Journal of Food Biochemistry最新文献

{"title":"CircRNA-Centric ceRNA Network Profiling Reveals Potential Targets of Andrographolide in Murine Ulcerative Colitis","authors":"Caijuan Si,&nbsp;Long Shu,&nbsp;Wenjing Cao,&nbsp;Yuliang Feng,&nbsp;Hui Dong,&nbsp;Xingzhen He,&nbsp;Jingjing Yuan,&nbsp;Songtao Li,&nbsp;Feiwei Cao,&nbsp;Qin Zhu,&nbsp;Peifen Zheng","doi":"10.1155/jfbc/3245873","DOIUrl":"https://doi.org/10.1155/jfbc/3245873","url":null,"abstract":"<p>Andrographolide (Andro) is a major bioactive component of Andrographis paniculata, a medicinal and edible plant with anti-inflammatory effects. However, its effects against ulcerative colitis (UC) and circRNA-related mechanism(s) remain unclear. We established the UC mice model by administrating dextran sulfate sodium (DSS) for 14 days. Treatment with Andro for the last 7 days markedly reversed the shortened colon length, decreased the DAI, and abnormal expression of genes related to inflammation and barriers. Besides, sequencing and qRT-PCR revealed circAsxl2 was upregulated in the DSS group while downregulated after Andro treatment. Moreover, knockdown of circAsxl2 corrected gene expression related to inflammation and barriers in lipopolysaccharide-induced HT-29 cells. Then, miRNA and DEmRNAs associated with circAsxl2 were predicted and used to construct a competitive endogenous RNA (ceRNA) network, in which DEmRNAs mRNA was enriched in immune-related pathways by KEGG. Therefore, Andro possesses the potential to serve as a functional food ingredient for the prevention and management of UC.</p>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2026 1","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/3245873","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148462768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Postharvest Application of γ-Aminobutyric Acid as a Natural Bioactive Compound Enhances Quality Retention in Hawthorns During Cold Storage via Activation of Antioxidant Defense Mechanisms","authors":"Md. Arfan Ali,&nbsp;Ayşe Tülin Öz,&nbsp;Sule Polat,&nbsp;Ebru Kafkas","doi":"10.1155/jfbc/6674593","DOIUrl":"https://doi.org/10.1155/jfbc/6674593","url":null,"abstract":"<p>The limited storability of hawthorn fruits is primarily due to rapid metabolic changes that compromise fruit quality. While cold storage is recommended to stall this process, it can induce chilling injury (CI) characterized by pitting. In this study, the impact of <i>γ</i>-aminobutyric acid (GABA) (GABA-0, GABA-5 mM, and GABA-10 mM) on “Sultan” hawthorns stored at 2°C and 90% RH for 120 days was evaluated. Both GABA treatments effectively reduced CI, maintaining fruit quality with greener peels, higher membrane stability index (MSI), and better retention of firmness and titratable acidity (TA), while reducing total soluble solids (TSS) compared to the control. GABA treatments reinforced the antioxidant defense system, increasing activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT), as well as concentrations of total phenol content (TPC), total flavonoid content (TFC), and vitamin C. Treatment with GABA reduced the activities of hydrolytic enzymes and polyphenol oxidase (PPO), as well as microbial counts and decay incidence. The findings indicate that GABA at 10 mM reduced CI by 6% compared with the control (20% after 120 days of cold storage), while also contributing to delayed senescence and improved maintenance of quality attributes in hawthorn fruit.</p>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2026 1","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/6674593","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148386109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Food-Derived Flavonoids Show Predicted Allele-Dependent Interaction Profiles With a Candidate EEF1A2 Codon 361 Alteration Cluster in Intrahepatic Cholangiocarcinoma","authors":"Weiguo Ren,&nbsp;Chibin Pu,&nbsp;Qiang Li,&nbsp;Xuexiu Zhang","doi":"10.1155/jfbc/5432652","DOIUrl":"https://doi.org/10.1155/jfbc/5432652","url":null,"abstract":"<p>Food-derived flavonoids are of increasing interest as bioactive compounds relevant to nutraceutical and functional-food research, but mutation-aware prioritization of such compounds remains limited in intrahepatic cholangiocarcinoma (CCA). Whole-exome sequencing data from seven tumor-normal matched CCA pairs were reanalyzed and integrated with mutation annotation, structural mapping, cell-based assays, molecular docking, and 100-ns