Journal of Food Biochemistry最新文献

{"title":"Synergistic Protection of Alpha-Glucosyl Hesperidin and Procyanidin Against Testicular Ischemia-Reperfusion Injury","authors":"Ehsan Zeynali,&nbsp;Ali Soleimanzadeh,&nbsp;Saeed Azizi","doi":"10.1155/jfbc/5634210","DOIUrl":"https://doi.org/10.1155/jfbc/5634210","url":null,"abstract":"<div>\u0000 <p>The present study was designed to examine the protective effects of alpha-glucosyl hesperidin (AGH) and procyanidin (PC) against testicular ischemia/reperfusion (I/R) injury, which is a severe medical condition. Seven groups of male rats were divided into control, torsion/detorsion (T/D), T/D+50 mg/kg PC, T/D+25 mg/kg AGH, T/D+50 mg/kg AGH, T/D+50 mg/kg PC+25 mg/kg AGH, and T/D+50 mg/kg PC+50 mg/kg AGH. The evaluation of testicular tissue, sperm quality, oxidative status, gene activity, and fertility levels after T/D was conducted using various tests. The results showed that sperm motility, viability, and plasma membrane functionality, as well as total antioxidant capacity (TAC), superoxide dismutase (SOD), catalase (CAT), and <i>Bcl-2</i> gene expression levels, all increased after treatment with AGH or PC and coadministration. Additionally, the levels of glutathione (GSH), malondialdehyde (MDA), <i>Bax,</i> and <i>caspase-3</i> gene expression and abnormal morphology and DNA damage decreased compared to the T/D group. The study found that AGH and PC had a more substantial synergistic effect when used together to improve reproductive health following T/D damage.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2025 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/5634210","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143475722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Review on the Extraction Methods, Bioactivities, and Application in Foods of Silk Sericin","authors":"Yijin Wang,&nbsp;Jingxuan Gao,&nbsp;Yemei Yang,&nbsp;Lizhou Zhu,&nbsp;Wei Yang,&nbsp;Pingping Li,&nbsp;Weike Yang,&nbsp;Wen Yang","doi":"10.1155/jfbc/2155701","DOIUrl":"https://doi.org/10.1155/jfbc/2155701","url":null,"abstract":"<div>\u0000 <p>Silk sericin, a byproduct of the silk industry, is typically discarded during degumming in the textile industry. However, recent studies have revealed that sericin possesses various biological activities, including antibacterial, anti-inflammatory, and antioxidative activities; lowers blood sugar; and promotes intestinal health. These activities can be attributed to the rich content of polar amino acids with hydroxyl groups, such as serine and threonine, in the secondary structure of sericin. Additionally, sericin contains eight essential amino acids necessary for the human body that humans cannot synthesize by their own, most get them through food. Thus, sericin is a potential food resource. The ability to utilize sericin as a source of bioactive peptides and amino acids from silk byproducts is very important for fully utilizing silk products, increasing the value of byproducts, and addressing the current low utilization rate of sericin in the food industry. This review provides a summary and analysis of the extraction methods, research activities, and current applications of sericin in the food industry. In addition, the study provides reference information for the effective utilization of surplus resources from silkworm cocoons and the full utilization of sericin.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2025 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/2155701","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143481581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancements in Spray-Drying for the Microencapsulation of Fat-Soluble Vitamins: Stability, Bioavailability, and Applications","authors":"Samira Askari Vaselabadi,&nbsp;Seyed Mohammad Taghi Gharibzahedi,&nbsp;Ralf Greiner,&nbsp;Jim M. Vale,&nbsp;Airemwen Collins Ovenseri,&nbsp;Ali Rashidinejad,&nbsp;Shahin Roohinejad","doi":"10.1155/jfbc/9974476","DOIUrl":"https://doi.org/10.1155/jfbc/9974476","url":null,"abstract":"<div>\u0000 <p><b>Background:</b> Spray-drying is a well-established method for producing free-flowing microencapsulated powders, especially for fat-soluble vitamins (FSVs) like A, D, and E. This process enhances the stability and protection of FSVs against environmental degradation.</p>\u0000 <p><b>Scope and Approach:</b> This review highlights the application of spray-drying to encapsulate FSVs, focusing on their role in maintaining stability, controlled release, and protection under various conditions. Current challenges and future directions in spray-drying-assisted encapsulation of single and multiple FSVs (like vitamin K) are also discussed.