Journal of thermal biology最新文献

{"title":"Inhibitory effect of SMAD2 on myogenesis in heat-stressed Hu sheep myoblasts","authors":"Jiawei Lu,&nbsp;Yilan Liu,&nbsp;Huixia Li","doi":"10.1016/j.jtherbio.2025.104223","DOIUrl":"10.1016/j.jtherbio.2025.104223","url":null,"abstract":"<div><div>Heat stress can cause muscle damage and affect myogenesis. Myogenesis is a complicated process modulated by numerous genes. While SMAD2 has been implicated in various biological processes, its specific role in the myogenesis of heat-stressed Hu sheep myoblasts remains unclear. To explore the function of <em>SMAD2</em> in myogenesis, si-SMAD2-446 and pcDNA3.1-SMAD2 were transfected into heat-stressed Hu sheep myoblasts. The results indicated that heat stress increased the SMAD2 expression in Hu sheep myoblasts. Furthermore, <em>SMAD2</em> was found to suppress proliferation in heat-stressed Hu sheep myoblasts by downregulating the expression of proliferation-related genes, including proliferating cell nuclear antigen (PCNA), cyclin D1 (CCND1) and cyclin-dependent kinase 2 (CDK2). Additionally, <em>SMAD2</em> inhibited differentiation by downregulating the expression of myogenic differentiation factor (MYOD) and myogenin (MYOG). Moreover, <em>SMAD2</em> promoted apoptosis by activating BCL-associated X protein (BAX), B-cell CLL/lymphoma 2 (BCL-2), Caspase3 and Caspase9 in heat-stressed Hu sheep myoblasts. In conclusion, these findings suggest that <em>SMAD2</em> functions as an inhibitory factor in the myogenesis of heat-stressed Hu sheep myoblasts.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"132 ","pages":"Article 104223"},"PeriodicalIF":2.9,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144890262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Thermoregulation strategies differ for large macropods during high temperatures","authors":"Loic Q. Juillard,&nbsp;Lisa M. Ashby,&nbsp;Taylor J. McEvoy,&nbsp;Daniel Ramp","doi":"10.1016/j.jtherbio.2025.104245","DOIUrl":"10.1016/j.jtherbio.2025.104245","url":null,"abstract":"<div><div>In semi-arid drylands, landscape features such as water and trees are vital for individuals when reducing heat stress. In Australia, such landscapes have witnessed widespread canopy loss and considerable shifts in water availability due to anthropogenic processes, and are subject to greater frequencies of extreme temperatures. We explored the use of dammed watercourses and excavated earth tanks, and tree shade in two large macropod species, eastern grey kangaroos (<em>Macropus giganteus</em>) and red kangaroos (<em>Osphranter rufus</em>) in the semi-arid drylands of south-western Queensland. Using a thermal drone, camera traps, and temperature sensors, we examined the relationship between temperature, and water and canopy shade use by both species. The likelihood of kangaroos being observed in the sun was negatively correlated with temperature, with the likelihood of observing eastern grey kangaroos in the sun dropping below 50 % when temperatures exceeded 28 °C, and 17 °C for red kangaroos. Probability of detecting kangaroos in the shade was positively correlated with temperature, with red kangaroos more strongly selecting shade than eastern grey kangaroos. For eastern grey kangaroos, we observed greatly increased activity at waterpoints when daily maximum temperatures exceeded 28 °C, with a preference for dammed watercourses over excavated earth tanks. Only a weak trend of using dammed watercourses at high temperatures (&gt;36 °C) was detected for red kangaroos. As higher temperatures become more frequent due to climate change, our results suggest that the capacity of wildlife to persist may increasingly depend on the provision and maintenance of landscape features such as water and canopy shade.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"132 ","pages":"Article 104245"},"PeriodicalIF":2.9,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144886255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Erratum to “A phenological shift to save the boys? Current and projected trends of hatchling sex ratio of the loggerhead turtle Caretta caretta at Dalyan Beach, Türkiye” [J. Therm. Biol. 129 (2025) 104091]","authors":"Jay Kirkham ,&nbsp;Dogan Sözbilen ,&nbsp;Arzu Kaska ,&nbsp;Çisem Sezgin ,&nbsp;Yakup Kaska","doi":"10.1016/j.jtherbio.2025.104129","DOIUrl":"10.1016/j.jtherbio.2025.104129","url":null,"abstract":"","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"132 ","pages":"Article 104129"},"PeriodicalIF":2.9,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144847176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intracerebroventricular injection of taurine affords thermotolerance and regulates mitochondrial thermogenic gene expression in pectoralis muscle in neonatal chicks exposed to high ambient temperature","authors":"Mohamed Z. Elhussiny ,&nbsp;Haruka Nishimura ,&nbsp;Shogo Haraguchi ,&nbsp;Elizabeth R. Gilbert ,&nbsp;Mark A. Cline ,&nbsp;John F. Cockrem ,&nbsp;Eiki Takahashi ,&nbsp;Yoshimitsu Ouchi ,&nbsp;Takashi