Iranian journal of veterinary research最新文献

{"title":"Mito-TEMPO improves preservation of cooled canine semen over a 72-hour period of storage.","authors":"J Jafari, H Ghasemzadeh Nava, A Shahverdi, V Akbarinejad","doi":"10.22099/ijvr.2026.52842.7905","DOIUrl":"10.22099/ijvr.2026.52842.7905","url":null,"abstract":"<p><strong>Background: </strong>The positive effect of Mito-TEMPO, as a mitochondria-targeted antioxidant, on semen preservation has been reported in various species; however, little information is available on canine semen in this regard.</p><p><strong>Aims: </strong>The present study was conducted to evaluate the effect of Mito-TEMPO on preservation of cooled canine semen over a 72 h period of storage.</p><p><strong>Methods: </strong>In study I, the effect of 0 (as control), 25, 50 and 100 μM concentrations of Mito-TEMPO were evaluated on sperm motility, morphology, viability and plasma membrane integrity at time points 0, 24, 48 and 72 h. In study II, the effect of 0 (as control) and 100 μM (as the most effective dose recognized in study I) concentration of Mito-TEMPO was assessed on reactive oxygen species (ROS) and mitochondrial membrane potential at time points 0 and 72 h.</p><p><strong>Results: </strong>In study I, motility, velocity, viability, and proportion of sperm with normal morphology and intact plasma membrane decreased in all groups over time (P<0.05), but this decrease was slower in sperm treated with different doses of Mito-TEMPO than untreated sperm (P<0.05). In this regard, decrease in total and progressive motility observed from 24 h onward in the untreated group; however, such decrease was detected form 48 h onward in groups treated with different doses of Mito-TEMPO (P<0.05). Although all doses of Mito-TEMPO were effective, treatment with 100 μM concentration of Mito-TEMPO provided the best preservation as compared with the control group (P<0.05). Furthermore, study II revealed that ROS generation increased and mitochondrial membrane potential decreased over time in both groups (P<0.05); however, treatment with 100 μM concentration of Mito-TEMPO ameliorated the increase in ROS and the decrease in mitochondrial membrane potential over time (P<0.05).</p><p><strong>Conclusion: </strong>Mito-TEMPO enhances structural and functional characteristics of canine sperm preserved at 4°C for 72 h, and could serve as an effective antioxidant in canine semen extender.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"27 1","pages":"28-37"},"PeriodicalIF":0.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13647971/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149136640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hormonal, biochemical and hematological changes in neonatal calves with sepsis induced by enteritis.","authors":"M Sezer, E Akyüz, Y U Bati, M Kuru, G Gökce","doi":"10.22099/ijvr.2025.52264.7798","DOIUrl":"10.22099/ijvr.2025.52264.7798","url":null,"abstract":"<p><strong>Background: </strong>Diarrhea is one of the most important problems seen in calves during the neonatal period. Sepsis caused by diarrhea is the main cause of death. In the case of sepsis, many hormonal, hematological and biochemical changes occur in calves. It is extremely important to evaluate these parameters together.</p><p><strong>Aims: </strong>The present study aimed to investigate hormonal, biochemical, and hematological changes in neonatal calves affected by septic enteritis to obtain more information about the pathogenesis of sepsis.</p><p><strong>Methods: </strong>The study included a sepsis group consisting of 40 calves aged 0-15 days with enteritis, and a control group composed of 20 clinically and hematologically healthy calves of similar age. Calves presenting with enteritis, anorexia, severe dehydration, and general condition deterioration were examined, and those meeting the sepsis criteria were included in the study. Hematological, hormonal, and biochemical measurements were performed on blood samples obtained from the calves. Additionally, pathogenic agents causing enteritis were identified using immunochromatographic methods on fecal samples.</p><p><strong>Results: </strong>In the sepsis group, creatinine, total protein, and urea levels, as well as the enzymatic activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), gamma-glutamyl transferase (GGT), and creatine kinase (CK) were significantly higher compared with the healthy group (P<0.05), while cholesterol levels were significantly lower (P<0.001). The total leukocyte count (WBC), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), and granulocyte count were also found to be higher in the sepsis group compared with the control group (P<0.05). Furthermore, calves in the sepsis group were determined to be hypothermic, tachycardic and tachypneic compared with the control group (P<0.05). In the sepsis group, notable reductions in free T3, free T4 and erythropoietin (EPO) levels were observed (P<0.05). However, cortisol, insulin, and adrenocorticotropic hormone (ACTH) levels were significantly higher than those in the healthy group (P<0.05).