Journal of Food Biochemistry最新文献

{"title":"Harnessing Triticale (× Triticosecale Wittmack) Malting for Amylase Enzyme Synthesis and Partial Purification","authors":"Diego Girón-Orozco,&nbsp;Erick Heredia-Olea,&nbsp;María Dolores Mariezcurrena-Berasain,&nbsp;Oscar Alejandro Aguilar-Jiménez,&nbsp;Amaury-M. Arzate-Fernández","doi":"10.1155/jfbc/6002195","DOIUrl":"https://doi.org/10.1155/jfbc/6002195","url":null,"abstract":"<p>Triticale malt is an underutilized cereal matrix with high amylolytic potential, which may be exploited for the recovery of plant-derived enzymes for food and biotechnological applications. This study aimed to obtain α-amylase- and β-amylase-enriched aqueous extracts from Bicentenario and Siglo XXI triticale malts and to partially purify these enzymes using ethanol/trisodium citrate aqueous two-phase systems (ATPS). Triticale grains were malted by germination for 5 days and dried at 50°C. Aqueous extraction was performed using ground malt and deionized water at a 1:10 ratio under different extraction times (30, 120, and 270 min) and temperatures (30°C and 40°C). The selected extracts were lyophilized and subjected to ATPS composed of ethanol and trisodium citrate. Enzyme activity was determined using the BETAMYL-3 method for β-amylase and the AOAC 2002.01, AACC 22-02.01, and ICC Standard No. 303 methods for α-amylase. Mild extraction conditions favored enzyme recovery, with the highest α-amylase activity observed in Siglo XXI extract at 30 min/30°C, increasing from 99.10 CU/g in malt to 507.42 CU/g in the aqueous extract. β-Amylase activity was also higher in Siglo XXI than in Bicentenario extracts. The 30% ethanol/18% trisodium citrate ATPS was the most effective system, promoting differential partitioning with α-amylase concentrated in the bottom phase (432.89 CU/mL) and β-amylase in the top phase (5.78 BU/mL). These results highlight the potential of triticale malt as a renewable source of amylolytic enzymes and support ethanol/trisodium citrate ATPS as a mild, sustainable strategy for obtaining amylase-enriched fractions, although further optimization is needed to improve β-amylase recovery and scalability.</p>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2026 1","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/6002195","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148942409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ampelopsis grossedentata (Vine Tea) Ethanol Extract Protects Against Oxidative Damage and Aging in Mice Via Nrf2 Activation","authors":"Wei Weng,&nbsp;Yingfei Yang,&nbsp;Liangyu Shi,&nbsp;Kailing Pan,&nbsp;Haiyan Wang","doi":"10.1155/jfbc/4093573","DOIUrl":"https://doi.org/10.1155/jfbc/4093573","url":null,"abstract":"<p><i>Ampelopsis grossedentata</i>, a traditional food–medicinal vine rich in flavonoids, was investigated here for its antiaging potential and underlying mechanisms. An ethanol extract (AGE) was chemically profiled by UHPLC–ESI–HRMS/MS (16 compounds identified; dihydromyricetin ≈192.7 mg g⁻¹) and shown to contain high levels of phenolics and flavonoids (TPC 348.4 mg GAE·g⁻¹; TFC 213.4 mg RE·g⁻¹) with potent in vitro antioxidant activity (DPPH IC₅₀ 34.3 ± 1.8 μg mL⁻¹; ABTS IC₅₀ 40.3 ± 4.8 μg mL⁻¹). AGE protected SH-SY5Y cells from H₂O₂-induced injury by reducing intracellular ROS, preserving viability, and decreasing apoptosis. In a D-galactose–induced mouse aging model, oral AGE ameliorated behavioral and hippocampal histopathological changes, restored organ indices, increased serum antioxidant defenses (T-AOC, SOD, CAT, and GSH-Px), and reduced lipid peroxidation (MDA). Mechanistically, AGE upregulated Nrf2 and downstream effectors NQO1 and HO-1 and reversed proapoptotic and proinflammatory gene changes (improved Bcl-2/Bax ratio; decreased caspase-3, p53, p65, and iNOS). Molecular docking supported potential interactions between AGE flavonoids and key antioxidant/antiapoptotic targets. Collectively, these findings indicate that AGE attenuates oxidative stress, inflammation, and apoptosis via activation of Nrf2 signaling and related pathways, supporting its promise as a food-based candidate for prevention or mitigation of age-related oxidative damage.