MHC-linked microsatellite LEI0258 variability and population structure of chicken ecotypes in Iran.

IF 0.8 4区农林科学 Q3 VETERINARY SCIENCES

Iranian journal of veterinary research Pub Date : 2025-01-01 DOI:10.22099/ijvr.2024.50968.7544

A Ramezani, G Nikbakht Brujeni, N Sheikhi, K Parvandar Asadollahi

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引用次数: 0

Abstract

Background: Investigation of chicken major histocompatibility complex (MHC) genes along with their diversity across native regions and populations, as a genetic resource, can be used in the breeding programs. Important characteristics of MHC genes, such as the association with immunological and production traits, make them exceptional candidates for marker assisted selection.

Aims: The aim of this study was to characterize MHC based on the LEI0258 microsatellite marker to evaluate the genetic variability and diversity within and between Iranian chicken populations.

Methods: Blood samples were collected from six indigenous ecotypes (n=633) and Ross 308 (n=216) as a commercial breed. The MHC variability was determined based on a microsatellite marker located within MHC, LEI0258. Polymerase chain reaction and fragment analysis was used for microsatellite polymorphism detection.

Results: Based on the fragment analysis, 7 alleles were found in Ross 308 and 25 alleles across all 6 populations. The population with the maximum genetic diversity was Mazandaran (0.939), while the population with the minimum genetic diversity was Ross 308 (0.794). Out of the 6 Iranian chicken ecotypes, all except Arian and Khorasan, were in Hardy-Weinberg equilibrium (P<0.05). The genetic variations within (84.92%) and between (15.08%) populations were statistically significant (P<0.001).

Conclusion: A significant genetic structure that is not completely homogeneous among the Iranian chicken populations can be considered as distinct genetic resources. This study highlights the value of using markers such as LEI0258 to investigate the diversity of genes that play dual roles in immunity and production.

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mhc连锁微卫星LEI0258在伊朗鸡生态型的变异和种群结构。

背景：研究鸡主要组织相容性复合体（MHC）基因及其在不同地区和种群间的多样性，作为一种遗传资源，可用于育种计划。MHC基因的重要特征，如与免疫和生产性状的关联，使它们成为标记辅助选择的特殊候选者。目的：本研究的目的是基于LEI0258微卫星标记表征MHC，以评估伊朗鸡群体内部和群体之间的遗传变异性和多样性。方法：采集6个地方生态型（n=633）和商业品种罗斯308 （n=216）的血液样本。根据MHC内的微卫星标记LEI0258确定MHC变异性。微卫星多态性检测采用聚合酶链反应和片段分析法。结果：罗斯308共有7个等位基因，6个群体共有25个等位基因。遗传多样性最高的群体为Mazandaran(0.939)，遗传多样性最低的群体为Ross 308（0.794）。在6个伊朗鸡生态型中，除阿里安鸡和呼罗珊鸡外，其余生态型均处于Hardy-Weinberg平衡(p)。结论：伊朗鸡群体间不完全同质的显著遗传结构可被认为是独特的遗传资源。该研究强调了使用LEI0258等标记来研究在免疫和生产中发挥双重作用的基因多样性的价值。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Iranian journal of veterinary research 农林科学-兽医学

CiteScore

1.70

自引率

8.30%

发文量

期刊介绍： The Iranian Journal of Veterinary Research(IJVR) is published quarterly in 4 issues. The aims of this journal are to improve and expand knowledge in all veterinary fields. It is an international journal indexed by the Thomson Institute for Scientific Information (ISI), Elsevier, Scopus, CAB International, Veterinary Bulletin and several other international databases. Research papers and reports on a wide range of veterinary topics are published in the journal after being evaluated by expert reviewers.The Editor-in-Chief is responsible for the editorial content of the journal—including peer-reviewed manuscripts—and the timing of its publication.