Iranian journal of veterinary research最新文献

{"title":"Genome-wide association studies for conformation traits in the Turkish Holstein cattle population.","authors":"S Koncagül, A Kasakolu, M Yıldırır, E Ünay, H Koyun, I Karakoyunlu, O Yiğit, A Ö Şen","doi":"10.22099/ijvr.2025.50205.7407","DOIUrl":"10.22099/ijvr.2025.50205.7407","url":null,"abstract":"<p><strong>Background: </strong>Conformation traits (CNTs) are part of the selection goals that significantly affect cow economic efficiency, health, welfare, and productive life in the dairy industry.</p><p><strong>Aims: </strong>This study focused on a genome-wide association study (GWAS) and genetic parameters estimation for 21 CNTs, including udder, leg-foot, body, type, and final classification traits in the Turkish Holstein (THol) dairy cattle population.</p><p><strong>Methods: </strong>A restricted maximum likelihood with a univariate model including the fixed effects of herd-year-season and days in milk was used. The total dataset consisted of CNTs records and Affymetrix BovineSNP54K data for 3,008 THol cows that calved from 2019 to 2022. The gene ontology and Kyoto Encyclopedia of genes and genome pathway databases were used to assign genes to functional categories. The biological pathways were performed in BioMart databases.</p><p><strong>Results: </strong>The heritability of these 21 CNTs ranged from 0.01 (udder index) to 0.133 (udder depth). A total of 16 significant single nucleotide polymorphisms (SNP) associated with 13 CNTs was identified. Significant SNP overlap in the candidate genes, which include <i>ITGB1</i>, <i>TNN</i>, and <i>SEMA3D</i>, have potential for researchers and breeders for CNTs in cattle breeding.</p><p><strong>Conclusion: </strong>These results provide valuable knowledge and contribute to the elucidation of the genetic factors responsible for conformation traits in dairy cattle populations.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"26 1","pages":"59-67"},"PeriodicalIF":1.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12423998/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145064548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular and phenotypic characteristics of isolated <i>Escherichia</i> <i>coli</i> from the skin, gills, and intestine of rainbow trout in retail stores of Kerman, Iran.","authors":"P Mohseni, R Ghanbarpour, M Jajarmi, M Bagheri","doi":"10.22099/ijvr.2025.49736.7331","DOIUrl":"10.22099/ijvr.2025.49736.7331","url":null,"abstract":"<p><strong>Background: </strong><i>Escherichia coli</i> is not naturally present in fish microbiota but can be acquired from sewage-contaminated aquatic environments.</p><p><strong>Aims: </strong>This study was performed to isolate and characterize the <i>E. coli</i> strains in rainbow trout fish marketed for human consumption.</p><p><strong>Methods: </strong>A total number of 166 fish were randomly collected from different retail settings in Kerman, Iran. The fish samples were analyzed to detect <i>E. coli</i> isolates. Antimicrobial resistance genes, Shiga toxin virulence subtypes and phylogenetic sequences were screened by PCR.</p><p><strong>Results: </strong>Prevalence of <i>E. coli</i> isolates on the skin, in the gills and intestine were 76.5% (127/166), 6.6% (11/166), and 3% (5/166), respectively. The most prevalent antimicrobial resistance phenotypes were observed against florfenicol (86.61%), erythromycin (83.46%), flumequine (82.67%), and oxytetracycline (81.88%); and 98.42% of the isolates were multi-drug resistant. The most frequent resistance gene was <i>bla</i> _TEM (14.17%), followed by <i>qnrA</i> (10.23%), <i>tetB</i> (9.44%), <i>sul2</i> (8.66%), <i>bla</i> _SHV (7.87%), <i>sul1</i> (7.87%), <i>dhfr1</i> (3.93%), <i>bla</i> _CTX _-M (3.14%), <i>dhfrV</i> (1.57%), <i>bla</i> _OXA (0.78%). Totally, 8.66% of isolates were categorized into three pathotypes including STEC, EPEC and EHEC. The <i>stx</i> subtypes including <i>stx1a</i>, <i>stx1c</i>, <i>stx1d</i>, <i>stx2c</i>, <i>stx2d</i>, <i>stx2e</i> and <i>stx2f</i> were identified in <i>stx-</i>positive strains. The <i>E. coli</i> isolates were classified into five phylogenetic groups including A (23.62%), B2 (3.93%), D (2.36%), F (9.44%) and cryptic clade I (11.81%).</p><p><strong>Conclusion: </strong>The study revealed that the skin of retail rainbow trout marketed in Kerman may be one of the potential passive carriers of multi-drug resistant and virulent <i>E. coli</i> strains.