</p>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2026 1","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/4093573","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148942408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quercitrin and Quercetin From Piliostigma malabaricum and Cheniella lakhonensis: Ultrasound-Assisted Extraction, HPLC and LC-MS Analysis, and Bioactivity Evaluation","authors":"Wannachai Chatan,&nbsp;Pakin Noppawan,&nbsp;Jantima Upan,&nbsp;Wanwisa Lijuan,&nbsp;Wilawan Promprom","doi":"10.1155/jfbc/7906493","DOIUrl":"https://doi.org/10.1155/jfbc/7906493","url":null,"abstract":"<p>This study investigated the phytochemical composition, antioxidant capacity, and anti-inflammatory potential of ethanolic leaf extracts of <i>Piliostigma malabaricum</i> and <i>Cheniella lakhonensis</i>. Preliminary phytochemical screening showed that both species contained phenolic compounds, flavonoids, tannins, saponins, and cardiac glycosides, indicating diverse secondary metabolites. Quantitative analysis showed that <i>C. lakhonensis</i> contained higher total phenolic, flavonoid, and tannin contents than <i>P. malabaricum</i>. The antioxidant activity of the extracts was assessed using the DPPH radical-scavenging assay and was significantly stronger in <i>C. lakhonensis</i> (IC₅₀ = 132.77 ± 3.80 μg/mL) than in <i>P. malabaricum</i> (IC₅₀ = 322.96 ± 2.77 μg/mL), compared with ascorbic acid (IC₅₀ = 68.25 ± 2.30 μg/mL). Both extracts demonstrated significant suppression of albumin denaturation under the evaluated in vitro conditions, indicating initial anti-inflammatory capabilities, with <i>P. malabaricum</i> showing a slightly higher inhibitory effect. HPLC quantification and LC-MS analysis investigations verified the existence of quercetin and quercitrin in both species, with elevated concentrations observed in <i>C</i>. <i>lakhonensis</i>. These results offer initial in vitro evidence suggesting that both species could be valuable sources of bioactive compounds; nonetheless, further antioxidant assays, mechanistic research, safety assessments, and in vivo studies are necessary prior to confirming their functional food or therapeutic uses.</p>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2026 1","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/7906493","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148942121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Geniposide Ameliorates Hepatic Steatosis in MASLD Mice via Modulation of the CYP4A/20-HETE Axis","authors":"Ruixi Luo,&nbsp;Yang Yang,&nbsp;Xiaoqian Yang,&nbsp;Siman Huang,&nbsp;Qihang Sun,&nbsp;La Wang,&nbsp;Wenjia Wang,&nbsp;Ping Wang,&nbsp;Weiyi Tian,&nbsp;Peng Chen","doi":"10.1155/jfbc/3170493","DOIUrl":"https://doi.org/10.1155/jfbc/3170493","url":null,"abstract":"&lt;div&gt;\u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Background&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;Metabolic dysfunction–associated steatotic liver disease (MASLD) is a highly prevalent chronic liver disorder for which effective pharmacological interventions remain limited, particularly at early stages. Geniposide (GP), a major bioactive component derived from the fruit of &lt;i&gt;Gardenia jasminoides&lt;/i&gt;, has been reported to exert hepatoprotective, anti-inflammatory, and antioxidant effects; however, its molecular mechanisms in MASLD have not been fully elucidated.&lt;/p&gt;\u0000 &lt;/section&gt;\u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Methods&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;An MASLD mouse model was established by feeding ApoE knockout mice a high-fat diet (HFD), followed by GP treatment for 8 weeks. Hepatic steatosis, lipid metabolism, oxidative stress, and inflammatory responses were evaluated using biochemical assays, histopathological analysis, and immunofluorescence. Unbiased Astral data-independent acquisition (Astral-DIA) proteomics was performed to identify differentially expressed proteins and enriched signaling pathways. &lt;i&gt;In vitro&lt;/i&gt; validation was conducted using palmitic acid (PA)–treated THLE-2 human hepatocytes.