</p><p><strong>Conclusion: </strong>Sepsis causes significant hematological, biochemical and hormonal changes in neonatal calves.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"27 1","pages":"71-80"},"PeriodicalIF":0.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13647944/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149136587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide association studies for conformation traits in the Turkish Holstein cattle population.","authors":"S Koncagül, A Kasakolu, M Yıldırır, E Ünay, H Koyun, I Karakoyunlu, O Yiğit, A Ö Şen","doi":"10.22099/ijvr.2025.50205.7407","DOIUrl":"10.22099/ijvr.2025.50205.7407","url":null,"abstract":"<p><strong>Background: </strong>Conformation traits (CNTs) are part of the selection goals that significantly affect cow economic efficiency, health, welfare, and productive life in the dairy industry.</p><p><strong>Aims: </strong>This study focused on a genome-wide association study (GWAS) and genetic parameters estimation for 21 CNTs, including udder, leg-foot, body, type, and final classification traits in the Turkish Holstein (THol) dairy cattle population.</p><p><strong>Methods: </strong>A restricted maximum likelihood with a univariate model including the fixed effects of herd-year-season and days in milk was used. The total dataset consisted of CNTs records and Affymetrix BovineSNP54K data for 3,008 THol cows that calved from 2019 to 2022. The gene ontology and Kyoto Encyclopedia of genes and genome pathway databases were used to assign genes to functional categories. The biological pathways were performed in BioMart databases.</p><p><strong>Results: </strong>The heritability of these 21 CNTs ranged from 0.01 (udder index) to 0.133 (udder depth). A total of 16 significant single nucleotide polymorphisms (SNP) associated with 13 CNTs was identified. Significant SNP overlap in the candidate genes, which include <i>ITGB1</i>, <i>TNN</i>, and <i>SEMA3D</i>, have potential for researchers and breeders for CNTs in cattle breeding.</p><p><strong>Conclusion: </strong>These results provide valuable knowledge and contribute to the elucidation of the genetic factors responsible for conformation traits in dairy cattle populations.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"26 1","pages":"59-67"},"PeriodicalIF":1.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12423998/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145064548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of supplementing rosemary essential oil and selenium yeast in the diet on sperm parameters and antioxidant activity of semen in older broiler breeder roosters.","authors":"S Barzegar, S Tabatabaei Vakili, Kh Mirzadeh, S Salari, Sh Hosseinifar","doi":"10.22099/ijvr.2025.51978.7746","DOIUrl":"10.22099/ijvr.2025.51978.7746","url":null,"abstract":"<p><strong>Background: </strong>Aging is associated with various physiological changes that lead to oxidative stress and the production of reactive oxygen species (ROS), negatively affecting sperm structure and function.</p><p><strong>Aims: </strong>This study aimed to investigate the impact of incorporating rosemary essential oil and selenium yeast into the diet on sperm quality parameters, antioxidant enzyme activity, and lipid peroxidation rates of semen in aged broiler breeder roosters.</p><p><strong>Methods: </strong>Thirty-six 57-week-old Ross 308 roosters were randomly assigned in a 3×2 factorial arrangement, in six treatments with six replications. The treatments included three levels of rosemary essential oil (0, 100, and 200 mg/kg) and two levels of selenium yeast (0 and 0.3 mg/kg) for 7 weeks. Sperm parameters, antioxidant enzyme activities, and lipid peroxidation levels in seminal plasma were evaluated.</p><p><strong>Results: </strong>The results showed significant effects of rosemary essential oil and selenium yeast on sperm parameters. 100 mg rosemary essential oil plus 0.3 mg selenium yeast/kg of diet showed the most significant improvement in sperm quality (P<0.05). However, there was no significant difference in sperm morphological abnormalities among the treatments. Additionally, seminal plasma antioxidant enzyme activities, including glutathione peroxidase (GPx), superoxide dismutase (SOD), catalase (CAT), and total antioxidant capacity (TAC) significantly increased and malondialdehyde (MDA) decreased, particularly in the treatment containing 100 mg rosemary essential oil plus 0.3 mg selenium yeast/kg diet (P<0.05).