molecular dynamics simulations. The cohort showed marked intertumoral heterogeneity, with 324–1244 nonsilent mutations per tumor. A recurrent cluster of EEF1A2 alterations was identified within codons 353–393, including codon 361 substitutions observed in three tumors. Because these codon 361 events involved adjacent nucleotides within the same codon and phasing information was unavailable in the public dataset, the pattern is interpreted conservatively as a candidate recurrent alteration cluster rather than a validated driver hotspot. In HuCCT-1 cells, the ectopic expression of EEF1A2-I361M or EEF1A2-I361L increased proliferation and wound closure relative to wild-type EEF1A2, with I361M showing a stronger phenotype. Structure-guided docking and molecular dynamics simulations further suggested predicted allele-dependent interaction profiles of luteolin, morin, and taxifolin near the mutated region. These computational results are presented as hypothesis-generating prioritization outputs and not as proof of direct target engagement or allele-selective biological activity. Overall, the findings identify EEF1A2 codon 361 as a candidate molecular context for future validation of food-derived flavonoids in CCA-related research.</p>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2026 1","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/5432652","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148386502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ginsenoside Rg1 Counteracts the Negative Effects of a High-Sugar Diet on Female Drosophila melanogaster Reproduction by Modulating Insulin and Steroid Hormone Signaling","authors":"Ying Chen,&nbsp;Rui Ma,&nbsp;Xiaoyu Teng,&nbsp;Pengdi Yang,&nbsp;Baoyu Fu,&nbsp;Ling Yang,&nbsp;Liwei Sun,&nbsp;Yanping Wang,&nbsp;Fangbing Liu","doi":"10.1155/jfbc/7014264","DOIUrl":"https://doi.org/10.1155/jfbc/7014264","url":null,"abstract":"<p>The consumption of high-sugar diets by women of reproductive age can adversely affect reproductive health by inducing hormonal and metabolic imbalance. This study investigated the efficacy of a natural compound, ginsenoside Rg1, in mitigating these negative phenotypes. It was found that prolonged high-sugar intake led to obesity, lipid accumulation, ovarian atrophy, and reproductive disorders in female fruit flies. Treatment with ginsenoside Rg1 significantly alleviated the reproductive damage caused by the high-sugar diet, including observed increases of 59.49% in egg production, 84.48% in pupariation numbers, and 19.68% in pupation success. Ginsenoside Rg1 also prevented sugar/fat accumulation, stabilized insulin levels, elevated the concentrations of 20-hydroxyecdysone (20E), and restored endocrine balance. Mechanistically, Rg1 exhibited cellular target engagement with the insulin receptor (INR), leading to enhanced expression of its downstream extracellular signal-regulated kinase (ERK) and estrogen receptor (ER) and increased insulin signaling. Rg1 also showed cellular target engagement with the steroid hormone receptor (ecdysone receptor [ECR]), boosting ecdysone-induced protein 75B (Eip75B) and broad (Br) expression to activate steroid hormone signaling. These pathways acted synergistically to modulate expression of the yolk protein receptor and vitellogenin, critical for oogenesis. Molecular docking simulations predicted that Rg1 could interact with INR and ECR, a prediction corroborated by CETSA data indicating cellular target engagement. These findings collectively support a mechanistic model in which Rg1 physically engages both receptors to modulate downstream signaling. Alterations in gene expression in INR- and ECR-mutant flies confirmed the mutual influence of insulin and steroid hormone signaling. Ginsenoside Rg1 may thus activate both insulin- and steroid hormone-related signaling pathways to alleviate reproductive damage caused by high-sugar diets. Furthermore, the activation of steroid hormone signaling by Rg1 was dependent on the presence of insulin signals. These findings provide preliminary mechanistic insights and generate testable hypotheses for future investigation into the potential of Rg1 in addressing reproductive disorders associated with long-term excessive sugar intake.