Journal of Food Biochemistry最新文献

{"title":"Nebulized Lycopene Attenuates Radiation-Induced Pulmonary Inflammation and Fibrosis in Mice via Activating Nrf2/HO-1 and Inhibiting TGF-β1/Smad Signaling","authors":"Zhiming Cui,&nbsp;Hui Zhang,&nbsp;Jiaying Liu,&nbsp;Na Li,&nbsp;Jibo Hu,&nbsp;Yuchao Sun","doi":"10.1155/jfbc/6970441","DOIUrl":"https://doi.org/10.1155/jfbc/6970441","url":null,"abstract":"<p>The development of radiation-induced lung injury (RILI) presents a major clinical challenge that limits the efficacy and safety of thoracic radiotherapy. This condition evolves from acute inflammatory damage to irreversible fibrotic remodeling, yet effective and targeted treatment options remain scarce. Lycopene, a potent dietary carotenoid predominantly found in tomatoes, possesses a multifaceted pharmacological profile encompassing antioxidant, anti-inflammatory, and antifibrotic effects. The nebulized delivery of lycopene offers a direct route to the lungs, enhancing site-specific action and reducing off-target risks, which addresses a key need in managing RILI. This study explored the protective efficacy and mechanisms of nebulized lycopene in RILI. C57BL/6 mice subjected to 20 Gy thoracic irradiation received lycopene via nebulization as preventive and continuous treatment. BEAS-2B and NIH-3T3 cells were treated with irradiation and lycopene in combination with pharmacological inhibitors (ML385 and SB431542) or specific gene knockdown (siNrf2 and siTGF-β1) to validate pathway dependency. Lung injury, inflammation, fibrosis, oxidative stress, and protein expression were detected by histology, ELISA, Western blot, immunofluorescence, and ROS detection. Lycopene dose-dependently improved RILI mice’s survival, alleviated lung damage, inhibited inflammation at 4 weeks and fibrosis at 12 weeks, activated the Nrf2/Heme Oxygenase-1 (HO-1) pathway in BEAS-2B cells, and suppressed the TGF-β1/Smad pathway in NIH-3T3 cells, with good safety. These findings suggest that nebulized lycopene attenuates RILI through differential modulation of epithelial Nrf2/HO-1 signaling and fibroblast TGF-β1/Smad signaling, supporting its candidacy as a dietary bioactive compound for radioprotection.</p>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2026 1","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/6970441","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149146270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeting Metabolic Syndrome With Isoleucine, Lysine and Serine: A Combined In Vitro and Computational Evaluation of Antioxidant and Antidiabetic Inhibitory Effects","authors":"Samuel Idowu Fayomi,&nbsp;Sunday Akintunde Onaolapo,&nbsp;Aimen Aljoundi,&nbsp;Ghazi Elamin,&nbsp;Ochuko Lucky Erukainure,&nbsp;Nontokozo Zimbili Msomi","doi":"10.1155/jfbc/6632434","DOIUrl":"https://doi.org/10.1155/jfbc/6632434","url":null,"abstract":"<p>Diabetes mellitus is the eighth leading health challenge globally, and its management has become a major concern. Research is now focusing on naturally derived organic compounds that can reduce the activity of enzymes involved in carbohydrate and lipid metabolism to manage Type 2 diabetes. The present study combines in vitro and in silico methods to determine the radical-scavenging ability and enzyme-inhibitory capacity of pure isoleucine, lysine and serine. Antioxidant capacity was determined using the DPPH, hydroxyl radical scavenging assay and the ferric reducing antioxidant power (FRAP) assay. The inhibitory effect of α-amylase and α-glucosidase assays was assessed, while their kinetic mode of inhibition was determined using the Michaelis–Menten model and complemented with the Lineweaver–Burk plot. Molecular docking and molecular dynamics were performed to evaluate the binding stability and flexibility of the complexes formed by these enzymes with amino acids. Toxicity prediction and drug-likeness profiling were also performed to predict their safety and drug-like behaviour. In antioxidant assays, the amino acids showed increased radical-scavenging activity in the DPPH and hydroxyl radical assays, but weak reducing power in the FRAP assay. In vitro, isoleucine, lysine and serine showed maximum inhibitions of 72.6%, 72.92% and 76.76%, respectively, against