Journal of microencapsulation最新文献

{"title":"siRNA-guided dual-targeting nanocarrier for breast cancer treatment.","authors":"Ebru Kilicay, Betul Sahin, Zeynep Karahaliloglu, Ekin Celik, Baki Hazer","doi":"10.1080/02652048.2025.2490041","DOIUrl":"10.1080/02652048.2025.2490041","url":null,"abstract":"<p><strong>Aims: </strong>This study aimed to develop a thermoplastic polyurethane-oleic acid-based nanosystem (TPU-Ole NPs) incorporating siRNA and curcumin (CUR) to overcome multidrug resistance in breast cancer by silencing the c-myc gene.</p><p><strong>Methods: </strong>TPU-Ole and CUR-loaded NPs were prepared via solvent evaporation and coated with poly-L-lysine (PLL) for siRNA attachment. NPs were characterised by dynamic light scattering (DLS) for mean diameter, polydispersity index (PDI), and zeta potential (ZP). Encapsulation (EE) and loading efficiencies (LE) were measured by NanoDrop. Release (pH 5.0; 7.4) and storage stability (pH 7.4) were evaluated using the eppendorf method. siRNA binding was confirmed by agarose gel electrophoresis. Gene silencing and apoptosis were assessed by RT-PCR and flow cytometry.</p><p><strong>Results: </strong>Mean diameter, PDI, and ZP of NPs were 170 ± 2 nm, 0.011 ± 0.080, and -27.5 ± 0.11 mV. EE and LE were 75 ± 0.12 and 14.2 ± 0.06%. Sustained release and good stability were observed.</p><p><strong>Conclusion: </strong>siRNA-CUR-NPs efficiently silenced c-myc and induced apoptosis in MCF-7 cells.</p>","PeriodicalId":16391,"journal":{"name":"Journal of microencapsulation","volume":" ","pages":"437-458"},"PeriodicalIF":3.2,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144022789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling the synergistic impact of cocktail nanohyalurosomes encapsulating berberine and dexamethasone in managing rheumatoid arthritis: in-vitro evaluation and biological studies.","authors":"Mariam Zewail, Manal A Elsheikh, Haidy Abbas, Passent M E Gaafar","doi":"10.1080/02652048.2025.2498963","DOIUrl":"10.1080/02652048.2025.2498963","url":null,"abstract":"<p><strong>Aim: </strong>The current therapeutic approaches for rheumatoid arthritis (RA) have limited effectiveness; the present study focused on the formulation of hyalurosomes co-encapsulating dexamethasone and berberine (BER-DEX hyalurosomes).</p><p><strong>Methods: </strong>Different formulations were developed and in-vitro characterized. The optimized formulation was transdermally applied in rats with AIA model. At the end of the experiment, histopathological examination and evaluation of inflammatory biomarkers were conducted.</p><p><strong>Results: </strong>Entrapment efficiency of 81.14 ± 2.36% for DEX and 92.69 ± 1.58% for BER was achieved with a sustained release for 24h for both drugs. TNF-α, IL7, MMP9, and HO levels decreased by 2.7, 2.4, 2.24, and 3.6 folds in BER-DEX hyalurosomes compared to the positive control group. Histopathological assessment revealed that BER-DEX hyalurosomes showed normal joint structure comparable to the negative control.</p><p><strong>Conclusion: </strong>The BER-DEX hyalurosomes offered a synergistic, non-invasive nanoplatform that actively targeted CD44 receptors, provided a novel and effective strategy for localized management of RA..</p>","PeriodicalId":16391,"journal":{"name":"Journal of microencapsulation","volume":" ","pages":"503-518"},"PeriodicalIF":3.2,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144022644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advanced chitosan-based biopolymer systems for probiotic microencapsulation: stability enhancement and targeted release approaches.","authors":"Great Iruoghene Edo, Alice Njolke Mafe, Ali B M Ali, Emad Yousif, Raghda S Makia, Endurance Fegor Isoje, Ufuoma Augustina Igbuku, Patrick Othuke Akpoghelie, Joseph Oghenewogaga Owheruo, Rapheal Ajiri Opiti, Arthur Efeoghene Athan Essaghah, Dina S Ahmed, Huzaifa Umar","doi":"10.1080/02652048.2025.2531776","DOIUrl":"https://doi.org/10.1080/02652048.2025.2531776","url":null,"abstract":"<p><strong>Context: </strong>Probiotic microencapsulation is an advanced technique designed to protect live probiotic microorganisms by enclosing them within a protective matrix, typically composed of biocompatible biopolymers.