Unveiling the synergistic impact of cocktail nanohyalurosomes encapsulating berberine and dexamethasone in managing rheumatoid arthritis: in-vitro evaluation and biological studies.

IF 3 4区医学 Q2 CHEMISTRY, APPLIED

Journal of microencapsulation Pub Date : 2025-05-13 DOI:10.1080/02652048.2025.2498963

Mariam Zewail, Manal A Elsheikh, Haidy Abbas, Passent M E Gaafar

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引用次数: 0

Abstract

Aim: The current therapeutic approaches for rheumatoid arthritis (RA) have limited effectiveness; the present study focused on the formulation of hyalurosomes co-encapsulating dexamethasone and berberine (BER-DEX hyalurosomes).

Methods: Different formulations were developed and in-vitro characterized. The optimized formulation was transdermally applied in rats with AIA model. At the end of the experiment, histopathological examination and evaluation of inflammatory biomarkers were conducted.

Results: Entrapment efficiency of 81.14 ± 2.36% for DEX and 92.69 ± 1.58% for BER was achieved with a sustained release for 24h for both drugs. TNF-α, IL7, MMP9, and HO levels decreased by 2.7, 2.4, 2.24, and 3.6 folds in BER-DEX hyalurosomes compared to the positive control group. Histopathological assessment revealed that BER-DEX hyalurosomes showed normal joint structure comparable to the negative control.

Conclusion: The BER-DEX hyalurosomes offered a synergistic, non-invasive nanoplatform that actively targeted CD44 receptors, provided a novel and effective strategy for localized management of RA..

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揭示包裹小檗碱和地塞米松的鸡尾酒纳米透明质体在类风湿关节炎治疗中的协同作用：体外评估和生物学研究。

目的：目前类风湿性关节炎（RA）的治疗方法有效性有限；本研究的重点是联合包裹地塞米松和小檗碱的透明质体（BER-DEX透明质体）的配方。方法：研制不同配方并进行体外表征。将优化后的配方经皮应用于AIA模型大鼠。实验结束时进行组织病理学检查和炎症生物标志物评价。结果：DEX的包封效率为81.14±2.36%，BER的包封效率为92.69±1.58%，缓释时间为24h。与阳性对照组相比，BER-DEX透明质体中TNF-α、IL7、MMP9和HO水平分别降低了2.7倍、2.4倍、2.24倍和3.6倍。组织病理学评估显示，与阴性对照相比，BER-DEX透明质体显示正常的关节结构。结论：BER-DEX透明质体提供了一种协同的、非侵入性的纳米平台，可主动靶向CD44受体，为局部治疗RA提供了一种新颖有效的策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of microencapsulation 工程技术-工程：化工

CiteScore

6.30

自引率

2.60%

发文量

审稿时长

3 months

期刊介绍： The Journal of Microencapsulation is a well-established, peer-reviewed journal dedicated to the publication of original research findings related to the preparation, properties and uses of individually encapsulated novel small particles, as well as significant improvements to tried-and-tested techniques relevant to micro and nano particles and their use in a wide variety of industrial, engineering, pharmaceutical, biotechnology and research applications. Its scope extends beyond conventional microcapsules to all other small particulate systems such as self assembling structures that involve preparative manipulation. The journal covers: Chemistry of encapsulation materials Physics of release through the capsule wall and/or desorption from carrier Techniques of preparation, content and storage Many uses to which microcapsules are put.