α-amylase. In the α-glucosidase assay, isoleucine, lysine and serine also demonstrated maximum inhibitory effects of 43.57%, 43.66% and 46.55%, respectively. The kinetic behaviour analysis suggested that acarbose, lysine and serine inhibited α-amylase via a mixed inhibition, whereas isoleucine was noncompetitive. In α-glucosidase, isoleucine, lysine and serine showed mixed inhibition, while acarbose exhibited competitive inhibition. Molecular docking and molecular dynamics simulation analyses showed that acarbose exhibited the strongest affinities, stability and flexibility toward α-amylase and α-glucosidase, while isoleucine, lysine and serine displayed weaker affinities and stability. The toxicity prediction provides a variable acute oral toxicity and organ-specific toxicity alert on isoleucine, lysine and serine, with favourable drug-likeness properties. These results revealed that isoleucine, lysine and serine affect multiple pathways relevant to diabetes. This supports their potential as nutraceuticals for managing Type 2 diabetes if it is deeply explored. Further animal studies are required to determine their efficiency and safety in vital organs under physiological conditions.</p>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2026 1","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/6632434","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149145903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive Genomic and Transcriptomic Analysis of the Metabolic Pathway of D-Arabitol Biosynthesis in Debaryomyces hansenii FBKL2.0130","authors":"Xiaodan Wang,&nbsp;Guojun Zhu,&nbsp;Li Yin,&nbsp;Xiaoye Luo,&nbsp;Shaopei Tang,&nbsp;Ke Shen,&nbsp;Anliang Lei,&nbsp;Shidong Ban,&nbsp;Pei Li","doi":"10.1155/jfbc/2508822","DOIUrl":"https://doi.org/10.1155/jfbc/2508822","url":null,"abstract":"<p>D-arabitol is a naturally occurring polyol that is extensively utilized in the food, pharmaceutical, and chemical industries. <i>Debaryomyces hansenii</i> FBKL2.0130, an industrial yeast strain isolated from Moutai-flavor Daqu, demonstrates high glucose tolerance and is capable of producing substantial amounts of D-arabitol. In this study, we observed that the growth curves of FBKL2.0130 demonstrate its capacity to proliferate under various glucose concentrations, with logarithmic growth phases occurring at 48 h and the highest D-arabitol accumulation detected at a glucose concentration of 150 g/L. Furthermore, we conducted the first genomic and transcriptomic analysis of the key metabolic pathways associated with D-arabitol biosynthesis in FBKL2.0130. Genomic analysis revealed that the predominant functional category is metabolism, suggesting that FBKL2.0130 possesses a robust capability for carbohydrate metabolism. Comparative transcriptomic analysis indicated that carbon metabolism and secondary metabolite biosynthesis pathways are the primary contributors to D-arabitol production in FBKL2.0130. This study provides a foundational theoretical basis for understanding the metabolic synthesis of D-arabitol by this yeast strain.</p>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2026 1","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/2508822","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149145901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring Alternative Protein Sources Toward Sustainable and Future Nutrition","authors":"Urvashi Vikranta,&nbsp;Dinesh Chandra Rai,&nbsp;Ankita Hooda,&nbsp;Abhishek Dutt Tripathi,&nbsp;Dipendra Kumar Mahato,&nbsp;Pradeep Kumar,&nbsp;Aparna Agarwal","doi":"10.1155/jfbc/7462906","DOIUrl":"https://doi.org/10.1155/jfbc/7462906","url":null,"abstract":"<p>The growing world population is predicted to cause the need for protein to rise sharply in the upcoming years. Approximately one billion individuals worldwide do not have access to a diet that gives them enough energy and protein. New efforts are being made to enhance the production of high-quality, inexpensive, sustainable, and functional protein sources in order to meet this need. These sources can partially replace those that are traditional protein products, such as meat, caseins, whey proteins, and gelatin. Consumption of an animal-based protein diet possesses many disadvantages, including