Journal of Food Biochemistry最新文献

{"title":"Rhamnetin-3-O-α-L-Rhamnoside Attenuates Alcoholic Liver Injury in Mice via Regulation of the Autophagy/Ferroptosis Pathway","authors":"Jiangtao Zhou,&nbsp;Borong Zhao,&nbsp;Fan Yang,&nbsp;Dandan Deng,&nbsp;Jiayu Chang,&nbsp;Lihan Yan,&nbsp;Hong Yang,&nbsp;Fei Yang,&nbsp;Liwen Han,&nbsp;Guan’e Yang","doi":"10.1155/jfbc/2599489","DOIUrl":"https://doi.org/10.1155/jfbc/2599489","url":null,"abstract":"<div>\u0000 <p><b>Background:</b> Rhamnetin-3-α-L-rhamnoside (ARR) is the principal monomer isolated from <i>Loranthus tanakae Franch</i>. and Sav, with the effect of prevention and treatment of inflammatory diseases. This study was to investigate the effects of ARR on acute alcoholic liver injury (ALI) in a mouse model and elucidate the mechanisms.</p>\u0000 <p><b>Methods:</b> Balb/c mice were divided into six groups: naive control (NC), model control (MC), positive control (PC), ARR low-dose group (ARR-L), medium-dose group (ARR-M), and high-dose group (ARR-H). The treatment lasted for 11 days, with alcohol administration on the 9th and 11th day. Blood samples were taken 24 h postadministration for glutamic pyruvic transaminase (ALT) and glutamic oxaloacetic transaminase (AST) detection. Liver tissue samples underwent hematoxylin–eosin (H&amp;E) staining, immunohistochemical analysis, and biochemical assays for glutathione (GSH), glutathione (MDA), superoxide dismutase (SOD), and tissue iron. Western blotting analysis was conducted for ferroptosis-related proteins including glutathione peroxidase 4 (GPX4), solute carrier family 7 member 11 (SLC7A11), and ferritin light chain (FTL) as well as autophagy-related proteins comprising benzyl chloride 1 (Beclin-1), autophagy-associated Gene 5 (ATG5), sequestosome 1 (P62), and microtubule associated protein 1 light chain 3 (LC3).</p>\u0000 <p><b>Results:</b> H&amp;E staining and analysis showed that alcohol caused liver cell damage, which was improved in the positive and ARR groups. The immunohistochemical analysis indicated decreased levels of heme oxygenase 1 (HO-1) and nuclear factor erythroid 2–related factor 2 (Nrf2) in the MC mice, whereas enhanced in the administration groups. Liver index, serum ALT, and AST significantly elevated in the MC group, which confirm successful modeling. Both positive drug and ARR ameliorated liver index, aminotransferase levels, oxidative stress, and tissue iron. Furthermore, western blotting revealed abnormal expression of ferroptosis- and autophagy-related proteins due to alcohol. In comparison with MC mice, administration groups showed significantly increased expressions of GPX4, SLC7A11, Beclin-1, ATG5, and LC3II/I and decreased expressions of FTL and P62.</p>\u0000 <p><b>Conclusion:</b> ARR prevents acute ALI by inhibiting ferroptosis and inducing autophagy. These findings suggested that ARR may represent a natural therapeutic approach for this disease.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2025 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/2599489","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143582041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combatting BPA-Induced Neurotoxicity With Purple Carrot Extract (Daucus carota): Modulation of Key Neurotransmitters and Cellular Pathways in Albino Rats","authors":"Amira M. El-Moslemany,&nbsp;Mai Hussein Abd-Elfatah,&nbsp;Wesam Mohammed Abd Elrahman,&nbsp;Manal Salah Abbas Elgendy,&nbsp;Heba I. Ghamry,&nbsp;Mohamed El-Sherbiny,&nbsp;Moaz Abdullah Mojaddidi,&nbsp;Lamya Ahmed Alkeridis,&nbsp;Nora Hamdy Mouhamed El-Wakeil,&nbsp;Mustafa Shukry","doi":"10.1155/jfbc/9071056","DOIUrl":"https://doi.org/10.1155/jfbc/9071056","url":null,"abstract":"<div>\u0000 <p><b>Ethnopharmacological Relevance:</b> Bisphenol A (BPA) is a widely used chemical, particularly in producing epoxy resins and polycarbonate plastics, which can contaminate food and water sources. Even low levels of BPA exposure have been associated with negative impacts on neural development, leading to neurotoxicity. Purple carrots, known for their health-promoting properties, are rich in bioactive compounds such as polyphenols.