</p><p><strong>Objective: </strong>This review provides a comprehensive analysis of recent advancements in bio-polymer coatings for probiotic microencapsulation, with a focus on chitosan and its synergistic combinations with other materials.</p><p><strong>Methods: </strong>This methods highlights the necessity for continued innovation in bio-polymer coatings, emphasizing the development of bio-responsive materials, AI-driven formulation strategies, and next-generation encapsulation technologies to meet the evolving demands of functional foods and precision therapeutics.</p><p><strong>Results: </strong>Probiotic microencapsulation plays a critical role in protecting probiotics from environmental stresses, improving stability, and ensuring targeted delivery. Innovations in chitosan-based encapsulation, including its combination with bio-polymers such as alginate, gelatin, and pectin, have enhanced encapsulation efficiency, controlled release, and probiotic viability. Cutting-edge techniques such as nanotechnology, stimuli-responsive coatings, and hybrid bio-polymers are explored for their potential to optimize probiotic performance in food and pharmaceutical applications.</p><p><strong>Conclusions: </strong>Despite these advancements, obstacles remain in ensuring consistent release profiles, mitigating the inhibitory effects of chitosan on certain probiotic strains, and optimizing large-scale production while maintaining cost-effectiveness. The need for personalized probiotic therapies has driven research into adaptive encapsulation systems tailored to individual gut microbiota compositions.</p>","PeriodicalId":16391,"journal":{"name":"Journal of microencapsulation","volume":" ","pages":"1-25"},"PeriodicalIF":3.0,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144690546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanoemulsions for topical delivery: formulation, applications and recent advances.","authors":"Rajvi Shah, Jimil Gandhi, Monit Shah, Prerna Tiwari, Bharat Chaudhary, Vidhi Shah","doi":"10.1080/02652048.2025.2531778","DOIUrl":"https://doi.org/10.1080/02652048.2025.2531778","url":null,"abstract":"<p><p>Nanoemulsion (NE) is a kinetically stable dispersion comprising oil and water stabilised by an emulsifier, having a droplet size of around 20-200 nm. NE offers versatile formulations for lipophilic and hydrophilic drugs by increasing their solubility and permeability thereby improving their bioavailability. Various high energy and low energy techniques such as the phase pH inversion method, solvent diffusion method, and membrane emulsification method are reported to develop NE. The selection of method is crucial, thus a comparison of different methods in terms of droplet size and stability has been provided. This review emphasises the topical application of NE for various skin ailments like psoriasis, skin infection, cancer, wound healing, and many others. This review highlights the advancement in the NE formulation using ionic liquids and nanoparticles, as well as stimuli-responsive and pickering NE. The approaches to overcome the toxicity concern of NE and its constituents specifically surfactant has been discussed.</p>","PeriodicalId":16391,"journal":{"name":"Journal of microencapsulation","volume":" ","pages":"1-22"},"PeriodicalIF":3.0,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144690547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PLGA-lecithin nanocarrier encapsulating curcuma caesia oil in a mucoadhesive gel: efficacy analysis against periodontal infections.","authors":"Bhabani Sankar Satapathy, Mahaprasad Mohanty, Sritam Behera","doi":"10.1080/02652048.2025.2531784","DOIUrl":"https://doi.org/10.1080/02652048.2025.2531784","url":null,"abstract":"<p><strong>Aim: </strong>The study reported development of <i>Curcuma caesia</i> leaf essential oil encapsulated poly(lactic-co-glycolic acid) (PLGA) nanocarrier-embedded gel as a potential strategy to treat periodontal infection.