</p>\u0000 <p><b>Key Findings and Conclusions:</b> Optimizing the spray-drying technique with efficient encapsulating agents can remarkably improve the release profiles and yields of vitamin A, emphasizing tailored formulations for stability and efficacy. For vitamin D, the choice of carrier oil and wall materials has a significant impact on bioavailability, particularly in food fortification. Although there are challenges due to heat sensitivity, spray-drying is a versatile technique to encapsulate vitamin E with high efficiency rate, desirable particle characteristics, and effective release profiles. Comicroencapsulation with bioactive compounds further enhances the stability and bioavailability of vitamins, suggesting promising applications in food fortification and health supplementation. Comparative studies between microencapsulated FSVs and their free forms revealed improved physicochemical stability and extended-release rates in simulated gastrointestinal conditions, as well as an enhancement in nutrient retention in food products.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2025 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/9974476","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143466160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Skin’s New Shield: Food-Derived Bioactive Peptides in Combating Photoaging—An Investigation Into Inhibitory Mechanisms and Structure–Activity Relationships","authors":"Ruijia Liu,&nbsp;Lijuan He,&nbsp;Linfeng Chen,&nbsp;Yihao Zhang,&nbsp;Yinxiao Zhang,&nbsp;Jiulong An,&nbsp;He Li,&nbsp;Xinqi Liu","doi":"10.1155/jfbc/2836436","DOIUrl":"https://doi.org/10.1155/jfbc/2836436","url":null,"abstract":"<div>\u0000 <p>In recent years, skin health has become one of the leading causes of nonfatal disease burden due to the significance of the skin in the body and the ubiquitous presence of ultraviolet radiation that negatively affects skin conditions and causes skin photoaging. Bioactive peptides (BAP) have received much attention for their antiphotoaging activity in skin because they are natural, safe, and functional. Previous studies have demonstrated that specific amino acids or sequences, as well as some characteristic amino acids, are key to the antiphotoaging effects of BAP. Nevertheless, their inhibitory mechanisms and the association between their structures and abilities are not comprehensively documented. This paper summarizes the mechanisms of antiphotoaging peptides derived from different food processing by-products from the literature over the past 4 years, focusing on the structure–activity relationship based on the inhibitory mechanisms and amino acid compositions of BAP. In addition, the current challenges in obtaining and researching BAP are discussed, and future approaches are proposed. This review provides a theoretical basis for discovering more possible food-borne antiphotoaging peptides and exploring the correlation between the structures and effects of antiphotoaging peptides.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2025 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/2836436","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143466159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Glucose Metabolites in Honey Samples From Diverse Botanical and Elevation Ancestries","authors":"Wed Mohammed Ali Alarjani,&nbsp;Amal Al-Mosa,&nbsp;Rahaf Mohammed Hussein Alshareef,&nbsp;Amani Aed Yahia Laheg,&nbsp;Mohammed Babiker,&nbsp;Hamed A. Ghramh,&nbsp;Mohammed Elimam Ahamed Mohammed","doi":"10.1155/jfbc/5074885","DOIUrl":"https://doi.org/10.1155/jfbc/5074885","url":null,"abstract":"<div>\u0000 <p>Honey is a natural sweet substance composed of major and minor constituents. The major honey constituents include sugars and water, while the minor components include organic acids, proteins, short peptides, enzymes, flavonoids, phenolic acids, minerals, vitamins, dicarbonyls, and hydrogen peroxide. It is evident that the constituents of honey affect each other such as the vice versa relation between methylglyoxal and hydrogen peroxide. This study investigated the relations between vitamin C, hydrogen peroxide, and dicarbonyl concentrations in <i>Ziziphus</i> and <i>Acacia</i> honey samples from diverse floral origins and altitudes in Saudi Arabia. Vitamin C and hydrogen peroxide were assessed by redox titrations, and the dicarbonyl molecules were measured