&lt;/p&gt;\u0000 &lt;/section&gt;\u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Results&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;GP treatment significantly alleviated hepatic steatosis and lipid metabolic disorder in HFD-fed mice. Compared with the HFD group, GP reduced serum TG levels from 1.38 ± 0.15 to 0.79 ± 0.10 mM (&lt;i&gt;p&lt;/i&gt; &lt; 0.05) and hepatic TG content from 3.15 ± 0.47 to 2.21 ± 0.28 mmol/g protein (&lt;i&gt;p&lt;/i&gt; &lt; 0.05). Proteomic analysis identified more than 7600 proteins and revealed CYP4A family members as prominent GP-responsive proteins, with Cyp4a10 and Cyp4a14 reduced by approximately 62% and 71%, respectively. Consistently, GP decreased hepatic 20-HETE levels from 114.6 ± 28.52 to 80.63 ± 19.12 ng/mg protein (&lt;i&gt;p&lt;/i&gt; &lt; 0.05) and reduced oxidative and inflammatory responses, including decreased serum IL-6 from 127.3 ± 42.27 to 77.67 ± 23.55 pg/mL and TNF-α from 44.18 ± 10.47 to 28.58 ± 8.53 pg/mL (both &lt;i&gt;p&lt;/i&gt; &lt; 0.05). In vitro, GP restored PA-reduced THLE-2 cell viability from 69.0 ± 7.0% to 88.0 ± 8.0% of the control level (&lt;i&gt;p&lt;/i&gt; &lt; 0.05) and decreased intracellular 20-HETE levels from 60.77 ± 9.06 to 40.28 ± 7.82 ng/mg protein (&lt;i&gt;p&lt;/i&gt; &lt; 0.05). GP also attenuated PA-induced lipid accumulation, ROS generation, inflammatory cytokine secretion, and apoptosis. Pharmacological inhibition of 20-HETE synthesis partially recapitulated these protective effects, supporting the involvement of the CYP4A/20-HETE axis in hepatocyte lipotoxicity.&lt;/p&gt;\u0000 &lt;/section&gt;\u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Conclusions&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;GP ameliorates MASLD by a","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2026 1","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/3170493","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148942122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of Amylase and Cellulase Production From Cantharellus cibarius: Insights into Nutritional Composition and Biological Activities","authors":"Marwa Cheikh Rouhou,&nbsp;Nardjis Sayada,&nbsp;Manoj J. Dev,&nbsp;Mariem Itaimi Dammak,&nbsp;Hatem Majdoub,&nbsp;Abdulla Bashein,&nbsp;Nourhene Boudhrioua,&nbsp;Hamdi Bendif,&nbsp;Hassan A. Rudayni,&nbsp;Amr Elkelish,&nbsp;Stefania Garzoli,&nbsp;Salem Shamekh","doi":"10.1155/jfbc/2616806","DOIUrl":"https://doi.org/10.1155/jfbc/2616806","url":null,"abstract":"<p>This study investigated the nutritional composition, biological activities, and extracellular enzyme production potential of the edible mushroom <i>Cantharellus cibarius</i> (<i>C. cibarius</i>). Proximate analysis revealed that this mushroom was rich in carbohydrates, proteins, minerals, and dietary fibers, with cellulose representing the major fiber fraction (48.15%). Aqueous and methanolic extracts exhibited strong antioxidant activities, reaching 78.32% and 77.31% inhibition in DPPH and ABTS radical scavenging activity assays, respectively. In addition, the extracts demonstrated significant anti-inflammatory activity in vitro, as determined by the protein denaturation assay. The production of extracellular amylase and cellulase was initially screened under different culture conditions, identifying pH, incubation time, and carbon source as the main factors affecting enzyme production. Response surface methodology was subsequently applied to optimize enzyme production, resulting in maximum amylase and cellulase activities of 3.6785 U/mL and 3.3994 U/mL, respectively. Cellulase stability was then evaluated and exhibited optimal activity at pH 6.0°C and 30°C, while MnCl₂ enhanced enzyme activity and CuSO₄ and KCl showed inhibitory effects. These findings demonstrate that <i>C. cibarius</i> is a promising source of bioactive compounds and extracellular enzymes with potential applications in the food and biotechnology industries.