Cloning and expression of Fusion and Hemagglutinin proteins of peste des petits ruminants virus in the baculovirus system: an immunogenicity study in mice.

IF 0.8 4区农林科学 Q3 VETERINARY SCIENCES

Iranian journal of veterinary research Pub Date : 2024-01-01 DOI:10.22099/ijvr.2024.48368.7047

M Taheri Asl, M Moghbeli, M Kargar, M Lotfi, F Kafilzadeh

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引用次数: 0

Abstract

Background: Peste des petits ruminants virus (PPRV) is one of the most economically important pathogens in sheep and goats. Fusion (F) and Hemagglutinin (H) proteins are the main immune-stimulating antigens.

Aims: The present study aimed to clone and express F and H genes in baculovirus, and evaluate the immunogenicity of recombinant proteins produced by sf9 cells in mice.

Methods: Amplified F and H genes (by RT-PCR using specific primers) were cloned into pFastBac Dual plasmid. The recombinant plasmid was transformed in DH10Bac host cells. SDS-PAGE and Western blotting was performed to control the recombinant protein, and a whole pure and specific recombinant protein was obtained.

Results: The immunogenicity of 20 μg of non-adjuvant recombinant proteins in Balb-c mice showed better results compared with the attenuated PPR vaccine.

Conclusion: The recombinant protein obtained from this study can be a suitable candidate for the production of recombinant vaccines against PPRV.

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来源期刊

Iranian journal of veterinary research 农林科学-兽医学

CiteScore

1.70

自引率

8.30%

发文量

期刊介绍： The Iranian Journal of Veterinary Research(IJVR) is published quarterly in 4 issues. The aims of this journal are to improve and expand knowledge in all veterinary fields. It is an international journal indexed by the Thomson Institute for Scientific Information (ISI), Elsevier, Scopus, CAB International, Veterinary Bulletin and several other international databases. Research papers and reports on a wide range of veterinary topics are published in the journal after being evaluated by expert reviewers.The Editor-in-Chief is responsible for the editorial content of the journal—including peer-reviewed manuscripts—and the timing of its publication.