using spectrophotometric method. The obtained data were analyzed using SPSS (Version 21.0). The floral origin and the altitude had significant effects on all the studied parameters except vitamin C. Our findings indicated that when the mean concentration of vitamin C in the Ziziphus honey (576 m) (283.84 ± 116.54 mg/100 g) and the mean concentration of the dicarbonyl molecules (176.4 ± 98.06 mg/kg) were high, the hydrogen peroxide mean concentration was low (3.19 ± 0.18 g/kg). When the hydrogen peroxide concentration in the Ziziphus honey (113 m) was high (6.68 ± 0.23 g/kg), the concentration of vitamin C and the dicarbonyl molecules was low (194.48 ± 16.36 mg/100 g and 55.1 ± 38.09 g/kg, respectively). Thus, the present findings suggested that honey samples rich in dicarbonyl molecule concentration are expected to have a high amount of vitamin C and a low concentration of hydrogen peroxide. The medicinal and nutritional values of honey depend on its active enzymatic and nonenzymatic reactions.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2025 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/5074885","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143456041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Storage Conditions on Phytochemical Quality and Free Radical Scavenging Potential of Nelumbo nucifera Rhizome","authors":"Haq Nawaz,&nbsp;Farwa Mumtaz,&nbsp;Rimsha Aosaf,&nbsp;Vania Amin,&nbsp;Muhammad Yousaf,&nbsp;Mubashir Nawaz,&nbsp;Nafeesa Tahir","doi":"10.1155/jfbc/8369567","DOIUrl":"https://doi.org/10.1155/jfbc/8369567","url":null,"abstract":"<div>\u0000 <p>The current investigation optimized the impact of storage conditions on the phytochemical quality and free radical scavenging potential of <i>Nelumbo nucifera</i> rhizome using a response surface methodology. A bifactorial central composite design (CCD) was constructed at five levels of each of the storage temperatures (<i>S</i>_<i>T</i>: −80, −20, 0, 5, and 10°C) and storage time (<i>S</i>_<i>t</i>: 1, 2, 3, 4, and 5 weeks) consisting of 29 experimental runs with four factorial and four axial points in triplicate and five center points. The rhizome samples were stored at various combinations of <i>S</i>_<i>T</i> and <i>S</i>_<i>t</i> as selected by CCD. The fresh and stored samples were processed to analyze their phytochemical composition and free radical scavenging potential. The stored rhizome samples showed comparatively lower values (<i>p</i> &lt; 0.05) of phytochemical constituents and free radical scavenging potential than the fresh ones. The response surface optimization showed that the samples’ phytochemical content and free radical scavenging potential changed as a linear positive function of <i>S</i>_<i>T</i> and a linear negative function of <i>S</i>_<i>t</i>. A linear positive effect of <i>S</i>_<i>T</i> and a linear negative effect of <i>S</i>_<i>t</i> were observed on the total phenolic content, total flavonoid content, and tannin content, as well as 2, 2-diphenyl-1-picrylhydrazyl, hydroxyl, azino-bis-tetrazolium sulfate cation, and superoxide radical scavenging capacities of <i>N. nucifera</i> rhizome. The optimum levels of <i>S</i>_<i>T</i> and <i>S</i>_<i>t</i> for optimal response of the studied parameters were found to be 8.48°C and 2.0313 weeks, respectively. The study suggests avoiding long-term storage of plant-based foods at very low temperatures to preserve their phytochemical and antioxidant quality.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2025 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/8369567","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143455958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spirostanol-Type Saponins From Allium Macrostemonis Bulbus Alleviate Chronic Unpredictable Mild Stress–Induced Depression-Like Behavior in Mice","authors":"Weixing Ding,&nbsp;Yu Sun,&nbsp;Jianfa Wu,&nbsp;Hongyan Li,&nbsp;Zengfa Wang,&nbsp;Wei Li,&nbsp;Leiling Shi,&nbsp;Jing Zhang,&nbsp;Lulu Wang","doi":"10.1155/jfbc/8113079","DOIUrl":"https://doi.org/10.1155/jfbc/8113079","url":null,"abstract":"<div>\u0000 <p>Major depressive disorder is a chronic, recurrent, and potentially life-threatening mental disorder that severely impacts both psychological and physiological health. Allii Macrostemonis Bulbus (AMB), a traditional Chinese medicinal and edible plant, is believed to be beneficial for mood regulation. Recent preclinical studies have demonstrated its total saponins’ antidepressant effects, though the specific active substances and pathways