Journal of Food Biochemistry最新文献

{"title":"Preparation, Identification, and Digestive Stability of Antioxidant Peptides From Chlorella vulgaris","authors":"Yan Wang,&nbsp;Xiao Kong,&nbsp;Mengdi Du,&nbsp;Yang Qiao,&nbsp;Minjie Ye,&nbsp;Bing Han,&nbsp;Jing Gan","doi":"10.1155/2024/3828251","DOIUrl":"https://doi.org/10.1155/2024/3828251","url":null,"abstract":"<div>\u0000 <p><i>Chlorella</i>, a single-celled green algae rich in proteins, holds promise as a source of bioactive peptides. This research aimed to isolate an antioxidant peptide from <i>Chlorella</i>, examining its stability through digestion. Using Response Surface Methodology (RSM), we identified optimal hydrolysis conditions for <i>Chlorella</i> Protein Hydrolysate (CPH): 55°C, pH 8.75, and an enzyme-to-substrate ratio of 2.15%. These conditions led to CPH with notable radical scavenging activity against 1,1-diphenyl-2-picrylhydrazyl (DPPH) (53.19 ± 0.99%), 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) (76.86 ± 0.28%), hydroxyl radical (53.39 ± 1.74%), and Fe²⁺ chelating rates of 22.56 ± 2.97%. Subsequently, peptides under 3 kDa isolated by ultrafication proved particularly stable and antioxidative after <i>in vitro</i> digestion. In addition, further purify to obtain component C1 with DPPH radical scavenging ability of 59.62 ± 1.29% and an Fe^<i>2+</i> chelating ability of 49.78 ± 1.55%. Subsequent LC-MS/MS identification and virtual screening led to the discovery of a stable and antioxidative peptide, Ser-Gly-His-His-Lys-Pro-Leu (SGHHKPL). The study provides the theoretical basis for the development and utilization of <i>chlorella</i>.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2024 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2024/3828251","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142524997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of Polysulfides in Aged Sparkling Wines From Different Vintages","authors":"Susanne Dekker,&nbsp;Tiziana Nardin,&nbsp;Tomas Roman,&nbsp;Roberto Larcher","doi":"10.1155/2024/3795283","DOIUrl":"https://doi.org/10.1155/2024/3795283","url":null,"abstract":"<div>\u0000 <p>Traditional sparkling wines are obtained from a vinification process with an additional ‘second’ fermentation in the bottle, which has an essential impact on the sensorial characteristics. During this maturation phase, several compounds are released from the yeast in autolysis, including the tripeptide glutathione. This compound has recently gained more interest due to its role in the formation of polysulfides and the subsequent possibility of H₂S release post-bottling. Hence, the release of sulphur-containing compounds like glutathione during an ageing ‘sur lies’ ought to be considered regarding the accumulation and persistence of polysulfides. In this study, the presence of polysulfides in traditional sparkling wines was validated to gain a preliminary understanding of the role of ageing time and glutathione. UHPLC-HRMS allowed the detection of two glutathionyl trisulfides in a selection of different naturally sparkling wines. Besides, it was found that the wines from vintage 2020 had a relatively high formation of GS₃G and GS₃C compared to vintages 2018 and 2019. Moreover, a trend between the trisulfides and GSSC was observed, possibly related to glutathione condensation and cysteine uptake by the yeast. This study showed the presence of polysulfides in various sparkling wines for the first time, increasing the availability of the current knowledge on this topic.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2024 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2024/3795283","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142524996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Botanical Sources, Pharmacokinetics, and Therapeutic Efficacy of Palmatine and Its Derivatives in the Management of Cancer: A Comprehensive Mechanistic Analysis","authors":"Most. Israt Jahan Oni,&nbsp;Md. Shimul Bhuia,&nbsp;Raihan Chowdhury,&nbsp;Salehin Sheikh,&nbsp;Md. Hanif Munshi,&nbsp;Md. Sakib Al Hasan,&nbsp;Muhammad Torequl Islam","doi":"10.1155/2024/8843855","DOIUrl":"https://doi.org/10.1155/2024/8843855","url":null,"abstract":"<div>\u0000 <p>Natural compounds and their derivatives have been identified as valuable sources of therapeutic