Journal of Food Biochemistry最新文献

{"title":"A Complete Genome Analysis of Toxic Compound Gene Families in the Genus Cordyceps","authors":"Yingling Lu,&nbsp;Songyu Li,&nbsp;Zuoheng Liu,&nbsp;Yue Chen,&nbsp;Yufan Yin,&nbsp;Quanying Dong,&nbsp;Guodong Niu,&nbsp;Baomin Fan,&nbsp;Hong Yu","doi":"10.1155/jfbc/9371774","DOIUrl":"https://doi.org/10.1155/jfbc/9371774","url":null,"abstract":"<div>\u0000 <p>To evaluate the safety of drug and food homologous properties of <i>Cordyceps</i> species at the genomic level, the complete genomes of 18 <i>Cordyceps</i> strains were sequenced and annotated. Subsequently, their genomic features were compared with those of an additional six <i>Cordyceps</i> strains, and their major secondary metabolites were analyzed using antiSMASH and local BLAST. The results demonstrated significant variations in genome size and GC content among different <i>Cordyceps</i> species, while closely related species exhibited minor differences in these parameters. As wild-type strains, the 24 strains of <i>Cordyceps</i> are likely to lack toxin-encoding genes. <i>C. cateniobliqua</i> YFCC 8434, <i>C. javanica</i> YFCC 8439, and <i>C. javanica</i> IJ1G are expected to serve as novel resources for food ingredients. The genomes of the remaining 21 strains may harbor gene clusters responsible for one or more potentially toxic compounds that could cause morbidity or mortality in the host. This study provides a comprehensive assessment of the safety of potential toxins from <i>Cordyceps</i> fungi at the genomic level, offering a theoretical basis for the development and utilization of novel drug homologous strains.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2025 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/9371774","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144171568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potency of Antioxidant Peptides Encrypted in Commercially Important Marine Invertebrates: A Bioinformatics Approach","authors":"Andreas Vratsistas,&nbsp;Dimitris Vafidis","doi":"10.1155/jfbc/5692584","DOIUrl":"https://doi.org/10.1155/jfbc/5692584","url":null,"abstract":"<div>\u0000 <p>Efficient and beneficial food utilization calls for sustainable and innovative practices that add value at every stage of production. In this study, we conducted a computational analysis of protein sequences from the bivalve <i>Mytilus galloprovincialis</i>, the crustacean <i>Penaeus vannamei</i> (whiteleg shrimp), the purple sea urchin <i>Paracentrotus lividus</i>, and the echinoderm <i>Holothuria leucospilota</i>, utilizing bioinformatic tools to estimate their antioxidant bioactive potential. This potential is indicated by the presence of small bioactive peptides encrypted within their sequences, which can be released by proteolytic enzymes. The peptides of interest from a total of 47 proteins were matched with known antioxidant peptides (APs) from the BIOPEP database. We studied the properties of 236 different peptides out of a total of 995 peptides encrypted in the proteins. To evaluate the bioactivity of peptides, we sorted them based on the predicted free radical scavenging (FRS) and ion-chelating (CHEL) scores. Furthermore, to investigate protein–peptide interactions, molecular docking of selected peptides was performed with two protein models. <i>Penaeus vannamei</i> and <i>Paracentrotus lividus</i> exhibited the highest average antioxidant FRS potency. The most potent FRS peptides for each organism are encrypted in the kinesin-like Boursin protein of <i>Paracentrotus lividus</i>, the Chitinase protein of <i>Penaeus vannamei</i>, the Myosin-16 protein of <i>Holothuria leucospilota</i>, and the Perlucin-like protein of <i>Mytilus galloprovincialis</i>. We demonstrate a correlation between the potency of APs, based on the FRS score, and their calculated binding affinity to serine/threonine protein kinase D1 and glutathione peroxidase. Our results highlight an effective approach to utilizing by-products of primary production for mining high-value ingredients.