Iranian journal of veterinary research最新文献

{"title":"Mechanisms in colistin-resistant superbugs transmissible from veterinary, livestock and animal food products to humans.","authors":"N Satarzadeh, A Saraee, Z Hatif Mahdi, A Sadeghi Dousari, M Armanpour, M Taati Moghadam","doi":"10.22099/ijvr.2024.50497.7453","DOIUrl":"10.22099/ijvr.2024.50497.7453","url":null,"abstract":"<p><p>In the era of antibiotic resistance, where multidrug-resistant (MDR), extensively drug resistant (XDR), and pan-drug resistant (PDR) Gram-negative infections are prevalent, it is crucial to identify the primary sources of antibiotic resistance, understand resistant mechanisms, and develop strategies to combat these mechanisms. The emergence of resistance to last-resort antibiotics like colistin has sparked a war between humanity and resistant bacteria, leaving humanity struggling to find effective countermeasures. Although colistin is used as a highly toxic antibiotic in infections that are not treated with routine antibiotics, its widespread use in animal breeding and veterinary medicine has contributed to the spread of colistin-resistant bacteria, plasmid-borne colistin resistance genes (<i>mcr</i>), and antibiotic residues in livestock and animal-derived foods. These sources can potentially transmit colistin resistance to humans through various routes. Therefore, managing the use of colistin in livestock and animal foods, implementing strict monitoring, and establishing guidelines for its proper use are essential to prevent the escalation of colistin resistance. This review article discusses the latest mechanisms of colistin antibiotic resistance, particularly biofilm production as a public health threat, the livestock and animal food sources of this resistance, and the routes of transmission to humans.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"25 4","pages":"298-311"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12085205/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144093663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MHC-linked microsatellite LEI0258 variability and population structure of chicken ecotypes in Iran.","authors":"A Ramezani, G Nikbakht Brujeni, N Sheikhi, K Parvandar Asadollahi","doi":"10.22099/ijvr.2024.50968.7544","DOIUrl":"10.22099/ijvr.2024.50968.7544","url":null,"abstract":"<p><strong>Background: </strong>Investigation of chicken major histocompatibility complex (MHC) genes along with their diversity across native regions and populations, as a genetic resource, can be used in the breeding programs. Important characteristics of MHC genes, such as the association with immunological and production traits, make them exceptional candidates for marker assisted selection.</p><p><strong>Aims: </strong>The aim of this study was to characterize MHC based on the LEI0258 microsatellite marker to evaluate the genetic variability and diversity within and between Iranian chicken populations.</p><p><strong>Methods: </strong>Blood samples were collected from six indigenous ecotypes (n=633) and Ross 308 (n=216) as a commercial breed. The MHC variability was determined based on a microsatellite marker located within MHC, LEI0258. Polymerase chain reaction and fragment analysis was used for microsatellite polymorphism detection.</p><p><strong>Results: </strong>Based on the fragment analysis, 7 alleles were found in Ross 308 and 25 alleles across all 6 populations. The population with the maximum genetic diversity was Mazandaran (0.939), while the population with the minimum genetic diversity was Ross 308 (0.794). Out of the 6 Iranian chicken ecotypes, all except Arian and Khorasan, were in Hardy-Weinberg equilibrium (P<0.05). The genetic variations within (84.92%) and between (15.08%) populations were statistically significant (P<0.001).</p><p><strong>Conclusion: </strong>A significant genetic structure that is not completely homogeneous among the Iranian chicken populations can be considered as distinct genetic resources. This study highlights the value of using markers such as LEI0258 to investigate the diversity of genes that play dual roles in immunity and production.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"25 4","pages":"312-318"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12085208/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144093664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effects of music and darkness on radionuclide distribution during mice FDG-PET scan.","authors":"K Tanha, M Seyedabadi, H Ghadiri, M Ay","doi":"10.22099/ijvr.2024.49641.7306","DOIUrl":"10.22099/ijvr.2024.49641.7306","url":null,"abstract":"<p><strong>Background: </strong>There is growing interest in the therapeutic potential of music or light in different human disorders.