Journal of Food Biochemistry最新文献

{"title":"Rapidly and Efficiently Screening and Identification of Xanthine Oxidase Inhibitory Peptides From Protein Hydrolysate of Antarctic Krill","authors":"Xue Liu,&nbsp;Wenya Meng,&nbsp;Jing Zhang,&nbsp;Ye Wang,&nbsp;Long Xing,&nbsp;Huiyang Yu,&nbsp;Hu Hou,&nbsp;Ping Dong","doi":"10.1155/jfbc/9735450","DOIUrl":"https://doi.org/10.1155/jfbc/9735450","url":null,"abstract":"<div>\u0000 <p>With increasing public awareness of gout and hyperuricemia, the quest for xanthine oxidase (XOD) inhibitors to alleviate elevated uric acid levels has emerged as a central approach in hyperuricemia treatment. Defatted Antarctic krill meal, a cost-effective byproduct of krill oil production, is enriched with high-quality protein. This study aimed to evaluate the inhibition of the peptides from protein hydrolysate of Antarctic krill against XOD. Alcalase was implemented for the enzymatic preparation of proteins, and the optimal hydrolysis conditions were optimised. Utilising affinity ultrafiltration and molecular docking techniques, innovative XOD inhibitory peptides were isolated and identified from the protein hydrolysate of defatted Antarctic krill meal (AKAP). The inhibitory activities of these peptides were validated in vitro. A total of 943 peptides were identified by LC-MS/MS analysis, and their binding affinity to XOD was assessed using molecular docking. Among the peptides screened, Leu-Pro-Pro-Tyr-Ser-Lys-Glu (LPPYSKE, 832.43 Da, IC₅₀ = 0.332 ± 0.05 mg/mL) exhibited the highest XOD inhibitory activity. Molecular docking results revealed that salt bridges, hydrocarbon bonding and π–alkyl groups significantly affected the interaction between XOD inhibitory peptides and key residues, namely, GLU802, PHE914 and PHE1009. Overall, these results underscore the potential of AKAP as a promising candidate for the development of functional foods against hyperuricemia.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2025 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/9735450","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143762232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long-Term Pretreatment With D-Limonene Protects Gastric Lipid Composition by Reducing Oxidative Damage and Inflammation in Indomethacin-Induced Gastric Injury","authors":"Mehmet Alkanat,&nbsp;Hafize Özdemir Alkanat","doi":"10.1155/jfbc/6639397","DOIUrl":"https://doi.org/10.1155/jfbc/6639397","url":null,"abstract":"<div>\u0000 <p>D-Limonene is a monoterpene compound that is widely contained in citrus plants. It has been demonstrated to have several beneficial properties. The present study aimed to investigate the effect of D-limonene against indomethacin-induced gastric ulceration. D-Limonene was administered orally for 21 days. Gastric ulceration was induced by oral administration of 25 mg/kg indomethacin via gastric gavage, five minutes after the administration of Lim or ranitidine. The stomachs of the rats were examined macroscopically and histopathologically to assess gastric lesions. GR, MDA, caspase-3, TNF-α, PGE₂ levels, and iNOS, catalase, and SOD activities were measured using the ELISA method. The lipid composition of gastric tissue was measured using a high-performance thin-layer chromatography method. The alteration observed in the lipid profile of the indomethacin group differed from that of the control group. In contrast, the lipid profile of the Lim-treated groups was similar to that of the control group. Although the L50 group demonstrated a significant difference compared to the Ind group regarding GR, catalase, caspase-3, and iNOS activity, no significant differences were observed in MDA, SOD, TNF-α, and PGE₂ levels when compared to the Ind group. However, no indomethacin-induced gastric damage was observed in the macroscopic and microscopic evaluations of gastric tissues collected from the L100 and L250 groups. Additionally, the gastroprotective activity of these two groups was noted in all tests. This study’s results indicated that a 50 mg/kg dose of D-limonene was subeffective, while 100 and 250 mg/kg doses showed gastroprotective activity in rats.