Journal of thermal biology最新文献

{"title":"Deciphering the molecular mechanisms of heat stress tolerance in goats: Insights from transcriptome and Gene Co-expression analysis","authors":"Mahesh Shivanand Dige ,&nbsp;Ankita Gurao ,&nbsp;Arnav Mehrotra ,&nbsp;Manoj Kumar Singh ,&nbsp;Amit Kumar ,&nbsp;Rakesh Kaushik ,&nbsp;Ranjit Singh Kataria ,&nbsp;Pramod Kumar Rout","doi":"10.1016/j.jtherbio.2024.104007","DOIUrl":"10.1016/j.jtherbio.2024.104007","url":null,"abstract":"<div><div>Climate change poses a significant threat to the sustainability of livestock production systems in developing countries, particularly impacting small ruminants like goats, which are highly susceptible to heat stress. This stressor not only reduces productivity but also undermines economic viability. This study aimed to delve into the molecular mechanisms underlying heat stress tolerance in goats by conducting a comprehensive transcriptome analysis of heat-tolerant (HT, n = 4) and heat-susceptible (HS, n = 6) Jamunapari goats. Physiological metrics, such as rectal temperature, respiratory rate, and heart rate, were meticulously monitored under extreme environmental conditions (Temperature Humidity Index &gt;92) to effectively classify goats based on their distinct heat stress responses. Samples of blood were obtained, and peripheral blood mononuclear cells (PBMCs) were extracted for subsequent RNA extraction. RNA-Seq analysis revealed a sum of 734 differentially expressed genes (DEGs), comprising 251 upregulated and 483 downregulated genes in HT goats compared to their HS counterparts. The WGCNA revealed three key modules, darkorange (tolerance), paleturquoise (respiration rate), and darkmagenta (heart rate). Moreover, functional enrichment analysis revealed that DEGs within these modules played intricate roles in crucial biological processes and pathways, including mitochondrial function, cardiac function, immune response, genomic stability, and metabolic regulation. This research notably enhances our comprehension of the genetic underpinnings of thermo-tolerance in goats and provides invaluable guidance for formulating breeding strategies aimed at bolstering livestock resilience against the challenges of climate change.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142569041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Life stage-specific effects of heat stress on spermatogenesis and oogenesis in Drosophila melanogaster","authors":"Abhishek Meena,&nbsp;Komal Maggu,&nbsp;Alessio N. De Nardo,&nbsp;Sonja H. Sbilordo,&nbsp;Benjamin Eggs,&nbsp;Rawaa Al Toma Sho,&nbsp;Stefan Lüpold","doi":"10.1016/j.jtherbio.2024.104001","DOIUrl":"10.1016/j.jtherbio.2024.104001","url":null,"abstract":"<div><div>Biodiversity is increasingly threatened by unpredictable, frequent, and intense climatic events like heatwaves that pose harmful impacts on ectotherms. Beyond the health and survival of organisms, reduced reproductive performance has emerged as a critical fitness consequence of thermal stress induced by high temperatures. Many studies on these effects expose organisms to heat stress during the adult stage or throughout development, often focusing on cumulative effects across life stages, and they tend to examine one or the other sex. This approach may not reflect the short-term nature of many extreme heat events and limits our understanding of stage- and sex-specific fitness consequences in short-lived organisms. To address this gap, we used <em>Drosophila melanogaster</em> to investigate the sex-specific reproductive performance following short heat stress of varying intensity at different developmental stages. We found the thermal sensitivity to be higher in males than females, and to increase toward adult emergence, leading to nearly complete reproductive failure and substantially slowed recovery. These results highlight how even brief bouts of heat stress during a sensitive phase could affect population dynamics and persistence. Our findings also underscore that incorporating both sex and life stage could improve predictions of species persistence.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142560595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Five weeks of intermittent hand exposures to alternating cold and hot stimuli do not modify finger cold-induced vasodilatation response","authors":"Roger Kölegård ,&nbsp;Lena Norrbrand ,&nbsp;Ola Eiken ,&nbsp;Michail E. Keramidas","doi":"10.1016/j.jtherbio.2024.104004","DOIUrl":"10.1016/j.jtherbio.2024.104004","url":null,"abstract":"<div><div>We tested the hypothesis that prolonged intermittent hand exposures to transient contrast thermal stimuli would enhance the finger cold-induced vasodilatation (CIVD) response during localized cooling. Eight healthy men participated in a 5-week regimen, during which they immersed, thrice per week, the non-dominant (EXP) hand in 8° and 43 °C water, sequentially and at 3-min intervals, for a total period of 60 min. The contralateral (i.e., dominant) hand served as the control (CON) hand. Before and after the regimen, subjects conducted two 30-min hand cold (8 °C water) provocation trials, one with the EXP hand and the other with the CON hand. In addition, a flow-mediated dilatation test was performed in the brachial artery of the EXP arm. Regardless of the hand tested, the average finger skin temperature [CON hand: pre-trial = 10.5 (1.2)°C, post-trial = 10.8 (1.3)°C and EXP hand: pre-trial = 10.7 (1.1)°C, post-trial 10.9 (1.1)°C; <em>p</em> = 0.79], and the incidence of CIVD events [CON hand: pre-trial = 1.1 (1.2) events, post-trial = 1.2 (1.1) events and EXP hand: pre-trial = 1.1 (0.8) events, post-trial = 1.1 (0.8) events; <em>p</em> = 0.88] were not affected by the 5-week regimen. The sensation of cold-induced pain was transiently alleviated following the regimen (<em>p</em> = 0.02). The flow-mediated dilatation response of the EXP brachial artery remained unaltered [pre-trial = 5.4 (3.2)%, post-trial = 4.7 (3.6)%; <em>p</em> = 0.51]. Therefore, five weeks of intermittent hand exposures to alternating cold and hot stimuli do not improve finger temperature responsiveness to sustained localized cold.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142564331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of acute mild winter conditions on the productivity of feedlot cattle: An Australian perspective","authors":"Pippa J. Pryor ,&nbsp;Billie Standfield ,&nbsp;Janelle Wilkes,&nbsp;Léa Labeur ,&nbsp;Angela M. Lees","doi":"10.1016/j.jtherbio.2024.103989","DOIUrl":"10.1016/j.jtherbio.2024.103989","url":null,"abstract":"<div><div>The impact of cold stress on feedlot cattle has received limited investigation in temperate climates. However, cold stress has been found to impact cattle welfare and production in temperate climatic conditions, where ambient temperature (T_A), precipitation, relative humidity (RH), wind speed (WS) and solar radiation (SR) all influencing apparent temperature. While there are many indices to quantify cold stress in cattle, there are limited indices that directly relate to cattle and account for all the interactions between climatic variables. This makes cold stress difficult to quantify, with on-cattle measures such as core body temperature and shivering difficult metrics to obtain in feedlot cattle. Moreover, individual cattle will react differently to cold conditions based on their production, age, sex, coat characteristics (colour and type), breed and nutrition. While cold stress can be difficult to measure, the production status, behavioural and physiological effects of cold stress have been identified, but generally under what could be considered as extreme cold stress events. Similarly, while mitigation strategies such as shelter, bedding and windbreaks have been investigated, limited information is available surrounding their usefulness in temperate climates. Further research is needed to fully investigate the effects of cold stress and how to mitigate those effects in feedlot cattle under conditions, such as those found in Australia.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142365715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Patterns of thermal normality of the knee joint in professional athletes of different sports","authors":"Cristiane Mara de Rezende ,&nbsp;Alisson Gomes da Silva ,&nbsp;Manuel Sillero-Quintana ,&nbsp;João Carlos Bouzas Marins","doi":"10.1016/j.jtherbio.2024.103991","DOIUrl":"10.1016/j.jtherbio.2024.103991","url":null,"abstract":"","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142468720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Temperature as an effective biosecurity tool against invasive snakes","authors":"Julien C. Piquet ,&nbsp;Borja Maestresalas ,&nbsp;Marta López-Darias","doi":"10.1016/j.jtherbio.2024.103978","DOIUrl":"10.1016/j.jtherbio.2024.103978","url":null,"abstract":"<div><div>The application of thermal treatments could serve as a biosecurity tool to avert snake introduction, which often leads to irreversible ecological impacts. Here, we tested the applicability of conductive heating and thermal fumigation to prevent the spread of the California kingsnake (<em>Lampropeltis californiae</em>), a damaging species established on the island of Gran Canaria, likely to reach other vulnerable regions, and included among the most concerning invasive species of the European Union. We exposed 24 individuals to a thermal gradient to determine species selected temperatures, range of preferred temperatures and voluntary thermal maximum and used other 24 individuals to analyse their response to conductive heating, thermal fumigation and control (no heat) treatments. <em>Lampropeltis californiae</em> selected