</p><p><strong>Aims: </strong>This study aimed to evaluate the effects of music as well as darkness on FDG uptake in 4T1 tumor-bearing BALB/c mice using a PET scan.</p><p><strong>Methods: </strong>The music, darkness, and music plus darkness groups were subjected to either song or darkness and their combination, respectively, 30 min before the radiopharmaceutical injection until the end of the experiments. The control group was imaged in silence under ambient conditions.</p><p><strong>Results: </strong>Our results revealed that music did not significantly alter the range of tumor SUVmean, but showed a slight increase in brain SUVmean (18.2%) and about 100% increase in brain percentage of injected dose per gram (%ID/g) in <i>ex vivo</i> analysis. In contrast, heart SUVmean and heart %ID/g were approximately half those of the silence group. The muscle SUVmean and blood activity measurements showed a decrement upon music exposure. Also, results showed a significant difference in tumor-to-muscle ratio (85% increment) and brain-to-muscle ratio (105% increment) between the silence and music groups. The muscle SUVmean decreased by 50%, and tumor-to-muscle and brain-to-muscle ratios were observed to increase by 44% and 60% in the group exposed to darkness, respectively.</p><p><strong>Conclusion: </strong>Our results suggest that music and environmental factors may influence FDG uptake in small-animal PET imaging, and provide important insights into the reliability of FDG-PET imaging for music intervention research and may aid researchers in investigating the effects of music on brain changes and tissue metabolism.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"25 4","pages":"319-325"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12085213/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144093673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating bovine coronavirus in Indian calves: Incidence, molecular evidence, and pathological role in bovine respiratory disease complex (BRDC).","authors":"B Kamdi, R Singh, V Singh, S Singh, P Kumar, G Kashyap, K Dhama","doi":"10.22099/ijvr.2024.48820.7142","DOIUrl":"10.22099/ijvr.2024.48820.7142","url":null,"abstract":"<p><strong>Background: </strong>Bovine coronavirus (BCoV) and bacterial pathogens contribute to bovine respiratory disease complex (BRDC) in young calves. However, the role of BCoV in BRDC occurrence and site-specific respiratory pathology in India remains poorly explored.</p><p><strong>Aims: </strong>This study aimed to assess BCoV prevalence in BRDC cases.</p><p><strong>Methods: </strong>We investigated 406 weaner calves (166 cattle, 240 buffaloes) up to ≤1 year, with respiratory distress and pulmonary lesions.</p><p><strong>Results: </strong>BRDC cases exhibited 0.98% BCoV occurrence, confirmed by partial <i>N</i> gene amplification (172 bp) via RT-PCR and immunohistochemistry (IHC). Grossly, 4 BCoV positive cases showed variable degrees of consolidation of cranioventral lobes and non-collapsed caudodorsal lobes, associated with congestion and emphysema. Microscopically, the inflated sites of the lung tissue sections showed hallmark changes of interstitial pneumonia characterized by moderate infiltration with lymphocytes and increased numbers of fibroblasts in the interalveolar septa and the stroma of bronchioles and bronchi. In concomitant <i>Pasteurella multocida</i> infected cases, cranioventral lobes exhibited suppurative bronchopneumonia with neutrophilic exudate. The above lesions were well colocalized with BCoV antigen in the epithelial cells and in the debris of the lumen of the alveoli and the bronchi/bronchioles. The sequence comparison of the 172 bp amplicon with the published BCoV <i>N</i> gene showed close relatedness.</p><p><strong>Conclusion: </strong>The present study implicated BCoV as a component of BRDC in India that should be considered in the diagnosis of BRDC outbreaks.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"25 4","pages":"361-367"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12085212/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144093637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immune efficacy of <i>oprH</i> chitosan nanoparticle DNA vaccine against <i>Pseudomonas aeruginosa</i>.","authors":"G Qiang, L Yajing, Z Shiji, T Jiayu, L Jingwen","doi":"10.22099/ijvr.2024.49964.7371","DOIUrl":"10.22099/ijvr.2024.49964.7371","url":null,"abstract":"<p><strong>Background: </strong><i>Pseudomonas aeruginosa</i> is a zoonotic pathogen that poses a threat to human and animal health. However, no vaccine exists for controlling this bacterium.