Promising role of dietary microalgae blend in alleviating the heat stress draw backs in broiler chickens: Impact on performance, meat antioxidants and fatty acids content and expression of heat shock protein and sirtuins related genes

IF 2.9 2区 生物学 Q2 BIOLOGY
Doaa Ibrahim , Mohamed Shawky , Asmaa EL- Sayed Kamel , Ahmed Abdelfattah-Hassan , Rahma Taha , Samah S. Khalil , Mohamed Tharwat Elabbasy , Asmaa T.Y. Kishawy
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引用次数: 0

Abstract

Adopting innovative nutritional strategies, such as microalgae blend supplementation, can help reduce the detrimental effects of heat stress (HS) in poultry farming. This study was designed to explore the effects of different dietary levels of microalgae blend containing nannochloropsis oculate, schizochytrium and amphora coffesformis on modulating growth performance, muscle's antioxidant status, lipid profile and the expression of heat shock proteins and sirtuins family related genes in heat-stressed chicks. A total of 250 one-day-old chicks were randomly assigned to five experimental groups, with five replicates of 10 birds each: a control group reared under normal environmental conditions and fed a basal diet; HS group exposed to heat stress and fed a basal diet; and three HS groups supplemented with a microalgae blend at 2.5, 5, and 10 g/kg of diet, respectively. A notable improvement in total growth and FCR especially was recorded in group exposed to HS and supplemented with 10 g/kg diet of microalgae blend compared to HS group. Moreover, the group exposed to HS and fortified with microalgae blend at the concentration of 10 g/kg diet showed reduced cholesterol and triacylglycerol levels, comparable to those of the control group. Notably, all heat-stressed groups supplemented with the microalgae blend showed no significant changes in blood parameters compared to the control group. In the heat-stressed group, dietary supplementation with 10 g/kg of the microalgae blend significantly reduced the levels of ALT, uric acid, AST, creatinine, corticosterone, and thyroid hormones (T3 and T4), bringing them closer to normal physiological ranges. With increasing levels of the microalgae blend, there was a marked enhancement in the activity of muscle antioxidant enzymes, including glutathione peroxidase (GSH-Px), catalase, and superoxide dismutase (SOD), along with an increase in T-AOC content, accompanied by a significant reduction in MDA levels. Notably, increasing the concentration of the microalgae blend resulted in elevated levels of n-3 PUFAs, particularly eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), in the breast muscle. Relative mRNA expression levels of Hsp70, Hsp90, SIRT1, SIRT3, and SIRT7 were elevated in the heat-stressed groups; however, the group supplemented with 10 g/kg of the microalgae blend exhibited the most pronounced downregulation of these genes. In conclusion, this study underscores the potential of the microalgae blend to mitigate the adverse effects of heat stress in poultry farming via modulating antioxidant activity, upregulating expression of sirtuins family and attenuating the overexpression of heat shock proteins.
饲粮微藻混合物在缓解肉鸡热应激缩回中的重要作用:对肉鸡生产性能、肉类抗氧化剂和脂肪酸含量以及热休克蛋白和sirtuins相关基因表达的影响
采用创新的营养策略,如添加微藻混合物,有助于减少家禽养殖中热应激(HS)的有害影响。本试验旨在探讨饲粮中添加不同水平的含尾状纳米绿藻、裂藻和咖啡双耳藻的微藻混合物对热应激雏鸡生长性能、肌肉抗氧化状态、脂质特征以及热休克蛋白和sirtuins家族相关基因表达的影响。选取250只1日龄雏鸡,随机分为5个试验组,每组5个重复,每组10只:对照组在正常环境下饲养,饲喂基础饲粮;HS组热应激,饲喂基础日粮;3个HS组分别添加2.5、5和10 g/kg微藻混合物。与HS组相比,暴露于HS并添加10 g/kg微藻混合物组的总生长和FCR显著提高。此外,以10 g/kg的浓度暴露于HS和添加微藻混合物的组的胆固醇和甘油三酯水平降低,与对照组相当。值得注意的是,与对照组相比,所有添加微藻混合物的热应激组的血液参数均无显著变化。在热应激组,饲粮中添加10 g/kg微藻混合物可显著降低ALT、尿酸、AST、肌酐、皮质酮和甲状腺激素(T3和T4)水平,使其接近正常生理范围。随着微藻混合物水平的增加,肌肉抗氧化酶活性显著增强,包括谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶和超氧化物歧化酶(SOD), T-AOC含量增加,MDA水平显著降低。值得注意的是,增加微藻混合物的浓度导致胸肌中n-3 PUFAs水平升高,特别是二十碳五烯酸(EPA)和二十二碳六烯酸(DHA)。热应激组Hsp70、Hsp90、SIRT1、SIRT3、SIRT7 mRNA相对表达量升高;然而,添加10 g/kg微藻混合物的组表现出最明显的这些基因下调。总之,本研究强调了微藻混合物通过调节抗氧化活性、上调sirtuins家族表达和减弱热休克蛋白的过度表达来减轻家禽养殖中热应激的不利影响的潜力。
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来源期刊
Journal of thermal biology
Journal of thermal biology 生物-动物学
CiteScore
5.30
自引率
7.40%
发文量
196
审稿时长
14.5 weeks
期刊介绍: The Journal of Thermal Biology publishes articles that advance our knowledge on the ways and mechanisms through which temperature affects man and animals. This includes studies of their responses to these effects and on the ecological consequences. Directly relevant to this theme are: • The mechanisms of thermal limitation, heat and cold injury, and the resistance of organisms to extremes of temperature • The mechanisms involved in acclimation, acclimatization and evolutionary adaptation to temperature • Mechanisms underlying the patterns of hibernation, torpor, dormancy, aestivation and diapause • Effects of temperature on reproduction and development, growth, ageing and life-span • Studies on modelling heat transfer between organisms and their environment • The contributions of temperature to effects of climate change on animal species and man • Studies of conservation biology and physiology related to temperature • Behavioural and physiological regulation of body temperature including its pathophysiology and fever • Medical applications of hypo- and hyperthermia Article types: • Original articles • Review articles
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