higher use of resources and higher emission of greenhouse gases, adverse effects on human health, sustainability, and food security. Alternatives to a conventional protein–based diet include plant-based, cell-based, culture-based, and insect-protein–based diets. This review discusses these four alternatives, the markets, and consumer acceptability of these alternatives, which is increasing as consumers now understand the benefits and the drawbacks associated with conventional protein sources. The controlled production of these sources, the participation of regulatory bodies, and appropriate manufacturing procedures all need a great deal of work. Additionally, customers need to be made aware that these new sources are safe, healthful, and suitable for human use as food. The intent of this review is to improve the food system’s sustainability. Alternative plant-based protein research and development can expand dietary options while addressing environmental and human health concerns. Many organizations are exploring newer possibilities of producing, processing, and promoting animal-free protein diets as these are promising alternatives for the upcoming health-conscious consumers.</p>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2026 1","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/7462906","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149146696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ellagitannin Profiling, Antioxidant Activity, and BACE1 Inhibitory Potential of Pomegranate Fruit (Punica granatum L.) Peel and Juice: A Comparative Study","authors":"Tebalelo Lehlaleroa,&nbsp;Chika I. Chukwuma,&nbsp;Nomonde Mapasa,&nbsp;Muriel Mari,&nbsp;Samson S. Mashele,&nbsp;Claudia Ntsapi-Combrink","doi":"10.1155/jfbc/1145605","DOIUrl":"https://doi.org/10.1155/jfbc/1145605","url":null,"abstract":"<p>Alzheimer’s disease (AD) is the leading cause of dementia worldwide and remains incurable due to its multifactorial pathophysiology. <i>Punica granatum</i> L. (pomegranate) has attracted interest as a functional food with neuroprotective potential; however, comparative evidence on the bioactivity of its peel and juice remains limited. This study comparatively evaluated the phytochemical composition, antioxidant capacity, and β-secretase (BACE1) inhibitory activity of aqueous extracts and ellagitannin-rich fractions from pomegranate peel and juice. Total phenolic and flavonoid contents were determined using Folin–Ciocalteu and aluminum chloride assays, respectively. Antioxidant activity was assessed using ferric reducing antioxidant power (FRAP), 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH), and inhibition of H₂O₂-induced reactive oxygen species (ROS) generation in Chang cells. BACE1 inhibition was also evaluated, and phytochemicals were characterized by liquid chromatography–mass spectrometry (LC–MS). The peel ellagitannin-rich fraction exhibited the highest phenolic (555.3 ± 12.3 mg GAE/g) and flavonoid (77.8 ± 3.6 mg QE/g) contents and strongest antioxidant activity, with ABTS and DPPH IC₅₀ values of 6.5 ± 1.3 and 34.8 ± 8.7 μg/mL, respectively. LC–MS identified abundant ellagitannins, including punicalagin. The fraction significantly reduced H₂O₂-induced ROS generation (IC₅₀ = 7.0 μg/mL), comparable to ascorbic acid (8.6 μg/mL; <i>p</i> &gt; 0.05). Peel extract and fraction also inhibited BACE1 activity. Overall, pomegranate peel, particularly its ellagitannin-rich fraction, could be promising nutraceuticals for mitigating the damage caused by oxidative stress and amyloid-beta peptides in neurodegenerative diseases.</p>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2026 1","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/1145605","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149048520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Total Flavonoids From the Dried Root of Tetrastigma hemsleyanum Diels et Gilg Exert Antiacne Properties via Inhibiting Caspase-3 and NF-κB","authors":"Hua Huang,&nbsp;Xinyuan Hu,&nbsp;Yufeng Wang,&nbsp;Lin Tan,&nbsp;Jiale Miao,&nbsp;Junyu Zhou,&nbsp;Xianhao Xu,&nbsp;Haoran Li,&nbsp;Ming Jiang,&nbsp;Dawei Weng,&nbsp;Weihua Yan,&nbsp;Sheng Zhu,&nbsp;Jianbin Zou,&nbsp;Zhida Ke,&nbsp;Jingyu Duan,&nbsp;Chunping Zhang","doi":"10.1155/jfbc/7990354","DOIUrl":"https://doi.org/10.1155/jfbc/7990354","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Acne vulgaris (AV) is one of the most common chronic inflammatory diseases of the skin. The dried root of <i>Tetrastigma hemsleyanum</i> Diels et Gilg contains abundant flavonoid constituents and has anti-inflammatory effects. The current research aims to investigate the antiacne properties of total flavonoids from the dried root of <i>T. hemsleyanum</i> Diels et Gilg (THF) and the related mechanisms in vivo and in vitro.