temperatures of 27.80 ± 1.05 °C (ranging 26.86 °C ± 1.21 °C to 28.68 °C ± 1.25 °C) and a voluntary thermal maximum of 32.50 ± 3.69 °C. Conductive heating and thermal fumigation performed equally well, inducing the exit of 83.33% and 91.67% of all individuals after 14.36 ± 9.25 min and 11.13 ± 8.60 min of exposition, and at a body temperature of 35.66 ± 3.53 °C and 35.57 ± 2.41 °C, respectively. Control treatments produced the exit of 29.17% of all individuals, which came out of the box in 24.80 ± 8.83 min and at a body temperature of 28.60 ± 1.38 °C. Thermal treatments could serve as an effective tool to prevent the inadvertent transportation of <em>L. californiae</em> and other invasive snakes threatening numerous regions around the globe.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142377982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adaptive capability of slow-growing backyard poultry as indicated by physiological and molecular responses in a hot and humid coastal climate","authors":"Nibedita Nayak ,&nbsp;Subrat Kumar Bhanja ,&nbsp;Eaknath B. Chakurkar ,&nbsp;Amiya Ranjan Sahu","doi":"10.1016/j.jtherbio.2024.103985","DOIUrl":"10.1016/j.jtherbio.2024.103985","url":null,"abstract":"<div><div>Assessing the adaptability of slow-growing rural chickens for improving thermotolerance to suit the global climate change is a major research need. This work was aimed to evaluate the adaptability of CARI-Debendra chickens and to identify the polymorphism as well as expression profiling of thermotolerant genes (HSP70 and GRP78) under prevailing temperature-humidity indices and thermal stress in a coastal environment. One hundred sixty straight run chicks were reared at THI≥75 (control) and THI&gt;80 under coastal climate till 12 weeks. Polymorphism of HSP70 and GRP78 candidate genes were explored using restriction enzymes TaqI and HaeIII to identify possible thermotolerance markers. Expression profiling of both the genes in liver, intestine and pectoralis muscle was determined through quantitative real-time PCR. Rectal and body surface temperature recorded in the neck and back showed significant differences (P &lt; 0.01) with higher temperature in THI&gt;80 group. Comparatively lower live weights (P &lt; 0.05) and poor FCR were recorded in THI&gt;80 group. The villi height in all intestinal segments was significantly lower (P &lt; 0.01), but deeper crypt depth was observed in THI&gt;80 than control group. A lowest thymus weight (P &lt; 0.05) was noted with no significant differences in immune response in treatment group. Serum levels of cholesterol, activities of lactate dehydrogenase, creatinine kinase and concentration of potassium, sodium and thyroxine hormone were not different between the 2 groups. The concentration of triiodothyronine and chloride ion was lower in THI&gt;80 group indicating adaptive changes for thermoregulation. HSP70 gene expressions in the three tissues were differentially increased (P &lt; 0.01) by temperature-humidity indices, but the expression of GRP78 was not different between the 2 groups. The results concluded that the environmental factors interact with genetics on adaptability towards thermotolerance in slow-growing chickens.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142377981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cardiac electrical and functional activity following an outdoor cold-water swimming event","authors":"Victorien Faivre-Rampant ,&nbsp;Corentin Hingrand ,&nbsp;A. Mezanger ,&nbsp;Eric Saloux ,&nbsp;Pierre Ollitrault ,&nbsp;Simon Alvado ,&nbsp;Hervé Normand ,&nbsp;Igor B. Mekjavic ,&nbsp;Thibaud Collet ,&nbsp;Benoit Mauvieux ,&nbsp;Joffrey Drigny ,&nbsp;Amir Hodzic","doi":"10.1016/j.jtherbio.2024.103996","DOIUrl":"10.1016/j.jtherbio.2024.103996","url":null,"abstract":"<div><h3>Aims</h3><div>Participation in outdoor cold-water swimming (OCWS) events combines endurance exercise and cold exposure. Concerns have emerged about the potential risk of acute adverse cardiac events during OCWS, particularly during endurance events. We analysed the effect of prolonged OCWS on cardiac function in trained athletes.</div></div><div><h3>Methods</h3><div>The swimming event consisted of laps over a 1000-m course, for up to 6 h, in water at 15 °C. Twenty participants (11 males, 47.3 ± 8.6 years old) were included. Core temperature (T_core) was monitored using an ingestible temperature sensor during and up to 1 h after the swim. Body composition, blood pressure, electrocardiogram (ECG), and transthoracic echocardiography were assessed 1 day before the event and within the first hour upon completion of the swim.