Intermittent mild cold stimulation ameliorates hypothermic damage to broiler pectoral muscle by modulating the Nrf2/Keap1/AREs and AMPK/PGC-1α signaling pathways

IF 2.9 2区生物学 Q2 BIOLOGY

Journal of thermal biology Pub Date : 2025-09-05 DOI:10.1016/j.jtherbio.2025.104254

Yuanyuan Liu , Qiang Xue , Hongyu Wang , Yong Zhang , Shijie Zhang , Haidong Wei , Lu Xing , Jianhong Li

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引用次数: 0

Abstract

To investigate the potential protective effect of intermittent cold stimulation in pectoral muscle of broilers. Firstly, we had divided broilers into control (CC) and cold exposure (C3 and C9) groups. Myoblasts were divided into control (C37) and cold exposure (C32 and C27) groups. The results showed that the value of L∗ (lightness) and a∗ (redness) in C9 group were lower than CC and C3 groups, whereas the value of b∗ (yellowness) and drip loss (Myofibre leakage and loss of water, iron and protein during the transition from muscle to meat) was higher than C3 group. The results showed that the mRNA and protein levels of HSPs were significantly higher in the C9 group compared with the CC group. Additionally, apoptosis-associated protein levels of Bcl-2/Bax, CytC, Caspase3, Caspase9 and Caspase8 were significantly elevated in the cold stimulation group, but the levels of Bcl-2/Bax were significantly reduced in the C9 group. Meanwhile, cold-stimulation increased CAT levels and activated the Nrf2 signaling pathway in the C3 group, but not in the C9 group. Moreover, AMPK and PGC-1α were significantly increased after cold stimulation. In vitro results showed that the protein levels of HSP60 and HSP70 were significantly reduced in the C32 and C27 groups compared with the C37 group. Moreover, apoptosis-related protein levels of Bcl-2/Bax, CytC, Caspase3, Caspase9 and Caspase8 were significantly increased in the cold-stimulated group. Cold stimulation increased the mRNA levels of GPx and decreased the mRNA levels of CAT and SOD2 in the C32 group. Meanwhile, the Nrf2 signaling pathway was activated in the cold-stimulated group. Additionally, AMPK and PGC-1α were significantly increased in the C32 group and significantly decreased in the C27 group compared with the C37 group. Therefore, intermittent mild cold-stimulation improved antioxidative function and energy metabolism by activated Nrf2/Keap1/AREs and AMPK/PGC-1α signaling pathway in broiler pectoral muscle.

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间歇性轻度冷刺激通过调节Nrf2/Keap1/AREs和AMPK/PGC-1α信号通路改善肉仔鸡胸肌低温损伤

探讨间歇性冷刺激对肉鸡胸肌的潜在保护作用。首先，将肉鸡分为对照组（CC）和冷暴露组（C3和C9）。将成肌细胞分为对照组（C37）和冷暴露组（C32和C27）。结果表明，C9组肉鸡的L *（亮度）和a *（红度）值低于CC和C3组，而b *（黄度）和滴水损失（肌转肉过程中肌纤维渗漏和水分、铁和蛋白质的损失）值高于C3组。结果表明，与CC组相比，C9组HSPs mRNA和蛋白水平显著升高。此外，冷刺激组细胞凋亡相关蛋白Bcl-2/Bax、CytC、Caspase3、Caspase9和Caspase8水平显著升高，而C9组细胞凋亡相关蛋白Bcl-2/Bax水平显著降低。同时，冷刺激增加了C3组的CAT水平，激活了Nrf2信号通路，而C9组则没有。冷刺激后AMPK和PGC-1α显著升高。体外实验结果显示，C32、C27组与C37组相比，HSP60、HSP70蛋白水平明显降低。冷刺激组细胞凋亡相关蛋白Bcl-2/Bax、CytC、Caspase3、Caspase9、Caspase8水平显著升高。冷刺激使C32组GPx mRNA水平升高，CAT和SOD2 mRNA水平降低。同时，冷刺激组Nrf2信号通路被激活。AMPK和PGC-1α在C32组较C37组显著升高，在C27组较C37组显著降低。因此，间歇性轻度冷刺激可通过激活Nrf2/Keap1/AREs和AMPK/PGC-1α信号通路改善肉仔鸡胸肌的抗氧化功能和能量代谢。

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来源期刊

Journal of thermal biology 生物-动物学

CiteScore

5.30

自引率

7.40%

发文量

196

审稿时长

14.5 weeks

期刊介绍： The Journal of Thermal Biology publishes articles that advance our knowledge on the ways and mechanisms through which temperature affects man and animals. This includes studies of their responses to these effects and on the ecological consequences. Directly relevant to this theme are: • The mechanisms of thermal limitation, heat and cold injury, and the resistance of organisms to extremes of temperature • The mechanisms involved in acclimation, acclimatization and evolutionary adaptation to temperature • Mechanisms underlying the patterns of hibernation, torpor, dormancy, aestivation and diapause • Effects of temperature on reproduction and development, growth, ageing and life-span • Studies on modelling heat transfer between organisms and their environment • The contributions of temperature to effects of climate change on animal species and man • Studies of conservation biology and physiology related to temperature • Behavioural and physiological regulation of body temperature including its pathophysiology and fever • Medical applications of hypo- and hyperthermia Article types: • Original articles • Review articles