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2025 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/5692584","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144140821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the Role of Nutraceuticals in the Management and Prevention of Experimental-Induced Abdominal Aortic Aneurysms: Mechanisms and Therapeutic Potential","authors":"Yi Yang,&nbsp;Kun Chen,&nbsp;Jun Chen,&nbsp;Sima-Sadat Sabihi","doi":"10.1155/jfbc/3780766","DOIUrl":"https://doi.org/10.1155/jfbc/3780766","url":null,"abstract":"<div>\u0000 <p>A potentially fatal ailment, abdominal aortic aneurysms (AAAs) frequently result in death by dissection or rupture. AAA is caused by a histopathologic anomaly that includes smooth muscle cell loss, media degeneration, inflammatory cell infiltration, and elastic fiber damage in the aorta wall. Nevertheless, it is still unknown how AAAs form. Improved knowledge of how AAA develops and progresses might lead to the development of innovative, less intrusive treatment approaches for those suffering from this debilitating illness. Functional foods have a range of protective impacts, such as antioxidant, anti-inflammatory, antifibrotic, anticarcinogenic, and cardiovascular protective impacts, as shown by numerous human and animal research. Pretreatment with nutraceuticals can suppress the growth of AAA and pro-inflammatory markers. The use of nutraceuticals to treat ischemic-reperfusion cardiac cells has been shown to protect against ischemia–reperfusion damage through a diversity of mechanisms. This information was most recently reported. Thus, by inhibiting apoptosis, oxidative damage, c-Jun N-terminal kinase pathway, and inflammation, we investigated the impact of nutraceuticals on experimental-induced AAA in the current review.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2025 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/3780766","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144135739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Attenuating Potential of Ginger (Zingiber officinale) Against Fluoride and Dimethoate-Induced Oxidative Stress–Associated Hepatotoxicity in Wistar Rats","authors":"Priyanka Sharma,&nbsp;Pawan Kumar Verma,&nbsp;Shilpa Sood,&nbsp;Sheen Tukra,&nbsp;Bhawani Singh,&nbsp;Gholamreza Abdi,&nbsp;Zuhaib F. Bhat","doi":"10.1155/jfbc/4776314","DOIUrl":"https://doi.org/10.1155/jfbc/4776314","url":null,"abstract":"<div>\u0000 <p>The chronic intake of fluoride (F)-contaminated groundwater, clinically manifested as fluorosis, is a significant disease burden worldwide. Similarly, dimethoate (DM), a broad-spectrum organophosphorus insecticide, is one of the most indiscriminately used insecticides in many parts of the world, posing health risks to exposed humans and animals. In the present subacute study, adult Wistar rats were exposed to DM (1/10th LD₅₀) and F (4.5 ppm) in drinking water alone and in combination to evaluate the effect of coexposure on hepatic parenchyma and to evaluate the attenuating potential of <i>Zingiber officinale</i> (ZO) extract against this combined toxicity. The coexposure to the toxicants significantly (<i>p</i> &lt; 0.05) increased levels of MDA (146.35 ± 11.79) and AOPP (2.85 ± 0.27) and reduced TAS (12.98 ± 0.65), TTH (1.06 ± 0.24), and activities of CAT (1301.12 ± 77.84), SOD (263.59 ± 9.69), GPx (109.02 ± 2.97), GR (18.12 ± 1.24), and AChE (9684.13 ± 526.84) in hepatic tissues compared to control. These observations further corroborated with the significantly (<i>p</i> &lt; 0.05) increased activities of plasma hepatic biomarkers (alanine and aspartate aminotransferase, total plasma proteins, and albumin). Similarly, the liver of the coexposed rats suffered significant (<i>p</i> &lt; 0.05) histopathological damage. However, the alterations in antioxidant and biochemical biomarkers and histopathological changes were greater in the coexposed group. Concurrent supplementation of hydroalcoholic extract of ZO rhizome, a widely used home remedy, significantly (<i>p</i> &lt; 0.05) reduced the combined hepatotoxic effects in Wistar rats.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2025 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/4776314","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144125975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of Microbial Community Succession and Flavor Formation During Fermentation in Chinese Northern Strong-Flavor Daqu","authors":"Peng Xiao,&nbsp;Silei Lv,&nbsp;Ling Xu,&nbsp;Yunran Shen,&nbsp;Fengguo Zhang,&nbsp;Youqiang Xu,&nbsp;Bowen Wang,&nbsp;Hehe Li","doi":"10.1155/jfbc/9279456","DOIUrl":"https://doi.org/10.1155/jfbc/9279456","url":null,"abstract":"<div>\u0000 <p>The aroma of strong-flavor Daqu is a key quality indicator, but its aroma components remain incompletely understood. These compounds stem from microbial metabolism, yet research on northern strong-flavor Daqu microbiota remains scarce. This study utilized physicochemical analyses, gas chromatography-olfactometry-mass spectrometry (GC-O-MS) detection, and Illumina high-throughput sequencing to comprehensively investigate the fermentation process of northern strong-flavor Daqu. Results