Betulinic Acid–Mediated NR1D1 Alleviates Dextran Sulfate Sodium–Induced Colitis in Mice by Modulating VDAC1/NF-κВ

IF 3.5 2区农林科学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of Food Biochemistry Pub Date : 2025-06-13 DOI:10.1155/jfbc/3244032

Qikun Zhang, Ge Song, Rulei Zhong, Haibo Wang, Zhiqiang Liu, Haodong Cui, Jimin Yin, Yong Qi, Wenyong Wu

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引用次数: 0

Abstract

Purpose: Inflammatory bowel disease (IBD) can severely disrupt intestinal health, leading to chronic inflammation. Betulinic acid (BHA) has demonstrated anti-inflammatory and antioxidant properties. This study aims to explore the mechanism by which BHA alleviates colitis through the regulation of the key circadian gene REV-ERBα (NR1D1) in a DSS-induced colitis mouse model.

Methods: The study first utilized in vivo experiments to establish a mouse model of colitis induced by DSS, investigating the effect of BHA in alleviating colitis through the regulation of NR1D1. Subsequently, an inflammatory model was established at the cellular level using CCD841 cells treated with 100 ng/mL LPS to explore the regulatory mechanism of BHA on colitis.

Results: Our results indicate that oral administration of BHA effectively alleviates colitis symptoms, as shown by reduced disease activity index and histopathological scores. Notably, we found that BHA improves intestinal inflammation in DSS-induced mice by downregulating VDAC1/NF-κB. In addition, in vitro experiments demonstrate that the anti-inflammatory effects of BHA are closely related to the NR1D1 gene.

Conclusion: Our findings demonstrate the potential of BHA as a preventive and therapeutic agent for IBD.

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白桦酸介导的NR1D1通过调节VDAC1/NF减轻硫酸葡聚糖钠诱导的小鼠结肠炎-κВ

目的：炎症性肠病（IBD）可严重破坏肠道健康，导致慢性炎症。白桦酸（BHA）具有抗炎和抗氧化的特性。本研究旨在探讨BHA在dss诱导的结肠炎小鼠模型中通过调节关键昼夜节律基因rev - erba （NR1D1）减轻结肠炎的机制。方法：本研究首先采用体内实验建立DSS诱导结肠炎小鼠模型，研究BHA通过调控NR1D1减轻结肠炎的作用。随后，采用100 ng/mL LPS处理CCD841细胞，建立细胞水平炎症模型，探讨BHA对结肠炎的调节机制。结果：我们的研究结果表明，口服BHA可有效缓解结肠炎症状，降低疾病活动指数和组织病理学评分。值得注意的是，我们发现BHA通过下调VDAC1/NF-κB来改善dss诱导小鼠的肠道炎症。此外，体外实验表明，BHA的抗炎作用与NR1D1基因密切相关。结论：我们的研究结果证明了BHA作为IBD的预防和治疗药物的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Food Biochemistry 生物-生化与分子生物学

CiteScore

7.80

自引率

5.00%

发文量

488

审稿时长

3.6 months

期刊介绍： The Journal of Food Biochemistry publishes fully peer-reviewed original research and review papers on the effects of handling, storage, and processing on the biochemical aspects of food tissues, systems, and bioactive compounds in the diet. Researchers in food science, food technology, biochemistry, and nutrition, particularly based in academia and industry, will find much of great use and interest in the journal. Coverage includes: -Biochemistry of postharvest/postmortem and processing problems -Enzyme chemistry and technology -Membrane biology and chemistry -Cell biology -Biophysics -Genetic expression -Pharmacological properties of food ingredients with an emphasis on the content of bioactive ingredients in foods Examples of topics covered in recently-published papers on two topics of current wide interest, nutraceuticals/functional foods and postharvest/postmortem, include the following: -Bioactive compounds found in foods, such as chocolate and herbs, as they affect serum cholesterol, diabetes, hypertension, and heart disease -The mechanism of the ripening process in fruit -The biogenesis of flavor precursors in meat -How biochemical changes in farm-raised fish are affecting processing and edible quality