ingredients for cancer treatment. The naturally occurring phytochemical palmatine (isoquinoline alkaloid) is extracted from plant parts (rhizomes, roots, stems, stem barks, and others) and has protective effects including antioxidant, anti-inflammatory, and anticancer. This study aims to summarize the anticancer potential of palmatine and its derivatives in the treatment of numerous types of cancer with molecular mechanisms. We also include the pharmacokinetic features, botanical origin, and toxicological characteristics of palmatine and its derivatives. For this, data have been collected from plausible different electronic databases, including PubMed, Google Scholar, PubChem, Science Direct, Web of Science, Scopus, Springer Link, and Wiley Online. The findings demonstrate that palmatine and its derivatives have a protective anticancer effect against a variety of cancers, including breast, colorectal, gastric, ovarian, prostate, pancreatic, skin, hepatocellular cancer, and mammary gland tumors. They provoke their anticancer properties against various cancer cell lines via modifying molecular mechanisms like induction of oxidative stress, cytotoxicity, apoptosis, inhibition of cell invasion and migration, arresting the cell cycle at the S phase, antiproliferative, and antiangiogenic effects. It is suggested that palmatine and its derivatives may be a good option in the development of novel drugs for cancer therapy in the future.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2024 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2024/8843855","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142524995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protection of Stored Common Bean and Maize Grains Using Optimally Synthesized Biosilica from Rice Husk Ash","authors":"Bertrand Zing Zing,&nbsp;Lin Marcellin Messi Ambassa,&nbsp;Eugene Ehabe Ejolle,&nbsp;Desire Placide Belibi Belibi,&nbsp;Charles Melea Kede","doi":"10.1155/2024/3741615","DOIUrl":"https://doi.org/10.1155/2024/3741615","url":null,"abstract":"<div>\u0000 <p>Losses provoked by insect pests on stored cereals and legumes contribute immensely to reduce food security, especially in rural communities in Sub-Saharan Africa. The application of integrated control strategies, involving the use of synthetic pesticides, is often expensive and hazardous to humans and the environment. This study was conducted to optimize the production and use of biosilica from rice husk ashes, for the control of <i>Sitophilus zeamais</i> and <i>Acanthoscelides obtectus</i>, major insect pests of stored maize and common bean grains. The amorphous nature of the derived silica molecules coupled with the silanol and siloxane groups on their surfaces seemed to account for silica’s insecticidal effects on the pests, which were more on <i>A. obtectus</i> on common beans than <i>S. zeamais</i> on maize grains, with full mortalities obtained at doses of about 1.25 g per 50 g bean seeds and about 2.25 g per 50 g maize seeds, within 3 and 12 days, respectively. Similarly, the daily lethal dose (LD₅₀) of silica powder required to obtain the mortality of half of the insects was lower on common beans than on maize seeds. The reduced phytotoxic activity of the biosilica on the stored common bean and maize grains indicates therefore that it could make an ecofriendly alternative to conventional protectants for small quantities of stored grains. However, large-scale studies are needed in order to evaluate its potential use in grain storage systems.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2024 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2024/3741615","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142451758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating Nudaurelia dione (Saturniidae), an Edible Insect, for Sustainable Nutrition: Composition, Benefits, and Antinutritional Insights","authors":"Adjoua Christiane Eunice Boko,&nbsp;Sika Hortense Blei,&nbsp;Anauma Casimir Koko,&nbsp;Djédoux Maxime Angaman","doi":"10.1155/2024/5559567","DOIUrl":"https://doi.org/10.1155/2024/5559567","url":null,"abstract":"<div>\u0000 <p>Increasingly, insects are heralded as a sustainable and efficacious solution to global challenges of malnutrition and protein scarcity. The Food and Agriculture Organization (FAO) advocates for the use of insects as a sustainable substitute for conventional animal-based proteins, in response to the escalating pressures on natural resources and the environment. This study focuses on <i>Nudaurelia dione</i>, exploring its nutritional and antinutritional profiles through rigorous laboratory analyses employing standardized methodologies. Our findings establish that <i>N. dione</i> is a potent source of macronutrients, providing significant quantities of proteins, lipids, and carbohydrates, with a calculated energy content of 413.64 ± 13.32 kcal per 100 g of dry matter (DM). The lipid fraction is particularly rich in essential fatty acids, including <i>α</i>-linolenic and linoleic acids. Additionally, the insect species contains high levels of essential minerals such as calcium, magnesium, sodium, and potassium. Despite these nutritional benefits, the presence of considerable amounts of phytates (2059.96 ± 5.12 mg per 100 g of DM) poses potential health risks. Therefore, it is imperative to develop and apply appropriate processing methods to reduce these antinutritional factors effectively.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2024 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2024/5559567","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142447784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolomic Profiling Using UPLC‒MS/MS Revealed Differential Changes in Metabolites of Three Tea Plant Varieties and Their Potential Antioxidant Activities","authors":"Lunxian Liu,&nbsp;Xinglin Wang,&nbsp;Qiuying Li,&nbsp;Han Dai,&nbsp;Qihang Zhou,&nbsp;Xingpan Meng,&nbsp;Zhongting Chen,&nbsp;Ximin Zhang,&nbsp;Zhengdong Zhang,&nbsp;Yingliang Liu,&nbsp;Tie Shen,&nbsp;Yin Yi","doi":"10.1155/2024/5566593","DOIUrl":"https://doi.org/10.1155/2024/5566593","url":null,"abstract":"<div>\u0000 <p>Tea plants, as essential health beverages, are widely recognized for their rich content of antioxidant metabolites. This study utilized metabolomics analysis techniques to compare the metabolic differences between <i>Camellia tachangensis</i> F. C. Zhang, <i>Camellia gymnogyna</i> Hung T. Chang, and <i>Camellia sinensis</i> (L.) O. Kunzte, aiming to reveal their potential antioxidant activities. By applying UPLC–MS/MS technology, we analyzed metabolites in the samples and identified 1056 metabolites. We utilized statistical methods such as orthogonal partial least squares discriminant analysis (OPLS-DA) and principal component analysis to evaluate and compare the variations in the samples. An intersection analysis of metabolites from the three types of tea plants identified 29 major differential metabolites. Functional annotation and pathway analysis of these differential metabolites were conducted, ultimately discussing 24 antioxidant-related metabolites, primarily belonging to flavonoids, phenolic acids, and other categories. Among the three types of tea, <i>C. tachangensis</i> has the highest content of procyanidin B4, which is the most abundant substance in this tea. In terms of the impact of this substance on tea, it is known to have antioxidant properties and contributes to the overall antioxidant capacity of tea. In both <i>C. gymnogyna</i> and <i>C. sinensis</i>, the top 5 antioxidant metabolites include substances such as apigenin-6,8-di-C-glucoside (vicenin-2), epigallocatechin, and apigenin-5-O-glucoside. These substances belong to the flavonoid class and contribute to the antioxidant properties of these teas. Beyond the flavonoids and phenolic acids, other classes of metabolites in tea plants also exhibited significant antioxidant properties. These different metabolites play a key role in the antioxidant function of tea, contributing to their potential health benefits. The metabolites provide crucial insights for exploring and developing high-antioxidant products that occur naturally.