</p><p><strong>Conclusion: </strong>Overall, 100 mg/kg rosemary essential oil combined with 0.3 mg/kg selenium yeast in the diet is recommended for aged broiler breeder roosters to enhance their reproductive function.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"26 4","pages":"341-349"},"PeriodicalIF":0.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13426090/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148653773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular and phenotypic characteristics of isolated <i>Escherichia</i> <i>coli</i> from the skin, gills, and intestine of rainbow trout in retail stores of Kerman, Iran.","authors":"P Mohseni, R Ghanbarpour, M Jajarmi, M Bagheri","doi":"10.22099/ijvr.2025.49736.7331","DOIUrl":"10.22099/ijvr.2025.49736.7331","url":null,"abstract":"<p><strong>Background: </strong><i>Escherichia coli</i> is not naturally present in fish microbiota but can be acquired from sewage-contaminated aquatic environments.</p><p><strong>Aims: </strong>This study was performed to isolate and characterize the <i>E. coli</i> strains in rainbow trout fish marketed for human consumption.</p><p><strong>Methods: </strong>A total number of 166 fish were randomly collected from different retail settings in Kerman, Iran. The fish samples were analyzed to detect <i>E. coli</i> isolates. Antimicrobial resistance genes, Shiga toxin virulence subtypes and phylogenetic sequences were screened by PCR.</p><p><strong>Results: </strong>Prevalence of <i>E. coli</i> isolates on the skin, in the gills and intestine were 76.5% (127/166), 6.6% (11/166), and 3% (5/166), respectively. The most prevalent antimicrobial resistance phenotypes were observed against florfenicol (86.61%), erythromycin (83.46%), flumequine (82.67%), and oxytetracycline (81.88%); and 98.42% of the isolates were multi-drug resistant. The most frequent resistance gene was <i>bla</i> _TEM (14.17%), followed by <i>qnrA</i> (10.23%), <i>tetB</i> (9.44%), <i>sul2</i> (8.66%), <i>bla</i> _SHV (7.87%), <i>sul1</i> (7.87%), <i>dhfr1</i> (3.93%), <i>bla</i> _CTX _-M (3.14%), <i>dhfrV</i> (1.57%), <i>bla</i> _OXA (0.78%). Totally, 8.66% of isolates were categorized into three pathotypes including STEC, EPEC and EHEC. The <i>stx</i> subtypes including <i>stx1a</i>, <i>stx1c</i>, <i>stx1d</i>, <i>stx2c</i>, <i>stx2d</i>, <i>stx2e</i> and <i>stx2f</i> were identified in <i>stx-</i>positive strains. The <i>E. coli</i> isolates were classified into five phylogenetic groups including A (23.62%), B2 (3.93%), D (2.36%), F (9.44%) and cryptic clade I (11.81%).</p><p><strong>Conclusion: </strong>The study revealed that the skin of retail rainbow trout marketed in Kerman may be one of the potential passive carriers of multi-drug resistant and virulent <i>E. coli</i> strains.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"26 1","pages":"17-26"},"PeriodicalIF":1.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12423993/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145064538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of gestational omega-3 supplementation on offspring immunity in goats.","authors":"A Ateş, Ö Öztabak K, H Yardibi, F Esen Gürsel, I Akış, G Atmaca, I B Kalaycilar, I Erez, N Koluman, U Serbester","doi":"10.22099/ijvr.2024.50387.7436","DOIUrl":"10.22099/ijvr.2024.50387.7436","url":null,"abstract":"<p><strong>Background: </strong>Gestational nutrition, particularly in organic dairy goat farming, where natural feeding is mandatory for early gestation, plays a crucial role in determining the health of farm animal offspring. Omega-3 fatty acids are important for extending gestation periods, enhancing neonatal vitality, and increasing birth weights, primarily through their positive impact on colostrum composition.</p><p><strong>Aims: </strong>This study focused on the potential benefits of omega-3 fatty acids for developing passive immunity in offspring.</p><p><strong>Methods: </strong>Sixty-nine pregnant German Fawn x Hair crossbred does were divided into two groups. One group received fish oil (group F, n=35) and the other rumen protected fat (RPF) (group P, n=34) during the first half of gestation. In the second half of gestation, these groups were further split: group FF (n=16) continued on fish oil, while group FP (n=19) switched to RPF; group PP (n=17) remained on RPF, and group PF (n=17) switched to fish oil. Blood was collected from 60 kids at various times post-birth to measure immune factors. Immunoglobulins were quantified using the ELISA method, while biochemical parameters were assessed spectrophotometrically.</p><p><strong>Results: </strong>The PF and PP groups showed significantly higher IgA levels (P<0.05). The PF group also had a significant increase in total protein (P<0.05).