sirna引导双靶向纳米载体用于乳腺癌治疗。

IF 3 4区医学 Q2 CHEMISTRY, APPLIED

Journal of microencapsulation Pub Date : 2025-05-06 DOI:10.1080/02652048.2025.2490041

Ebru Kilicay, Betul Sahin, Zeynep Karahaliloglu, Ekin Celik, Baki Hazer

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引用次数: 0

摘要

目的：本研究旨在开发一种含有siRNA和姜黄素（CUR）的热塑性聚氨酯油酸纳米系统（TPU-Ole NPs），通过沉默c-myc基因来克服乳腺癌的多药耐药。方法：采用溶剂蒸发法制备TPU-Ole和cu负载的NPs，并包被聚l -赖氨酸（PLL）用于siRNA的附着。用动态光散射（DLS）表征NPs的平均直径、多分散性指数（PDI）和ζ电位（ZP）。采用NanoDrop法测定其包封效率（EE）和负载效率（LE）。发布(pH 5.0；7.4)和储存稳定性（pH 7.4）采用epppendorf法进行评价。琼脂糖凝胶电泳证实了siRNA的结合。采用RT-PCR和流式细胞术检测基因沉默和细胞凋亡。结果：NPs的平均直径为170±2 nm， PDI为0.011±0.080，ZP为-27.5±0.11 mV。EE和LE分别为75±0.12%和14.2±0.06%。观察到缓释和良好的稳定性。结论：siRNA-CUR-NPs能有效抑制MCF-7细胞中c-myc的表达，诱导MCF-7细胞凋亡。

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siRNA-guided dual-targeting nanocarrier for breast cancer treatment.

Aims: This study aimed to develop a thermoplastic polyurethane-oleic acid-based nanosystem (TPU-Ole NPs) incorporating siRNA and curcumin (CUR) to overcome multidrug resistance in breast cancer by silencing the c-myc gene.

Methods: TPU-Ole and CUR-loaded NPs were prepared via solvent evaporation and coated with poly-L-lysine (PLL) for siRNA attachment. NPs were characterised by dynamic light scattering (DLS) for mean diameter, polydispersity index (PDI), and zeta potential (ZP). Encapsulation (EE) and loading efficiencies (LE) were measured by NanoDrop. Release (pH 5.0; 7.4) and storage stability (pH 7.4) were evaluated using the eppendorf method. siRNA binding was confirmed by agarose gel electrophoresis. Gene silencing and apoptosis were assessed by RT-PCR and flow cytometry.

Results: Mean diameter, PDI, and ZP of NPs were 170 ± 2 nm, 0.011 ± 0.080, and -27.5 ± 0.11 mV. EE and LE were 75 ± 0.12 and 14.2 ± 0.06%. Sustained release and good stability were observed.

Conclusion: siRNA-CUR-NPs efficiently silenced c-myc and induced apoptosis in MCF-7 cells.

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来源期刊

Journal of microencapsulation 工程技术-工程：化工

CiteScore

6.30

自引率

2.60%

发文量

审稿时长

3 months

期刊介绍： The Journal of Microencapsulation is a well-established, peer-reviewed journal dedicated to the publication of original research findings related to the preparation, properties and uses of individually encapsulated novel small particles, as well as significant improvements to tried-and-tested techniques relevant to micro and nano particles and their use in a wide variety of industrial, engineering, pharmaceutical, biotechnology and research applications. Its scope extends beyond conventional microcapsules to all other small particulate systems such as self assembling structures that involve preparative manipulation. The journal covers: Chemistry of encapsulation materials Physics of release through the capsule wall and/or desorption from carrier Techniques of preparation, content and storage Many uses to which microcapsules are put.