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>THF was extracted, and the constituents were analyzed by UPLC/Q-TOF-MS. Male SD rats were randomly divided into NC, ACNE, and VA groups (0.5 g retinoic acid cream) and THF low, medium, and high groups (5, 10, and 20 mg/mL). Hematoxylin–eosin (HE) staining was used to evaluate right ear auricle injury. RAW 264.7 cells were induced by lipopolysaccharide (LPS) to evaluate the effect of THF on inflammation in vitro. Proteins related to inflammation were evaluated by western blotting.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>In vivo experiments have shown that THF could effectively inhibit the expression of IL-1<i>β</i>, TNF-<i>α</i>, and caspase-3 in oleic acid (OA) and <i>Propionibacterium acnes</i> (<i>P. acnes</i>)–induced acne models in the ears of male SD rats, as well as alleviate ear inflammation of rats. Furthermore, THF inhibited the expression of TNF-<i>α</i>, IL-1<i>β</i>, IL-6, caspase-3, and NF-κB in LPS-stimulated RAW 264.7 cells in vitro.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>THF exerted antiacne properties through alleviating the inflammatory response, and the mechanism may be related to inhibiting the expression of caspase-3 and NF-<i>κ</i>B. This study provides a foundational basis for developing THF aimed at antiacne applications in cosmetics.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2026 1","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/7990354","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149048099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive Phytochemical Characterisation and Integrated In Vitro, In Vivo and In Silico Evaluation of the Antidiabetic Potential of Urtica dioica Leaf Extract","authors":"Kamera Elalem,&nbsp;Ahmed Hariri,&nbsp;Salah Neghmouche Nacer,&nbsp;Elhafnaoui Lanez,&nbsp;Mohammed Larbi Benamor,&nbsp;Anouar Feriani,&nbsp;Ouafa Zouari Ahmed,&nbsp;Touhami Lanez,&nbsp;Stefania Garzoli","doi":"10.1155/jfbc/3687114","DOIUrl":"https://doi.org/10.1155/jfbc/3687114","url":null,"abstract":"<p><i>Urtica dioica</i> L. (stinging nettle) is traditionally used in the management of metabolic disorders, although integrated experimental and computational evaluation of its antidiabetic potential remains limited. This study investigated the phytochemical profile, α-amylase inhibitory activity, in vivo antidiabetic effects and predicted molecular interactions of an ethanolic leaf extract. Targeted triple-quadrupole UPLC–ESI–MS/MS profiling yielded 16 retained MRM signals, with proline accounting for 93.96% of the summed MRM peak area. This percentage represents relative detector response and does not reflect the absolute concentration or mass fraction of proline. The extract inhibited α-amylase in a concentration-dependent manner, with an IC₅₀ of 7.98 ± 0.14 mg/mL compared with 95.37 ± 3.56 μg/mL for acarbose. In alloxan-induced diabetic rats, administration of the extract at 100 mg/kg/day for 28 days reduced fasting blood glucose and HbA1c and improved ALT and AST levels under the experimental conditions employed. Because only one therapeutic dose was evaluated, an efficacy dose–response relationship and optimal therapeutic dose cannot be established. Molecular docking, molecular dynamics simulations, MM–PBSA calculations and ADMET predictions provided complementary evidence of potentially favourable interactions for selected constituents, particularly oleuropein. Computational results for curcumin are considered exploratory because its phytochemical assignment remains tentative. Overall, the findings support further investigation of <i>U. dioica</i> as a source of bioactive constituents with potential relevance to diabetes management, while quantitative metabolite analysis, dose–response studies and direct mechanistic validation remain necessary.