</p>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2026 1","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/2616806","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148943602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated Multiplatform Analysis of Casein-Derived Peptides Demonstrates Epithelial Metabolic Remodeling, Immunomodulatory Activity, and Epithelial Handling","authors":"Yasuri Amarasekara,&nbsp;Mitchell Low,&nbsp;Narsimha Reddy,&nbsp;Junior Te’o,&nbsp;Anwar Sunna","doi":"10.1155/jfbc/1236139","DOIUrl":"https://doi.org/10.1155/jfbc/1236139","url":null,"abstract":"<p>The biological relevance of milk-derived bioactive peptides depends not only on their reported bioactivities but also on whether they remain biologically relevant following intestinal epithelial exposure. Here, we used an integrated multiplatform workflow to determine whether combining epithelial metabolomic profiling, immunomodulatory screening, and epithelial handling could improve the assessment of the biological relevance of casein-derived peptides. Four bovine casein–derived peptides, FVAPFPEVFG, NLLRF, EMPFPKY, and SDIPNPIGSENSEK, were evaluated using Caco-2 LC-MS/MS metabolomic profiling and RAW 264.7 immunomodulatory screening, both performed in technical duplicate (<i>n</i> = 2), together with Caco-2 epithelial handling studies. Untargeted LC-MS/MS metabolomics identified 251 differential intracellular positive-ion features, 341 extracellular positive-ion features, and 48 intracellular negative-ion features, demonstrating extensive peptide-associated metabolic remodeling. Peptide exposure was associated with remodeling of both intracellular and extracellular metabolic pathways, with the most consistent changes centered on arginine-related amino acid metabolism and linked central carbon metabolism. Although the four peptides produced distinct metabolic fingerprints, they converged on related epithelial metabolic nodes. In LPS-stimulated RAW 264.7 macrophages, the peptides showed sequence-dependent immunomodulatory activity: FVAPFPEVFG produced the strongest broad suppression of inflammatory mediators, EMPFPKY showed a selective immunomodulatory profile, SDIPNPIGSENSEK showed preliminary activity in a single-point screen, and NLLRF showed no detectable immunomodulatory activity under the tested conditions. To determine whether a multifunctional peptide remained biologically relevant following epithelial exposure, EMPFPKY was selected for Caco-2 transport studies. LC-MS/MS reproducibly detected the EMPFPK and EMPFP peptide fragments during transepithelial passage, while live-cell fluorescence imaging supported cell-associated uptake of peptide-derived material. Integrating epithelial metabolomics, immunomodulatory screening, and epithelial handling strengthens the assessment of the potential biological relevance of milk-derived bioactive peptides beyond conventional bioactivity screening. Although these findings provide mechanistic insight, they are based on complementary in vitro epithelial and macrophage models and require validation in more physiologically relevant systems.</p>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2026 1","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/1236139","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148943394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent Advances in Ant-Derived Lipids as Emerging Functional Ingredients for Food, Nutraceutical, and Sustainable Bioproduct Applications: A Comprehensive Review","authors":"Anushka Pallavi,&nbsp;Pinku Chandra Nath,&nbsp;Vinay Kumar Pandey,&nbsp;Shikha Thakur,&nbsp;Rahul Singh,&nbsp;Dipendra Kumar Mahato","doi":"10.1155/jfbc/6476186","DOIUrl":"https://doi.org/10.1155/jfbc/6476186","url":null,"abstract":"<p>Ant lipids provide a viable resource for attaining a sustainable future, due to their low pollution output and their abundance in fatty acids, phospholipids, sterols, and triglycerides. They are employed across multiple industries, including cosmetics, food, and medicines, due to their extensive health benefits, which encompass anticancer, antidiabetic, immunoprotective, cardioprotective, anti-inflammatory, and antimicrobial qualities. Research on the extraction