</p><p><strong>Method: </strong>\u0000 <i>Curcuma caesia oil loaded experimental nanocarriers (CNCs) were developed by nano precipitation method and characterized. In vitro and in vivo efficacy of CNCs loaded gel (CNCsG) was evaluated.</i>\u0000 </p><p><strong>Results: </strong>GC/MS analysis revealed Borneol as the major phytoactive constituent. CNCs were spherical, nanosized (51.28 ± 2.1 nm), with -40.5 ± 0.8% mV surface charge, 20.8 ± 0.7% w/w oil loading and 86.4 ± 1.3% encapsulation efficiency. CNCsG was reported with satisfied mucoadhesion (45.66 ± 2.2 dyne/cm2), viscosity (37745 ± 32.7 cps), spreadability (6.5 ± 1.2 gm.cm/sec), sustained ex-vivo release. A faster recovery of infection towards normal was observed in periodontitis rats by CNCsG than Clorni gel. Improved plasma pharmacokinetic profile was reported for CNCsG as compared to standard gel.</p><p><strong>Conclusion: </strong>CNCsG was reported having promising antibacterial potency. <i>In vivo</i> efficacy data would lay foundation for futuristic clinical testing of CNCsG in periodontitis.</p>","PeriodicalId":16391,"journal":{"name":"Journal of microencapsulation","volume":" ","pages":"1-19"},"PeriodicalIF":3.0,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144649628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic anti-breast cancer activity of simvastatin and thymoquinone encapsulated in nanostructured lipid carriers for enhanced therapeutic outcomes.","authors":"Pallavi Kumari, Javed Ali, Sanjula Baboota, Amit Tyagi, Shweta Dang","doi":"10.1080/02652048.2025.2521077","DOIUrl":"https://doi.org/10.1080/02652048.2025.2521077","url":null,"abstract":"<p><p>This study aims to study the combined anti-cancer effects of Simvastatin (SIM) and Thymoquinone (THY) against breast cancer cell lines and to develop and evaluate nanostructured lipid carriers (NLCs) encapsulating Simvastatin and Thymoquinone. Nanostructured lipid carriers (NLCs) co-loaded with Simvastatin and Thymoquinone were successfully formulated for enhanced anticancer activity. The formulations were characterised using Transmission Electron Microscopy (TEM), Scanning Electron Microscopy (SEM), and X-Ray Diffraction (XRD) to determine particle morphology and crystallinity. Key physicochemical parameters, including mean particle size, polydispersity index (PDI), zeta potential, and drug loading capacity, were systematically evaluated. Encapsulation efficiency and in vitro drug release profiles were assessed using the dialysis bag diffusion method, while formulation stability was monitored at 4 °C, 25 °C, and 40 °C over a defined period. In vitro biological evaluations were conducted on MCF-7 and MDA-MB-231 breast cancer cell lines. Cytotoxic potential was determined through MTT assay and IC50 value estimation. Cellular uptake was visualised using fluorescence microscopy, and apoptosis induction was quantified via flow cytometry using Annexin V-FITC/PI dual staining. The results suggest that the co-delivery of Simvastatin and Thymoquinone via NLCs enhances intracellular drug accumulation and promotes apoptosis, highlighting their potential as a synergistic nanocarrier-based therapy for breast cancer treatment. The optimised SIM-THY-NLCs were spherical with mean diameter of 105.6 ± 4.2 nm, PDI of 0.214 ± 0.03, and zeta potential of -28.6 ± 2.1 mV. Encapsulation efficiencies were 89 ± 1.59% (SIM) and 91 ± 1.45% (THY). Sustained drug release over 24 h was observed (SIM: 40%, THY: 65%). The NLCs showed significantly improved cytotoxicity with IC₅₀ values of 2.56 µg/ml (MCF-7) and 1.23 µg/ml (MDA-MB-231), alongside enhanced cellular uptake and apoptosis. SIM-THY-NLCs significantly improve drug stability, release, and anticancer efficacy on breast cancer cells establishing them as a promising nanocarrier system for effective breast cancer therapy.