Journal of Food Biochemistry最新文献

{"title":"Freshness Assessment of Sweetpotatoes Based on Physicochemical Properties and VOCs Using HS-GC-IMS Combined With HS-SPME-GC-MS Analyses","authors":"Xiaowei Wu,&nbsp;Wen Zhang,&nbsp;Zhixin Guo,&nbsp;Jian Zheng,&nbsp;Ping Xu,&nbsp;Xinru Zhang,&nbsp;Mingyi Yang,&nbsp;Yuge Guan,&nbsp;Jiyu Cheng,&nbsp;Guoquan Lu,&nbsp;Linjiang Pang,&nbsp;Xinghua Lu","doi":"10.1155/jfbc/6855678","DOIUrl":"https://doi.org/10.1155/jfbc/6855678","url":null,"abstract":"<div>\u0000 <p>This study investigates the dynamic changes of volatile organic compounds (VOCs) during the storage of sweetpotatoes and their correlation with freshness, providing guidance for quality evaluation. The physicochemical indicators of sweetpotatoes stored at 15°C and 90% relative humidity were analyzed, followed by cluster analysis based on these indicators. VOCs during storage were measured using headspace–gas chromatography–ion mobility spectroscopy (HS-GC-IMS) and headspace–solid-phase microextraction–gas chromatography–mass spectrometry (HS-SPME-GC-MS) techniques. The results showed that the physicochemical properties of sweetpotatoes changed regularly with storage time, allowing for the classification of freshness into three stages. Both HS-GC-IMS and HS-SPME-GC-MS results indicated significant changes in VOCs during storage, with orthogonal partial least squares discriminant analysis (OPLS-DA) effectively distinguishing between different freshness stages. Further multivariate statistical analysis of the HS-SPME-GC-MS data identified benzaldehyde, β-cyclocitral, β-cyclohomocitral, methyl phenylacetate, ethyl phenylacetate, eugenol, and β-ionone as indicators of freshness changes. A prediction model for the freshness variation of sweetpotatoes was constructed based on these VOCs, providing an important reference for analyzing freshness changes by measuring the VOCs released during storage.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2025 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/6855678","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144524507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Intermittent Fasting on Physical Performance and Exercise Outcomes: A Systematic Review and Meta-Analysis of Clinical Trials","authors":"Erfan Mortezapour,&nbsp;Mahsa Ranjbar,&nbsp;Elaheh Dehghani,&nbsp;Zahra Noushzadeh,&nbsp;Sakineh Shab-Bidar,&nbsp;Nagham Jouni,&nbsp;Kurosh Djafarian","doi":"10.1155/jfbc/2856753","DOIUrl":"https://doi.org/10.1155/jfbc/2856753","url":null,"abstract":"<div>\u0000 <p>Intermittent fasting (IF) practices are common eating patterns among physically active population. Therefore, the possible effect of IF on physical activity should be vigorously explored. The objective was to perform a systematic review along with a meta-analysis to determine the possible impacts of IF on physical performance. From the earliest feasible date to January 2024, all of three electronic databases of PubMed, Scopus, and Web of Science were searched thoroughly. Two authors identified studies that evaluated the effect of IF on physical performance. The combined effects were expressed as weighted mean differences (WMDs) and 95% confidence intervals (CIs) implementing a random effect model. The risk of bias in the selected studies was evaluated with the Cochrane Collaboration’s tool. A total of 29 studies met the inclusion criteria. Results elucidated that IF did not exert any significant effect on some physical performance parameters including Wingate peak power output, vertical jump height, maximal heart rate, bench press 1RM, rating of perceived exertion, blood lactate concentration, and handgrip strength. Overall, our findings indicate that IF does not exert a significant impact on key physical performance indicators, including strength, anaerobic capacity, perceived exertion, or cardiovascular response to exercise. However, more studies need to be done in this area incorporating athletes from a wider range of physical fitness and diverse exercise routines.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2025 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/2856753","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144515073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Colla Corii Asini Prevents H2O2-Induced Cellular Aging and Skin Barrier Damage in HaCaT Keratinocytes","authors":"Xinyu Gao,&nbsp;Shuhua Ma,&nbsp;Yanan Sun,&nbsp;Lishuang Li,&nbsp;Yucui Jin,&nbsp;Jianjun Gu,&nbsp;Haibin Liu,&nbsp;Huiyuan Gao,&nbsp;Yi Wang","doi":"10.1155/jfbc/2885165","DOIUrl":"https://doi.org/10.1155/jfbc/2885165","url":null,"abstract":"<div>\u0000 <p><b>Background:</b> Aging weakens the skin barrier, causing dryness and inflammation. <i>Colla Corii Asini</i> (CCA) shows antioxidant and antiaging potential, possibly protecting against aging-related skin damage.