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"26 1","pages":"17-26"},"PeriodicalIF":1.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12423993/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145064538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of gestational omega-3 supplementation on offspring immunity in goats.","authors":"A Ateş, Ö Öztabak K, H Yardibi, F Esen Gürsel, I Akış, G Atmaca, I B Kalaycilar, I Erez, N Koluman, U Serbester","doi":"10.22099/ijvr.2024.50387.7436","DOIUrl":"10.22099/ijvr.2024.50387.7436","url":null,"abstract":"<p><strong>Background: </strong>Gestational nutrition, particularly in organic dairy goat farming, where natural feeding is mandatory for early gestation, plays a crucial role in determining the health of farm animal offspring. Omega-3 fatty acids are important for extending gestation periods, enhancing neonatal vitality, and increasing birth weights, primarily through their positive impact on colostrum composition.</p><p><strong>Aims: </strong>This study focused on the potential benefits of omega-3 fatty acids for developing passive immunity in offspring.</p><p><strong>Methods: </strong>Sixty-nine pregnant German Fawn x Hair crossbred does were divided into two groups. One group received fish oil (group F, n=35) and the other rumen protected fat (RPF) (group P, n=34) during the first half of gestation. In the second half of gestation, these groups were further split: group FF (n=16) continued on fish oil, while group FP (n=19) switched to RPF; group PP (n=17) remained on RPF, and group PF (n=17) switched to fish oil. Blood was collected from 60 kids at various times post-birth to measure immune factors. Immunoglobulins were quantified using the ELISA method, while biochemical parameters were assessed spectrophotometrically.</p><p><strong>Results: </strong>The PF and PP groups showed significantly higher IgA levels (P<0.05). The PF group also had a significant increase in total protein (P<0.05).</p><p><strong>Conclusion: </strong>The dietary strategy in our study did not positively influence passive immune transfer. Further research is needed to determine the optimal dosage and timing of these supplements to maximize benefits.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"26 1","pages":"51-58"},"PeriodicalIF":1.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12423999/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145064529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intravenous ketamine-medetomidine anesthesia in combination with intrathecal lidocaine for prefemoral coeliotomy in red-eared sliders (<i>Trachemys scripta elegans</i>): A case series study.","authors":"I Lutvikadić, A Šunje-Rizvan, S Kučlar Muftić, A Alić, J Šupić, A Maksimović","doi":"10.22099/ijvr.2025.49481.7275","DOIUrl":"10.22099/ijvr.2025.49481.7275","url":null,"abstract":"<p><strong>Background: </strong>Coeliotomy in chelonian species is performed to gain access to the abdominal cavity and requires general anesthesia. In reptile medicine, alfaxalone and propofol are often preferred, although the usage of these drugs is limited by cost and/or vascular access. Furthermore, according to a recent study, propofol produces significant oxidative stress in red-eared sliders (<i>Trachemys scripta elegans</i>), and in such cases, a ketamine and medetomidine combination may provide a better choice.</p><p><strong>Case description: </strong>Three owned red-eared sliders were separately presented with a history of recent lethargy, inappetence, dyspnea, and restlessness<b>.</b></p><p><strong>Findings/treatment and outcome: </strong>In these three cases of chronic dystocia, a ketamine and medetomidine combination was used to achieve anesthesia induction for surgical treatment. Additionally, intrathecal administration of lidocaine was performed to achieve a potential analgesic effect in deeper intracoelomic reproductive organs. Preoperative changes in blood parameters were observed in one animal, suggesting abnormal kidney function. The same animal showed signs of resedation postoperatively, prolonged recovery, and had a fatal outcome 48 h after the surgery with signs of kidney damage in histopathological evaluation. In the recovery period, none of the surviving animals (n=2) showed neurological or respiratory complications that could be associated with subcarapacial sinus anesthesia administration or intrathecal local anesthesia.