</p><p><strong>Conclusion: </strong>The dietary strategy in our study did not positively influence passive immune transfer. Further research is needed to determine the optimal dosage and timing of these supplements to maximize benefits.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"26 1","pages":"51-58"},"PeriodicalIF":1.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12423999/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145064529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intravenous ketamine-medetomidine anesthesia in combination with intrathecal lidocaine for prefemoral coeliotomy in red-eared sliders (<i>Trachemys scripta elegans</i>): A case series study.","authors":"I Lutvikadić, A Šunje-Rizvan, S Kučlar Muftić, A Alić, J Šupić, A Maksimović","doi":"10.22099/ijvr.2025.49481.7275","DOIUrl":"10.22099/ijvr.2025.49481.7275","url":null,"abstract":"<p><strong>Background: </strong>Coeliotomy in chelonian species is performed to gain access to the abdominal cavity and requires general anesthesia. In reptile medicine, alfaxalone and propofol are often preferred, although the usage of these drugs is limited by cost and/or vascular access. Furthermore, according to a recent study, propofol produces significant oxidative stress in red-eared sliders (<i>Trachemys scripta elegans</i>), and in such cases, a ketamine and medetomidine combination may provide a better choice.</p><p><strong>Case description: </strong>Three owned red-eared sliders were separately presented with a history of recent lethargy, inappetence, dyspnea, and restlessness<b>.</b></p><p><strong>Findings/treatment and outcome: </strong>In these three cases of chronic dystocia, a ketamine and medetomidine combination was used to achieve anesthesia induction for surgical treatment. Additionally, intrathecal administration of lidocaine was performed to achieve a potential analgesic effect in deeper intracoelomic reproductive organs. Preoperative changes in blood parameters were observed in one animal, suggesting abnormal kidney function. The same animal showed signs of resedation postoperatively, prolonged recovery, and had a fatal outcome 48 h after the surgery with signs of kidney damage in histopathological evaluation. In the recovery period, none of the surviving animals (n=2) showed neurological or respiratory complications that could be associated with subcarapacial sinus anesthesia administration or intrathecal local anesthesia.</p><p><strong>Conclusion: </strong>According to our observations, intrathecal lidocaine administration provided sufficient analgesia during prefemoral coeliotomy and reproductive organ manipulation. These modes of anesthesia in chelonians require further investigation.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"26 1","pages":"83-87"},"PeriodicalIF":1.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12423995/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145064566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular typing of <i>Dichelobacter nodosus</i> in ovine footrot and expression of its fimbrial biogenesis protein PilQ as a candidate footrot vaccine.","authors":"M Umul Qurra, Md Ishfaq Hussain, S Qureshi, S Farooq, M Nadeem Hassan, A H Wani, M A Bhat, Z A Kashoo, M S Banday, G Badroo, M Nabi, D Deka","doi":"10.22099/ijvr.2025.50574.7468","DOIUrl":"10.22099/ijvr.2025.50574.7468","url":null,"abstract":"<p><strong>Background: </strong>Footrot is a highly contagious disease of the feet in sheep and goats.</p><p><strong>Aims: </strong>The study was conducted to determine the serogroup distribution of <i>Dichelobacter nodosus</i> in sheep affected by footrot on organized farms and flocks reared by farmers across four districts of the Kashmir Valley.</p><p><strong>Methods: </strong>Sheep from randomly selected organized farms and flocks raised by farmers and the Bakerwal tribes were screened for footrot lesions based on clinical signs, particularly lameness. Two hundred sixty swab samples were collected from sheep exhibiting footrot lesions, and <i>D. nodosus</i> infection was confirmed using 16S rRNA-specific PCR. For serogroup subtyping, multiplex PCR was performed. Furthermore, we utilized the pET28a expression vector to clone and express the outer membrane fimbrial protein antigen, which was confirmed using SDS-PAGE and Western blot analysis.</p><p><strong>Results: </strong><i>D. nodosus</i> was detected in 149 samples by 16S rRNA-specific PCR. Serogroups B and E were detected in 85.9% and 10.74% of positive samples, respectively, while both serogroups were detected in 3.36% of the samples. The outer membrane domain containing the major antigenic epitopes of the <i>pilQ</i> gene of <i>D. nodosus</i> was successfully cloned into the pET28a expression vector.