</p>\u0000 <p><b>Objective:</b> This study aims to evaluate the effects of CCA on H₂O₂-induced aging and skin barrier damage in human keratinocytes (HaCaT) cells and to explore its underlying mechanisms.</p>\u0000 <p><b>Method:</b> We performed proteomics on CCA, simulated its digestion to obtain CCA intestinal absorption solution (CCA IAS), and tested its therapeutic effects on H₂O₂-induced skin aging and barrier damage in HaCaT cells.</p>\u0000 <p><b>Results:</b> Proteomic analysis of CCA reveals its potential to prevent aging and repair skin barrier damage by regulating key protein domains and biological processes. CCA enhances intestinal absorption of amino acids, hydroxyproline, and proteins, with significant antioxidant capabilities in the absorption fluid. CCA intestinal absorption solution (CCA IAS) enhances the proliferation and migration of H₂O₂-induced HaCaT cells and reduces oxidative stress by regulating reactive oxygen species (ROS) levels and antioxidant enzyme activity. Additionally, CCA reduces gene expression of aging markers p21, p53, and senescence-associated secretory phenotype (SASP). CCA IAS modulates gene expression of differentiation markers FLG, IVL, K10, tight junction proteins CLD-1, ZO-1, E-cadherin, and AMPs, while inhibiting phosphorylation in the mitogen-activated protein kinase (MAPK) pathway.</p>\u0000 <p><b>Conclusion:</b> This study demonstrates that CCA alleviates oxidative stress-induced aging and repairs H₂O₂-induced barrier damage in HaCaT cells, potentially through reducing phosphorylation levels in the MAPK pathway. These findings provide a foundation for further mechanistic and application-focused research on CCA.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2025 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/2885165","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144514647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of Ripening and Fractions on Phenolic Composition and Antioxidant Capacity in Australian Jackfruit (Artocarpus heterophyllus L.)","authors":"Shujun Ye,&nbsp;Ali Imran,&nbsp;Osman Tuncay Agar,&nbsp;Dakshina Yadav,&nbsp;Chelsea Moore,&nbsp;Hafiz A. R. Suleria","doi":"10.1155/jfbc/5212167","DOIUrl":"https://doi.org/10.1155/jfbc/5212167","url":null,"abstract":"<div>\u0000 <p><i>Artocarpus heterophyllus</i> Lam., a tropical tree native to India and prevalent in Asia, Africa and parts of South America, boasts a phytochemical profile dominated by polyphenols. The composition of jackfruit phytochemistry changes during maturity stages due to enzymatic and biochemical reactions. Extraction module and conditions play a crucial role in polyphenol extraction. This investigation aimed to understand the impact of ripening and extraction methods on polyphenol concentration in different jackfruit fractions. Two fresh jackfruit samples, unripe jackfruit (JUR) and ripe jackfruit (JR), along with a commercially frozen sample (JC), were sourced from the local market of Australia. Samples were further processed into four different fractions (peel, core, seed and fruit), and extraction was carried out through conventional and ultrasonic extraction systems. Ripened jackfruit samples exhibited higher phenolic content and antioxidant activity than unripened ones. Among the jackfruit fractions, the highest phenolic contents and antioxidant activity were observed in the peel followed by core, seed and fruit. As far the extraction module is concerned, ultrasound extraction-based jackfruit samples exhibited overall higher phenolic content and antioxidant activity as compared to conventional. The LC–ESI–QTOF–MS/MS screening showed the presence of 65 compounds in different jackfruit samples showing the diversity of phenolics in this fruit. The majority of the compounds belonged to flavonoids (49), lignans (5), phenolic acids (7) and other polyphenols (4). The HPLC quantification showed that the gallic acid, catechin, syringic acid, epicatechin, coumaric acid, trans-ferulic acid, sinapic acid, procyanidin A2, quercetin, caffeic acid and kaempferol were present in an appreciable amount in all the samples. This study provides insight into the changes in the phenolic profile during different ripening stages in different fractions of the jackfruit. These changes may impart a significant impact on the physicochemical and nutritional properties of the jackfruit.