Journal of Food Biochemistry最新文献

{"title":"Expanding the Application of Potato Starch in Diverse Food Products by Modifying Its Water Absorption, Swelling, and Solubility Through Pregelatinization–Cold Plasma Treatments","authors":"Ali Taghavi,&nbsp;Azadeh Ranjbar Nedamani,&nbsp;Ali Motevali,&nbsp;Seyyed Jafar Hashemi","doi":"10.1155/jfbc/6809100","DOIUrl":"https://doi.org/10.1155/jfbc/6809100","url":null,"abstract":"<div>\u0000 <p>In this study, samples of starch were treated using a cold plasma reactor operated at 20 kV and 40 W. Experimental design was performed with Design Expert software, comprising 27 treatments involving factors such as pregelatinization temperatures (55°C and 60°C), cold plasma exposure times (0, 15, and 30 min), and starch drying temperatures (60°C, 70°C, and 80°C). The results indicated that cold plasma treatment significantly affected the water absorption capacity (WAC) of the starch, with increases attributed to starch depolymerization and the formation of simple sugars that enhance water retention. Fourier transform infrared (FTIR) spectroscopy revealed notable structural modifications, including shifts in absorption bands associated with C–H and C–O stretching, implying changes in molecular bonds and polymer composition, which led to a reduction in starch granule size. Scanning electron microscopy (SEM) images showed surface etching and the formation of porous, fragmented starch granules, contributing to improved moisture absorption and solubility. Overall, cold plasma treatment proved to be an effective and environmentally friendly method for enhancing the functional properties of starch, facilitating its application in food and pharmaceutical industries while improving its structural characteristics.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2025 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/6809100","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144315387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-ROS and Anticancer Potential of Rhizomes and a Polyunsaturated Fatty Acid From Chloroform Fraction of Curcuma wallichii as a Bioactive Compound","authors":"Md. Sabbir Hossain,&nbsp;A. B. M. Ashraful,&nbsp;Aziz Abdur Rahman,&nbsp;Mamunur Rashid,&nbsp;Md. Golam Sadik,&nbsp;A. H. M. Khurshid Alam","doi":"10.1155/jfbc/9517484","DOIUrl":"https://doi.org/10.1155/jfbc/9517484","url":null,"abstract":"<div>\u0000 <p><b>Background and Aim:</b> Cancer incidence and mortality rates remain high despite significant advancements in treatment strategies. This study explores the in vitro anti-ROS activities, in silico anticancer potential of the isolated compound, and in vivo cytotoxicity of chloroform fraction of <i>Curcuma wallichii</i> (CHCW), which is known for diverse pharmacological properties.</p>\u0000 <p><b>Experimental Procedure:</b> The anti-ROS properties were investigated using various in vitro assay techniques, including total antioxidant capacity, ferrous reducing power, DPPH scavenging, hydroxyl radical scavenging, and lipid peroxidation inhibition. Ehrlich Ascites Carcinoma (EAC) cell–induced tumor-bearing mice were used to evaluate the in vivo anticancer potential of the fraction. An <i>in silico</i> study was conducted using a computer-aided model with the P⁵³ protein.</p>\u0000 <p><b>Key Results:</b> The phytochemical screening revealed the presence of a wide variety of plant metabolites. The crude methanolic extract and CHCW exhibited high phenolic and flavonoid content with powerful antioxidant activity. Moreover, CHCW and aqueous fraction showed significant DPPH radical scavenging activity with IC₅₀ values of 15.09 and 19.83 μg/mL, respectively. Both extracts demonstrated the highest inhibitory activity in the brain lipid peroxidation assay with IC₅₀ values of 46.86 and 53.85 μg/mL. Additionally, CHCW resulted in significant tumor growth inhibition of 72.69% and 36.70% at doses of 10 mg and 5 mg/kg body weight, respectively. The isolated methyl linoleate yielded a docking score of −4.6 kcal/mol, suggesting potential anticancer activity. The toxicological study indicated that the compound exhibited no side effects.</p>\u0000 <p><b>Conclusions and Implications:</b> The CHCW fraction exhibited potent anti-ROS and tumor cytotoxicity in all assays performed. Therefore, <i>C</i>. <i>wallichii</i> might be a promising source for the development of cancer therapeutics.