</p><p><strong>Aims: </strong>This study aimed to evaluate the immune efficacy of a chitosan nanoparticle DNA vaccine of the <i>oprH</i> gene from <i>P. aeruginosa</i>.</p><p><strong>Methods: </strong>The naked DNA vaccine based on the <i>oprH</i> gene of <i>P. aeruginosa</i> was constructed. Then, the chitosan nanoparticle DNA vaccine of the <i>oprH</i> gene was prepared and the shape, size, encapsulation efficiency, stability, and ability of anti-DNA enzyme degradation were detected. Chickens were divided into five groups, namely the naked DNA vaccine group (poprH group), chitosan nanoparticle DNA vaccine group (CpoprH group), outer membrane protein vaccine group (OMP group), inactive vaccine group, and PBS group. After being vaccinated with corresponding vaccines, the levels of serum antibodies, lymphocyte proliferation assays, interferon-γ (IFN-γ), interleukin-2 (IL-2), and interleukin-4 (IL-4) concentrations were detected. Groups of chickens were challenged with live virulent <i>P. aeruginosa</i> 2 weeks after the last vaccination and the survival numbers were counted until day 15 post challenge. Then, the protective rates were calculated.</p><p><strong>Results: </strong>The particle size of the chitosan nanoparticle DNA vaccine was approximately 200 nm and close to spherical; the encapsulation efficiency was 95.88%, and it could effectively resist degradation by DNase. Following vaccination, serum antibodies, stimulation index (SI) value, and concentrations of IFN-γ, IL-2, and IL-4 in chickens immunized with the chitosan nanoparticle DNA vaccine were significantly higher than those that were vaccinated with the naked DNA vaccine (P<0.05). The protective rates of poprH, CoprH, OMP vaccine, and inactive vaccine groups were 55%, 75%, 75%, and 90%, respectively.</p><p><strong>Conclusion: </strong>Chitosan could significantly enhance the immune response and protection provided by the naked DNA vaccine of the <i>oprH</i> gene.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"25 4","pages":"353-360"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12085210/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144093566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of carboxymethyl dextran-asparaginase in NALM-6 cell apoptosis and autophagy.","authors":"M Azizian, G H Tamaddon, M Ashrafi, M Chahardahcherik, F Gharechahi","doi":"10.22099/ijvr.2024.49166.7208","DOIUrl":"10.22099/ijvr.2024.49166.7208","url":null,"abstract":"<p><strong>Background: </strong>Acute lymphoblastic leukemia (ALL) is a malignant disorder in both humans and animals. L-asparaginase (L-ASNase) has limitations as a chemotherapy agent due to adverse effects and low serum stability. In a previous study, L-ASNase was chemically modified with carboxymethyl dextran to enhance its properties.</p><p><strong>Aims: </strong>This study aimed to validate the potential of these modifications using the NALM-6 cell line.</p><p><strong>Methods: </strong>NALM-6 cells were cultured and treated with various concentrations, including 0 IU/ml as negative control, 0.5, 1, 1.5, and 2 IU/ml of modified L-ASNase and L-ASNase. The optimal concentration was determined at specific intervals, and viability and metabolic activity were assessed through Trypan blue and MTT tests. Flow cytometry, using Annexin V/PI staining, was employed to evaluate apoptosis. Real-time RT-PCR techniques were used to determine changes in the expression of the <i>ATG2B</i> and <i>LC3-II</i> genes (important genes in autophagy), with data analysis conducted using PRISM software.</p><p><strong>Results: </strong>The modified L-ASNase reduced the viability of NALM-6 cells and induced higher levels of apoptosis (P=0.001). Interestingly, the modified enzyme had a lesser impact on autophagy, which is important for avoiding treatment resistance.</p><p><strong>Conclusion: </strong>The modified L-ASNase showed enhanced effectiveness in reducing the viability of NALM-6 cells and induced higher levels of apoptosis. Interestingly, the modified enzyme had a lesser effect on autophagy, which is important as excessive autophagy can lead to treatment resistance. These findings suggest that the modified L-ASNase may have the potential to be a more effective chemotherapeutic agent for ALL treatments.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"25 4","pages":"344-352"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12085209/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144093569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heat shock protein D1 is up-regulated in various types of canine mammary tumors.","authors":"G Kaur, B V S Kumar, K Gupta","doi":"10.22099/ijvr.2024.50204.7404","DOIUrl":"10.22099/ijvr.2024.50204.7404","url":null,"abstract":"<p><strong>Background: </strong>Mammary tumors are the most common tumors in female dogs. An early diagnosis makes the treatment easier.