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2025 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/5212167","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144473231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Purification, Biochemical Characterization and Digestive Stability Analysis of the Major Allergen Paramyosin From Haliotis discus hannai","authors":"Ling-Jing Zhang,&nbsp;Rui Zou,&nbsp;Le-Chang Sun,&nbsp;Yu-Lei Chen,&nbsp;Kai-Yuan Cao,&nbsp;Ru-Qing Yang,&nbsp;Zhong-Lin Li,&nbsp;Ling Weng,&nbsp;Min-Jie Cao","doi":"10.1155/jfbc/8877354","DOIUrl":"https://doi.org/10.1155/jfbc/8877354","url":null,"abstract":"<div>\u0000 <p>Paramyosin (PM) is a structural protein in invertebrates, playing an important role in maintaining the texture of muscle. Although PM has been identified as a major allergen, its characteristics have not been well investigated. In this study, PM with a molecular mass of 97 kDa was purified to homogeneity from <i>Haliotis discus hannai</i> using ammonium sulfate fractionation and hydroxyapatite column chromatography. Liquid chromatography tandem mass spectrometry (LC–MS/MS) analysis identified 11 peptide fragments with a total of 66 amino acid residues, which were 100% identical to PM from <i>Haliotis discus discus</i>. The sequence of the PM gene, including its complete open reading frame of 2583 bp encoding 860 amino acid residues, was determined by molecular cloning. PM is almost a long-rod chain protein, and α-helix is the dominant secondary structure. Simulated gastrointestinal fluids digestion showed that PM is highly tolerant to digestive proteinases. A specific polyclonal antibody against PM was prepared. Western blot analysis revealed that PM was the most abundant in <i>H</i>. <i>discus hannai</i>, followed by <i>Rapana venosa</i>, <i>Argopecten irradians</i>, and <i>Uroteuthis chinensis</i>, while PM could not be detected in shrimp <i>Penaeus merguiensis</i> and freshwater fish <i>Hypophthalmichthys molitrix</i>, strongly suggesting the unique existence in shellfish.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2025 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/8877354","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144367183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expanding the Application of Potato Starch in Diverse Food Products by Modifying Its Water Absorption, Swelling, and Solubility Through Pregelatinization–Cold Plasma Treatments","authors":"Ali Taghavi,&nbsp;Azadeh Ranjbar Nedamani,&nbsp;Ali Motevali,&nbsp;Seyyed Jafar Hashemi","doi":"10.1155/jfbc/6809100","DOIUrl":"https://doi.org/10.1155/jfbc/6809100","url":null,"abstract":"<div>\u0000 <p>In this study, samples of starch were treated using a cold plasma reactor operated at 20 kV and 40 W. Experimental design was performed with Design Expert software, comprising 27 treatments involving factors such as pregelatinization temperatures (55°C and 60°C), cold plasma exposure times (0, 15, and 30 min), and starch drying temperatures (60°C, 70°C, and 80°C). The results indicated that cold plasma treatment significantly affected the water absorption capacity (WAC) of the starch, with increases attributed to starch depolymerization and the formation of simple sugars that enhance water retention. Fourier transform infrared (FTIR) spectroscopy revealed notable structural modifications, including shifts in absorption bands associated with C–H and C–O stretching, implying changes in molecular bonds and polymer composition, which led to a reduction in starch granule size. Scanning electron microscopy (SEM) images showed surface etching and the formation of porous, fragmented starch granules, contributing to improved moisture absorption and solubility. Overall, cold plasma treatment proved to be an effective and environmentally friendly method for enhancing the functional properties of starch, facilitating its application in food and pharmaceutical industries while improving its structural characteristics.