Journal of Food Biochemistry最新文献

{"title":"Inhibitory Effects and Mechanisms of Water-Soluble Chitosan/Curdlan Edible Composite Coating on Polygalacturonase and β-Glucosidase","authors":"Kunyu Liu,&nbsp;Ziyu Jing,&nbsp;Jiasi Li,&nbsp;Jiaqi Chang,&nbsp;Hesheng Wang,&nbsp;Silong Jia,&nbsp;Youwei Yu,&nbsp;Shaoying Zhang","doi":"10.1155/jfbc/8884163","DOIUrl":"https://doi.org/10.1155/jfbc/8884163","url":null,"abstract":"<div>\u0000 <p>Postharvest fruits such as cherry tomato (<i>Lycopersicon esculentum</i> var. cerasiforme A. Gray) are prone to rot owing to the infection of pathogens, leading to great economic losses to farmers. To investigate the inhibitory effect and mechanism of polygalacturonase and β-glucosidase activity in cherry tomatoes and pathogenic fungi such as <i>Alternaria</i> (Nees: Fr) and <i>Botrytis cinerea</i>, various analyses including molecular simulation, ultraviolet spectrum, fluorescence spectrum, circular binary chromatography, inhibition rate, and enzymatic activity test were studied. The results showed that composite coating of water-soluble chitosan (CTS)/curdlan (CUR) significantly inhibited the activities of polygalacturonase and β-glucosidase in cherry tomatoes, <i>Alternaria (Nees: Fr)</i>, and <i>Botrytis cinerea</i>. In addition, composite coating induced changes in enzyme conformation leading to a decrease in the rate of cell wall degradation in cherry tomatoes. The composite membrane inhibited 85% of polygalacturonase and 88% of the polygalacturonase and the ASP ASN ARG PRO GLU GLN and TRP residue of the β-glucosidase, thereby significantly inhibiting the decay of cherry tomatoes. In conclusion, the composite coating might inhibit the enzyme activity altering the enzyme structure, which showed promise as an effective method for fruit preservation.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2025 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/8884163","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143380108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"UVB-Protective Effects and Its Mechanisms of Vitis amurensis Rupr. Methanol Extract in Human Primary Dermal Fibroblasts","authors":"Nam Hyung Lim,&nbsp;Sae Woong Oh,&nbsp;Kitae Kwon,&nbsp;Eunbi Yu,&nbsp;See-Hyoung Park,&nbsp;Jae Youl Cho,&nbsp;Jongsung Lee","doi":"10.1155/jfbc/1507603","DOIUrl":"https://doi.org/10.1155/jfbc/1507603","url":null,"abstract":"<div>\u0000 <p>Cumulative exposure of the skin to ultraviolet light causes skin photoaging by breaking down the extracellular matrix (ECM), degrading Type I procollagen (COL1A1), and enhancing the proinflammatory cytokine levels in the skin. The extract of the herb <i>Vitis amurensis Rupr.</i> (VA) was traditionally used therapeutically owing to its antitumor and antimicrobial activities; however, its UV-protective effect on skin remains unclear. This study was aimed to explore the photoprotective effect of VA in human dermal fibroblast (HDF) and elucidated the underlying molecular pathway. We quantified the total phenolic/flavonoid content and evaluated the free radical scavenging, antiwrinkling, and anti-inflammatory effects of the VA extract at nontoxic concentrations in UVB-irradiated HDFs. We found that the VA extract scavenged free radicals and cellular reactive oxygen species (ROS), downregulated matrix metalloproteinases-1 (MMP-1) expression, restored Type I procollagen expression at the gene and protein levels, and reduced the proinflammatory cytokine IL-6 expression. Mechanistically, VA primarily downregulated the ERK and JNK pathways to regulate MMP-1 expression and activated the Smad pathway by suppressing the ERK pathway for COL1A1 synthesis. Moreover, we identified ε-viniferin as the major ingredient in the VA extract and that it exerted Type I procollagen expression-promoting activity, indicating its role as the effective compound in the VA extract. These findings suggest that VA is a photoprotective biomaterial that can be used in the cosmetics industry.