</p>\u0000 <p><b>Materials and Methods:</b> This study aimed to evaluate the neuroprotective effects of purple carrot extract in mitigating BPA-induced neurotoxicity in albino rats. Forty albino rats were divided into four groups: Group I (control) received water and a standard diet. Group II (BPA group) was orally administered 50 mg/kg body weight of BPA dissolved in 1 mL of olive oil daily for 30 days. Group III received 400 mg/kg of purple carrot extract along with 50 mg/kg of BPA daily. Group IV received 800 mg/kg of purple carrot extract along with 50 mg/kg of BPA daily, following the same 30-day oral administration protocol.</p>\u0000 <p><b>Results:</b> The results showed that treatment with purple carrot extract increased serum levels of critical neurotransmitters, including serotonin, dopamine, acetylcholine, acetylcholineesterase, and gamma-aminobutyric acid (GABA). Additionally, brain tissue analysis revealed elevated activity of antioxidant enzymes, such as catalase (CAT) and superoxide dismutase (SOD), in response to extract administration. Conversely, the extract significantly reduced levels of malondialdehyde (MDA), α-tumor necrosis factor (α-TNF), and interleukin-6 (IL-6) in brain tissues compared to the BPA-only group. These results indicate that purple carrot extract regulates genes related to oxidative stress, inflammation, and apoptosis in a dose-dependent manner during BPA-induced neurotoxicity. At a dose of 400 mg/kg, the extract increased the expression of antioxidant genes like Nrf2 and HO-1 while reducing the pro-apoptotic marker Bax. At the higher dose of 800 mg/kg, these protective effects were even more pronounced, leading to enhanced neuroprotection through increased antioxidant and antiapoptotic gene expression and reduced inflammation, mainly through the MAPK pathway.</p>\u0000 <p><b>Conclusion:</b> The study demonstrates that purple carrot extract reduces BPA-induced neurotoxicity in rats by enhancing antioxidants, neurotransmitters, and anti-inflammatory responses, suggesting natural protection against BPA-related neural damage.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2025 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/9071056","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143554435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ginger (Zingiber officinale Roscoe) Bioactive Components: Potential Resources for Kidney Health","authors":"Yichuan Wang,&nbsp;Yuehua Jiang,&nbsp;Cong Han,&nbsp;Le Zhou,&nbsp;Hongzhen Hu,&nbsp;Huihui Song,&nbsp;Wei Li","doi":"10.1155/jfbc/2625586","DOIUrl":"https://doi.org/10.1155/jfbc/2625586","url":null,"abstract":"<div>\u0000 <p>Ginger (<i>Zingiber officinale</i> Roscoe), being a customary dietary component across various regions globally, possesses numerous biological functionalities, such as antioxidative, anti-inflammatory, immune-regulatory, and anti-apoptotic properties. Ginger plays a crucial role in the management of kidney health due to its bioactive constituents, which are extensively implicated in various processes, including antioxidant activity, anti-inflammatory activity, amelioration of endothelial dysfunction, inhibition of cell apoptosis and necrosis, mitigation of renal fibrosis, and promotion of autophagy. In clinical trials, it was also found that the effect of ginger on blood sugar, blood lipid, and inflammatory factors could improve kidney function. These studies summarize the ginger active ingredients and their potential functions in the protection of kidney health, and provide an overview of the application and targeted delivery of ginger bioactive in functional food. In the future, more health-related information of bioactive compounds in ginger is needed, and their health effects for kidney should be further investigated.