of ant lipids has been limited. Consumers persist in demonstrating a psychological reluctance toward their usage, and there remains a lack of laws and regulations concerning their safety. This review discusses the prospective applications of lipids sourced from ants, together with the most sophisticated methods for their extraction and characterization. It also elucidates their nutritional characteristics, health advantages, and the function of these lipids in fostering a sustainable future. Ant lipids have the capacity to be included into nutraceutical compositions. Furthermore, they can be employed in the food industry as functional foods, in baked goods and confectionery, frozen products, and as substitutes for meat and dairy alternatives owing to their exceptional physical attributes, spreadability, and emulsifying capabilities. Moreover, they possess the capacity to enhance food security and attain environmental sustainability goals.</p>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2026 1","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/6476186","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148849187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"α-Phellandrene, a Celery Component, Protects Intestinal Epithelial Cells Against Oxidative and Inflammatory Stress Through Modulation of Nrf2 and NF-κB Signaling","authors":"Sovie Anggraeni,&nbsp;Laksmi Hartajanie,&nbsp;Chih-Chung Wu","doi":"10.1155/jfbc/4431967","DOIUrl":"https://doi.org/10.1155/jfbc/4431967","url":null,"abstract":"<p>α-Phellandrene (α-PA), a naturally occurring monoterpene abundant in celery and dill, has gained increasing attention as a food-derived bioactive compound with potential health benefits. However, its protective role in maintaining intestinal epithelial integrity under chemotherapy-induced stress remains insufficiently characterized. This study investigated the protective effects and underlying mechanisms of α-PA against oxidative and inflammatory stress in rat intestinal epithelial cells (IEC-6) challenged with 5-fluorouracil (5-FU). IEC-6 cells were treated with 50–250 μM α-PA and 1 mM 5-FU for 48 h to establish an experimental model for evaluating its cytoprotective effects. The study examined oxidative stress markers, inflammatory mediators, and the regulatory involvement of the nuclear factor erythroid 2–related factor 2 (Nrf2) and nuclear factor kappa B (NF-κB) signaling pathways. Results demonstrated that α-PA treatment significantly attenuated intracellular reactive oxygen species (ROS) accumulation and lipid peroxidation while raising antioxidant defenses. Specifically, α-PA upregulated intracellular glutathione (GSH) levels and the mRNA expression of heme oxygenase-1 (<i>Ho-1</i>) and glutamate-cysteine ligase subunits (<i>Gclc</i>/<i>Gclm</i>). Concurrently, α-PA suppressed inflammatory responses, as evidenced by reduced production of nitric oxide (NO) and prostaglandin E2 (PGE2), alongside decreased secretion of proinflammatory cytokines (IL-1β, IL-6, and TNF-α) while restoring anti-inflammatory IL-10 levels. These cytoprotective effects were closely associated with the coordinated activation of Nrf2 signaling and the suppression of NF-κB nuclear translocation and DNA-binding activity. From a functional food perspective, these findings suggest that α-PA may serve as a promising dietary intervention to enhance intestinal resilience against chemotherapy-induced oxidative and inflammatory damage.</p>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2026 1","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/4431967","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148849173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hesperidin, Hesperetin, and Naringenin in Retinal Pathophysiology: Molecular Crosstalk Between Metabolic Dysfunction, Neuroinflammation, and Therapeutic Signaling in Diabetic Retinopathy","authors":"Guangling Feng,&nbsp;Zhicheng Huang,&nbsp;Farzam Kiarasi","doi":"10.1155/jfbc/4871891","DOIUrl":"https://doi.org/10.1155/jfbc/4871891","url":null,"abstract":"<p>Diabetic retinopathy (DR) is a leading cause of blindness