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2025 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/1507603","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143380200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Analysis of Total Antioxidant Status (TAS) of Ready-to-Consume Turkish Coffees With Different Flavoring Agents","authors":"Duygu Ağagündüz,&nbsp;Zilal Ağbal,&nbsp;Dilara Sarikaya,&nbsp;Belgin Sağit,&nbsp;Teslime Özge Şahin","doi":"10.1155/jfbc/2724033","DOIUrl":"https://doi.org/10.1155/jfbc/2724033","url":null,"abstract":"<div>\u0000 <p>The production and consumption of both traditional and flavored Turkish coffee varieties are widespread in several countries, including Turkey. However, the health effects of flavorings used to enhance the flavor of commercially flavored coffees remain unclear. The aim of this study was to evaluate the total antioxidant status (TAS), a biomarker of health, in plain and commercially flavored Turkish coffees (blackberry, wild strawberry, hazelnut, vanilla, rose Turkish delight, and chocolate) commonly consumed by the Turkish population. In this study, a total of 24 different coffee samples of 6 different brands with plain, blackberry, wild strawberry, hazelnut, vanilla, rose delight, and chocolate flavors were examined. TAS analysis of the samples was performed with a commercial test kit based on (2,2′-azino-di-(3-ethylbenzthiazoline sulphonate) (ABTS) radical scavenging activity, and the results were expressed in mmol Trolox equivalent (TE)/L. The results showed that blackberry-flavored(3.14 ± 0.07 mmol TE/L) and vanilla-flavored (3.13 ± 0.07 mmol TE/L) Turkish coffees exhibited lower TAS levels compared to plain Turkish coffee (3.23 ± 0.01 mmol TE/L) and coffees flavored with wild strawberry (3.24 ± 0.04 mmol TE/L), chocolate (3.22 ± 0.01 mmol TE/L), hazelnut (3.22 ± 0.01 mmol TE/L), and rose delight (3.23 ± 0.01 mmol TE/L) (<i>p</i> &lt; 0.05). In conclusion, individuals who want to diversify their Turkish coffee consumption while maintaining or improving their health may prefer wild strawberry-, chocolate-, hazelnut-, and rose delight–flavored coffees over blackberry- and vanilla-flavored coffees. This study highlights the potential health benefits of certain flavored coffees as an alternative to traditional plain Turkish coffee.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2025 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/2724033","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143362681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Valorization of Bael Fruit Shell to Natural Emulsifiers: Formulation of Emulsifier Stabilized Chia Seed Oil-Loaded Antimicrobial Nanoemulsion Against Food Pathogenic Microorganisms","authors":"Madhu Sharma,&nbsp;Rushikesh Subhash Pharate,&nbsp;Aarti Bains,&nbsp;Kandi Sridhar,&nbsp;Vijai Kumar Gupta,&nbsp;Sanju Bala Dhull,&nbsp;Nemat Ali,&nbsp;Mohammad Khalid Parvez,&nbsp;Prince Chawla,&nbsp;Minaxi Sharma","doi":"10.1155/jfbc/8842775","DOIUrl":"https://doi.org/10.1155/jfbc/8842775","url":null,"abstract":"<div>\u0000 <p>Natural emulsifiers originating from unconventional sources have attracted attention due to their exceptional functional properties. Bael fruit shell is typically considered an agricultural byproduct and is often discarded as waste. This research, therefore, focuses on valorizing it by developing bael fruit shell extract (BFSE). The utilization of bael fruit shells makes this approach both a sustainable and functional innovation. Proximate analysis showed that bael fruit shell powder (BFSP) is rich in carbohydrates (57.89 ± 0.32%) and proteins (7.53 ± 0.90%). Compounds, including polysaccharides, proteins, and other bioactive components, were extracted from BFSP at different pH levels (4, 5, 6, and 7) with microwave treatment (950 W for 3 min). The optimal pH was identified as 5, yielding the highest soluble fractions (80.08 ± 0.46%), polysaccharide, and protein content (180.98 ± 0.34 and 56.98 ± 0.37 mg/g). The optimized BFSE powder (pH 5) showed a porous structure, excellent thermal stability, high water and oil absorption capacities, and excellent emulsifying activity and stability. The BFSE nanoemulsion showed excellent stability compared to the conventional lecithin-based nanoemulsion. The BFSE-based nanoemulsion effectively reduced the viability of <i>S. aureus</i>, <i>E. coli</i>, and <i>Candida albicans</i>, suggesting its potential as an antimicrobial agent that could be utilized for enhancing food safety and extending shelf life in food preservation.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2025 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/8842775","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143362682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Flavor Quality Characterization of Rapeseed Oil During Storage by Physicochemical Analysis, Sensory Evaluation, Electronic Nose, and GC–O","authors":"Xiumin Zhang,&nbsp;Wenfeng Du,&nbsp;Danni Zhang,&nbsp;Na Zhang,&nbsp;Yuan Liu,&nbsp;Shui Jiang","doi":"10.1155/jfbc/7434957","DOIUrl":"https://doi.org/10.1155/jfbc/7434957","url":null,"abstract":"<div>\u0000 <p><b>Background:</b> Rapeseed oil is the most widely consumed vegetable oil globally. However, the key aroma-active compounds of rapeseed oil and their changes during storage are unclear. In this study, the flavor of rapeseed oil during storage was characterized by physicochemical analysis, sensory evaluation, electronic nose (E-nose), and gas chromatography–olfactometry (GC–O).