</p><p><strong>Conclusion: </strong>According to our observations, intrathecal lidocaine administration provided sufficient analgesia during prefemoral coeliotomy and reproductive organ manipulation. These modes of anesthesia in chelonians require further investigation.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"26 1","pages":"83-87"},"PeriodicalIF":1.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12423995/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145064566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibiotic resistance profiling in <i>Listeria monocytogenes</i> isolates from white meat available in Pakistani retail markets.","authors":"S Gull, T Ahmed, S Sana, M A Shaheen","doi":"10.22099/ijvr.2025.50453.7450","DOIUrl":"10.22099/ijvr.2025.50453.7450","url":null,"abstract":"<p><strong>Background: </strong>Listeriosis is a disease that occurs in immunocompromised people, caused by a zoonotic bacterium, <i>Listeria monocytogenes</i>. The significance of its prevalence in raw meat lies in its potential to cause illness if the meat is undercooked, fails to reach the recommended internal temperature, or through cross-contamination.</p><p><strong>Aims: </strong>This study aims to assess the presence of <i>L. monocytogenes</i> in fresh chicken, fish, and frozen/ready-to-eat (RTE) meat sourced from the retail markets of two major cities in Pakistan.</p><p><strong>Methods: </strong>The identification was done by biochemical and molecular methods targeting two genes of <i>Listeria</i> Pathogenicity Island (LIP-1) i.e., <i>prfa</i> and <i>hly</i> genes by PCR and was sequence analyzed, isolates sequences were submitted to NCBI GenBank to get accession number (ON859912, ON933793, ON933794, ON933795, ON933790, ON933791, and ON933792). The antibiotic resistance of these isolates was confirmed against seven antibiotics using the Disk Diffusion Method.</p><p><strong>Results: </strong>The antibiotic susceptibility profile of these isolates shows that most of the isolates were resistant to Vancomycin (43.2%), Gentamycin (37.8%), and Erythromycin (18.9%).</p><p><strong>Conclusion: </strong>The study reveals a significant presence of <i>L. monocytogenes</i> in meat samples, accompanied by antibiotic resistance to commonly used antibiotics for listeriosis in Pakistan. This alarming situation poses serious hazards to public health.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"26 1","pages":"34-40"},"PeriodicalIF":1.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12424001/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145064511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"IS1311 PCR-RFLP molecular epidemiological approach for genotyping of <i>Mycobacterium avium</i> subspecies <i>paratuberculosis</i> in sheep and goat milk.","authors":"M Dixit, G Filia, S V Singh, M Raies Ul Islam","doi":"10.22099/ijvr.2025.49432.7258","DOIUrl":"10.22099/ijvr.2025.49432.7258","url":null,"abstract":"<p><strong>Background: </strong>Johne's disease, a chronic wasting diarrheal illness, affecting ruminants, poses diagnostic challenges due to the prolonged incubation period of the agent.</p><p><strong>Aims: </strong>The present study aimed to assess the efficacy of the IS900 PCR protocol, for detecting the causative agent and identifying predominant <i>Mycobacterium avium paratuberculosis</i> (MAP) genotypes circulating among small ruminants in the Punjab (India) through the application of IS1311 PCR-RFLP using milk samples.</p><p><strong>Methods: </strong>A total of 205 milk samples, comprising 102 from goats and 103 from sheep, were tested for MAP DNA through IS900 PCR. Samples positive in IS900 PCR assay were subjected to IS1311 PCR assay, using M56 and M119 primers, followed by digestion of the products using <i>Hinf I</i> and <i>Mse I</i> restriction endonuclease enzymes.</p><p><strong>Results: </strong>Of the 205 samples, 37 (16 sheep and 21 goats) tested positive. The IS1311 RFLP analysis of all positive samples exhibited a restriction pattern (67, 218, and 323 bp) corresponding to the Bison type MAP Strain.</p><p><strong>Conclusion: </strong>Identification of a single predominant RFLP type (Bison) in both sheep and goats underscores the potential interspecies transmission and endemic co-circulation of Bison type MAP. These findings emphasize the need for further studies at regional and national levels, offering valuable insights for the development of comprehensive, monitoring and surveillance programs.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"26 1","pages":"1-7"},"PeriodicalIF":1.