Iranian journal of veterinary research最新文献

{"title":"Respiratory and intestinal zoonotic cryptosporidiosis in symptomatic domestic pigeons (<i>Columba livia domestica</i>) in Tabriz, Iran.","authors":"M Khordadmehr, A Sazmand, A Oryan, P Almasi, V Ranjbar, A Hemphill, D Otranto","doi":"10.22099/ijvr.2024.49762.7335","DOIUrl":"10.22099/ijvr.2024.49762.7335","url":null,"abstract":"<p><strong>Background: </strong>Domestic pigeons (Columba livia domestica<i>)</i> are the oldest domesticated birds worldwide, harboring many zoonotic parasites and posing potential public health threats.</p><p><strong>Aims: </strong>To investigate cryptosporidiosis in domestic pigeons in Tabriz, Iran, 100 privately owned pigeons presenting weight loss and diarrhea were tested for Cryptosporidium spp. through parasitological, histopathological, and molecular tests.</p><p><strong>Methods: </strong>Modified Ziehl-Neelsen-stained fecal smears and histological sections of the trachea and small intestine were examined microscopically. Genomic DNA of fecal and tracheal specimens was examined by nested conventional PCR targeting <i>18S rDNA</i>, followed by Sanger sequencing of histopathology-confirmed samples and phylogenetic analyses.</p><p><strong>Results: </strong>All pigeons were positive at PCR in their feces and trachea. Oocysts similar to the size of Cryptosporidium species were observed in stained fecal smears of 62% of pigeons. At the histopathological examination, Cryptosporidium<i>-</i>organisms were observed on the apical epithelial surfaces of the small intestine in 84% and trachea in 78% of pigeons. In 23 pigeons, simultaneous tracheal and intestinal cryptosporidiosis was determined. The lesions in affected tracheas and small intestines included hyperemia, villous atrophy and fusion, dilatation of intestinal crypts, irregular epithelial hyperplasia, and sloughing. Diffused mixed inflammatory cell infiltration in the lamina propria was observed, with dominant lymphocytes, plasma cells, and lower numbers of heterophils. Consensus sequences of detected parasites revealed infection with Cryptosporidium parvum and Cryptosporidium meleagridis.</p><p><strong>Conclusion: </strong>Considering the high frequency of cryptosporidiosis reported here in symptomatic birds and that both identified Cryptosporidium species are zoonotic parasites, findings claim a public health risk assessment of this species of animals.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"25 4","pages":"326-332"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12085204/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144093670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing the shelf life of rainbow trout fillets using edible sodium caseinate coating with <i>Oliveria decumbens</i> essential oil nanoemulsion, vitamin E and sodium dodecyl sulfate.","authors":"L Nikravan, S Maktabi, M Ghaderi Ghahfarrokhi, M Mahmoodi Sourestani","doi":"10.22099/ijvr.2024.48884.7185","DOIUrl":"10.22099/ijvr.2024.48884.7185","url":null,"abstract":"<p><strong>Background: </strong>The use of essential oil nanoemulsions has emerged as a promising strategy for extending the shelf life of highly perishable foods, like fish, by leveraging their natural antimicrobial and antioxidant properties.</p><p><strong>Aims: </strong>This study investigated the effects of an edible sodium caseinate coating incorporating <i>Oliveria decumbens</i> essential oil (<i>Od</i>-EO) and its nanoemulsion (<i>Od</i>-NEO), along with vitamin E and sodium dodecyl sulfate (SDS), on the shelf life of rainbow trout fillets during refrigeration.</p><p><strong>Methods: </strong>Fish samples were coated by immersion in pure sodium caseinate (SC), SC+<i>Od</i>-EO, and SC+<i>Od</i>-NEO. Sensory (odor, color, texture, overall acceptance), chemical (pH, total volatile basic nitrogen [TVB-N], thiobarbituric acid [TBA], and peroxide value [PV]), and microbiological analyses (total mesophilic aerobic bacteria, psychrophilic anaerobic bacteria, and <i>Pseudomonas</i> spp. counts) were conducted over 15 days of storage at 4°C.</p><p><strong>Results: </strong>The study results indicated that fillets treated with SC+<i>Od</i>-NEO exhibited significantly higher quality compared with other treatments. Additionally, the combination of nanoemulsion, vitamin E, and SDS showed synergistic effects in reducing microbial growth, delaying lipid oxidation, and improving organoleptic quality, extending the shelf life of rainbow trout fillets up to 15 days. Overall, the correlation analysis highlights the interdependence of microbial, chemical, and sensory parameters and reinforces the role of SC-NEO-based treatments in mitigating spoilage and maintaining the sensory quality of fish fillets during storage.