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2025 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/9517484","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144309163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Limosilactobacillus fermentum XY18 Relieves CCl4-Induced Acute Liver Injury in Mice by Antioxidant and Anti-Inflammatory","authors":"Ranran Wang,&nbsp;Liping Yang,&nbsp;Aijuan Xie,&nbsp;Ruokun Yi,&nbsp;Yongpeng He,&nbsp;Xin Zhao,&nbsp;Zhaofeng Tian","doi":"10.1155/jfbc/9085638","DOIUrl":"https://doi.org/10.1155/jfbc/9085638","url":null,"abstract":"<div>\u0000 <p>This study investigated whether <i>Limosilactobacillus fermentum</i> XY18 (LF-XY18) prevents carbon tetrachloride (CCl₄)-induced mice acute liver injury (ALI) by alleviating oxidative stress and inflammation. LF-XY18 reduced liver weight, improved injury indices, and alleviated histological abnormalities in mice. LF-XY18 significantly reduced serum alanine triglycerides (TG), aspartate aminotransferase (AST), total cholesterol (TC), and aminotransferase (ALT) levels and reduced the myeloperoxidase (MPO) and malondialdehyde (MDA) concentrations in both serum and liver tissues, while increasing glutathione (GSH) and superoxide dismutase (SOD) levels in these compartments. Moreover, LF-XY18 activated the nuclear factor-erythroid-2-related-factor-2 (Nrf2) antioxidant pathway, upregulating heme oxygenase-1 (HO-1), copper-zinc superoxide dismutase (Cu/Zn-SOD), manganese-superoxide dismutase (Mn-SOD), and glutathione peroxidase (GSH-Px). Simultaneously, LF-XY18 modulated the nuclear factor κ-light-chain-enhancer of activated B cells (NF-κB)-mediated inflammatory responses, restoring the NF-κB, B-cell inhibitor-α (IκB-α), neuronal nitric oxide synthase (nNOS), endothelial NOS (eNOS), inducible NOS (iNOS), and cyclooxygenase-2 (COX-2) expressions. LF-XY18 dose-dependently ameliorated CCl₄-induced ALI through oxidative stress and inflammation suppression, demonstrating probiotic properties suitable for development as hepatoprotective dietary supplements.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2025 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/9085638","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144309160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Betulinic Acid–Mediated NR1D1 Alleviates Dextran Sulfate Sodium–Induced Colitis in Mice by Modulating VDAC1/NF-κВ","authors":"Qikun Zhang,&nbsp;Ge Song,&nbsp;Rulei Zhong,&nbsp;Haibo Wang,&nbsp;Zhiqiang Liu,&nbsp;Haodong Cui,&nbsp;Jimin Yin,&nbsp;Yong Qi,&nbsp;Wenyong Wu","doi":"10.1155/jfbc/3244032","DOIUrl":"https://doi.org/10.1155/jfbc/3244032","url":null,"abstract":"<div>\u0000 <p><b>Purpose:</b> Inflammatory bowel disease (IBD) can severely disrupt intestinal health, leading to chronic inflammation. Betulinic acid (BHA) has demonstrated anti-inflammatory and antioxidant properties. This study aims to explore the mechanism by which BHA alleviates colitis through the regulation of the key circadian gene REV-ERBα (NR1D1) in a DSS-induced colitis mouse model.</p>\u0000 <p><b>Methods:</b> The study first utilized <i>in vivo</i> experiments to establish a mouse model of colitis induced by DSS, investigating the effect of BHA in alleviating colitis through the regulation of NR1D1. Subsequently, an inflammatory model was established at the cellular level using CCD841 cells treated with 100 ng/mL LPS to explore the regulatory mechanism of BHA on colitis.</p>\u0000 <p><b>Results:</b> Our results indicate that oral administration of BHA effectively alleviates colitis symptoms, as shown by reduced disease activity index and histopathological scores. Notably, we found that BHA improves intestinal inflammation in DSS-induced mice by downregulating VDAC1/NF-κB. In addition, <i>in vitro</i> experiments demonstrate that the anti-inflammatory effects of BHA are closely related to the NR1D1 gene.</p>\u0000 <p><b>Conclusion:</b> Our findings demonstrate the potential of BHA as a preventive and therapeutic agent for IBD.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2025 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/3244032","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144281619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination of Eight Catechins and Four Theaflavins in Qingzhuan Dark Tea of Different Years (1, 5, 9, and 13 Years)","authors":"Xiaolei Miao,&nbsp;Yiye Zhao,&nbsp;Ping Luo,&nbsp;Dan Ye,&nbsp;Chen Xie,&nbsp;Lanru Tang,&nbsp;Min Zheng","doi":"10.1155/jfbc/1214060","DOIUrl":"https://doi.org/10.1155/jfbc/1214060","url":null,"abstract":"<div>\u0000 <p>Catechins and theaflavins are the key polyphenolic compounds in Qingzhuan dark tea (QDT). They not only give QDT unique taste and color but also have significant health benefits such as antioxidant, anti-inflammatory, and cardiovascular protection, which are the core of the quality and