</p><p><strong>Aims: </strong>The present study aimed to assess heat shock protein D1 (HSPD1) expression in canine mammary tumors.</p><p><strong>Methods: </strong>Canine mammary tumor (CMT) samples were collected from clinics after surgery. Expression of <i>HSPD1</i> transcript in CMT and apparently healthy mammary tissues was analyzed by SYBR green based real-time PCR (qRT-PCR). Further, gene encoding the immuno-dominant region of HSPD1 was cloned using the expression vector pPROEX-HTa and expressed in a prokaryotic system and recombinant HSPD1 (rHSPD1) was purified by affinity chromatography. Hyperimmune serum was raised against rHSPD1 in mice, and immunohistochemistry was standardized to assess the expression of this protein in various histotypes of canine mammary tumors.</p><p><strong>Results: </strong>An elevated <i>HSPD1</i> mRNA expression (5.973 ± 0.862 folds) was observed in canine mammary tumors. Upon purification, a 60 kDa recombinant protein was obtained and confirmed by Western blotting. In 83.3% of healthy mammary tissues, a mild/feeble HSPD1 expression was observed whereas, a strong expression of HSPD1 was seen in 80% CMT samples.</p><p><strong>Conclusion: </strong>The findings suggested that HSPD1 could be used as a molecular marker for canine mammary tumors.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"25 4","pages":"368-375"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12085206/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144093563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nutrient content, <i>in-vitro</i> digestibility, and starch and protein molecular appearance of intact and ammoniated steamed-flaked and/or steamed-infrared heated-flaked barley grain.","authors":"A Honarmand, A R Vakili, M Danesh Mesgaran, A M Tahmasebi","doi":"10.22099/ijvr.2024.49168.7210","DOIUrl":"10.22099/ijvr.2024.49168.7210","url":null,"abstract":"<p><strong>Background: </strong>The impact of different physical and/or chemical treatments in cereal grains on starch morphology and ruminal digestibility has been evaluated.</p><p><strong>Aims: </strong>The effect of chemical and/or physical treatments on starch and protein molecular appearance and the <i>ex-vivo</i> digestibility of barley grain was studied.</p><p><strong>Methods: </strong>Treatments were: steam-flaked barley grain (SFB), SFB treated with ammonium bicarbonate (A), urea (U), and malic acid (M) (SFB_AUM), SFB treated with A, U, and lactic acid (L) (SFB_AUL), steam-infrared heated-flaked barley grain (SIFB), SIFB treated with A, U, and M (SIFB_AUM), and SIFB treated with A, U, and L (SIFB_AUL). Chemicals including A, U, M, and L were used as 56, 8, 10, and 10 g/kg dry matter (DM), respectively. Chemical composition and molecular morphology were determined using scanning electron microscopy and Fourier-transform infrared spectroscopy (FTIR). In situ mobile bag technique and <i>in vitro</i> batch culture procedure were used to estimate ruminal and post-ruminal digestibility.</p><p><strong>Results: </strong>Crude protein (CP) and starch concentrations in SFB_AUL were higher than the others (P<0.05). Starch granule morphology and protein structure were altered in the chemically treated samples. The potentially digestible fraction of DM was the highest in the SFB_AUM (P<0.05). Ruminal disappearance of DM, CP, and starch was improved in SFB_AUL and SIFB_AUL compared with other groups (P<0.05). The highest post-ruminal digestibility of starch and CP was observed in SIFB_AUL and SIFB (P<0.05).</p><p><strong>Conclusion: </strong>Present results indicate that chemical processing with L and applied steam-infrared heated-flaked in barley grain may improve <i>in vitro</i> digestibility of starch and CP and increase granule sizes.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"25 4","pages":"376-387"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12085211/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144093666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Respiratory and intestinal zoonotic cryptosporidiosis in symptomatic domestic pigeons (<i>Columba livia domestica</i>) in Tabriz, Iran.","authors":"M Khordadmehr, A Sazmand, A Oryan, P Almasi, V Ranjbar, A Hemphill, D Otranto","doi":"10.22099/ijvr.2024.49762.7335","DOIUrl":"10.22099/ijvr.2024.49762.7335","url":null,"abstract":"<p><strong>Background: </strong>Domestic pigeons (Columba livia domestica<i>)</i> are the oldest domesticated birds worldwide, harboring many zoonotic parasites and posing potential public health threats.