</p><p><strong>Conclusion: </strong>The results highlight the potential of nanoemulsion-based coatings as an innovative and effective strategy for extending the shelf life of highly perishable fish products. Coting with SC+<i>Od</i>-NEO+Vit E+SDS can be recommended as an effective food preservative.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"25 4","pages":"333-343"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12085207/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144093599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of topical NPH-insulin use on wound healing and oxidative stress in alkali corneal burns: an experimental study in a rabbit model.","authors":"B Kurt, L Balyen, O Atakişi, S Yiğit, U Aydin, I Özaydin","doi":"10.22099/ijvr.2024.47545.6867","DOIUrl":"10.22099/ijvr.2024.47545.6867","url":null,"abstract":"<p><strong>Background: </strong>In acute alkali corneal burns, the routine treatment protocol includes suppressing inflammation using corticosteroids. In recent years, there has been a growing interest in the role of insulin in wound healing research due to its remarkable positive effects.</p><p><strong>Aims: </strong>The present study aimed to compare the healing effects of dexamethasone -the conventional approach- and NPH-insulin on alkali corneal burns.</p><p><strong>Methods: </strong>An alkali corneal burn was created using Ca (OH)₂ in all subjects. One group (n=6) was treated with an ophthalmic ointment containing dexamethasone (group I), and another group (n=6) was treated with an insulin ointment (group II). The control group (n=6) received no treatment (group III). Clinical changes in the corneal burn areas were monitored on days 3, 7, 14, and 21. The animals were euthanized on day 21. The excised corneas were examined for histopathological, immunohistochemical, and biochemical changes.</p><p><strong>Results: </strong>A more successful clinical recovery graph was drawn for group II. Particularly, a significant (P<0.05) improvement was detected in group II on day 21. The highest positivity in MMP-9 corneal staining was found in group I. Group II had a significant (P<0.05) increase in total antioxidant capacity (TAC) values. The treatment groups showed a significant (P<0.05) decrease in total oxidant content (TOC) values and a significant (P<0.05) increase in reduced glutathione (GSH) levels as compared with the control group.</p><p><strong>Conclusion: </strong>Topical NPH-insulin provided rapid and uncomplicated clinical recovery of alkali corneal burns. Insulin and dexamethasone showed similar effects like increased antioxidant molecules and decreased oxidant substances which indicated that insulin may prevent free radical formation in the cornea.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"25 3","pages":"202-209"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11801317/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143382412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of <i>Brucella</i> infection in raw milk of livestock in Famenin, West part of Iran.","authors":"M Adabi, J Gharekhani, S Alamian, M Varasteh Shams, M Fathi Sheikh, Z Shivapour, Z Valiei, S Khazaiee, H Ghaderi","doi":"10.22099/IJVR.2024.49557.7292","DOIUrl":"https://doi.org/10.22099/IJVR.2024.49557.7292","url":null,"abstract":"<p><strong>Background: </strong>Timely diagnosis of brucellosis is the starting point for effective programs to control brucellosis in humans and animals. <b>Aims:</b> This study aimed to detect <i>Brucella</i> infection in milk samples from livestock in Famenin, an endemic region of western Iran, using a milk ring test and molecular techniques.</p><p><strong>Methods: </strong>In this cross-sectional study, 738 raw milk samples were randomly collected from cattle, sheep, and goats. Milk samples were screened using the milk ring test (MRT). In addition, polymerase chain reaction (PCR) method was applied to detect <i>Brucella</i> spp. in all MRT-positive samples. DNA from the milk samples was extracted and used for PCR using the <i>BCSP31</i> and <i>IS711</i> loci.</p><p><strong>Results: </strong>Of the samples tested using MRT, 46 (6.23%, 95% CI: 2.83-9.63%) yielded positive results. Of the 46 seropositive samples, 42 (91.30%) were from sheep and 4 (8.70%) were from goats, while no bovine samples had positive MRT results. PCR analysis confirmed that 78.26% (36/46) of MRT-positive samples belonged to the genus <i>Brucella</i>. Furthermore, 83.33% (30/36) of the confirmed samples were identified as <i>B. melitensis</i>, while 16.66% (6/36) were identified as <i>B. abortus</i>.