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2025 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/6809100","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144315387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-ROS and Anticancer Potential of Rhizomes and a Polyunsaturated Fatty Acid From Chloroform Fraction of Curcuma wallichii as a Bioactive Compound","authors":"Md. Sabbir Hossain,&nbsp;A. B. M. Ashraful,&nbsp;Aziz Abdur Rahman,&nbsp;Mamunur Rashid,&nbsp;Md. Golam Sadik,&nbsp;A. H. M. Khurshid Alam","doi":"10.1155/jfbc/9517484","DOIUrl":"https://doi.org/10.1155/jfbc/9517484","url":null,"abstract":"<div>\u0000 <p><b>Background and Aim:</b> Cancer incidence and mortality rates remain high despite significant advancements in treatment strategies. This study explores the in vitro anti-ROS activities, in silico anticancer potential of the isolated compound, and in vivo cytotoxicity of chloroform fraction of <i>Curcuma wallichii</i> (CHCW), which is known for diverse pharmacological properties.</p>\u0000 <p><b>Experimental Procedure:</b> The anti-ROS properties were investigated using various in vitro assay techniques, including total antioxidant capacity, ferrous reducing power, DPPH scavenging, hydroxyl radical scavenging, and lipid peroxidation inhibition. Ehrlich Ascites Carcinoma (EAC) cell–induced tumor-bearing mice were used to evaluate the in vivo anticancer potential of the fraction. An <i>in silico</i> study was conducted using a computer-aided model with the P⁵³ protein.</p>\u0000 <p><b>Key Results:</b> The phytochemical screening revealed the presence of a wide variety of plant metabolites. The crude methanolic extract and CHCW exhibited high phenolic and flavonoid content with powerful antioxidant activity. Moreover, CHCW and aqueous fraction showed significant DPPH radical scavenging activity with IC₅₀ values of 15.09 and 19.83 μg/mL, respectively. Both extracts demonstrated the highest inhibitory activity in the brain lipid peroxidation assay with IC₅₀ values of 46.86 and 53.85 μg/mL. Additionally, CHCW resulted in significant tumor growth inhibition of 72.69% and 36.70% at doses of 10 mg and 5 mg/kg body weight, respectively. The isolated methyl linoleate yielded a docking score of −4.6 kcal/mol, suggesting potential anticancer activity. The toxicological study indicated that the compound exhibited no side effects.</p>\u0000 <p><b>Conclusions and Implications:</b> The CHCW fraction exhibited potent anti-ROS and tumor cytotoxicity in all assays performed. Therefore, <i>C</i>. <i>wallichii</i> might be a promising source for the development of cancer therapeutics.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2025 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/9517484","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144309163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Limosilactobacillus fermentum XY18 Relieves CCl4-Induced Acute Liver Injury in Mice by Antioxidant and Anti-Inflammatory","authors":"Ranran Wang,&nbsp;Liping Yang,&nbsp;Aijuan Xie,&nbsp;Ruokun Yi,&nbsp;Yongpeng He,&nbsp;Xin Zhao,&nbsp;Zhaofeng Tian","doi":"10.1155/jfbc/9085638","DOIUrl":"https://doi.org/10.1155/jfbc/9085638","url":null,"abstract":"<div>\u0000 <p>This study investigated whether <i>Limosilactobacillus fermentum</i> XY18 (LF-XY18) prevents carbon tetrachloride (CCl₄)-induced mice acute liver injury (ALI) by alleviating oxidative stress and inflammation. LF-XY18 reduced liver weight, improved injury indices, and alleviated histological abnormalities in mice. LF-XY18 significantly reduced serum alanine triglycerides (TG), aspartate aminotransferase (AST), total cholesterol (TC), and aminotransferase (ALT) levels and reduced the myeloperoxidase (MPO) and malondialdehyde (MDA) concentrations in both serum and liver tissues, while increasing glutathione (GSH) and superoxide dismutase (SOD) levels in these compartments. Moreover, LF-XY18 activated the nuclear factor-erythroid-2-related-factor-2 (Nrf2) antioxidant pathway, upregulating heme oxygenase-1 (HO-1), copper-zinc superoxide dismutase (Cu/Zn-SOD), manganese-superoxide dismutase (Mn-SOD), and glutathione peroxidase (GSH-Px). Simultaneously, LF-XY18 modulated the nuclear factor κ-light-chain-enhancer of activated B cells (NF-κB)-mediated inflammatory responses, restoring the NF-κB, B-cell inhibitor-α (IκB-α), neuronal nitric oxide synthase (nNOS), endothelial NOS (eNOS), inducible NOS (iNOS), and cyclooxygenase-2 (COX-2) expressions. LF-XY18 dose-dependently ameliorated CCl₄-induced ALI through oxidative stress and inflammation suppression, demonstrating probiotic properties suitable for development as hepatoprotective dietary supplements.