indicated that both the diversity and contents of volatile compounds increased during fermentation, generating nutty, woody, and roasted aroma notes. A total of 105 volatile substances were identified, of which 21 were identified as the key aroma-active compounds, including ethyl hexanoate, hexanal, and 2,3,5-trimethylpyrazine. Dominant microbial genera identified during Daqu fermentation include <i>Weissella</i>, <i>Lactobacillus</i>, <i>Leuconostoc</i>, <i>Thermoascus</i>, <i>Aspergillus</i>, and <i>Wickerhamomyces</i>. Furthermore, <i>Kroppenstedtia</i>, <i>Saccharopolyspora</i>, <i>Thermoactinomyces</i>, <i>Thermoascus</i>, <i>Aspergillus, Rhizomucor</i>, and <i>Thermomyces</i> emerged as key microorganisms influencing the aroma profile of Daqu. These findings provide a theoretical foundation for quality optimization and standardized production of strong-flavor Daqu.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2025 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/9279456","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144118106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physicochemical Properties and In Vitro and In Vivo Digestive Properties of Pea Starch Complexes Incorporated Different Polyphenols","authors":"Zhaomin Li,&nbsp;Jinjin Xing,&nbsp;Xiaoli Gao,&nbsp;Cairenzhuoma Ma,&nbsp;Wenhua Liao","doi":"10.1155/jfbc/1424706","DOIUrl":"https://doi.org/10.1155/jfbc/1424706","url":null,"abstract":"<div>\u0000 <p>The prevalence of Type 2 diabetes mellitus (T2DM) has become a major public health concern worldwide. Slowly digested or indigestible carbohydrates, such as starch complexes, are associated with a low glycemic index (GI) and a reduced risk of developing T2DM. In this study, pea starch–polyphenol complexes were prepared by incorporating three types of polyphenols with varying polarities: anthocyanin, gallic acid, and quercetin. The physicochemical and digestive properties of these three different pea starch–polyphenol complexes were investigated. The results showed that all three polyphenols could bind with pea starch to form complexes and increase the content of resistant starch. Among them, quercetin resulted in the highest resistant starch content (17.62%), surpassing the other two polyphenols. X-ray diffraction (XRD) analysis indicated that all pea starch–polyphenol complexes exhibited a V-type crystalline structure. The crystallinity of the quercetin–pea starch complex was 5.7%, suggesting a greater formation of amylose–quercetin single helices compared to the other two starch–polyphenol complexes. In vitro digestion results demonstrated that the quercetin–pea starch complex had the highest indigestibility among the three, with α-amylase inhibition ratios of 52.40%, 50.13%, and 49.23% for the quercetin–pea starch, anthocyanin–pea starch, and gallic acid–pea starch complexes, respectively. Consistent with the in vitro findings, in vivo experiments showed that zebrafish fed with the quercetin–pea starch complex exhibited the lowest postprandial glycemic response. Additionally, the intake of the quercetin–pea starch complex led to a lower abundance of harmful gut bacteria, including Proteobacteria and Firmicutes compared to the anthocyanin–pea starch and gallic acid–pea starch complexes. These findings suggest that polyphenols can delay the metabolism of pea starch by inhibiting <i>α</i>-amylase activity and modulating gut microbiota composition. Furthermore, this study highlights that hydrophobic polyphenols exert a more significant impact on the digestibility of pea starch. In summary, quercetin–pea starch complex has great potential in regulating postprandial blood glucose in vitro and in vivo.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2025 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/1424706","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144109001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioactive Flavonoids From Tsaoko Fructus: Antioxidant Capacity and Protection on UV-Damaged Cells and Stimulation to Caenorhabditis elegans Antioxidant System","authors":"Rui-Fang Zhong,&nbsp;Xiao-Mei Zhan,&nbsp;Si-Wen Qin,&nbsp;Wei-Hui Lu,&nbsp;Jian-Guo Jiang,&nbsp;Wei Zhu","doi":"10.1155/jfbc/3228327","DOIUrl":"https://doi.org/10.1155/jfbc/3228327","url":null,"abstract":"<div>\u0000 <p>Tsaoko Fructus (TF), the dried ripe fruit of <i>Amomum tsaoko</i> Crevost et Lemarie <i>(A. tsaoko)</i>, a dietary medicine plant, has been demonstrated to have high antioxidant activity. The main objective of this research was to investigate the isolation and purification of TF flavonoids ([TFFT, TFFC, and TFFP], TFFs) and