in the TT group compared to the control group (P < 0.001). The TT group's DCT showed an average perimeter elongation of 75.9 %, which is explained by mechanical strain and the proliferation of epithelial cells. Significant increases at 14 days (P < 0.05) in blood urea nitrogen, creatinine, and uric acid levels indicated that renal damage was the cause of these occurrences. Furthermore, this damage is caused by thermally induced renal ischemia, as indicated by a 21.3 % decrease in mean blood flow in the renal artery, which was also evidenced by a 65.2 % increase in mean cutaneous flux and a significant rise in red blood cell count (P < 0.05). Sirius red staining showed that there was no difference in fiber expression between the two groups. Although no progression of renal fibrosis was observed, repeated heat exposure could induce the dilation of DCT. These findings may guide the redesign of TT protocols for patients and recreational users.
期刊介绍:
The Journal of Thermal Biology publishes articles that advance our knowledge on the ways and mechanisms through which temperature affects man and animals. This includes studies of their responses to these effects and on the ecological consequences. Directly relevant to this theme are:
• The mechanisms of thermal limitation, heat and cold injury, and the resistance of organisms to extremes of temperature
• The mechanisms involved in acclimation, acclimatization and evolutionary adaptation to temperature
• Mechanisms underlying the patterns of hibernation, torpor, dormancy, aestivation and diapause
• Effects of temperature on reproduction and development, growth, ageing and life-span
• Studies on modelling heat transfer between organisms and their environment
• The contributions of temperature to effects of climate change on animal species and man
• Studies of conservation biology and physiology related to temperature
• Behavioural and physiological regulation of body temperature including its pathophysiology and fever
• Medical applications of hypo- and hyperthermia
Article types:
• Original articles
• Review articles