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2025 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/2625586","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143533455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microvegetables and Their Potential Health Relevance: A Systematic Review of In Vitro and In Vivo Evidence","authors":"Cealan O. Henry,&nbsp;Joanna L. Bowtell,&nbsp;Mary F. O’Leary","doi":"10.1155/jfbc/9953644","DOIUrl":"https://doi.org/10.1155/jfbc/9953644","url":null,"abstract":"<div>\u0000 <p>A rise in noncommunicable disease (NCD) prevalence has been partly attributed to the low intake of food groups rich in phytochemicals, particularly fruits and vegetables. For this reason, microvegetables (MV) are an emerging functional food group. Nutrient-dense with bioactive compounds, MV exhibit bioactive properties that may be relevant for NCD and malnutrition risk modulation. No chronic MV supplementation human clinical trials have been conducted. However, experimental in vitro and in vivo studies have shown that MV can exert a range of beneficial effects. In this systematic review, we analysed the effects of MV in such investigations and discussed the potential mechanisms involved. A bibliographical search was performed in PubMed and Scopus, and based on the inclusion criteria, 28 articles were summarised and critically discussed. Twelve studies demonstrated direct antioxidant capabilities of MVs via <i>in vitro</i> assays. Few studies investigated the more physiologically relevant impact of MV on endogenous cellular antioxidant systems. MV demonstrated proapoptotic activity in immortalised cell lines, with limited in vivo evidence indicating potential anti-inflammatory, lipid and gut microbiota-modifying effects. These properties warrant further investigation in human trials to evaluate their translational potential, and our summary of the literature offers a rationale and basis for such clinical trials.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2025 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/9953644","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143513752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shenbing Decoction III and Apigenin Improve Peritoneal Fibrosis Mediated by Epithelial–Mesenchymal Transition Through TAK1/p38MAPK/NF-κB Pathways","authors":"Yang Tang,&nbsp;Ying Wang,&nbsp;Xiaomin Sun,&nbsp;Jie Zheng,&nbsp;Jingying Fang,&nbsp;Shufeng Liu,&nbsp;Juan Luo,&nbsp;Yunqiang Liao,&nbsp;Yalin Cheng,&nbsp;Jiawei Lu,&nbsp;Shuyuan Zhou,&nbsp;Xiaosheng Li","doi":"10.1155/jfbc/7841565","DOIUrl":"https://doi.org/10.1155/jfbc/7841565","url":null,"abstract":"<div>\u0000 <p><b>Background:</b> This study aimed to evaluate the effect of Shenbing Decoction III (SBD III) and its key Apigenin on peritoneal dialysis (PD)–induced peritoneal fibrosis and explore the underlying mechanisms.</p>\u0000 <p><b>Methods:</b> Liquid chromatography–mass spectrometry confirmed the presence of Apigenin in SBD III. Human peritoneal mesothelial cells (HMrSV5) were stimulated with PD solution (PDS) to induce fibrosis, and treatment with SBD III and Apigenin was administered.</p>\u0000 <p><b>Results:</b> PDS decreased cell viability and increased the migration and invasion capabilities of HMrSV5 cells. It also reduced E-cadherin expression, while increasing the expression of α-SMA, fibronectin, collagen I, and TGF-β1. SBD III and Apigenin effectively counteracted these effects. Additionally, SBD III and Apigenin inhibited the PDS-induced upregulation and phosphorylation of transforming growth factor-β-activated kinase 1 (TAK1), p38, and nuclear factor-κB (NF-κB), similar to the effects of 5z-7-oxozeaenol. Chronic kidney disease (CKD) was induced in mice by 5/6 nephrectomy, followed by PDS treatment. The therapeutic effects of SBD III and Apigenin were evaluated. CKD mice exhibited reduced body weight and kidney weight indices, along with elevated blood urea nitrogen, urinary creatinine, and urinary protein levels. Fibrosis was observed in the kidneys and peritoneum. PDS treatment also significantly alleviated these effects. Notably, therapeutic effects were further augmented when SBD III and Apigenin were administered.