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2025 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/6639397","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143761832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ameliorative Effect of Glycerol Monolaurate on Glucose Dyshomeostasis in db/db Mice Associated With the PGC-1α Signaling Pathway and Intestinal Microbiota","authors":"Haiying Cai,&nbsp;Menghui Lin,&nbsp;Li Chen,&nbsp;Minjie Zhao,&nbsp;Jing Wang,&nbsp;Fengqin Feng,&nbsp;Junhui Zhang","doi":"10.1155/jfbc/8887109","DOIUrl":"https://doi.org/10.1155/jfbc/8887109","url":null,"abstract":"<div>\u0000 <p>Glycerol monolaurate (GML) has previously been demonstrated to improve insulin resistance in mice fed a high-fat diet (HFD), yet whether GML can enhance glucose homeostasis in diabetic mice remains uncertain. In the current study, BKS mice and BKS-db/db mice were fed a normal chow diet and administered GML solution by gavage for 9 weeks. Weekly postprandial blood glucose, water intake, and weight were recorded at regular intervals. Serum metabolic indicators, liver gene expression, and intestinal microbiota were also assessed. The results revealed that GML significantly decreased the postprandial blood glucose in the later stage of the feeding period, markedly reduced water intake in db/db mice, maintained body weight, and significantly improved glucose and insulin tolerance. Moreover, GML significantly reduced fasting blood glucose and insulin resistance index. GML also significantly inhibited the proliferator-activated receptor-γ coactivator-1α (PGC-1α) signaling pathway, thereby suppressing the gluconeogenic pathway, but activated the gene expression of glucokinase in the glycolysis pathway. GML also significantly altered the gut microbiota, decreasing the abundance of Firmicutes, <i>Lactobacillus</i>, and Lachnospiraceae, while increasing the abundance of Bacteroidetes, <i>Helicobacter</i>, <i>Parabacteroides</i>, and S24-7. Our findings suggest that GML modulates gut microbiota and inhibits the PGC-1α signaling pathway, which may be associated with the improved glucose homeostasis observed.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2025 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/8887109","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143735521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic Analysis of the Mechanisms Underlying Delayed Quality Deterioration in Fresh-Cut Asparagus Lettuce Using Ascorbic Acid and L-Cysteine in a Sodium Carboxymethyl Cellulose Coating","authors":"Huibo Song,&nbsp;Yi Wang,&nbsp;Yanan Chen,&nbsp;Wei Wang,&nbsp;Guihong Zhao,&nbsp;Lijuan Yu,&nbsp;Yuxing Guo,&nbsp;Xiaoxue Kong,&nbsp;Haibo Luo","doi":"10.1155/jfbc/8824424","DOIUrl":"https://doi.org/10.1155/jfbc/8824424","url":null,"abstract":"<div>\u0000 <p>Browning and green loss are two typical characteristics of quality deterioration in fresh-cut asparagus lettuce (<i>Lactuca sativa</i> var. angustata). In the present study, the mechanism underlying the delay in quality deterioration of fresh-cut asparagus lettuce by the solution was elucidated through transcriptomic analysis. Fresh-cut asparagus lettuce was immersed in a solution containing 0.67% sodium carboxymethyl cellulose (CMC-Na), 0.85% L-cysteine (L-Cys), 0.38% ascorbic acid (AsA), and the distilled water (control) for 10 min, respectively. Both treated and control samples were stored in the dark at 4°C with 80%–90% relative humidity for eight days. The results showed that treatment with the solution retarded the cut surface browning and green color loss and extended the shelf life of fresh-cut asparagus lettuce. In comparison to a control group, the treatment effectively attenuated the upregulation of the <i>LsACO3</i> and <i>LsACS1</i> genes; it downregulated the expression of the phospholipase-related genes <i>LsNPC6</i>, <i>LsPLC2</i>, and <i>At2g42690</i>. Conversely, there was substantial upregulation of gene expression related to <i>L. sativa</i> calmodulin-like proteins and <i>L. sativa</i> calcium-dependent protein kinases, along with activation of the mitogen-activated protein kinase (MAPK) signaling pathway and significant upregulation of genes associated with the <i>L. sativa</i> jasmonic acid biosynthesis pathway involved in the defense response. In conclusion, our findings indicated that treatment with the solution effectively delays the deterioration of fresh-cut asparagus lettuce during storage by inhibiting endogenous ethylene synthesis, mitigating reactive oxygen damage, and enhancing disease resistance, thereby providing superior preservation of its color and texture. The results provide an alternative method for delaying color deterioration of fresh-cut produce.