</p><p><strong>Aims: </strong>To investigate cryptosporidiosis in domestic pigeons in Tabriz, Iran, 100 privately owned pigeons presenting weight loss and diarrhea were tested for Cryptosporidium spp. through parasitological, histopathological, and molecular tests.</p><p><strong>Methods: </strong>Modified Ziehl-Neelsen-stained fecal smears and histological sections of the trachea and small intestine were examined microscopically. Genomic DNA of fecal and tracheal specimens was examined by nested conventional PCR targeting <i>18S rDNA</i>, followed by Sanger sequencing of histopathology-confirmed samples and phylogenetic analyses.</p><p><strong>Results: </strong>All pigeons were positive at PCR in their feces and trachea. Oocysts similar to the size of Cryptosporidium species were observed in stained fecal smears of 62% of pigeons. At the histopathological examination, Cryptosporidium<i>-</i>organisms were observed on the apical epithelial surfaces of the small intestine in 84% and trachea in 78% of pigeons. In 23 pigeons, simultaneous tracheal and intestinal cryptosporidiosis was determined. The lesions in affected tracheas and small intestines included hyperemia, villous atrophy and fusion, dilatation of intestinal crypts, irregular epithelial hyperplasia, and sloughing. Diffused mixed inflammatory cell infiltration in the lamina propria was observed, with dominant lymphocytes, plasma cells, and lower numbers of heterophils. Consensus sequences of detected parasites revealed infection with Cryptosporidium parvum and Cryptosporidium meleagridis.</p><p><strong>Conclusion: </strong>Considering the high frequency of cryptosporidiosis reported here in symptomatic birds and that both identified Cryptosporidium species are zoonotic parasites, findings claim a public health risk assessment of this species of animals.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"25 4","pages":"326-332"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12085204/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144093670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing the shelf life of rainbow trout fillets using edible sodium caseinate coating with <i>Oliveria decumbens</i> essential oil nanoemulsion, vitamin E and sodium dodecyl sulfate.","authors":"L Nikravan, S Maktabi, M Ghaderi Ghahfarrokhi, M Mahmoodi Sourestani","doi":"10.22099/ijvr.2024.48884.7185","DOIUrl":"10.22099/ijvr.2024.48884.7185","url":null,"abstract":"<p><strong>Background: </strong>The use of essential oil nanoemulsions has emerged as a promising strategy for extending the shelf life of highly perishable foods, like fish, by leveraging their natural antimicrobial and antioxidant properties.</p><p><strong>Aims: </strong>This study investigated the effects of an edible sodium caseinate coating incorporating <i>Oliveria decumbens</i> essential oil (<i>Od</i>-EO) and its nanoemulsion (<i>Od</i>-NEO), along with vitamin E and sodium dodecyl sulfate (SDS), on the shelf life of rainbow trout fillets during refrigeration.</p><p><strong>Methods: </strong>Fish samples were coated by immersion in pure sodium caseinate (SC), SC+<i>Od</i>-EO, and SC+<i>Od</i>-NEO. Sensory (odor, color, texture, overall acceptance), chemical (pH, total volatile basic nitrogen [TVB-N], thiobarbituric acid [TBA], and peroxide value [PV]), and microbiological analyses (total mesophilic aerobic bacteria, psychrophilic anaerobic bacteria, and <i>Pseudomonas</i> spp. counts) were conducted over 15 days of storage at 4°C.</p><p><strong>Results: </strong>The study results indicated that fillets treated with SC+<i>Od</i>-NEO exhibited significantly higher quality compared with other treatments. Additionally, the combination of nanoemulsion, vitamin E, and SDS showed synergistic effects in reducing microbial growth, delaying lipid oxidation, and improving organoleptic quality, extending the shelf life of rainbow trout fillets up to 15 days. Overall, the correlation analysis highlights the interdependence of microbial, chemical, and sensory parameters and reinforces the role of SC-NEO-based treatments in mitigating spoilage and maintaining the sensory quality of fish fillets during storage.</p><p><strong>Conclusion: </strong>The results highlight the potential of nanoemulsion-based coatings as an innovative and effective strategy for extending the shelf life of highly perishable fish products. Coting with SC+<i>Od</i>-NEO+Vit E+SDS can be recommended as an effective food preservative.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"25 4","pages":"333-343"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12085207/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144093599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}