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2024 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2024/5566593","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142439071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association Between Pleurotus ostreatus Consumption and More Optimal Sports Performance: A Narrative Review","authors":"Nazila Parnian-Khajehdizaj,&nbsp;Elena Mainer Pardos,&nbsp;Steven B. Machek,&nbsp;Hadi Nobari","doi":"10.1155/2024/2610415","DOIUrl":"https://doi.org/10.1155/2024/2610415","url":null,"abstract":"<div>\u0000 <p>In the past few years, much research has been conducted on edible mushrooms, among which <i>Pleurotus ostreatus</i> (PO)—also known as oyster mushrooms—has received attention due to its many health and medicinal benefits. Some research has shown that PO and its active compounds, such as lectins, polysaccharides, primarily <i>β</i>-glucans, phenols, polyphenols, terpenoids, ergosterols, and glycoproteins, are strong immune system modulators, have anti-inflammatory properties, and have potentially positive effects on the cardiovascular system and athletic performance. In this narrative review, an extensive search (academic databases from inception to April 25, 2024) was conducted in the scientific literature, and various aspects of PO and its effects on health and athletic performance were investigated. This narrative review article thereby demonstrates the potential benefits of PO for improving the overall baseline health and concomitant athletic sports performance, recovery, and cardiovascular system function. In addition to potential enhancements in antioxidant defense, substrate metabolism, and being a rich source of many essential nutrients, we describe a possible positive relationship between PO intake and improved athletic performance and recovery in athletes after intense exercise by reducing inflammation, modulating the microbiome, neutralizing free radicals, and strengthening the cardiovascular system. However, more research in basic studies and clinical trials is needed to investigate PO’s clinical applications, mechanisms, and effects on athletes.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2024 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2024/2610415","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142429899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chlorogenic Acid Mitigates Ferroptosis by Activating the Nrf2/GPX4 Pathway through Keap1 Blockade in Vascular Dementia Rats","authors":"Wen Shan,&nbsp;Guodong Liu,&nbsp;Chulei Deng,&nbsp;Yu Wei,&nbsp;Ke Ding","doi":"10.1155/2024/7848982","DOIUrl":"https://doi.org/10.1155/2024/7848982","url":null,"abstract":"<div>\u0000 <p>Chlorogenic acid (CGA) is a dietary phenolic acid widely distributed in daily food and plants, but its role in vascular dementia (VaD) is still unclear. Hence, this study aimed to investigate whether CGA could rescue cognitive impairment in VaD, providing a new option for drug discovery. Novel object recognition and Morris water maze experiments revealed that CGA enhanced the learning and memory abilities in VaD rats. Nissl staining, Western blot, and transmission electron microscopy results demonstrated that CGA inhibited neuronal loss in the hippocampal CA1 region of VaD rats, increased the expression of synaptic-related markers SYP and PSD95, thickened the postsynaptic density, and suppressed mitochondrial ridge rupture in neurons. GSH detection, MDA detection, and Western blot experiments indicated that CGA alleviated hippocampal GSH reduction and MDA elevation and increased the protein levels of GPX4 and SLC7A11. Cell activity, ROS detection, lipid peroxidation detection, intracellular Fe²⁺ level detection, GSH detection, and Western blot revealed that CGA inhibited the increase of intracellular Fe²⁺, ROS, and lipid peroxidation induced by OGD in PC12 cells, mitigated GSH reduction, and increased the protein levels of GPX4, SLC7A11, SYP, and PSD95. Western blot and immunofluorescence staining showed that CGA increased the expression of pSer40-Nrf2 in the hippocampal tissue of VaD rats and PC12 cells, promoting Nrf2 nuclear translocation. Knockdown of Nrf2 prevented CGA from rescuing ferroptosis and synaptic damage induced by OGD in PC12 cells, as well as Nrf2 pathway activation. Molecular docking analysis suggested that CGA competitively bound to the Kelch domain of Keap1 with Nrf2 and then promoted Nrf2 release and activated downstream signaling pathways. In conclusion, our study suggests that CGA may activate the Nrf2 signaling pathway by disrupting the interaction between Nrf2 and Keap1 proteins, thereby inhibiting hippocampal neuronal ferroptosis and synaptic damage and ameliorating cognitive impairment in VaD. <i>Practical Applications</i>. Vascular dementia (VaD) stands as the second most prevalent type of dementia following Alzheimer’s disease. However, there is no effective clinical treatment drug available, and its pathogenic mechanisms remain elusive. This study proposed that CGA might activate the Nrf2-GPX4 signaling pathway by competitively binding to Keap1, thereby attenuating hippocampal neuronal ferroptosis and synaptic damage and consequently ameliorating cognitive impairment in VaD. Our study may provide a novel option for drug research and development for VaD.