</div></div><div><h3>Results</h3><div>Mean body mass index was 27.1 ± 5.1 kg/m² and fat mass was 25.2 ± 9.1 %. Mean duration of swimming was 214 ± 115 min. Minimum Tcore was 35.6 ± 1.3 °C. A significant lengthening of the QT interval corrected (QTc) for heart rate was observed post-exercise (437.7 ± 27.7 vs. 457.2 ± 35.9 ms, p = 0.012), with 5 participants exhibiting post-exercise QTc &gt;500ms. OCWS did not alter the biventricular systolic function and left ventricular relaxation. No correlation was observed between ΔQTc and ΔTcore.</div></div><div><h3>Conclusion</h3><div>OCWS seemed to acutely delay post-exercise cardiac repolarization without alteration of cardiac function in a healthy trained population. Additional investigations would be warranted to explore the clinical implications of QT lengthening and its relationship with autonomic nervous system regulation during OCWS.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142502914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plastic use as nesting material can alter incubation temperature and behaviour but does not affect yellow-legged gull chicks","authors":"Sara N. Veríssimo ,&nbsp;Filipe Veloso ,&nbsp;Francisco Neves ,&nbsp;Jaime A. Ramos ,&nbsp;Vitor H. Paiva ,&nbsp;Ana C. Norte","doi":"10.1016/j.jtherbio.2024.104005","DOIUrl":"10.1016/j.jtherbio.2024.104005","url":null,"abstract":"<div><div>Optimal incubation temperature is crucial for embryos' development and survival. With the increasing use of plastics in gulls' nests, it is essential to understand how their incorporation affects incubation temperature, parental behaviour, and hatching success. Considering this, we conducted an experiment where plastic was introduced into yellow-legged gulls (<em>Larus michahellis</em>) nests. The experiment comprised three groups: a control group, a group with low amount of plastic, and a third with a high amount of plastic. This design allowed us to investigate the effects of plastic on 1) the heart rate of incubating adults, 2) the number and duration of adults' absences from their nest, 3) how the presence or absence of the adult influenced egg temperature, and 4) chick hatching success, physiological parameters, and bill colour phenotype. We observed that incubation temperature was consistently higher in nests with plastic. The number of absences was higher in the low plastic group at increased temperatures, though the duration was significantly lower in both plastic groups than in the control, possibly to mitigate the effects of heat stress. During higher environmental temperatures, heart rate was higher for the high plastic group. The increase in heart rate in the low plastic group was less pronounced with increasing environmental temperatures. No significant effects were observed on hatching success or in the health condition of young chicks, except for high values of haemoglobin in both plastic groups, which might indicate dehydration.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142553693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Thermal perception and dressing behaviors of pregnant women during different stages of pregnancy: A field study at a hospital in Xi'an, China","authors":"Yi Xu ,&nbsp;Yue Li ,&nbsp;Lijuan Wang ,&nbsp;Ji Yang ,&nbsp;Hui Zhang","doi":"10.1016/j.jtherbio.2024.104002","DOIUrl":"10.1016/j.jtherbio.2024.104002","url":null,"abstract":"<div><div>Pregnant women have different thermal preferences during pregnancy. This paper aims to investigate the thermal characteristics of pregnant women in hospitals, addressing a research gap in the field of thermal comfort for this population. This study assessed the thermal environments of the pregnancy examination spaces (i.e., B-ultrasound room and waiting area) in a hospital located in Xi'an during the winter season. Data were collected from 1055 pregnant women at different stages of pregnancy, including demographic characteristics, thermal sensation votes (TSVs), thermal comfort votes (TCVs), and clothing insulation. The results show that the thermal sensation was significantly more influenced by the pregnancy stage than the current weight. Pregnant women in the third trimester reported a higher mean thermal sensation vote (MTSV) than in other pregnancy stages. Furthermore, the clothing insulation of pregnant women exhibited a non-normal distribution, resembling a bimodal structure. Based on this structure, typical clothing insulation values were determined to be 0.9 clo in the B-ultrasound room and 1.3 clo in the waiting area. Given these characteristics in both thermal perception and dressing behaviors of pregnant women via a subjective questionnaire, we advise lowering the temperature to ensure their comfort and save heating energy. Some personalized seats are also provided to compensate for differences in thermal preference at various stages of pregnancy. These findings contribute to the creation of comfortable and healthy pregnancy examination environments.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142577774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}