</p>\u0000 <p><b>Results:</b> Peroxide value, acid value, and anisidine value gradually increased, while polyphenol content, tocopherol content, and sterol content showed a downward trend during storage. The E-nose combined with the linear discriminate analysis (LDA) method could discriminate rapeseed oil with different storage times. The sensory attributes changed significantly from distinctive pickled aroma to rancid, green, and fried aromas during storage. This work provides the aroma-active markers based on GC–O–MS for the quality evaluation of rapeseed oil during storage. A total of 136 volatile compounds were detected by GC–MS, and 16 odorants were identified by GC–O combined with aroma extract dilution analysis (AEDA). Finally, seven aroma-active volatile compounds (3-butenyl isothiocyanate, 2(5H)-furanone, 2-methoxy-4-vinylphenol, (<i>E</i>)-2-octenal, (<i>E,E</i>)-2,4-heptadienal, (<i>E,E</i>)-2,4-decadienal, and 3-methyl-pentanoic acid) with the odor activity values (OAVs) greater than 1 were identified as potential key aroma volatiles that contributed significantly to the overall aroma of rapeseed oil.</p>\u0000 <p><b>Conclusion:</b> This study provided a comprehensive method to monitor the flavor quality change of rapeseed oil during storage. The identified volatile compounds could be the markers to characterize the quality changes of rapeseed oil during storage.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2025 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/7434957","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143248887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Characterization of Nanoliposomes of Oleaster (Elaeagnus angustifolia) Extract and Their Effect on the Oxidative Stability of Low-Fat Mayonnaise","authors":"Sara Sanei,&nbsp;Alireza Shahab Lavasani,&nbsp;Nasim Khorshidian,&nbsp;Orang Eyvazzadeh","doi":"10.1155/jfbc/4091024","DOIUrl":"https://doi.org/10.1155/jfbc/4091024","url":null,"abstract":"<div>\u0000 <p>Due to the high fat content and the use of synthetic antioxidants in the mayonnaise formulation, in this study, nanoliposomes (NLPs) of oleaster (<i>Elaeagnus angustifolia</i> L.) extract were used, and the physical and structural properties of the mayonnaise were investigated. In the research’s second phase, the quince seed mucilage (QSM) and soybean protein isolate (SPI) at concentrations of 1% and 2% were used in mayonnaise as a fat substitute. The NLPs were added to the low-fat mayonnaise formulation at concentrations of 0.5%, 0.75%, 1%, 1.5%, and 2%. Two samples without additives and TBHQ (200 ppm) were produced as a control. The samples were kept at 4°C for 6 months. The highest encapsulation efficiency, zeta potential, polydispersity index (PDI), antioxidant activity, and the smallest particle size were observed in the NLP-500 NLP (<i>p</i> &lt; 0.05). In differential scanning calorimetry and thermogravimetry thermograms, NLP-1000 showed the lowest thermal stability and weight loss. Scanning electron microscope (SEM) and transmission electron microscope (TEM) images showed that NLPs are spherical and homogeneous. Fourier transform infrared spectroscopy (FTIR) analysis confirmed the entrapment of extract in phospholipid layers and a slight change in the peaks. All control treatments showed the highest fat content and pH (<i>p</i> &lt; 0.05). TBHQ and treatments containing 2% QSM + SPI and 2% NLP (Sample 10) and 1% QSM + SPI and 2% NLP (Sample 5) showed the lowest acid, peroxide, thiobarbituric acid, and totox values during storage (<i>p</i> &lt; 0.05). Therefore, NLPs containing oleaster extract can prevent the oxidation in mayonnaise.