</p><p><strong>Conclusion: </strong>The results obtained from MRT evaluation of milk samples did not align entirely with the findings of the molecular examinations. The PCR method has minimal biological contamination and high sensitivity and accuracy, especially for determining <i>Brucella</i> species. Raw milk should be routinely assessed for <i>Brucella</i> contamination. This work is necessary to identify hidden infections and break the chain of transmission of brucellosis.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"25 2","pages":"156-160"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11608534/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142768910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Whole genome sequencing analysis of non-O157 Shiga toxin-producing <i>Escherichia coli</i> in milk in Kwara State, Nigeria.","authors":"I Ghali-Mohammed, I A Odetokun, I A Raufu, V O Adetunji","doi":"10.22099/IJVR.2024.49643.7307","DOIUrl":"https://doi.org/10.22099/IJVR.2024.49643.7307","url":null,"abstract":"<p><strong>Background: </strong><i>Escherichia coli</i> is a major cause of poor microbial quality of milk, often resulting from unhygienic milk handling. Milk contamination poses public health concerns. Shiga toxin-producing <i>Escherichia coli</i> (STEC) strains in food products, particularly milk, is a critical concern for public health. Limited information exists on the detection of non-O157 <i>E. coli</i> pathotypes in milk sold by local processors in Nigeria.</p><p><strong>Aims: </strong>This study aimed to explore the diversity of non-O157 STEC isolates found in commercially available milk in Kwara State, Nigeria, to find the genetic diversity and potential risks associated with these strains.</p><p><strong>Methods: </strong>A subgroup of 18 representative non-O157 STEC isolated from milk samples (n=1225) was selected for whole genome sequencing (WGS) analysis.</p><p><strong>Results: </strong>Four novel sequence types (ST): ST398, ST540, ST1727, and ST9891 of non-O157 <i>E. coli</i> involving five serotypes: O176:H30, O176:H20, O8:H20, O21:H45, and O22:H7, carrying variable proportions of virulence factors, antimicrobial resistance genes, and plasmids, were identified.</p><p><strong>Conclusion: </strong>This investigation contributes valuable data to the ongoing efforts to ensure food safety and prevent the transmission of <i>E. coli</i> strains through dairy products. The findings have implications for public health policies and food quality standards in Kwara State, Nigeria. Improved hygienic practices during milk handling are recommended.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"25 2","pages":"92-97"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11608529/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142768967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phenotypic and genotypic detection of multi drug resistant coagulase-positive <i>Staphylococcus</i> spp. isolates from canine pyoderma.","authors":"K Shobha, S N Ghodasara, D B Barad, B B Javia, P J Poshiya, D K Parasana","doi":"10.22099/IJVR.2024.49531.7283","DOIUrl":"https://doi.org/10.22099/IJVR.2024.49531.7283","url":null,"abstract":"<p><strong>Background: </strong>Dermatological infections in dogs are challenging to treat due to antibiotic resistance, which leads to longer recovery time and the need for stronger antibiotics. <b>Aims:</b> This study was undertaken to determine the prevalence of antimicrobial resistance in coagulase-positive staphylococcal isolates from pyoderma infection in dogs. This study also aimed to identify isolates with methicillin-resistance and multidrug resistance.</p><p><strong>Methods: </strong>73 coagulase-positive staphylococci isolated from varying degrees of canine pyoderma cases. The samples were analyzed for the presence of <i>Staphylococcus</i> spp. using polymerase chain reaction (PCR) and resistance against antibiotics was studied by antimicrobial profile, minimum inhibitory concentration (MIC), and PCR on isolated bacteria.</p><p><strong>Results: </strong>Out of 75 bacterial isolates identified, 73 isolates were confirmed as <i>Staphylococcus</i> species by PCR. A higher percentage of antibiotic resistance was observed against penicillin-G (46.27%), followed by amoxiclav (38.81%), enrofloxacin (32.84%), cefpodoxime, oxytetracycline (28.36% each), levofloxacin (26.86%), and co-trimoxazole (22.39%). 29 (49.15%) S. pseudintermedius<i>,</i> three (50.00%) S. schleiferi subsp. coagulans<i>,</i> and two (100%) S. aureus isolates exhibited multidrug resistance. However, one (1.49%) isolate (S. pseudintermedius) revealed low-level mupirocin resistance in the E-test. Also, 12 (20.34%) methicillin-resistant Staphylococcus pseudintermedius (MRSP), one (16.67%) methicillin-resistant S. schleiferi subsp. coagulans (MRSS) and one (50%) methicillin-resistant S. aureus (MRSA) were reported using PCR.