</p><p><strong>Conclusion: </strong>The prevalence of ovine footrot was found to be low during the study period. Serogroup B of <i>D. nodosus</i> was found to be predominant, while serogroup E was detected exclusively in some isolated flocks reared by farmers. The <i>pilQ</i> gene was successfully expressed in <i>Escherichia coli</i> and purified to near homogeneity, making it suitable for further exploitation as a serogroup-independent vaccine candidate against footrot.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"26 4","pages":"398-406"},"PeriodicalIF":0.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13426106/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148653778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibiotic resistance profiling in <i>Listeria monocytogenes</i> isolates from white meat available in Pakistani retail markets.","authors":"S Gull, T Ahmed, S Sana, M A Shaheen","doi":"10.22099/ijvr.2025.50453.7450","DOIUrl":"10.22099/ijvr.2025.50453.7450","url":null,"abstract":"<p><strong>Background: </strong>Listeriosis is a disease that occurs in immunocompromised people, caused by a zoonotic bacterium, <i>Listeria monocytogenes</i>. The significance of its prevalence in raw meat lies in its potential to cause illness if the meat is undercooked, fails to reach the recommended internal temperature, or through cross-contamination.</p><p><strong>Aims: </strong>This study aims to assess the presence of <i>L. monocytogenes</i> in fresh chicken, fish, and frozen/ready-to-eat (RTE) meat sourced from the retail markets of two major cities in Pakistan.</p><p><strong>Methods: </strong>The identification was done by biochemical and molecular methods targeting two genes of <i>Listeria</i> Pathogenicity Island (LIP-1) i.e., <i>prfa</i> and <i>hly</i> genes by PCR and was sequence analyzed, isolates sequences were submitted to NCBI GenBank to get accession number (ON859912, ON933793, ON933794, ON933795, ON933790, ON933791, and ON933792). The antibiotic resistance of these isolates was confirmed against seven antibiotics using the Disk Diffusion Method.</p><p><strong>Results: </strong>The antibiotic susceptibility profile of these isolates shows that most of the isolates were resistant to Vancomycin (43.2%), Gentamycin (37.8%), and Erythromycin (18.9%).</p><p><strong>Conclusion: </strong>The study reveals a significant presence of <i>L. monocytogenes</i> in meat samples, accompanied by antibiotic resistance to commonly used antibiotics for listeriosis in Pakistan. This alarming situation poses serious hazards to public health.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"26 1","pages":"34-40"},"PeriodicalIF":1.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12424001/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145064511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"IS1311 PCR-RFLP molecular epidemiological approach for genotyping of <i>Mycobacterium avium</i> subspecies <i>paratuberculosis</i> in sheep and goat milk.","authors":"M Dixit, G Filia, S V Singh, M Raies Ul Islam","doi":"10.22099/ijvr.2025.49432.7258","DOIUrl":"10.22099/ijvr.2025.49432.7258","url":null,"abstract":"<p><strong>Background: </strong>Johne's disease, a chronic wasting diarrheal illness, affecting ruminants, poses diagnostic challenges due to the prolonged incubation period of the agent.</p><p><strong>Aims: </strong>The present study aimed to assess the efficacy of the IS900 PCR protocol, for detecting the causative agent and identifying predominant <i>Mycobacterium avium paratuberculosis</i> (MAP) genotypes circulating among small ruminants in the Punjab (India) through the application of IS1311 PCR-RFLP using milk samples.</p><p><strong>Methods: </strong>A total of 205 milk samples, comprising 102 from goats and 103 from sheep, were tested for MAP DNA through IS900 PCR. Samples positive in IS900 PCR assay were subjected to IS1311 PCR assay, using M56 and M119 primers, followed by digestion of the products using <i>Hinf I</i> and <i>Mse I</i> restriction endonuclease enzymes.</p><p><strong>Results: </strong>Of the 205 samples, 37 (16 sheep and 21 goats) tested positive. The IS1311 RFLP analysis of all positive samples exhibited a restriction pattern (67, 218, and 323 bp) corresponding to the Bison type MAP Strain.</p><p><strong>Conclusion: </strong>Identification of a single predominant RFLP type (Bison) in both sheep and goats underscores the potential interspecies transmission and endemic co-circulation of Bison type MAP. These findings emphasize the need for further studies at regional and national levels, offering valuable insights for the development of comprehensive, monitoring and surveillance programs.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"26 1","pages":"1-7"},"PeriodicalIF":1.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12423992/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145064479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}