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2025 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/8824424","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143726845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Green and Rapid High-Performance Liquid Chromatography–Ultraviolet Method for Determination of Four Organic Acids in Different Parts of Dandelion With Blue Applicability Grade Index Assessment","authors":"Jing Chen,&nbsp;Qinggui Lei,&nbsp;Weiqi Yang,&nbsp;Wenhao Wang,&nbsp;Guang’ e Tan,&nbsp;Zhengming Qian","doi":"10.1155/jfbc/4077536","DOIUrl":"https://doi.org/10.1155/jfbc/4077536","url":null,"abstract":"<div>\u0000 <p>Dandelion is a functional food that serves both medicinal and dietary purposes, which requires quality control to ensure its safety and efficacy. This study established a rapid high-performance liquid chromatography-ultraviolet (HPLC-UV) method for analyzing four organic acids in different parts of dandelion. After ultrasonic extraction with 40% methanol, samples were analyzed using an Agilent InfinityLab Poroshell 120 EC-C₁₈ column with a mobile phase of 0.1% formic acid aqueous-methanol and detection wavelength at 325 nm. The method completed the analysis in 5 min (2 min for extraction and 3 min for chromatographic analysis). The 4 organic acid standards (caftaric acid, chlorogenic acid, caffeic acid, and chicoric acid) showed excellent linearity (<i>r</i> &gt; 0.9995), and the average spiked recoveries were 101.38%, 96.85%, 103.94%, and 100.15% (relative standard deviations were 1.00%, 1.00%, 0.97%, and 0.55%), respectively. The total content of 4 organic acids is highest in dandelion flowers (1.355 mg/g dry weight [DW]), followed by stems/leaves (1.182 mg/g DW), and lowest in roots (0.026 mg/g DW). Additionally, to evaluate the environmental impact of both new and reported methods, this study performed an analysis using the blue applicability grade index. The developed methodology obtained the highest score of 80.0, which indicated that the developed HPLC method was most effective and eco-friendly. In conclusion, this method is rapid, accurate, and eco-friendly, which is help to the product development and quality evaluation of dandelion.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2025 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/4077536","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143707441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Apoptotic and Cytotoxic Potential of Carnosic Acid- and Carnosol-Rich Fractions From Salvia dorystaechas on Human Hepatoma (HepG2) Cells","authors":"İlker Çinbilgel,&nbsp;Naciye Erkan,&nbsp;Serap Kocaoğlu Cenkci,&nbsp;Timur Tongur,&nbsp;Taner Erkaymaz,&nbsp;Kudret Akpınar","doi":"10.1155/jfbc/4440039","DOIUrl":"https://doi.org/10.1155/jfbc/4440039","url":null,"abstract":"<div>\u0000 <p>Plant isolates from <i>Salvia dorystaechas</i> (Lamiaceae) were investigated for their apoptotic and cytotoxic effects on human hepatocellular carcinoma (HepG2) cells under various treatment conditions. Chromatographic isolation yielded fractions (Fr C, D, F, and H) rich in carnosic acid (CA) and carnosol (CL), with significantly higher concentrations (26.3–93.5 g/kg) compared to the crude extract (10.0 g/kg). Other phenolic compounds, including caftaric acid, epicatechin, quercetin, and rutin, were also detected in notable amounts via ultra-high-performance liquid chromatography-mass spectrometry/mass spectrometry (UHPLC-MS/MS). CA-/CL-rich fractions effectively induced caspase-9 and caspase-3 activities, decreased cell viability, and increased lactate dehydrogenase (LDH) release. These effects were significant compared to those induced by 0.1 mM hydrogen peroxide (H₂O₂). Notably, Fr D and Fr H, which contained the highest CA/CL concentrations, showed caspase-9/caspase-3 activities of 1.60/1.83 and 1.63/1.94, respectively, at 25 μg/mL after 24 h of treatment, whereas the crude plant extract showed corresponding activity values of 1.33/1.36 under the same conditions. In contrast, the crude extract exhibited its strongest apoptotic and cytotoxic effects under harsher conditions (50 μg/mL and 48 h), with caspase-9/caspase-3 activities of 1.70/2.59, 12.0% cell viability, and 65.4% LDH release. Apoptosis was further supported by superoxide (O₂•⁻) production mediated by NADPH oxidases (NOXs), as the NOX inhibitor diphenyleneiodonium (DPI) reduced O₂•⁻ levels by 28.6%–48.6%. Additionally, nuclei-stained cells revealed morphological changes, including nuclear fragmentation, apoptotic body formation, and loss of adhesion. In conclusion, CA-/CL-rich <i>S. dorystaechas</i> isolates demonstrated strong apoptotic and cytotoxic effects on HepG2 cells, with contributions from other phenolic compounds. These findings highlight the potential therapeutic value of <i>S. dorystaechas</i> isolates in liver cancer treatment.