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2025 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/4091024","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143111709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Metabolites and Biological Activities of Areca Nut (Areca catechu L.) Under Different pH: Untargeted Mass Spectrometry–Based Metabolomics Approach","authors":"Jing Zhang,&nbsp;Jiahui Dai,&nbsp;Shiping Wang,&nbsp;Kai Rui,&nbsp;Wenting Dai,&nbsp;Xiaoning Kang,&nbsp;Jianbang Ji,&nbsp;Weizhong Wang","doi":"10.1155/jfbc/9920574","DOIUrl":"https://doi.org/10.1155/jfbc/9920574","url":null,"abstract":"<div>\u0000 <p>Chewing areca nuts (ANs) produces a mild sense of excitement and happiness, particularly under alkaline conditions. To identify the substances responsible for these effects, we conducted a nontargeted metabolomics analysis comparing AN metabolites at different pH levels. Subsequently, AN’s active components were determined, and the in <i>vitro</i> antioxidant activities were evaluated. The results showed that pH affected the expression of flavonoid and isoflavonoid biosynthesis pathways, leading to changes in flavonoid, alkaloid, and organic acid levels. Alkaline conditions facilitated the hydrolysis of arecoline and guvacoline to arecaidine and guvacine, respectively. Twenty-eight alkaloids were identified as AN metabolites, of which 22 are reported for the first time. Among these, arecoline, arecaidine, scopolamine, and theobromine may be responsible for the excitatory effects of AN. The active component content and antioxidant activity of AN were significantly influenced by pH. The antioxidant activity of AN was highest at pH 12, which might be another reason AN consumer products require alkaline conditions. The excitatory effects of AN under alkaline conditions are likely owing to the combined effects of various active ingredients. In conclusion, we clarified the effect of pH on the composition and activity of AN and determined the biochemical basis of the excitatory effect of AN. These findings provide theoretical guidance for further development and utilization of active AN functions.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2025 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/9920574","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143111708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Green Production Pathways, Instability, and Stability of Resveratrol: A Systematic Review","authors":"Xingyue Jiang,&nbsp;Leilei Zuo,&nbsp;Song Gao,&nbsp;Qiong Yang,&nbsp;Ying Li,&nbsp;Yan Chen,&nbsp;Xiaofang Xie,&nbsp;Cheng Peng","doi":"10.1155/jfbc/8210896","DOIUrl":"https://doi.org/10.1155/jfbc/8210896","url":null,"abstract":"<div>\u0000 <p>Resveratrol (RES) is a nonflavonoid polyphenolic compound present in various plant species. Extensive research has confirmed the diverse biological activities of RES, rendering it highly promising for multiple industries. However, its stability is influenced by intrinsic factors, such as structure, and extrinsic factors, including light exposure, oxygen exposure, elevated temperature, and pH variations. RES also faces challenges related to low solubility, rapid metabolism, and limited bioavailability, which impede its application in the food and medical sectors. The objective of this study was to provide a comprehensive overview of the most up-to-date advancements in green and efficient RES production pathways and to evaluate RES instability and strategies for stabilization. The green and efficient production of RES is mainly achieved through plant extraction and biosynthesis. The RES plant extraction methods include deep eutectic solvent (DES) methods, ultrasound-assisted, supercritical fluid extraction (SFE), and enzyme-assisted extraction technology. The advantages and disadvantages of each method are compared in this paper. The biosynthesis of RES can produce a high yield in a short period, and the use of genetic engineering and other techniques to improve and optimize the yield has brought the possibility of the mass production of RES. More importantly, RES can be stabilized or protected by encapsulation, combined with other natural compounds, structurally modified and synthesized by chemo-enzymatic and other methods, which is of great significance for food, medicine, and clinical applications.