Bungo ,&nbsp;Vishwajit S. Chowdhury","doi":"10.1016/j.jtherbio.2025.104238","DOIUrl":"10.1016/j.jtherbio.2025.104238","url":null,"abstract":"<div><div>Intracerebroventricular (ICV) injection of taurine can induce hypothermia and attenuate stress behaviors in neonatal chicks under control thermoneutral temperature (CT) conditions. While, its ability to withstand heat stress (thermotolerance) has not identified yet. Thus, the objective of this study was to examine the effects of ICV taurine effects on thermoregulation, mitochondrial thermogenic gene expression, and amino acid metabolism in 5-day-old Julia male chick exposed to either high ambient temperature <strong>(</strong>HT<strong>)</strong> or CT conditions. Procedures were performed in the absence of food with water available after injections. The results showed that ICV injection of taurine reduced the magnitude of temperature rises in heat-exposed chicks (afforded thermotolerance). Central taurine increased gene expression of avian uncoupling protein mRNA in pectoral muscle under CT and HT. However, avian peroxisome proliferator-activated receptor γ coactivator-1α, avian adenine nucleotide translocase and carnitine palmitoyltransferase-I mRNA were decreased in chicks that were administered taurine under CT and HT. Diencephalic tryptophan, taurine and 3-methylhistidine were increased, while glutamic acid was decreased following taurine treatment under both CT and HT. Moreover, central taurine caused to reduce plasma triacylglycerol and uric acid concentrations. In conclusion, central taurine administration reduced temperature rises and was associated with changes in gene expression of mitochondrial thermogenic factors in heat-exposed fasted chicks. The changes in the mitochondrial thermogenic genes in muscle and amino acid concentrations in the brain suggest that skeletal muscle and central nervous system metabolism may be involved in the effects of taurine on thermoregulation in chicks under both CT and HT.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"132 ","pages":"Article 104238"},"PeriodicalIF":2.9,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144916267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Response of rice flower carp (Cyprinus carpio var.) to high-temperature stress: Mechanisms and limits of tolerance","authors":"Chunxing Cheng ,&nbsp;Wenfei Tian ,&nbsp;Jinyou Wei ,&nbsp;Linli Li ,&nbsp;Haizhen Tan ,&nbsp;Zhengjun Wu ,&nbsp;Jiaoyun Jiang","doi":"10.1016/j.jtherbio.2025.104244","DOIUrl":"10.1016/j.jtherbio.2025.104244","url":null,"abstract":"<div><div>Climate change presents a considerable challenge to the sustainable development of aquaculture. Research on the impact of high-temperature stress on rice flower carp (<em>Cyprinus carpio</em> var.) is still limited. In this study, rice flower carp (<em>Cyprinus carpio</em> var.) were exposed to four temperature treatments for 14 days: CK (control, 26 °C), TL (low temperature, 28 °C), TM (medium temperature, 30 °C), and TH (high temperature, 32 °C). The findings revealed that high temperatures significantly influenced the diversity of gut microbiota. Moreover, high-temperature stress disrupted the structural integrity of intestinal and liver tissues, causing notable damage. It also impaired the antioxidant system, leading to the accumulation of malondialdehyde (MDA) and inducing oxidative stress. At the transcriptional level, differentially expressed genes (DEGs) associated with the protein processing pathway (e.g., <em>HSP40</em>, <em>HSP70</em>, <em>Bip</em>) and the PPAR (peroxisome proliferator-activated receptor) signaling pathway (e.g., <em>PPARδ</em>, <em>CPT1</em>) were significantly upregulated during temperature acclimation. However, further temperature increases inhibited their expression, highlighting their crucial roles in the high-temperature acclimation of rice flower carp. In conclusion, rice flower carp displayed a degree of tolerance to temperatures up to 30 °C. Beyond this threshold, their health and physiological functions were adversely affected. Our study provides a valuable reference for understanding the impact of high-temperature stress on aquatic animals.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"132 ","pages":"Article 104244"},"PeriodicalIF":2.9,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144890263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Embryonic temperature shapes morphology, physiology, and survival across life stages for an ectotherm threatened by global change","authors":"Julia M. Thulander ,&nbsp;Joshua M. Hall","doi":"10.1016/j.jtherbio.2025.104215","DOIUrl":"10.1016/j.jtherbio.2025.104215","url":null,"abstract":"<div><div>Climate change and urbanization expose organisms to novel thermal regimes. These changes are particularly impactful for embryonic development of ectotherms due to a lack of parental care, thermal sensitivity of development, and inability to behaviorally thermoregulate. Although the effects of shifting thermal regimes have been studied