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2024 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2024/7848982","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142429621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Negative Effects of Cx43 during Denervated Skeletal Muscle Atrophy Can Be Attenuated by Blueberry Extracts via Regulating Akt Pathway","authors":"Xinying Huang,&nbsp;Jibo Liu,&nbsp;Junpeng Zhang,&nbsp;Zongqi You,&nbsp;Yaoxian Xiang,&nbsp;Junxi Dai,&nbsp;Lei Xu,&nbsp;Junjian Jiang,&nbsp;Jianguang Xu","doi":"10.1155/2024/8830689","DOIUrl":"https://doi.org/10.1155/2024/8830689","url":null,"abstract":"<div>\u0000 <p>The lack of effective treatments for alleviating irreversible skeletal muscle atrophy caused by peripheral nerve dysfunction is a clinical problem. To identify therapeutic targets, signaling pathways in muscle cells activated by denervation have been explored, including the important roles of Cx43, a kind of protein that forms gap junctions, in other types of pathological process. The present study investigated the negative role of Cx43 in aggravated muscle cell degeneration following denervation. The blueberry extract was applied orally on mice models, and its protective effect against denervated skeletal muscle atrophy was found by wet weight measurement and tissue staining observation. Cx43/Akt pathway in denervated muscle cells was modulated by blueberry extract. In conclusion, the negative effects of Cx43 on denervated skeletal muscle atrophy were attenuated by blueberry extract by regulating the Akt pathway.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2024 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2024/8830689","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142429015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structural Characterization and Anti-Inflammatory Activity of Novel Acidic Polysaccharides from Laoshan Black Tea","authors":"Hong Gao,&nbsp;Rongshen Wang,&nbsp;Yuxin Wang,&nbsp;Huiting Li,&nbsp;Shuying Lan,&nbsp;Mengyao Zhao,&nbsp;Xiaoxiao Liu,&nbsp;Wanzhong Li","doi":"10.1155/2024/4709041","DOIUrl":"https://doi.org/10.1155/2024/4709041","url":null,"abstract":"<div>\u0000 <p>In LPS-stimulated mouse inflammation model, Laoshan black tea polysaccharides (BTPSs) showed significant anti-inflammatory activity. Three water-soluble acidic polysaccharides (BTPS 3-1, BTPS 3-2, and BTPS 3-3) were purified. They contained glucose (Glc), galactose (Gal), fucose (Fuc), rhamnose (Rha), glucuronic acid (GlcA), and mannose (Man) at molar ratios of 9.0 : 4.9 : 4.9 : 1.7 : 1.5 : 1.0, 9.5 : 5.3 : 4.9 : 1.7 : 1.6 : 1.0, and 5.3 : 3.5 : 2.0 : 2.1 : 0.9 : 1.0 with average molecular weights of 28.38, 14.39, and 11.00 kDa, respectively. Three polysaccharides reduced the levels of NO, TNF-<i>α</i>, and IL-6 in LPS-induced RAW 264.7 cells, and BTPS 3-2 had the strongest anti-inflammatory effects. Based on analyses of the monosaccharide composition, methylation, and NMR, the main chain of BTPS 3-2 was composed of ⟶4) -<i>β</i>-D-Glc<i>p</i>- (1⟶, ⟶2) -<i>α</i>-L-Fuc<i>p</i>- (1⟶, and ⟶6) -<i>β</i>-D-Gal<i>p</i>- (1⟶, and Rha<i>p</i>, Glc<i>p</i>A, and Man<i>p</i> units were attached as side chains to the backbone. BTPS 3-2 also downregulated the expression levels of p-NF-<i>κ</i>B p65, p-I<i>κ</i>B<i>α</i>, TLR4, MyD88, COX-2, TNF-<i>α</i>, and IL-6. BTPS 3-2 ameliorated LPS-induced inflammation by regulating the TLR4/MyD88/NF-<i>κ</i>B signaling pathway.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2024 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2024/4709041","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142429188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}