</p>\u0000 <p><b>Conclusion:</b> SBD III and Apigenin ameliorated PDS-induced peritoneal fibrosis by inhibiting epithelial–mesenchymal transition via the TAK1/p38MAPK/NF-κB pathway, indicating promising pharmaceutical candidates for CKD.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2025 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/7841565","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143513751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of Commercial Preparations of Pectinases on the Chemical Composition and Stability of Phenolic Compounds in Grape Juices","authors":"Maria da Conceição Prudêncio Dutra,&nbsp;Ana Júlia de Brito Araújo Carvalho,&nbsp;Newton Carlos Santos,&nbsp;Sérgio Tonetto de Freitas,&nbsp;Ederlan de Souza Ferreira,&nbsp;Aline Telles Biasoto Marques,&nbsp;Marcos dos Santos Lima","doi":"10.1155/jfbc/2856691","DOIUrl":"https://doi.org/10.1155/jfbc/2856691","url":null,"abstract":"<div>\u0000 <p>Enzymatic preparations in juice processing come in different formulations, but their enzymatic activities on the stability of this beverage must be studied. This study evaluated five commercial yield and color extraction preparations concerning the chemical composition of grape juice, color, and phytochemicals quantified in HPLC. The activity of the enzymes was evaluated, and a study of the stability of the juices over 360 days was carried out. The color extraction enzymes Everzym Thermo and Endozym Rouge (hemicellulases) increased the content of flavonoids, especially malvidin-3-glucoside. The Endozym Pectofruit PR preparation (pectin lyase) stood out for its yield and extraction of organic acids such as tartaric. When assessing the stability of the juice using the half-life parameter, it became clear that all the juices showed similar evolutionary behavior, with more accelerated degradation of the components related to color in the first 100 days. Even though the Everzym Thermo preparation showed greater color extraction, it had higher degradation rates for these parameters and anthocyanins than the other preparations evaluated. At the end of the storage period, the differences in color and anthocyanins were small between the preparations, practically equal to the control. This shows that in the juice obtained without enzymes, the nondegradation of polysaccharides may be associated with a protective effect on color and anthocyanins.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2025 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/2856691","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143513689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Amelioratory Role of Lactobacillus paracasei N1115 on Blood Glucose, Inflammation, Cognitive Function, and Gut Microbiota Composition in Type 2 Diabetes Mellitus Rats","authors":"Qing Li,&nbsp;Feng Feng,&nbsp;Yanqing Bian,&nbsp;Hanyi Zhao,&nbsp;Sijia Cao,&nbsp;Dongzhe Sun,&nbsp;Tianyue Jiao,&nbsp;Baohua Zhao,&nbsp;Liping Peng","doi":"10.1155/jfbc/6891209","DOIUrl":"https://doi.org/10.1155/jfbc/6891209","url":null,"abstract":"<div>\u0000 <p>This study aims to investigate the effect of probiotic <i>Lactobacillus paracasei</i> N1115 on Type 2 diabetes mellitus (T2DM) rats. SD rats were randomly divided into 5 groups, including control group (ND), diabetes mellitus (DM) group, and high (10⁹ CFU/mL), medium (10⁸ CFU/mL), or low (10⁷ CFU/mL) doses of probiotics intervention groups. The ND rats were fed a normal diet, and the others were fed a high-fat diet and injected with streptozotocin (STZ) to build a T2DM model. In the three probiotic intervention groups, rats were given high, medium, or low doses of <i>L. paracasei</i> N1115 by gavage for 12 weeks. Results indicated that probiotics could prevent weight loss and decrease