worldwide, characterized by retinal vascular dysfunction, oxidative stress, inflammation, and neuronal degeneration. Flavonoids such as hesperidin (HSD), hesperetin (HST), and naringenin, commonly found in citrus fruits, have shown promise in mitigating DR through multiple molecular mechanisms. HSD, a glycoside of HST, exhibits potent antioxidant effects by enhancing endogenous enzymes like superoxide dismutase and catalase, thereby reducing oxidative damage and reactive oxygen species accumulation. It also attenuates blood–retinal barrier breakdown, decreases advanced glycation end products, and lowers inflammatory cytokines, which may contribute to the preservation of retinal structure and function. HST, the aglycone form of HSD, demonstrates vasoprotective properties by inhibiting angiogenic pathways including the vascular endothelial growth factor and protein kinase C-beta, thereby potentially reducing retinal vascular leakage and basement membrane thickening. Naringenin exerts neuroprotective effects by modulating oxidative stress and apoptosis and may enhance neurotrophic factors such as brain-derived neurotrophic factor and tropomyosin receptor kinase B, while decreasing proapoptotic markers, thereby supporting neuronal survival. Collectively, these flavonoids represent promising candidates for targeting DR by influencing metabolic dysfunction, neuroinflammation, vascular abnormalities, and neurodegeneration.</p>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2026 1","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/4871891","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148849172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"GC–MS and MALDI-TOF-MS Integrated Techniques for a Comprehensive Evaluation of the Effect of Forsythia Fruit Essential Oil Application on Vacuum-Packaged Broccoli","authors":"Miroslava Kačániová,&nbsp;Minhang Qiao,&nbsp;Alessandro Bianchi,&nbsp;Joel Horacio Elizondo-Luevano,&nbsp;Anis Ben Hsouna,&nbsp;Rania Ben Saad,&nbsp;Zhaojun Ban,&nbsp;Li Li,&nbsp;Yizhou Gao,&nbsp;Wissem Mnif,&nbsp;Stefania Garzoli","doi":"10.1155/jfbc/4063394","DOIUrl":"https://doi.org/10.1155/jfbc/4063394","url":null,"abstract":"<p>This study provides a comprehensive evaluation of the biological activity of essential oil obtained from the fruits of <i>Forsythia suspensa</i> (Thunb.) Vahl (FFEO) of Chinese origin. The chemical composition of FFEO was determined by GC–MS analysis, revealing a profile dominated by <i>α</i>-fenchene (57.1%), which represents an unusual and previously unreported dominant constituent within the genus <i>Forsythia</i>. FFEO exhibited antimicrobial activity against all tested bacterial strains and <i>Candida</i> species, with higher susceptibility observed in Gram-positive bacteria. To the best of our knowledge, the antimicrobial activity of FFEO in the vapor phase was evaluated on fresh food matrices for the first time, demonstrating concentration-dependent inhibition on broccoli, white radish, banana, and kiwi. A storage experiment confirmed that the combination of FFEO with vacuum packaging was the most effective strategy for reducing microbial counts in broccoli during 14-day refrigerated storage. Broccoli microbiota was characterized by MALDI-TOF-MS, identifying 35 bacterial species from 13 families, with <i>Pseudomonas</i> as the dominant genus. To the best of our knowledge, insecticidal activity of FFEO was demonstrated for the first time against the storage pests <i>Callosobruchus maculatus</i> and <i>Megabruchidius dorsalis</i> in a fumigation model. Overall, FFEO shows promise as a multifunctional natural bioactive agent for food protection. To the best of our knowledge, this is the first study to evaluate the antimicrobial activity of FFEO in the vapor phase on fresh food matrices and to report its insecticidal activity against <i>C. maculatus</i> and <i>M. dorsalis</i>.</p>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2026 1","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/4063394","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148848551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}