remain unclear.We first established an <i>in vitro</i> model of hippocampal neuronal injury in mice induced by Glu and conducted preliminary screening of different polarity fractions of AMB’s total saponins, identifying spirostanol-type saponins (AMBN-90) with antidepressant potential. We then analyzed the components and used network pharmacology to predict the targets and pathways of AMBN-90’s antidepressant effects. Next, we established an <i>in vivo</i> mouse model of depression induced by chronic unpredictable mild stress and treated it with AMBN-90 for 6 weeks. Behavioral tests, hippocampal HE staining, and monoamine level measurements in the hippocampus were used to verify AMBN-90’s antidepressant effects. Subsequently, Western blotting and molecular docking methods were employed to preliminarily reveal AMBN-90’s potential antidepressant mechanisms. Results showed that AMBN-90 exhibited significant neuroprotective activity <i>in vitro. In vivo</i>, AMBN-90 significantly improved weight loss and depression-like behaviors in mice exposed to chronic unpredictable mild stress, ameliorated hippocampal neuronal damage, and significantly increased levels of 5-HT, DA, and NE in the hippocampus. It also elevated brain-derived neurotrophic factor levels and reduced neuronal apoptosis by activating the PKA–CREB–BDNF pathway. Molecular docking showed that components of AMBN-90 have superior affinity for the upstream proteins of the pathway, C-type G protein–coupled receptors (GPCRs). Our findings suggest that steroidal saponins in AMB may be developed as functional foods and drugs for alleviating depression.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2025 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/8113079","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143447017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated Analysis of Morphological Alterations, Antioxidant Capacity, and Metabolomic Profiles in Daylily Flower Buds During Storage","authors":"Hongrui Lv,&nbsp;Yongji He,&nbsp;Lina Xu,&nbsp;Shang Guo","doi":"10.1155/jfbc/8651782","DOIUrl":"https://doi.org/10.1155/jfbc/8651782","url":null,"abstract":"<div>\u0000 <p>Daylily flower buds are edible and medical homologous with health care functions and pharmaceutical values. However, they are about to open and deteriorate after harvest, owing to ripening and senescence. To obtain a theoretical reference for understanding the postharvest senescence mechanisms of daylily flower buds, the dynamic quality characteristics of daylily flower buds were explored by physiology, biochemistry, and widely targeted metabolomics. During the whole storage process, texture, total flavonoid contents, and antioxidant activities indicated by DPPH, ABTS, and FRAP assays appeared in declining trends. Based on widely targeted metabolomics, 518, 443, and 507 differentially accumulated metabolites (DAMs) were screened in the initial storage period (CK0 vs. CK2), mid-storage period (CK2 vs. CK4), and the end of storage (CK4 vs. CK6), respectively. A total of 224 common DAMs in CK0 vs. CK2, CK2 vs. CK4, and CK4 vs. CK6 were closely related to the ripening and senescence of daylily. A special focus was given to the interplay between the marker flavonoids and antioxidant activities. 11 flavonoid compounds showed positive correlation with the results of antioxidant capacity, including pelargonidin 3−O−beta−D−glucoside 5−O−(6−coumaroyl−beta−D−glucoside), typhaneoside, 5‴−O−feruloyl complanatoside B, 7−neohesperidosides, taxifolin, rutin (trihydrate), rutin (hydrate), rutin (hydrate), 3,5,7,3′,4′−pentahydroxyflavylium−3−O−beta−D−xylopyranosyl−(1−2)−beta−D−glucopyranoside, kuromanin (chloride), and neoeriocitrin, which have the potential to be biomarkers for antioxidant capacity and daylily quality. The study innovatively served as a valuable guide for the senescence progress and suggested potential markers for the quality characteristics of daylily flower buds.