</p>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2026 1","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/7014264","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148386863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nontargeted Metabolomics Analysis of Metabolite Characteristics of a Novel Etiolated Cultivar Zhonghuang No. 1 During Green Tea Processing and Its Positive Effects of Glycolipid Metabolism","authors":"Baoming Tian,&nbsp;Yan Shen,&nbsp;Kaili Gao,&nbsp;Jiacheng Ye,&nbsp;Yizhu Pan,&nbsp;Lianbao Zheng,&nbsp;Kai Yang,&nbsp;Xiaolong Ji","doi":"10.1155/jfbc/1467101","DOIUrl":"https://doi.org/10.1155/jfbc/1467101","url":null,"abstract":"<p>Green tea processed from etiolated leaves exhibits a distinctive umami taste and fresh aroma, however, the specific compositional changes in the fresh leaves of etiolated <i>Zhonghuang No. 1</i> during green tea processing—particularly regarding mechanisms of glycolipid metabolism regulation—remain poorly understood. In this study, we began by quantifying the bioactive components of etiolated green tea and then analyzed metabolite dynamics and glycolipid-lowering potential during processing using untargeted metabolomics and in vitro enzyme inhibition assays, respectively. Our key findings indicate that curl-shaped tea (DT) underwent the most substantial metabolic shifts, characterized by marked alterations in sugar and linoleic acid metabolites, alongside rapid accumulation of amino acids, phenylpropanoids, and terpenoids. In contrast, during flower-shaped tea (ST) formation, flavonoids, phenylpropanoids, terpenoids, and amino acids exhibited marked compositional shifts. These results indicate that processing-driven accumulation of flavonoids and terpenoids underpins the hypoglycolipidemic effects, providing mechanistic insights for developing yellow leaf green tea (YLGT) as a functional food regulating glycolipid metabolism.</p>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2026 1","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/1467101","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148386942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and Purification and Chemical Structure Analysis of Ginseng Hemicelluloses With Obesity Inhibitory Activity","authors":"Yanan Gao,&nbsp;Xuemei Fu,&nbsp;Yue Sun,&nbsp;Chengqiang Deng,&nbsp;Mingxing Wang","doi":"10.1155/jfbc/3638976","DOIUrl":"https://doi.org/10.1155/jfbc/3638976","url":null,"abstract":"<p>Obesity has emerged as a growingly severe public health challenge across the globe. Hemicelluloses, as crucial medicinal components of <i>Panax ginseng,</i> exhibit inhibitory activity against obesity. In the study, the inhibition of α-glucosidase activity was used as the index of investigation, and a novel extraction method of ginseng hemicelluloses (GSH1) with obesity inhibitory activity was preferred by single-factor experiments and response surface methodology. Subsequently, the chemical structure of GSH1 was confirmed by GPC, HPLC, FT-IR, and NMR. Finally, the mechanism of action was explored by circular dichroism spectroscopy, fluorescence spectroscopy, and molecular docking simulations. The antiobesity activity of GSH1 was investigated using in vivo and in vitro obesity models. Results indicated that GSH1 is an acidic polysaccharide with a β-D-xylose structure. GSH1 showed significant inhibitory activity to α-glucosidase. Furthermore, GSH1 exhibited significant antiobesity effects. In conclusion, GSH1 holds promise as a natural antiobesity ingredient with potential applications in food and pharmaceutical development.</p>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2026 1","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/3638976","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148324712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis and Characterisation of Water-Soluble β-Carotene–Xylan Complexes","authors":"Rūta Gruškienė,&nbsp;Tatjana Kavleiskaja,&nbsp;Jolanta Sereikaitė","doi":"10.1155/jfbc/3466529","DOIUrl":"https://doi.org/10.1155/jfbc/3466529","url":null,"abstract":"<p>β-Carotene is a useful compound for food fortification because it has beneficial effects on human health. However, it is insoluble in water and sensitive to light, oxygen and temperature. To increase the solubility and stability of β-carotene, its complexation with polysaccharides is used. The study aimed to develop and optimise a new method for β-carotene–xylan (CAR–XYL) complex fabrication. The physical and chemical properties of the resulting complexes were investigated. The β-carotene:xylan ratios of 1:3 (w/w) and 1:5 (w/w) were concluded to be the most applicable to β-carotene