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2025 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/9085638","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144309160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Betulinic Acid–Mediated NR1D1 Alleviates Dextran Sulfate Sodium–Induced Colitis in Mice by Modulating VDAC1/NF-κВ","authors":"Qikun Zhang,&nbsp;Ge Song,&nbsp;Rulei Zhong,&nbsp;Haibo Wang,&nbsp;Zhiqiang Liu,&nbsp;Haodong Cui,&nbsp;Jimin Yin,&nbsp;Yong Qi,&nbsp;Wenyong Wu","doi":"10.1155/jfbc/3244032","DOIUrl":"https://doi.org/10.1155/jfbc/3244032","url":null,"abstract":"<div>\u0000 <p><b>Purpose:</b> Inflammatory bowel disease (IBD) can severely disrupt intestinal health, leading to chronic inflammation. Betulinic acid (BHA) has demonstrated anti-inflammatory and antioxidant properties. This study aims to explore the mechanism by which BHA alleviates colitis through the regulation of the key circadian gene REV-ERBα (NR1D1) in a DSS-induced colitis mouse model.</p>\u0000 <p><b>Methods:</b> The study first utilized <i>in vivo</i> experiments to establish a mouse model of colitis induced by DSS, investigating the effect of BHA in alleviating colitis through the regulation of NR1D1. Subsequently, an inflammatory model was established at the cellular level using CCD841 cells treated with 100 ng/mL LPS to explore the regulatory mechanism of BHA on colitis.</p>\u0000 <p><b>Results:</b> Our results indicate that oral administration of BHA effectively alleviates colitis symptoms, as shown by reduced disease activity index and histopathological scores. Notably, we found that BHA improves intestinal inflammation in DSS-induced mice by downregulating VDAC1/NF-κB. In addition, <i>in vitro</i> experiments demonstrate that the anti-inflammatory effects of BHA are closely related to the NR1D1 gene.</p>\u0000 <p><b>Conclusion:</b> Our findings demonstrate the potential of BHA as a preventive and therapeutic agent for IBD.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2025 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/3244032","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144281619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination of Eight Catechins and Four Theaflavins in Qingzhuan Dark Tea of Different Years (1, 5, 9, and 13 Years)","authors":"Xiaolei Miao,&nbsp;Yiye Zhao,&nbsp;Ping Luo,&nbsp;Dan Ye,&nbsp;Chen Xie,&nbsp;Lanru Tang,&nbsp;Min Zheng","doi":"10.1155/jfbc/1214060","DOIUrl":"https://doi.org/10.1155/jfbc/1214060","url":null,"abstract":"<div>\u0000 <p>Catechins and theaflavins are the key polyphenolic compounds in Qingzhuan dark tea (QDT). They not only give QDT unique taste and color but also have significant health benefits such as antioxidant, anti-inflammatory, and cardiovascular protection, which are the core of the quality and healthcare value of QDT. However, the variation of its content is closely related to the vintage of the tea. The aim of this study was to develop a rapid, sensitive, and accurate high-performance liquid chromatography–tandem mass spectrometry (HPLC-MS/MS) method for quantifying eight catechins and four theaflavins in QDT of different years (1, 5, 9, and 13 years). An orthogonal experimental design was employed to determine the optimal extraction conditions. Under these conditions, QDT samples from different years (1, 5, 9, and 13 years) were extracted, and eight catechins and four theaflavins were quantified using the established HPLC-MS/MS method. The results showed that the optimal extraction conditions for methanol were determined as follows: methanol concentration of 60%, temperature of 90°C, and extraction time of 15 min, while for water extraction, the optimal conditions were temperature of 90°C and extraction time of 30 min. Methodological validation of the eight catechins and four theaflavins was conducted, including specificity, linearity, lower limit of quantitation, precision, accuracy, stability, and reproducibility. Different extraction methods (methanol extraction and water extraction) significantly influenced the content of eight catechins and four theaflavins, with higher content observed with methanol extraction compared to water extraction. Significant differences were also observed in the content of eight catechins and four theaflavins among QDT samples of different years (1, 5, 9, and 13 years), with higher years associated with lower content. Conclusion: The method established in this study is reliable, accurate, and reproducible, and could be used to evaluate QDT of different years by quantifying the content of eight catechins and four theaflavins.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2025 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/1214060","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144256212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}