</p><p><strong>Conclusion: </strong>This study helps to understand the increased level and pattern of resistance in coagulase-positive staphylococci isolated from different types of canine pyoderma cases.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"25 2","pages":"117-124"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11608525/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142768915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrasonographic characterization of tendons and ligaments of palmar/plantar aspect of the cannon region in Egyptian donkeys.","authors":"S Gadallah, A Sharshar, M Fadel, E Mahran, A Hammad","doi":"10.22099/IJVR.2024.47480.6859","DOIUrl":"https://doi.org/10.22099/IJVR.2024.47480.6859","url":null,"abstract":"<p><strong>Background: </strong>There is a scarcity of data regarding the ultrasonographic characterization of tendons and ligaments in the distal limbs of donkeys.</p><p><strong>Aims: </strong>To determine ultrasonographic characteristics of normal tendons and ligaments at the palmar/plantar aspect of the cannon region in Egyptian donkeys.</p><p><strong>Methods: </strong>B-mode ultrasonography was conducted for the proposed tendons and ligaments in 12 clinically normal donkeys. Targeted structures were examined using transverse and longitudinal scans and evaluated in shape, echogenicity, echogenic pattern, fiber alignment pattern, and cross-sectional area (CSA).</p><p><strong>Results: </strong>Using transverse scan, the sonographic shapes of tendons and ligaments of metacarpal and metatarsal regions were determined. Upon examining different levels of each region, specific ligaments, not tendons, were present only at the higher levels. The echogenicity of ligaments and tendons was either similar or variable across different levels. All tendons and ligaments displayed homogeneous echogenicity except for the suspensory ligament. In the longitudinal scan, tendons maintained linear and parallel fiber alignment along examination levels. Conversely, ligaments displayed mostly inconstant fiber patterns (linear/crimp). There was a statistically significant (P<0.05) difference in CSA of tendons and ligaments between certain levels within metacarpal and metatarsal regions. Upon comparing metacarpal and metatarsal regions, there were distinct variations in some ultrasonographic characteristics of the same tendons and ligaments.</p><p><strong>Conclusion: </strong>This study established the ultrasonographic features of normal tendons and ligaments at the palmar/plantar aspect of the cannon region in donkeys (<i>Equus asinus</i>). These ultrasonographic data can be a reference guide when cannon region lameness is suspected.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"25 2","pages":"143-155"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11608532/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142768962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of major OMPs and LPS of <i>Brucella</i> on the control of activation of bone marrow-derived dendritic cells and proliferation of T-lymphocytes in mice.","authors":"Z Xu, Z Tong, H Zhang, X Deng, N Yang, Z Wang, C Chen","doi":"10.22099/ijvr.2024.48969.7177","DOIUrl":"10.22099/ijvr.2024.48969.7177","url":null,"abstract":"<p><strong>Background: </strong><i>Brucella</i> outer membrane proteins (OMPs) are highly immunogenic, and lipopolysaccharides (LPS) are also considered significant antigens, making them potential candidates for subunit vaccines.</p><p><strong>Aims: </strong>To investigate the effects of <i>Brucella</i> OMPs and LPSs on mouse bone marrow-derived dendritic cells (BMDC) activation and T-lymphocyte proliferation.</p><p><strong>Methods: </strong>BMDC were isolated and cultured <i>in vitro</i>, and subsequently co-cultured with <i>Brucella</i> recombinant proteins (rOMP10, rOMP19, rBP26, rOMP25, and rOMP31), as well as smooth LPS (S-LPS) or rough LPS (R-LPS). The expression of maturation markers on the surface of BMDCs was determined using flow cytometry, while the expression of TLR receptors was determined using RT-PCR. The levels of inflammatory cytokines were measured using iELISA, and the impact on the proliferation of mouse T-lymphocytes was assessed using the MTT method.</p><p><strong>Results: </strong>The impact of LPS on BMDC maturation, TLRs-mediated cytokine secretion, and antigen presentation was found to be limited. In contrast, rOMP10, rOMP19, and rBP26 were observed to promote BMDC maturation, increase the expression of TLR-2 and TLR-4 mRNA, and activate T-lymphocyte proliferation by significantly increasing the expression of pro-inflammatory cytokines (TNF-α, IFN-γ, IL-6, IL-12) and antigen-presenting molecules. However, rOMP25 and rOMP31 did not promote BMDC maturation, inhibited the expression of MHCI and MHCII antigen-presenting molecules, and increased the expression of inflammation-suppressing cytokines (IL-10 and IL-4), resulting in the inhibition of T-lymphocyte proliferation.