formulation. The solubility of the complexes was 36.8 and 38.3 mg/mL, respectively. The particles were small in size. Their hydrodynamic diameters were 300.4 ± 10.4 nm and 242.2 ± 2.6 nm, respectively. The thermal stability of the complexes at different temperatures for 30 min was not significantly affected by the β-carotene:xylan ratio. However, the shelf life of β-carotene under UV irradiation and long-term storage under different conditions showed a tendency to decrease in β-carotene:xylan ratios of 1:10 (w/w) and 1:20 (w/w). β-Carotene complexed with xylan did not lose its antioxidant activity. Furthermore, for the use of the β-carotene–xylan complexes in functional foods, the bioaccessibility of β-carotene complexed with xylan was studied using the INFOGEST protocol. For CAR–XYL complexes fabricated at CAR:XYL ratios of 1:3 and 1:5, bioaccessibility was 54.3 ± 5.6% and 59.03 ± 3.3%, respectively.</p>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2026 1","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/3466529","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148324672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic Effects of Bromelain on Oxidative Stress, Inflammatory Gene Expression, Metabolic Profile, and Renal Injury in STZ-Induced Diabetic Rats","authors":"Fardin Omranzadeh Asl,&nbsp;Arash Karimi,&nbsp;Vahid Asghariazar,&nbsp;Rouhallah Moradpour,&nbsp;Saeede Jalali,&nbsp;Lalhe Fakhr,&nbsp;Mehdi Asghari Vostakolaei","doi":"10.1155/jfbc/6486633","DOIUrl":"https://doi.org/10.1155/jfbc/6486633","url":null,"abstract":"<p>The experimental study aimed to examine the impacts of bromelain on glycemic control, lipid profile, oxidative stress, and inflammatory markers in diabetic rats. Forty male Wistar rats were randomly divided into four groups: control group (<i>n</i> = 10), diabetic group (<i>n</i> = 10), 25 mg/kg bromelain-treated group (<i>n</i> = 10), and 50 mg/kg bromelain-treated group (<i>n</i> = 10). For 8 weeks, the current study examined the potential protective effects of bromelain on rat models of diabetes produced by streptozotocin (STZ) at a dose of 50 mg/kg b.w. Bromelain could significantly improve insulin and HDL-c levels and reduce concentrations of cholesterol, LDL-c, triglycerides, blood urea nitrogen, urea, and creatinine (<i>p</i> &lt; 0.001). In addition, total antioxidant capacity, glutathione peroxidase, and superoxide dismutase levels were noticeably higher, and malondialdehyde levels were significantly lower in the bromelain-treated groups compared with the diabetic group (<i>p</i> &lt; 0.001). Furthermore, treatment with bromelain could significantly reduce the mRNA expression levels of NF-κB, TP53, TNF-α, IL-1β, COX-1 (only in the high-dose group), COX-2, and PI3K compared with the diabetic group (<i>p</i> &lt; 0.01). However, the mRNA expression level of Nrf-2 was significantly higher in the bromelain-treated groups than in the diabetic group (<i>p</i> &lt; 0.001). Treatment with bromelain could improve oxidative stress, glycemic control, lipid profile, and inflammatory markers in diabetic rats.</p>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2026 1","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/6486633","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148324738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary Flavonoids Attenuate Inflammation and Cartilage Matrix Degradation in Experimental Osteoarthritis","authors":"Yuancheng Li,&nbsp;Weiyu Lu,&nbsp;Xin Huang,&nbsp;Jiazhou Li,&nbsp;Lijuan Zou,&nbsp;Huashan Pan","doi":"10.1155/jfbc/9550385","DOIUrl":"https://doi.org/10.1155/jfbc/9550385","url":null,"abstract":"<p>Osteoarthritis (OA) is characterized by progressive cartilage destruction, synovitis, extracellular matrix (ECM) remodeling, and chronic low-grade inflammation. Although multiomics studies have identified numerous OA-associated genes, the translation of these signals into experimentally supported and food-relevant intervention strategies remains incomplete. In this study, public synovial transcriptomic datasets from GEO were integrated to identify differentially expressed genes (DEGs), and genetic support was further examined by two-sample Mendelian randomization (MR) and colocalization analyses using blood eQTL and knee OA GWAS summary statistics. Immune-cell deconvolution, single-cell analysis, cell–cell communication inference, and structure-based docking were used to define the biological context of prioritized