healthcare value of QDT. However, the variation of its content is closely related to the vintage of the tea. The aim of this study was to develop a rapid, sensitive, and accurate high-performance liquid chromatography–tandem mass spectrometry (HPLC-MS/MS) method for quantifying eight catechins and four theaflavins in QDT of different years (1, 5, 9, and 13 years). An orthogonal experimental design was employed to determine the optimal extraction conditions. Under these conditions, QDT samples from different years (1, 5, 9, and 13 years) were extracted, and eight catechins and four theaflavins were quantified using the established HPLC-MS/MS method. The results showed that the optimal extraction conditions for methanol were determined as follows: methanol concentration of 60%, temperature of 90°C, and extraction time of 15 min, while for water extraction, the optimal conditions were temperature of 90°C and extraction time of 30 min. Methodological validation of the eight catechins and four theaflavins was conducted, including specificity, linearity, lower limit of quantitation, precision, accuracy, stability, and reproducibility. Different extraction methods (methanol extraction and water extraction) significantly influenced the content of eight catechins and four theaflavins, with higher content observed with methanol extraction compared to water extraction. Significant differences were also observed in the content of eight catechins and four theaflavins among QDT samples of different years (1, 5, 9, and 13 years), with higher years associated with lower content. Conclusion: The method established in this study is reliable, accurate, and reproducible, and could be used to evaluate QDT of different years by quantifying the content of eight catechins and four theaflavins.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2025 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/1214060","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144256212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pharmacological Appraisal of Phyllanthus emblica in Letrozole-Induced Polycystic Ovarian Syndrome Female Rats Model","authors":"Jiangyu Han,&nbsp;Majid Manzoor,&nbsp;Rida Siddique,&nbsp;Hina Manzoor,&nbsp;Subhan Moazzam,&nbsp;Rizwan Rashid Bazmi,&nbsp;Liaqat Hussain","doi":"10.1155/jfbc/8015756","DOIUrl":"https://doi.org/10.1155/jfbc/8015756","url":null,"abstract":"<div>\u0000 <p>Polycystic ovarian syndrome (PCOS) is a complex endocrinological condition affecting women of reproductive age. It may lead to insulin resistance, hyperandrogenism, obesity, cardiovascular issues, and menstrual irregularities. This study aimed to evaluate the ethanolic extract of <i>Phyllanthus emblica</i> (EEPE) against letrozole-induced PCOS in adult female rats. Phytochemical analysis revealed the presence of kaempferol, caffeic acid, quercetin, and ferulic acid. Thirty female rats were first divided into two groups: Group I (normal control, <i>n</i> = 5) and a disease control or PCOS group (<i>n</i> = 25). PCOS was induced using letrozole (1 mg/kg, p.o.), the diseased animals were further divided into four groups: Group II (PCOS control, <i>n</i> = 5) and Groups III–VI (treatment groups, <i>n</i> = 5). The induction of the disease was confirmed through weight gain and vaginal smear cytology. After study completion, female rats were euthanized and blood samples were collected for hormonal evaluation and biochemical analysis. Ovaries were dissected and preserved for histopathological study. Study findings revealed that PCOS significantly elevated the insulin, testosterone, and LH levels. Meanwhile, it significantly reduced the FSH level. Similarly, it also aggravated oxidative stress with reduced levels of SOD, CAT, GSH, and higher levels of lipid peroxidation marker (MDA). Treatment with EEPE elevated FSH levels and reduced the elevated levels of insulin and testosterone. PCOS induction increased the number of cystic follicles in the ovary. It reduced the no. of Graafian and mature follicles, while treatment with EEPE reduced cystic follicles and made it possible to mature these cystic follicles into secondary and Graafian follicles. It also improved the oxidation status of ovaries, which resulted in a good impact on ovulation. In conclusion, this study provided pharmacological validation for the efficacy of EEPE in managing PCOS. Its novelty lies in being the first to scientifically authenticate the traditional use of <i>Phyllanthus emblica</i> for PCOS, offering evidence-based support for its folkloric uses.