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2025 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/4440039","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143707442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effects of Bitter Melon (Mormordica charantia) on Weight Loss and Body Composition: A Systematic Review and Dose-Response Meta-Analysis of Randomized Controlled Trials","authors":"Mohammad Reza Amini,&nbsp;Sanaz Pourreza,&nbsp;Camellia Akhgarjand,&nbsp;Fatemeh Sheikhhossein,&nbsp;Gholamreza Askari,&nbsp;Azita Hekmatdoost","doi":"10.1155/jfbc/2921435","DOIUrl":"https://doi.org/10.1155/jfbc/2921435","url":null,"abstract":"<div>\u0000 <p>Results from the clinical research about <i>Momordica charantia</i> (MC) benefits on weight loss have been contradictory. This study, which included the most available randomized placebo-controlled trials (RCTs), was the first to investigate the impact of MC supplementation on body weight and composition. The effects of the given MC on weight, body mass index (BMI), waist circumference (WC), and percentage of body fat (PBF) were examined in RCTs up to February 2023 (without limitation on release date). Online databases (PubMed/Medline, Google Scholar, Scopus, and Web of Science) were searched for these studies. The inclusion and exclusion criteria were followed in the screening and evaluation of the literature. The Cochrane tool was used to assess bias risk. There were a total of ten RCTs, including 448 participants. The meta-analysis revealed that MC supplementation had no effect on weight (weighted mean difference (WMD): 0.04 kg; 95% confidence interval ([CI]: −0.16 to 0.25; <i>p</i> = 0.66), BMI (WMD: −0.18 kg/m²; 95% CI: −0.43 to 0.25; <i>p</i> = 0.16), WC (WMD: −0.94 cm; 95% CI: −3.04 to 1.16; <i>p</i> = 0.37), and BFP (WMD: −0.99; 95% CI: −2.33 to 0.35; <i>p</i> = 0.14). According to the subgroup analysis, MC could lower the BMI at a dosage of ≤ 2000 mg/d when compared to a placebo group (<i>p</i>&lt;0.001). The dose-response analysis represented a significant nonlinear connection between the MC dosage and weight reduction (<i>P</i>_{non−linearity} = 0.011); the effect on weight loss was observed the most at doses of 2000 and 4500 mg. Moreover, MC consumption decreased the BMI by the sixth week and then increased the BMI up to the 17th week (<i>P</i>_nonlinearity = 0.03). Our results indicate that MC consumption can reduce the BMI in some dosages and duration of supplementation.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2025 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/2921435","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143707705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study on Bacterial Community Changes and Volatile Flavor Correlation During Fermentation of Low-Salt Dry-Cured Ham","authors":"Yijia Sun,&nbsp;Xin Liu,&nbsp;Jingqi Cao,&nbsp;Yingdao Nan,&nbsp;Baide Mu,&nbsp;Guanhao Li,&nbsp;Tingyu Li,&nbsp;Chunxiang Piao","doi":"10.1155/jfbc/9709367","DOIUrl":"https://doi.org/10.1155/jfbc/9709367","url":null,"abstract":"<div>\u0000 <p>The aim of this work is to elucidate the effects of reduced salt content on the microbial ecology and flavor profile of dry-cured ham. To develop the low-salt (LS) treatment group, a curing mixture consisting of 18% potassium lactate, 12% lysine, and 70% sodium chloride was utilized instead of traditional curing salts. On the other hand, the standard protocol for curing the control group (CK) involved the use of 100% sodium chloride. The results demonstrate that the ham in the LS group had a noteworthy 9.01% increase in protein content when compared to the ham in the CK group. Through SPME-GC-MS analysis, 40 distinct types of volatile compounds were identified. The LS group had a much higher nonanal content, which improved the ham’s flavor and rose aroma. Illumina Miseq sequencing indicates that the genus <i>Staphylococcus</i> is the most common microbial genus at the late ripening stage of dry-cured ham. Spearman correlation analysis revealed that <i>Staphylococcus</i> had a significant relationship with the C18:0 concentration and was substantially linked with most metabolites. Finally, instead of utilizing the standard 30% sodium chloride, it is recommended to process dry-cured ham using a formulation that comprises 18% potassium lactate, 12% lysine, and 70% sodium chloride. It is anticipated that this substitution will result in a higher production of volatile compounds and <i>Staphylococcus</i>, which will enhance the overall quality and flavor profile of the finished product.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2025 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/9709367","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143698903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling the Phenolic Profiling of Moroccan Ferula communis L. Fruits: A Combination of In Silico and In Vivo Protective Effect Against Methotrexate-Induced Hepato-Renal Dysfunction","authors":"Ghizlane Nouioura,&nbsp;Mohamed El Fadili,&nbsp;Mohamed Bouhrim,&nbsp;El Hassania Loukili,&nbsp;Hazem K. Ghneim,&nbsp;Mourad A. M. Aboul-Soud,&nbsp;John P. Giesy,&nbsp;Badiaa Lyoussi,&nbsp;Elhoussine Derwich","doi":"10.1155/jfbc/1106215","DOIUrl":"https://doi.org/10.1155/jfbc/1106215","url":null,"abstract":"<div>\u0000 <p><b>Background:</b> Methotrexate (MTX) is associated with several side effects, including hepatic and renal toxicities, which limit its effectiveness as an anticancer medication. These toxicities can lead to hepatotoxicity, nephrotoxicity, and potential liver and kidney failure. On the other hand, the fruit of the giant fennel plant (<i>Ferula communis</i>) contains bioactive compounds with proven antioxidant and anti-inflammatory properties, as demonstrated in previous studies. These compounds have protective effects against various diseases.