</p>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2026 1","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/3687114","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149047970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling the Nutritional and Antioxidant Potential of Various Landraces of Underutilized Legume Horse Gram From Uttarakhand Himalaya","authors":"Pooja Oli,&nbsp;Shailaja Punetha,&nbsp;Vineet Kaswan,&nbsp;Prabhakar Semwal","doi":"10.1155/jfbc/3962533","DOIUrl":"https://doi.org/10.1155/jfbc/3962533","url":null,"abstract":"<p>In India, rice and wheat are staple crops growing majorly in fertile and irrigated land, while legumes often grow in marginal soil and rainfed areas. Horse gram (<i>Macrotyloma uniflorum</i>) is an underutilized legume with significant nutritional potential. However, the variability among native horse gram landraces of Uttarakhand at the landrace level remains insufficiently characterized under controlled and identical conditions. Therefore, the study was conducted using 15 local landraces from different regions of Uttarakhand, which were cultivated under uniform agronomic conditions to assess their biochemical and nutritional diversity. Thereafter, the mature seeds were analyzed for proximate composition and phytochemicals. Proximate analysis revealed that protein, carbohydrate, fat, and ash ranged from 5.2 ± 0.05 to 29.4 ± 0.30 g/100 g dry weight, 49.7 ± 0.26 to 85.3 ± 0.34 g/100 g dry weight, 1.50 ± 0.2 to 5.0 ± 0.0%, and 2.00 ± 0.0 to 4.50 ± 0.0%, respectively, while polyphenols, flavonoid, and anthocyanin were in the range of 3.69 ± 0.2 to 8.64 ± 0.2 mg GAE/g dry weight, 2.23 ± 0.4 to 10.90 ± 0.4 mg QE/g dry weight, and 0.13 ± 0.04 to 2.52 ± 0.02 mg/g dry weight, respectively. Results revealed that landraces H6 and H7 exhibited relatively higher phytochemical content and antioxidant activity, while H9 showed higher protein levels. Correlation analysis indicates positive associations between protein and antioxidant traits, as well as trade-offs between primary nutrients and secondary metabolites. Overall, this study highlights the significance and potential of Uttarakhand’s native horse gram landraces as valuable sources of nutritious and antioxidant-rich food.</p>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2026 1","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/3962533","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148993948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Downregulation of PRKCB Underlies the Gastroprotective Potency of Atractylodes Chinensis Polysaccharides Against Aging","authors":"Kaiyue Li,&nbsp;Yang Liu,&nbsp;Chunhan Qiao,&nbsp;Kaiting Yang,&nbsp;Yidan Chen,&nbsp;Zhumeng Liu,&nbsp;Shuai Ma,&nbsp;Tian Zhang,&nbsp;Yu Cao,&nbsp;Chunying Zhao","doi":"10.1155/jfbc/7790061","DOIUrl":"https://doi.org/10.1155/jfbc/7790061","url":null,"abstract":"<p>Aging disrupts demographic structure and raises health burden. Gastric aging is a major driver of systemic age-related decline, yet targeted interventions remain limited. <i>Atractylodes chinensis</i> demonstrates evident gastric protective effects. The present study investigated the protective mechanism of its active component, <i>Atractylodes chinensis</i> polysaccharides (ACP), in the stomach under aging conditions. ACP treatment reduced apoptosis (from 31.99% to 17.61%) in the gastric tissue of senescence-accelerated mice and rebalanced inflammatory signaling through modulation of CCR1, IL-16, and IRAK3. Integrated multiomics analysis revealed that ACP coordinately rectified the expression of a network of aging-associated proteins (including GNAI2, RYR2, and NDUFV3) and identified the downregulation of PRKCB (protein kinase C beta) as a central regulatory event resisting gastric aging under ACP intervention. Computational analysis indicated that ACP components, including glucuronic acid, glucose, galactose, and arabinose, simultaneously engaged PRKCB and the E3 ubiquitin ligase RNF114, suggesting a potential glue-like mode that may contribute to PRKCB ubiquitination and proteasomal