extensively using a few vertebrate models, little is known about impacts on species with multiple, distinct life stages such as amphibians. We measured nest temperatures of the Streamside Salamander (<em>Ambystoma barbouri</em>) in both disturbed and undisturbed habitats to determine how urbanization affects embryonic temperature. We incubated eggs across a range of temperatures to understand the effects of temperature on survival and larval and juvenile phenotypes. Resultant larvae and metamorphs were maintained in a common garden to isolate the effects of embryonic temperature, illuminating the effect of nest temperature on each life stage (egg, larvae, juvenile). Maximum nest temperatures were warmer in disturbed than undisturbed sites. Warmer incubation temperatures enhanced developmental rate but decreased survival and body size. These effects persisted into larval and metamorph stages, demonstrating that the effects of embryonic temperature persist across life stages. Our results illustrate the need for a deeper understanding of the long-term effects of nest temperature on ectotherms. Moreover, this work indicates that studies which use egg survival and hatchling phenotypes alone to forecast species responses to global change are conservative, and future work must incorporate the full life cycle.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"132 ","pages":"Article 104215"},"PeriodicalIF":2.9,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144860857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heat stroke-induced central nervous system injury: Mechanisms and therapeutic perspectives","authors":"Wenjun Yang ,&nbsp;Zhen Gao ,&nbsp;Chengjin Wang ,&nbsp;Min Wang ,&nbsp;Yuan Cao ,&nbsp;Yun Li ,&nbsp;Shaoqing Shi ,&nbsp;Yiqi Wu ,&nbsp;Lu Wang ,&nbsp;Hongjun Kang","doi":"10.1016/j.jtherbio.2025.104263","DOIUrl":"10.1016/j.jtherbio.2025.104263","url":null,"abstract":"<div><div>Heat stroke (HS), a life-threatening heat-related disorder, is characterized by a rapid elevation of core body temperature exceeding 40 °C, accompanied by central nervous system (CNS) dysfunction and multiple organ dysfunction syndrome (MODS). With the escalating impact of global warming, the incidence of HS has risen progressively, posing a significant threat to global health. The CNS is one of the primary target organs in HS, and its injury mechanisms involve intricate interactions among inflammatory cascades, oxidative stress, programmed cell death, and blood-brain barrier (BBB) disruption. These pathological processes synergistically contribute to neuronal damage, functional impairment, potential long-term cognitive and motor deficits. This review synthesizes current evidence on the molecular and cellular mechanisms underlying HS-induced CNS injury, explores the current progress in therapeutic strategies, and proposes future research directions to address critical knowledge gaps.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"132 ","pages":"Article 104263"},"PeriodicalIF":2.9,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145010342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Thermal stability of pigment- and structurally based body coloration in a polymorphic lizard","authors":"Cristina Romero-Diaz ,&nbsp;Enrique Font ,&nbsp;Guillem Pérez i de Lanuza","doi":"10.1016/j.jtherbio.2025.104262","DOIUrl":"10.1016/j.jtherbio.2025.104262","url":null,"abstract":"<div><div>Animal coloration plays a fundamental role in communication, camouflage, aposematism, mimicry and thermoregulation, and has strong implications for adaptation and diversification. Phenotypic plasticity of color traits can thus affect social, reproductive, antipredator, or thermoregulatory behavior and determining the causes and consequences of color change helps us understand evolution. In contrast to seasonal or ontogenetic color changes, physiological color change in response to fine-scale changes in environmental conditions has received less attention. Temperature-driven, rapid changes in coloration can have profound implications for ecophysiology, particularly in ectotherms. Here, using a widespread color polymorphic lacertid, <em>Podarcis muralis</em>, we study the impact of temperature on the chromatic properties and perception of pigment- and structurally based coloration of different body regions. We subjected male and female adult lizards from different color morphs to two different temperature regimes: cold (25 °C) and hot (≥34 °C) temperature, and quantified color change in the dorsum, belly, throat and ultraviolet (UV)-blue ventrolateral patches using a spectrophotometer. We then applied visual modeling to assess color variation from the perspective of a conspecific and two visual predators. We show that despite minor differences in spectral reflectance metrics, temperature had no significant effect on how color patches are perceived by receivers. Moreover, temperature did not affect existing sex and morph differences in color properties, suggesting that the minor changes we observed have little functional/adaptive consequences in this context. Contrary to results reported in other lizard taxa (iguanians and geckos), our findings suggest that temperature-induced rapid visual color change is unlikely in this lacertid species.