the blood glucose levels of T2DM rats, in a dose-dependent manner. A high dose of <i>L. paracasei</i> N1115 also led to a significant decline in the inflammatory response by regulating the TLR4/NF-κB inflammatory pathway and reducing proinflammatory cytokines, such as tumor necrosis factor-<i>α</i> (TNF-α), interleukin-1β (IL-1β), and lipopolysaccharide (LPS) (<i>p</i> &lt; 0.05). Besides, the memory factor cAMP and the cognitive regulatory pathway BDNF/TrkB were increased by high dose of <i>L. paracasei</i> N1115 treatment (<i>p</i> &lt; 0.05), revealing an enhanced cognitive memory competence. In addition, analysis of the gut microbiome showed that a high dose of <i>L.paracasei</i> N1115 decreased Firmicutes/Bacteroidota ratio and increased the relative abundance of beneficial bacteria, such as unclassified_Lachnospiraceae, Ligilactobacillus, and Lachnospiraceae_NK4A136_group. The microbial gene contents associated with metabolic pathways, nicotinate and nicotinamide metabolism, glycine, serine, and threonine metabolism and the citrate cycle were upregulated under <i>L. paracasei</i> N1115 treatment. In summary, this study elucidated the regulatory effects of <i>L. paracasei</i> N1115 on T2DM in rats and revealed a promising dietary supplement for the treatment of T2DM.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2025 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/6891209","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143513688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Antiobesity Mechanisms of Polygonatum cyrtonema Hua Based on Network Analysis and Experimental Validation In Vivo and In Vitro","authors":"Lihua Xing,&nbsp;Chenchen Zhao,&nbsp;Jun Xu,&nbsp;He Cheng,&nbsp;Jiayi Hu,&nbsp;Heng Liu,&nbsp;Mengjin Wang,&nbsp;An Zhou,&nbsp;Rongchun Han,&nbsp;Nianjun Yu","doi":"10.1155/jfbc/3379148","DOIUrl":"https://doi.org/10.1155/jfbc/3379148","url":null,"abstract":"<div>\u0000 <p>Obesity is a chronic and complex disease that serves as a precursor to numerous chronic conditions. Its development is linked to gut microbiota and often accompanies inflammation and insulin resistance. The rhizome of <i>Polygonatum cyrtonema</i> Hua is commonly employed in the treatment of metabolic disorders. The mechanisms of <i>P</i>. <i>cyrtonema</i> Hua in treating obesity have not been deeply investigated. This study aimed to explore the protective effect and the possible mechanism of the water extract of <i>P</i>. <i>cyrtonema</i> Hua (PHWE) in high-fat diet–induced obesity in mice. We employed a combination of network pharmacology and molecular docking methods to predict the mechanisms of traditional Chinese medicine in treating obesity, followed by <i>in vivo</i> and <i>in vitro</i> validations. After a series of biological information analyses, 10 potential core targets were identified: AKT1, PPARG, ESR1, STAT3, SIRT1, PPARA, MMP9, FASN, mTOR, and IGF1R. The key pathways mainly include the AMPK signaling pathway, insulin signaling pathway, insulin resistance, and mTOR signaling pathway. In experiments conducted on live mice with obesity, PHWE was found to significantly reduce body weight, IL-6, and IL-1β levels in adipose tissue and improve insulin resistance. Based on the characteristics of obesity, the modulation of inflammation and insulin resistance by PHWE in obese mice may be related to gut microbiota. PHWE modulated the mTOR signaling pathway by decreasing the expression of mTOR, Raptor-mTORC1, and pS6K1 proteins while increasing the expression of pIRS1 and pAKT. PHWE could also regulate the protein expression of PPARγ and the key target FASN. These findings were further validated in <i>in vitro</i> experiments. Combining network pharmacology with <i>in vivo</i> and <i>in vitro</i> experiments, we investigated the potential mechanisms of PHWE in treating obesity. These findings provide an experimental foundation for the clinical utilization of <i>P</i>. <i>cyrtonema</i> Hua.