</p><p><strong>Conclusion: </strong><i>Brucella</i> OMP10, OMP19, and BP26 play an important role in activating the host immune response, while OMP25 together with OMP31 may play a role in <i>Brucella</i> immune escape.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"25 3","pages":"224-232"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11801326/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143382371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative effects of herbal additive, symbiotic and antibiotic on growth performance, blood constituents, gut microbiota, and immune response in broiler chickens.","authors":"F Tavakolinasab, K Taherpour, A Rostamzad","doi":"10.22099/ijvr.2024.49567.7298","DOIUrl":"10.22099/ijvr.2024.49567.7298","url":null,"abstract":"<p><strong>Background: </strong>Using medicinal plants in broiler diets has been gaining attention as an alternative to synthetic additives due to their potential health benefits and lower risk of residue accumulation.</p><p><strong>Aims: </strong>The present study primarily aimed to evaluate and compare the effects of herbal additives, specifically barberry (<i>Berberis vulgaris</i>), sumac (<i>Rhus coriaria</i>, L), symbiotic, and antibiotic on broiler chickens.</p><p><strong>Methods: </strong>A total of 384 one-day-old broiler chicks (Ross 308) were assigned to eight different dietary treatments, with six replicates per treatment. Experimental diets included control diet (CON), and other experimental groups were supplemented with 0.2 g/kg virginiamycin (VM), symbiotic (SS), 1 g/kg (B₁) and 2 g/kg (B₂) barberry seed powder, 1 g/kg (S₁) and 2 g/kg (S₂) sumac seed powder and 1 g/kg sumac seed powder + 1 g/kg barberry seed powder (B₁ + S₁).</p><p><strong>Results: </strong>The study results indicated that body weight increased in birds fed with VM and SS-supplemented diets (P<0.05) during 1 to 42 days of age. All dietary treatments except CON increased the count of <i>Lactobacillus</i> spp. and decreased the number of coliforms versus at the end of the experiment (P<0.05). Antibody titers against Gambaro disease were higher in birds fed diets B2 and B1 + S1 (P<0.05). The results also indicated that the heterophil to lymphocyte ratio was significantly lower in the SS and B1 + S1 groups as compared with the control group.</p><p><strong>Conclusion: </strong>A mix of sumac and barberry (1%) powdered seeds has the potential to improve performance, and disease responsiveness and intestinal microbiota in broiler.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"25 3","pages":"242-249"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11801321/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143382219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cloning and expression of Fusion and Hemagglutinin proteins of peste des petits ruminants virus in the baculovirus system: an immunogenicity study in mice.","authors":"M Taheri Asl, M Moghbeli, M Kargar, M Lotfi, F Kafilzadeh","doi":"10.22099/ijvr.2024.48368.7047","DOIUrl":"10.22099/ijvr.2024.48368.7047","url":null,"abstract":"<p><strong>Background: </strong><i>Peste des petits ruminants virus</i> (<i>PPRV</i>) is one of the most economically important pathogens in sheep and goats. Fusion (F) and Hemagglutinin (H) proteins are the main immune-stimulating antigens.</p><p><strong>Aims: </strong>The present study aimed to clone and express F and H genes in baculovirus, and evaluate the immunogenicity of recombinant proteins produced by sf9 cells in mice.</p><p><strong>Methods: </strong>Amplified F and H genes (by RT-PCR using specific primers) were cloned into pFastBac Dual plasmid. The recombinant plasmid was transformed in DH10Bac host cells. SDS-PAGE and Western blotting was performed to control the recombinant protein, and a whole pure and specific recombinant protein was obtained.</p><p><strong>Results: </strong>The immunogenicity of 20 μg of non-adjuvant recombinant proteins in Balb-c mice showed better results compared with the attenuated PPR vaccine.</p><p><strong>Conclusion: </strong>The recombinant protein obtained from this study can be a suitable candidate for the production of recombinant vaccines against PPRV.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"25 3","pages":"210-215"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11801324/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143382259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}