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"132 ","pages":"Article 104262"},"PeriodicalIF":2.9,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145003678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Higher temperatures shorten inter-nesting periods in olive ridley turtles","authors":"Laura Fuentes-Tejada ,&nbsp;Pilar Santidrián Tomillo ,&nbsp;Chelsea E. Durr ,&nbsp;Daniel Oliveira Cutrim ,&nbsp;Verónica Valverde-Cantillo ,&nbsp;Frank V. Paladino ,&nbsp;Nathan J. Robinson","doi":"10.1016/j.jtherbio.2025.104249","DOIUrl":"10.1016/j.jtherbio.2025.104249","url":null,"abstract":"<div><div>Climate change is impacting sea turtles worldwide with the effects varying between species and populations. For example, rising temperatures have variable effects on the duration of the inter-nesting period (IP)—the time between two consecutive nests during a single nesting season. Specifically, a negative correlation between water temperature and IP has been reported in green (<em>Chelonia mydas</em>), hawksbill (<em>Eretmochelys imbricata</em>), and loggerhead (<em>Caretta caretta</em>) turtles. In contrast, previous studies have shown no correlation for olive ridley turtles (<em>Lepidochelys olivacea</em>). Here, we assessed whether this lack of a correlation in olive ridley turtles was a product of the small sample size used in previous studies (n_max = 11). We used capture-mark-recapture data over 14 years to calculate the IP of olive ridley turtles (n = 96) nesting on two different beaches in Costa Rica. Next, we calculated mean sea surface temperature (SST) during each IP within estimated inter-nesting areas (225 km²), as inferred from previous olive ridley telemetry studies, and using data from NASA's multi-scale ultra-high-resolution sensor. Mean (±SD) IP was 22.5 ± 6.0 d (range: 13–41 d) and mean SST was 28.0 ± 0.6 °C (range: 26.1–29.5 °C). We observed a statistically significant negative relationship between SST and IP suggesting that elevated temperatures at inter-nesting habitats influence the phenology of olive ridley turtles as previously observed in other hard-shelled sea turtle species. Thus, we postulate that elevated temperatures due to climate change may influence the timing of nesting seasons for solitary nesting olive ridley turtles and may even alter timing of the olive ridley mass nesting aggregations known as <em>arribadas</em>.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"132 ","pages":"Article 104249"},"PeriodicalIF":2.9,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144892160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Predicting potential climate-driven changes in activity time for the critically endangered Kaputar rock skink (Egernia roomi)","authors":"Daniel T. Roberts,&nbsp;Martín Iglesias,&nbsp;Jules E. Farquhar ,&nbsp;David G. Chapple","doi":"10.1016/j.jtherbio.2025.104242","DOIUrl":"10.1016/j.jtherbio.2025.104242","url":null,"abstract":"<div><div>High-elevation ectotherms are vulnerable to climate change due to their restricted ranges, limited dispersal capacity, and reliance on external temperatures for physiological regulation. In Australia, montane regions are limited in extent and elevation, making their cold-adapted fauna especially sensitive to warming. <em>Egernia roomi</em> (Kaputar rock skink) is a Critically Endangered, crevice-dwelling lizard endemic to the high-elevation areas of Mount Kaputar, New South Wales, Australia. Despite its elevated conservation status, little is known about its thermal ecology or climate sensitivity. We projected potential impacts of climate change on thermal activity by quantifying the thermal buffering capacity of rock crevices, and modelling changes in activity time under future climate scenarios. Field-deployed operative temperature models were used to compare crevice and exposed microhabitats across the species' activity season. These data were combined with biophysical modelling (NicheMapR) to simulate body temperature, activity time, and water loss under present and projected climatic conditions. Crevices consistently provided strong thermal buffering, remaining up to 28 °C cooler than exposed surfaces during the day. NicheMapR model predictions aligned with observed temperatures, and predicted substantial increases in daily thermal activity time under moderate to high emissions scenarios (up to +1.6 h/day by 2090 under RCP8.5). These findings offer insight into the species’ thermoregulatory potential and highlight the importance of fine-scale microhabitat features in shaping climate vulnerability.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"132 ","pages":"Article 104242"},"PeriodicalIF":2.9,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144893251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}