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2025 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/3379148","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143513702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation, Identification, and Cellular Antioxidant Activity Evaluation of Antioxidant Peptides From the Antler Tips of Sika Deer (Cervus nippon)","authors":"Xingyu Xiao,&nbsp;Jilun Meng,&nbsp;Xi Chen,&nbsp;Yi Li,&nbsp;Shuo Zheng,&nbsp;Xunming Zhang,&nbsp;Libo Zhang,&nbsp;Jiayuan Fang,&nbsp;Tong Su,&nbsp;Ze Ma,&nbsp;Hong Zhang,&nbsp;Linlin Hao","doi":"10.1155/jfbc/9974213","DOIUrl":"https://doi.org/10.1155/jfbc/9974213","url":null,"abstract":"<div>\u0000 <p>A process for extracting the antler tip peptides (ATPs) from Sika deer (<i>Cervus nippon</i>) antler tips was optimized using a method that combines single-factor experiment and response surface methodology. The optimal conditions involved papain as the hydrolase, an enzyme concentration of 5000 U/g, a hydrolysis time of 3.0 h, a temperature of 52.0°C, a pH of 7.10, and a substrate concentration of 14%. Under these conditions, the clearance rate of 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical was 73.05 ± 1.49%, and the degree of hydrolysis (DH) was 19.43 ± 1.06%. The antioxidant compound ATPs-2 exhibited strong free radical and anion scavenging activity. In a model of Ultraviolet B (UVB)-induced oxidative damage, ATP-2 reduced reactive oxygen species (ROS) and malondialdehyde (MDA) levels, increased superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) activities, and alleviated UVB-induced cellular oxidative damage. A total of 6279 unique peptide sequences were identified in ATP-2, with 2496 potential bioactive peptides predicted using PeptideRanker. Of these, 12 peptides with PeptideRanker scores above 0.95 were analyzed using BIOPEP-UWM, identifying three antioxidant peptides: GPWPPGAP, GPAGPPGGPGL, and PGSPGPPGMPGP. Among them, GPWPPGAP displayed the highest antioxidant activity, with an IC₅₀ value on DPPH radical of 1.1 mg/mL and a strong binding energy of −9.8 kcal/mol to MMP-1.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2025 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/9974213","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143497116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structurally Diverse Quinoline Alkaloids With Antileukaemia Activities From Taraxacum mongolicum","authors":"Xiaodong Zhang,&nbsp;Qinge Ma,&nbsp;Weiwei Yan,&nbsp;Meng Li,&nbsp;Haixia Liu,&nbsp;Bo Yuan,&nbsp;Xiaoxue Wang","doi":"10.1155/jfbc/5545155","DOIUrl":"https://doi.org/10.1155/jfbc/5545155","url":null,"abstract":"<div>\u0000 <p><i>Taraxacum mongolicum</i>, a medicinal and food homologous traditional Chinese medicine, has important research value in clinical practice. Eight new quinoline alkaloids (<b>1</b>–<b>8</b>), including 1N-geranyl quinoline alkaloids (<b>1</b>–<b>3</b>), 3,4-phenylquinoline alkaloids (<b>4</b>–<b>6</b>) and 10-carbonyl-11-pyrrolyl quinoline alkaloids (<b>7</b>–<b>8)</b>, and twelve known quinoline alkaloid derivatives (<b>9</b>–<b>20</b>) were isolated from <i>T. mongolicum</i> for the first time. These compounds were elucidated according to analysing NMR, MS, UV and IR spectra and compared with references. Among them, compounds (<b>7</b>, <b>8</b>, <b>14</b> and <b>16</b>) showed certain antileukaemia activities, which exhibited important cell growth inhibitory activities against HL-60 and K562 cells than adriamycin, which could be potential antileukaemia agents. The plausible biogenetic pathway and preliminary structure–activity relationship of the selected compounds were scientifically summarised and discussed in this work.</p>\u0000 </div>","PeriodicalId":15802,"journal":{"name":"Journal of Food Biochemistry","volume":"2025 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jfbc/5545155","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143481582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}