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2025 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/8651782","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143431367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Genoprotective Role of Laurocerasus officinalis: Study on Genotoxic/Antigenotoxic and Cytotoxic/Anticytotoxic Effects in Human Lymphocytes","authors":"Esra Yıldız,&nbsp;Guncha Meredova,&nbsp;Hüseyin Aksoy","doi":"10.1155/jfbc/7455954","DOIUrl":"https://doi.org/10.1155/jfbc/7455954","url":null,"abstract":"<div>\u0000 <p><i>Laurocerasus officinalis</i>, a valuable medicinal plant, has rich flavonoid and anthocyanin content, providing benefits for various diseases. It also has ethnopharmacological uses such as diuretics and antidiabetics. Phenolic compounds exhibit their anticarcinogenic effects directly by repairing DNA damage and reducing chromosomal abnormalities. This study aims to investigate genotoxicity/antigenotoxicity and cytotoxicity/anticytotoxicity of <i>L. officinalis</i> extract in human peripheral lymphocytes by the methods of chromosomal aberration (CA), cytokinesis-block micronucleus (CBMN) test, and mitotic index value. The doses of <i>L. officinalis</i> extract were determined as 125, 250, 500, and 1,000 μg/mL for the genotoxicity test, and mitomycin C (MMC) was added to induce DNA damage for the investigation of antigenotoxicity. In both applications (24 and 48 h), all doses, positive control (MMC) and negative control (sterile dH₂O), were used. In CA and CBMN tests for genotoxicity, no significant differences existed between all doses and the solvent control group (<i>p</i> &gt; 0.05). In antigenotoxic activity, doses added with MMC were significantly lower than positive control (<i>p</i> &lt; 0.05). For both the 24-hour and 48-hour periods, the percentage of abnormal cells and the CAs per cell ratio were significantly lower at all <i>L. officinalis</i> extract concentrations compared to the positive control. Additionally, as the treatment concentration increased, the CA ratio showed a decreasing trend. These differences were strongly dose-dependent compared to the positive control (<i>r</i> = −0.799). In all concentrations in the CBMN test, the frequencies were significantly lower than the positive control, and the higher the <i>L. officinalis</i> extract concentrations, the lower the micronuclei (MN). There was a strong dose-dependent relationship in MN formation compared to positive control (<i>r</i> = −0.925). Therefore, <i>L. officinalis</i> extract has no genotoxic effect on human lymphocytes and also has antigenotoxic and protective effect to the damage of MMC. In the mitotic index result, the <i>L. officinalis</i> extract was cytotoxic at only one dose (125 μg/mL) but anticytotoxic at all other doses against MMC exposure. In conclusion, consuming <i>L. officinalis</i> medicinally benefits and may protect against exposure to genotoxic agents.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2025 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/7455954","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143423801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of Polysaccharide Extraction Technology From Perilla frutescens Leaves and Its α-Glucosidase Inhibition Activity","authors":"Xiaocui Liu,&nbsp;Zhongqiao Li,&nbsp;Lin Xu,&nbsp;Yinzhen Pan,&nbsp;Wenlong Li,&nbsp;Xialin Ran,&nbsp;Zhengyang Meng,&nbsp;Jiaorui Zhang,&nbsp;Yage Xing","doi":"10.1155/jfbc/8749940","DOIUrl":"https://doi.org/10.1155/jfbc/8749940","url":null,"abstract":"<div>\u0000 <p><i>Perilla frutescens</i> (PF) processes medicinal and edible properties, making its flowers, stems, leaves, and fruits versatile and valuable. This study optimizes extraction and evaluates the α-glucosidase inhibitory activity of <i>Perilla frutescens</i> leaf (PFL) polysaccharides. It compared PFL polysaccharides extraction efficiency using hot water, cellulase-assisted, and microwave-assisted extraction. The experimental results demonstrated that the enzymatic extraction method yielded the highest extraction yield, reaching 4.64%. We used response surface methodology to investigate the four main influencing factors to optimize the enzymatic extraction. The optimized conditions resulted in an improved extraction yield of 6.29%. For optimal enzymatic extraction, use a solid-liquid ratio of 1:35 g·mL⁻¹, a temperature of 50°C, a time of 120 min, and an enzyme content of 1500 U/g. This study also investigated the <i>α</i>-glucosidase inhibition by <i>Perilla frutescens</i> leaf polysaccharides (PFLPs). The results confirmed that PFLP exhibited an inhibitory effect on <i>α</i>-glucosidase, indicating their potential to be utilized as <i>α</i>-glucosidase inhibitor. The study found reversible noncompetitive inhibition, providing valuable clues for finding new potential hypoglycemic drugs from natural sources.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2025 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/8749940","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143396831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}