{"title":"载狼疮芽孢杆菌成分的大豆卵磷脂植物体传递系统的制备、表征、抗癌潜力和安全性评价。","authors":"Sabyasachi Banerjee, Shibangi Mukhopadhyay, Avik Das, Subhasis Banerjee, Sankhadip Bose, Santanu Banerjee, Nicolette Casarcia, Anupam Bishayee","doi":"10.1080/02652048.2025.2467046","DOIUrl":null,"url":null,"abstract":"<p><p>In this study, antineoplastic effects of a novel soy lecithin-based phytosome drug delivery system containing <i>Barleria lupulina</i> Lindl. extract (BLSP) was evaluated. BLSP was prepared using the thin-film hydration method and analysed using energy-dispersive X-ray spectroscopy, scanning electron microscopy, X-ray diffraction, and Zetasizer technique. Phytosomes showed a mean-diameter of 135 ± 0.29 nm, zeta potential of -56 ± 1.16 mV, and entrapment efficiency of 57.24 ± 0.12%. The drug release profiles exhibited a two-phase pattern with a protracted and sustained release after the first release. BLSP had a cytotoxic potential against MCF-7 breast and HeLa cervical cancers and demonstrated a concentration-dependent reduction of reactive oxygen species and mitochondrial membrane potential. BLSP caused upregulation of B-cell lymphoma-2-associated-X protein, caspase-8, caspase-9, and cluster of differentiation-95, and downregulation of B-cell lymphoma-2. The <i>in vivo</i> toxicity study showed the safety of BLSP. Overall, BLSP has demonstrated potential as a promising formulation for delivering <i>B. lupulina</i> phytoconstituents to treat breast and cervical cancer.</p>","PeriodicalId":16391,"journal":{"name":"Journal of microencapsulation","volume":" ","pages":"209-229"},"PeriodicalIF":3.2000,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Preparation, characterisation, anticancer potential and safety evaluation of a soy lecithin phytosome delivery system loaded with constituents from <i>Barleria lupulina</i>.\",\"authors\":\"Sabyasachi Banerjee, Shibangi Mukhopadhyay, Avik Das, Subhasis Banerjee, Sankhadip Bose, Santanu Banerjee, Nicolette Casarcia, Anupam Bishayee\",\"doi\":\"10.1080/02652048.2025.2467046\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>In this study, antineoplastic effects of a novel soy lecithin-based phytosome drug delivery system containing <i>Barleria lupulina</i> Lindl. extract (BLSP) was evaluated. BLSP was prepared using the thin-film hydration method and analysed using energy-dispersive X-ray spectroscopy, scanning electron microscopy, X-ray diffraction, and Zetasizer technique. Phytosomes showed a mean-diameter of 135 ± 0.29 nm, zeta potential of -56 ± 1.16 mV, and entrapment efficiency of 57.24 ± 0.12%. The drug release profiles exhibited a two-phase pattern with a protracted and sustained release after the first release. BLSP had a cytotoxic potential against MCF-7 breast and HeLa cervical cancers and demonstrated a concentration-dependent reduction of reactive oxygen species and mitochondrial membrane potential. BLSP caused upregulation of B-cell lymphoma-2-associated-X protein, caspase-8, caspase-9, and cluster of differentiation-95, and downregulation of B-cell lymphoma-2. The <i>in vivo</i> toxicity study showed the safety of BLSP. Overall, BLSP has demonstrated potential as a promising formulation for delivering <i>B. lupulina</i> phytoconstituents to treat breast and cervical cancer.</p>\",\"PeriodicalId\":16391,\"journal\":{\"name\":\"Journal of microencapsulation\",\"volume\":\" \",\"pages\":\"209-229\"},\"PeriodicalIF\":3.2000,\"publicationDate\":\"2025-05-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of microencapsulation\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1080/02652048.2025.2467046\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/2/24 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q2\",\"JCRName\":\"CHEMISTRY, APPLIED\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of microencapsulation","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1080/02652048.2025.2467046","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/2/24 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"CHEMISTRY, APPLIED","Score":null,"Total":0}
引用次数: 0
摘要
在这项研究中，新的大豆卵磷脂为基础的植物体药物传递系统含有狼疮芽孢杆菌的抗肿瘤作用。提取液（BLSP）进行评价。采用薄膜水化法制备了BLSP，并利用能量色散x射线能谱、扫描电镜、x射线衍射和Zetasizer技术对其进行了分析。磷脂质体的平均直径为135±0.29 nm， zeta电位为-56±1.16 mV，包封效率为57.24±0.12%。药物释放表现为两相模式，一次释放后缓释和缓释。BLSP对MCF-7乳腺癌和HeLa宫颈癌具有细胞毒性，并显示出浓度依赖性的活性氧和线粒体膜电位的降低。BLSP引起b细胞淋巴瘤-2相关- x蛋白、caspase-8、caspase-9、cluster of differentiation-95上调，b细胞淋巴瘤-2下调。体内毒性研究表明BLSP是安全的。总的来说，BLSP已被证明有潜力作为一种有前途的配方，提供螺旋藻植物成分治疗乳腺癌和宫颈癌。
Preparation, characterisation, anticancer potential and safety evaluation of a soy lecithin phytosome delivery system loaded with constituents from Barleria lupulina.
In this study, antineoplastic effects of a novel soy lecithin-based phytosome drug delivery system containing Barleria lupulina Lindl. extract (BLSP) was evaluated. BLSP was prepared using the thin-film hydration method and analysed using energy-dispersive X-ray spectroscopy, scanning electron microscopy, X-ray diffraction, and Zetasizer technique. Phytosomes showed a mean-diameter of 135 ± 0.29 nm, zeta potential of -56 ± 1.16 mV, and entrapment efficiency of 57.24 ± 0.12%. The drug release profiles exhibited a two-phase pattern with a protracted and sustained release after the first release. BLSP had a cytotoxic potential against MCF-7 breast and HeLa cervical cancers and demonstrated a concentration-dependent reduction of reactive oxygen species and mitochondrial membrane potential. BLSP caused upregulation of B-cell lymphoma-2-associated-X protein, caspase-8, caspase-9, and cluster of differentiation-95, and downregulation of B-cell lymphoma-2. The in vivo toxicity study showed the safety of BLSP. Overall, BLSP has demonstrated potential as a promising formulation for delivering B. lupulina phytoconstituents to treat breast and cervical cancer.
期刊介绍：
The Journal of Microencapsulation is a well-established, peer-reviewed journal dedicated to the publication of original research findings related to the preparation, properties and uses of individually encapsulated novel small particles, as well as significant improvements to tried-and-tested techniques relevant to micro and nano particles and their use in a wide variety of industrial, engineering, pharmaceutical, biotechnology and research applications. Its scope extends beyond conventional microcapsules to all other small particulate systems such as self assembling structures that involve preparative manipulation.
The journal covers:
Chemistry of encapsulation materials
Physics of release through the capsule wall and/or desorption from carrier
Techniques of preparation, content and storage
Many uses to which microcapsules are put.
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