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2025 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/8210896","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143111039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An Integrated Strategy for Discovering the Active Components and Pharmacological Mechanisms of Indigo Naturalis in Ameliorate Atherosclerosis Injury","authors":"Yuan-Pei Lian,&nbsp;Chun-Yan Yin,&nbsp;Jin-Yun Zhang,&nbsp;Yi Zhao,&nbsp;Ting Geng,&nbsp;Jian-Da Xu,&nbsp;Chan-Ming Liu,&nbsp;Jia-Li Cai,&nbsp;Li Zhu,&nbsp;Di-Jun Wang,&nbsp;Li Zhang,&nbsp;Li-Bo Luo,&nbsp;Xiao-Jing Yan","doi":"10.1155/jfbc/1159572","DOIUrl":"https://doi.org/10.1155/jfbc/1159572","url":null,"abstract":"<div>\u0000 <p>Atherosclerosis (AS), a chronic inflammatory vascular disease, is a major cause of cardiovascular morbidity and mortality worldwide. Indigo naturalis (IN) is a medicinal and edible plant with obvious pharmacological effects such as anti-inflammation, improving blood circulation, and antibacterial. However, its therapeutic efficacy and mechanism of action against AS remain unclear. This study aims to integrate network pharmacology, molecular docking, and <i>in vitro</i> and <i>in vitro</i> experimental evaluations to uncover the active components and multitarget mechanisms of IN against AS. 10 active ingredients and 96 related target genes were identified, and gene functional enrichment analysis suggested that the toll-like receptor signaling pathway plays a core role in IN’s action against AS. Molecular docking revealed strong binding affinities between IN’s key ingredients and hub genes. <i>In vitro</i>, IN regulated the expression of inflammatory factors, migration factors, and TLR4, NF-<i>κ</i>B, and MyD88 proteins in cell inflammation models as predicted. <i>In vivo</i>, IN reduced aorta damage, cell apoptosis, blood lipids level, and inflammatory factors in LPS-induced AS mice, further downregulating the expression levels of TLR4, NF-<i>κ</i>B, and MyD88. This study confirmed the feasibility of IN for AS treatment from an anti-inflammatory perspective, highlighting the TLR4/MyD88/NF-<i>κ</i>B pathway as a critical mechanism. These findings provide a basis for developing IN as a potential candidate for anti-AS clinical application.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2025 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/1159572","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143120977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Ganoderma lucidum Supplementation on Obesity and Metabolic Alterations Induced by High-Carbohydrate-High-Fat Diet in Rats","authors":"Farhana Lovely,&nbsp;Nazifa Sifat,&nbsp;S. M. Neamul Kabir Zihad,&nbsp;Ashit Kumar Dutta,&nbsp;Razina Rouf,&nbsp;Khondoker Shahin Ahmed,&nbsp;Md. Hemayet Hossain,&nbsp;Md. Golam Hossain,&nbsp;Jamil A. Shilpi,&nbsp;Shaikh J. Uddin","doi":"10.1155/jfbc/6894057","DOIUrl":"https://doi.org/10.1155/jfbc/6894057","url":null,"abstract":"<div>\u0000 <p><i>Ganoderma</i><i>lucidum</i> (GL), commonly called Reishi mushroom, has been utilized in traditional medicine against multiple ailments including obesity and metabolic complications. The purpose of this study was to evaluate the ability of the powdered supplementation of GL (2.5% w/w of diet) and its ability to ameliorate obesity and associated metabolic alterations in high-carbohydrate-high-fat (HCHF) diet–fed obese female Wistar rats. Thus, after 56 days of feeding the mice with different treatments, we measured physiological parameters to evaluate the effect. Phytochemical analysis by HPLC identified the bioactive polyphenols in GL. Results demonstrated that GL supplementation significantly (<i>p</i> &lt; 0.05) prevented the net rise in body weight and reduced organ weights and abdominal fat deposition in obese rats. It improved glucose intolerance, decreasing blood glucose level (6.2 nmol/L) compared to HCHF diet–induced obese rats (8.67 nmol/L). It also significantly ameliorated the plasma lipids parameters including total cholesterol (106.4 mg/dL), triglyceride, LDL-C (55.26 mg/dL), and VLDL-C, restored the elevated serum profiles of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP), and elevated the antioxidant enzymes including superoxide dismutase (SOD) (42.58 U/g tissue), catalase (CAT) activities, and glutathione (GSH) (62.63 nmol/g tissue) in obese rats. Furthermore, GL prevented hepatic fat infiltration in HCHF diet–fed rats preventing steatosis. Different bioactive polyphenols including catechin, rutin, cinnamic acid, vanillic acid, quercetin, ferulic acid, and kaempferol were detected in GL by HPLC analysis, which might be responsible for its activity. Bangladeshi cultivated GL possesses antiobesity effect and is rich in phenolics that could prevent obesity-associated metabolic disorders.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2025 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/6894057","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143120838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}