Immune efficacy of oprH chitosan nanoparticle DNA vaccine against Pseudomonas aeruginosa.

IF 0.8 4区农林科学 Q3 VETERINARY SCIENCES

Iranian journal of veterinary research Pub Date : 2025-01-01 DOI:10.22099/ijvr.2024.49964.7371

G Qiang, L Yajing, Z Shiji, T Jiayu, L Jingwen

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引用次数: 0

Abstract

Background: Pseudomonas aeruginosa is a zoonotic pathogen that poses a threat to human and animal health. However, no vaccine exists for controlling this bacterium.

Aims: This study aimed to evaluate the immune efficacy of a chitosan nanoparticle DNA vaccine of the oprH gene from P. aeruginosa.

Methods: The naked DNA vaccine based on the oprH gene of P. aeruginosa was constructed. Then, the chitosan nanoparticle DNA vaccine of the oprH gene was prepared and the shape, size, encapsulation efficiency, stability, and ability of anti-DNA enzyme degradation were detected. Chickens were divided into five groups, namely the naked DNA vaccine group (poprH group), chitosan nanoparticle DNA vaccine group (CpoprH group), outer membrane protein vaccine group (OMP group), inactive vaccine group, and PBS group. After being vaccinated with corresponding vaccines, the levels of serum antibodies, lymphocyte proliferation assays, interferon-γ (IFN-γ), interleukin-2 (IL-2), and interleukin-4 (IL-4) concentrations were detected. Groups of chickens were challenged with live virulent P. aeruginosa 2 weeks after the last vaccination and the survival numbers were counted until day 15 post challenge. Then, the protective rates were calculated.

Results: The particle size of the chitosan nanoparticle DNA vaccine was approximately 200 nm and close to spherical; the encapsulation efficiency was 95.88%, and it could effectively resist degradation by DNase. Following vaccination, serum antibodies, stimulation index (SI) value, and concentrations of IFN-γ, IL-2, and IL-4 in chickens immunized with the chitosan nanoparticle DNA vaccine were significantly higher than those that were vaccinated with the naked DNA vaccine (P<0.05). The protective rates of poprH, CoprH, OMP vaccine, and inactive vaccine groups were 55%, 75%, 75%, and 90%, respectively.

Conclusion: Chitosan could significantly enhance the immune response and protection provided by the naked DNA vaccine of the oprH gene.

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oprH壳聚糖纳米颗粒DNA疫苗对铜绿假单胞菌的免疫效果。

背景：铜绿假单胞菌是一种威胁人类和动物健康的人畜共患病原体。然而，目前还没有控制这种细菌的疫苗。目的：研究铜绿假单胞菌oprH基因壳聚糖纳米粒DNA疫苗的免疫效果。方法：构建铜绿假单胞菌oprH基因裸DNA疫苗。制备了oprH基因的壳聚糖纳米粒DNA疫苗，并对其形状、大小、包封效率、稳定性和抗DNA酶降解能力进行了检测。将鸡分为5组，分别为裸DNA疫苗组（poprH组）、壳聚糖纳米颗粒DNA疫苗组（CpoprH组）、外膜蛋白疫苗组（OMP组）、灭活疫苗组和PBS组。接种相应疫苗后，检测血清抗体水平、淋巴细胞增殖试验、干扰素-γ (IFN-γ)、白细胞介素-2 （IL-2）和白细胞介素-4 （IL-4）浓度。在最后一次接种后2周，用毒力强的铜绿假单胞菌攻毒各组鸡，计数存活数，直至攻毒后第15天。然后，计算出保护率。结果：壳聚糖纳米颗粒DNA疫苗的粒径约为200 nm，接近球形；包封率为95.88%，能有效抵抗dna酶的降解。接种后，经壳聚糖纳米颗粒DNA疫苗免疫的鸡血清抗体、刺激指数（SI）值以及IFN-γ、IL-2和IL-4浓度均显著高于裸DNA疫苗(p)。结论：壳聚糖可显著增强oprH基因裸DNA疫苗的免疫应答和保护作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Iranian journal of veterinary research 农林科学-兽医学

CiteScore

1.70

自引率

8.30%

发文量

期刊介绍： The Iranian Journal of Veterinary Research(IJVR) is published quarterly in 4 issues. The aims of this journal are to improve and expand knowledge in all veterinary fields. It is an international journal indexed by the Thomson Institute for Scientific Information (ISI), Elsevier, Scopus, CAB International, Veterinary Bulletin and several other international databases. Research papers and reports on a wide range of veterinary topics are published in the journal after being evaluated by expert reviewers.The Editor-in-Chief is responsible for the editorial content of the journal—including peer-reviewed manuscripts—and the timing of its publication.