Antibiotic resistance profiling in Listeria monocytogenes isolates from white meat available in Pakistani retail markets.

IF 1 4区农林科学 Q3 VETERINARY SCIENCES

Iranian journal of veterinary research Pub Date : 2025-01-01 DOI:10.22099/ijvr.2025.50453.7450

S Gull, T Ahmed, S Sana, M A Shaheen

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Abstract

Background: Listeriosis is a disease that occurs in immunocompromised people, caused by a zoonotic bacterium, Listeria monocytogenes. The significance of its prevalence in raw meat lies in its potential to cause illness if the meat is undercooked, fails to reach the recommended internal temperature, or through cross-contamination.

Aims: This study aims to assess the presence of L. monocytogenes in fresh chicken, fish, and frozen/ready-to-eat (RTE) meat sourced from the retail markets of two major cities in Pakistan.

Methods: The identification was done by biochemical and molecular methods targeting two genes of Listeria Pathogenicity Island (LIP-1) i.e., prfa and hly genes by PCR and was sequence analyzed, isolates sequences were submitted to NCBI GenBank to get accession number (ON859912, ON933793, ON933794, ON933795, ON933790, ON933791, and ON933792). The antibiotic resistance of these isolates was confirmed against seven antibiotics using the Disk Diffusion Method.

Results: The antibiotic susceptibility profile of these isolates shows that most of the isolates were resistant to Vancomycin (43.2%), Gentamycin (37.8%), and Erythromycin (18.9%).

Conclusion: The study reveals a significant presence of L. monocytogenes in meat samples, accompanied by antibiotic resistance to commonly used antibiotics for listeriosis in Pakistan. This alarming situation poses serious hazards to public health.

Abstract Image

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巴基斯坦零售市场上可买到的白肉中分离的单核细胞增生李斯特菌的抗生素耐药性分析

背景：李斯特菌病是一种发生在免疫功能低下人群中的疾病，由一种人畜共患细菌，单核细胞增生李斯特菌引起。它在生肉中普遍存在的意义在于，如果肉未煮熟，未达到建议的内部温度，或通过交叉污染，它可能会导致疾病。目的：本研究旨在评估来自巴基斯坦两个主要城市零售市场的新鲜鸡肉、鱼类和冷冻/即食（RTE）肉类中单核细胞增生乳杆菌的存在。方法：采用生化和分子方法对李斯特菌致病性岛（LIP-1）的两个基因prfa和hly基因进行PCR鉴定，并对分离株序列进行测序，将分离株序列提交NCBI GenBank获取登录号（ON859912、ON933793、ON933794、ON933795、ON933790、ON933791和ON933792）。采用纸片扩散法对7种抗生素进行耐药性鉴定。结果：各分离株对万古霉素（43.2%）、庆大霉素（37.8%）和红霉素（18.9%）均耐药。结论：该研究揭示了肉类样品中单核细胞增生乳杆菌的显著存在，并伴有对巴基斯坦李斯特菌病常用抗生素的耐药性。这一令人震惊的情况对公众健康构成严重危害。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Iranian journal of veterinary research 农林科学-兽医学

CiteScore

1.70

自引率

8.30%

发文量

期刊介绍： The Iranian Journal of Veterinary Research(IJVR) is published quarterly in 4 issues. The aims of this journal are to improve and expand knowledge in all veterinary fields. It is an international journal indexed by the Thomson Institute for Scientific Information (ISI), Elsevier, Scopus, CAB International, Veterinary Bulletin and several other international databases. Research papers and reports on a wide range of veterinary topics are published in the journal after being evaluated by expert reviewers.The Editor-in-Chief is responsible for the editorial content of the journal—including peer-reviewed manuscripts—and the timing of its publication.