clearance. The downregulation of PRKCB attenuated downstream PRKCB-driven hallmark cellular aging phenotypes and reactive oxygen species accumulation (from 46.88% to 20.35%). Furthermore, ACP reversed the oncogenic aging factors CARM1, OPA1, RAD21, and SNRPD3, whose dysregulation was concurrently validated in clinical gastric tumor transcriptomes, indicating action at a convergent node of aging and tumorigenesis. These findings elucidate a novel antiaging strategy in which natural plant polysaccharides act as targeted degraders of a key aging kinase. This work positions ACP as a promising prototype for mechanistically informed interventions against age-related gastrointestinal dysfunction and its associated pathologies.</p>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2026 1","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/7790061","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148993966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Kombucha Innovation Ecosystem: Bibliometric and Patent Landscape Analysis of Scientific and Technological Trends","authors":"Amanda Felipe Reitenbach,&nbsp;Rômulo Alves Morais,&nbsp;Aryane Ribeiro de Oliveira,&nbsp;Ana Carolina Broch,&nbsp;Taís Suhre,&nbsp;Lucia Alvarez,&nbsp;Munique Gonçalves Guimarães,&nbsp;Eduardo de Souza Mello Falcão,&nbsp;Dario Ellia,&nbsp;Grace Ferreira Ghesti","doi":"10.1155/jfbc/1404058","DOIUrl":"https://doi.org/10.1155/jfbc/1404058","url":null,"abstract":"<p>Kombucha has become one of the fastest-growing fermented functional beverages worldwide, driven by its complex microbial ecology, bioactive composition, sensory diversity and biotechnological potential. This study presents an integrated overview of the global kombucha landscape, combining bibliometric analysis, patent prospection, and critical discussion of microbiological, chemical, sensory and technological aspects. Publications indexed in the Scopus database reveal a sharp increase in scientific output after 2020, focusing on SCOBY dynamics, bioactive compounds, bacterial cellulose, fermentation processes and sensory characterization. Brazil, China, India and the United States stand out as major contributors. In parallel, patent analysis shows accelerated technological development, particularly in Asia, highlighting growing industrial interest in functional beverages, microbial biotechnology, biomaterials and sustainability. The convergence between scientific production and patent activity indicates an effective translation of research into innovation. The review also examines the role of raw materials such as teas, fruits, herbs, and agroindustrial by-products in shaping microbial composition, antioxidant capacity, volatile compounds and sensory attributes. Organic acids, esters, terpenes and phenolic compounds are key drivers of kombucha’s characteristic flavour and aroma. Additionally, process variables including substrate composition, sugar source, SCOBY structure, inoculation strategy, fermentation conditions, and oxygen availability strongly influence acidification, ethanol formation and sensory consistency. Overall, kombucha is evolving from a traditional beverage into a multidisciplinary platform that integrates food science, biotechnology, sustainability and functional product innovation. The review further organizes the health-related literature by the level of scientific evidence, separating in vitro assays, animal models and human clinical trials and shows that controlled human evidence is still restricted to a small number of short trials with few participants. Safety, regulatory and quality control aspects are addressed, including ethanol formation and the 0.5 percent threshold that separates nonalcoholic from alcoholic classification, refermentation during storage, contamination risks and the different risk profiles of industrial and domestic production. Sensory perception, consumer acceptance and the environmental and economic dimensions of by-product valorization complete the analysis.</p>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2026 1","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/1404058","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148943380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}