</p>","PeriodicalId":16391,"journal":{"name":"Journal of microencapsulation","volume":" ","pages":"1-20"},"PeriodicalIF":3.0,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel combinational nanomedicines, liposomes, to tackle breast cancer.","authors":"Mohamed Attia, David Hill, Cheng Shu Chaw, Amal Ali Elkordy","doi":"10.1080/02652048.2025.2487031","DOIUrl":"10.1080/02652048.2025.2487031","url":null,"abstract":"<p><strong>Aims: </strong>Doxorubicin (DOX), a potent chemotherapeutic, is a commonly prescribed treatment for breast cancer, but is limited by severe organ toxicity. Therefore, more effective therapies are required. This study developed a novel DOX-liposomes (LipDOX-ALA-AA) co-loaded with alpha-lipoic-acid (ALA) and ascorbic-acid (AA) to enhance antineoplastic effect.</p><p><strong>Methods: </strong>Liposomes were fabricated using a microfluidic-system with a DSPClipid:Cholesterol ratio of 1:1 and a flow rate ratio of 5:1. Liposomes were investigated using various-techniques such-as dynamic light scattering to measure liposomes' size and charge; and UV-spectroscopy to determine DOX-encapsulation-efficiency, EE. Cytotoxicity assays used various cell-lines.</p><p><strong>Results: </strong>Data revealed that LipDOX-ALA-AA had diameter of 79.0 ± 0.3 nm, with narrow size distribution, and zeta-potential of -4.0 ± 1.2. DOX-EE exceeded 95%, drug load was 0.5 mg/105.5 mg total content, drug release followed a biphasic pattern. Cytotoxicity assay showed activity (<i>p</i> < 0.05) against breast cancer cell-lines with reduced nephrotoxicity compared to Doxosome.</p><p><strong>Conclusion: </strong>This novel formulation (LipDOX-ALA-AA) offers a promise in breast cancer therapy.</p>","PeriodicalId":16391,"journal":{"name":"Journal of microencapsulation","volume":" ","pages":"368-391"},"PeriodicalIF":3.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143788353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intra-articular treatment of osteoarthritis using novel biocompatible etoricoxib chitosan-hyaluronate hybrid microparticles.","authors":"Khaled E Abuelella, Hend Abd-Allah, Sara M Soliman, Mona M A Abdel-Mottaleb","doi":"10.1080/02652048.2025.2490033","DOIUrl":"10.1080/02652048.2025.2490033","url":null,"abstract":"<p><strong>Aim: </strong>The current study aimed to develop and evaluate Etoricoxib (ETX) loaded polyelectrolyte microparticles (PEMPs) for intra-articular delivery in osteoarthritis management.</p><p><strong>Methods: </strong>PEMPs were prepared by the electrostatic interactions between hyaluronic acid (HA) and chitosan (CS). The optimum formulation was characterized for encapsulation efficiency, particle size (PS), zeta potential (ZP), drug release, stability, TEM, FTIR, DSC and in vivo anti-inflammatory activity.</p><p><strong>Results: </strong>The optimum formulation (ME4/TPP0.25) demonstrated spherical particles with a PS of 1.56 ± 0.04 µm, a PDI value of 0.29 ± 0.05, ZP of +35.26 ± 0.9 mV, and EE% of 94.7 ± 0.24% and loading capacity of 11.7 ± 0.16% (w/w). In vivo studies demonstrated that ME4/TPP0.25 significantly suppressed knee joint swelling, and significantly reduced the levels of catabolic and inflammatory mediators (ALP and IL-6) compared to drug alone.</p><p><strong>Conclusion: </strong>These results suggest that the optimum ETX-loaded PEMPs could be a promising formulation for knee osteoarthritis management.</p>","PeriodicalId":16391,"journal":{"name":"Journal of microencapsulation","volume":" ","pages":"421-435"},"PeriodicalIF":3.