their chemical constituents and antioxidant effects by in vitro and in vivo experiments. LC-MS/MS was used to clarify the chemical substance basis. The results of antioxidant activity in vitro showed that TFFs had excellent DPPH and ABTS radical scavenging capacity, FRAP, and total reducing power. UVB-damaged HaCaT cells and <i>C. elegans</i> models were used to study the effect of TFFs on oxidative stress. Results revealed that TFFS could significantly postpone oxidative damage in UVB-induced HaCaT cells by suppressing the ROS, MDA, TNF-α, and MMP-1 production and increasing the activity of SOD, CAT, and GSH-Px. Furthermore, TFFs could ameliorate heat resistance and oxidative damage of <i>C. elegans</i>. qRT-PCR results showed that TFFs could upregulate the mRNA expressions of g<i>st-10</i>, <i>sod-3</i>, and <i>ctl-1</i> in <i>C. elegans</i>, indicating TFFs can promote the stress resistance of <i>C. elegans</i> by improving the capacity of the antioxidant oxidase system. Overall, these findings suggested that TFFs may be used as an antioxidant to postpone oxidative stress conditions.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2025 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/3228327","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144100928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Physicochemical Properties, Sugar Contents, and Sensory Attributes of Functional Beverage Developed From Sugar Cane, Moringa, and Barley Malt","authors":"Tariku Zena,&nbsp;Engeda Dessalegn,&nbsp;Welday Hailu","doi":"10.1155/jfbc/1817876","DOIUrl":"https://doi.org/10.1155/jfbc/1817876","url":null,"abstract":"<div>\u0000 <p><i>Moringa</i><i>stenopetala</i> and sugar cane <i>(Saccharum officinarum)</i> are rich sources of nutrients and medicinally important phytochemicals. In this research, functional beverage products were developed from <i>Moringa stenopetala</i> leaf, sugar cane, and barley malt. Optimum conditions were determined using a mixture design. The American Society of Brewing Chemists’ quality control parameters and soft drink quality parameters were employed to evaluate the physicochemical properties. Sugar contents were analyzed using the Lane and Eynon method; sensory attributes were evaluated with the descriptive method using a hedonic scale. Results of analysis indicated that as <i>Moringa stenopetala</i> proportion increased, titratable acidity and percent of ash increased, whereas pH, total soluble solids, and percent of alcohol decreased. Sugar content was increased with an increment of sugar cane proportion and malting, which hydrolyzed and liquefied starch. Maillard and caramelization reactions that took place during processing enhanced the physicochemical properties and sensory attributes of the products. The overall sensory acceptability score ranged from 3.81 ± 0.02 to 4.21 ± 0.05. CM5 and CM7 exhibited better sensory acceptance, and CM8 and CM9 exhibited better physicochemical properties compared with the other developed products.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2025 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/1817876","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144085313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-Inflammatory Effects of Immature Pomelo Fruit Polysaccharides on Experimental Ulcerative Colitis Via Modulation of Gut Microbiota and the ERK1/2 Signaling Pathway","authors":"Zuoliang Zheng,&nbsp;Keman Yin,&nbsp;Ming Xie,&nbsp;Zhiwei Liu,&nbsp;Yuping Zhong","doi":"10.1155/jfbc/5524965","DOIUrl":"https://doi.org/10.1155/jfbc/5524965","url":null,"abstract":"<div>\u0000 <p>The immature pomelo fruit (IPF) of Citrus maxima (Burm.) Merr. is an agricultural by-product rich in bioactive compounds, particularly the lipophilic flavonoid naringin. However, research on water-soluble polysaccharides, another critical component of IPF, including their fundamental structural characteristics and biological activities, remains limited. In this study, a crude polysaccharide named immature pomelo fruit polysaccharides (IPFP), with a polysaccharide content of 81.10%, was extracted from the IPF using the water extraction-alcohol precipitation method. High-performance gel permeation chromatography with refractive index detection (HPGPC-RID) revealed that IPFP consists of two components: IPFP-1 with a molecular weight of 9187 Da and IPFP-2 with a molecular weight exceeding 100 kDa. Analysis via 1-phenyl-3-methyl-5-pyrazolone-high performance liquid chromatography (PMP-HPLC) indicated that IPFP primarily comprises galactose, glucose, and xylose, with glucose being the predominant