signals. To evaluate food-biochemistry-oriented in vivo relevance, the dietary flavonoids quercetin, luteolin, and apigenin were assessed in a monosodium iodoacetate (MIA)–induced mouse OA model using histological assessment, serum inflammatory cytokine assays, and qPCR-based analysis of the SKAP2/MEG3-related inflammatory-catabolic axis. Integrated transcriptomic analysis identified 1503 exploratory OA-associated DEGs, including 793 upregulated and 710 downregulated genes. MR analysis identified 318 genes with nominal genetic association with OA, and intersection with synovial DEGs yielded five candidate genes: SKAP2, TEFM, UROS, MEG3, and SPHK1. Among these candidates, SKAP2 and MEG3 showed the strongest colocalization evidence with OA (PP.H4 = 1.0000 and 0.8019, respectively) and moderate diagnostic performance in the integrated synovial cohort (AUC = 0.764 and 0.721, respectively). Immune infiltration and single-cell analyses placed SKAP2 mainly in immune-cell compartments and MEG3 mainly in structural joint-cell compartments, linking them to an immune-fibrotic and TGF-β-related synovial network. In MIA-induced OA mice, quercetin, luteolin, and apigenin reduced histological cartilage degeneration and synovitis, lowered serum TNF-α, IL-1β, and IL-6 concentrations, suppressed the transcriptional activation of Skap2, Tgfb1, Tgfbr2, Mmp13, and Adamts5, and restored Meg3 expression. Overall, this study identifies SKAP2 and MEG3 as genetically supported and biologically plausible OA-associated signals and provides in vivo evidence that dietary flavonoids can modulate OA-associated inflammatory and catabolic transcriptional programs.</p>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2026 1","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/9550385","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148324645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lymphatic Appearance of Prenylated Coumaric Acids After Gastric Administration in Rats","authors":"Masayuki Yamaga,&nbsp;Hiroko Tani,&nbsp;Kaeko Murota","doi":"10.1155/jfbc/1146776","DOIUrl":"https://doi.org/10.1155/jfbc/1146776","url":null,"abstract":"<p>Phenolic compounds, also known as (poly)phenols, are major phytochemicals characterized by diverse chemical structures. Prenylation, a hydrophobic modification, occurs naturally on various (poly)phenols. Prenylated derivatives of <i>p</i>-coumaric acid, such as drupanin and artepillin C, are characteristically found in Brazilian green propolis (BGP), which is derived from <i>Baccharis dracunculifolia</i> DC. (Asteraceae). However, studies on the bioavailability of prenylated derivatives of (poly)phenols remain limited. In this study, we investigated the intestinal absorption pathways of prenylated derivatives of <i>p</i>-coumaric acid present in BGP using thoracic lymph–cannulated rats. BGP was solubilized in 10% propylene glycol and administered in the stomach of fasting rats. Lymph and peripheral plasma samples were analyzed using liquid chromatography-tandem mass spectrometry. Both the intact compounds and their conjugated metabolites, including glucuronides and sulfates, were detected in the lymph and plasma. At a dose of 300 mg BGP/kg, the lymphatic/peripheral plasma ratios of <i>p</i>-coumaric acid and its monoprenylated form, drupanin, were 21% and 16%, respectively. These ratios increased following the administration of 30 mg/kg. Notably, artepillin C, a diprenylated derivative of <i>p</i>-coumaric acid, exhibited preferential lymphatic transport, with lymphatic/peripheral plasma ratio of 72% at 300 mg BGP/kg. At a 30 mg/kg dose, its concentration in the lymph was 10-fold higher than that in the plasma. The concentration ratios in the lymph and peripheral plasma were correlated to the octanol/water partition coefficients of these compounds, indicating that the addition of prenyl groups increased the hydrophobicity of these compounds and may facilitate their lymphatic transport. In conclusion, diprenylated derivative artepillin C was highly hydrophobic and transported to the lymphatic system after intestinal absorption. These results will contribute to elucidating the pharmacokinetic properties of prenylated derivatives of (poly)phenols.</p>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2026 1","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/1146776","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148324018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}