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2025 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/8015756","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144244807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antioxidant Activity of Pressafonin-A Isolated From Sicilian Ferulago nodosa (L.) Boiss. Roots and Its Proapoptotic Effect Through ROS-Dependent p53/p21Waf1/Cip1 Pathway in Human Melanoma A-375 Cells","authors":"Ignazio Restivo,&nbsp;Natale Badalamenti,&nbsp;Maurizio Bruno,&nbsp;Ilenia Concetta Giardina,&nbsp;Alessandro Massaro,&nbsp;Mario Allegra,&nbsp;Luisa Tesoriere,&nbsp;Alessandro Attanzio","doi":"10.1155/jfbc/8353971","DOIUrl":"https://doi.org/10.1155/jfbc/8353971","url":null,"abstract":"<div>\u0000 <p><i>Ferulago nodosa</i> (L.) Boiss. is a species belonging to the Apiaceae family which grows spontaneously in the Balkan and southern Tyrrhenian areas of the Mediterranean Sea. Plants of the <i>Ferulago</i> genus are widely used in the ethnopharmacological field, mainly in the form of infusions, as remedies against panic attacks, to solve problems related to the gastrointestinal tract, and for ulcer complications. In this study, we assessed antioxidant and antiproliferative activities of the pressafonin-A (PA), extracted from <i>F. nodosa</i> roots, following its spectroscopical characterization. Among the assays conducted, reducing capacity tests and free radical scavenging assays showed that PA can act as an antioxidant. PA also exhibited time- and concentration-dependent antiproliferative activity against human cancer cell lines, including A-375 (melanoma), HCT-116 (colorectal carcinoma), and MCF-7 (breast adenocarcinoma). Selectivity index (SI) was calculated using normal 16-HBE cells. Among the cell lines tested, PA demonstrated an SI &gt; 10 in A-375 cells. Treatment with PA 6.2 μM (IC₂₅) and 12.4 μM (IC₅₀) for 24-h induced apoptosis, as cytofluorimetrically evidenced by phosphatidylserine exposure, loss of mitochondrial membrane potential, and intracellular ROS production. Moreover, PA caused an arrest of cell cycle at the G1/S phase transition. Western blot analysis showed that PA caused an increase in protein levels of p53, p21^Waf1/Cip1, cytochrome c, and BAX, along with a decrease in Bcl-2 protein. In conclusion, natural PA demonstrated antioxidant and antiproliferative properties on A-375 by inducing apoptosis via ROS-dependent intrinsic mitochondrial pathway and cell cycle arrest. These results suggest that PA may have potential as a chemopreventive agent for skin diseases.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2025 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/8353971","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144244712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of Bovine Serum Albumin on the Antioxidant and Anti-Inflammatory Activities of Arbutus unedo L. Extract","authors":"Idir Moualek,&nbsp;Hamdi Bendif,&nbsp;Karima Benarab,&nbsp;Fehmi Boufahja,&nbsp;Natale Badalamenti,&nbsp;Maurizio Bruno,&nbsp;Stefania Garzoli,&nbsp;Karim Houali","doi":"10.1155/jfbc/9947897","DOIUrl":"https://doi.org/10.1155/jfbc/9947897","url":null,"abstract":"<div>\u0000 <p>This study explored the impact of bovine serum albumin (BSA) on the antioxidant and anti-inflammatory activities of <i>Arbutus unedo</i> leaf extract and its polyphenolic compounds. HPLC-MS analysis identified compounds like quinic acid, arbutin, and flavonoids. Antioxidant activity was evaluated using ferric reducing power, phosphomolybdenum, and DPPH assays, while anti-inflammatory effects were assessed via red blood cell membrane stability. BSA enhanced hydroxyl radical scavenging but reduced ferric reducing power and total antioxidant capacity, with no significant anti-inflammatory improvement. The findings reveal assay-dependent effects of BSA due to protein–polyphenol interactions, emphasizing the need for further research to optimize therapeutic potential.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2025 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/9947897","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144244808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling the Metabolic Function of Three Types of High-Temperature Daqu During Baijiu Fermentation","authors":"Shanqimuge,&nbsp;Ping Tang,&nbsp;Minsha Qiao,&nbsp;Fan Wang,&nbsp;Ying Guo,&nbsp;Li Wang,&nbsp;Rongyu Bi,&nbsp;Shuyue Hao,&nbsp;Xinyue Kang,&nbsp;Liqin Qin,&nbsp;Changwen Li,&nbsp;Jun Lu","doi":"10.1155/jfbc/6762405","DOIUrl":"https://doi.org/10.1155/jfbc/6762405","url":null,"abstract":"<div>\u0000 <p>White, yellow, and black Daqu are three typical types of Jiangxiangxing high-temperature Daqu, which result from differences in the