monosaccharide. To investigate the biological activity of IPFP, a mouse model of acute ulcerative colitis was established using 2.5% dextran sulfate sodium (DSS). Fifty BALB/c mice were randomly assigned to five groups: a control group, a DSS-induced model group, and three IPFP treatment groups with low, medium, and high doses. Molecular biological techniques, including quantitative polymerase chain reaction (qPCR), Western blotting, and 16S ribosomal RNA (16S rRNA) sequencing, were employed to evaluate the expression of inflammatory genes in colon tissues, activation of the extracellular signal-regulated kinase (ERK) signaling pathway, and composition of fecal microbiota. The animal experiments demonstrated that IPFP significantly alleviated DSS-induced symptoms, such as body weight loss and colon shortening. Additionally, it mitigated colonic histopathological damage, suppressed the expression of proinflammatory cytokines tumor necrosis factor-alpha (TNF-<i>α</i>), interleukin-1 beta (IL-1β), and interleukin-6 (IL-6), while promoting the expression of the anti-inflammatory cytokine interleukin-10 (IL-10). Notably, the high-dose IPFP group exhibited the most pronounced effects. Following IPFP treatment, there was a significant reduction in the phosphorylation levels of ERK1/2 protein and restoration of intestinal microbiota diversity and abundance. These findings suggest that IPFP may alleviate DSS-induced inflammatory damage by modulating gut microbiota, particularly bacteroidetes, and inhibiting the ERK1/2 pathway. Our study provides initial insights into the structure and pharmacological mechanisms of IPFP against ulcerative colitis, highlighting its potential as an anti-inflammatory agent for promoting intestinal health in inflammatory diseases.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2025 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/5524965","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144074518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic Effects of the Combination of Quercetin, Naringin, and Rutin on Cervical Cancer: Roles of Apoptosis and Autophagy","authors":"Sara Goharpour,&nbsp;Mostafa Rahimnejad,&nbsp;Ghasem Najafpour,&nbsp;Mohammad Hosein Farzaei","doi":"10.1155/jfbc/9402123","DOIUrl":"https://doi.org/10.1155/jfbc/9402123","url":null,"abstract":"<div>\u0000 <p>Cervical cancer is, to date, the second most common cause of cancer in female patients worldwide. Most of the present-day research activities have concentrated on studies of antitumor medicinal plants and bioactive compounds obtained from natural flavonoids such as quercetin (Q), naringin (N), and rutin (R). The present study reported an in vitro method for assessing the combined anticancer activity of quercetin, naringin, and rutin in combination in HeLa cervical cancer cells. The anticancer potential of the compounds was investigated via experiments involving apoptosis, detection of ROS levels, rhodamine 123 staining, AO/EtBr staining, scratch tests, real-time methods, and Western blot techniques in protein-mediated autophagy pathways. On the basis of the results from the MTT assay and combination index, reasonable combinations of RQ and RQN were selected. Experiments revealed significant upregulation of Beclin1 protein levels and corresponding downregulation of P62, indicating enhanced autophagic flux in HeLa cells treated with flavonoid combinations. Assays for apoptosis revealed that the total number of apoptotic cells increased in a time-dependent manner, and the percentages of apoptotic cells enriched with the RQN combination were 16.07%, 65.02%, and 8.91% at 24, 48, and 72 h, respectively. The combination of RQ resulted in the following percentages of apoptotic cells at the same time points: 31.04%, 32.36%, and 11.46%. Real-time PCR and Western blotting of autophagy-related marker proteins revealed that the flavonoid combinations drastically modulated the autophagy pathway in HeLa cells by increasing the expression levels of Beclin1 (protein and expression levels) and <i>LC3</i> (expression), whereas the expression level of P62 (protein and expression levels) notably decreased. Thus, these data suggest that flavonoid combinations may induce cervical cancer cell death through an autophagy-mediated mechanism and apoptosis. These flavonoid combinations represent potential therapeutic strategies for treating cervical cancer.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2025 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/9402123","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144074700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}