</p>\u0000 <p><b>Aim of the Study:</b> The current study combines <i>in silico</i> ADMET prediction and <i>in vivo</i> evaluation of hepatorenal toxicity of MTX in rats to predict potential effects in humans.</p>\u0000 <p><b>Materials:</b> Chemical constituents of FC were identified using HPLC-DAD analysis. <i>In silico</i> pharmacokinetics, ADMET predictions, and Egan’s boiled egg model were employed. For the <i>in vivo</i> experiments, 32 rats were divided into four groups to investigate the impact of FC extract (FC-Ext) on MTX-induced hepatorenal toxicity. The rats were pretreated with FC-Ext (250 mg/kg, body mass, dissolved in distilled water) from days 1 to 21 and administered MTX (25 mg/kg) on day 7. Various parameters, including body and organ masses, serum toxicity biomarkers (such as albumin, ALT, AST, BUN, creatinine, uric acid, and total protein), hematological parameters, and tissue histopathology, were evaluated to assess MTX-induced hepato-renal damage.</p>\u0000 <p><b>Results:</b> The analysis of FC revealed the presence of various phenolic acids, with 15 compounds identified using HPLC-DAD. ADMET prediction provided assurance regarding the safety of the phytochemical compounds from FC concerning liver injury. Experimental validation <i>in vivo</i> confirmed that MTX-induced hepato-renal damage led to increased levels of AST, ALT, BUN, and creatinine in blood serum. Hemoglobin, total protein, albumin, and uric acid levels decreased following exposure to MTX. However, co-treatment with a hydro-ethanolic extract of FC significantly mitigated these changes, restored kidney and liver functions, and preserved tissue architecture.</p>\u0000 <p><b>Conclusion:</b> FC-Ext shows promise in managing liver and renal diseases and mitigating the hematological toxicity induced by MTX. These findings suggest potential applications for humans in preventing MTX-induced renal, hepatic, and hematological toxicity.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2025 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/1106215","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143689100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hepatoprotective Effects of Fermented Laminaria japonica in Oleic Acid–Induced HepG2 Cells and in a High-Fat Diet–Induced NAFLD Mice Model via Activating the SIRT1 Signaling Pathway","authors":"Qiulin Yue,&nbsp;Xianwei Wang,&nbsp;Yongxuan Liu,&nbsp;Mengrui Cai,&nbsp;Shousen Guo,&nbsp;Lin Zhao,&nbsp;Le Su,&nbsp;Song Zhang,&nbsp;Baojun Li,&nbsp;Chen Zhao,&nbsp;Kunlun Li","doi":"10.1155/jfbc/1706266","DOIUrl":"https://doi.org/10.1155/jfbc/1706266","url":null,"abstract":"<div>\u0000 <p><i>Laminaria japonica</i> is a kind of brown algal with good nutritional and medicinal value. Our previous studies demonstrated that the phytochemical components and bioactivities of <i>Laminaria japonica</i> were markedly enhanced by fermentation. However, the specific activity and underlying mechanism of fermented <i>Laminaria japonica</i> (FLJ) against NAFLD remain poorly understood. For this study, <i>Laminaria japonica</i> was fermented using <i>S. cerevisiae</i> and <i>Lactiplantibacillus</i>. <i>In vitro</i> assays were employed to evaluate the efficacy of FLJ in regulating cholesterol reduction and fat binding. In addition, the hepatoprotective effects and underlying mechanisms of FLJ in the treatment of NAFLD were evaluated using oleic acid (OA)–induced HepG2 cells and NAFLD mice. Our data revealed that FLJ significantly promoted fat-binding and cholesterol-reducing activity <i>in vitro</i>. Moreover, FLJ alleviated hepatic lipid accumulation, oxidative stress, and the inflammatory response in high-fat diet (HFD)–induced NAFLD mice and in OA–induced HepG2 cells. The underlying protective mechanisms of FLJ against NAFLD may be attributed to the activation of the SIRT1 signaling pathway. These observations suggested that FLJ could be developed as a functional food supplement for the prevention or improvement of NAFLD.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2025 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/1706266","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143638659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}