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12423992/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145064479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanisms in colistin-resistant superbugs transmissible from veterinary, livestock and animal food products to humans.","authors":"N Satarzadeh, A Saraee, Z Hatif Mahdi, A Sadeghi Dousari, M Armanpour, M Taati Moghadam","doi":"10.22099/ijvr.2024.50497.7453","DOIUrl":"10.22099/ijvr.2024.50497.7453","url":null,"abstract":"<p><p>In the era of antibiotic resistance, where multidrug-resistant (MDR), extensively drug resistant (XDR), and pan-drug resistant (PDR) Gram-negative infections are prevalent, it is crucial to identify the primary sources of antibiotic resistance, understand resistant mechanisms, and develop strategies to combat these mechanisms. The emergence of resistance to last-resort antibiotics like colistin has sparked a war between humanity and resistant bacteria, leaving humanity struggling to find effective countermeasures. Although colistin is used as a highly toxic antibiotic in infections that are not treated with routine antibiotics, its widespread use in animal breeding and veterinary medicine has contributed to the spread of colistin-resistant bacteria, plasmid-borne colistin resistance genes (<i>mcr</i>), and antibiotic residues in livestock and animal-derived foods. These sources can potentially transmit colistin resistance to humans through various routes. Therefore, managing the use of colistin in livestock and animal foods, implementing strict monitoring, and establishing guidelines for its proper use are essential to prevent the escalation of colistin resistance. This review article discusses the latest mechanisms of colistin antibiotic resistance, particularly biofilm production as a public health threat, the livestock and animal food sources of this resistance, and the routes of transmission to humans.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"25 4","pages":"298-311"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12085205/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144093663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MHC-linked microsatellite LEI0258 variability and population structure of chicken ecotypes in Iran.","authors":"A Ramezani, G Nikbakht Brujeni, N Sheikhi, K Parvandar Asadollahi","doi":"10.22099/ijvr.2024.50968.7544","DOIUrl":"10.22099/ijvr.2024.50968.7544","url":null,"abstract":"<p><strong>Background: </strong>Investigation of chicken major histocompatibility complex (MHC) genes along with their diversity across native regions and populations, as a genetic resource, can be used in the breeding programs. Important characteristics of MHC genes, such as the association with immunological and production traits, make them exceptional candidates for marker assisted selection.</p><p><strong>Aims: </strong>The aim of this study was to characterize MHC based on the LEI0258 microsatellite marker to evaluate the genetic variability and diversity within and between Iranian chicken populations.</p><p><strong>Methods: </strong>Blood samples were collected from six indigenous ecotypes (n=633) and Ross 308 (n=216) as a commercial breed. The MHC variability was determined based on a microsatellite marker located within MHC, LEI0258. Polymerase chain reaction and fragment analysis was used for microsatellite polymorphism detection.</p><p><strong>Results: </strong>Based on the fragment analysis, 7 alleles were found in Ross 308 and 25 alleles across all 6 populations. The population with the maximum genetic diversity was Mazandaran (0.939), while the population with the minimum genetic diversity was Ross 308 (0.794). Out of the 6 Iranian chicken ecotypes, all except Arian and Khorasan, were in Hardy-Weinberg equilibrium (P<0.05). The genetic variations within (84.92%) and between (15.08%) populations were statistically significant (P<0.001).</p><p><strong>Conclusion: </strong>A significant genetic structure that is not completely homogeneous among the Iranian chicken populations can be considered as distinct genetic resources. This study highlights the value of using markers such as LEI0258 to investigate the diversity of genes that play dual roles in immunity and production.