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144007151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"β-Cyclodextrin-functionalized nanocarriers for bromocriptine: development, evaluation and histopathological studies.","authors":"Muhammad Ahsan Waqar, Iqra Noor Khan, Shabab Zahra, Farwa Shaheen, Saba Noureen, Shakeel Ahmad, Muhammad Irfan Siddique, Muhammad Nadeem Alvi","doi":"10.1080/02652048.2025.2487034","DOIUrl":"10.1080/02652048.2025.2487034","url":null,"abstract":"<p><p>Bromocriptine (BCM), a dopaminergic agonist used in Parkinson's disease treatment, has poor oral bioavailability due to extensive first-pass metabolism and limited gastrointestinal absorption. This study aimed to develop a β-cyclodextrin-functionalized bromocriptine nanoemulsion (oil-in-water) to enhance drug solubility, stability, and bioavailability while facilitating direct brain delivery via the intranasal route. The formulation was designed to overcome systemic metabolic barriers, improve drug permeation across the blood-brain barrier, and ensure sustained therapeutic effects with minimal systemic side effects. Nano-emulsions were prepared using high-shear homogenization. Characterization was performed using scanning electron microscopy (SEM) for morphological analysis. Globule size and zeta potential were measured using Malvern Zetasizer. Fourier Transform Infrared Spectroscopy (FTIR) was used for structural analysis, while X-ray diffraction (XRD) assessed crystallinity. Differential Scanning Calorimetry (DSC) was conducted for thermal analysis. Drug content and <i>in-vitro</i> drug release were evaluated using UV-visible spectroscopy. Stability studies were performed using centrifugation and freeze-thaw methods. Docking studies and Histopathological evaluation were also performed of the prepared formulations. Morphological studies revealed nano-sized globular particles with a mean diameter of 117.2 nm and a low polydispersity index (PDI 0.810), indicating uniformity. The nanoemulsion exhibited a zeta potential of -10.5 mV, ensuring colloidal stability. The encapsulation efficiency (EE%) of the optimized formulation (F4) was 95.36(% w/w,) with a drug load of approximately 9.5(% w/w). <i>In-vitro</i> drug release reached 85.65%, with permeation release of 78.44% and 70.13% ex-vivo. The formulation remained stable under freeze-thaw and centrifugation conditions. Cell toxicity assessments demonstrated excellent biocompatibility, with no significant cytotoxic effects observed in histopathological evaluations. This nanoemulsion presents a promising alternative to oral bromocriptine for Parkinson's treatment.</p>","PeriodicalId":16391,"journal":{"name":"Journal of microencapsulation","volume":" ","pages":"406-420"},"PeriodicalIF":3.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143988501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cellulose-based hydrogels enhanced with bioactive molecules for optimal chronic diabetic wound management.","authors":"Mahtab Ghasemi Toudeshkchouei, Hassan Abdoos, Jafar Ai, M S Nourbakhsh","doi":"10.1080/02652048.2025.2480598","DOIUrl":"10.1080/02652048.2025.2480598","url":null,"abstract":"<p><p>Hydrogels are three-dimensional structures that replicate natural tissues' extracellular matrix (ECM). They are essential for transporting exudates, gases, and moisture and facilitating cellular interactions in tissue engineering and wound healing. The choice of primary material in designing the scaffold is necessary to be paid more attention rather than common sources, including plant fibres like cotton, bamboo, and algae, as well as bacterial and marine-derived materials. Among them, cellulose-based polymers are especially valued for their biocompatibility and ability to promote wound healing. Chronic diabetic wounds pose unique treatment challenges, such as necrosis and infection risks. Consequently, a growing interest is in incorporating bioactive molecules into cellulose-based hydrogels. This article investigates how these infused hydrogels enhance the healing process in chronic diabetic wounds, examining various loading and crosslinking techniques alongside their clinical applications. It also discusses the benefits and limitations of bioactive molecules and their interactions with hydrogels to improve treatment strategies.</p>","PeriodicalId":16391,"journal":{"name":"Journal of microencapsulation","volume":" ","pages":"313-336"},"PeriodicalIF":3.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143692382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}