microenvironment during Daqu culture. However, there is still limited research on the metabolic characteristics of three types of Daqu and their impact on the final products. In this work, headspace solid-phase microextraction coupled with gas chromatography–mass spectrometry (HS-SPME-GC-MS) and high-throughput sequencing were utilized to reveal volatile compounds and microbial genera in different types of Daqu. Furthermore, simulated fermentation of different types of Daqu was employed to verify their fermentation performance. The results revealed 64 representative volatile compounds and 21 differential microbial genera among three types of Daqu. The correlation analysis and prediction of microbial functional potential indicated that white Daqu was characterized by significant decomposition and utilization of bio-macromolecules, while yellow Daqu had more pronounced formation of aroma compounds. The content of relevant metabolites related to fatty acid and phenylalanine metabolism was significantly higher in yellow Daqu than in the other types. The results of the simulated fermentation of three types of Daqu demonstrated that all types of Daqu stably maintained their functional diversity during fermentation. Specifically, yellow Daqu promoted the production of alcohols and aldehydes, while white and yellow Daqu tended to generate esters and nitrogen-containing compounds such as pyrazines, whereas black and yellow Daqu produced more acids and ketones during fermentation. This study provides a theoretical basis for the rational use of different types of Daqu in Baijiu production.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2025 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/6762405","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144244155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Abutilon indicum (L.) Sweet Extracts Inhibit Key Glucose Metabolic Enzymes While Enhancing Glucose Transport in L6 Myotubes and 3T3L1 Adipocytes","authors":"Subhadip Banerjee,&nbsp;Pravaree Phuneerub,&nbsp;Wuttichai Jaidee,&nbsp;Narawadee Rujanapun,&nbsp;Thidarat Duangyod,&nbsp;Kulwadee Malee,&nbsp;Wisanu Maneerat,&nbsp;Virayu Suthiphasilp,&nbsp;Surat Laphookhieo,&nbsp;Salfarina Ramli,&nbsp;Siau Hui Mah,&nbsp;Kitthisak Khlaeo Chansukh,&nbsp;Poonsit Hiransai,&nbsp;Panupong Puttarak,&nbsp;Satyajit D. Sarker,&nbsp;Lutfun Nahar,&nbsp;Rawiwan Charoensup","doi":"10.1155/jfbc/8252812","DOIUrl":"https://doi.org/10.1155/jfbc/8252812","url":null,"abstract":"<div>\u0000 <p><b>Background:</b> <i>Abutilon indicum</i> (L.) Sweet (Malvaceae) is a traditional medicinal plant known for its antidiabetic properties in Ayurveda and other health systems.</p>\u0000 <p><b>Aims:</b> This study aims to profile metabolites in <i>Abutilon indicum</i> (L.) Sweet extracts (AI) and elucidate their antidiabetic mechanisms through bioinformatics and experimental methods.</p>\u0000 <p><b>Study Design:</b> The ethanolic (AIE) and aqueous (AIA) extracts were evaluated for their inhibitory effects on <i>α</i>-glucosidase and <i>α</i>-amylase, as well as their impact on glucose metabolism in 3T3-L1 adipocytes and L6 skeletal muscle cells. AIE was characterized via HPLC-DAD-QTOF-MS, with network pharmacology and molecular docking analyses used to explore molecular targets.</p>\u0000 <p><b>Methods:</b> In vitro assays were performed to assess enzyme inhibition, and cell line studies HPLC-DAD-QTOF-MS were utilized for compound characterization. Network pharmacology and molecular docking were conducted to reveal underlying antidiabetic mechanisms.</p>\u0000 <p><b>Results:</b> LC-MS-QTOF analysis identified gallic acid, stigmasterol, and riboflavin as abundant compounds. The AIE exhibited significant <i>α</i>-glucosidase (IC₅₀ = 74.15 ± 1.61 μg/mL) and <i>α</i>-amylase inhibition (IC₅₀ = 13.41 ± 0.71 μg/mL). Moreover, it enhanced glucose consumption in 3T3-L1 cells (IC₅₀ = 6.25 μg/mL) and promoted glucose uptake in L6 myotubes. Network pharmacology analyses highlighted the PI3K–Akt signaling pathway’s role in facilitating glucose transport.</p>\u0000 <p><b>Conclusion:</b> The phytochemicals in AIE may contribute significantly to its antidiabetic effects, particularly through the modulation of glucose transport via the PI3K–Akt pathway. Future studies should focus on the preclinical development of safe herbal formulations utilizing these mechanisms for effective diabetes management.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2025 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/8252812","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144244576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}