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"25 4","pages":"312-318"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12085208/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144093664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effects of music and darkness on radionuclide distribution during mice FDG-PET scan.","authors":"K Tanha, M Seyedabadi, H Ghadiri, M Ay","doi":"10.22099/ijvr.2024.49641.7306","DOIUrl":"10.22099/ijvr.2024.49641.7306","url":null,"abstract":"<p><strong>Background: </strong>There is growing interest in the therapeutic potential of music or light in different human disorders.</p><p><strong>Aims: </strong>This study aimed to evaluate the effects of music as well as darkness on FDG uptake in 4T1 tumor-bearing BALB/c mice using a PET scan.</p><p><strong>Methods: </strong>The music, darkness, and music plus darkness groups were subjected to either song or darkness and their combination, respectively, 30 min before the radiopharmaceutical injection until the end of the experiments. The control group was imaged in silence under ambient conditions.</p><p><strong>Results: </strong>Our results revealed that music did not significantly alter the range of tumor SUVmean, but showed a slight increase in brain SUVmean (18.2%) and about 100% increase in brain percentage of injected dose per gram (%ID/g) in <i>ex vivo</i> analysis. In contrast, heart SUVmean and heart %ID/g were approximately half those of the silence group. The muscle SUVmean and blood activity measurements showed a decrement upon music exposure. Also, results showed a significant difference in tumor-to-muscle ratio (85% increment) and brain-to-muscle ratio (105% increment) between the silence and music groups. The muscle SUVmean decreased by 50%, and tumor-to-muscle and brain-to-muscle ratios were observed to increase by 44% and 60% in the group exposed to darkness, respectively.</p><p><strong>Conclusion: </strong>Our results suggest that music and environmental factors may influence FDG uptake in small-animal PET imaging, and provide important insights into the reliability of FDG-PET imaging for music intervention research and may aid researchers in investigating the effects of music on brain changes and tissue metabolism.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"25 4","pages":"319-325"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12085213/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144093673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating bovine coronavirus in Indian calves: Incidence, molecular evidence, and pathological role in bovine respiratory disease complex (BRDC).","authors":"B Kamdi, R Singh, V Singh, S Singh, P Kumar, G Kashyap, K Dhama","doi":"10.22099/ijvr.2024.48820.7142","DOIUrl":"10.22099/ijvr.2024.48820.7142","url":null,"abstract":"<p><strong>Background: </strong>Bovine coronavirus (BCoV) and bacterial pathogens contribute to bovine respiratory disease complex (BRDC) in young calves. However, the role of BCoV in BRDC occurrence and site-specific respiratory pathology in India remains poorly explored.</p><p><strong>Aims: </strong>This study aimed to assess BCoV prevalence in BRDC cases.</p><p><strong>Methods: </strong>We investigated 406 weaner calves (166 cattle, 240 buffaloes) up to ≤1 year, with respiratory distress and pulmonary lesions.</p><p><strong>Results: </strong>BRDC cases exhibited 0.98% BCoV occurrence, confirmed by partial <i>N</i> gene amplification (172 bp) via RT-PCR and immunohistochemistry (IHC). Grossly, 4 BCoV positive cases showed variable degrees of consolidation of cranioventral lobes and non-collapsed caudodorsal lobes, associated with congestion and emphysema. Microscopically, the inflated sites of the lung tissue sections showed hallmark changes of interstitial pneumonia characterized by moderate infiltration with lymphocytes and increased numbers of fibroblasts in the interalveolar septa and the stroma of bronchioles and bronchi. In concomitant <i>Pasteurella multocida</i> infected cases, cranioventral lobes exhibited suppurative bronchopneumonia with neutrophilic exudate. The above lesions were well colocalized with BCoV antigen in the epithelial cells and in the debris of the lumen of the alveoli and the bronchi/bronchioles. The sequence comparison of the 172 bp amplicon with the published BCoV <i>N</i> gene showed close relatedness.</p><p><strong>Conclusion: </strong>The present study implicated BCoV as a component of BRDC in India that should be considered in the diagnosis of BRDC outbreaks.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"25 4","pages":"361-367"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12085212/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144093637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}